CN105193680B - Short Radix Sangusorbae extract and its application in cosmetics are prepared - Google Patents

Short Radix Sangusorbae extract and its application in cosmetics are prepared Download PDF

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Publication number
CN105193680B
CN105193680B CN201510728084.5A CN201510728084A CN105193680B CN 105193680 B CN105193680 B CN 105193680B CN 201510728084 A CN201510728084 A CN 201510728084A CN 105193680 B CN105193680 B CN 105193680B
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short
radix sangusorbae
extract
skin
cosmetics
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CN105193680A (en
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杨立新
许刚
刘爱忠
裴盛基
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Yunnan West Grass Resources Development Co ltd
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Kunming Institute of Botany of CAS
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
    • A61K36/739—Sanguisorba (burnet)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/0012—Galenical forms characterised by the site of application
    • A61K9/0043—Nose
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/08—Solutions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/10—Dispersions; Emulsions
    • A61K9/12—Aerosols; Foams
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2013—Organic compounds, e.g. phospholipids, fats
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/2031—Organic macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyethylene glycol, polyethylene oxide, poloxamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/4841—Filling excipients; Inactive ingredients
    • A61K9/4858—Organic compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

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Abstract

The invention discloses short garden burnet (Sanguisorba officinalis L.) plant extracts and utilize it as the cosmetic additive agent of skin-whitening, moisturizing and antioxidation activity and its application in cosmetics.The short Radix Sangusorbae extract is short garden burnet herb or the ethanol extract of root, which has good DPPH radical scavenging activities, is good natural.In terms of human body skin care, short Radix Sangusorbae extract possesses the cosmetics for skin application potential such as outstanding crease-resistant, whitening, sun-proof.The present invention efficiently uses Naxi's tradition medicinal plant --- short garden burnet, the short Radix Sangusorbae extract with very high cosmetics for skin activity is obtained, these extracts, which can be used for making, has good antioxidant, possess the functions such as outstanding crease-resistant, whitening, sun-proof, be good natural plants cosmetic material.

Description

Short Radix Sangusorbae extract and its application in cosmetics are prepared
Technical field
The invention belongs to cosmetics and pharmaceutical technology field, and in particular to the short garden burnet of medicinal plant passed from a kind of Naxi Middle extraction natural active matter -- short Radix Sangusorbae extract is extracted using it as the cosmetics or drug of active ingredient and short garden burnet Application of the object in a variety of cosmetics for skin such as moisturizing, tender skin, crease-resistant, sun-proof, whitening are prepared also is preparing treatment or prevention Application in dermopathic drug
Technical background
Short garden burnet (Sanguisorba filiformis (Hook.f.) Hand.-Mazz.) is rose family garden burnet platymiscium, Herbaceos perennial, root is cylindrical, and surface sepia, basal leaf is pinnate compound leaf, there is 3-5 pairs of leaflet, and petiole is smooth, leaflet Piece has short handle, and dilute there are few handle, width egg shape or subcircular, flower unisexuality, monoecism, inflorescence head, several spherical shapes.Short garden burnet is grown on The area that 1,200 meters to 4,000 meters of height above sea level, is grown in patana marsh more, and Yunnan, Sichuan, Tibet etc. are common in China Cultivation is quoted in ground there has been no artificial at present.
Short garden burnet is the medicinal plant that Naxi is important in Chinese Minority Nationalities, is the important raw and processed materials of Nahsi medicine.Shortly Elm is mainly used for the diseases such as dysmenorrhoea, early in《Yunnan medicine record》With《Dali provides will》In just it is on the books：" short garden burnet, five mother Lao Bao：Root cures mainly Irregular menstruation, fertility, deficient qi and blood ".Except in the medicine of Nahsi, China is more rare to the use of short garden burnet, yet there are no There is short garden burnet either short Radix Sangusorbae extract report active in cosmetics for skin or drug.
The content of the invention
Present invention aims at short Radix Sangusorbae extract is provided, using it as the cosmetics of active ingredient, their preparation method, And its application in cosmetics are prepared.The present invention specifies that short Radix Sangusorbae extract has good skin effect and security, Become good natural skin care cosmetic raw materials.
In order to realize the above-mentioned purpose of the present invention, the present invention provides following technical solutions：
Short Radix Sangusorbae extract, is prepared by following methods：Short garden burnet herb or root are taken, is smashed, with short garden burnet The 15-30 times of 85%-95% ethyl alcohol cold soaking 18-28h measured, collect filtrate, repeat 2-5 time, filtrate decompression distillation, concentration filter Liquid, low temperature drying obtain crude extract, and crude extract is decolourized through macroporous resin adsorption, and after 85%-95% ethanol elutions, concentration is evaporated, Frozen drying obtains short Radix Sangusorbae extract.
The present invention also provides a kind of cosmetics, are made of above-mentioned short Radix Sangusorbae extract and cosmetics common carrier, institute The carrier stated is selected from one or more Aquo Systems, glycerine, C1-4 alcohol, fatty alcohol, polyalcohol, aliphatic ether, fatty ester, second two Alcohol, propylene glycol, vegetable oil, mineral oil, liposome, laminar lipid materials, silicone oil, water or combination thereof.
And application of the short Radix Sangusorbae extract in cosmetics are prepared.
The short Radix Sangusorbae extract prepare moisturizing tender skin is crease-resistant or sun-proof or skin-lightening cosmetic in application.
The short Radix Sangusorbae extract is preparing anesthetic, antifungal agent, anti-inflammatory agent, antioxidant, preservative, chelating Agent, colorant, decolorising agent, softening agent, emulsifier, agents for defoliating, film forming agent, aromatic, wetting agent, pest repellant, lubricant, humidification Agent, medicament, light stabilizer, preservative, Derma-Guard, skin penetration enhancer, sun-screening agent, stabilizer, surfactant, increasing Thick dose, viscosity modifier, the application in vitamin.
The short Radix Sangusorbae extract prepare anesthetic, antifungal agent, anti-inflammatory agent, preservative, chelating agent, colorant, Decolorising agent, softening agent, emulsifier, agents for defoliating, film forming agent, aromatic, wetting agent, pest repellant, lubricant, humidizer, medicament, light Stabilizer, preservative, Derma-Guard, skin penetration enhancer, sun-screening agent, stabilizer, surfactant, thickener, viscosity Application in conditioning agent, vitamin.
Application of the short Radix Sangusorbae extract in antioxidant is prepared.
The present invention also provides a kind of prevention or treat dermopathic pharmaceutical composition, by the short Radix Sangusorbae extract and Drug common carrier forms.
Application of the short Radix Sangusorbae extract of the present invention in preparing prevention or treating dermopathic drug.
Invention also provides the preparation methods of short Radix Sangusorbae extract, comprise the following steps：Take short garden burnet herb or root Portion smashes, and measures cold soaking 18-28h for 15-30 times with 85%-95% ethyl alcohol, collects filtrate, repeat 2-5 times, filtrate decompression is steamed It evaporating, concentrates filtrate, low temperature drying obtains crude extract, crude extract is decolourized through macroporous resin adsorption, after 85%-95% ethanol elutions, Concentration is evaporated, frozen drying,
Compared with prior art, the present invention possesses following excellent benefit：The present invention is by concentrating short garden burnet plant Extraction obtains Objective extraction object, and cytotoxicity experiment and cosmetics for skin activity experiment have been carried out to Objective extraction object, it was demonstrated that The security of Objective extraction object specifies the excellent cosmetic active of Objective extraction object and pharmaceutical activity.The present invention improves short The utilization function and added value of garden burnet, and expanded the source of natural skin care product plant material.The present invention is short by testing extraction The activity extract of garden burnet herb, and cytotoxicity and cosmetics for skin activity experiment have been carried out, specify short Radix Sangusorbae extract Not only possess good security and also show good antioxidation activity, it is former to have made good natural skin care product plant Material breaches the limitation of short garden burnet tradition Land use systems, has widened the utilization ways of short garden burnet, for improving the money of short garden burnet Source utility value is of great significance.
Description of the drawings：
Fig. 1：Short garden burnet herb or root extract are to the scavenging capacity of DPPH free radicals；
Fig. 2：The preparation technology flow chart of short Radix Sangusorbae extract.
Specific embodiment：
Below in conjunction with attached drawing, the present invention is described in more detail with the embodiment of the present invention, but is not intended to limit the present invention Scope.
Embodiment 1：
The preparation of short Radix Sangusorbae extract：
Short garden burnet (Sanguisorba filiformis (Hook.f.) Hand.-Mazz.) herb sample 12g is weighed, it will Sample grind into fine powder, is then collected filtrate with Buchner funnel, is repeated with 85% ethyl alcohol, 25 times of amount (361-362mL) cold soakings for 24 hours 3 times.Filtrate with Rotary Evaporators is evaporated under reduced pressure, concentrates filtrate, filtrate low temperature drying is concentrated, obtains crude extract.Crude extract is passed through Macroreticular resin (D101) adsorption bleaching after 95% ethanol elution, eluent concentration is evaporated, frozen drying obtains short garden burnet Herb extract 3.3g, recovery rate 27.5%.
Alternatively, weigh short garden burnet (Sanguisorba filiformis (Hook.f.) Hand.-Mazz.) root samples 12g by sample grind into fine powder, with 95% ethyl alcohol, 20 times of amount cold soaking 20h, then collects filtrate with Buchner funnel, is repeated 4 times. Filtrate with Rotary Evaporators is evaporated under reduced pressure, concentrates filtrate, filtrate low temperature drying is concentrated, obtains crude extract.By crude extract through macropore Resin (D101) adsorption bleaching after 90% ethanol elution, eluent concentration is evaporated, frozen drying obtains short garden burnet herb Extract 3.2g, recovery rate 26.7%；
Embodiment 2：
The cytotoxicity experiment of short Radix Sangusorbae extract：By MTS colorimetric methods, the CC of sample is determined50(50%cytotoxic Drug concentration when concentration), i.e., to 50% cell generation toxicity, so that it is determined that the safety of following activity experiment Sample concentration.
Experimental method：In 96 porocyte culture plates, B16 cells are mixed with the drug solution to be measured of various concentration, setting 3 A repeating hole, while the blank control of not drug containing is set, 37 DEG C, 5%CO2It cultivates for 24 hours, using MTS colorimetric determination cells Toxicity, microplate reader measure OD values, measure wavelength 490nm.CC is calculated50Value.
Cell survival rate (%)=experimental port OD490nm/ blank well OD490nm× 100%
Experimental result：The cell survival rate of short garden burnet herb extract is 87.343%, belongs to normal level, can substantially see Go out short Radix Sangusorbae extract does not have toxic action to cell, and the security of the natural material as cosmetics for skin is guaranteed 's.
Embodiment 3：
DPPH radicals scavengings are tested：By the short garden burnet herb of drug to be measured or root extract and DPPH (final concentration of 100 μM) hybrid reaction, 3 repeating holes are set, while the not blank control of drug containing and Trolox positive controls are set, 30 DEG C, 1h, Microplate reader measures OD values, Detection wavelength 515nm.Antioxygen rate is calculated.
Antioxygen rate (%)=(1- experimental ports OD515nm/ blank well OD515nm) × 100%
Experimental result：In an experiment, short garden burnet herb or root extract show significant DPPH radical scavenging activities Power, and according to concentration gradient, good dose-effect relationship (see Fig. 1) is presented, is used as possessing crease-resistant or sun-proof potentiality makeup The good natural botanical source of product activity.
The present invention provides the topical composition with natural plant composition or natural plant composition mixture, it is pharmaceutically Or acceptable carrier on cosmetics.These compositions can inhibit the tyrosinase activity of skin, the generation suppression of B16 cells melanin System activity, elastin laminin activity, TNF-α secretion inhibitory activity promote collagen secretion activity, inhibit the oxidation of skin.These Composition can be used for microgroove and/wrinkle, skin erythema, dryness, sensitive skin, pruitus, colour of skin unevenness or skin blackspot, skin Skin inflammation and other skin uncomfortable diseases related to this.These compositions include the extract that is obtained from short garden burnet or from this The active ingredient obtained in plant.These compositions can further inhibit the lipoxidase of skin, and COX (Cycloxygenase) is living Property, MMP-1 and MMP-3 are active.In these compositions the content of short Radix Sangusorbae extract be the 0.001wt% of composition gross weight extremely 20.0wt%.These compositions can be shin moisturizer, face cream or emulsion etc..The extract of short garden burnet can be water-soluble extraction Object, the extract can use alcohol (such as methanol, ethyl alcohol, propyl alcohol, butanol etc.) to extract.
The composition containing short Radix Sangusorbae extract can be used for topical skin care or hair products in the present invention.These combinations Object can make emulsifier (such as oil-in-water, Water-In-Oil, silicon Water-In-Oil, water bag silicone oil, W/O/W, Water-In-Oil bag oil, oil Bag water bag silicone oil etc.), creme, shin moisturizer, liquid (such as aqua or water-alcohol solution), anhydrous substrate (such as lipstick or pulvis Deng), gel, ointment, milk, ointment, spray, solid formulation, eye cream etc..
Topical skin care compositions in the present invention include the extract of short garden burnet or are obtained from short Radix Sangusorbae extract Component or ingredient.These compositions of local application can mitigate appearance (such as erythema, allergic skin, the skin of erythema Skin redness, inflammation etc.), it can also mitigate the pain caused by erythema.Erythema generally can be by skin sun burn, the electricity of skin Treatment, skin burn, Contact hyper sensitization, systemic anaphylaxis, dermal toxicity, exercise, insect bite, bacterium infection, virus infection, fungi sense Dye, protozoa infection, massage and dermatitis caused by blowing etc..
Topical skin care compositions in the present invention include the extract of short garden burnet or are obtained from short Radix Sangusorbae extract Component or ingredient.These compositions of local application can treat dry skin, sensitive skin or skin itching or reduce colour of skin unevenness Appearance.
Topical skin care compositions in the present invention include the extract of short garden burnet or are obtained from short Radix Sangusorbae extract Component or ingredient obtain.These compositions of local application can reduce the appearance of microgroove or wrinkle.These extracts can be water-soluble Property or water-insoluble extract, they can be extracted with alcohol (methanol, ethyl alcohol, propyl alcohol or butanol etc.), glycol, oil, water etc..
Topical skin care compositions in the present invention include the extract of short garden burnet or are obtained from short Radix Sangusorbae extract Component or ingredient.These compositions of local application can treat or prevent skin disease.Skin disease include dry skin, skin itching, Chafing, erythema, skin allergy, pruritus, Aranea blood vessel, freckle, senile plaque expelling, senile purpura, seborrheic keratosis, black spot, Pimple, microgroove or wrinkle, tubercle, skin sun-damaged, dermatitis (seborrhea, nummular dermatitis, contact dermatitis, dystopy Property dermatitis, exfoliative dermatitis, Perioral Dermatitis and stasis dermatitis etc.), psoriasis, epifolliculitis, rosacea, acne, purulence born of the same parents disease, Erysipelas, erythrasma, eczema, sun burn, skin burn, open wound, infective factors etc..Skin disease can also be by being exposed to Under ultraviolet light, the age, radiate, be chronically exposed under the sun, environmental pollution, air pollution, wind, hot and cold, chemicals, pathology, suction Cigarette or shortage nutrition etc. cause.This method increases the conversion of keratoderma speed by the composition of the local application present invention Degree increases the synthesis of collagen in fibroblast, enhances cellular anti-oxidant defense mechanism (the oxidation resistant enhancing energy of such as outer layer Improve, supplement or prevent cellular antioxidants such as Skin Cell --- keratinocyte, melanocyte, Langerhans cell It is middle reduction or prevent skin, cell, albumen and lipid oxidation damage catalyzing enzyme and glutathione loss), inhibit black The generation of melanin in plain cell, reduce or prevent skin oxidative damage (including reduce lipid peroxidation enzyme quantity and/or The oxidation of albumen in skin).
Cosmetic composition of the present invention can reduce the quantity of internal oxidation and/or external oxidation damage in cell, increase The synthesis of collagen in cell reduces the generation of the infection cell factor in cell.These cells are formed including people's epidermal keratinocytes Cell, fibroblasts of adult human dermis or human melanoma cell or their any combination (such as Human keratinocytes and people into The combination of fibrocyte or the combination of people's keratinocyte and human melanoma cell).
Cosmetic composition of the present invention can treat hyperpigmentation, and this method can also include and determine whether a people needs It is excessively calm to treat skin pigment.By application the present invention in composition can be additionally used in reduce skin appearance in senile plaque expelling, Hypopigmentation of skin, freckle reduce or prevent the microgroove or wrinkle of skin, increase the tightness of skin.
Composition of the present invention containing short Radix Sangusorbae extract has anti-oxidant, inhibition tyrosinase, inhibits pancreatopeptidase E, inhibits TNF-α enzyme, the ability for inhibiting lipoxidase, inhibiting COX-1 and/or COX-2 enzymes and inhibiting the activity of MMP-1 and/or MMP-2. These compositions can be used as topical skin composition, including acceptable carrier in dermatology.These compositions can treat or in advance It is anti-with tyrosinase in skin oxidative, Skin Cell, pancreatopeptidase E, TNF-α enzyme, lipoxidase, COX-1 and/or COX-2 enzymes and The relevant skin disease of activity of MMP-1 and/or MMP-2.
Embodiment 4：
Short Radix Sangusorbae extract is made by embodiment 1, by extract and excipient weight than 1:1 ratio adds in excipient, Pelletizing press sheet.
Embodiment 5：
Short garden burnet activity extract is made by embodiment 1, by extract and excipient weight than 1:2 ratio adds in figuration Agent, pelletizing press sheet.
Embodiment 6：
Short Radix Sangusorbae extract is made by embodiment 1, routinely capsule is made in capsule preparations method.
Embodiment 7：
Short Radix Sangusorbae extract first is made by embodiment 1, then tablet is made as follows
Tablet：Short Radix Sangusorbae extract 100mg
Appropriate starch
Appropriate corn pulp
Magnesium Stearate proper quantity
Embodiment 8：
Capsule：Short Radix Sangusorbae extract 100mg
Appropriate starch
Magnesium Stearate proper quantity
Preparation method：Red short Radix Sangusorbae extract with auxiliary agent is mixed, sieves, is uniformly mixed in suitable container, The mixture arrived is packed into hard gelatin capsule.
Embodiment 9：
Preparation method：A kind of ingredient is added under stirring every time in the double distilled water of proper volume, until completely deep solve, so After add another ingredient.After adding water to 2ml, which on sterilizing filter is filtered, is fitted into bottle and according to appropriate Dosage separate.
Embodiment 10：
Dripping pill：Short Radix Sangusorbae extract 1g
Macrogol 6000 9g
Preparation method：The preparation of short 6000 molten liquid of Radix Sangusorbae extract：Short Radix Sangusorbae extract is weighed by above-mentioned recipe quantity, is added in suitable Absolute ethyl alcohol is measured, (60 DEG C of water-bath heat preservations) after low-grade fever dissolving, is added in the polyethylene glycol molten liquid of recipe quantity, is stirred It is even, until ethyl alcohol is waved to the greatest extent, it is statically placed in 60 DEG C of water-baths and keeps the temperature 30 minutes, treat that bubble eliminates, then will eliminate the upper of bubble Mixing molten liquid is stated to be transferred in surge drum, under conditions of 80-85 DEG C of heat preservation, control drop speed is instilled in condensate liquid dropwise, It waits that condensations are complete, and incline condensate liquid, collects dripping pill, drip is net and removes the condensate liquid on ball with filter paper, places in silica gel drier Or it spontaneously dries.
Embodiment 11：
Tablet：Embodiment 1 gained short Radix Sangusorbae extract 10mg, lactose 180mg, starch 55mg, magnesium stearate 5mg；
Preparation method：Extract, newborn sugar and starch are mixed, uniformly moistened with water, the mixture sieving after moistening simultaneously Dry, re-sieving adds in magnesium stearate, then by mixture tabletting, every weight 250mg, compounds content 10mg.
Embodiment 12：
Ampulla：Embodiment 1 gained short Radix Sangusorbae extract 2mg, sodium chloride 10mg；
Preparation method：Short Radix Sangusorbae extract and sodium chloride are dissolved in suitable water for injection, filter acquired solution, It is fitted under aseptic condition in ampoule bottle.
Embodiment 13：
Capsule：Embodiment 1 gained short Radix Sangusorbae extract 10mg, lactose 187mg, magnesium stearate 3mg；
Preparation method：Short Radix Sangusorbae extract with auxiliary agent is mixed, is sieved, is uniformly mixed, obtained mixture is packed into hard Gelatine capsule, each capsule weight 200mg, active component content 10mg.
Embodiment 14：
Skin whitener formula (W%) containing short Radix Sangusorbae extract：
Deionized water adds to 100
The cosmetics of the above-mentioned formula of the present invention are made in the method for routinely making cosmetics.
Embodiment 15：
Emulsion formulations (W%) containing short Radix Sangusorbae extract：
Deionized water adds to 100
The cosmetics of the above-mentioned formula of the present invention are made in the method for routinely making cosmetics.
Embodiment 16：
Formula：
A oil phases w (mass percent)
Beeswax 2%, stearic acid 5%, octadecyl alcolol 3%, lanolin 2%, sorbester p17 3%, POLYSORBATE 80 3%, paraffin oil 3%.
B water phase w (mass percent)
Glycerine 5%, the short Radix Sangusorbae extract 5% of the present invention, triethanolamine 0.3%, distilled water 75%.
C essence is appropriate, propylben 0.2%
Method：
Oil phase is placed in thermostat water bath be heated with stirring to 90 DEG C be completely melt into it is homogeneous after, be slowly added to water phase, after Continuous stirring 30min；Slow cooling continues to stir to essence, propylben is added at 45 DEG C；Treat that temperature is down to 35 DEG C or so When, stop stirring, stand, cooling obtains newborn creamy product.

Claims (8)

1. short Radix Sangusorbae extract, is prepared by following methods：Short garden burnet herb or root are taken, is smashed, with short garden burnet The 15-30 times of 85%-95% ethyl alcohol measured, cold soaking 18-28h collect filtrate, repeat 2-5 times, and filtrate decompression distillation concentrates filtrate, Low temperature drying obtains crude extract, and by crude extract through macroreticular resin D101 adsorption bleachings, after 85%-95% ethanol elutions, concentration is steamed It is dry, frozen drying.
2. cosmetics are made of short Radix Sangusorbae extract described in claim 1 and cosmetics common carrier, the carrier is one Kind or a variety of Aquo Systems.
3. cosmetics according to claim 2, it is characterised in that one or more Aquo Systems are C1-4 alcohol, fat Fat alcohol, polyalcohol, aliphatic ether, fatty ester, vegetable oil, mineral oil, liposome, laminar lipid materials, silicone oil, water or they Combination.
4. cosmetics according to claim 3, it is characterised in that the polyalcohol is ethylene glycol, propylene glycol.
5. application of the short Radix Sangusorbae extract described in claim 1 in cosmetics are prepared.
6. short Radix Sangusorbae extract described in claim 1 prepare that tender skin is crease-resistant or sun-proof or skin-lightening cosmetic in application.
7. application of the short Radix Sangusorbae extract described in claim 1 in antioxidant is prepared.
8. the preparation method of short Radix Sangusorbae extract, comprises the following steps：Short garden burnet herb or root are taken, is smashed, with short The 85%-95% ethyl alcohol cold soaking 18-28h that 15-30 times of elm is measured collect filtrate, repeat 2-5 times, filtrate decompression distillation, concentration filter Liquid, low temperature drying obtain crude extract, by crude extract through macroreticular resin D101 adsorption bleachings, after 85%-95% ethanol elutions, and concentration It is evaporated, frozen drying.
CN201510728084.5A 2015-10-30 2015-10-30 Short Radix Sangusorbae extract and its application in cosmetics are prepared Active CN105193680B (en)

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CN108685802B (en) * 2018-08-07 2019-10-18 广东丸美生物技术股份有限公司 Anticorrosive composite and its preparation method and application
CN109288781A (en) * 2018-09-03 2019-02-01 芜湖凌梦电子商务有限公司 Whitening and softening skin essence cream and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63303910A (en) * 1987-06-03 1988-12-12 Kanebo Ltd Beautifying cosmetic
CN103768385A (en) * 2012-10-18 2014-05-07 曾样军 Medicinal liquor capable of maintaining beauty and keeping young and preparation method thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS63303910A (en) * 1987-06-03 1988-12-12 Kanebo Ltd Beautifying cosmetic
CN103768385A (en) * 2012-10-18 2014-05-07 曾样军 Medicinal liquor capable of maintaining beauty and keeping young and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
《地榆提取物抗氧化与抗过敏作用研究》;魏智芸等;《时珍国医国药》;20090831;第20卷(第8期);第1958-1960页，尤其是第1959页"2.1提取分离方法""表1" *

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