JP2012111727A - Cell activator - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a cell activator having excellent cell activation action.SOLUTION: The cell activator contains a seed or its extract of Salvia hispanica which is a labiatae plant.

Description

  The present invention relates to a plant-derived cell activator.

  Aging is progressing in the organs of the whole body, but among the skin that can be seen with the eyes, especially on the face where the consciousness is easily concentrated, wrinkles and fine wrinkles that occur with aging, sagging, spots, etc. A lot of middle-aged and older people, especially women. Recently, the desire to be seen younger not only in women but also in men has become stronger, and it is desired to alleviate the skin sensation phenomenon that appears with aging. In view of this, it has been screamed that there is a need to prevent or improve wrinkles, sagging, spots, etc. by keeping the appearance young or alleviating the aging phenomenon.

There are various causes of skin sensation and functional decline, but one factor is the involvement of fibroblasts. Fibroblasts are cells that exist in the dermis and produce collagen, elastin, hyaluronic acid, etc., and are known to contribute to forming a dermal structure by using collagen as a fiber bundle. If these fibroblasts are damaged or decreased by aging or UV exposure, regular fibers cannot be generated, and skin aging such as wrinkles and sagging occurs.
Therefore, the component having an activating effect on the fibroblasts is useful as an active component of the anti-aging agent.
Based on these findings, various components having a cell activating effect, particularly on the skin, are aimed at maintaining or maintaining the skin function and delaying or improving the aging phenomenon by promoting and activating the proliferation of fibroblasts. In anticipation of calmness, ingredients derived from natural products such as plants have been proposed.

For example, Patent Document 1 describes a cell activator comprising a primrose family primula plant extract as an active ingredient, and this ingredient also has an antioxidant action, and is a skin caused by external stress such as aging and ultraviolet rays. It is said that the aging symptoms can be effectively prevented and improved.
Patent Document 2 describes a fibroblast activator containing fruit extracts such as mangosteen and mango, and is said to be effective in preventing skin aging and preventing or improving rough skin.
Patent Document 3 describes a fibroblast activator containing a water-soluble saccharide in which two or more saccharides are β1-4 bonded.
Patent Document 4 describes a cell activator containing a Bruguiera plant extract as an active ingredient.
Patent document 5 describes a cell activator characterized by containing an extract of the Thydium bipinnatulum genus Thuidium bipinnatulum.

Japanese Patent No. 3507055 (Japanese Patent Laid-Open No. 2003-252740) JP 2006-249051 JP2009-67758 Japanese Patent No. 4245862 Japanese Patent No. 4567306

However, in the inventions described in Patent Documents 1 to 5, since any active ingredient is water-soluble, there is a difficulty in effectively penetrating from the skin surface to the dermis, and the market for fibroblast activation is high. In order to respond to the demand, a substance exhibiting a superior fibroblast activation action has been desired.
The present invention has been made in response to such a conventional situation, and an object thereof is to provide a new plant-derived cell activator.

  In view of the current situation, the present inventors have conducted extensive research, and as a result, have found that a specific plant has an excellent cell activating effect, and is particularly useful as a cell activating agent, and completes the present invention. It came.

  The present invention is a cell activator comprising a species of Salvia hispanica which is a Labiatae plant or an extract thereof.

  The cell activator of the present invention activates fibroblasts and promotes the production of extracellular matrix such as collagen and elastin, and can prevent / suppress skin aging including wrinkles and repair skin damage. is there. In addition, since ingredients derived from natural products are used, it is safe even for internal use or external application to humans or animals.

It is a figure which shows the fibroblast activation degree (%) when the ethyl acetate extract of a chia seed is used. It is a figure which shows the fibroblast activation degree (%) when the acetone extract of a chia seed is used. It is a figure which shows the fibroblast activation degree (%) when the chloroform extract of a chia seed is used. It is a figure which shows the fibroblast activation degree (%) when using the hexane extract of a chia seed. It is a figure which shows the fibroblast activation degree (%) when the methanol extract of a chia seed is used. It is a figure which shows the fibroblast activation degree (%) when the ethanol extract of a chia seed is used.

Hereinafter, the present invention will be described in detail.
The cell activator of the present invention was found to have excellent fibroblast proliferation promoting ability in a cell activation test using dermal fibroblasts. The cell activator of the present invention is suitable as a material for pharmaceuticals (for example, external preparations for skin and internal preparations), quasi drugs, and cosmetics. Moreover, it is applicable also to food-drinks (for example, a supplement and a drink).

  Salvia hispanica (Salvia hispanica) used in the present invention is a mint plant belonging to the genus Salvia and is also called a chia, and its species is called a chia seed. In the present invention, a species of Salvia hispanica or an extract thereof is used, and in particular, a thia oil obtained from the species is preferable.

About Salvia hispanica, the antioxidant composition containing the oil (thia oil) derived from the seed | species (thia seed) and the polycationic casein phosphopeptide complex is known (international publication WO2005 / 084452). .
However, this antioxidant composition is a composition for reducing the oxidative reaction that occurs in foods, cosmetics, etc. that form an emulsion and enhancing the stability, and does not directly lead to cell activation. . There has never been a report that a species of Salvia hispanica or an extract thereof has a cell activating effect, and it was first discovered by the present inventors.

  In addition, the species of Salvia / Hispanica used in the present invention or an extract thereof is a product obtained by squeezing Salvia / Hispanica seeds as it is or dried to make a thia oil, or as it is or after squeezing, or after drying these , Refers to those extracted with a solvent.

  In the case of an extract, the extraction solvent used for extraction may be any solvent other than water as long as it is used for normal extraction, but in particular, ethyl acetate, acetone, chloroform, hexane, methanol, ethanol, hydrous ethanol, alone or in combination. Are preferably used. The solvent is preferably used at a temperature between room temperature and the boiling point of the solvent. Furthermore, supercritical extraction, or a component obtained by distillation, activated carbon treatment, steam distillation or a combination of these may be used. Moreover, you may use the obtained component by converting into another medium (the above-mentioned solvent, powders, such as calcium silicate), liposome preparations, such as a lecithin, and encapsulating.

  The cell activator of the present invention is preferably composed of Salvia / Hispanica seeds or an extract thereof (dry mass), but may contain other various components as long as the effects of the present invention are not impaired. .

  The component derived from the species of Salvia / Hispanica according to the present invention has an excellent fibroblast activation effect as described later. Therefore, the present invention is preferably used as an anti-aging skin external preparation containing, as an active ingredient, a component derived from the species of Salvia / Hispanica.

  When the cell activator in the present invention is included in the anti-aging skin external preparation, the content of the cell activator is preferably 0.0001 to 20% by mass, more preferably 0.001 to 10% by mass. It is.

The anti-aging skin external preparation can be produced according to a conventional method.
As an ingredient constituting an anti-aging skin external preparation, other ingredients used in skin external preparations such as cosmetics and pharmaceuticals including quasi-drugs are usually used as needed, as long as the effects of the present invention are not impaired. Can be appropriately blended. Furthermore, by preparing the above-mentioned cell activator in combination with one or more other medicinal agents, the cell activation effect is further enhanced, or a skin external preparation that exhibits other medicinal effects together with the cell activation effect is prepared. Can do. Examples of other medicinal agents include, but are not limited to, whitening agents, UV protection agents, antibacterial agents, anti-inflammatory agents, other cell activators, active oxygen scavengers, and moisturizers. Absent.

  Moreover, arbitrary components other than the cell activator of this invention can be mix | blended with the said skin external preparation for anti-aging. Examples of such components include, but are not limited to, physiologically active substances such as amino acids, lipids, sugars, hormones, enzymes, and nucleic acids. In addition, components that are usually used in the production of cosmetics, quasi-drugs, skin external preparations and the like, for example, water (purified water, hot spring water, deep water, etc.), oil agents, Surfactant, metal soap, gelling agent, powder, alcohol, water-soluble polymer, film-forming agent, resin, inclusion compound, fragrance, deodorant, salt, pH adjuster, refreshing agent, plant / animal -Extracts derived from microorganisms, blood circulation promoters, astringents, antiseborrheic agents, chelating agents, keratolytic agents, enzymes, hormones, other vitamins and the like can be used as necessary.

  Others, disodium edetate, trisodium edetate, sodium citrate, sodium polyphosphate, sodium metaphosphate, sequestering agents such as gluconic acid, caffeine, tannin, verapamil, tranexamic acid and its derivatives, licorice extract, grabrizine Hot water extract of fire spine fruit, various herbal medicines, drugs such as tocopherol acetate, glycyrrhizic acid and its derivatives or salts thereof, vitamin C, magnesium ascorbate phosphate, glucoside ascorbate, arbutin, kojic acid, alkoxysalicylic acid and / Or other whitening agents such as salts thereof, saccharides such as glucose, fructose, mannose, sucrose, trehalose and the like can be appropriately blended.

The anti-aging skin external preparation can be applied to the skin to prevent wrinkle formation and / or in a cosmetic method for reducing or eliminating the formed wrinkles. The usage and dosage of the anti-aging skin external composition in such a cosmetic method are not particularly limited and are appropriately determined depending on the dosage form and the wrinkle state of the skin to be treated, but typically several times per day For example, 1 to 5 times, an appropriate amount, for example, 0.1 ml to 1 ml per 1 cm ² , can be directly rubbed into the skin, or an appropriate amount can be soaked in gauze and the like, and then applied to the skin.

  The dosage form of the anti-aging external preparation for skin is not particularly limited as long as it can exert the effects of the present invention. For example, solids such as soap and lipstick, solution systems, solubilization systems, emulsification systems, Arbitrary dosage forms such as powder dispersion, water-oil two-layer system, water-oil-powder three-layer system, ointment, gel, and aerosol can be used. However, it is not limited to these.

  The anti-aging skin external preparation is applied, for example, in the field of cosmetics and pharmaceuticals including quasi-drugs, and examples of product forms include ointments, creams, emulsions, lotions, essences, jellies, gels, and packs. , Masks, foundations, microneedles, etc.

  When the cell activator of the present invention is blended in foods and drinks, feeds, etc., the blending amount (dry mass) of the plant body or its extract is appropriately determined according to the type, purpose, form, usage method, etc. For example, in the case of tablets or capsules in the total amount of food and drink, 1 to 90% by mass is preferable, and in other foods and drinks, 0.001 to 50% by mass is preferable. It is preferable to prepare so that the daily intake of the plant or its extract per adult is about 1 to 10 g. In particular, when used as a health food or drink, it is preferable to contain the active ingredient of the present invention in such an amount that a predetermined effect is sufficiently exhibited.

  As a form of food-drinks or feed, it can be arbitrarily shaped into, for example, granules, granules, pastes, gels, solids, or liquids. These include various known substances that are recognized to be contained in foods and drinks, such as binders, disintegrants, thickeners, dispersants, reabsorption accelerators, flavoring agents, buffers, and surfactants. Excipients such as solubilizers, preservatives, emulsifiers, tonicity agents, stabilizers and pH adjusters may be included as appropriate.

  When the cell activator of the present invention is used as a pharmaceutical preparation, the preparation is suitably used orally or parenterally (intravenous administration, intraperitoneal administration, etc.). The dosage form is also arbitrary, for example, oral solid preparations such as tablets, granules, powders and capsules, oral liquid preparations such as internal liquids and syrups, and parenteral liquid preparations such as injections. These forms can also be appropriately prepared by known methods. For these pharmaceutical preparations, commonly used binders, disintegrants, thickeners, dispersants, reabsorption accelerators, corrigents, buffers, surfactants, solubilizers, preservatives, emulsifiers, isotonicity Excipients such as agents, stabilizers and pH adjusters may be used as appropriate.

When the cell activator of the present invention is used as an external preparation for skin, foods and drinks, pharmaceutical preparations, etc., it prevents / suppresses skin aging caused by a decrease in the function of proliferating fibroblasts, sagging / wrinkles skin, firmness / elasticity It is suitably used for the prevention and treatment of symptoms and diseases such as a decrease in blood pressure. When added to a food, it is expected that the anti-aging effect and the fibroblast activation effect will be exhibited from the inside by ingesting the food. In addition, the fibroblast activation action is promoted to help repair skin damage.
In addition, with the concept of physiological functions such as treatment, prevention, and improvement of the above symptoms and pathological conditions, it can be applied to external preparations for skin, functional foods and beverages, foods for specified health use, and the like.

  The method of adding the cell activator of the present invention to the external preparation for skin or food and drink may be added in advance or during the production, and may be appropriately selected in consideration of workability. .

The present invention will be described in more detail with reference to the following examples, but the present invention is not limited thereto. Unless otherwise specified, the amount is shown in mass%.
Initially, the preparation method of the plant extract used in the present Example, the test method regarding a fibroblast activation effect, and its result are demonstrated.

1. Preparation of sample (1) Preparation of ethyl acetate extract of chia seeds 200 mL of ethyl acetate was added to 20.024 g of the chia seeds pressed product, and immersed for 7 days at room temperature, followed by filtration. The solvent of the filtrate was distilled off to obtain 5.43 g (yield 27.1% by mass) of an oily non-volatile extract.
(2) Preparation of Chia Seed Acetone Extract 200 ml of acetone was added to 20.16 g of the chia seed pressed product, and the mixture was immersed for 7 days at room temperature and then filtered. The solvent of the filtrate was distilled off to obtain 5.63 g (yield 28.2% by mass) of an oily nonvolatile extract.
(3) Preparation of Chia Seed Chloroform Extract 180 ml of chloroform was added to 18.851 g of the chia seed compressed product, and after immersion for 7 days at room temperature, it was filtered. The solvent of the filtrate was distilled off to obtain 4.78 g (yield 25.4% by mass) of an oily non-volatile extract.
(4) Preparation of hexane extract of chia seeds 200 mL of hexane was added to 20.19 g of the chia seeds squeezed product, soaked at room temperature for 7 days, and then filtered. The solvent of the filtrate was distilled off to obtain 5.120 g (yield 25.6% by mass) of an oily non-volatile extract.
(5) Preparation of methanol extract of chia seeds 200 mL of methanol was added to 20.074 g of the chia seeds pressed product, and after immersion for 7 days at room temperature, filtration was performed. The solvent of the filtrate was distilled off to obtain 2.023 g (yield 10.1% by mass) of an oily non-volatile extract.
(6) Preparation of Chia Seed Ethanol Extract 200 mL of ethanol was added to 20.051 g of the chia seed pressed product, immersed for 7 days at room temperature, and then filtered. The solvent of the filtrate was distilled off to obtain 3.70 g (yield 18.5% by mass) of an oily non-volatile extract.

2. Fibroblast activation test of extract (1) Test method Fibroblasts derived from human newborns were inoculated into Dulbecco's modified Eagle's MEM medium (DMEM, Nissui) containing 10% calf serum (FBS), followed by 24 It seed | inoculated to the hole plate and left still in the atmosphere of 37 degreeC and a carbon dioxide concentration of 5 vol%. After cell attachment, the medium was replaced with a medium containing low-concentration serum (0.5%) and cultured continuously for one day and night.
Then, using the extract prepared above, the sample preparation solution was added and mixed so that the sample concentrations were 0, 0.005, 0.01, and 0.1% by volume with respect to the medium. On the third day of the culture, the number of fibroblasts grown in each sample preparation solution was measured using an Alamar Blue solution, and the degree of fibroblast activation was evaluated using a sample to which no extract was added as a control.
At this time, cells cultured under the same conditions in Dulbecco's modified Eagle's MEM medium containing 5% calf serum (FBS) were used as a positive control (%) for growth.

(2) Evaluation results with Alamar Blue solution The medium grown by adding each specimen preparation solution was replaced with a fresh medium, and after culturing with the addition of one-tenth volume of Alamar Blue reagent, the excitation wavelength was 544 nm, fluorescence Fluorescence intensity was determined by measuring at a wavelength of 590 nm, and the degree of fibroblast activation was evaluated with 100% as the value to which no extract was added (only dimethyl sulfoxide solvent). The results are shown in FIGS.

FIG. 1 shows the degree of fibroblast activation (%) when an ethyl acetate extract of chia seeds was used. The positive control (%) was 212%.
FIG. 2 shows the degree of fibroblast activation (%) when an acetone extract of chia seed was used. The positive control (%) was 242%.
FIG. 3 shows the degree of fibroblast activation (%) when a chia seed chloroform extract was used. The positive control (%) was 225%.
FIG. 4 shows the degree of fibroblast activation (%) when using a hexane extract of chia seeds. The positive control (%) was 248%.
FIG. 5 shows the degree of fibroblast activation (%) when a chia seed methanol extract was used. The positive control (%) was 286%.
FIG. 6 shows the degree of fibroblast activation (%) when an ethanol extract of chia seed was used. The positive control (%) was 227%.

  As can be seen from FIGS. 1 to 6, it was confirmed that the extract from Salvia Hispanica seeds (chia seeds) according to the present invention has a high cell activation ability against human neonatal fibroblasts. . Therefore, the cell activator of the present invention exerts an extremely excellent action on skin fibroblasts when applied to the skin, and effectively prevents and improves skin wrinkles and sagging caused by aging, UV exposure, etc. can do. In addition, the chia seed extract has an excellent cell activation activity when any of the extraction solvents is used, and the optimum concentration varies depending on the type of the extraction solvent.

  The formulation example of the cell activator which concerns on this invention is shown below. All external preparations had excellent wrinkle prevention and improvement effects.

Formulation Example 1: Wrinkle prevention / improvement cream component Formulation amount (% by mass)
Stearic acid 5.0
Stearyl alcohol 4.0
Isopropyl myristate 18.0
Glycerol monostearate 3.0
Propylene glycol 10.0
This cell activator 0.01
(Chia seed hexane extract)
Retinol 0.05
Caustic potash 0.2
Sodium bisulfite 0.01
Preservative Appropriate amount of perfume Appropriate amount of ion-exchanged water

(Manufacturing method)
Propylene glycol and caustic potash were added to ion-exchanged water, dissolved, heated and kept at 70 ° C. (aqueous phase). The other ingredients were mixed, heated and melted, and kept at 70 ° C. (oil phase). The oil phase was gradually added to the aqueous phase, and after the addition was completed, the temperature was maintained for a while to cause the reaction. Thereafter, the mixture was uniformly emulsified with a homomixer and cooled to 30 ° C. while stirring well.

Formulation Example 2: Wrinkle prevention / improvement cream component Formulation amount (% by mass)
Stearic acid 6.0
Sorbitan monostearate ester 2.0
Polyoxyethylene (20 mol)
Sorbitan monostearate 1.5
Propylene glycol 10.0
This cell activator 0.1
(Chia seed ethyl acetate extract)
Carnosine 1.0
Glycerin trioctanoate 10.0
Squalene 5.0
Sodium bisulfite 0.01
Ethylparaben 0.3
Perfume Appropriate amount of ion-exchanged water

(Manufacturing method)
Propylene glycol is added to ion-exchanged water, dissolved, heated and maintained at 70 ° C. (aqueous phase). The other ingredients were mixed, heated and melted, and kept at 70 ° C. (oil phase). The oil phase was added to the aqueous phase, pre-emulsified, and uniformly emulsified with a homomixer, and then cooled to 30 ° C. while stirring well.

Formulation Example 3: Wrinkle prevention / improvement cream component Amount (% by mass)
Stearyl alcohol 7.0
Stearic acid 2.0
Hydrogenated Lanolin 2.0
Squalane 5.0
2-Octyldodecyl alcohol 6.0
Polyoxyethylene (25 mol) cetyl alcohol ether 3.0
Glycerin monostearate ester 2.0
Propylene glycol 5.0
This cell activator 1.0
(Chia seed acetone extract)
Perfume proper amount Sodium bisulfite 0.03
Ethylparaben 0.3
Ion exchange water

(Manufacturing method)
Propylene glycol was added to ion-exchanged water, dissolved, heated and kept at 70 ° C. (aqueous phase). The other ingredients were mixed, heated and melted, and kept at 70 ° C. (oil phase). The oil phase was added to the aqueous phase, preliminarily emulsified, and uniformly emulsified with a homomixer, and then cooled to 30 ° C. while stirring well.

Formulation Example 4: Wrinkle prevention / improvement emulsion component content (% by mass)
Stearic acid 2.5
Cetyl alcohol 1.5
Vaseline 5.0
Liquid paraffin 10.0
Polyoxyethylene (10 mol) monooleate 2.0
Polyethylene glycol 1500 3.0
Triethanolamine 1.0
This cell activator 0.005
(Chia seed methanol extract)
Retinol 0.005
Sodium bisulfite 0.01
Ethylparaben 0.3
Carboxyvinyl polymer 0.05
Perfume Appropriate amount of ion-exchanged water

(Manufacturing method)
Carboxyvinyl polymer was dissolved in a small amount of ion-exchanged water (A phase). Polyethylene glycol 1500 and triethanolamine were added to the remaining ion-exchanged water, dissolved by heating and kept at 70 ° C. (aqueous phase). The other ingredients were mixed, heated and melted, and kept at 70 ° C. (oil phase). The oil phase was added to the aqueous phase for preliminary emulsification, and the A phase was added, and the mixture was uniformly emulsified with a homomixer and cooled to 30 ° C. while stirring well after the emulsification.

Formulation Example 5: Wrinkle prevention / improvement emulsion component content (mass%)
(Oil phase)
Stearyl alcohol 1.5
Squalene 2.0
Vaseline 2.5
Deodorized liquid lanolin 1.5
Evening primrose oil 2.0
This cell activator 0.1
(Chiaseed ethanol extract)
Isopropyl myristate 5.0
Glycerol monooleate 2.0
Polyoxyethylene (60 mol) hydrogenated castor oil 2.0
Tocopherol acetate 0.05
Ethylparaben 0.2
Butylparaben 0.1
Ethylglycine 1.0
Perfume appropriate amount (water phase part)
Sodium bisulfite 0.01
Glycerin 5.0
Sodium hyaluronate 0.01
Carboxyvinyl polymer 0.2
Potassium hydroxide 0.2
Purified water residue

(Manufacturing method)
The oil phase part was dissolved at 70 ° C. The water phase part was dissolved at 70 ° C., the oil phase part was mixed with the water phase part, emulsified with an emulsifier and then cooled to 30 ° C. with a heat exchanger.

Formulation Example 6: Wrinkle prevention / improvement jelly component content (mass%)
95% ethyl alcohol 10.0
Dipropylene glycol 15.0
Polyoxyethylene (50 mol) oleyl alcohol ether 2.0
Carboxyvinyl polymer 1.0
Caustic soda 0.15
L-Arginine 0.1
This cell activator 0.001
(Chiaseed ethanol extract)
Methylparaben 0.2
Perfume Appropriate amount of ion-exchanged water

(Manufacturing method)
The carboxyvinyl polymer was uniformly dissolved in ion-exchanged water, while sarcosine and polyoxyethylene (50 mol) oleyl alcohol ether were dissolved in 95% ethanol and added to the aqueous phase. Subsequently, other components were added, and the mixture was neutralized with caustic soda and L-arginine to increase the viscosity.

Formulation Example 7: Wrinkle prevention / improvement cosmetic liquid ingredient content (% by mass)
(Phase A)
Ethanol (95%) 10.0
Polyoxyethylene (20 mol) octyldodecanol 1.0
This cell activator 1.0
(Chiaseed chloroform extract)
Methylparaben 0.15
Pantotenyl ethyl ether 0.1
(Phase B)
Potassium hydroxide 0.1
(Phase C)
Glycerin 5.0
Dipropylene glycol 10.0
Sodium bisulfite 0.03
Carboxyvinyl polymer 0.2
Purified water residue

(Manufacturing method)
The A phase and the C phase were uniformly dissolved, and the A phase was added to the C phase and solubilized. Subsequently, after adding B phase, it filled and manufactured.

Formulation Example 8: Wrinkle prevention / improvement pack component Amount (% by mass)
(Phase A)
Dipropylene glycol 5.0
Polyoxyethylene (60 mol) hydrogenated castor oil 5.0
(Phase B)
Olive oil 5.0
Tocophenol acetate 0.2
Ethylparaben 0.2
Fragrance 0.2
(Phase C)
Sodium bisulfite 0.03
This cell activator 0.1
(Chia seed ethyl acetate extract)
Polyvinyl alcohol (degree of saponification 90, degree of polymerization 2000) 13.0
Ethanol 7.0
Purified water residue

(Manufacturing method) A phase, B phase, and C phase were each dissolved uniformly, and B phase was added to A phase to solubilize. Subsequently, after adding this to the C phase, it filled and manufactured.

Formulation Example 9: Wrinkle prevention / improvement ointment component Amount (% by mass)
Polyoxyethylene (30 mol) cetyl ether 2.0
Glycerol monostearate 10.0
Liquid paraffin 10.0
Vaseline 40.0
Cetanol 6.0
Methylparaben 0.1
Butylparaben 0.1
Glycerin monostearate ester 2.0
This cell activator 5.0
(Chia seed acetone extract)
Propylene glycol 10.0
Ion exchange water Residual perfume appropriate amount

(Production Method) Propylene glycol was added to ion-exchanged water, dissolved, heated and kept at 70 ° C. (aqueous phase). Other components were mixed and dissolved at 70 ° C. (oil phase). An oil phase was added to the aqueous phase, and the mixture was uniformly emulsified with a homomixer, and filled after cooling.

Formulation Example 10: Wrinkle prevention / improvement cream component Amount (mass%)
Liquid paraffin 8.0
Vaseline 3.0
Dimethylpolysiloxane 2.0
Stearyl alcohol 3.0
Behenyl alcohol 2.0
Glycerin 5.0
Dipropylene glycol 4.0
Trehalose 1.0
Tetra-2-ethylhexanoic acid pentaerythrit 4.0
Polyisoethylene glyceryl monoisostearate 2.0
Polyoxyethylene glycerol monostearate 1.0
Lipophilic glyceryl monostearate 2.0
Citric acid 0.05
Sodium citrate 0.05
Potassium hydroxide 0.015
Oil-soluble licorice extract 0.1
Retinol palmitate (1 million units) 0.25
This cell activator 1.0
(Chia seed ethyl acetate extract)
Yeast extract 3.5
Tocopherol acetate 0.1
P-Hydroxybenzoate appropriate amount phenoxyethanol appropriate amount dibutylhydroxytoluene appropriate amount edetate trisodium 0.05
4-t-butyl-4′-methoxydibenzoylmethane 0.01
2-Ethylhexyl paramethoxycinnamate 0.1
β-carotene 0.01
Polyvinyl alcohol 0.5
Hydroxyethyl cellulose 0.5
Carboxyvinyl polymer 0.05
Purified water Residual fragrance

Formulation Example 11 Wrinkle Prevention / Improvement Cream Ingredient Amount (% by mass)
Vaseline 2.0
Dimethylpolysiloxane 2.0
Ethanol 5.0
Behenyl alcohol 0.5
Batyl alcohol 0.2
Glycerin 7.0
1,3-butylene glycol 5.0
Polyethylene glycol 20000 0.5
Jojoba oil 3.0
Squalane 2.0
Phytosteryl hydroxystearate 0.5
Tetra-2-ethylhexanoic acid pentaerythrit 1.0
Polyoxyethylene hydrogenated castor oil 1.0
Potassium hydroxide 0.1
Sodium pyrosulfite 0.01
Sodium hexametaphosphate 0.05
Stearyl glycyrrhetinate 0.1
Pantothenyl ethyl ether 0.1
Mishima Psycho Root Extract 2.0
Tranexamic acid methylamide hydrochloride 11.0
This cell activator 1.0
(Chia seed ethyl acetate extract)
Carnosine 3.5
Tocopherol acetate 0.1
Sodium hyaluronate 0.05
P-Hydroxybenzoate appropriate amount trisodium edetate 0.05
4-t-butyl-4'-methoxydibenzoylmethane 0.1
Diparamethoxycinnamic acid mono-2-ethylhexanoate glyceryl 0.1
Yellow iron oxide xanthane gum 0.1
Carboxyvinyl polymer 0.2
Purified water residue

Formulation Example 12: Wrinkle prevention / improvement cream ingredient content (mass%)
Vaseline 2.0
Dimethylpolysiloxane 2.0
Ethanol 5.0
Behenyl alcohol 0.5
Batyl alcohol 0.2
Glycerin 7.0
1,3-butylene glycol 5.0
Polyethylene glycol 20000 0.5
Jojoba oil 3.0
Squalane 2.0
Phytosteryl hydroxystearate 0.5
Tetra-2-ethylhexanoic acid pentaerythrit 1.0
Polyoxyethylene hydrogenated castor oil 1.0
Potassium hydroxide 0.1
Sodium pyrosulfite 0.01
Sodium hexametaphosphate 0.05
Stearyl glycyrrhetinate 0.1
Pantothenyl ethyl ether 0.1
Winged bean extract 1.0
Tranexamic acid methylamide hydrochloride 11.0
This cell activator 0.01
(Chia seed hexane extract)
Tocopherol acetate 0.1
Sodium hyaluronate 0.05
P-Hydroxybenzoate appropriate amount trisodium edetate 0.05
4-t-butyl-4'-methoxydibenzoylmethane 0.1
Diparamethoxycinnamic acid mono-2-ethylhexanoate glyceryl 0.1
Yellow iron oxide xanthane gum 0.1
Carboxyvinyl polymer 0.2
Purified water residue

Formulation Example 11 Wrinkle Prevention / Improvement Cream Ingredient Amount (% by mass)
Vaseline 2.0
Dimethylpolysiloxane 2.0
Ethanol 5.0
Behenyl alcohol 0.5
Batyl alcohol 0.2
Glycerin 7.0
1,3-butylene glycol 5.0
Polyethylene glycol 20000 0.5
Jojoba oil 3.0
Squalane 2.0
Phytosteryl hydroxystearate 0.5
Tetra-2-ethylhexanoic acid pentaerythrit 1.0
Polyoxyethylene hydrogenated castor oil 1.0
Potassium hydroxide 0.1
Sodium pyrosulfite 0.01
Sodium hexametaphosphate 0.05
Stearyl glycyrrhetinate 0.1
Pantothenyl ethyl ether 0.1
4-Methoxybenzoic acid 3.0
Tranexamic acid methylamide hydrochloride 11.0
This cell activator 0.03
(Chiaseed chloroform extract)
Carnosine 3.5
Tocopherol acetate 0.1
Sodium hyaluronate 0.05
P-Hydroxybenzoate appropriate amount trisodium edetate 0.05
4-t-butyl-4'-methoxydibenzoylmethane 0.1
Diparamethoxycinnamic acid mono-2-ethylhexanoate glyceryl 0.1
Yellow iron oxide xanthane gum 0.1
Carboxyvinyl polymer 0.2
Purified water residue

Formulation Example 14: Microneedle containing cell activation extract A production method according to the method described in JP-A-2005-152180, which is based on the following composition.
Maltose 94.9% by mass
Dextran 5.0
This cell activator 0.1
(Chiaseed chloroform extract)
The obtained microneedle was a microneedle having a high effect of enhancing skin firmness and elasticity, and having a high effect of preventing and improving wrinkles.

Formulation Example 15: Tablet 3000 mg / day (6 tablets)
Sucrose ester 200 6.7 (% by mass)
Crystalline cellulose 200 6.7
Methylcellulose 100 3.3
Glycerin 60 2.0
Hyaluronic acid 400 13.3
Vitamin E 60 2.0
Vitamin B6 40 1.3
α-Lipoic acid 40 1.3
This cell activator 1200 40.0
(Chiaseed ethanol extract)
Ceramide (konjac extract) 100 3.3
L-proline 600 20.0

Formulation Example 16: Soft capsule 3000 mg / day (6 tablets)
This cell activator 1060 35.3 (mass%)
(Chiaseed ethanol extract)
Eucommia extract 100 3.3
Carrot extract 100 3.3
Royal Jelly 100 3.3
Maca 60 2.0
GABA 60 2.0
Beeslow 120 4.0
Gelatin 950 31.7
Glycerol 240 8.0
Glycerin fatty acid ester 210 7.0

Formulation Example 17: Soft capsule 3000 mg / day (6 tablets)
This cell activator 1318 43.9 (mass%)
(Chiaseed ethanol extract)
Tocotrienol 1000 33.3
Resveratrol 2 0.1
Lotus germ extract 200 6.7
Elastin 360 12.0
DNA 60 2.0
Folic acid 60 2.0

Formulation Example 18: Drink 50 mL / day Eucommia extract 0.032% by mass
Carrot extract 0.032
Grape Seed Extract 0.032
Reduced maltose starch syrup 0.56
Erythritol 0.16
Citric acid 0.04
Perfume 0.026
N-acetylglucosamine 0.02
Hyaluronic acid 0.01
Vitamin E 0.006
Vitamin B6 0.004
Vitamin B2 0.002
α-Lipoic acid 0.004
This cell activator (equivalent to 3 g) 0.06
(Chia seed hydrous ethanol extract)
Ceramide (konjac extract) 0.008
L-proline 0.04
Water residue (total volume 50mL)

Claims (2)

  A cell activator comprising a species of Salvia hispanica, which is a Labiatae plant, or an extract thereof.   The cell activator according to claim 1, wherein the cell activator is used for external use at a concentration of 0.0001 to 20 mass%.

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