CN105168007A - Method for preparing lipidosome - Google Patents
Method for preparing lipidosome Download PDFInfo
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- CN105168007A CN105168007A CN201510596541.XA CN201510596541A CN105168007A CN 105168007 A CN105168007 A CN 105168007A CN 201510596541 A CN201510596541 A CN 201510596541A CN 105168007 A CN105168007 A CN 105168007A
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- CN
- China
- Prior art keywords
- lecithin
- surfactant
- inorganic salt
- liposome
- polyhydric alcohol
- Prior art date
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- Granted
Links
- 238000000034 method Methods 0.000 title abstract description 7
- 229940067606 lecithin Drugs 0.000 claims abstract description 39
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 claims abstract description 36
- 235000010445 lecithin Nutrition 0.000 claims abstract description 36
- 239000000787 lecithin Substances 0.000 claims abstract description 36
- 229910017053 inorganic salt Inorganic materials 0.000 claims abstract description 30
- 239000004094 surface-active agent Substances 0.000 claims abstract description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 29
- 238000003756 stirring Methods 0.000 claims abstract description 7
- 238000001816 cooling Methods 0.000 claims abstract description 6
- 239000002502 liposome Substances 0.000 claims description 43
- 150000005846 sugar alcohols Polymers 0.000 claims description 30
- 239000000203 mixture Substances 0.000 claims description 20
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 15
- 238000002360 preparation method Methods 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 11
- 208000035126 Facies Diseases 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical group [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- WTJKGGKOPKCXLL-RRHRGVEJSA-N phosphatidylcholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC WTJKGGKOPKCXLL-RRHRGVEJSA-N 0.000 claims description 9
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims description 8
- 229940083466 soybean lecithin Drugs 0.000 claims description 8
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 7
- 239000000126 substance Substances 0.000 claims description 7
- 102000002322 Egg Proteins Human genes 0.000 claims description 5
- 108010000912 Egg Proteins Proteins 0.000 claims description 5
- 241000287828 Gallus gallus Species 0.000 claims description 5
- HVUMOYIDDBPOLL-XWVZOOPGSA-N Sorbitan monostearate Chemical compound CCCCCCCCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O HVUMOYIDDBPOLL-XWVZOOPGSA-N 0.000 claims description 5
- LWZFANDGMFTDAV-BURFUSLBSA-N [(2r)-2-[(2r,3r,4s)-3,4-dihydroxyoxolan-2-yl]-2-hydroxyethyl] dodecanoate Chemical compound CCCCCCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O LWZFANDGMFTDAV-BURFUSLBSA-N 0.000 claims description 5
- 235000019437 butane-1,3-diol Nutrition 0.000 claims description 5
- 230000018044 dehydration Effects 0.000 claims description 5
- 238000006297 dehydration reaction Methods 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 210000004681 ovum Anatomy 0.000 claims description 5
- 239000000244 polyoxyethylene sorbitan monooleate Substances 0.000 claims description 5
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 5
- 229920000053 polysorbate 80 Polymers 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- 239000001509 sodium citrate Substances 0.000 claims description 5
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims description 5
- 229950006451 sorbitan laurate Drugs 0.000 claims description 5
- 235000011067 sorbitan monolaureate Nutrition 0.000 claims description 5
- 229950011392 sorbitan stearate Drugs 0.000 claims description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 4
- 229940058015 1,3-butylene glycol Drugs 0.000 claims description 2
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 claims description 2
- WERYXYBDKMZEQL-UHFFFAOYSA-N butane-1,4-diol Chemical compound OCCCCO WERYXYBDKMZEQL-UHFFFAOYSA-N 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
- 229910052938 sodium sulfate Inorganic materials 0.000 claims description 2
- 235000011152 sodium sulphate Nutrition 0.000 claims description 2
- 239000008346 aqueous phase Substances 0.000 abstract description 3
- 239000000725 suspension Substances 0.000 abstract description 3
- 239000006185 dispersion Substances 0.000 abstract description 2
- 239000004615 ingredient Substances 0.000 abstract description 2
- 239000012074 organic phase Substances 0.000 abstract 2
- 229920005862 polyol Polymers 0.000 abstract 2
- 150000003077 polyols Chemical class 0.000 abstract 2
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 description 8
- 239000012071 phase Substances 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 6
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 150000003904 phospholipids Chemical class 0.000 description 3
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 230000007096 poisonous effect Effects 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 238000001000 micrograph Methods 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000037380 skin damage Effects 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000010409 thin film Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 239000000080 wetting agent Substances 0.000 description 1
Landscapes
- Medicinal Preparation (AREA)
- Cosmetics (AREA)
Abstract
The invention provides lipidosome. The lipidosome is prepared from the following components in percentage by weight: 1-20 percent of lecithin, 1-15 percent of polyol, 0.1-2 percent of a surfactant, 0.01-0.2 percent of inorganic salt and the balance of water. The invention further provides a method for preparing the lipidosome. The method comprises the following steps: firstly, dissolving lecithin in polyol to obtain a uniform organic phase; secondly, uniformly dispersing and dissolving the surfactant and the inorganic salt in water to obtain a uniform aqueous-phase component; thirdly, slowly adding the organic phase into the aqueous phase at a uniform stirring velocity of 300-800rpm to obtain a uniform lipidosome suspension; and finally, performing ultrasonic treatment on the lipidosome suspension for 1-8 minutes with an ultrasonic power of 20-40 percent, stirring until the system is uniform, and naturally cooling the system to room temperature to obtain the lipidosome. The lipidosome prepared by the method has a stable system, can be used for simultaneously covering multiple effective ingredients, is mild to skin, and is a dispersion system with functionality and beautifying property.
Description
Technical field
The invention belongs to cosmetic technical field, relate to a kind of preparation method of liposome.
Background technology
Liposome is the hollow vesicle that phospholipid is dispersed in water formation, and the phospholipid bilayer because of its uniqueness has the structure similar with cell membrane, has affinity and be widely used in cosmetics with skin.Liposome is that cosmetic formulations provides significant performance, and it makes skin lubrication, soft, high resilience, has closure, skin can produce thin film to prevent moisture loss in skin.In addition, liposome as a kind of coated carrier, can in its phospholipid bilayer and hollow core coated fat-soluble and water-soluble active ingredient, act on deep skin, play the effect of slow release.
Closely during the last ten years, the research of liposome in Dermatology and cosmeceutical is just flourish, cosmetics containing liposome have the unrivaled advantage of Traditional makeup, and its permeability, slow-releasing, moisture retention, safety will make cosmetics to high-quality, high-grade development.
But traditional method for preparing lipidosome, owing to widely using poisonous organic reagent as methanol, chloroform etc., easily causes organic substance to remain, when being applied to the cosmetics directly spread on skin, easily damages skin.
Summary of the invention
For above-mentioned machine problem of the prior art, the invention provides a kind of preparation method of liposome, the preparation method of described this liposome solves method for preparing lipidosome of the prior art owing to widely using poisonous organic reagent, organic substance is easily caused to remain, easily to the technical problem that skin damages.
The invention provides a kind of liposome, count by weight percentage, its raw material composition and content as follows:
Lecithin 1 ~ 20.0%,
Polyhydric alcohol 1 ~ 15.0%,
Surfactant 0.1 ~ 5.0%,
Inorganic salt 0.01 ~ 2.0%,
Water surplus;
Described lecithin is one or more mixture formed in the phosphatidylcholine content soybean lecithin that is 25 ~ 70% or Ovum Gallus domesticus Flavus lecithin;
Described polyhydric alcohol is 1,2-PD, one or both mixture formed above in 1,3-PD, 1,3 butylene glycol, BDO, glycerol;
Described surfactant is polyethenoxy sorbitan laurate, one or both mixture formed in polyethenoxy sorbitan stearate, polyoxyethylene sorbitan monooleate dehydration;
Described inorganic salt is magnesium sulfate, one or more mixture formed in magnesium chloride, sodium chloride, sodium sulfate, sodium citrate.
Further, count by weight percentage, its raw material composition and content as follows:
Lecithin 1.0%,
Polyhydric alcohol 1.0%,
Surfactant 0.1%,
Inorganic salt 0.01%,
Water 97.89%;
Described lecithin is the soybean lecithin of phosphatidylcholine content 25%;
Described polyhydric alcohol is 1,2-PD;
Described surfactant is polyethenoxy sorbitan laurate;
Described inorganic salt is magnesium sulfate.
Further, count by weight percentage, its raw material composition and content as follows:
Lecithin 10.0%,
Polyhydric alcohol 7.5%,
Surfactant 2.5%,
Inorganic salt 1.0%,
Water 79.0%;
Described lecithin is the soybean lecithin of phosphatidylcholine content 50%;
Described polyhydric alcohol is 1,3 – butanediols;
Described surfactant is polyethenoxy sorbitan stearate;
Described inorganic salt is sodium chloride.
Further, count by weight percentage, its raw material composition and content as follows:
Lecithin 20.0%,
Polyhydric alcohol 15.0%,
Surfactant 5.0%,
Inorganic salt 2.0%,
Water 58%;
Described lecithin is the Ovum Gallus domesticus Flavus lecithin of phosphatidylcholine content 70%;
Described polyhydric alcohol is glycerol;
Described surfactant is polyoxyethylene sorbitan monooleate dehydration;
Described inorganic salt is sodium citrate.
Present invention also offers the preparation method of above-mentioned a kind of liposome, comprise the steps:
(1) each reacting substance is taken according to percentage by weight;
(2) lecithin is dissolved in polyhydric alcohol at 20 ~ 80 DEG C, obtains finely dispersed organic facies component;
(3) surfactant and inorganic salt disperseed and be dissolved in the water, obtaining finely dispersed water phase components;
(4) with gained water phase components in mixing speed 300 ~ 800rpm whipping step (3), the organic facies component of gained in step (2) is slowly at the uniform velocity added wherein simultaneously, after stirring, namely obtain liposome turbid liquor;
(5) with the liposome turbid liquor 1 ~ 8min of ultrasonic power 20% ~ 40% ultrasonic step (4) gained, be stirred to system all in the lump natural cooling be down to room temperature, obtain liposome.
The preparation method of a kind of liposome of the present invention, is first dissolved in polyhydric alcohol by lecithin, joins in aqueous phase under high velocity agitation, forms the suspension of liposome.Then, the liposome turbid liquor obtained is carried out ultrasonic under ultrasonic probe, form the liposome that particle diameter is little, solve the problem of the organic solvent residual that above-mentioned traditional liposomal easily causes.
Present invention employs there is moisture-keeping function polyhydric alcohol as dissolution with solvents lecithin, and have employed suitable surfactant and inorganic salt compound scheme, prepared safety non-toxic and the little and uniform liposomal systems of particle diameter.Simultaneously because polyhydric alcohol belongs to again wetting agent conventional in cosmetics, serve multiple technique effect.
The present invention compares with prior art, and its technological progress is significant.Preparation technology of the present invention is simple, energy consumption is low, and the liposomal systems of formation is stablized, can coated several effective ingredient simultaneously, and gentle to skin, is the functional and esthetics dispersion had concurrently.
Accompanying drawing explanation
Fig. 1 is the electron scanning micrograph figure of the liposome of embodiment 1 gained.
Detailed description of the invention
Also by reference to the accompanying drawings the present invention is set forth further below by specific embodiment, but do not limit the present invention.
Microscope used in the embodiment of the present invention is that Holland manufactures FEIQuanta200FEG type scanning electron microscope.
embodiment 1
A kind of liposome, counts by weight percentage, each raw material composition and content as follows:
Lecithin 1.0%
Polyhydric alcohol 1.0%
Surfactant 0.1%
Inorganic salt 0.01%
Water 97.89%
Described lecithin is PC(phosphatidylcholine) soybean lecithin of content 25%, the technical grade product that Beijing Merya's Lecithin Co., Ltd. produces;
Described polyhydric alcohol is 1,2-PD, the AG product that Chemical Reagent Co., Ltd., Sinopharm Group produces;
Described surfactant is polyethenoxy sorbitan laurate, the chemical grade product that Chemical Reagent Co., Ltd., Sinopharm Group produces;
Described inorganic salt is magnesium sulfate, the AG product that Chemical Reagent Co., Ltd., Sinopharm Group produces.
The preparation method of above-mentioned a kind of liposome, specifically comprises the steps:
(1) lecithin is dissolved in polyhydric alcohol at 20 ~ 80 DEG C, obtains finely dispersed organic facies component;
(2) surfactant and inorganic salt disperseed and be dissolved in the water, obtaining finely dispersed water phase components;
(3) with gained water phase components in mixing speed 300 ~ 800rpm whipping step (2), the organic facies component of gained in step (1) is slowly at the uniform velocity added wherein simultaneously, after stirring, namely obtain liposome turbid liquor;
(4) with the liposome turbid liquor 1 ~ 8min of ultrasonic power 20% ~ 40% ultrasonic step (3) gained, be stirred to system all in the lump natural cooling be down to room temperature, obtain liposome.
As shown in Figure 1, as can be seen from Figure 1 emulsion particle distribution is homogeneous, and size is at about 120nm for the scanning electron microscope (SEM) photograph of the liposome of above-mentioned gained.
embodiment 2
A kind of liposome, counts by weight percentage, each raw material composition and content as follows:
Lecithin 10.0%
Polyhydric alcohol 7.5%
Surfactant 2.5%
Inorganic salt 1.0%
Water 79.0%
Described lecithin is the soybean lecithin of PC content 50%, the technical grade product that Beijing Merya's Lecithin Co., Ltd. produces;
Described polyhydric alcohol is 1,3 – butanediols, the AG product that Chemical Reagent Co., Ltd., Sinopharm Group produces;
Described surfactant is polyethenoxy sorbitan stearate, the chemical grade product that Chemical Reagent Co., Ltd., Sinopharm Group produces;
Described inorganic salt is sodium chloride, the AG product that Chemical Reagent Co., Ltd., Sinopharm Group produces.
The preparation method of above-mentioned a kind of liposome, specifically comprises the steps:
(1) lecithin is dissolved in polyhydric alcohol at 20 ~ 80 DEG C, obtains finely dispersed organic facies component;
(2) surfactant and inorganic salt disperseed and be dissolved in the water, obtaining finely dispersed water phase components;
(3) with gained water phase components in mixing speed 300 ~ 800rpm whipping step (2), the organic facies component of gained in step (1) is slowly at the uniform velocity added wherein simultaneously, after stirring, namely obtain liposome turbid liquor;
(4) with the liposome turbid liquor 1 ~ 8min of ultrasonic power 20% ~ 40% ultrasonic step (3) gained, be stirred to system all in the lump natural cooling be down to room temperature, obtain liposome.
embodiment 3
A kind of liposome, counts by weight percentage, each raw material composition and content as follows:
Lecithin 20.0%
Polyhydric alcohol 15.0%
Surfactant 5.0%
Inorganic salt 2.0%
Water 58%
Described lecithin is the Ovum Gallus domesticus Flavus lecithin of PC content 70%, the technical grade product that Beijing Merya's Lecithin Co., Ltd. produces;
Described polyhydric alcohol is glycerol, the AG product that Chemical Reagent Co., Ltd., Sinopharm Group produces;
Described surfactant is polyoxyethylene sorbitan monooleate dehydration, the chemical grade product that Chemical Reagent Co., Ltd., Sinopharm Group produces;
Described inorganic salt is sodium citrate, the AG product that Chemical Reagent Co., Ltd., Sinopharm Group produces.
The preparation method of above-mentioned a kind of liposome, specifically comprises the steps:
(1) lecithin is dissolved in polyhydric alcohol at 20 ~ 80 DEG C, obtains finely dispersed organic facies component;
(2) surfactant and inorganic salt disperseed and be dissolved in the water, obtaining finely dispersed water phase components;
(3) with gained water phase components in mixing speed 300 ~ 800rpm whipping step (2), the organic facies component of gained in step (1) is slowly at the uniform velocity added wherein simultaneously, after stirring, namely obtain liposome turbid liquor;
(4) with the liposome turbid liquor 1 ~ 8min of ultrasonic power 20% ~ 40% ultrasonic step (3) gained, be stirred to system all in the lump natural cooling be down to room temperature, obtain liposome.
Foregoing be only the present invention conceive under basic explanation, and according to any equivalent transformation that technical scheme of the present invention is done, all should protection scope of the present invention be belonged to.
Claims (5)
1. a liposome, is characterized in that counting by weight percentage, its raw material composition and content as follows:
Lecithin 1 ~ 20.0%,
Polyhydric alcohol 1 ~ 15.0%,
Surfactant 0.1 ~ 5.0%,
Inorganic salt 0.01 ~ 2.0%,
Water surplus;
Described lecithin is one or more mixture formed in the phosphatidylcholine content soybean lecithin that is 25 ~ 70% or Ovum Gallus domesticus Flavus lecithin;
Described polyhydric alcohol is 1,2-PD, one or both mixture formed above in 1,3-PD, 1,3 butylene glycol, BDO, glycerol;
Described surfactant is polyethenoxy sorbitan laurate, one or both mixture formed in polyethenoxy sorbitan stearate, polyoxyethylene sorbitan monooleate dehydration;
Described inorganic salt is magnesium sulfate, one or more mixture formed in magnesium chloride, sodium chloride, sodium sulfate, sodium citrate.
2. a kind of liposome as claimed in claim 1, is characterized in that counting by weight percentage, its raw material composition and content as follows:
Lecithin 1.0%,
Polyhydric alcohol 1.0%,
Surfactant 0.1%,
Inorganic salt 0.01%,
Water 97.89%;
Described lecithin is the soybean lecithin of phosphatidylcholine content 25%;
Described polyhydric alcohol is 1,2-PD;
Described surfactant is polyethenoxy sorbitan laurate;
Described inorganic salt is magnesium sulfate.
3. a kind of liposome as claimed in claim 1, is characterized in that counting by weight percentage, its raw material composition and content as follows:
Lecithin 10.0%,
Polyhydric alcohol 7.5%,
Surfactant 2.5%,
Inorganic salt 1.0%,
Water 79.0%;
Described lecithin is the soybean lecithin of phosphatidylcholine content 50%;
Described polyhydric alcohol is 1,3 – butanediols;
Described surfactant is polyethenoxy sorbitan stearate;
Described inorganic salt is sodium chloride.
4. a kind of liposome as claimed in claim 1, is characterized in that counting by weight percentage, its raw material composition and content as follows:
Lecithin 20.0%,
Polyhydric alcohol 15.0%,
Surfactant 5.0%,
Inorganic salt 2.0%,
Water 58%;
Described lecithin is the Ovum Gallus domesticus Flavus lecithin of phosphatidylcholine content 70%;
Described polyhydric alcohol is glycerol;
Described surfactant is polyoxyethylene sorbitan monooleate dehydration;
Described inorganic salt is sodium citrate.
5. the preparation method of a kind of liposome described in claims 1,2,3 or 4, is characterized in that comprising the steps:
(1) each reacting substance is taken according to percentage by weight;
(2) lecithin is dissolved in polyhydric alcohol at 20 ~ 80 DEG C, obtains finely dispersed organic facies component;
(3) surfactant and inorganic salt disperseed and be dissolved in the water, obtaining finely dispersed water phase components;
(4) with gained water phase components in mixing speed 300 ~ 800rpm whipping step (3), the organic facies component of gained in step (2) is slowly at the uniform velocity added wherein simultaneously, after stirring, namely obtain liposome turbid liquor;
(5) with the liposome turbid liquor 1 ~ 8min of ultrasonic power 20% ~ 40% ultrasonic step (4) gained, be stirred to system all in the lump natural cooling be down to room temperature, obtain liposome.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510596541.XA CN105168007B (en) | 2015-09-18 | 2015-09-18 | A kind of preparation method of liposome |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510596541.XA CN105168007B (en) | 2015-09-18 | 2015-09-18 | A kind of preparation method of liposome |
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| CN105168007A true CN105168007A (en) | 2015-12-23 |
| CN105168007B CN105168007B (en) | 2018-10-12 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106214506A (en) * | 2016-08-29 | 2016-12-14 | 珀莱雅化妆品股份有限公司 | A kind of preparation method of the multilamellar lipid vesicle wrapping up Forskolin |
| CN107496245A (en) * | 2017-09-15 | 2017-12-22 | 澳宝化妆品（惠州）有限公司 | A kind of anti-aging liposome composition and its preparation method and application |
| CN113559007A (en) * | 2021-08-11 | 2021-10-29 | 浙江宜格企业管理集团有限公司 | Preparation method of liposome with whitening and anti-aging effects |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010124540A1 (en) * | 2009-04-29 | 2010-11-04 | 上海家化联合股份有限公司 | Flexible liposome of resveratrol and preparation method thereof |
-
2015
- 2015-09-18 CN CN201510596541.XA patent/CN105168007B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010124540A1 (en) * | 2009-04-29 | 2010-11-04 | 上海家化联合股份有限公司 | Flexible liposome of resveratrol and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 章贤明: "S0D脂质体的制备研究", 《生物医学工程学杂志》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106214506A (en) * | 2016-08-29 | 2016-12-14 | 珀莱雅化妆品股份有限公司 | A kind of preparation method of the multilamellar lipid vesicle wrapping up Forskolin |
| CN106214506B (en) * | 2016-08-29 | 2018-12-07 | 珀莱雅化妆品股份有限公司 | A kind of preparation method for the multilayer lipid vesicle wrapping up Forskolin |
| CN107496245A (en) * | 2017-09-15 | 2017-12-22 | 澳宝化妆品（惠州）有限公司 | A kind of anti-aging liposome composition and its preparation method and application |
| CN107496245B (en) * | 2017-09-15 | 2020-04-03 | 澳宝化妆品（惠州）有限公司 | Anti-aging liposome composition and preparation method and application thereof |
| CN113559007A (en) * | 2021-08-11 | 2021-10-29 | 浙江宜格企业管理集团有限公司 | Preparation method of liposome with whitening and anti-aging effects |
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| Publication number | Publication date |
|---|---|
| CN105168007B (en) | 2018-10-12 |
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