CN106726641A - A kind of many vesicles of cladding D panthenols and preparation method thereof - Google Patents
A kind of many vesicles of cladding D panthenols and preparation method thereof Download PDFInfo
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- CN106726641A CN106726641A CN201710050184.6A CN201710050184A CN106726641A CN 106726641 A CN106726641 A CN 106726641A CN 201710050184 A CN201710050184 A CN 201710050184A CN 106726641 A CN106726641 A CN 106726641A
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- organic solvent
- osmotic pressure
- pressure regulator
- amino acid
- pantothenyl aleohol
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/42—Amides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/41—Particular ingredients further characterized by their size
- A61K2800/412—Microsized, i.e. having sizes between 0.1 and 100 microns
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Abstract
A kind of many vesicles of cladding D panthenols, are made up of phosphatide, neutral lipid, cholesterol, organic solvent, hydrophilic emulsifier, D panthenols, amino acid, osmotic pressure regulator and deionized water.Be dissolved in organic solvent for phosphatide, cholesterol and neutral lipid by the present invention, used as organic phase；D panthenols, amino acid, osmotic pressure regulator and deionized water are mixed as interior water phase；The organic phase component of homogeneous, interior water phase components are added, and w/o type colostric fluid is obtained；Hydrophilic emulsifier, amino acid, osmotic pressure regulator and deionized water are mixed, is disperseed as outer water phase；The outer water phase components of homogeneous, add w/o type colostric fluid component, and ultrasound is carried out after homogeneous, are stirred for being cooled to room temperature, form W/O/W type multiple emulsions；Multiple emulsion is scattered in Freamine Ⅲ again, is passed through the organic solvent during nitrogen removes multiple emulsion, obtain final product many vesicles suspension of cladding D panthenols.The present invention improves the stability of D panthenols.
Description
Technical field
The invention belongs to cosmetic field, it is related to a kind of liposome, specifically a kind of many vesicas for coating D-pantothenyl aleohol
Liposome and preparation method thereof.
Background technology
D-pantothenyl aleohol is the premise of vitamin B5, therefore is the water-soluble active thing of stabilization also known as dexpanthenol, is widely used in
In cosmetics, medicine, food service industry.Its application performance in cosmetics shows as：Nursing role on skin shows as depth
Enter the NMF of infiltration, stimulate the growth of epithelial cell, promote wound healing, play antiinflammation；Nursing role table on hair
It is now lasting moisture-keeping functions, prevents hair jag, be damaged, increase the density of hair, improves the gloss of hair quality；The nursing of nail
On show as improve nail hydrability, assign nail pliability.But D-pantothenyl aleohol is sensitive to heat, acid, alkali, long-time high-temperature heating
Racemic can be caused, physiologically active declines, and can be hydrolyzed under the conditions of strong acid and strong base, and stability is poor, it is limited in cosmetics
Further application, therefore exploitation has, and functionality is strong, the D-pantothenyl aleohol liposome class cosmetics of good stability are by with wide
Prospect and application value.
Multivesicular liposome (multivesieular liposomes, MVLs), is a kind of structure also known as many vesicles
Special novel lipid body preparation, its discontinuous aqueous inner chamber is separated by nonconcentric(al) lipoid and immobilized artificial membrane, and formation is permitted
More trickle vesica.MVLS particles are in honeycomb, and particle size is typically between 1-100 μm.The structure of many vesicas of MVLs makes it
Volume is encapsulated with more water-soluble substanceses, hydroecium volume accounts for the 95% of particle overall volume, the load active matter of its water-soluble substances
Amount is more much higher than traditional unilamellar liposome and multilamellar liposome；When certain capsules rupture, active material from rupture vesica
Disengage, complete vesica still can maintain the original state, thus have good slowly releasing effect；The non-concentric structure of lipid layer makes lipid
The stability of body increases, and the release time of active matter extends.
Cladding for hydroaropic substance is needed by means of many vesicles, and D-pantothenyl aleohol is coated on into interior water phase, is formed
A kind of coated carrier structure for being directed to D-pantothenyl aleohol.To improve the stability of D-pantothenyl aleohol, and then improve the activity of D-pantothenyl aleohol.
At present, the research on many vesicles carrier techniques has had a lot, and mostly coating medicine is applied to medical treatment
Industry, its preparation technology is more complicated, but many vesicles of cladding D-pantothenyl aleohol and be applied in cosmetics there is not yet
It is open.
The content of the invention
For above-mentioned technical problem of the prior art, the invention provides a kind of many vesicles for coating D-pantothenyl aleohol
And preparation method thereof, described many vesicles of this cladding D-pantothenyl aleohol and preparation method thereof will solve of the prior art
D-pantothenyl aleohol is sensitive to heat, acid, alkali, and long-time high-temperature heating can cause racemic, and physiologically active declines, under the conditions of strong acid and strong base
Can hydrolyze, the technical problem of stability difference.
The invention provides a kind of many vesicles for coating D-pantothenyl aleohol, count by weight percentage, its raw material composition
And content is as follows：
Phosphatidase 1 .0 ~ 20.0%；
Neutral lipid 1.0 ~ 10.0%；
Cholesterol 1.0 ~ 10.0%；
Organic solvent 5.0 ~ 20.0%；
D-pantothenyl aleohol 0.01 ~ 5.0%；
Hydrophilic emulsifier 0.1 ~ 12.0%；
Amino acid 0.1 ~ 1.0%；
Osmotic pressure regulator 2.0 ~ 8.0%；
Deionized water surplus；
Described phosphoric acid is lecithin, hydrolecithin, soybean lecithin, phosphatidyl-ethanolamine, DSPC, two
In dimyristoylphosphatidycholine, DOPE or DPPG one or two with
On the mixture that is constituted；
Described neutral lipid is olein, glyceric acid, glyceryl laurate ester, decanoin, three sad glycerine
One or more mixtures for being constituted in ester or vitamin E；
Described organic solvent is by one or more mixing for constituting in ether, ethanol, butanol or ethyl acetate
Thing；
Described hydrophilic emulsifier is hydrophilic Tween 80, polyox-yethylene-polyoxypropylene block copolymer, peptides bimolecular oleophylic
Table work, PEG-100 stearates, stearine, arachidic alcohol and docosyl alcohol and arachidic alcohol glucoside, cetostearyl alcohol and whale
One kind or two in wax stearyl glucoside, AEO, Compritol 888 ATO or cetearyl glucoside
The mixture constituted more than kind；
Described amino acid is lysine, asparatate, serine, L-arginine, alanine, L-aminobutanedioic acid, glutamic acid, sweet
One or more mixture in propylhomoserin, histidine, tryptophan, tyrosine, proline, leucine or valine；
Described osmotic pressure regulator is one or more the mixture in glucose, sucrose or physiological saline.
Further, many vesicles of above-mentioned a kind of cladding D-pantothenyl aleohol, count by weight percentage, its raw material group
Into and content it is as follows：
Phosphatidase 1 .0%；
Neutral lipid 1.0%；
Cholesterol 1.0%；
Organic solvent 5.0%；
D-pantothenyl aleohol 0.01%；
Hydrophilic emulsifier 0.1%；
Amino acid 0.1%；
Osmotic pressure regulator 2.0%；
Deionized water surplus；
Described phosphatide is lecithin；
Described neutral grease is olein；
Described organic solvent is ether；
Described hydrophilic emulsifier is Tween 80；
Described amino acid is lysine；
Described osmotic pressure regulator is glucose.
Further, many vesicles of above-mentioned a kind of cladding D-pantothenyl aleohol, count by weight percentage, its raw material group
Into and content it is as follows：
Phosphatidase 1 0.0%；
Neutral lipid 5.0%；
Cholesterol 5.0%；
Organic solvent 12.5%；
D-pantothenyl aleohol 2.5%；
Hydrophilic emulsifier 6.0%；
Amino acid 0.5%；
Osmotic pressure regulator 5.0%；
Deionized water surplus；
Described phosphatide is the mixture of one or two compositions in hydrolecithin, soybean lecithin；
Described neutral substance is glyceric acid；
Described organic solvent is ethanol；
Described hydrophilic emulsifier be polyox-yethylene-polyoxypropylene block copolymer, PEG-100 stearates in one kind or
Two kinds of mixtures for being constituted；
Described amino acid is asparatate；
Described osmotic pressure regulator is sucrose.
Further, many vesicles of above-mentioned a kind of cladding D-pantothenyl aleohol, count by weight percentage, its raw material group
Into and content it is as follows：
Phosphatidase 2 0.0%；
Neutral lipid 10.0%；
Cholesterol 10.0%；
Organic solvent 20.0%；
D-pantothenyl aleohol 5.0%；
Hydrophilic emulsifier 12.0%；
Amino acid/11 .0%；
Osmotic pressure regulator 8.0%；
Deionized water surplus；
Described phosphoric acid is phosphatidyl-ethanolamine；
Described neutral lipid is by one or two mixtures for constituting in tricaprylin, vitamin E；
Described organic solvent is butanol；
Described hydrophilic emulsifier is in the hydrophilic table work of peptides bimolecular oleophylic, cetostearyl alcohol and cetearyl glucoside
One or two mixtures for being constituted；
Described amino acid is L-arginine；
Described osmotic pressure regulator is physiological saline.
Present invention also offers a kind of preparation method of many vesicles of above-mentioned cladding D-pantothenyl aleohol, including following step
Suddenly：
1) phosphatide, neutral lipid, cholesterol, organic solvent, hydrophilic emulsifier, D-pantothenyl aleohol, ammonia are weighed according to mass percent
Base acid, osmotic pressure regulator and deionized water；
2) phosphatide, cholesterol and neutral lipid are dissolved in organic solvent, as organic phase；
3) Freamine Ⅲ of 1 ~ 100mmol/L is configured, by D-pantothenyl aleohol, 30 ~ 60% Freamine Ⅲ, 30 ~ 60% osmotic pressure
Conditioning agent and the mixing of 30 ~ 60% deionized water, as interior water phase；
4) by step 3）Water is added in organic phase in middle gained, with homogeneous speed 7200~30000rpm homogeneous organic phase groups
Point, while interior water phase components are added thereto, 1~15min of homogeneous, w/o type colostric fluid is obtained；
5) by hydrophilic emulsifier, the Freamine Ⅲ of remaining content, the osmotic pressure regulator of remaining content and remaining content
The mixed solution of deionized water, as outer water phase；
6) with the outer water phase components of homogeneous 7200~30000rpm of speed homogeneous, while by step 4）Obtained w/o type colostric fluid group
Point be added thereto, after 1~15min of homogeneous, select ultrasonic power 20%~30%, ultrasound stops 5 seconds in 5 seconds, to emulsion ultrasound 1~
20min, to system, natural cooling is down to room temperature in the lump for stirring, is made W/O/W type multiple emulsions；
7) configuration and step 3) in concentration identical Freamine Ⅲ, the amino acid that the multiple emulsion that will be obtained is scattered in configuration is molten
In liquid, wherein multiple emulsion and the volume ratio of Freamine Ⅲ is 5:1~1:5, be passed through nitrogen remove multiple emulsion in it is organic
Solvent, is obtained many vesicles suspension of cladding D-pantothenyl aleohol.
The step 7) in Freamine Ⅲ effect be influence the aqueous solution pH, adjust oil-water interfaces phosphatide surface electricity
Lotus, improves the electrostatic stability of many vesicles, reduces many vesicles and blows down breaking during removing organic solvent in nitrogen
Newborn phenomenon, and the forming process of many vesicles is not involved in, therefore the content of amino acid does not include this part in formula.
Be dissolved in organic solvent for phosphatide, cholesterol and neutral lipid by the present invention, used as organic phase；By D-pantothenyl aleohol, amino
Acid, osmotic pressure regulator and deionized water are well mixed as interior water phase；The organic phase component of homogeneous, interior water phase components are added,
W/o type colostric fluid is obtained；Hydrophilic emulsifier, amino acid, osmotic pressure regulator and deionized water are mixed, be uniformly dispersed conduct
Outer water phase；The outer water phase components of homogeneous, the w/o type colostric fluid component prepared before addition carries out ultrasound, is stirred for cooling after homogeneous
To room temperature, W/O/W type multiple emulsions are formed；Multiple emulsion is scattered in Freamine Ⅲ again, is passed through nitrogen and is removed multiple breast
Organic solvent in liquid, obtains final product many vesicles suspension of cladding D-pantothenyl aleohol.
Present invention employs suitable phosphatide, neutral lipid, cholesterol, organic solvent, amino acid, osmotic pressure regulator and
The compound scheme of deionized water.Two step emulsion processes are used in preparation process of the invention, and carries out ultrasound, therefore, final institute
The particle diameter of the dispersed phase particles of many vesicles for obtaining is more uniform, and its preparation process is simple, energy consumption are low, the emulsion of formation
Stable system, not only provides suitable carrier to D-pantothenyl aleohol, improves the stability of D-pantothenyl aleohol, and it is gentle to skin, inhales
Receive fast, have feature and esthetics concurrently.
The present invention is compared with prior art, and its technological progress is significant.The present invention is prepared for a kind of cladding D-pantothenyl aleohol
Many vesicles, for the cladding of D-pantothenyl aleohol provides suitable carrier, while improve the stability of D-pantothenyl aleohol, and then improve D-
The activity of panthenol, develops with functionality is strong, the good stability cosmetics containing D-pantothenyl aleohol active matter.
Brief description of the drawings
Fig. 1 is the microphotograph of many vesicles of the cladding D-pantothenyl aleohol of the gained of embodiment 1.
Specific embodiment
The present invention is expanded on further below by specific embodiment and with reference to accompanying drawing, but is not intended to limit the present invention.
Embodiment 1
A kind of many vesicles for coating D-pantothenyl aleohol, count by weight percentage, its raw material composition and content are as follows：
Phosphatidase 1 .0%；
Neutral lipid 1.0%；
Cholesterol 1.0%；
Organic solvent 5.0%；
D-pantothenyl aleohol 0.01%；
Hydrophilic emulsifier 0.1%；
Amino acid 0.1%；
Osmotic pressure regulator 2.0%；
Deionized water surplus；
Described phosphatide is lecithin；
Described neutral grease is olein；
Described organic solvent is ether；
Described hydrophilic emulsifier is Tween 80；
Described amino acid is lysine；
Described osmotic pressure regulator is glucose；
A kind of preparation method of many vesicles of above-mentioned cladding D-pantothenyl aleohol, specifically includes following steps：
1) phosphatide, neutral lipid, cholesterol, organic solvent, hydrophilic emulsifier, D-pantothenyl aleohol, ammonia are weighed according to mass percent
Base acid, osmotic pressure regulator and deionized water；
2) phosphatide, cholesterol and neutral lipid are dissolved in organic solvent, as organic phase；
3) Freamine Ⅲ of 1 ~ 100mmol/L is configured, by D-pantothenyl aleohol, 30 ~ 60% Freamine Ⅲ, 30 ~ 60% osmotic pressure
Conditioning agent and the mixing of 30 ~ 60% deionized water, as interior water phase；
4) by step 3）Water is added in organic phase in middle gained, with homogeneous speed 7200~30000rpm homogeneous organic phase groups
Point, while interior water phase components are added thereto, 1~15min of homogeneous, w/o type colostric fluid is obtained；
5) by hydrophilic emulsifier, the Freamine Ⅲ of remaining content, the osmotic pressure regulator of remaining content and remaining content
The mixed solution of deionized water, as outer water phase；
6) with the outer water phase components of homogeneous 7200~30000rpm of speed homogeneous, while by step 4）Obtained w/o type colostric fluid group
Point be added thereto, after 1~15min of homogeneous, select ultrasonic power 20%~30%, ultrasound stops 5 seconds in 5 seconds, to emulsion ultrasound 1~
20min, to system, natural cooling is down to room temperature in the lump for stirring, is made W/O/W type multiple emulsions；
7) configuration and step 3) in concentration identical Freamine Ⅲ, the amino acid that the multiple emulsion that will be obtained is scattered in configuration is molten
In liquid, wherein multiple emulsion and the volume ratio of Freamine Ⅲ is 5:1~1:5, be passed through nitrogen remove multiple emulsion in it is organic
Solvent, is obtained many vesicles suspension of cladding D-pantothenyl aleohol.
Embodiment 2
A kind of many vesicles for coating D-pantothenyl aleohol, count by weight percentage, its raw material composition and content are as follows：
Phosphatidase 1 0.0%；
Neutral lipid 5.0%；
Cholesterol 5.0%；
Organic solvent 12.5%；
D-pantothenyl aleohol 2.5%；
Hydrophilic emulsifier 6.0%；
Amino acid 0.5%；
Osmotic pressure regulator 5.0%；
Deionized water surplus；
Described phosphatide is the mixture of one or two compositions in hydrolecithin, soybean lecithin；
Described neutral substance is glyceric acid；
Described organic solvent is ethanol；
Described hydrophilic emulsifier be polyox-yethylene-polyoxypropylene block copolymer, PEG-100 stearates in one kind or
Two kinds of mixtures for being constituted；
Described amino acid is asparatate；
Described osmotic pressure regulator is sucrose；
A kind of preparation method of many vesicles of above-mentioned cladding D-pantothenyl aleohol, specifically includes following steps：
1) phosphatide, neutral lipid, cholesterol, organic solvent, hydrophilic emulsifier, D-pantothenyl aleohol, ammonia are weighed according to mass percent
Base acid, osmotic pressure regulator and deionized water；
2) phosphatide, cholesterol and neutral lipid are dissolved in organic solvent, as organic phase；
3) Freamine Ⅲ of 1 ~ 100mmol/L is configured, by D-pantothenyl aleohol, 30 ~ 60% Freamine Ⅲ, 30 ~ 60% osmotic pressure
Conditioning agent and the mixing of 30 ~ 60% deionized water, as interior water phase；
4) by step 3）Water is added in organic phase in middle gained, with homogeneous speed 7200~30000rpm homogeneous organic phase groups
Point, while interior water phase components are added thereto, 1~15min of homogeneous, w/o type colostric fluid is obtained；
5) by hydrophilic emulsifier, the Freamine Ⅲ of remaining content, the osmotic pressure regulator of remaining content and remaining content
The mixed solution of deionized water, as outer water phase；
6) with the outer water phase components of homogeneous 7200~30000rpm of speed homogeneous, while by step 4）Obtained w/o type colostric fluid group
Point be added thereto, after 1~15min of homogeneous, select ultrasonic power 20%~30%, ultrasound stops 5 seconds in 5 seconds, to emulsion ultrasound 1~
20min, to system, natural cooling is down to room temperature in the lump for stirring, is made W/O/W type multiple emulsions；
7) configuration and step 3) in concentration identical Freamine Ⅲ, the amino acid that the multiple emulsion that will be obtained is scattered in configuration is molten
In liquid, wherein multiple emulsion and the volume ratio of Freamine Ⅲ is 5:1~1:5, be passed through nitrogen remove multiple emulsion in it is organic
Solvent, is obtained many vesicles suspension of cladding D-pantothenyl aleohol.
Embodiment 3
A kind of many vesicles for coating D-pantothenyl aleohol, count by weight percentage, its raw material composition and content are as follows：
Phosphatidase 2 0.0%；
Neutral lipid 10.0%；
Cholesterol 10.0%；
Organic solvent 20.0%；
D-pantothenyl aleohol 5.0%；
Hydrophilic emulsifier 12.0%；
Amino acid/11 .0%；
Osmotic pressure regulator 8.0%；
Deionized water surplus；
Described phosphoric acid is phosphatidyl-ethanolamine；
Described neutral lipid is by one or two mixtures for constituting in tricaprylin, vitamin E；
Described organic solvent is butanol；
Described hydrophilic emulsifier is in the hydrophilic table work of peptides bimolecular oleophylic, cetostearyl alcohol and cetearyl glucoside
One or two composition mixtures；
Described amino acid is L-arginine；
Described osmotic pressure regulator is physiological saline；
A kind of preparation method of many vesicles of above-mentioned cladding D-pantothenyl aleohol, specifically includes following steps：
1) phosphatide, neutral lipid, cholesterol, organic solvent, hydrophilic emulsifier, D-pantothenyl aleohol, ammonia are weighed according to mass percent
Base acid, osmotic pressure regulator and deionized water；
2) phosphatide, cholesterol and neutral lipid are dissolved in organic solvent, as organic phase；
3) Freamine Ⅲ of 1 ~ 100mmol/L is configured, by D-pantothenyl aleohol, 30 ~ 60% Freamine Ⅲ, 30 ~ 60% osmotic pressure
Conditioning agent and the mixing of 30 ~ 60% deionized water, as interior water phase；
4) by step 3）Water is added in organic phase in middle gained, with homogeneous speed 7200~30000rpm homogeneous organic phase groups
Point, while interior water phase components are added thereto, 1~15min of homogeneous, w/o type colostric fluid is obtained；
5) by hydrophilic emulsifier, the Freamine Ⅲ of remaining content, the osmotic pressure regulator of remaining content and remaining content
The mixed solution of deionized water, as outer water phase；
6) with the outer water phase components of homogeneous 7200~30000rpm of speed homogeneous, while by step 4）Obtained w/o type colostric fluid group
Point be added thereto, after 1~15min of homogeneous, select ultrasonic power 20%~30%, ultrasound stops 5 seconds in 5 seconds, to emulsion ultrasound 1~
20min, to system, natural cooling is down to room temperature in the lump for stirring, is made W/O/W type multiple emulsions；
7) configuration and step 3) in concentration identical amino acid acid solution, the multiple emulsion that will be obtained is scattered in the amino acid of configuration
In solution, wherein multiple emulsion and the volume ratio of Freamine Ⅲ is 5:1~1:5, be passed through nitrogen remove multiple emulsion in have
Machine solvent, is obtained many vesicles suspension of cladding D-pantothenyl aleohol.
The above is only the basic explanation under present inventive concept, and according to technical scheme made it is any etc.
Effect conversion, all should belong to protection scope of the present invention.
Claims (5)
1. it is a kind of coat D-pantothenyl aleohol many vesicles, it is characterised in that count by weight percentage, its raw material constitute and contain
Amount is as follows：
Phosphatidase 1 .0 ~ 20.0%；
Neutral lipid 1.0 ~ 10.0%；
Cholesterol 1.0 ~ 10.0%；
Organic solvent 5.0 ~ 20.0%；
D-pantothenyl aleohol 0.01 ~ 5.0%；
Hydrophilic emulsifier 0.1 ~ 12.0%；
Amino acid 0.1 ~ 1.0%；
Osmotic pressure regulator 2.0 ~ 8.0%；
Deionized water surplus；
Described phosphoric acid is lecithin, hydrolecithin, soybean lecithin, phosphatidyl-ethanolamine, DSPC, two
In dimyristoylphosphatidycholine, DOPE or DPPG one or two with
On the mixture that is constituted；
Described neutral lipid is olein, glyceric acid, glyceryl laurate ester, decanoin, three sad glycerine
One or more mixtures for being constituted in ester or vitamin E；
Described organic solvent is by one or more mixing for constituting in ether, ethanol, butanol or ethyl acetate
Thing；
Described hydrophilic emulsifier is hydrophilic Tween 80, polyox-yethylene-polyoxypropylene block copolymer, peptides bimolecular oleophylic
Table work, PEG-100 stearates, stearine, arachidic alcohol and docosyl alcohol and arachidic alcohol glucoside, cetostearyl alcohol and whale
One kind or two in wax stearyl glucoside, AEO, Compritol 888 ATO or cetearyl glucoside
The mixture constituted more than kind；
Described amino acid is lysine, asparatate, serine, L-arginine, alanine, L-aminobutanedioic acid, glutamic acid, sweet
One or more mixture in propylhomoserin, histidine, tryptophan, tyrosine, proline, leucine or valine；
Described osmotic pressure regulator is one or more the mixture in glucose, sucrose or physiological saline.
2. a kind of many vesicles for coating D-pantothenyl aleohol as claimed in claim 1, it is characterised in that by weight percentage
Calculate, its raw material composition and content are as follows：
Phosphatidase 1 .0%；
Neutral lipid 1.0%；
Cholesterol 1.0%；
Organic solvent 5.0%；
D-pantothenyl aleohol 0.01%；
Hydrophilic emulsifier 0.1%；
Amino acid 0.1%；
Osmotic pressure regulator 2.0%；
Deionized water surplus；
Described phosphatide is lecithin；
Described neutral grease is olein；
Described organic solvent is ether；
Described hydrophilic emulsifier is Tween 80；
Described amino acid is lysine；
Described osmotic pressure regulator is glucose.
3. a kind of many vesicles for coating D-pantothenyl aleohol as claimed in claim 1, it is characterised in that by weight percentage
Calculate, its raw material composition and content are as follows：
Phosphatidase 1 0.0%；
Neutral lipid 5.0%；
Cholesterol 5.0%；
Organic solvent 12.5%；
D-pantothenyl aleohol 2.5%；
Hydrophilic emulsifier 6.0%；
Amino acid 0.5%；
Osmotic pressure regulator 5.0%；
Deionized water surplus；
Described phosphatide is the mixture of one or two compositions in hydrolecithin, soybean lecithin；
Described neutral substance is glyceric acid；
Described organic solvent is ethanol；
Described hydrophilic emulsifier be polyox-yethylene-polyoxypropylene block copolymer, PEG-100 stearates in one kind or
Two kinds of mixtures for being constituted；
Described amino acid is asparatate；
Described osmotic pressure regulator is sucrose.
4. a kind of many vesicles for coating D-pantothenyl aleohol as claimed in claim 1, it is characterised in that by weight percentage
Calculate, its raw material composition and content are as follows：
Phosphatidase 2 0.0%；
Neutral lipid 10.0%；
Cholesterol 10.0%；
Organic solvent 20.0%；
D-pantothenyl aleohol 5.0%；
Hydrophilic emulsifier 12.0%；
Amino acid/11 .0%；
Osmotic pressure regulator 8.0%；
Deionized water surplus；
Described phosphoric acid is phosphatidyl-ethanolamine；
Described neutral lipid is by one or two mixtures for constituting in tricaprylin, vitamin E；
Described organic solvent is butanol；
Described hydrophilic emulsifier is in the hydrophilic table work of peptides bimolecular oleophylic, cetostearyl alcohol and cetearyl glucoside
One or two mixtures for being constituted；
Described amino acid is L-arginine；
Described osmotic pressure regulator is physiological saline.
5. a kind of preparation method of many vesicles of the cladding D-pantothenyl aleohol as described in claims 1,2,3 or 4, its feature
It is to specifically include following steps：
1）Phosphatide, neutral lipid, cholesterol, organic solvent, hydrophilic emulsifier, D-pantothenyl aleohol, ammonia are weighed according to mass percent
Base acid, osmotic pressure regulator and deionized water；
2）Phosphatide, cholesterol and neutral lipid are dissolved in organic solvent, as organic phase；
3）The Freamine Ⅲ of 1 ~ 100mmol/L is configured, by D-pantothenyl aleohol, 30 ~ 60% Freamine Ⅲ, 30 ~ 60% osmotic pressure
Conditioning agent and the mixing of 30 ~ 60% deionized water, as interior water phase；
4）By step 3）Water is added in organic phase in middle gained, with homogeneous speed 7200~30000rpm homogeneous organic phase groups
Point, while interior water phase components are added thereto, 1~15min of homogeneous, w/o type colostric fluid is obtained；
5）By hydrophilic emulsifier, the Freamine Ⅲ of remaining content, the osmotic pressure regulator of remaining content and remaining content
The mixed solution of deionized water, as outer water phase；
6）With the outer water phase components of homogeneous 7200~30000rpm of speed homogeneous, while by step 4）Obtained w/o type colostric fluid group
Point be added thereto, after 1~15min of homogeneous, select ultrasonic power 20%~30%, ultrasound stops 5 seconds in 5 seconds, to emulsion ultrasound 1~
20min, to system, natural cooling is down to room temperature in the lump for stirring, is made W/O/W type multiple emulsions；
7）Configuration and step 3) in concentration identical Freamine Ⅲ, the amino acid that the multiple emulsion that will be obtained is scattered in configuration is molten
In liquid, wherein multiple emulsion and the volume ratio of Freamine Ⅲ is 5:1~1:5, be passed through nitrogen remove multiple emulsion in it is organic
Solvent, is obtained many vesicles suspension of cladding D-pantothenyl aleohol.
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Cited By (3)
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| CN114848591A (en) * | 2022-04-29 | 2022-08-05 | 浙江大学 | Preparation method of arginine polycystic liposome |
| CN115530359A (en) * | 2022-10-12 | 2022-12-30 | 广东岭南感恩生物科技有限公司 | Slow-release coagulant for hard bean curd and preparation method thereof |
| US11617706B2 (en) | 2020-06-29 | 2023-04-04 | The Procter & Gamble Company | Hair conditioning composition free of fatty alcohol |
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| CN103432009A (en) * | 2013-09-12 | 2013-12-11 | 广东轻工职业技术学院 | Whitening agent liposome coating micro-capsule composition as well as preparation method and application thereof |
| CN105581911A (en) * | 2014-11-27 | 2016-05-18 | 无限极（中国）有限公司 | Glabridin-cladded nano solid lipid carrier and preparation method and application thereof |
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| CN101780039A (en) * | 2010-03-05 | 2010-07-21 | 南京海陵中药制药工艺技术研究有限公司 | Tramadol multivesicular liposome and preparation method thereof |
| CN103432009A (en) * | 2013-09-12 | 2013-12-11 | 广东轻工职业技术学院 | Whitening agent liposome coating micro-capsule composition as well as preparation method and application thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US11617706B2 (en) | 2020-06-29 | 2023-04-04 | The Procter & Gamble Company | Hair conditioning composition free of fatty alcohol |
| US11969490B2 (en) | 2020-06-29 | 2024-04-30 | The Procter & Gamble Company | Hair conditioning composition with antimicrobial system |
| CN114848591A (en) * | 2022-04-29 | 2022-08-05 | 浙江大学 | Preparation method of arginine polycystic liposome |
| CN115530359A (en) * | 2022-10-12 | 2022-12-30 | 广东岭南感恩生物科技有限公司 | Slow-release coagulant for hard bean curd and preparation method thereof |
| CN115530359B (en) * | 2022-10-12 | 2024-03-19 | 广东岭南感恩生物科技有限公司 | Sustained-release coagulant for old bean curd and preparation method thereof |
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