CN105147991A - Traditional Chinese medicine combination for inhibiting melanin generation and preparation method and application thereof - Google Patents
Traditional Chinese medicine combination for inhibiting melanin generation and preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine combination for inhibiting melanin generation. The traditional Chinese medicine combination is prepared with, by weight, 15-35 parts of gingko leaf, 5-50 parts of mint leaf, 15-40 parts of raspberry, 10-35 parts of Hangzhou white chrysanthemum, 10-30 parts of fragrant solomonseal rhizome and 5-40 parts of dwarf lilyturf tuber. The traditional Chinese medicine combination is reasonable in composition, can realize synergistic effect, can efficiently remove oxygen radicals and inhibit tyrosinase activity, is ultraviolet-resistant, capable of accelerating renewal of skin superficial cells, improving skin immunity and delaying skin aging, and cosmetics prepared with the traditional Chinese medicine combination are stable in property has remarkable effect on whitening and brightening skin.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition, be specially Chinese medicine composition and preparation method thereof that a kind of check melanin formed and its application in cosmetics.
Background technology
Along with the increase at age, the pigmentation after brown patch, freckle and Exposure to Sunlight can produce, increases or not easily disappear on skin, and this has become the worry of middle advanced age layer people, particularly women.
Endocrine gland maincenter hypophysis in dye and human body has close ties.Hypophysis has two kinds of melanocytes to stimulate secreting hormone (MSH), i.e. α-MSH and β-MSH.MSH can make tyrosinase activity in melanocyte strengthen, the copper compound of tyrosine is made to become cuprous compound, strengthen epidermis cell and engulf melanin granule, and impel melanin granule to become the state of oxidation from reducing condition under ultraviolet radiation, cause cutaneous pigmentation.
The biochemical reaction occurred in above-mentioned melanocyte is: amino tyrosine generates DOPA quinone under the effect of tryrosinase, and DOPA quinone is transformed into the melanin of black under the Oxidation of enzyme or non-enzymatic through red pigments and colourless pigment.Therefore by check melanin cytoactive, restraint of tyrosinase is active, scavenging free radicals, reduces ultraviolet radiation, prevents the approach such as oxidation reaction generation, effectively can reduce melanin and be formed.
People, from restraint of tyrosinase activity, have developed multiple whitening agent at present, as ellagic acid, ascorbic acid, hydroquinone, kojic acid, Placenta extract and arbutin etc.The plant extract component reported has: the Pseudobulbus Bletillae (Rhizoma Bletillae), the Rhizoma Atractylodis Macrocephalae, Poria, Radix Angelicae Sinensis, Radix Glycyrrhizae, Rhizoma Chuanxiong, Fructus Hippophae, Radix Saposhnikoviae and Radix Bupleuri etc.
A lot of existing chemosynthesis agent, control melanic generation from the activity of restraint of tyrosinase, it is satisfactory in stability, effect etc., but side effect is obvious, and life-time service exists potential hazardness.
Summary of the invention
The present invention is directed to the defect of the tyrosinase inhibitor of existing chemosynthesis, providing a kind of is primary raw material with natural Chinese medicinal herb, has scavenging free radicals, the Chinese medicine composition that check melanin is formed.
The second object of the present invention is to provide the preparation method of the Chinese medicine composition that described check melanin is formed.
The third object of the present invention is to provide the application of the Chinese medicine composition that described check melanin is formed.
For solving above-mentioned technical problem, the present invention adopts following technical scheme:
The Chinese medicine composition that check melanin is formed, it is made up of the component comprising following parts by weight: Folium Ginkgo 15 ~ 35, mentha leave 5 ~ 50, Fructus Rubi 15 ~ 40, Flos Chrysanthemi 10 ~ 35, Rhizoma Polygonati Odorati 10 ~ 30, Radix Ophiopogonis 5 ~ 40.
Preferably, the Chinese medicine composition that described check melanin is formed, it is made up of the component comprising following parts by weight: Folium Ginkgo 35, mentha leave 5, Fructus Rubi 30, Flos Chrysanthemi 10, Rhizoma Polygonati Odorati 10, Radix Ophiopogonis 10.
Preferably, the Chinese medicine composition that described check melanin is formed, it is made up of the component comprising following parts by weight: Folium Ginkgo 15, mentha leave 5, Fructus Rubi 40, Flos Chrysanthemi 10, Rhizoma Polygonati Odorati 20, Radix Ophiopogonis 10.
Preferably, the Chinese medicine composition that described check melanin is formed, it is made up of the component comprising following parts by weight: Folium Ginkgo 25, mentha leave 5, Fructus Rubi 25, Flos Chrysanthemi 20, Rhizoma Polygonati Odorati 20, Radix Ophiopogonis 5.
Other desired functional component can also be added in the Chinese medicine composition that check melanin of the present invention is formed, as: deionized water, nourishing additive agent, whitening additive, moisturizing additive, skin sore additive of dispelling, anti-ultraviolet additive, defying age additive, cosmetic colors, antiseptic, antioxidant and essence and flavoring agent etc.
The Chinese medicine composition that described check melanin is formed adopts following preparation method:
(1) medical material pretreatment: respectively Folium Ginkgo, mentha leave, Fructus Rubi, Flos Chrysanthemi, Rhizoma Polygonati Odorati and Radix Ophiopogonis powder are broken into 80 ~ 200 object powder, then mix homogeneously;
(2) homogenizing extracts: powder is placed in high pressure homogenizer, adds the Extraction solvent of medical material gross weight 5 ~ 50 times, high pressure homogenization method 5 ~ 30min, extraction pressure is 10 ~ 70Mpa;
(3) concentrated and purified: by the micro-filtrate membrane filtration in 0.2 ~ 0.5 μm, extracting solution aperture, 200 ~ 2000ml when membrance concentration is extremely using 100g medicinal powder as standard under 5 ~ 30bar pressure through reverse osmosis membrane by filtrate, the macroporous resin column being D101 or AB-8 by resin type on concentrated solution number be washed to colourless after, eluent is eluted to again colourless with the alcoholic solution that volume by volume concentration is 20 ~ 80%, and collect ethanol elution, with the microporous filter in 0.2 ~ 0.5 μm, aperture after eluent activated carbon decolorizing, obtain medicinal liquid;
(4) concentrate drying: medicinal liquid membrance concentration being reclaimed mixture weight percentage ratio in ethanol to medicinal liquid and step (1) is 1: 2 ~ 1: 5, by medicinal liquid at 50 ~ 70 DEG C of vacuum dryings, the Chinese medicine composition that the melanin that is inhibited is formed.
Described Extraction solvent is water, volume by volume concentration is 90 ~ 95% alcoholic solution and volume by volume concentration is any one in 30 ~ 80% alcoholic solution.
The Chinese medicine composition that described check melanin is formed may be used in skin-lightening cosmetic, and its addition is 0.1 ~ 5%.
Preferably, the addition that the Chinese medicine composition that described check melanin is formed is used in skin-lightening cosmetic is 1 ~ 3%.
Compared with prior art, of the present invention have following beneficial effect: reasonable component formula, can Synergistic, can potent scavenging activated oxygen and restraint of tyrosinase active, the cosmetic property prepared is stablized, the bright skin Be very effective of whitening.
Accompanying drawing explanation
Fig. 1 is that check melanin of the present invention forms effect assessment result of the test column schematic diagram (wherein, a is the butanediol group of 20%, and b is the Chinese medicine composition group that check melanin is formed).
Specific embodiment
In order to set forth content of the present invention better, be described with some preferably specific embodiments below.But these specific embodiments are just in order to illustrate content of the present invention, instead of limit content of the present invention.Principle explanation
The Chinese medicine composition that check melanin of the present invention is formed is theoretical with skin-whitening skin care in conjunction with the side's of sending principle of Chinese prescription monarch, and the selection of realization amount skin shoots the arrow at the target, and balances whitening and skin care.
Folium Ginkgo and Fructus Rubi are monarch drug.Folium Ginkgo nature and flavor are sweet, bitter, puckery, and flat, GUIXIN, lung meridian, containing a large amount of flavone and ginkgoic acid; Fructus Rubi nature and flavor are sweet, sour, and temperature, returns liver, kidney, urinary bladder channel, containing a large amount of gallic acids, sitosterol and Fructus Rubi acid; The mentioned component of Folium Ginkgo and Fructus Rubi has significant oxidation resistance, can grow by check melanin, has the beauty functions of extraordinary preserve youthful looks foster skin and whitening and speckle dispelling.
Rhizoma Polygonati Odorati and Flos Chrysanthemi are ministerial drug.Rhizoma Polygonati Odorati nature and flavor are sweet, are slightly cold, and attach to the lung and stomach meridians, and containing a large amount of mucopolysaccharides and steroid glycoside, mentioned component, except moisturizing, scavenging free radicals, can also improve Hemolysin level, improves the breeder reaction of splenocyte to con A; Flos Chrysanthemi contains a large amount of volatile oil, chrysanthemum glycoside and adenine, and mentioned component has calm antiallergic and the effect of refreshing body of releiving.
Radix Ophiopogonis is adjuvant drug, and sweet, the micro-hardship of nature and flavor, is slightly cold, GUIXIN, lung, stomach warp, containing multiple steroid saponin and chromocor compound, can effectively generate and delay antibody and disappear by enhancing antibody, improve immunization of cell.
Herba Menthae for making medicine, coordinating the actions of various ingredients in a prescription, nature and flavor are pungent, cool, return lung meridian, Liver Channel, containing abundant menthol, can antiviral, sterilization and promotion ingredient osmotic absorption.
Share under the proportioning that all medicines are mentioned in the present invention above, can Synergistic, qi-supplementing, blood-engendering, smoothing skin, conditioning viscera, Yin Yang balancing, control the bright skin of whitening this.
The potent scavenging free radicals of Chinese medicine composition energy that check melanin of the present invention is formed, restraint of tyrosinase are active, uvioresistant, reduce melanic generation, accelerate skin surface cell to upgrade, strengthen skin immunization power and delaying skin aging, make an addition in skin-lightening cosmetic, whitening bright skin effect is remarkable.
Embodiment 1
The Chinese medicine composition that check melanin is formed, be made up of the parts by weight Chinese crude drug component in following formula table:
| Component | Parts by weight |
| Folium Ginkgo | 35 |
| Mentha leave | 5 |
| Fructus Rubi | 30 |
| Flos Chrysanthemi | 10 |
| Rhizoma Polygonati Odorati | 10 |
| Radix Ophiopogonis | 10 |
Preparation method is as follows:
Chinese crude drug component in formula table is pulverized, cross mix homogeneously after 80 mesh sieves, obtain medicinal material mixture, add the pure water of medicinal material mixture gross weight 6 times, high pressure homogenization method 30min, extract pressure 50Mpa, merge extractive liquid, with the microporous filter in 0.3 μm, aperture, by filtrate membrance concentration to the 10-15% of original volume, the macroporous resin column that upper resin type number is D101 or AB-8 be washed to colourless after, be that 20% ethanol water eluting is colourless to eluent with volume by volume concentration again, and collect ethanol elution, eluent 5% (W/V) activated carbon decolorizing, with the microporous filter in 0.3 μm, aperture, it is 1: 2 to medicinal liquid and medicinal material mixture ratio that filtrate membrance concentration reclaims ethanol, last 70 DEG C of vacuum dryings.
Embodiment 2
The Chinese medicine composition that check melanin is formed, be made up of the parts by weight Chinese crude drug component in following formula table:
| Component | Parts by weight |
| Folium Ginkgo | 15 |
| Mentha leave | 5 |
| Fructus Rubi | 40 |
| Flos Chrysanthemi | 10 |
| Rhizoma Polygonati Odorati | 20 |
| Radix Ophiopogonis | 10 |
Preparation method is as follows:
Chinese crude drug component in formula table is pulverized, cross mix homogeneously after 100 mesh sieves, obtain medicinal material mixture, add 30% ethanol water of medicinal material mixture gross weight 12 times, high pressure homogenization method 15min, extract pressure 70Mpa, merge extractive liquid, with the microporous filter in 0.2 μm, aperture, filtrate membrance concentration is reclaimed ethanol extremely without after alcohol taste, add 2 times of water gaging dilutions, the macroporous resin column that upper resin type number is D101 or AB-8 be washed to colourless after, be that 40% alcoholic solution is eluted to eluent with volume by volume concentration more colourless, and collect ethanol elution, eluent 8% (W/V) activated carbon decolorizing, with the microporous filter in 0.2 μm, aperture, it is 1: 4 to medicinal liquid and medicinal material mixture ratio that filtrate membrance concentration reclaims ethanol, last 60 DEG C of vacuum dryings.
Embodiment 3
The Chinese medicine composition that check melanin is formed, be made up of the parts by weight Chinese crude drug component in following formula table:
| Component | Parts by weight |
| Folium Ginkgo | 25 |
| Mentha leave | 5 |
| Fructus Rubi | 25 |
| Flos Chrysanthemi | 20 |
| Rhizoma Polygonati Odorati | 20 |
| Radix Ophiopogonis | 5 |
Preparation method is as follows:
Chinese crude drug component in formula table is pulverized, cross mix homogeneously after 120 mesh sieves, obtain medicinal material mixture, add 70% ethanol water of medicinal material mixture gross weight 20 times, high pressure homogenization method 10min, extract pressure 30Mpa, merge extractive liquid, with the microporous filter in 0.5 μm, aperture, filtrate membrance concentration is reclaimed ethanol extremely without after alcohol taste, add 2 times of water gaging dilutions, the macroporous resin column that upper resin type number is D101 or AB-8 be washed to colourless after, be that 70% alcoholic solution is eluted to eluent with volume by volume concentration more colourless, and collect ethanol elution, eluent 10% (W/V) activated carbon decolorizing, with the microporous filter in 0.5 μm, aperture, it is 1: 5 to medicinal liquid and medicinal material mixture ratio that filtrate membrance concentration reclaims ethanol, last 60 DEG C of vacuum dryings.
Embodiment 4
The Chinese medicine composition that check melanin is formed, be made up of the parts by weight Chinese crude drug component in following formula table:
| Component | Parts by weight |
| Folium Ginkgo | 15 |
| Mentha leave | 5 |
| Fructus Rubi | 15 |
| Flos Chrysanthemi | 35 |
| Rhizoma Polygonati Odorati | 30 |
| Radix Ophiopogonis | 10 |
Preparation method is as follows:
(1) medical material pretreatment: the Chinese crude drug component Folium Ginkgo respectively just in formula table, mentha leave, Fructus Rubi, Flos Chrysanthemi, Rhizoma Polygonati Odorati and Radix Ophiopogonis powder are broken into 160 object powder, then mix homogeneously;
(2) homogenizing extracts: powder is placed in high pressure homogenizer, adds the ethanol of 90 ~ 95% of component weight 5 times, high pressure homogenization method 5min, extracts pressure 20Mpa;
(3) concentrated and purified: by the micro-filtrate membrane filtration in 0.4 μm, extracting solution aperture, by filtrate through reverse osmosis membrane under 30bar pressure membrance concentration to 200ml, the macroporous resin column being D101 or AB-8 by resin type on concentrated solution number be washed to colourless after, eluent is eluted to again colourless with volume by volume concentration 30% alcoholic solution, and collect ethanol elution, eluent uses the microporous filter of 0.4 μm, aperture with after 1% (W/V) activated carbon decolorizing, obtains medicinal liquid;
(4) concentrate drying: medicinal liquid membrance concentration being reclaimed mixture weight percentage ratio in ethanol to medicinal liquid and step (1) is 1: 3, by medicinal liquid at 50 DEG C of vacuum dryings, obtains Chinese medicine composition extract.
Embodiment 5
The Chinese medicine composition that check melanin is formed, be made up of the parts by weight Chinese crude drug component in following formula table:
| Component | Parts by weight |
| Folium Ginkgo | 15 |
| Mentha leave | 50 |
| Fructus Rubi | 20 |
| Flos Chrysanthemi | 10 |
| Rhizoma Polygonati Odorati | 10 |
| Radix Ophiopogonis | 30 |
Preparation method is as follows:
(1) medical material pretreatment: the Chinese crude drug component Folium Ginkgo respectively just in formula table, mentha leave, Fructus Rubi, Flos Chrysanthemi, Rhizoma Polygonati Odorati and Radix Ophiopogonis powder are broken into 200 object powder, then mix homogeneously;
(2) homogenizing extracts: powder is placed in high pressure homogenizer, adds 50% ethanol water of component weight 10 times, high pressure homogenization method 20min, extracts pressure 40Mpa;
(3) concentrated and purified: by the micro-filtrate membrane filtration in 0.5 μm, extracting solution aperture, by filtrate through reverse osmosis membrane under 20bar pressure membrance concentration to 500ml, the macroporous resin column being D101 or AB-8 by resin type on concentrated solution number be washed to colourless after, eluent is eluted to again colourless with volume by volume concentration 50% alcoholic solution, and collect ethanol elution, eluent uses the microporous filter of 0.5 μm, aperture with after 6% (W/V) activated carbon decolorizing, obtains medicinal liquid;
(4) concentrate drying: medicinal liquid membrance concentration being reclaimed mixture weight percentage ratio in ethanol to medicinal liquid and step (1) is 1: 5, by medicinal liquid at 50 DEG C of vacuum dryings, obtains Chinese medicine composition extract.
Embodiment 6
The Chinese medicine composition that check melanin is formed, be made up of the parts by weight Chinese crude drug component in following formula table:
| Component | Parts by weight |
| Folium Ginkgo | 30 |
| Mentha leave | 20 |
| Fructus Rubi | 20 |
| Flos Chrysanthemi | 25 |
| Rhizoma Polygonati Odorati | 20 |
| Radix Ophiopogonis | 25 |
Preparation method is as follows:
(1) medical material pretreatment: the Chinese crude drug component Folium Ginkgo respectively just in formula table, mentha leave, Fructus Rubi, Flos Chrysanthemi, Rhizoma Polygonati Odorati and Radix Ophiopogonis powder are broken into 140 object powder, then mix homogeneously;
(2) homogenizing extracts: powder is placed in high pressure homogenizer, adds 80% ethanol water of component weight 30 times, high pressure homogenization method 25min, extracts pressure 10Mpa;
(3) concentrated and purified: by the micro-filtrate membrane filtration in 0.2 μm, extracting solution aperture, by filtrate through reverse osmosis membrane under 10bar pressure membrance concentration to 800ml, the macroporous resin column being D101 or AB-8 by resin type on concentrated solution number be washed to colourless after, eluent is eluted to again colourless with the alcoholic solution that volume by volume concentration is 60%, and collect ethanol elution, the microporous filter of eluent with 0.2 μm, aperture after 3% (W/V) activated carbon decolorizing, obtains medicinal liquid;
(4) concentrate drying: medicinal liquid membrance concentration being reclaimed mixture weight percentage ratio in ethanol to medicinal liquid and step (1) is 1: 4, by medicinal liquid at 70 DEG C of vacuum dryings, obtains Chinese medicine composition extract.
Embodiment 7
The Chinese medicine composition that check melanin is formed, be made up of the parts by weight Chinese crude drug component in following formula table:
| Component | Parts by weight |
| Folium Ginkgo | 20 |
| Mentha leave | 25 |
| Fructus Rubi | 30 |
| Flos Chrysanthemi | 25 |
| Rhizoma Polygonati Odorati | 10 |
| Radix Ophiopogonis | 40 |
Preparation method is as follows:
(1) medical material pretreatment: respectively Folium Ginkgo, mentha leave, Fructus Rubi, Flos Chrysanthemi, Rhizoma Polygonati Odorati and Radix Ophiopogonis powder are broken into 180 object powder, then mix homogeneously;
(2) homogenizing extracts: powder is placed in high pressure homogenizer, adds 40 ~ 60% ethanol waters of medical material gross weight 15 ~ 50 times, high pressure homogenization method 8 ~ 28min, extracts pressure 60Mpa;
(3) concentrated and purified: by the micro-filtrate membrane filtration in 0.3 μm, extracting solution aperture, by filtrate through reverse osmosis membrane membrance concentration to 1000 ~ 2000ml under 5bar pressure, the macroporous resin column being D101 or AB-8 by resin type on concentrated solution number be washed to colourless after, eluent is eluted to again colourless with the alcoholic solution that volume by volume concentration is 80%, and collect ethanol elution, the microporous filter of eluent with 0.3 μm, aperture after 2 ~ 9% (W/V) activated carbon decolorizing, obtains medicinal liquid;
(4) concentrate drying: medicinal liquid membrance concentration being reclaimed mixture weight percentage ratio in ethanol to medicinal liquid and step (1) is 1: 2, by medicinal liquid at 60 DEG C of vacuum dryings, the Chinese medicine composition that the melanin that is inhibited is formed.
Embodiment 8
The body whitening crème that check melanin is formed, be made up of the component of following mass percent:
Preparation method is as follows:
(1) mixture that component A stereth-2, stereth-21, light liquid petrolatum, cetearyl alcohol, distillation monoglyceride, vaseline, iso-octyl palmitate, caprylic/capric triglyceride, polydimethylsiloxane, isopropyl myristate, methyl hydroxybenzoate and oxybenzene third fat form being heated to 70 DEG C dissolves completely;
(2) carbopol 940 in B component is used deionized water dissolving in advance, hyaluronate sodium uses deionized water dissolving again with after glycerol dispersion in advance, is stirred to and dissolves completely, be heated to 70 DEG C;
(3) while stirring the mixture of above-mentioned component A is added in the mixture of B component EDTA-2Na, propylene glycol, Chinese medicine composition, allantoin and the carbopol 940 processed through step (2) that check melanin is formed and hyaluronate sodium composition, 70 DEG C are stirred 1min, homogenizing 1min, continues 10min;
(4) mixture of component C aminomethyl propanol and essence composition is added in the mixture obtained to step (3) when being cooled to 50 DEG C, homogenizing 1min;
(5) Fruit storage is carried out after being dissolved completely by the mixture that step (4) obtains.
Embodiment 9
The Whitening sunscreen cream that check melanin is formed, be made up of the component of following mass percent:
Preparation method is as follows:
(1) mixture that component A octocrilene, ethylhexyl methoxy cinnamate, Dimethicodiethylbenzalmalonate, 4-methyl benzylidene camphor, BEMT, C12-15 alcohol benzoate, dicaprylyl carbonate, lauryl KF-6028, sorbitan sesquioleate, silicone oil, silica gel, vitamin E and bisabolol form being heated to 70 DEG C dissolves completely;
(2) hyaluronate sodium in B component is used deionized water dissolving again with after glycerol dispersion in advance, other compositions are stirred to and dissolve completely, are heated to 70 DEG C;
(3) while stirring the mixture of above-mentioned component A is added B component EDTA-2Na, in Chinese medicine composition that check melanin is formed, allantoin, DL-Panthenol, Sal, glycerol, butanediol, sodium hydroxide, Phenylbenzimidazolesulfonic acid and the mixture of hyaluronate sodium composition that processes through step (2), 70 DEG C are stirred 1min, homogenizing 1min, continues 10min;
(4) mixture of component C Methyl p-hydroxybenzoate, propylparaben, de-an aromatic plant metioned in ancient books ethanol, phenoxyethanol and essence composition is added in the mixture obtained to step (3) when being cooled to 50 DEG C, homogenizing 1min;
(5) Fruit storage is carried out after being dissolved completely by the mixture that step (4) obtains.
Embodiment 10
The whitening breast that a kind of check melanin is formed, be made up of the component of following mass percent:
Preparation method is as follows:
(1) mixture that component A stereth-2, stereth-21, light liquid petrolatum, cetearyl alcohol, distillation monoglyceride, vaseline, iso-octyl palmitate, caprylic/capric triglyceride, polydimethylsiloxane, isopropyl myristate, methyl hydroxybenzoate and oxybenzene third fat form being heated to 70 DEG C dissolves completely;
(2) carbopol 940 in B component is used deionized water dissolving in advance, hyaluronate sodium uses deionized water dissolving again with after glycerol dispersion in advance, is stirred to and dissolves completely, be heated to 70 DEG C;
(3) Chinese medicine composition, allantoin and the carbopol 940 processed through step (2) that B component EDTA-2Na, propylene glycol, check melanin are formed while stirring and the mixture of hyaluronate sodium composition add in the mixture of above-mentioned component A, 70 DEG C are stirred 1min, homogenizing 1min, continues 10min;
(4) mixture of component C aminomethyl propanol and essence composition is added in the mixture obtained to step (3) when being cooled to 50 DEG C, homogenizing 1min;
(5) Fruit storage is carried out after being dissolved completely by the mixture that step (4) obtains.
Embodiment 11
The whitening water that check melanin is formed, be made up of the component of following mass percent:
Preparation method is as follows:
(1) butanediol, xanthan gum, hyaluronate sodium, glycerol, deionized water, carbomer, propylene glycol, methyl ester antiseptic and propyl ester antiseptic are disperseed in advance;
(2) Chinese medicine composition component A deionized water, EDTA-2Na, allantoin, trehalose, check melanin formed and hydroxyethyl-cellulose are stirred to and dissolve completely, add butanediol, xanthan gum, hyaluronate sodium, glycerol, deionized water, carbomer, propylene glycol, methyl ester antiseptic, propyl ester antiseptic, outstanding agate antiseptic and aminomethyl propanol while stirring successively;
(3) Fruit storage is carried out after being dissolved completely by the mixture that step (2) obtains.
Needs illustrate, mass percent can be adjusted to " 0.1 ~ 1% " or " 3 ~ 5% " according to its embody rule situation in cosmetics by the addition of the Chinese medicine composition of the check melanin formation of embodiment 8 to 11.
Scavenging free radicals effect test
The Chinese medicine composition that the check melanin obtained by above-described embodiment 1, embodiment 2, embodiment 3 is formed, carry out scavenging free radicals effect test, test method is as follows:
The DPPH solution 1mL getting mass concentration 0.02%, in small test tube, adds test specimen, then adds methanol to 5mL, mixing, leaves standstill after 30 minutes, surveys 517nm place light absorption value A.
The DPPH solution 1mL getting mass concentration 0.02%, in small test tube, adds methanol to 5mL, mixing, leaves standstill after 30 minutes, surveys 517nm place light absorption value A
0.Clearance rate=(A
0-A)/A
0* 100%
Result of the test sees the following form:
Experimentally result is known, and the Chinese medicine composition of the check melanin formation that embodiment 1,2,3 is obtained is to DPPH free radical scavenging successful, and elimination effect reaches the 1/3-1/4 of the ascorbic acid elimination effect of purity 99%.
Shown by test, other embodiments also have similar effect.
Therefore, the Chinese medicine composition that check melanin of the present invention is formed can reach well removes DPPH free radical effect.
Restraint of tyrosinase activity test
The Chinese medicine composition that the check melanin obtained by above-described embodiment 1, embodiment 2, embodiment 3 is formed, carry out the test alive of suppression tyrosine two phenolase, test method is as follows:
According to following table application of sample, first do not add tryrosinase, solution adds tryrosinase after being placed in 37 DEG C of water-bath constant temperature 10min, then after solution being placed in 37 DEG C of water-bath constant temperature 5min, under 475nm, measures its absorbance rapidly.
Computing formula: inhibitory activity against tyrosinase=[1-(A
2/ A
1)] × 100%
A in formula
1for the absorbance of blank; A
2for the absorbance of test specimen.
Result of the test sees the following form:
Experimentally result is known, and the Chinese medicine composition of the check melanin formation that embodiment 1,2,3 is obtained is obvious to tyrosine o-diphenolase activity inhibition, close to the inhibition of purity 99% kojic acid.
Shown by test, other embodiments also have similar effect.
Therefore, the Chinese medicine composition that check melanin of the present invention is formed can reach good tyrosinase activity inhibition.
Check melanin forms effect assessment test
By the Chinese medicine composition that check melanin obtained for embodiment 3 is formed, be dissolved in 20% butanediol solution, the Chinese medicine composition solution that the check melanin being made into 3% is formed, as test sample; Using 20% butanediol solution as blank.
Test according to " cosmetics health specification " (version in 2007), select 30 (10 male, 20 women) healthy, without the volunteer of cosmetic allergic contact dermatitis history, carry out dermal melanin detection.Dermal melanin content detection the results are shown in shown in accompanying drawing 1.
Testing result shows, the Chinese medicine composition that the obtained check melanin of life-time service embodiment 3 is formed can effectively slow down B16 cell speed, improves the colour of skin and non-stimulated to skin.
Shown by test, other embodiments also have similar effect.
Therefore, the Chinese medicine composition that check melanin of the present invention is formed can effectively slow down B16 cell speed, improves the colour of skin and non-stimulated to skin.
Above-described embodiment is the present invention's preferably embodiment; but embodiments of the present invention are not restricted to the described embodiments; change, the modification done under other any does not deviate from spirit of the present invention and principle, substitute, combine, simplify; all should be equivalent replacement mode, be included within protection scope of the present invention.
Claims (8)
1. a Chinese medicine composition for check melanin formation, is characterized in that: be made up of the component comprising following parts by weight: Folium Ginkgo 15 ~ 35, mentha leave 5 ~ 50, Fructus Rubi 15 ~ 40, Flos Chrysanthemi 10 ~ 35, Rhizoma Polygonati Odorati 10 ~ 30, Radix Ophiopogonis 5 ~ 40.
2. the Chinese medicine composition of check melanin formation according to claim 1, is characterized in that, be made up of the component comprising following parts by weight: Folium Ginkgo 35, mentha leave 5, Fructus Rubi 30, Flos Chrysanthemi 10, Rhizoma Polygonati Odorati 10, Radix Ophiopogonis 10.
3. the Chinese medicine composition of check melanin formation according to claim 1, is characterized in that, be made up of the component comprising following parts by weight: Folium Ginkgo 15, mentha leave 5, Fructus Rubi 40, Flos Chrysanthemi 10, Rhizoma Polygonati Odorati 20, Radix Ophiopogonis 10.
4. the Chinese medicine composition of check melanin formation according to claim 1, is characterized in that, be made up of the component comprising following parts by weight: Folium Ginkgo 25, mentha leave 5, Fructus Rubi 25, Flos Chrysanthemi 20, Rhizoma Polygonati Odorati 20, Radix Ophiopogonis 5.
5. the preparation method of the Chinese medicine composition of check melanin formation according to claim 1, it is characterized in that, the step comprised is as follows:
(1) medical material pretreatment: respectively Folium Ginkgo, mentha leave, Fructus Rubi, Flos Chrysanthemi, Rhizoma Polygonati Odorati and Radix Ophiopogonis are pulverized, then mix homogeneously;
(2) homogenizing extracts: powder is placed in high pressure homogenizer, adds the Extraction solvent of medical material gross weight 5 ~ 50 times, high pressure homogenization method;
(3) concentrated and purified: by extracting solution micro-filtrate membrane filtration, by filtrate through reverse osmosis membrane membrance concentration to 200 ~ 2000ml under 5 ~ 30bar pressure, the macroporous resin column being D101 or AB-8 by resin type on concentrated solution number be washed to colourless after, eluent is eluted to again colourless with the alcoholic solution that volume by volume concentration is 20 ~ 80%, and collect ethanol elution, with microporous filter after eluent activated carbon decolorizing, obtain medicinal liquid;
(4) concentrate drying: medicinal liquid membrance concentration being reclaimed mixture weight percentage ratio in ethanol to medicinal liquid and step (1) is 1: 2 ~ 1: 5, by medicinal liquid at 50 ~ 70 DEG C of vacuum dryings.
6. the Chinese medicine composition that formed of check melanin according to claim 5, is characterized in that: the Extraction solvent of described step (2) is water, volume by volume concentration 90 ~ 95% alcoholic solution and volume by volume concentration are any one in 30 ~ 80% alcoholic solution.
7. the Chinese medicine composition of check melanin formation according to claim 1, it is characterized in that: in cosmetics, its addition is 0.1 ~ 5%.
8. the Chinese medicine composition of check melanin formation according to claim 1, it is characterized in that: in cosmetics, its addition is 1 ~ 3%.
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