CN105130583A - Preparation method of matrix for culturing straw mushrooms - Google Patents
Preparation method of matrix for culturing straw mushrooms Download PDFInfo
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- CN105130583A CN105130583A CN201510461855.9A CN201510461855A CN105130583A CN 105130583 A CN105130583 A CN 105130583A CN 201510461855 A CN201510461855 A CN 201510461855A CN 105130583 A CN105130583 A CN 105130583A
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- straw
- mass content
- culture medium
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- wood fragments
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Abstract
The invention discloses a preparation method of a matrix for culturing straw mushrooms. The matrix is prepared by mixing the following components in percentage by weight: 30 to 50% of secondary wood chips, which have been hydrolyzed by laccase, 20 to 40% of minced straw, 5 to 10% of secondary minced straw, which has been hydrolyzed by cellulase, 1 to 5% of calcium superphosphate, and 1 to 5% of gypsum powder. The waste straws can be fully utilized, and the mushroom cultured by the matrix has a fresh and crisp taste.
Description
Technical field
The invention belongs to mushroom cultivation field.
Background technology
In mushroom cultivating process, the main two problems one considered is the level of growth of mushroom itself, another is the control of miscellaneous bacteria and insect pest in mushroom cultivating process, the control of these two aspects and balance are except relying on growth conditions, as outside the regulating and control of temperature, humidity etc., also depend on the selection of culture medium, not only if culture medium is beneficial to the growth of mushroom itself but also pole is beneficial to the breeding of miscellaneous bacteria and insect, then not favourable to the cultivation of mushroom.
Summary of the invention
The object of the present invention is to provide one can both meet straw mushroom well to grow, obtain a mushroom straw mushroom that large stem stalk is little, mouthfeel is crisp and refreshing, the culture medium that can effectively suppress varied bacteria growing to be bred again.
Technical scheme of the present invention is as follows: the preparation method of the culture medium of a kind of straw mushroom, comprises the following steps:
1) by mass content be 30 ~ 50% wood fragments bits carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, at 30 ~ 40 DEG C, add laccase reactions 5 ~ 8h, obtain secondary wood fragments bits, this process is aseptically carried out;
2) by mass content be 5% ~ 10% crushing straw carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, add at 30 ~ 40 DEG C cellulase reaction 6 ~ 12h, obtain secondary crushing straw, this process is aseptically carried out;
3) by mass content be 20 ~ 40% crushing straw, mass content be 1% ~ 5% calcium superphosphate and mass content be 1% ~ 5% terra alba carry out high-temperature sterilization, thereafter to consider to be worth doing with described secondary wood fragments and described secondary crushing straw mixes mutually, namely obtain culture medium, mixing process is aseptically carried out.
Its preferred embodiment is: described cellulase be selected from circumscribed beta-glucanase, Endo-β-glucanase and beta-glucosidase one or more.
Cellulase herein also can select the complex class cellulase of commercial type.
Its another preferred embodiment is: described crushing straw is the crushed material of corn or wheat stalk.Its another preferred embodiment is: described wood fragments bits are the limb crushed material of fruits trees.
The trees of growth fruit that refer to of said fruits trees herein, as pear tree, Japanese plum, peach, apple tree, apricot etc.
Usefulness of the present invention is:
1) Appropriate application waste straw, is beneficial to circulation and the regeneration of resource;
2) culture medium prepared can grow and obtain the crisp and refreshing straw mushroom of mouthfeel;
3) culture medium prepared can suppress the growth of other miscellaneous bacteria and insect, obtains the straw mushroom of good quality.
Embodiment
Embodiment 1
1) by mass content be 50% apple tree wood fragments bits carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 6, at 35 DEG C, add thereafter quality is obtain secondary wood fragments bits after the laccase reactions 8h of wood fragments bits quality 8%;
2) by mass content be 10% wheat crushing straw carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 6, at 30 DEG C, add thereafter quality is obtain secondary crushing straw after the compound cellulose enzyme reaction 8h of crushing straw quality 10%;
3) by mass content be 34% wheat crushing straw, mass content be 1% calcium superphosphate, mass content be 5% terra alba carry out high-temperature sterilization, to consider to be worth doing with above-mentioned secondary wood fragments thereafter and secondary crushing straw fully mixes, namely obtain culture medium;
Said process all aseptically carries out.
Embodiment 2
1) by mass content be 40% pear tree wood fragments bits carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 4, at 40 DEG C, add thereafter quality is obtain secondary wood fragments bits after the laccase reactions 8h of wood fragments bits quality 8%;
2) by mass content be 10% corn crushing straw carry out high-temperature sterilization after add dilute acetic acid solution and regulate its pH value to be 5, at 35 DEG C, add thereafter quality is obtain secondary crushing straw after the circumscribed beta-glucan enzyme reaction 8h of crushing straw quality 7%;
3) by mass content be 40% corn crushing straw, mass content be 5% calcium superphosphate, mass content be 5% terra alba carry out high-temperature sterilization, thereafter to consider to be worth doing with above-mentioned secondary wood fragments and secondary crushing straw fully mixes, namely obtain culture medium, this process is aseptically carried out.
Claims (4)
1. a preparation method for the culture medium of straw mushroom, is characterized in that: comprise the following steps:
1) by mass content be 30 ~ 50% wood fragments bits carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, at 30 ~ 40 DEG C, add laccase reactions 5 ~ 8h, obtain secondary wood fragments bits, this process is aseptically carried out;
2) by mass content be 5% ~ 10% crushing straw carry out high-temperature sterilization, thereafter use dilute acetic acid solution regulate its pH value to be 4 ~ 6, add at 30 ~ 40 DEG C cellulase reaction 6 ~ 12h, obtain secondary crushing straw, this process is aseptically carried out;
3) by mass content be 20 ~ 40% crushing straw, mass content be 1% ~ 5% calcium superphosphate and mass content be 1% ~ 5% terra alba carry out high-temperature sterilization, thereafter to consider to be worth doing with described secondary wood fragments and described secondary crushing straw mixes mutually, namely obtain culture medium, mixing process is aseptically carried out.
2. the preparation method of the culture medium of straw mushroom according to claim 1, is characterized in that: described cellulase be selected from circumscribed beta-glucanase, Endo-β-glucanase and beta-glucosidase one or more.
3. the preparation method of the culture medium of straw mushroom according to claim 1, is characterized in that: described crushing straw is the crushed material of corn or wheat stalk.
4. the preparation method of the culture medium of straw mushroom according to claim 1, is characterized in that: described wood fragments bits are the limb crushed material of fruits trees.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510461855.9A CN105130583A (en) | 2015-07-31 | 2015-07-31 | Preparation method of matrix for culturing straw mushrooms |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510461855.9A CN105130583A (en) | 2015-07-31 | 2015-07-31 | Preparation method of matrix for culturing straw mushrooms |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105130583A true CN105130583A (en) | 2015-12-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510461855.9A Pending CN105130583A (en) | 2015-07-31 | 2015-07-31 | Preparation method of matrix for culturing straw mushrooms |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974436A (en) * | 2010-09-21 | 2011-02-16 | 中国农业科学院农业资源与农业区划研究所 | Lignocellulose degrading bacteria and application thereof |
| CN103497018A (en) * | 2013-08-29 | 2014-01-08 | 合肥市潜溪山庄农业生态园有限公司 | Needle mushroom cultivation matrix formula and its preparation method |
| CN104541972A (en) * | 2014-12-31 | 2015-04-29 | 安徽丰原发酵技术工程研究有限公司 | Method for cultivating edible fungi through agricultural straws |
-
2015
- 2015-07-31 CN CN201510461855.9A patent/CN105130583A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101974436A (en) * | 2010-09-21 | 2011-02-16 | 中国农业科学院农业资源与农业区划研究所 | Lignocellulose degrading bacteria and application thereof |
| CN103497018A (en) * | 2013-08-29 | 2014-01-08 | 合肥市潜溪山庄农业生态园有限公司 | Needle mushroom cultivation matrix formula and its preparation method |
| CN104541972A (en) * | 2014-12-31 | 2015-04-29 | 安徽丰原发酵技术工程研究有限公司 | Method for cultivating edible fungi through agricultural straws |
Non-Patent Citations (3)
| Title |
|---|
| 王贺祥，刘庆洪主编: "《食用菌栽培手册》", 31 January 2015 * |
| 申进文编著: "《食用菌生产技术大全》", 31 January 2014 * |
| 赵金海主编: "《生物化学》", 31 January 2013 * |
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Application publication date: 20151209 |
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| RJ01 | Rejection of invention patent application after publication |