CN105901040A - Enzyme-catalyzed seed germination accelerating agent - Google Patents

Enzyme-catalyzed seed germination accelerating agent Download PDF

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Publication number
CN105901040A
CN105901040A CN201610354559.3A CN201610354559A CN105901040A CN 105901040 A CN105901040 A CN 105901040A CN 201610354559 A CN201610354559 A CN 201610354559A CN 105901040 A CN105901040 A CN 105901040A
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parts
agent
seed
water
fresh
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CN201610354559.3A
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Chinese (zh)
Inventor
周承东
程圆
李德琴
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Anhui Huahe Seed Industry Co Ltd
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Anhui Huahe Seed Industry Co Ltd
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Priority to CN201610354559.3A priority Critical patent/CN105901040A/en
Publication of CN105901040A publication Critical patent/CN105901040A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02Acyclic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/42Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing within the same carbon skeleton a carboxylic group or a thio analogue, or a derivative thereof, and a carbon atom having only two bonds to hetero atoms with at the most one bond to halogen, e.g. keto-carboxylic acids
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N61/00Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/44Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Dentistry (AREA)
  • Plant Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Virology (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

The invention discloses an enzyme-catalyzed seed germination accelerating agent. The enzyme-catalyzed seed germination accelerating agent is prepared from the following raw materials in parts by weight: 26-28 parts of corn protein powder, 9-11 parts of vinegar dregs, 2-3 parts of xylitol, 0.6-0.8 part of acetylsalicylic acid, 0.3-0.4 part of barium sulfate, 1.6-1.7 parts of a bacillus subtilis bacterium agent, 1.2 to 1.4 parts of a protease preparation, 45-47 parts of fresh ageratina adenophora, 20-22 parts of fresh oysters, 1.6-1.9 parts of a pseudomonas aeruginosa bacterium agent, 1.3-1.4 parts of high-temperature cellulose-decomposing bacteria, proper amount of 6mol/L hydrochloric acid solution and proper amount of water. On the basis of supplying nutrients, additional microorganisms and biological enzymes are added into the germination accelerating agent disclosed by the invention and seed coats are properly decomposed by utilizing secretion products generated by movement of the microorganisms, so that the effect of softening the seed coats is realized, and therefore, the permeability of the seed coats is improved and the absorption of nutrients and gas exchange can be facilitated; and the germination of seeds is accelerated and the germination rate and the seedling emergency rate are improved.

Description

A kind of enzymatic presprouting of seeds agent
Technical field
The present invention relates to agricultural Stratification technique field, particularly relate to a kind of enzymatic presprouting of seeds agent.
Background technology
Development along with agricultural, the fast seedling growing of seed becomes the Important Action improving Agriculture, forestry And Animal Husbandry yield of commercial crops, and seed because of external environment be not suitable for or own growth is immature, the seed dormancy phase length that causes, the situation such as germination percentage is low, it is irregular to sprout and germination quality is low, have a strong impact on the growth of later stage crop, and result in production cycle length, the most high series of problems of product quality, affect economic benefit.And germinator can effectively solve the problems referred to above, but current Stratification technique many employings plant growth regulator carries out accelerating germination, not only means are single, simultaneously not for the real needs of all kinds of seeds, environment, kind etc. effective strain be cannot be carried out, the waste of resource and the undesirable of specifically used effect caused.
Eupatorium cannabinum L. (Eupatorium coelestinum L.) is herbaceos perennial, it is that a kind of vitality is strong, breeds the malignant weed being difficult to clean off rapidly, again, it discharges allelochemical by root system secretion, rainfall leaching and Residue decay to soil, suppression other plant growth, form single dominantcommunity, cause the underproduction of agriculture forest and husbandry, endanger huge.And at present utilization to it is focused mostly on and is being sterilized by its allelopathy or controlling plant growing, to the research of its lixiviating solution that becomes thoroughly decomposed with utilize the most relatively fewer.
Polyamines has bioactive small molecule aliphatic non-protein nitrogenous bases compound in being present in higher plant, fungus and prokaryote body, higher at the biological in-vivo content of rich in proteins, polyamines can affect the activity of RNA polymerase in plumular axis, induce seed germination by promoting the synthesis of protein, make it regulate seed germination on nucleic acid and protein level.
Owing to the Eupatorium cannabinum L. (Eupatorium coelestinum L.) lixiviating solution that becomes thoroughly decomposed is rich in available nutrients such as humic acids and nitrogen, phosphorus, potassium, calcium, magnesium and trace element; it is compounded together with the polyamines extracted in Concha Ostreae and forms the preparation with Germination effect; having great development potentiality in agricultural cultivation, the most also the large-scale development for presprouting of seeds nursery industry provides new technical measures.
Summary of the invention
The object of the invention is contemplated to make up the defect of prior art, it is provided that a kind of enzymatic presprouting of seeds agent.
The present invention is achieved by the following technical solutions:
A kind of enzymatic presprouting of seeds agent, is prepared by the raw materials in: Zein powder 26-28, vinegar 9-11, xylitol 2-3, aspirin 0.6-0.8, barium sulfate 0.3-0.4, bacillus subtilis microbial agent 1.6-1.7, protease preparation 1.2-1.4, fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) 45-47, fresh Concha Ostreae 20-22, P. aeruginosa bacteria agent 1.6-1.9, high temperature fiber decomposer microbial inoculum 1.3-1.4,6mol/L hydrochloric acid solution is appropriate, water is appropriate.
Specifically comprising the following steps that of described enzymatic presprouting of seeds agent preparation method
(1) shred after fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) water being rinsed, mix in input compost pond with Zein powder, vinegar, it is separately added into P. aeruginosa bacteria agent and high temperature fiber decomposer microbial inoculum, and keep ferment heap water content at 50-55%, turning add bacillus subtilis microbial agent and protease preparation after fermenting 4-6 days, retting is fermented 15-17 days, air-dries fermentation material after fermentation ends, crossing 1.0-1.2mm sieve after pulverizing, the powder that must become thoroughly decomposed is standby;
(2) Concha Ostreae is shelled take meat and blend, minced meat is mixed by solid-liquid ratio 1:1.5-1.6g/ml with 6mol/L hydrochloric acid solution, after being heated to 55-60 DEG C, insulation is extracted 180-200 minute, it is placed on afterwards in centrifuge, it is centrifuged 28-32 minute under 3000-3100 rev/min, crossing leaching supernatant after Li Xin, regulation PH is to neutral standby;
(3) step 1 gained is become thoroughly decomposed and after powder, xylitol mix with barium sulfate, add the water of 6-8 times of compound weight, it is heated to 65-70 DEG C to react 45-48 minute, is down to room temperature afterwards, add aspirin and stir 8-10 minute, filter with double gauze again, nutritional solution must be chelated standby;
(4) step 2 gained feed liquid, step 3 gained chelating nutritional solution and other leftover materials are sufficiently mixed uniformly, add water and solution is diluted 20-22 times, obtain presprouting of seeds agent of the present invention.
The invention have the advantage that
The present invention is by the compound preparation of polyamines extracted in become thoroughly decomposed Eupatorium cannabinum L. (Eupatorium coelestinum L.) lixiviating solution and Concha Ostreae, detoxification can be reached, stimulation germination and the active matter of growth of seedling can be synthesized again, promote the starch in seed, protein and inositol monophosphate salt hydrolysis, thus contribute to the macro-nutrients hydrolysis in seed endosperm and become little molecular nutrition material necessary to plant structure plant, thus induce the sprouting of seed, and the H that polyamine oxidase degraded produces2O2Promote cell wall ripe and hardening by participating in the effects such as the regulation of cellular redox state, further increase germination percentage and the seedling growth rate of seed.
The germinator of the present invention is on the basis of nutrition supply, by adding extra microorganism and enzyme, and utilize the secretory product of microbial activities that seed coat is decomposed in right amount, reach to soften the effect of seed coat, thus improve kind of a transdermally, be conducive to its absorption to nutrition and exchange of gas, and then accelerate seed germination, improve germination percentage and planting percent.
Detailed description of the invention
A kind of enzymatic presprouting of seeds agent, is made up of the raw material of following weight portion (kg): Zein powder 26, vinegar 9, xylitol 2, aspirin 0.6, barium sulfate 0.3, bacillus subtilis microbial agent 1.6, protease preparation 1.2, fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) 45, fresh Concha Ostreae 20, P. aeruginosa bacteria agent 1.6, high temperature fiber decomposer microbial inoculum 1.3,6mol/L hydrochloric acid solution is appropriate, water is appropriate.
Specifically comprising the following steps that of described enzymatic presprouting of seeds agent preparation method
(1) shred after fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) water being rinsed, mix in input compost pond with Zein powder, vinegar, it is separately added into P. aeruginosa bacteria agent and high temperature fiber decomposer microbial inoculum, and keep ferment heap water content 50%, turning add bacillus subtilis microbial agent and protease preparation after fermenting 4 days, retting is fermented 15 days, air-dries fermentation material after fermentation ends, crossing 1.0mm sieve after pulverizing, the powder that must become thoroughly decomposed is standby;
(2) Concha Ostreae is shelled take meat and blend, minced meat is mixed by solid-liquid ratio 1:1.5g/ml with 6mol/L hydrochloric acid solution, after being heated to 55 DEG C, insulation is extracted 180 minutes, it is placed on afterwards in centrifuge, it is centrifuged 28 minutes under 3000 revs/min, crossing leaching supernatant after Li Xin, regulation PH is to neutral standby;
(3) step 1 gained is become thoroughly decomposed and after powder, xylitol mix with barium sulfate, add the water of 6 times of compound weight, it is heated to 65 DEG C to react 45 minutes, is down to room temperature afterwards, add aspirin and stir 8 minutes, filter with double gauze again, nutritional solution must be chelated standby;
(4) step 2 gained feed liquid, step 3 gained chelating nutritional solution and other leftover materials are sufficiently mixed uniformly, add water and solution is diluted 20 times, obtain presprouting of seeds agent of the present invention.
In order to further illustrate the using value of the present invention, inventor chooses batch of seeds and is equally divided into two groups, one group uses the germinator that common plant growth regulator is made as a control group, another group uses germinator of the present invention as experimental group, two groups of seed culture methods are identical, germinate found that experimental group seed relatively matched group carries the last week, and germination percentage reaches 93%, and matched group only has 78%, the seed germination of experimental group is neat simultaneously, plant bud quality relatively matched group to be obviously improved, it is seen that germinator of the present invention has preferable using effect.

Claims (2)

1. an enzymatic presprouting of seeds agent, it is characterized in that, be prepared by the raw materials in: Zein powder 26-28, vinegar 9-11, xylitol 2-3, aspirin 0.6-0.8, barium sulfate 0.3-0.4, bacillus subtilis microbial agent 1.6-1.7, protease preparation 1.2-1.4, fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) 45-47, fresh Concha Ostreae 20-22, P. aeruginosa bacteria agent 1.6-1.9, high temperature fiber decomposer microbial inoculum 1.3-1.4,6mol/L hydrochloric acid solution is appropriate, water is appropriate.
2. according to the enzymatic presprouting of seeds agent described in claims 1, it is characterised in that specifically comprising the following steps that of preparation method
(1) shred after fresh Eupatorium cannabinum L. (Eupatorium coelestinum L.) water being rinsed, mix in input compost pond with Zein powder, vinegar, it is separately added into P. aeruginosa bacteria agent and high temperature fiber decomposer microbial inoculum, and keep ferment heap water content at 50-55%, turning add bacillus subtilis microbial agent and protease preparation after fermenting 4-6 days, retting is fermented 15-17 days, air-dries fermentation material after fermentation ends, crossing 1.0-1.2mm sieve after pulverizing, the powder that must become thoroughly decomposed is standby;
(2) Concha Ostreae is shelled take meat and blend, minced meat is mixed by solid-liquid ratio 1:1.5-1.6g/ml with 6mol/L hydrochloric acid solution, after being heated to 55-60 DEG C, insulation is extracted 180-200 minute, it is placed on afterwards in centrifuge, it is centrifuged 28-32 minute under 3000-3100 rev/min, crossing leaching supernatant after Li Xin, regulation PH is to neutral standby;
(3) step 1 gained is become thoroughly decomposed and after powder, xylitol mix with barium sulfate, add the water of 6-8 times of compound weight, it is heated to 65-70 DEG C to react 45-48 minute, is down to room temperature afterwards, add aspirin and stir 8-10 minute, filter with double gauze again, nutritional solution must be chelated standby;
(4) step 2 gained feed liquid, step 3 gained chelating nutritional solution and other leftover materials are sufficiently mixed uniformly, add water and solution is diluted 20-22 times, obtain presprouting of seeds agent of the present invention.
CN201610354559.3A 2016-05-26 2016-05-26 Enzyme-catalyzed seed germination accelerating agent Pending CN105901040A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107573165A (en) * 2017-09-30 2018-01-12 广西吉朋投资有限公司 A kind of corn seed germinator
CN108576063A (en) * 2018-05-11 2018-09-28 翟茜子 A kind of biological regulation reagent promoting seed growth

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102795900A (en) * 2012-08-29 2012-11-28 攀枝花市西宇生物科技有限公司 Method for preparing bio-organic fertilizer by utilizing eupatorium adenophorum

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102795900A (en) * 2012-08-29 2012-11-28 攀枝花市西宇生物科技有限公司 Method for preparing bio-organic fertilizer by utilizing eupatorium adenophorum

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
付玉营 等: "多胺对种子萌发生长及成熟的影响", 《种子》 *
郑晓菊 等: "高效液相色谱法测定贝壳类海洋生物中的多胺含量", 《青岛科技大学学报(自然科学版), 》 *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107573165A (en) * 2017-09-30 2018-01-12 广西吉朋投资有限公司 A kind of corn seed germinator
CN108576063A (en) * 2018-05-11 2018-09-28 翟茜子 A kind of biological regulation reagent promoting seed growth

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Application publication date: 20160831