CN105112450A - 抗猪生殖与呼吸综合征病毒的动物 - Google Patents
抗猪生殖与呼吸综合征病毒的动物 Download PDFInfo
- Publication number
- CN105112450A CN105112450A CN201510463471.0A CN201510463471A CN105112450A CN 105112450 A CN105112450 A CN 105112450A CN 201510463471 A CN201510463471 A CN 201510463471A CN 105112450 A CN105112450 A CN 105112450A
- Authority
- CN
- China
- Prior art keywords
- inactivation
- allelotrope
- pig
- gene
- genetic modification
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001135989 Porcine reproductive and respiratory syndrome virus Species 0.000 title abstract description 3
- 241001465754 Metazoa Species 0.000 title description 17
- 238000000034 method Methods 0.000 claims abstract description 131
- 101150087379 CD163 gene Proteins 0.000 claims abstract description 91
- 101150098782 Siglec1 gene Proteins 0.000 claims abstract description 91
- 230000002779 inactivation Effects 0.000 claims description 205
- 238000012239 gene modification Methods 0.000 claims description 148
- 230000005017 genetic modification Effects 0.000 claims description 148
- 235000013617 genetically modified food Nutrition 0.000 claims description 148
- 210000000287 oocyte Anatomy 0.000 claims description 102
- 102100032855 Sialoadhesin Human genes 0.000 claims description 64
- 101000868472 Homo sapiens Sialoadhesin Proteins 0.000 claims description 51
- 210000001161 mammalian embryo Anatomy 0.000 claims description 39
- 230000013011 mating Effects 0.000 claims description 35
- 238000012546 transfer Methods 0.000 claims description 16
- 108700024394 Exon Proteins 0.000 claims description 15
- 230000035935 pregnancy Effects 0.000 claims description 13
- 230000003328 fibroblastic effect Effects 0.000 claims description 12
- 108091005725 scavenger receptor cysteine-rich superfamily Proteins 0.000 claims description 12
- 210000005000 reproductive tract Anatomy 0.000 claims description 10
- 230000012173 estrus Effects 0.000 claims description 9
- 230000004927 fusion Effects 0.000 claims description 9
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 9
- 208000035010 Term birth Diseases 0.000 claims description 7
- 230000016087 ovulation Effects 0.000 claims description 7
- 238000012216 screening Methods 0.000 claims description 7
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims description 5
- 238000002105 Southern blotting Methods 0.000 claims description 5
- 108010078070 scavenger receptors Proteins 0.000 claims description 5
- 102000014452 scavenger receptors Human genes 0.000 claims description 5
- 210000003101 oviduct Anatomy 0.000 claims description 4
- 241001076388 Fimbria Species 0.000 claims description 3
- 230000006798 recombination Effects 0.000 claims description 3
- 238000005215 recombination Methods 0.000 claims description 3
- 238000003752 polymerase chain reaction Methods 0.000 claims 2
- 108090000623 proteins and genes Proteins 0.000 abstract description 64
- 108700028369 Alleles Proteins 0.000 abstract description 6
- 230000009261 transgenic effect Effects 0.000 abstract description 4
- 241000282898 Sus scrofa Species 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 description 60
- 241000700605 Viruses Species 0.000 description 46
- 108010029176 Sialic Acid Binding Ig-like Lectin 1 Proteins 0.000 description 24
- 208000011580 syndromic disease Diseases 0.000 description 23
- 239000003795 chemical substances by application Substances 0.000 description 22
- 108020004414 DNA Proteins 0.000 description 21
- 239000013598 vector Substances 0.000 description 19
- 230000002829 reductive effect Effects 0.000 description 16
- 108091034117 Oligonucleotide Proteins 0.000 description 15
- 230000004913 activation Effects 0.000 description 13
- 230000008859 change Effects 0.000 description 13
- 208000015181 infectious disease Diseases 0.000 description 13
- 230000035772 mutation Effects 0.000 description 12
- 230000008685 targeting Effects 0.000 description 12
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 11
- 241000124008 Mammalia Species 0.000 description 10
- 230000006870 function Effects 0.000 description 10
- 230000004048 modification Effects 0.000 description 10
- 238000012986 modification Methods 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 238000010374 somatic cell nuclear transfer Methods 0.000 description 10
- 241000282887 Suidae Species 0.000 description 9
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 9
- 210000002950 fibroblast Anatomy 0.000 description 9
- 230000006801 homologous recombination Effects 0.000 description 9
- 238000002744 homologous recombination Methods 0.000 description 9
- 239000000047 product Substances 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- SQVRNKJHWKZAKO-OQPLDHBCSA-N sialic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)OC1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-OQPLDHBCSA-N 0.000 description 9
- 230000000694 effects Effects 0.000 description 8
- 235000018102 proteins Nutrition 0.000 description 8
- 230000002441 reversible effect Effects 0.000 description 8
- 230000015572 biosynthetic process Effects 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 230000003612 virological effect Effects 0.000 description 7
- 150000001413 amino acids Chemical class 0.000 description 6
- 238000004520 electroporation Methods 0.000 description 6
- PGBHMTALBVVCIT-VCIWKGPPSA-N framycetin Chemical compound N[C@@H]1[C@@H](O)[C@H](O)[C@H](CN)O[C@@H]1O[C@H]1[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](N)C[C@@H](N)[C@@H]2O)O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CN)O2)N)O[C@@H]1CO PGBHMTALBVVCIT-VCIWKGPPSA-N 0.000 description 6
- 230000006698 induction Effects 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 239000013612 plasmid Substances 0.000 description 6
- 108020003175 receptors Proteins 0.000 description 6
- 102000005962 receptors Human genes 0.000 description 6
- 229960005486 vaccine Drugs 0.000 description 6
- 208000037581 Persistent Infection Diseases 0.000 description 5
- 101000868473 Sus scrofa Sialoadhesin Proteins 0.000 description 5
- 239000002253 acid Substances 0.000 description 5
- 235000001014 amino acid Nutrition 0.000 description 5
- 230000027455 binding Effects 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 5
- 238000010367 cloning Methods 0.000 description 5
- 210000005220 cytoplasmic tail Anatomy 0.000 description 5
- 238000013461 design Methods 0.000 description 5
- 230000007159 enucleation Effects 0.000 description 5
- 230000001605 fetal effect Effects 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 239000002773 nucleotide Substances 0.000 description 5
- 125000003729 nucleotide group Chemical group 0.000 description 5
- 238000012797 qualification Methods 0.000 description 5
- 238000001890 transfection Methods 0.000 description 5
- 108010051219 Cre recombinase Proteins 0.000 description 4
- 241001071864 Lethrinus laticaudis Species 0.000 description 4
- 208000005342 Porcine Reproductive and Respiratory Syndrome Diseases 0.000 description 4
- 230000001154 acute effect Effects 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 210000004940 nucleus Anatomy 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- UMGDCJDMYOKAJW-UHFFFAOYSA-N thiourea Chemical compound NC(N)=S UMGDCJDMYOKAJW-UHFFFAOYSA-N 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- 238000011282 treatment Methods 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- VWAUPFMBXBWEQY-ANULTFPQSA-N Altrenogest Chemical compound C1CC(=O)C=C2CC[C@@H]([C@H]3[C@@](C)([C@](CC3)(O)CC=C)C=C3)C3=C21 VWAUPFMBXBWEQY-ANULTFPQSA-N 0.000 description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 101710163270 Nuclease Proteins 0.000 description 3
- 102000007073 Sialic Acid Binding Immunoglobulin-like Lectins Human genes 0.000 description 3
- 108010047827 Sialic Acid Binding Immunoglobulin-like Lectins Proteins 0.000 description 3
- 210000001132 alveolar macrophage Anatomy 0.000 description 3
- 238000000137 annealing Methods 0.000 description 3
- 230000000692 anti-sense effect Effects 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 230000035606 childbirth Effects 0.000 description 3
- 238000012217 deletion Methods 0.000 description 3
- 230000037430 deletion Effects 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000010363 gene targeting Methods 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000003607 modifier Substances 0.000 description 3
- 210000004681 ovum Anatomy 0.000 description 3
- 210000004508 polar body Anatomy 0.000 description 3
- 230000002685 pulmonary effect Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 238000010361 transduction Methods 0.000 description 3
- 230000026683 transduction Effects 0.000 description 3
- 210000004340 zona pellucida Anatomy 0.000 description 3
- 241000710929 Alphavirus Species 0.000 description 2
- 241001292006 Arteriviridae Species 0.000 description 2
- 108010077544 Chromatin Proteins 0.000 description 2
- 241000710803 Equine arteritis virus Species 0.000 description 2
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 2
- 108060003951 Immunoglobulin Proteins 0.000 description 2
- LSDPWZHWYPCBBB-UHFFFAOYSA-N Methanethiol Chemical compound SC LSDPWZHWYPCBBB-UHFFFAOYSA-N 0.000 description 2
- 101000868474 Mus musculus Sialoadhesin Proteins 0.000 description 2
- 108020004485 Nonsense Codon Proteins 0.000 description 2
- 101150001779 ORF1a gene Proteins 0.000 description 2
- 241000501499 Sialis Species 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 239000005864 Sulphur Substances 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- 102000006601 Thymidine Kinase Human genes 0.000 description 2
- 108020004440 Thymidine kinase Proteins 0.000 description 2
- 108010017070 Zinc Finger Nucleases Proteins 0.000 description 2
- 230000003187 abdominal effect Effects 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 210000002459 blastocyst Anatomy 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 239000001506 calcium phosphate Substances 0.000 description 2
- 229910000389 calcium phosphate Inorganic materials 0.000 description 2
- 235000011010 calcium phosphates Nutrition 0.000 description 2
- 210000003483 chromatin Anatomy 0.000 description 2
- 230000000295 complement effect Effects 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 210000001771 cumulus cell Anatomy 0.000 description 2
- 230000024835 cytogamy Effects 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 238000009792 diffusion process Methods 0.000 description 2
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 description 2
- 230000005611 electricity Effects 0.000 description 2
- 230000012202 endocytosis Effects 0.000 description 2
- 150000002148 esters Chemical class 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 238000013467 fragmentation Methods 0.000 description 2
- 238000006062 fragmentation reaction Methods 0.000 description 2
- 238000003209 gene knockout Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 102000018358 immunoglobulin Human genes 0.000 description 2
- 230000002458 infectious effect Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 210000002540 macrophage Anatomy 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 230000037434 nonsense mutation Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000008506 pathogenesis Effects 0.000 description 2
- 230000010363 phase shift Effects 0.000 description 2
- 239000002953 phosphate buffered saline Substances 0.000 description 2
- -1 polyoxyethylene Polymers 0.000 description 2
- 239000002243 precursor Substances 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 230000001681 protective effect Effects 0.000 description 2
- 229940050570 regu-mate Drugs 0.000 description 2
- 238000012552 review Methods 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 230000001568 sexual effect Effects 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 230000001502 supplementing effect Effects 0.000 description 2
- CIHOLLKRGTVIJN-UHFFFAOYSA-N tert‐butyl hydroperoxide Chemical compound CC(C)(C)OO CIHOLLKRGTVIJN-UHFFFAOYSA-N 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 238000013518 transcription Methods 0.000 description 2
- 230000035897 transcription Effects 0.000 description 2
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
- 241000701161 unidentified adenovirus Species 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 230000009385 viral infection Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- OZFAFGSSMRRTDW-UHFFFAOYSA-N (2,4-dichlorophenyl) benzenesulfonate Chemical compound ClC1=CC(Cl)=CC=C1OS(=O)(=O)C1=CC=CC=C1 OZFAFGSSMRRTDW-UHFFFAOYSA-N 0.000 description 1
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 1
- SLAMLWHELXOEJZ-UHFFFAOYSA-N 2-nitrobenzoic acid Chemical compound OC(=O)C1=CC=CC=C1[N+]([O-])=O SLAMLWHELXOEJZ-UHFFFAOYSA-N 0.000 description 1
- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 description 1
- RZVAJINKPMORJF-UHFFFAOYSA-N Acetaminophen Chemical compound CC(=O)NC1=CC=C(O)C=C1 RZVAJINKPMORJF-UHFFFAOYSA-N 0.000 description 1
- 101000621943 Acholeplasma phage L2 Probable integrase/recombinase Proteins 0.000 description 1
- 241001516864 Allende Species 0.000 description 1
- 101000618348 Allochromatium vinosum (strain ATCC 17899 / DSM 180 / NBRC 103801 / NCIMB 10441 / D) Uncharacterized protein Alvin_0065 Proteins 0.000 description 1
- 108090000328 Arrestin Proteins 0.000 description 1
- 102000003916 Arrestin Human genes 0.000 description 1
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 1
- 101000781117 Autographa californica nuclear polyhedrosis virus Uncharacterized 12.4 kDa protein in CTL-LEF2 intergenic region Proteins 0.000 description 1
- 101000708323 Azospirillum brasilense Uncharacterized 28.8 kDa protein in nifR3-like 5'region Proteins 0.000 description 1
- 101000770311 Azotobacter chroococcum mcd 1 Uncharacterized 19.8 kDa protein in nifW 5'region Proteins 0.000 description 1
- 101000748761 Bacillus subtilis (strain 168) Uncharacterized MFS-type transporter YcxA Proteins 0.000 description 1
- 101000765620 Bacillus subtilis (strain 168) Uncharacterized protein YlxP Proteins 0.000 description 1
- 101000916134 Bacillus subtilis (strain 168) Uncharacterized protein YqxJ Proteins 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 101000754349 Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251) UPF0065 protein BP0148 Proteins 0.000 description 1
- 101000827633 Caldicellulosiruptor sp. (strain Rt8B.4) Uncharacterized 23.9 kDa protein in xynA 3'region Proteins 0.000 description 1
- 102000005853 Clathrin Human genes 0.000 description 1
- 108010019874 Clathrin Proteins 0.000 description 1
- 101000947628 Claviceps purpurea Uncharacterized 11.8 kDa protein Proteins 0.000 description 1
- 101000686796 Clostridium perfringens Replication protein Proteins 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 1
- 101710088194 Dehydrogenase Proteins 0.000 description 1
- 239000012591 Dulbecco’s Phosphate Buffered Saline Substances 0.000 description 1
- 101710121417 Envelope glycoprotein Proteins 0.000 description 1
- 101000788129 Escherichia coli Uncharacterized protein in sul1 3'region Proteins 0.000 description 1
- 101000788370 Escherichia phage P2 Uncharacterized 12.9 kDa protein in GpA 3'region Proteins 0.000 description 1
- 102000003974 Fibroblast growth factor 2 Human genes 0.000 description 1
- 108090000379 Fibroblast growth factor 2 Proteins 0.000 description 1
- 101150034914 GP2a gene Proteins 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- 101000787096 Geobacillus stearothermophilus Uncharacterized protein in gldA 3'region Proteins 0.000 description 1
- 102100038353 Gremlin-2 Human genes 0.000 description 1
- 101000976889 Haemophilus phage HP1 (strain HP1c1) Uncharacterized 19.2 kDa protein in cox-rep intergenic region Proteins 0.000 description 1
- 206010061192 Haemorrhagic fever Diseases 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 208000005331 Hepatitis D Diseases 0.000 description 1
- 229920000209 Hexadimethrine bromide Polymers 0.000 description 1
- 102000016844 Immunoglobulin-like domains Human genes 0.000 description 1
- 108050006430 Immunoglobulin-like domains Proteins 0.000 description 1
- 208000029523 Interstitial Lung disease Diseases 0.000 description 1
- 240000007049 Juglans regia Species 0.000 description 1
- 101000827627 Klebsiella pneumoniae Putative low molecular weight protein-tyrosine-phosphatase Proteins 0.000 description 1
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 description 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- 101710197072 Lectin 1 Proteins 0.000 description 1
- 229930195725 Mannitol Natural products 0.000 description 1
- 101001130841 Middle East respiratory syndrome-related coronavirus (isolate United Kingdom/H123990006/2012) Non-structural protein ORF5 Proteins 0.000 description 1
- 101001032860 Mus musculus Gremlin-2 Proteins 0.000 description 1
- SPEFJYZGXZENAF-UHFFFAOYSA-N N-cyclohexyl-1-(2,4-dichlorophenyl)-6-methyl-4H-indeno[1,2-c]pyrazole-3-carboxamide Chemical compound C=1C(C)=CC=C(C2=3)C=1CC=3C(C(=O)NC1CCCCC1)=NN2C1=CC=C(Cl)C=C1Cl SPEFJYZGXZENAF-UHFFFAOYSA-N 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 239000012124 Opti-MEM Substances 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 108010076039 Polyproteins Proteins 0.000 description 1
- 206010067268 Post procedural infection Diseases 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 102000001253 Protein Kinase Human genes 0.000 description 1
- 208000018569 Respiratory Tract disease Diseases 0.000 description 1
- 206010038997 Retroviral infections Diseases 0.000 description 1
- 101000974028 Rhizobium leguminosarum bv. viciae (strain 3841) Putative cystathionine beta-lyase Proteins 0.000 description 1
- 101000756519 Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003) Uncharacterized protein RCAP_rcc00048 Proteins 0.000 description 1
- 101000948219 Rhodococcus erythropolis Uncharacterized 11.5 kDa protein in thcD 3'region Proteins 0.000 description 1
- 101150065831 SIGLEC13 gene Proteins 0.000 description 1
- 102000000185 SRCR domains Human genes 0.000 description 1
- 108050008568 SRCR domains Proteins 0.000 description 1
- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 241001516645 Simian hemorrhagic fever virus Species 0.000 description 1
- 108020004459 Small interfering RNA Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 101000936711 Streptococcus gordonii Accessory secretory protein Asp4 Proteins 0.000 description 1
- 101000929863 Streptomyces cinnamonensis Monensin polyketide synthase putative ketoacyl reductase Proteins 0.000 description 1
- 101000788468 Streptomyces coelicolor Uncharacterized protein in mprR 3'region Proteins 0.000 description 1
- 101000845085 Streptomyces violaceoruber Granaticin polyketide synthase putative ketoacyl reductase 1 Proteins 0.000 description 1
- 102100021696 Syncytin-1 Human genes 0.000 description 1
- 206010051379 Systemic Inflammatory Response Syndrome Diseases 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 108010014401 TWEAK Receptor Proteins 0.000 description 1
- 101000711771 Thiocystis violacea Uncharacterized 76.5 kDa protein in phbC 3'region Proteins 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 102100028786 Tumor necrosis factor receptor superfamily member 12A Human genes 0.000 description 1
- 101000711318 Vibrio alginolyticus Uncharacterized 11.6 kDa protein in scrR 3'region Proteins 0.000 description 1
- 206010058874 Viraemia Diseases 0.000 description 1
- 108010066342 Virus Receptors Proteins 0.000 description 1
- 102000018265 Virus Receptors Human genes 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 229960000971 altrenogest Drugs 0.000 description 1
- 210000002821 alveolar epithelial cell Anatomy 0.000 description 1
- 125000000539 amino acid group Chemical group 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 230000006907 apoptotic process Effects 0.000 description 1
- 206010003230 arteritis Diseases 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 238000002869 basic local alignment search tool Methods 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- AINRUFPWSLJTCA-UHFFFAOYSA-N benzoic acid;mercury Chemical compound C1=CC(C(=O)O)=C[C@H]2[Hg]C21 AINRUFPWSLJTCA-UHFFFAOYSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- FOCUKKUAGZPPCU-UHFFFAOYSA-N boxidine Chemical compound C1=CC(C(F)(F)F)=CC=C1C(C=C1)=CC=C1OCCN1CCCC1 FOCUKKUAGZPPCU-UHFFFAOYSA-N 0.000 description 1
- 229950010104 boxidine Drugs 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- KDPAWGWELVVRCH-UHFFFAOYSA-M bromoacetate Chemical compound [O-]C(=O)CBr KDPAWGWELVVRCH-UHFFFAOYSA-M 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 229910002091 carbon monoxide Inorganic materials 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003638 chemical reducing agent Substances 0.000 description 1
- FOCAUTSVDIKZOP-UHFFFAOYSA-N chloroacetic acid Chemical compound OC(=O)CCl FOCAUTSVDIKZOP-UHFFFAOYSA-N 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 229930193282 clathrin Natural products 0.000 description 1
- 238000011284 combination treatment Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000014507 cortical granule exocytosis Effects 0.000 description 1
- JVHIPYJQMFNCEK-UHFFFAOYSA-N cytochalasin Natural products N1C(=O)C2(C(C=CC(C)CC(C)CC=C3)OC(C)=O)C3C(O)C(=C)C(C)C2C1CC1=CC=CC=C1 JVHIPYJQMFNCEK-UHFFFAOYSA-N 0.000 description 1
- ZMAODHOXRBLOQO-UHFFFAOYSA-N cytochalasin-A Natural products N1C(=O)C23OC(=O)C=CC(=O)CCCC(C)CC=CC3C(O)C(=C)C(C)C2C1CC1=CC=CC=C1 ZMAODHOXRBLOQO-UHFFFAOYSA-N 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 241001493065 dsRNA viruses Species 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 210000001671 embryonic stem cell Anatomy 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 206010014599 encephalitis Diseases 0.000 description 1
- 210000001163 endosome Anatomy 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- CEAZRRDELHUEMR-UHFFFAOYSA-N gentamicin Chemical class O1C(C(C)NC)CCC(N)C1OC1C(O)C(OC2C(C(NC)C(C)(O)CO2)O)C(N)CC1N CEAZRRDELHUEMR-UHFFFAOYSA-N 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 1
- 229960003180 glutathione Drugs 0.000 description 1
- 235000003969 glutathione Nutrition 0.000 description 1
- YPZRWBKMTBYPTK-BJDJZHNGSA-N glutathione disulfide Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@H](C(=O)NCC(O)=O)CSSC[C@@H](C(=O)NCC(O)=O)NC(=O)CC[C@H](N)C(O)=O YPZRWBKMTBYPTK-BJDJZHNGSA-N 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000002955 immunomodulating agent Substances 0.000 description 1
- 230000002584 immunomodulator Effects 0.000 description 1
- 229940121354 immunomodulator Drugs 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 230000009027 insemination Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000012212 insulator Substances 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- JDNTWHVOXJZDSN-UHFFFAOYSA-N iodoacetic acid Chemical compound OC(=O)CI JDNTWHVOXJZDSN-UHFFFAOYSA-N 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 238000001638 lipofection Methods 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 230000000527 lymphocytic effect Effects 0.000 description 1
- 210000003563 lymphoid tissue Anatomy 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 239000000594 mannitol Substances 0.000 description 1
- 235000010355 mannitol Nutrition 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000002611 ovarian Effects 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 108010064607 phosphoglucokinase Proteins 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 108060006633 protein kinase Proteins 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000010837 receptor-mediated endocytosis Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 208000023504 respiratory system disease Diseases 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 210000000582 semen Anatomy 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 108010066925 sleep-promoting factor B Proteins 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- NZSYKLNULKPWIX-UHFFFAOYSA-M sodium;2-methylsulfonylsulfanylethanesulfonate Chemical compound [Na+].CS(=O)(=O)SCCS([O-])(=O)=O NZSYKLNULKPWIX-UHFFFAOYSA-M 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000009871 tenuigenin Substances 0.000 description 1
- 210000001550 testis Anatomy 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- CWERGRDVMFNCDR-UHFFFAOYSA-M thioglycolate(1-) Chemical class [O-]C(=O)CS CWERGRDVMFNCDR-UHFFFAOYSA-M 0.000 description 1
- 125000003396 thiol group Chemical group [H]S* 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 102000027257 transmembrane receptors Human genes 0.000 description 1
- 108091008578 transmembrane receptors Proteins 0.000 description 1
- DZWJBKUVHJSHAR-UHFFFAOYSA-M trimethyl(2-methylsulfonylsulfanylethyl)azanium;bromide Chemical compound [Br-].C[N+](C)(C)CCSS(C)(=O)=O DZWJBKUVHJSHAR-UHFFFAOYSA-M 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 241001430294 unidentified retrovirus Species 0.000 description 1
- 244000052613 viral pathogen Species 0.000 description 1
- 210000002845 virion Anatomy 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/108—Swine
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/7056—Lectin superfamily, e.g. CD23, CD72
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
- C07K14/70596—Molecules with a "CD"-designation not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Animal Behavior & Ethology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161519076P | 2011-05-16 | 2011-05-16 | |
| US61/519,076 | 2011-05-16 | ||
| CN201280023487.4A CN103687482A (zh) | 2011-05-16 | 2012-05-16 | 抗猪生殖与呼吸综合征病毒的动物 |
Related Parent Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280023487.4A Division CN103687482A (zh) | 2011-05-16 | 2012-05-16 | 抗猪生殖与呼吸综合征病毒的动物 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105112450A true CN105112450A (zh) | 2015-12-02 |
Family
ID=47177326
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510463471.0A Pending CN105112450A (zh) | 2011-05-16 | 2012-05-16 | 抗猪生殖与呼吸综合征病毒的动物 |
| CN201280023487.4A Pending CN103687482A (zh) | 2011-05-16 | 2012-05-16 | 抗猪生殖与呼吸综合征病毒的动物 |
| CN202010588447.0A Pending CN111593025A (zh) | 2011-05-16 | 2012-05-16 | 抗猪生殖与呼吸综合征病毒的动物 |
Family Applications After (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201280023487.4A Pending CN103687482A (zh) | 2011-05-16 | 2012-05-16 | 抗猪生殖与呼吸综合征病毒的动物 |
| CN202010588447.0A Pending CN111593025A (zh) | 2011-05-16 | 2012-05-16 | 抗猪生殖与呼吸综合征病毒的动物 |
Country Status (21)
| Country | Link |
|---|---|
| US (5) | US9820475B2 (ru) |
| EP (2) | EP3420813A1 (ru) |
| JP (5) | JP2014519323A (ru) |
| KR (5) | KR102428306B1 (ru) |
| CN (3) | CN105112450A (ru) |
| AU (5) | AU2012255744A1 (ru) |
| BR (1) | BR112013029498A8 (ru) |
| CA (2) | CA3184311A1 (ru) |
| CY (1) | CY1120890T1 (ru) |
| DK (1) | DK2709445T3 (ru) |
| ES (1) | ES2691618T3 (ru) |
| HK (1) | HK1215282A1 (ru) |
| HR (1) | HRP20181729T1 (ru) |
| HU (1) | HUE039793T2 (ru) |
| LT (1) | LT2709445T (ru) |
| MX (1) | MX357421B (ru) |
| PL (1) | PL2709445T3 (ru) |
| PT (1) | PT2709445T (ru) |
| RU (1) | RU2644673C2 (ru) |
| SI (1) | SI2709445T1 (ru) |
| WO (1) | WO2012158828A1 (ru) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109475107A (zh) * | 2015-08-06 | 2019-03-15 | 密苏里大学管理者 | 具有修饰的cd163基因的抗病原体动物 |
| CN112313330A (zh) * | 2018-04-27 | 2021-02-02 | 密苏里大学管理者 | 具有经修饰氨肽酶n(anpep)基因的抗病原体动物 |
| CN113151291A (zh) * | 2020-05-05 | 2021-07-23 | 吉纳斯公司 | 通过对cd163靶向灭活来改善猪类健康的方法 |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3184311A1 (en) * | 2011-05-16 | 2012-11-22 | The Curators Of The University Of Missouri | Porcine reproductive and respiratory syndrome virus resistant animals |
| GB201313235D0 (en) * | 2013-07-24 | 2013-09-04 | Univ Edinburgh | Antiviral Compositions Methods and Animals |
| US20150275231A1 (en) * | 2014-03-31 | 2015-10-01 | Mice With Horns, Llc | Method of Preventing or Reducing Virus Transmission in Animals |
| CN104593422A (zh) * | 2015-01-08 | 2015-05-06 | 中国农业大学 | 一种抗蓝耳病克隆猪的制备方法 |
| JP6929527B2 (ja) * | 2015-03-04 | 2021-09-01 | 株式会社ポル・メド・テック | 安定した表現型を示す疾患モデルブタおよびその作製方法 |
| KR102631856B1 (ko) * | 2015-08-06 | 2024-02-01 | 더 큐레이터스 오브 더 유니버시티 오브 미주리 | 변형된 cd163 유전자를 갖는 병원균-내성 동물 |
| GB201617559D0 (en) | 2016-10-17 | 2016-11-30 | University Court Of The University Of Edinburgh The | Swine comprising modified cd163 and associated methods |
| CN107937345B (zh) * | 2017-11-16 | 2019-01-29 | 山东蓝思种业股份有限公司 | 一种制备同时敲除cd163基因和cd13基因的猪成纤维细胞的方法 |
| CA3096022A1 (en) * | 2018-04-17 | 2019-10-24 | The Curators Of The University Of Missouri | Methods for protecting porcine fetuses from infection with virus |
| US11160260B2 (en) | 2018-04-17 | 2021-11-02 | The Curators Of The University Of Missouri | Methods for protecting porcine fetuses from infection with porcine reproductive and respiratory syndrome virus (PRRSV) |
| CN109197780A (zh) * | 2018-11-26 | 2019-01-15 | 湖北天牧畜禽有限公司 | 一种减少仔猪生病的养殖方法 |
| AU2020325750A1 (en) * | 2019-08-06 | 2022-02-17 | Nzeno Limited | Donor pigs for xenotransplantation |
| EP4276109A1 (en) * | 2021-01-05 | 2023-11-15 | Public University Corporation Yokohama City University | Biomarker for determining fertility, and determining method using same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050260176A1 (en) * | 2002-08-21 | 2005-11-24 | Revivicor, Inc. | Tissue products derived from animals lacking any expression of functional alpha 1,3 galactosyltransferase |
| US20100158947A1 (en) * | 2007-07-27 | 2010-06-24 | Ghent University | Permissive cells and uses thereof |
| US20110038841A1 (en) * | 2009-08-14 | 2011-02-17 | David Ayares | Multi-Transgenic Pigs for Diabetes Treatment |
Family Cites Families (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1995016670A1 (en) | 1993-12-16 | 1995-06-22 | The Procter & Gamble Company | Process for making sulfonated fatty acid alkyl ester surfactant |
| GB9417831D0 (en) | 1994-09-05 | 1994-10-26 | Biotech & Biolog Scien Res | Biological manipulation |
| US5683993A (en) | 1995-06-22 | 1997-11-04 | Ciba Vision Corporation | Compositions and methods for stabilizing polymers |
| JP4497559B2 (ja) | 1995-06-22 | 2010-07-07 | スリーエム カンパニー | 安定なヒドロアルコール組成物 |
| GB9517779D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| GB9517780D0 (en) | 1995-08-31 | 1995-11-01 | Roslin Inst Edinburgh | Biological manipulation |
| JPH09298981A (ja) * | 1996-05-16 | 1997-11-25 | Y S New Technol Kenkyusho:Kk | トランスジェニックブタ |
| US5945577A (en) | 1997-01-10 | 1999-08-31 | University Of Massachusetts As Represented By Its Amherst Campus | Cloning using donor nuclei from proliferating somatic cells |
| US5995577A (en) | 1997-02-10 | 1999-11-30 | General Electric Company | Optimized steam vent locations for a nuclear fuel bundle |
| AU7979098A (en) | 1997-06-18 | 1999-01-04 | Curators Of The University Of Missouri, The | Complete activation of mammalian oocytes |
| US6740490B2 (en) * | 2001-01-29 | 2004-05-25 | Kansas State University Research Foundation | Identification and applications of porcine reproductive and respiratory syndrome virus host susceptibility factor(s) for improved swine breeding |
| EP1465481A4 (en) | 2001-12-21 | 2007-09-12 | Univ Missouri | KNOCK-OUT PORK AND METHODS OF MAKING THE SAME |
| WO2005104835A2 (en) | 2004-04-22 | 2005-11-10 | Kirin Beer Kabushiki Kaisha | Transgenic animals and uses thereof |
| CN101678116A (zh) | 2006-05-11 | 2010-03-24 | 根特大学 | 通过唾液酸粘附素靶向巨噬细胞 |
| US8563313B2 (en) * | 2007-02-13 | 2013-10-22 | Washington State University Research Foundation | Macrophage cell-lines for propagation of porcine reproductive and respiratory syndrome virus |
| KR20100021561A (ko) * | 2007-03-07 | 2010-02-25 | 오르후스 우니베르시테트 | 건선 돼지 모델 |
| US8912386B2 (en) | 2007-03-28 | 2014-12-16 | University Of Iowa Research Foundation | Transgenic pig model of cystic fibrosis |
| US20110016543A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Genomic editing of genes involved in inflammation |
| US20110016546A1 (en) * | 2008-12-04 | 2011-01-20 | Sigma-Aldrich Co. | Porcine genome editing with zinc finger nucleases |
| CA3184311A1 (en) * | 2011-05-16 | 2012-11-22 | The Curators Of The University Of Missouri | Porcine reproductive and respiratory syndrome virus resistant animals |
| EP3331355B1 (en) | 2015-08-06 | 2024-04-03 | The Curators of the University of Missouri | Porcine reproductive and respiratory syndrome virus (prrsv)-resistant porcine and cells having modified cd163 genes |
-
2012
- 2012-05-16 CA CA3184311A patent/CA3184311A1/en active Pending
- 2012-05-16 KR KR1020207015089A patent/KR102428306B1/ko active IP Right Grant
- 2012-05-16 EP EP18189039.3A patent/EP3420813A1/en active Pending
- 2012-05-16 CN CN201510463471.0A patent/CN105112450A/zh active Pending
- 2012-05-16 RU RU2013155677A patent/RU2644673C2/ru active
- 2012-05-16 LT LTEP12785983.3T patent/LT2709445T/lt unknown
- 2012-05-16 KR KR1020247001725A patent/KR20240014587A/ko not_active Application Discontinuation
- 2012-05-16 AU AU2012255744A patent/AU2012255744A1/en not_active Abandoned
- 2012-05-16 WO PCT/US2012/038193 patent/WO2012158828A1/en active Application Filing
- 2012-05-16 HU HUE12785983A patent/HUE039793T2/hu unknown
- 2012-05-16 PL PL12785983T patent/PL2709445T3/pl unknown
- 2012-05-16 CN CN201280023487.4A patent/CN103687482A/zh active Pending
- 2012-05-16 MX MX2013013427A patent/MX357421B/es active IP Right Grant
- 2012-05-16 JP JP2014511498A patent/JP2014519323A/ja active Pending
- 2012-05-16 KR KR1020227026347A patent/KR102574897B1/ko active IP Right Grant
- 2012-05-16 US US14/117,943 patent/US9820475B2/en active Active
- 2012-05-16 ES ES12785983.3T patent/ES2691618T3/es active Active
- 2012-05-16 SI SI201231423T patent/SI2709445T1/sl unknown
- 2012-05-16 BR BR112013029498A patent/BR112013029498A8/pt not_active Application Discontinuation
- 2012-05-16 EP EP12785983.3A patent/EP2709445B1/en active Active
- 2012-05-16 PT PT12785983T patent/PT2709445T/pt unknown
- 2012-05-16 CA CA2836288A patent/CA2836288A1/en active Pending
- 2012-05-16 CN CN202010588447.0A patent/CN111593025A/zh active Pending
- 2012-05-16 KR KR1020237029748A patent/KR102627319B1/ko active IP Right Grant
- 2012-05-16 KR KR1020137033359A patent/KR20140034229A/ko not_active Application Discontinuation
- 2012-05-16 DK DK12785983.3T patent/DK2709445T3/en active
-
2016
- 2016-03-21 HK HK16103259.2A patent/HK1215282A1/zh unknown
-
2017
- 2017-04-19 AU AU2017202540A patent/AU2017202540B2/en active Active
- 2017-05-09 JP JP2017092768A patent/JP6970525B2/ja active Active
- 2017-10-13 US US15/783,417 patent/US10080353B2/en active Active
-
2018
- 2018-08-24 US US16/111,631 patent/US10405526B2/en active Active
- 2018-10-22 HR HRP20181729TT patent/HRP20181729T1/hr unknown
- 2018-11-02 AU AU2018256670A patent/AU2018256670B2/en active Active
- 2018-11-07 CY CY181101169T patent/CY1120890T1/el unknown
-
2019
- 2019-07-04 JP JP2019125475A patent/JP2019193660A/ja not_active Withdrawn
- 2019-08-30 US US16/556,878 patent/US11019809B2/en active Active
-
2020
- 2020-12-24 AU AU2020294305A patent/AU2020294305A1/en not_active Abandoned
-
2021
- 2021-04-28 US US17/242,963 patent/US20210259219A1/en active Pending
-
2022
- 2022-03-16 JP JP2022041628A patent/JP2022081637A/ja not_active Withdrawn
-
2023
- 2023-08-22 AU AU2023219858A patent/AU2023219858A1/en active Pending
-
2024
- 2024-03-06 JP JP2024033472A patent/JP2024069309A/ja active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050260176A1 (en) * | 2002-08-21 | 2005-11-24 | Revivicor, Inc. | Tissue products derived from animals lacking any expression of functional alpha 1,3 galactosyltransferase |
| US20100158947A1 (en) * | 2007-07-27 | 2010-06-24 | Ghent University | Permissive cells and uses thereof |
| US20110038841A1 (en) * | 2009-08-14 | 2011-02-17 | David Ayares | Multi-Transgenic Pigs for Diabetes Treatment |
Non-Patent Citations (2)
| Title |
|---|
| 孙兴参 等: "猪卵母细胞孤雌激活方法及影响因素的研究", 《中国农业科学》 * |
| 王凤雪等: "猪繁殖与呼吸综合征病毒感染相关受体的研究概况", 《黑龙江畜牧兽医》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109475107A (zh) * | 2015-08-06 | 2019-03-15 | 密苏里大学管理者 | 具有修饰的cd163基因的抗病原体动物 |
| CN112313330A (zh) * | 2018-04-27 | 2021-02-02 | 密苏里大学管理者 | 具有经修饰氨肽酶n(anpep)基因的抗病原体动物 |
| CN113151291A (zh) * | 2020-05-05 | 2021-07-23 | 吉纳斯公司 | 通过对cd163靶向灭活来改善猪类健康的方法 |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105112450A (zh) | 抗猪生殖与呼吸综合征病毒的动物 | |
| US20240122164A1 (en) | Pathogen-resistant animals having modified cd163 genes | |
| US20200236914A1 (en) | Pathogen-resistant animals having modified aminopeptidase n (anpep) genes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1215282 Country of ref document: HK |
|
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151202 |
|
| RJ01 | Rejection of invention patent application after publication |