CN105110827A - Organic fertilizer prepared from Salvinia nutans and leaf of Ficus microcarpa - Google Patents
Organic fertilizer prepared from Salvinia nutans and leaf of Ficus microcarpa Download PDFInfo
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- CN105110827A CN105110827A CN201510447597.9A CN201510447597A CN105110827A CN 105110827 A CN105110827 A CN 105110827A CN 201510447597 A CN201510447597 A CN 201510447597A CN 105110827 A CN105110827 A CN 105110827A
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Abstract
The invention relates to organic fertilizer prepared from Salvinia nutans and leaf of Ficus microcarpa. The organic fertilizer is a novel organic fertilizer with excellent fertility. The organic fertilizer is prepared from base raw materials of Salvinia nutans and leaf of Ficus microcarpa according to a mass ratio of 2: 1 by a solid fermentation method. The organic fertilizer can substantially improve a crop yield and can prevent and treat sclerotinia rot of colza.
Description
Technical field
The present invention relates to fertilizer field, especially organic fertilizer technical field.
Background technology
Organic substance in organic fertilizer and nutritive substance are all very abundant, can not only provide required nutrient, can also improve crop yield, improve crop quality, preservation of fertility for the growth of crop, can improve utilization rate of fertilizer simultaneously, reduce production cost.
Along with the raising of people's living standard, the demand of free of contamination organic, the green food of safety and sanitation is in continuous increase, and vast farmers improves the market competitiveness of agricultural-food in the urgent need to application of organic fertilizers.At present, western countries' organic fertilizer consumptions such as the U.S. have accounted for 50% of total amount, and China's Organic fertilizer industry merchandized handling begins to take shape, and using on a large scale of farmland is not yet universal, and institute's accounting is less than 10%.
Rape is the main oil crops of China, and sclerotinia rot of colza is one of important disease in Rape-seed production, and long-term strain sickness rate is up to 10% ~ 30%, and serious reaches more than 80%; The general underproduction 10% ~ 70% of diseased plant.
The Pathogenic Strains sclerotinite (Sclerotiniasclerotiorum (Lib.) deBary) of sclerotinia rot of colza, belongs to Ascomycotina fungi.Sclerotium Long Circle, to irregular shape, like mouse excrement shape, grays after first white, inner canescence.Grow 1 after sclerotial germination to the meat tawny plate-like apothecium of multiple tool long handle, on dish raw one deck ascus and lateral filament, the colourless club-like of ascus, include the colourless thecaspore of unit cell 8, lateral filament is colourless, thread, half-cooked between ascus.
Natant Saluinia Herb is the herb of Salviniaceae plant Chinese scholartree leaf duckweed Salvinianutans.There is relieving superficies by cooling, inducing diuresis to remove edema, effect of removing toxic substances.
Leaf of Smallfruit Fig is the leaf of moraceae plants banyan Ficusmicrocarpa.There is heat-clearing deliver, detoxify and promote the subsdence of swelling, effect of damp-clearing pain-relieving.
Whiterot fungi (Phanerochaetechrysosporium), bacterium number is: CICC14076.
Healthy and free from worry wood mould (Trichodermakoningii), bacterium number is: CICC40108
In prior art, not yet useful Natant Saluinia Herb and Leaf of Smallfruit Fig are the report that main raw material prepares organic fertilizer, more not with the report of this fertilizer control sclerotinia rot of colza.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind ofly can prevent and treat sclerotinia rot of colza and the organic fertilizer of fertility excellence.
In order to solve the problems of the technologies described above, the present invention proposes following technical proposal:
The organic fertilizer that Natant Saluinia Herb and Leaf of Smallfruit Fig are made, is characterized in that, described organic fertilizer take mass ratio as raw material based on the Natant Saluinia Herb of 2:1 and Leaf of Smallfruit Fig, adopts solid fermentation method preparation and obtain.
Described solid fermentation method comprises the following steps:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: get described basic material, pulverize, insert 70-90 DEG C of baking oven inner drying, in every 100g basic material add 400-600g water, 0.2-0.8g glucose, 0.5g ferrous sulfate, 0.5g urea ratio add water, glucose, ferrous sulfate and urea, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 20-40mL in every 100g basic material is inoculated, and cultivates 8-12 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Strain expanded culture liquid PDA substratum used is the PDA substratum not adding agar, its preparation method takes 200g potato, clean peeling is cut into small pieces, the 1000ml that adds water boils half hour or steaming and decocting under high pressure 20 minutes, filtered through gauze, then add 10-20g glucose, filtered through gauze while hot after fully dissolving, packing test tube, after about sterilizing, taking-up storage is for subsequent use.
Preferably, described (2) step, the drying temperature of basic material is 80 DEG C.
Preferably, described (2) step, the quality that every 100g basic material adds water is 500g.
Preferably, described (2) step, the quality that every 100g basic material adds glucose is 0.5g.
Preferably, described (3) step, the volume that every 100g basic material adds containing bacterium liquid is 30mL.
Beneficial effect of the present invention can be proved by following test
Test example 1 manure trial
Material and reagent
Organic fertilizer 1 (being designated as examination group 1) of the present invention, prepares by embodiment 1; Organic fertilizer 2 (being designated as examination group 2) of the present invention, prepares by embodiment 2; Organic fertilizer 3 (being designated as examination group 3) of the present invention, prepares by embodiment 3.
Shanghai heat resistanceheat resistant 605 green vegetables seed.
Test method
Adopt randomized block design method design pot experiment.Get volume identical, the flowerpot that shape is consistent, be divided into for examination group (1,2,3) and blank group.For trying fertilizer according to clay fertilizer than 7:3 and mixing with soil, will irrigate with water after loading flowerpot, as supplying examination group (1,2,3); Blank group does not add fertilizer.Often organize test 10 basin, every basin is sowed 9 Shanghai heat resistanceheat resistant 605 seeds and (is considered the percentage of germination of seed, the point that 3 spacing are identical is fixed in flowerpot, each point puts 3 seeds, thinning after germinateing, each point only retains one and grows, and the consistent seedling of growing way), after planting sprinkle one deck dirt (form through high-temperature firing, prevent seed contamination) alive.
Cultivation Shanghai heat resistanceheat resistant 605 green vegetables of 40 days are plucked and weighs, record the growing state of each group of Shanghai heat resistanceheat resistant 605 green vegetables.
Test-results
Test-results is in table 1, and result shows, organic fertilizer of the present invention can improve the output of crop.
The impact that table 1 organic fertilizer of the present invention grows Shanghai heat resistanceheat resistant 605 green vegetables
| Process | Plant height (cm) | Strain heavy (g) | Degree of development (cm) | The number of blade |
| For examination group 1 | 25.9 | 87.4 | 25.3 | 13.1 |
| For examination group 2 | 24.5 | 88.7 | 25.7 | 12.9 |
| For examination group 3 | 26.2 | 90.2 | 26.3 | 13.7 |
| Blank group | 21.7 | 80.3 | 21.4 | 10.2 |
Test example 2 is on the impact of rape sclerotinite sclerotial germination rate
Material and reagent
Organic fertilizer 1 (being designated as examination group 1) of the present invention, prepares by embodiment 1; Organic fertilizer 2 (being designated as examination group 2) of the present invention, prepares by embodiment 2; Organic fertilizer 3 (being designated as examination group 3) of the present invention, prepares by embodiment 3.
Rape sclerotinite (Sclerotiniasclerotiorum (Lib.) deBary).
Test method
Adopt randomized block design method design pot experiment.Get volume identical, the flowerpot that shape is consistent, be divided into for examination group (1,2,3) and blank group.For trying fertilizer according to clay fertilizer than 7:3 and mixing with soil, will irrigate with water after loading flowerpot, as supplying examination group (1,2,3); Blank group does not add fertilizer.Often organize test 3 basin, load 21 sclerotinia rot of colza sclerotium, length of embedment 2cm with the little mesh bag 5cm × 5cm of nylon, when 60 days, measure the germination rate of sclerotium with potato sucrose substratum (PDA).
Test-results
Test-results is in table 2, and result shows, organic fertilizer of the present invention significantly can reduce the germination rate of rape nuclear disk sclerotium, has certain preventive and therapeutic effect to sclerotinia rot of colza.
Table 2 organic fertilizer of the present invention is on the impact of nuclear disk sclerotium germination rate
| Process | Germination rate (%) |
| For examination group 1 | 20.7 |
| For examination group 2 | 23.4 |
| For examination group 3 | 21.9 |
| Control group | 36.6 |
In order to better set forth technical scheme of the present invention, below in conjunction with embodiment, the present invention is further illustrated, but protection domain of the presently claimed invention is not limited to the following example.
Embodiment
Embodiment 1
The preparation method of organic fertilizer is as follows:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: take 2 parts of Natant Saluinia Herbs and 1 part of Leaf of Smallfruit Fig by quality, pulverize, insert 70 DEG C of baking oven inner dryings, in every 100g basic material add 400g water, 0.2g glucose, 0.5g ferrous sulfate, 0.5g urea ratio add water, glucose, ferrous sulfate and urea, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 20mL in every 100g basic material is inoculated, cultivates 8 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Embodiment 2
The preparation method of organic fertilizer is as follows:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: take 2 parts of Natant Saluinia Herbs and 1 part of Leaf of Smallfruit Fig by quality, pulverize, insert 90 DEG C of baking oven inner dryings, in every 100g basic material add 600g water, 0.8g glucose, 0.5g ferrous sulfate, 0.5g urea ratio add water, glucose, ferrous sulfate and urea, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 40mL in every 100g basic material is inoculated, cultivates 12 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Embodiment 3
The preparation method of organic fertilizer is as follows:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: take 2 parts of Natant Saluinia Herbs and 1 part of Leaf of Smallfruit Fig by quality, pulverize, insert 80 DEG C of baking oven inner dryings, in every 100g basic material add 500g water, 0.5g glucose, 0.5g ferrous sulfate, 0.5g urea ratio add water, glucose, ferrous sulfate and urea, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 30mL in every 100g basic material is inoculated, cultivates 10 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
Claims (6)
1. with the organic fertilizer that Natant Saluinia Herb and Leaf of Smallfruit Fig are made, it is characterized in that, described organic fertilizer take mass ratio as raw material based on the Natant Saluinia Herb of 2:1 and Leaf of Smallfruit Fig, adopts solid fermentation method preparation and obtain.
2. a kind of organic fertilizer according to claim 1, it is characterized in that, described solid fermentation method comprises the following steps:
(1) strain expanded culture: get the mould and whiterot fungi of healthy and free from worry wood, be inoculated in liquid PDA substratum, shaking table is cultivated and is expanded, obtained containing bacterium liquid;
(2) solid medium preparation: get described basic material, pulverize, insert 70-90 DEG C of baking oven inner drying, in every 100g basic material add 400-600g water, 0.2-0.8g glucose, 0.5g ferrous sulfate, 0.5g urea ratio add water, glucose, ferrous sulfate and urea, mix, as solid medium;
(3) solid fermentation: the ratio added containing bacterium liquid described in 20-40mL in every 100g basic material is inoculated, and cultivates 8-12 days under 28-35 DEG C of condition;
(4) sterilizing, drying: get (3) step gained material, autoclaving, insert 100 DEG C of baking oven inner dryings, to obtain final product.
3. a kind of organic fertilizer according to claim 2, it is characterized in that, described (2) step, the drying temperature of basic material is 80 DEG C.
4. a kind of organic fertilizer according to claim 2, it is characterized in that, described (2) step, the quality that every 100g basic material adds water is 500g.
5. a kind of organic fertilizer according to claim 2, it is characterized in that, described (2) step, the quality that every 100g basic material adds glucose is 0.5g.
6. a kind of organic fertilizer according to claim 2, it is characterized in that, described (3) step, the volume that every 100g basic material adds containing bacterium liquid is 30mL.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510447597.9A CN105110827A (en) | 2015-07-24 | 2015-07-24 | Organic fertilizer prepared from Salvinia nutans and leaf of Ficus microcarpa |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510447597.9A CN105110827A (en) | 2015-07-24 | 2015-07-24 | Organic fertilizer prepared from Salvinia nutans and leaf of Ficus microcarpa |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103819236A (en) * | 2014-03-06 | 2014-05-28 | 西双版纳天源林石斛种植有限公司 | Fermentation substrate as well as preparation method and application thereof |
| CN104016813A (en) * | 2014-06-06 | 2014-09-03 | 茹晖 | Ecological pesticide-added fertilizer and preparation method thereof |
| CN104276896A (en) * | 2014-10-24 | 2015-01-14 | 国家粳稻工程技术研究中心 | Ecological medicinal fertilizer for rice and preparation method of ecological medicinal fertilizer |
| CN104557332A (en) * | 2014-12-19 | 2015-04-29 | 宿州腾飞有机肥料有限公司 | Wheat straw/chicken manure fermented organic fertilizer and preparation method thereof |
-
2015
- 2015-07-24 CN CN201510447597.9A patent/CN105110827A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103819236A (en) * | 2014-03-06 | 2014-05-28 | 西双版纳天源林石斛种植有限公司 | Fermentation substrate as well as preparation method and application thereof |
| CN104016813A (en) * | 2014-06-06 | 2014-09-03 | 茹晖 | Ecological pesticide-added fertilizer and preparation method thereof |
| CN104276896A (en) * | 2014-10-24 | 2015-01-14 | 国家粳稻工程技术研究中心 | Ecological medicinal fertilizer for rice and preparation method of ecological medicinal fertilizer |
| CN104557332A (en) * | 2014-12-19 | 2015-04-29 | 宿州腾飞有机肥料有限公司 | Wheat straw/chicken manure fermented organic fertilizer and preparation method thereof |
Non-Patent Citations (3)
| Title |
|---|
| 司华等: "油菜茵核病田间残留菌核萌发规律及研究初报", 《汉中科技》 * |
| 曹翠玲等: "康氏木霉代谢物对植物病原真菌的抗菌活性测定", 《陕西农业科学》 * |
| 谢立华等: "促进作物秸秆和菌核腐解的复合生物制剂应用效果", 《中国油料作物学报》 * |
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