CN105076251A - Preparation method of natural mildew fungicide - Google Patents
Preparation method of natural mildew fungicide Download PDFInfo
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- CN105076251A CN105076251A CN201510576136.1A CN201510576136A CN105076251A CN 105076251 A CN105076251 A CN 105076251A CN 201510576136 A CN201510576136 A CN 201510576136A CN 105076251 A CN105076251 A CN 105076251A
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Abstract
The invention relates to a preparation method of a natural mildew fungicide, and belongs to the technical field of daily fungicide preparation methods. The method comprises the following steps: with hawthorns, green apples, sour plums and seawater as raw materials, firstly fermenting the hawthorns, the green apples and the sour plums, and filtering to obtain a sour liquid; mixing the obtained sour liquid with the concentrated seawater; and finally refining lilac essential oil, and mixing with the mixed liquid of the sour liquid and seawater, so as to prepare the natural mildew fungicide. An experiment proves that the method provided by the invention is unique and novel, does not contain any chemical component harmful to a human body, is safe and healthful, is capable of effectively killing insects, can be placed in any place, and is free of space limitation, and significant in sterilization effect; and the bacteria removing rate can be up to over 90%.
Description
Technical field
The present invention relates to a kind of preparation method of the natural mould bactericide that goes out, belong to daily sterilization preparation method technical field.
Background technology
Mould is the whole hyphomycetes not forming large fruit body in fungi, often colonizes on animal and plant body and carries out flourish.Owing to there is many fungal spores in air, after falling within the surfaces such as food, medicinal material, leather and fur products, grain and feed, namely sprout under suitable temperature and humidity for mycelia, thus secrete the tissue of enzyme corrosion above-mentioned substance, so that they are rotten rotten, and effect, nutrient component change.
Along with the raising of people's living standard and the continuous improvement of quality of life, people require more and more higher to the well-formedness of daily necessities, health, safety and the feature of environmental protection etc., one of antibacterial popular vocabulary becoming this century.Nowadays antibacterial mainly by adding antibacterial matrices or place the product of anti-mould and anti-moth when storing in manufacturing process, its mainly by camphor, naphthalene, paracide etc. for effective ingredient is made.Wherein the fungi-proofing mothproofing agent that uses as earliest period of naphthalene, due to its toxicity and carcinogenesis, is ordering to have prohibitted the use, the dichloro-benzenes of next very early, although performance property is good, it has carcinogenic effect equally, cannot be used as food mildew-resistant; Although other camphor safety, cannot reach mothproof standard does not have significant mildew-proof function yet.
Chemical antibacterial mildew inhibitor conventional at present in addition produces inhibitory action for a certain link of mould biological metabolism with selecting row often, very easily causes the formation and development of microbial resistance thus.A lot of research demonstrates most of mould and creates resistance for existing chemical anti mildew agent, and, a large amount of uses of chemical anti mildew agent bring serious ecological environmental pollution problem to the whole world, its residual toxicity has also become the hidden trouble of food security simultaneously, in addition, chemosynthesis, containing a large amount of not biodegradable compositions.So invention one both can be degraded, and had without any toxicity, and the significant antibacterial agent of antibacterial effect, be thing extremely urgent at present.
Summary of the invention
The technical problem that the present invention mainly solves: the antibacterial camphor ball commonly used for current people in use, there is poison gas by release of slowly volatilizing thus kill mould and insect, but it contains a large amount of carcinogenic components, very large to harm, and only to be placed on sterilization debug in cupboard, the drawback of food aspect cannot be used for, provide a kind of preparation method of the natural mould bactericide that goes out, the method is by hawthorn, green apple, acid Lee, seawater is as raw material, first pass through hawthorn, green apple, the fermentation of acid lijin row, filter to obtain acid solution, afterwards the acid solution obtained and the seawater concentrate after concentrating are mixed, finally by refinement lilac essential oil, allow itself and acid solution, sea water mixing liquid is mixed together, thus the obtained natural mould bactericide that goes out of one of the present invention.This natural sterilizing bactericide, the chemical composition not containing harmful to human, not only safety, health, manufacture process is simple, and bactericidal effect is remarkable, can serve as the bactericide in any field, make degerming rate up to more than 90%.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
(1) count by weight, get 20 ~ 30 portions of fresh hawthorn, 30 ~ 55 parts of green apples, 35 ~ 50 parts of sour Lee, add 5 ~ 10g soda ash in clear water, mix, wash by rubbing with the hands, and stoning, smash rear slagging to pieces;
(2) pomace obtained after above-mentioned smashing to pieces is put into fermentation tank, be placed in the place of ventilation, lucifuge, and add 500 ~ 600mL deionized water fermentation process is carried out to it, first fermentation temperature is regulated to be 20 ~ 22 DEG C, sealing and fermenting 15 ~ 18 days, after to heighten fermentation temperature be 38 ~ 55 DEG C, sealing and fermenting 17 ~ 20 days, must mix turbid liquid;
(3) the turbid liquid of mixing taken out in above-mentioned fermentation tank is positioned in centrifuge, and centrifugal rotational speed is 4000 ~ 5000r/min, and under 20 ~ 25 DEG C of conditions, centrifugal treating 10 ~ 15min, obtains mix acid liquor, and to survey its pH value be 1.1 ~ 1.4 to be advisable;
(4) a 2L airtight container is got, separate from centre with a semipermeable membrane, be divided into two-layer up and down, pour 800 ~ 900mL seawater on semipermeable membrane upper strata, pressurize afterwards to container top, pressure is set to 1 ~ 2MPa, semipermeable membrane upper liquid is got after pressurization 10 ~ 15min, and fully mix with mix acid liquor obtained above, wherein said semipermeable membrane only allows seawater to pass through, and sodium chloride then cannot pass through;
(5) lilac essential oil obtained with refinement for the mixed liquor of above-mentioned gained is fully mixed, a kind of natural mould bactericide that goes out can be obtained.
Described lilac essential oil refining technique step is:
(1) gathering fresh lilac, to be soaked in mass concentration be in the acetone soln of 70%, 1 ~ 2h is stirred at 45 ~ 65 DEG C, be positioned in high speed disintegrator after cooling and pulverize, and to be soaked in mass concentration be in the acetone soln of 55%, carry out microwave abstracting 5 ~ 10min, microwave power is 250 ~ 450w, cooled and filtered goes out precipitation, collect extract, put it in concentration tank and be decompressed to 900 ~ 1000Pa, obtain concentrate;
(2) concentrate obtained above is put into distillation equipment, add 300 ~ 400mL distilled water, oil bath is warming up to 100 ~ 110 DEG C, refluxes, and collects distillation fraction, naturally cools;
(3) the above-mentioned distillation fraction collected is cooled to 3 ~ 7 DEG C, joining mass ratio is in the ethyl acetate of 1:5 ~ 1:10 and the mixed liquor of water, be placed on magnetic stirrer, under the rotating speed of 100 ~ 200r/min, stratification after stirring 10 ~ 15min, is separated and obtains organic layer, add anhydrous calcium chloride and stir in organic layer, leave standstill, filter, after removing precipitation, filtrate is put into concentration tank and be decompressed to 800 ~ 900Pa, the concentrate obtained is lilac essential oil.
Principle of the present invention is: natural the going out mould dose obtained after extraction volatilized, and in volatilization process, organic matter exists acid and salt component, acid and salt is filled the air in atmosphere, thus restrained effectively growing of mould, reach mould effect of going out.
Application method of the present invention is: during use, by the natural mould bactericide that goes out obtained by the present invention, add in the vial after joining sterilization after water dilutes 100 times, be placed on any place needing sterilization sterilizing, will tell on after general 15 ~ 25min, not only effectively can kill insect, and sterilization effect is remarkable, makes degerming rate up to more than 90%.
The invention has the beneficial effects as follows:
(1) chemical composition not containing harmful to human, safety, health, can serve as the bactericide in any field;
(2) broken away from current bactericide and mostly carried out sterilization to be placed in cupboard only, made can place Anywhere, without limitations;
(3) bactericidal effect is remarkable, and degerming rate is up to more than 90%.
Embodiment
First count by weight, get 20 ~ 30 portions of fresh hawthorn, 30 ~ 55 parts of green apples, 35 ~ 50 parts of sour Lee, add 5 ~ 10g soda ash in clear water, mix, wash by rubbing with the hands, and stoning, smash rear slagging to pieces; Next the pomace obtained after above-mentioned smashing to pieces is put into fermentation tank, be placed in the place of ventilation, lucifuge, and add 500 ~ 600mL deionized water fermentation process is carried out to it, first fermentation temperature is regulated to be 20 ~ 22 DEG C, sealing and fermenting 15 ~ 18 days, after to heighten fermentation temperature be 38 ~ 55 DEG C, sealing and fermenting 17 ~ 20 days, must mix turbid liquid;
The turbid liquid of mixing taken out subsequently in above-mentioned fermentation tank is positioned in centrifuge, and centrifugal rotational speed is 4000 ~ 5000r/min, centrifugal treating 10 ~ 15min under 20 ~ 25 DEG C of conditions, and obtaining mix acid liquor and surveying its pH value is 1.1 ~ 1.4 to be advisable; Get a 2L airtight container afterwards again, separate from centre with a semipermeable membrane, be divided into two-layer up and down, pour 800 ~ 900mL seawater on semipermeable membrane upper strata, pressurize afterwards to container top, pressure is set to 1 ~ 2MPa, semipermeable membrane upper liquid is got after pressurization 10 ~ 15min, and fully mix with mix acid liquor obtained above, wherein said semipermeable membrane only allows seawater to pass through, and sodium chloride then cannot pass through;
Finally lilac essential oil obtained with refinement for the mixed liquor of above-mentioned gained is fully mixed, a kind of natural mould bactericide that goes out can be obtained.Wherein said lilac essential oil refining technique step is: first gathering fresh lilac, to be soaked in mass concentration be in the acetone soln of 70%, 1 ~ 2h is stirred at 45 ~ 65 DEG C, be positioned in high speed disintegrator after cooling and pulverize, and to be soaked in mass concentration be in the acetone soln of 55%, carry out microwave abstracting 5 ~ 10min, microwave power is 250 ~ 450w, cooled and filtered goes out precipitation, collect extract, put it in concentration tank and be decompressed to 900 ~ 1000Pa, obtain concentrate; Then concentrate obtained above is put into distillation equipment, add 300 ~ 400mL distilled water, oil bath is warming up to 100 ~ 110 DEG C, refluxes, and collects distillation fraction, naturally cools; Finally the above-mentioned distillation fraction collected is cooled to 3 ~ 7 DEG C, joining mass ratio is in the ethyl acetate of 1:5 ~ 1:10 and the mixed liquor of water, be placed on magnetic stirrer, under the rotating speed of 100 ~ 200r/min, stratification after stirring 10 ~ 15min, is separated and obtains organic layer, add anhydrous calcium chloride and stir in organic layer, leave standstill, filter, after removing precipitation, filtrate is put into concentration tank and be decompressed to 800 ~ 900Pa, the concentrate obtained is lilac essential oil.
Example 1
First count by weight, get 20 portions of fresh hawthorn, 30 parts of green apples, 35 parts of sour Lee, add 5g soda ash in clear water, mix, wash by rubbing with the hands, and stoning, smash rear slagging to pieces; Next the pomace obtained after above-mentioned smashing to pieces is put into fermentation tank, be placed in the place of ventilation, lucifuge, and add 500mL deionized water fermentation process is carried out to it, first regulate fermentation temperature to be 20 DEG C, sealing and fermenting 15 days, after to heighten fermentation temperature be 38 DEG C, sealing and fermenting 17 days, must mix turbid liquid; The turbid liquid of mixing taken out subsequently in above-mentioned fermentation tank is positioned in centrifuge, and centrifugal rotational speed is 4000r/min, and centrifugal treating 10min under 20 DEG C of conditions, obtains mix acid liquor, and to survey its pH value be 1.1 to be advisable; Get a 2L airtight container afterwards, separate from centre with a semipermeable membrane, be divided into two-layer up and down, pour 800mL seawater on semipermeable membrane upper strata, pressurize afterwards to container top, pressure is set to 1MPa, semipermeable membrane upper liquid is got after pressurization 10min, and fully mix with mix acid liquor obtained above, wherein said semipermeable membrane only allows seawater to pass through, and sodium chloride then cannot pass through; Finally lilac essential oil obtained with refinement for the mixed liquor of above-mentioned gained is fully mixed, a kind of natural mould bactericide that goes out can be obtained.Wherein said lilac essential oil refining technique step is: first gathering fresh lilac, to be soaked in mass concentration be in the acetone soln of 70%, 1h is stirred at 45 DEG C, be positioned in high speed disintegrator after cooling and pulverize, and to be soaked in mass concentration be in the acetone soln of 55%, carry out microwave abstracting 5min, microwave power is 250w, cooled and filtered goes out precipitation, collect extract, put it in concentration tank and be decompressed to 900Pa, obtain concentrate; Then concentrate obtained above is put into distillation equipment, add 300mL distilled water, oil bath is warming up to 100 DEG C, refluxes, and collects distillation fraction, naturally cools; Finally the above-mentioned distillation fraction collected is cooled to 3 DEG C, joining mass ratio is in the ethyl acetate of 1:5 and the mixed liquor of water, be placed on magnetic stirrer, stratification stir 10min under the rotating speed of 100r/min after, is separated and obtains organic layer, add anhydrous calcium chloride and stir in organic layer, leave standstill, filter, after removing precipitation, filtrate is put into concentration tank and be decompressed to 800Pa, the concentrate obtained is lilac essential oil.
When this example uses, by the natural mould bactericide that goes out obtained by the present invention, add in the vial after joining sterilization after water dilutes 100 times, be placed on any place needing sterilization sterilizing, will tell on after general 15min, not only effectively can kill insect, and sterilization effect is remarkable, makes degerming rate up to 90%.
Example 2
First count by weight, get 25 portions of fresh hawthorn, 40 parts of green apples, 45 parts of sour Lee, add 8g soda ash in clear water, mix, wash by rubbing with the hands, and stoning, smash rear slagging to pieces; Next the pomace obtained after above-mentioned smashing to pieces is put into fermentation tank, be placed in the place of ventilation, lucifuge, and add 550mL deionized water fermentation process is carried out to it, first regulate fermentation temperature to be 21 DEG C, sealing and fermenting 17 days, after to heighten fermentation temperature be 48 DEG C, sealing and fermenting 18 days, must mix turbid liquid; The turbid liquid of mixing taken out subsequently in above-mentioned fermentation tank is positioned in centrifuge, and centrifugal rotational speed is 4500r/min, and centrifugal treating 13min under 22 DEG C of conditions, obtains mix acid liquor, and to survey its pH value be 1.2 to be advisable; Get a 2L airtight container afterwards, separate from centre with a semipermeable membrane, be divided into two-layer up and down, pour 850mL seawater on semipermeable membrane upper strata, pressurize afterwards to container top, pressure is set to 1.5MPa, semipermeable membrane upper liquid is got after pressurization 13min, and fully mix with mix acid liquor obtained above, wherein said semipermeable membrane only allows seawater to pass through, and sodium chloride then cannot pass through; Finally lilac essential oil obtained with refinement for the mixed liquor of above-mentioned gained is fully mixed, a kind of natural mould bactericide that goes out can be obtained.Wherein said lilac essential oil refining technique step is: first gathering fresh lilac, to be soaked in mass concentration be in the acetone soln of 70%, 1.5h is stirred at 50 DEG C, be positioned in high speed disintegrator after cooling and pulverize, and to be soaked in mass concentration be in the acetone soln of 55%, carry out microwave abstracting 8min, microwave power is 350w, cooled and filtered goes out precipitation, collect extract, put it in concentration tank and be decompressed to 950Pa, obtain concentrate; Then concentrate obtained above is put into distillation equipment, add 350mL distilled water, oil bath is warming up to 105 DEG C, refluxes, and collects distillation fraction, naturally cools; Finally the above-mentioned distillation fraction collected is cooled to 6 DEG C, joining mass ratio is in the ethyl acetate of 1:7 and the mixed liquor of water, be placed on magnetic stirrer, stratification stir 13min under the rotating speed of 150r/min after, is separated and obtains organic layer, add anhydrous calcium chloride and stir in organic layer, leave standstill, filter, after removing precipitation, filtrate is put into concentration tank and be decompressed to 850Pa, the concentrate obtained is lilac essential oil.
This example in use, by the natural mould bactericide that goes out obtained by the present invention, add in the vial after joining sterilization after water dilutes 70 times, be placed on any place needing sterilization sterilizing, will tell on after general 20min, not only effectively can kill insect, and sterilization effect is remarkable, makes degerming rate up to 92%.
Example 3
First count by weight, get 30 portions of fresh hawthorn, 55 parts of green apples, 50 parts of sour Lee, add 10g soda ash in clear water, mix, wash by rubbing with the hands, and stoning, smash rear slagging to pieces; Next the pomace obtained after above-mentioned smashing to pieces is put into fermentation tank, be placed in the place of ventilation, lucifuge, and add 600mL deionized water fermentation process is carried out to it, first regulate fermentation temperature to be 22 DEG C, sealing and fermenting 18 days, after to heighten fermentation temperature be 55 DEG C, sealing and fermenting 20 days, must mix turbid liquid; The turbid liquid of mixing taken out subsequently in above-mentioned fermentation tank is positioned in centrifuge, and centrifugal rotational speed is 5000r/min, and centrifugal treating 15min under 25 DEG C of conditions, obtains mix acid liquor, and to survey its pH value be 1.4 to be advisable; Get a 2L airtight container afterwards, separate from centre with a semipermeable membrane, be divided into two-layer up and down, pour 900mL seawater on semipermeable membrane upper strata, pressurize afterwards to container top, pressure is set to 2MPa, semipermeable membrane upper liquid is got after pressurization 15min, and fully mix with mix acid liquor obtained above, wherein said semipermeable membrane only allows seawater to pass through, and sodium chloride then cannot pass through; Finally lilac essential oil obtained with refinement for the mixed liquor of above-mentioned gained is fully mixed, a kind of natural mould bactericide that goes out can be obtained.Wherein said lilac essential oil refining technique step is: first gathering fresh lilac, to be soaked in mass concentration be in the acetone soln of 70%, 2h is stirred at 65 DEG C, be positioned in high speed disintegrator after cooling and pulverize, and to be soaked in mass concentration be in the acetone soln of 55%, carry out microwave abstracting 10min, microwave power is 450w, cooled and filtered goes out precipitation, collect extract, put it in concentration tank and be decompressed to 1000Pa, obtain concentrate; Then concentrate obtained above is put into distillation equipment, add 400mL distilled water, oil bath is warming up to 110 DEG C, refluxes, and collects distillation fraction, naturally cools; Finally the above-mentioned distillation fraction collected is cooled to 7 DEG C, joining mass ratio is in the ethyl acetate of 1:10 and the mixed liquor of water, be placed on magnetic stirrer, stratification stir 15min under the rotating speed of 200r/min after, is separated and obtains organic layer, add anhydrous calcium chloride and stir in organic layer, leave standstill, filter, after removing precipitation, filtrate is put into concentration tank and be decompressed to 900Pa, the concentrate obtained is lilac essential oil.
This example in use, by the natural mould bactericide that goes out obtained by the present invention, add in the vial after joining sterilization after water dilutes 50 times, be placed on any place needing sterilization sterilizing, will tell on after general 25min, not only effectively can kill insect, and sterilization effect is remarkable, makes degerming rate up to 94%.
Claims (2)
1. a preparation method for the natural mould bactericide that goes out, is characterized in that concrete preparation process is:
(1) count by weight, get 20 ~ 30 portions of fresh hawthorn, 30 ~ 55 parts of green apples, 35 ~ 50 parts of sour Lee, add 5 ~ 10g soda ash in clear water, mix, wash by rubbing with the hands, and stoning, smash rear slagging to pieces;
(2) pomace obtained after above-mentioned smashing to pieces is put into fermentation tank, be placed in the place of ventilation, lucifuge, and add 500 ~ 600mL deionized water fermentation process is carried out to it, first fermentation temperature is regulated to be 20 ~ 22 DEG C, sealing and fermenting 15 ~ 18 days, after to heighten fermentation temperature be 38 ~ 55 DEG C, sealing and fermenting 17 ~ 20 days, must mix turbid liquid;
(3) the turbid liquid of mixing taken out in above-mentioned fermentation tank is positioned in centrifuge, and centrifugal rotational speed is 4000 ~ 5000r/min, and under 20 ~ 25 DEG C of conditions, centrifugal treating 10 ~ 15min, obtains mix acid liquor, and to survey its pH value be 1.1 ~ 1.4 to be advisable;
(4) a 2L airtight container is got, separate from centre with a semipermeable membrane, be divided into two-layer up and down, pour 800 ~ 900mL seawater on semipermeable membrane upper strata, pressurize afterwards to container top, pressure is set to 1 ~ 2MPa, semipermeable membrane upper liquid is got after pressurization 10 ~ 15min, and fully mix with mix acid liquor obtained above, wherein said semipermeable membrane only allows seawater to pass through, and sodium chloride then cannot pass through;
(5) lilac essential oil obtained with refinement for the mixed liquor of above-mentioned gained is fully mixed, a kind of natural mould bactericide that goes out can be obtained.
2. the preparation method of a kind of natural mould bactericide that goes out according to claim 1, is characterized in that: described lilac essential oil refining technique step is:
(1) gathering fresh lilac, to be soaked in mass concentration be in the acetone soln of 70%, 1 ~ 2h is stirred at 45 ~ 65 DEG C, be positioned in high speed disintegrator after cooling and pulverize, and to be soaked in mass concentration be in the acetone soln of 55%, carry out microwave abstracting 5 ~ 10min, microwave power is 250 ~ 450w, cooled and filtered goes out precipitation, collect extract, put it in concentration tank and be decompressed to 900 ~ 1000Pa, obtain concentrate;
(2) concentrate obtained above is put into distillation equipment, add 300 ~ 400mL distilled water, oil bath is warming up to 100 ~ 110 DEG C, refluxes, and collects distillation fraction, naturally cools;
(3) the above-mentioned distillation fraction collected is cooled to 3 ~ 7 DEG C, joining mass ratio is in the ethyl acetate of 1:5 ~ 1:10 and the mixed liquor of water, be placed on magnetic stirrer, under the rotating speed of 100 ~ 200r/min, stratification after stirring 10 ~ 15min, is separated and obtains organic layer, add anhydrous calcium chloride and stir in organic layer, leave standstill, filter, after removing precipitation, filtrate is put into concentration tank and be decompressed to 800 ~ 900Pa, the concentrate obtained is lilac essential oil.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105902721A (en) * | 2016-05-04 | 2016-08-31 | 云南人本生物科技有限公司 | Enzyme disinfectant |
| CN111345325A (en) * | 2020-02-26 | 2020-06-30 | 山西大学 | Method for preparing disinfectant by using Chinese herbal medicine brewing technology |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86103583A (en) * | 1986-05-15 | 1987-05-20 | 国家海洋局第三海洋研究所 | Bamboo and wood moth-proof and mould-proof inorganic agent |
| CN1089509A (en) * | 1993-01-09 | 1994-07-20 | 石家庄开发区科润科技发展公司 | Fridge freshness retaining eliminate the unusual smell spray and manufacturing process |
| CN1147899A (en) * | 1995-10-19 | 1997-04-23 | 林添波 | Agent for preservation of Lichi fruit |
| AU785415B2 (en) * | 1999-07-14 | 2007-05-03 | Hurstwell Pty Ltd | A nematicide composition |
| CN102793264A (en) * | 2012-08-31 | 2012-11-28 | 中国烟草总公司郑州烟草研究院 | Preparation method of magnolia fruit extract and application thereof in cigarette |
-
2015
- 2015-09-11 CN CN201510576136.1A patent/CN105076251A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN86103583A (en) * | 1986-05-15 | 1987-05-20 | 国家海洋局第三海洋研究所 | Bamboo and wood moth-proof and mould-proof inorganic agent |
| CN1089509A (en) * | 1993-01-09 | 1994-07-20 | 石家庄开发区科润科技发展公司 | Fridge freshness retaining eliminate the unusual smell spray and manufacturing process |
| CN1147899A (en) * | 1995-10-19 | 1997-04-23 | 林添波 | Agent for preservation of Lichi fruit |
| AU785415B2 (en) * | 1999-07-14 | 2007-05-03 | Hurstwell Pty Ltd | A nematicide composition |
| CN102793264A (en) * | 2012-08-31 | 2012-11-28 | 中国烟草总公司郑州烟草研究院 | Preparation method of magnolia fruit extract and application thereof in cigarette |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105902721A (en) * | 2016-05-04 | 2016-08-31 | 云南人本生物科技有限公司 | Enzyme disinfectant |
| CN111345325A (en) * | 2020-02-26 | 2020-06-30 | 山西大学 | Method for preparing disinfectant by using Chinese herbal medicine brewing technology |
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Application publication date: 20151125 |