CN105055539A - Chinese herb extract with antibacterial effect and preparation method for Chinese herb extract - Google Patents
Chinese herb extract with antibacterial effect and preparation method for Chinese herb extract Download PDFInfo
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Abstract
The invention discloses a Chinese herb extract with an antibacterial effect. The Chinese herb extract is prepared by decocting the following raw materials in parts by weight with water and performing alcohol extraction: 120-180 parts of ciderage, 120-180 parts of pogostemon cablin, 120-180 parts of glossy privet fruit and 80-120 parts of astragalus membranaceus. An in-vitro antibacterial test result shows that the Chinese herb extract disclosed by the invention is good in antibacterial effect and has broad spectrum activity; compared with a water extract, a microwave extract and an ethanol fractionated extract, the Chinese herb extract is better in antibacterial effect. The Chinese herb extract is used as an antibacterial medicament for poultry substitute antibiotics and chemical medicaments, so that pollution, caused by drug resistance and drug retaining, to a poultry product can be effectively avoided; the poultry harmful excrements, the protein use amount and the nitrogen pollution are reduced; the Chinese herb extract has a wide market prospect.
Description
Technical field
The invention belongs to antibacterials technical field, particularly relate to a kind of Chinese herbal medicine extract with antibacterial action and preparation method thereof.
Background technology
Along with the fast development of aquaculture, antibiotic application is more and more extensive, and it serves certain positive role in Animal diseases prevention, treatment etc.But in the last few years, the drug resistance problems caused by the abuse of antibiotic in aquaculture can not be ignored.And, long-term a large amount of not scientifical use antibiotic also can make animal wastes contain heavy-metal residual, causes environmental pollution, brings serious harm to human health, and the develop rapidly of aquaculture not only increases the supply of animal product, also bring the problem of food safety simultaneously.The Chinese medicine compound of wide spectrum, low toxicity, noresidue is more and more extensive in the application of veterinary clinic, and the feature of its enhancing immunity, broad-spectrum antiseptic, growth promoting effects more and more obtains the favor of aquaculture.Therefore, research and develop a kind of there is antibacterial action, inexorable trend that nontoxic residue-free, alternative antibiotic herbal product are modern farming and social development, significant and using value.
Summary of the invention
The technical problem to be solved in the present invention is to provide the Chinese herbal medicine extract with antibacterial action and preparation method thereof of a kind of has a broad antifungal spectrum, nontoxic residue-free.
For solving the problems of the technologies described above, the present invention is by the following technical solutions: the Chinese herbal medicine extract with antibacterial action, is made up: Herba polygoni hydropiperis 120 ~ 180 parts, Herba Pogostemonis 120 ~ 180 parts of the raw medicinal material of following weight portion through decocting alcohol extraction, Fructus Ligustri Lucidi 120 ~ 180 parts, the Radix Astragali 80 ~ 120 parts.
The above-mentioned preparation method with the Chinese herbal medicine extract of antibacterial action, makes after each raw medicinal material mixing through decocting alcohol extraction.
Decocting alcohol extraction is undertaken by following operation: take each raw medicinal material 20g altogether by weight, pulverize, mixing, adds 10 times of soak by water 3 times, water temperature 100 DEG C, each 1.5 hours; Filter, after merging 3 filtrates, the centrifugal 30min of 3000r/min collects supernatant; Adding 95% ethanol after supernatant Rotary Evaporators is concentrated into small size makes ethanol content be 80%, filters and remove precipitation after leaving standstill 24h; Filtrate, to collect supernatant after the centrifugal 30min of 3000r/min, reclaims ethanol with Rotary Evaporators, and after gained medicinal liquid is settled to 20mL, 4 DEG C save backup.
For the problem of current aquaculture Chinese medicine abuse, inventor is theoretical for instructing with traditional Chinese veterinarian, prescription has been prepared a kind of compound recipe Herba polygoni hydropiperis and has been fallen apart, comprise the raw medicinal material of following weight portion: Herba polygoni hydropiperis 120 ~ 180 parts, Herba Pogostemonis 120 ~ 180 parts, Fructus Ligustri Lucidi 120 ~ 180 parts, the Radix Astragali 80 ~ 120 parts, has made a kind of Chinese herbal medicine extract with antibacterial action by the raw medicinal material of above weight portion through decocting alcohol extraction.Extracorporeal bacteria inhibitor test result shows, Chinese herbal medicine extract of the present invention has good antibacterial effect, has broad spectrum activity, and its antibacterial effect is better compared with water extract, microwave extraction thing, alcohol grading extract.Using the antibacterials of the present invention as birds substitute antibiotics and chemicals, Drug resistance and drug residue effectively can be avoided the pollution of livestock product, reduce poultry and be harmful to Excreta, enzyme protein dosage and polluted by nitrogen, there is wide market prospect.
Detailed description of the invention
Embodiment 1 has the preparation of the Chinese herbal medicine extract of antibacterial action
Formula: Herba polygoni hydropiperis 120 ~ 180 parts, Herba Pogostemonis 120 ~ 180 parts, Fructus Ligustri Lucidi 120 ~ 180 parts, the Radix Astragali 80 ~ 120 parts.The concrete formula of each sample is in table 1.
Table 1 has the formula table of the Chinese herbal medicine extract of antibacterial action
| Sample formulations sequence number | Herba polygoni hydropiperis | Herba Pogostemonis | Fructus Ligustri Lucidi | The Radix Astragali |
| 1 | 120 parts | 145 parts | 180 parts | 80 parts |
| 2 | 180 parts | 120 parts | 125 parts | 120 parts |
| 3 | 150 parts | 180 parts | 165 parts | 95 parts |
| 4 | 120 parts | 120 parts | 120 parts | 80 parts |
Method for making: make after each raw medicinal material mixing through decocting alcohol extraction, decocting alcohol extraction is undertaken by following operation: take each raw medicinal material 20g altogether by weight, pulverize, mixing, adds 10 times of soak by water 3 times, water temperature 100 DEG C, each 1.5 hours; Filter, after merging 3 filtrates, the centrifugal 30min of 3000r/min collects supernatant; Adding 95% ethanol after supernatant Rotary Evaporators is concentrated into small size makes ethanol content be 80%, filters and remove precipitation after leaving standstill 24h; Filtrate, to collect supernatant after the centrifugal 30min of 3000r/min, reclaims ethanol with Rotary Evaporators, and after gained medicinal liquid is settled to 20mL, 4 DEG C save backup.
The preparation of embodiment 2 control sample
Water extract contrasts
Take each raw material 20g altogether by formula 4, pulverize, mixing, adds 10 times of soak by water 3 times, water temperature 100 DEG C, each 1.5 hours; Filter, after merging 3 filtrates, 3000r/min centrifugal 30min collection supernatant, supernatant Rotary Evaporators is concentrated and after being settled to 20ml 4 DEG C save backup.
Microwave extraction thing contrasts
Take each raw material altogether 20g by formula 4, pulverize, mixing, add after 10 times of water fully mix, be placed in microwave sample processing system 100 DEG C process 15min.
Alcohol grading extract contrasts
Take each raw material 20g altogether by formula 4, pulverize, mixing, add 95% of 10 times of volumes, 70%, 45% alcohol steep respectively 3 times, extraction time is 48h, 24h, 24h.
Embodiment 3 has the application pressure testing of the Chinese herbal medicine extract of antibacterial action
1. test material
Control sample in Example 1 in the sample (hereinafter referred to as compound recipe Herba polygoni hydropiperis decocting ethanol extract or decocting ethanol extract) of formula 4 and embodiment 2 is used for following test.
2. In Vitro Bacteriostasis effect measuring
The preparation of bacterium liquid: be inoculated in nutrient agar panel after fully being recovered by the test bacteria such as S. pullonum, chicken colibacillosis, staphylococcus aureus, Candida albicans and bacillus pyocyaneus, 24h is cultivated at 37 DEG C, picking colonies typical is inoculated in nutrient broth medium, 18h is cultivated, by bacterial concentration furnishing 3 × 10 at 37 DEG C
8individual/mL is for subsequent use.
Assay method: the In Vitro Bacteriostasis effect measuring each extract with paper disk method, with penicillin, streptomycin in contrast.Tested bacterium liquid is spread evenly across on the nutrient agar panel in Fen Hao district, slightly put a moment, the scraps of paper fully drawing each tested medicinal liquid are affixed on region central authorities, back-off plate, measure antibacterial circle diameter cultivate 12h ~ 18h at 37 DEG C after and record (the results are shown in Table 2).
Antibacterial intensity criterion: be judged to insensitive without inhibition zone; Antibacterial circle diameter is less than 10mm person and is judged to less sensitive; Between 10 ~ 15mm, person is judged to medium sensitivity; Between 16 ~ 20mm, person is judged to extremely sensitive; More than 20mm person is judged to extremely responsive.
Table 2 each extract In Vitro Bacteriostasis circle measurement result
As seen from the above table, S. pullonum is extremely sensitive to compound recipe Herba polygoni hydropiperis decocting ethanol extract; Chicken colibacillosis is extremely sensitive to compound recipe Herba polygoni hydropiperis decocting ethanol extract; Staphylococcus aureus is extremely sensitive to compound recipe Herba polygoni hydropiperis decocting ethanol extract; Candida albicans is to compound recipe Herba polygoni hydropiperis decocting ethanol extract less sensitive; Bacillus pyocyaneus is to compound recipe Herba polygoni hydropiperis decocting ethanol extract less sensitive.
3. the mensuration of minimal inhibitory concentration
The minimal inhibitory concentration MIC of above-mentioned each extract to S. pullonum, chicken colibacillosis, staphylococcus aureus, Candida albicans and bacillus pyocyaneus is measured with test tube two way classification.Sterile working adds 0.5mL nutrient broth in 1 to No. 10 EP pipe, draw each extract of 0.5mL and add No. 1 pipe, fully mix, from No. 1 pipe, draw 0.5mL liquid add No. 2 pipes, draw 0.5mL after abundant mixing and add No. 3, the like until be diluted to No. 9 pipes.No. 10 do not add extract in contrast.Each pipe cultivates 24h at putting into incubator 37 DEG C after adding 0.5mL bacterium liquid for subsequent use, observes and records result (the results are shown in Table 3).
Table 3 each extract minimal inhibitory concentration measurement result
As seen from the above table, compound recipe Herba polygoni hydropiperis decocting ethanol extract all has good fungistatic effect to S. pullonum, chicken colibacillosis and staphylococcus aureus, MIC is 15.625mg/mL, in addition compound recipe Herba polygoni hydropiperis decocting ethanol extract is compared to other extracts, also have higher antibacterial performance for Candida albicans and bacillus pyocyaneus, MIC is 31.25mg/mL.
Result of the test shows: compound recipe Herba polygoni hydropiperis decocting ethanol extract all has stronger extracorporeal extracorporeal suppression to S. pullonum and chicken colibacillosis, and this kind of clinical conventional antibiotic of penicillin may because the generation of drug resistance can not play fungistatic effect.Further, compound recipe Herba polygoni hydropiperis decocting ethanol extract all shows In Vitro Bacteriostasis effect in various degree to staphylococcus aureus and bacillus pyocyaneus.In addition, this poultry husbandry Common Pathogenic fungus of Candida albicans also presents sensitivity to compound recipe Herba polygoni hydropiperis decocting ethanol extract.
In Example 1, other formula repeat above-mentioned test, and result is similar, do not repeat them here.
As fully visible, the Chinese herbal medicine extract with antibacterial action of the present invention all shows good In Vitro Bacteriostatic to the common 5 kinds of diarrhea inducing bacterium of poultry husbandry, has broad spectrum activity.
Claims (3)
1. there is a Chinese herbal medicine extract for antibacterial action, it is characterized in that being made up through decocting alcohol extraction of the raw medicinal material of following weight portion: Herba polygoni hydropiperis 120 ~ 180 parts, Herba Pogostemonis 120 ~ 180 parts, Fructus Ligustri Lucidi 120 ~ 180 parts, the Radix Astragali 80 ~ 120 parts.
2. there is described in claim 1 preparation method of the Chinese herbal medicine extract of antibacterial action, it is characterized in that making after each raw medicinal material mixing through decocting alcohol extraction.
3. the preparation method with the Chinese herbal medicine extract of antibacterial action according to claim 2, is characterized in that described decocting alcohol extraction is undertaken by following operation: take each raw medicinal material 20g altogether by weight, pulverize, mixing, add 10 times of soak by water 3 times, water temperature 100 DEG C, each 1.5 hours; Filter, after merging 3 filtrates, the centrifugal 30min of 3000r/min collects supernatant; Adding 95% ethanol after supernatant Rotary Evaporators is concentrated makes ethanol content be 80%, filters and remove precipitation after leaving standstill 24h; Filtrate, to collect supernatant after the centrifugal 30min of 3000r/min, reclaims ethanol with Rotary Evaporators.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105663359A (en) * | 2016-02-23 | 2016-06-15 | 四川农业大学 | Traditional Chinese medicine composition used for bacteriostasis in vitro of blue-shell chickens and preparation method thereof |
| CN107582644A (en) * | 2017-08-23 | 2018-01-16 | 陈居旭 | A kind of Chinese medicine composition with detoxify clear dysentery and anti-diarrhea effect |
| CN107653675A (en) * | 2017-11-13 | 2018-02-02 | 湖州佳伟纺织品有限公司 | Textiles antibacterial finishing agent and preparation method thereof |
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| CN1513508A (en) * | 2003-06-03 | 2004-07-21 | 金陵药业股份有限公司技术中心 | Medicine composition for treating uppe respiration channel infetion and its preparing process |
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| CN1513508A (en) * | 2003-06-03 | 2004-07-21 | 金陵药业股份有限公司技术中心 | Medicine composition for treating uppe respiration channel infetion and its preparing process |
Non-Patent Citations (2)
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| 刘晓蓉等: "广藿香提取物的抑菌活性研究", 《食品科技》 * |
| 缪珊等: "辣蓼的抑菌抗炎实验研究", 《中国新医药》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105663359A (en) * | 2016-02-23 | 2016-06-15 | 四川农业大学 | Traditional Chinese medicine composition used for bacteriostasis in vitro of blue-shell chickens and preparation method thereof |
| CN105663359B (en) * | 2016-02-23 | 2019-10-18 | 四川农业大学 | A kind of Chinese medicine composition and preparation method thereof for layer of green-shell egg In Vitro Bacteriostasis |
| CN107582644A (en) * | 2017-08-23 | 2018-01-16 | 陈居旭 | A kind of Chinese medicine composition with detoxify clear dysentery and anti-diarrhea effect |
| CN107653675A (en) * | 2017-11-13 | 2018-02-02 | 湖州佳伟纺织品有限公司 | Textiles antibacterial finishing agent and preparation method thereof |
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