CN105055539B

CN105055539B - Chinese herbal medicine extract and preparation method thereof with antibacterial action

Info

Publication number: CN105055539B
Application number: CN201510573480.5A
Authority: CN
Inventors: 胡庭俊; 陶俊宇; 谭红连; 李璐; 韦英益; 曾芸
Original assignee: Guangxi University
Current assignee: Guangxi University
Priority date: 2015-09-10
Filing date: 2015-09-10
Publication date: 2019-01-29
Anticipated expiration: 2035-09-10
Also published as: CN105055539A

Abstract

The invention discloses a kind of Chinese herbal medicine extracts with antibacterial action, are made of the raw medicinal material of following parts by weight through decocting alcohol extracting: 120~180 parts of polygonum flaccidum, 120~180 parts of Pogostemon cablin, 120~180 parts of the fruit of glossy privet, 80~120 parts of Radix Astragali.Extracorporeal bacteria inhibitor test the result shows that, Chinese herbal medicine extract of the invention has good antibacterial effect, has broad spectrum activity, and its antibacterial effect is more preferable compared with water extract, Microwave Extraction object, alcohol grading extract.Antibacterials by the present invention as birds substitute antibiotics and chemicals can effectively avoid the pollution of drug resistance and medicament residue to livestock product, reduces poultry nocuousness excreta, enzyme protein dosage and polluted by nitrogen, has a vast market foreground.

Description

Chinese herbal medicine extract and preparation method thereof with antibacterial action

Technical field

The invention belongs to antibacterials technical field more particularly to a kind of Chinese herbal medicine extract with antibacterial action and its Preparation method.

Background technique

With the fast development of aquaculture, antibiotic is using more and more extensive, in terms of Animal diseases Play certain positive effect.But in recent years, drug resistance problems caused by abuse of the antibiotic in aquaculture Through can not be ignored.Moreover, long-term a large amount of not scientifical use antibiotic can also make animal wastes containing heavy-metal residual, cause environment dirty Dye, brings serious harm to human health, and the rapid development of aquaculture not only increases the supply of animal product, while also band The problem of having carried out food safety.Wide spectrum, low toxicity, noresidue Chinese medicine compound prescription in veterinary clinic using more and more extensive, increase Strong immunity, promotes the favor that aquaculture is increasingly obtained the characteristics of growth at broad-spectrum antiseptic.Therefore, it researches and develops a kind of with antibacterial Effect, nontoxic residue-free, alternative antibiotic herbal product be the inexorable trend of modern farming and social development, have Significance and application value.

Summary of the invention

The technical problem to be solved in the present invention is to provide a kind of has a broad antifungal spectrum, nontoxic residue-free have antibacterial action in Herb extracts and preparation method thereof.

In order to solve the above technical problems, the invention adopts the following technical scheme: with antibacterial action Chinese herbal medicine extract, It is made of the raw medicinal material of following parts by weight through decocting alcohol extracting: 120~180 parts of polygonum flaccidum, 120~180 parts of Pogostemon cablin, the fruit of glossy privet 120~180 parts, 80~120 parts of Radix Astragali.

The preparation method of the above-mentioned Chinese herbal medicine extract with antibacterial action, through decocting alcohol extracting after each raw medicinal material is mixed It is made.

Decocting alcohol extracting is carried out by following operation: being weighed the total 20g of each raw medicinal material by weight, is crushed, mixing adds 10 times of water It decocts 3 times, 100 DEG C of water temperature, every time 1.5 hours；Filtering, 3000r/min centrifugation 30min collects supernatant after merging 3 filtrates； 95% ethyl alcohol is added after supernatant is concentrated into small size with Rotary Evaporators makes ethanol content be 80%, stands for 24 hours mistake afterwards It filters off except precipitating；Filtrate collects supernatant after being centrifuged 30min with 3000r/min, ethyl alcohol is recycled with Rotary Evaporators, by gained medicine Liquid is settled to after 20mL and saves backup for 4 DEG C.

Aiming at the problem that drug abuse in current aquaculture, inventor is guidance with traditional Chinese veterinarian's theory, and prescription is prepared A kind of compound polygonum flaccidum dissipates, the raw medicinal material including following parts by weight: 120~180 parts of polygonum flaccidum, 120~180 parts of Pogostemon cablin, and female 80~120 parts of Radix Astragali, one kind has been made with anti-through decocting alcohol extracting by the raw medicinal material of the above parts by weight in loyal 120~180 parts of son The Chinese herbal medicine extract of bacterium effect.Extracorporeal bacteria inhibitor test the result shows that, Chinese herbal medicine extract of the invention have good antibacterial Effect has broad spectrum activity, and its antibacterial effect is more preferable compared with water extract, Microwave Extraction object, alcohol grading extract.It incite somebody to action this The antibacterials as birds substitute antibiotics and chemicals are invented, drug resistance and medicament residue is can effectively avoid and poultry is produced The pollution of product reduces poultry nocuousness excreta, enzyme protein dosage and polluted by nitrogen, has a vast market foreground.

Specific embodiment

Embodiment 1 has the preparation of the Chinese herbal medicine extract of antibacterial action

Formula: 120~180 parts of polygonum flaccidum, 120~180 parts of Pogostemon cablin, 120~180 parts of the fruit of glossy privet, 80~120 parts of Radix Astragali. Each sample specific formula is shown in Table 1.

Table 1 has the formula table of the Chinese herbal medicine extract of antibacterial action

Sample formulations serial number	Polygonum flaccidum	Pogostemon cablin	The fruit of glossy privet	Radix Astragali
					1	120 parts	145 parts	180 parts	80 parts
2	180 parts	120 parts	125 parts	120 parts
					3	150 parts	180 parts	165 parts	95 parts
4	120 parts	120 parts	120 parts	80 parts

Preparation method: being made after each raw medicinal material is mixed through decocting alcohol extracting, and decocting alcohol extracting is carried out by following operation: by weight The total 20g of each raw medicinal material is weighed, is crushed, mixing adds 10 times of water to decoct 3 times, 100 DEG C of water temperature, every time 1.5 hours；Filtering merges 3000r/min is centrifuged 30min and collects supernatant after 3 filtrates；It is added after supernatant is concentrated into small size with Rotary Evaporators 95% ethyl alcohol makes ethanol content be 80%, stands for 24 hours filtering removal precipitating afterwards；Filtrate is received after being centrifuged 30min with 3000r/min Collect supernatant, recycle ethyl alcohol with Rotary Evaporators, gained medical fluid is settled to after 20mL and is saved backup for 4 DEG C.

The preparation of 2 control sample of embodiment

Water extract control

The total 20g of each raw material is weighed by formula 4, is crushed, mixing adds 10 times of water to decoct 3 times, and 100 DEG C of water temperature, 1.5 is small every time When；Filtering, after merging 3 filtrates, 3000r/min is centrifuged 30min and collects supernatant, and supernatant Rotary Evaporators are concentrated simultaneously It is settled to after 20ml and saves backup for 4 DEG C.

The control of Microwave Extraction object

The total 20g of each raw material is weighed by formula 4, is crushed, mixing after adding 10 times of water to be sufficiently mixed, is placed in microwave sample processing 100 DEG C of processing 15min of system.

The control of alcohol grading extract

The total 20g of each raw material is weighed by formula 4, is crushed, mixing is separately added into 95%, 70%, 45% ethyl alcohol of 10 times of volumes Extraction 3 times, extraction time 48h, for 24 hours, for 24 hours.

Chinese herbal medicine extract of the embodiment 3 with antibacterial action applies pressure testing

1. test material

The sample (hereinafter referred to as compound polygonum flaccidum decocting alcohol extract or decocting alcohol extract) of formula 4 and implementation in Example 1 Control sample in example 2 is used for following tests.

2. In Vitro Bacteriostasis effect measuring

The preparation of bacterium solution: by S. pullonum, chicken colibacillosis, staphylococcus aureus, Candida albicans and The test bacterias such as Pseudomonas aeruginosa are inoculated in nutrient agar panel after sufficiently recovering, cultivate at 37 DEG C for 24 hours, the inoculation of picking colonies typical In nutrient broth medium, 18h is cultivated at 37 DEG C, bacterial concentration is tuned into 3 × 10⁸A/mL is spare.

Measuring method: measuring the In Vitro Bacteriostasis effect of each extract with paper disk method, using penicillin, streptomysin as control. Tested bacterium solution is spread evenly across on the nutrient agar panel in the area Fen Hao, slightly sets a moment, will sufficiently draw the paper of each tested medical fluid Piece is affixed on region center, is buckled to plate, cultivates at 37 DEG C and measures antibacterial circle diameter and recording after 12h~18h and (the results are shown in Table 2)。

Antibacterial intensity criterion: no inhibition zone is judged to insensitive；Antibacterial circle diameter is less than 10mm person and is judged to less sensitive； Person is judged to medium sensitivity between 10~15mm；Person is judged to highly sensitive between 16~20mm；20mm or more person is judged to extremely sensitive.

Each extract In Vitro Bacteriostasis circle measurement result of table 2

As seen from the above table, S. pullonum is highly sensitive to compound polygonum flaccidum decocting alcohol extract；Chicken colibacillosis is to multiple Square polygonum flaccidum decocting alcohol extract is highly sensitive；Staphylococcus aureus is highly sensitive to compound polygonum flaccidum decocting alcohol extract；Candida albicans Bacterium is to compound polygonum flaccidum decocting alcohol extract less sensitive；Pseudomonas aeruginosa is to compound polygonum flaccidum decocting alcohol extract less sensitive.

3. the measurement of minimal inhibitory concentration

Above-mentioned each extract is measured to S. pullonum, chicken colibacillosis, Staphylococcus aureus with test tube dichotomy The minimal inhibitory concentration MIC of bacterium, Candida albicans and Pseudomonas aeruginosa.0.5mL nutrition is added into 1 to No. 10 EP pipe for sterile working Meat soup draws each extract of 0.5mL and No. 1 pipe is added, mixes well, and 0.5mL liquid is drawn from No. 1 pipe, No. 2 pipes are added, sufficiently Draw 0.5mL after mixing and be added No. 3, and so on until be diluted to No. 9 pipes.No. 10 are used as control that extract is not added.Each pipe adds Enter to be put at 37 DEG C of incubator after the spare bacterium solution of 0.5mL and cultivate for 24 hours, observes and records result (the results are shown in Table 3).

Each extract minimal inhibitory concentration measurement result of table 3

As seen from the above table, compound polygonum flaccidum decocting alcohol extract is to S. pullonum, chicken colibacillosis and golden yellow Portugal Grape coccus has preferable fungistatic effect, and MIC is 15.625mg/mL, and furthermore compound polygonum flaccidum decocting alcohol extract is compared to other Extract also has higher antibacterial performance for Candida albicans and Pseudomonas aeruginosa, and MIC is 31.25mg/mL.

Test result shows: compound polygonum flaccidum decocting alcohol extract has S. pullonum with chicken colibacillosis stronger Extracorporeal extracorporeal suppression, and to may be because that the generation of drug resistance cannot have been played antibacterial for this kind of clinically used antibiotic of penicillin Effect.Also, compound polygonum flaccidum decocting alcohol extract shows different degrees of external suppression to staphylococcus aureus and Pseudomonas aeruginosa Bacterium effect.In addition, sensitivity is also presented to compound polygonum flaccidum decocting alcohol extract in this poultry husbandry Common Pathogenic fungi of Candida albicans.

Other formulas repeat above-mentioned test in Example 1, and as a result similar, details are not described herein.

To sum up, bacterium all tables are led to diarrhea for 5 kinds common to poultry husbandry of the Chinese herbal medicine extract with antibacterial action of the invention Existing good In Vitro Bacteriostatic, has broad spectrum activity.

Claims

1. a kind of Chinese herbal medicine extract with antibacterial action, it is characterised in that by the raw medicinal material of following parts by weight through decocting alcohol It obtains through refining into: 120~180 parts of polygonum flaccidum, 120~180 parts of Pogostemon cablin, 120~180 parts of the fruit of glossy privet, 80~120 parts of Radix Astragali；The water It decocts alcohol extracting to carry out by following operation: weighing the total 20g of each raw medicinal material by weight, crush, mixing adds 10 times of water to decoct 3 times, water 100 DEG C, every time 1.5 hours of temperature；Filtering, 3000r/min centrifugation 30min collects supernatant after merging 3 filtrates；Supernatant is used 95% ethyl alcohol is added after Rotary Evaporators concentration makes ethanol content be 80%, stands for 24 hours filtering removal precipitating afterwards；Filtrate with Supernatant is collected after 3000r/min centrifugation 30min, recycles ethyl alcohol with Rotary Evaporators.