CN102125062A - Compound lightyellow sophora root preparation and application thereof - Google Patents
Compound lightyellow sophora root preparation and application thereof Download PDFInfo
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- CN102125062A CN102125062A CN2011100820280A CN201110082028A CN102125062A CN 102125062 A CN102125062 A CN 102125062A CN 2011100820280 A CN2011100820280 A CN 2011100820280A CN 201110082028 A CN201110082028 A CN 201110082028A CN 102125062 A CN102125062 A CN 102125062A
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Abstract
The invention belongs to the technical field of research and development of medicinal products and relates to a compound lightyellow sophora root preparation and application thereof. The compound lightyellow sophora root preparation is prepared by the following steps of: adding ethanol into lightyellow sophora root, melia azedarach linn leaf and salix leaf respectively; oscillating and lixiviating by using a table concentrator; centrifuging by using a centrifugal machine to obtain supernate; extracting repeatedly for twice; combining the supernate of the three times; filtering; concentrating the filtrate under reduced pressure; drying to obtain extracts of the lightyellow sophora root, the melia azedarach linn leaf and the salix leaf; adding pounded garlic into a round-bottomed flask to perform steam distillation to obtain garlic essential oil; and preparing the compound lightyellow sophora root preparation from the lightyellow sophora root extract, the melia azedarach linn leaf extract, the salix leaf extract and the garlic essential oil. The compound lightyellow sophora root preparation has bacteriostasis on vibrio anguillarum, vibro harveyi, vibrio alginnolyficus and halophilic vibrio. The corresponding preparation can be developed to be used for preventing and treating vibriosis in the aquaculture process. The compound lightyellow sophora root preparation is suitable for industrialized production.
Description
Technical field
Medical product research and development technical field under the present invention.The present invention relates to a kind of Compound Kushen ' and uses thereof.Product of the present invention has bacteriostasis to D31 (Vibrio anguillarum V.anuguillarum), D32 (Vibrio harveyi V.harveyi), D61 (vibrio alginolyticus V.alginolyticus), D230 (halophilic vibrio Vibrio vulnificus), can develop corresponding preparations and be used for preventing and treating the vibriosis that the aquaculture process takes place, product of the present invention is fit to suitability for industrialized production.
Background technology
China greatly develops mariculture industry in recent years, but because of cultured area overrun expansion, cultivation density is too high and miscarriage, the numerous life of cultured fishes disease has caused enormous economic loss.This is one of the severe diseases the most that occurs in the putative fish culture process by the vibriosis that D31 (Vibrio anguillarum V.anuguillarum), D32 (Vibrio harveyi V.harveyi), D61 (vibrio alginolyticus V.alginolyticus), D230 (Vibrio vulnificus) cause wherein, it also is the importance factor that limits this industry development, vibrios can also cause food poisoning by food chain in addition, and the harm eater's is healthy.Yet, at present China mainly based on chemicalses such as antibiotic, but because long-term abuse has caused a series of problems such as serious environmental pollution, the anti-medicine of germ, person poultry safety are on the hazard for the disease prevention and cure of aquiculture animal.
The chemicals of comparing, plant resource antibacterial medicines have that biocidal efficacies is stronger, and toxicity is lower, be difficult for developing immunity to drugs, and advantage such as with low cost, therefore seeking the bacteriostatic activity material from plant is an effective way that ensures the stable development of fish culture industry.But be engaged in recent years regrettably and cause the work of fish disease bacterium bacteriostatic activity foliage filter screening and but deviate from mutually with its advantage, the potent bactericidal activity of pursuit simply, make screened plant gather in the expensive class plant of high poison, this makes the exploitation of botanical fungicide popularize stagnation, inquires into therefore that exploitation is efficient, low toxicity, inexpensive botanical fungicide be very urgent.
Kuh-seng is the root of leguminous plant kuh-seng Sophora flavescens.In vitro test proves, 1% matrine all has obvious inhibitory action to shigella dysenteriae, Escherichia coli, proteus, beta streptococcus and staphylococcus aureus. and pure medicinal extract still has an anti-trichomonal effect external, intensity is weaker than the coptis, and it is close with frutus cnidii. in test tube, kuh-seng decoction high concentration (1: 100) has inhibitory action to tubercle bacillus. and decoction (8%), water logging agent (1: 3) have in various degree inhibitory action external to some common skin fungi.
Folium Meliae is the dried leaves of Meliaceae deciduous tree plant China tree Melia azedarach Linn..Folium Meliae has heat-clearing and damp-drying drug, killing parasites to relieve itshing, promoting qi circulation and relieving pain effect.
Willow leaf is the dried leaves of Salicaceae plant weeping willow Salix babylonica L.Willow leaf has heat-clearing, promoting eruption, and diuresis, the effect of detoxifcation for oral administration is used for the treatment of various inflammation infections such as the infection of the upper respiratory tract, tracheitis, pneumonia, cystitis, sphagitis, is applied to diseases such as furuncle, pyogenic parotitis, mastitis outward.
Galic essential oil is the volatile oil that the bulb of garlic Allium sativum L. (Garlic) obtains through steam distillation.It is about 0.2% that garlic contains volatile oil, and main component is an allicin in the oil, has bactericidal action, is that alliin contained in the garlic is produced by the effect hydrolysis of big allinase.
Summary of the invention
1, a kind of Compound Kushen ' is characterized in that it being the preparation of being made by the following weight proportioning:
Shrubby sophora extract 2g-3g, Folium Meliae extract 1g-2g, willow leaf extract 1g-2g, galic essential oil 0.001g-0.002g.
2, the preparation method of the described a kind of Compound Kushen ' of claim 1 is characterized in that may further comprise the steps:
Step (one): get each 30g-40g of kuh-seng, Folium Meliae and willow leaf respectively, add 600mL-800mL ethanol respectively, with shaking table concussion lixiviate 24h, with centrifugal supernatant and the residue of getting of centrifuge;
Step (two): residue in () is added 400mL-600mL ethanol respectively, with shaking table concussion lixiviate 18h, centrifugal supernatant and the residue of getting of centrifuge;
Step (three): residue in (two) is added 200mL-400mL ethanol respectively, concussion lixiviate 12h, centrifugal supernatant and the residue of getting of centrifuge, residue discards, merge three times supernatant, filter, get filtrate, filtrate is carried out concentrating under reduced pressure, and oven dry obtains kuh-seng, Folium Meliae and willow leaf extract;
Step (four): get the garlic 10g-20g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 1.5h-2.0h leaves standstill, and gets galic essential oil;
Step (five): make Compound Kushen ' by the described weight proportion of claim 1.
3, the purposes of the described a kind of Compound Kushen ' of claim 1 is characterized in that Vibrio anguillarum, Vibrio harveyi, vibrio alginolyticus, halophilic vibrio are had bacteriostasis.
According to the present invention, " % " among the present invention is percentage by weight.
Embodiment
A kind of Compound Kushen ' and uses thereof that relates to of the present invention comprises following examples, and the following examples can further specify the present invention, but do not limit the present invention in any way.
Embodiment 1:
1, a kind of Compound Kushen ' is characterized in that it being the preparation of being made by the following weight proportioning:
Shrubby sophora extract 2g, Folium Meliae extract 1g, willow leaf extract 1g, galic essential oil 0.001g.
2, the preparation method of the described a kind of Compound Kushen ' of claim 1 is characterized in that may further comprise the steps:
Step (one): get each 30g of kuh-seng, Folium Meliae and willow leaf respectively, add 600mL ethanol respectively, with shaking table concussion lixiviate 24h, with centrifugal supernatant and the residue of getting of centrifuge;
Step (two): residue in () is added 400mL ethanol respectively, with shaking table concussion lixiviate 18h, centrifugal supernatant and the residue of getting of centrifuge;
Step (three): residue in (two) is added 200mL ethanol respectively, concussion lixiviate 12h, centrifugal supernatant and the residue of getting of centrifuge, residue discards, merge three times supernatant, filter, get filtrate, filtrate is carried out concentrating under reduced pressure, and oven dry obtains kuh-seng, Folium Meliae and willow leaf extract;
Step (four): get the garlic 10g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 1.5h leaves standstill, and gets galic essential oil;
Step (five): make Compound Kushen ' by the described weight proportion of claim 1.
3, the purposes of the described a kind of Compound Kushen ' of claim 1 is characterized in that Vibrio anguillarum, Vibrio harveyi, vibrio alginolyticus, halophilic vibrio are had bacteriostasis.
Embodiment 2:
1, a kind of Compound Kushen ' is characterized in that it being the preparation of being made by the following weight proportioning:
Shrubby sophora extract 3g, Folium Meliae extract 2g, willow leaf extract 2g, galic essential oil 0.002g.
2, the preparation method of the described a kind of Compound Kushen ' of claim 1 is characterized in that may further comprise the steps:
Step (one): get each 40g of kuh-seng, Folium Meliae and willow leaf respectively, add 800mL ethanol respectively, with shaking table concussion lixiviate 24h, with centrifugal supernatant and the residue of getting of centrifuge;
Step (two): residue in () is added 600mL ethanol respectively, with shaking table concussion lixiviate 18h, centrifugal supernatant and the residue of getting of centrifuge;
Step (three): residue in (two) is added 400mL ethanol respectively, concussion lixiviate 12h, centrifugal supernatant and the residue of getting of centrifuge, residue discards, merge three times supernatant, filter, get filtrate, filtrate is carried out concentrating under reduced pressure, and oven dry obtains kuh-seng, Folium Meliae and willow leaf extract;
Step (four): get the garlic 20g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 2.0h leaves standstill, and gets galic essential oil;
Step (five): make Compound Kushen ' by the described weight proportion of claim 1.
3, the purposes of the described a kind of Compound Kushen ' of claim 1 is characterized in that Vibrio anguillarum, Vibrio harveyi, vibrio alginolyticus, halophilic vibrio are had bacteriostasis.
Pharmacological evaluation
The bacteriostasis experiment of Compound Kushen '
1 material and method
1.1 for the examination material
1.1.1 strains tested
This is tested employed four and causes the fish disease bacterium all from Microbiological Lab of Shandong University ai Weihai, is respectively D31 (Vibrio anguillarum V.anuguillarum), D32 (Vibrio harveyi V.harveyi), D61 (vibrio alginolyticus V.alginolyticus), D230 (halophilic vibrio Vibriovulnificus).Germ is cultivated with 2216E solid and liquid nutrient medium in the test.
The 2216E medium: peptone 5g, yeast extract 1g, high ferric phosphate 0.01g, Chen Haishui 1000mL, 5% sodium hydroxide solution that boils is transferred pH value 7.6-7.8, agar 15-20g (liquid nutrient medium does not add).
1.1.2 test apparatus
Superclean bench, illumination box, electronic cursor slide calliper rule, centrifuge, Rotary Evaporators, baking oven, medicinal herb grinder, high-pressure sterilizing pot etc.
1.1.3 test plant sample
It is kuh-seng, Folium Meliae, willow leaf, garlic that plant is chosen in this experiment.
1.2 experimental technique
1.2.1 the preparation of plant extracts and the preparation of Compound Kushen '
Get kuh-seng, Folium Meliae, each 40g of willow leaf respectively, add 800mL mL ethanol respectively, with shaking table concussion lixiviate 24h, with centrifugal supernatant (1) and the residue (1) of getting of centrifuge; Residue (1) is added 600mL ethanol respectively, with shaking table concussion lixiviate 18h, centrifugal supernatant (2) and the residue (2) of getting of centrifuge; Residue (2) is added 400mL ethanol respectively, concussion lixiviate 12h, centrifugal supernatant (3) and the residue (3) of getting of centrifuge, residue discards, merge three times supernatant, filter, get filtrate, filtrate is carried out concentrating under reduced pressure, and oven dry obtains kuh-seng, Folium Meliae, willow leaf extract; Get the garlic 20g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 2.0h leaves standstill, and gets galic essential oil;
Get shrubby sophora extract 3g, Folium Meliae extract 2g, willow leaf extract 2g and galic essential oil 0.002g preparation compound polygonum flaccidum preparation.Reclaim ethanol as the dissolution with solvents Compound Kushen ' to revolve to steam, making crude drug content is 1g/mL, and 0～4 ℃ of refrigerator in sealing back is preserved stand-by.
1.2.2 Compound Kushen ' is to causing the mensuration of fish disease vibrios bacteriostatic activity
1.2.2.1 the preparation of bacteria suspension
Transfer after bacterial strain is activated in solid plate in 28 ℃ cultivate 24h after, provoke an amount of lawn and be inoculated in liquid nutrient medium, then again in the shaken cultivation case 28 ℃ cultivate 24h, all muddy back of each conical flask spread plate counting also is diluted to 4 * 10
8CFU/mL is standby.
1.2.2.2 Compound Kushen ' is to causing the test of fish disease vibrios bacteriostatic activity
Adopt dull and stereotyped diffusion process, the preparation diameter is the 2216E solid culture medium flat board of 10cm, requires each slab-thickness 4mm, add each bacteria suspension coating of 100 μ l and evenly place 30min in the back, dull and stereotyped bottom setting-out Fen San district, each makes a call to a hole to distinguish central card punch in each, and the card punch diameter is 5mm; Test is with the positive contrast of carbendazim, and carbendazim concentration is 1mg/mL, steam to reclaim ethanol as solvent control to revolve, every kind of bacterium do three parallel, drip Compound Kushen ' 30 μ L in each hole, finish the back and place 30min.After placement finishes flat board placed in 28 ℃ of incubators and cultivate 24h, the vernier caliper measurement antibacterial circle diameter.
The judgement of fungistatic effect is with reference to list of references, and antibacterial circle diameter＞20mm represents with " * * * " for extremely quick; 15-19mm is Gao Min, represents with " * * "; 10-14 is quick in being, represents with " * "; Be muting sensitive below 10, represent with "-".
2 results
2.1 Compound Kushen ' is to causing the inhibitory action of fish disease vibrios
See Table 1.
Table 1 Compound Kushen ' is to causing the inhibition activity of fish disease vibrios
Annotate: " A " represents antibacterial circle diameter/mm; " B " represents relative sensitivity.
Claims (3)
1. Compound Kushen ' is characterized in that it being the preparation of being made by the following weight proportioning:
Shrubby sophora extract 2g-3g, Folium Meliae extract 1g-2g, willow leaf extract 1g-2g, galic essential oil 0.001g-0.002g.
2. the preparation method of the described a kind of Compound Kushen ' of claim 1 is characterized in that may further comprise the steps:
Step (one): get each 30g-40g of kuh-seng, Folium Meliae and willow leaf respectively, add 600mL-800mL ethanol respectively, with shaking table concussion lixiviate 24h, with centrifugal supernatant and the residue of getting of centrifuge;
Step (two): residue in () is added 400mL-600mL ethanol respectively, with shaking table concussion lixiviate 18h, centrifugal supernatant and the residue of getting of centrifuge;
Step (three): residue in (two) is added 200mL-400mL ethanol respectively, concussion lixiviate 12h, centrifugal supernatant and the residue of getting of centrifuge, residue discards, merge three times supernatant, filter, get filtrate, filtrate is carried out concentrating under reduced pressure, and oven dry obtains kuh-seng, Folium Meliae and willow leaf extract;
Step (four): get the garlic 10g-20g that smashs to pieces, add in the 1000mL round-bottomed flask, carry out steam distillation, distillation time 1.5h-2.0h leaves standstill, and gets galic essential oil;
Step (five): make Compound Kushen ' by the described weight proportion of claim 1.
3. the purposes of the described a kind of Compound Kushen ' of claim 1 is characterized in that Vibrio anguillarum, Vibrio harveyi, vibrio alginolyticus, halophilic vibrio are had bacteriostasis.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110082028 CN102125062B (en) | 2011-03-28 | 2011-03-28 | Compound lightyellow sophora root preparation and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110082028 CN102125062B (en) | 2011-03-28 | 2011-03-28 | Compound lightyellow sophora root preparation and application thereof |
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| CN102125062A true CN102125062A (en) | 2011-07-20 |
| CN102125062B CN102125062B (en) | 2013-06-05 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103463563A (en) * | 2013-08-30 | 2013-12-25 | 兴化市恒威生物技术有限公司 | Medicinal composition for treating side rot disease of sea cucumber and preparation method thereof |
| CN104094979A (en) * | 2014-07-04 | 2014-10-15 | 李钟� | Willow leaf for preparing sanitizer |
| CN104886180A (en) * | 2015-06-02 | 2015-09-09 | 张亚南 | Plant source pesticide used for planting fruits and vegetables |
| CN107149050A (en) * | 2017-06-21 | 2017-09-12 | 沭阳益客食品有限公司 | Chinese herbal feed additive for laying hen health care and preparation method thereof |
| CN113499337A (en) * | 2021-08-11 | 2021-10-15 | 江苏海洋大学 | Application of kurarinone in preparation of aquatic pathogen resisting medicine or feed additive |
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| CN1199550A (en) * | 1997-05-21 | 1998-11-25 | 刘军 | Compound chinaberry-Radix Sophorae Flavescentis pesticide for animal and plant protection |
| CN1316266A (en) * | 2001-02-12 | 2001-10-10 | 高福兴 | Process for extracting natural medicine for pine, cypress and fir |
| CN1421140A (en) * | 2002-12-12 | 2003-06-04 | 张恒山 | Pesticidal matrine solution and its production process |
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2011
- 2011-03-28 CN CN 201110082028 patent/CN102125062B/en not_active Expired - Fee Related
Patent Citations (3)
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| CN1199550A (en) * | 1997-05-21 | 1998-11-25 | 刘军 | Compound chinaberry-Radix Sophorae Flavescentis pesticide for animal and plant protection |
| CN1316266A (en) * | 2001-02-12 | 2001-10-10 | 高福兴 | Process for extracting natural medicine for pine, cypress and fir |
| CN1421140A (en) * | 2002-12-12 | 2003-06-04 | 张恒山 | Pesticidal matrine solution and its production process |
Non-Patent Citations (2)
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| 柳富荣: "浅谈水产禁用药物及相应技术措施", 《内陆水产》 * |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103463563A (en) * | 2013-08-30 | 2013-12-25 | 兴化市恒威生物技术有限公司 | Medicinal composition for treating side rot disease of sea cucumber and preparation method thereof |
| CN103463563B (en) * | 2013-08-30 | 2015-05-20 | 兴化市恒威生物技术有限公司 | Medicinal composition for treating side rot disease of sea cucumber and preparation method thereof |
| CN104094979A (en) * | 2014-07-04 | 2014-10-15 | 李钟� | Willow leaf for preparing sanitizer |
| CN104094979B (en) * | 2014-07-04 | 2017-04-05 | 李钟� | Application of the Folium Salicis Babylonicae in disinfectant is prepared |
| CN104886180A (en) * | 2015-06-02 | 2015-09-09 | 张亚南 | Plant source pesticide used for planting fruits and vegetables |
| CN107149050A (en) * | 2017-06-21 | 2017-09-12 | 沭阳益客食品有限公司 | Chinese herbal feed additive for laying hen health care and preparation method thereof |
| CN113499337A (en) * | 2021-08-11 | 2021-10-15 | 江苏海洋大学 | Application of kurarinone in preparation of aquatic pathogen resisting medicine or feed additive |
| CN113499337B (en) * | 2021-08-11 | 2023-04-28 | 江苏海洋大学 | Application of kurarinone in preparation of aquatic pathogenic bacteria resistant medicines or feed additives |
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| CN102125062B (en) | 2013-06-05 |
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