CN105053768A - Preparation method of active pollen nutrient solution - Google Patents
Preparation method of active pollen nutrient solution Download PDFInfo
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- CN105053768A CN105053768A CN201510404424.9A CN201510404424A CN105053768A CN 105053768 A CN105053768 A CN 105053768A CN 201510404424 A CN201510404424 A CN 201510404424A CN 105053768 A CN105053768 A CN 105053768A
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- 235000015097 nutrients Nutrition 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 27
- 230000035784 germination Effects 0.000 claims abstract description 41
- 238000000338 in vitro Methods 0.000 claims abstract description 27
- 229930006000 Sucrose Natural products 0.000 claims abstract description 19
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims abstract description 19
- 239000001963 growth medium Substances 0.000 claims description 29
- 238000000034 method Methods 0.000 claims description 20
- 239000005720 sucrose Substances 0.000 claims description 18
- 230000008569 process Effects 0.000 claims description 16
- 235000009917 Crataegus X brevipes Nutrition 0.000 claims description 13
- 235000013204 Crataegus X haemacarpa Nutrition 0.000 claims description 13
- 235000009685 Crataegus X maligna Nutrition 0.000 claims description 13
- 235000009444 Crataegus X rubrocarnea Nutrition 0.000 claims description 13
- 235000009486 Crataegus bullatus Nutrition 0.000 claims description 13
- 235000017181 Crataegus chrysocarpa Nutrition 0.000 claims description 13
- 235000009682 Crataegus limnophila Nutrition 0.000 claims description 13
- 235000004423 Crataegus monogyna Nutrition 0.000 claims description 13
- 235000002313 Crataegus paludosa Nutrition 0.000 claims description 13
- 235000009840 Crataegus x incaedua Nutrition 0.000 claims description 13
- 239000012530 fluid Substances 0.000 claims description 12
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 11
- 239000004327 boric acid Substances 0.000 claims description 11
- 241000234435 Lilium Species 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 239000012141 concentrate Substances 0.000 claims description 7
- 238000005286 illumination Methods 0.000 claims description 7
- 241001233914 Chelidonium majus Species 0.000 claims description 3
- 235000008585 Pinus thunbergii Nutrition 0.000 claims description 3
- 241000218686 Pinus thunbergii Species 0.000 claims description 3
- 229940068560 greater celandine Drugs 0.000 claims description 3
- -1 Compound amino acid Chemical class 0.000 claims description 2
- 240000000171 Crataegus monogyna Species 0.000 claims 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims 1
- 239000000126 substance Substances 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 235000005911 diet Nutrition 0.000 abstract 1
- 230000000378 dietary effect Effects 0.000 abstract 1
- 229960004793 sucrose Drugs 0.000 abstract 1
- 238000009210 therapy by ultrasound Methods 0.000 abstract 1
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- 235000016709 nutrition Nutrition 0.000 description 9
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- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 239000011149 active material Substances 0.000 description 2
- ZCCIPPOKBCJFDN-UHFFFAOYSA-N calcium nitrate Chemical compound [Ca+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ZCCIPPOKBCJFDN-UHFFFAOYSA-N 0.000 description 2
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- 235000012907 honey Nutrition 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
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- 239000002609 medium Substances 0.000 description 2
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- 238000001556 precipitation Methods 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 1
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
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- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052796 boron Inorganic materials 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
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- 230000036039 immunity Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
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- 230000000050 nutritive effect Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 238000010422 painting Methods 0.000 description 1
- 229920001277 pectin Polymers 0.000 description 1
- 235000010987 pectin Nutrition 0.000 description 1
- 239000001814 pectin Substances 0.000 description 1
- 238000000053 physical method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
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- 230000000717 retained effect Effects 0.000 description 1
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- 238000003786 synthesis reaction Methods 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a preparation method of an active pollen nutrient solution, and relates to the technical field of edible nutriments. The preparation method comprises the following steps: a) collecting edible pollen; b) making edible culture mediums according to the kinds of the collected pollen, wherein the culture mediums at least comprise cane sugar of which the weight percentage is 5-35%; c) performing in vitro germination on the pollen with the culture mediums so as to obtain a germination solution; and d) performing ultrasonic treatment on the germination solution so as to obtain the active pollen nutrient solution. The preparation method provided by the invention is simple in technology, the in vitro germination is performed on the pollen, so that various beneficial components in the pollen are released, the prepared pollen nutrient solution contains a large quantity of active components and nutrient substances, and the prepared pollen nutrient solution has a higher dietary value.
Description
Technical field
The present invention relates to edible nourishing product technical field, in particular to a kind of preparation method of active pollen nutrient solution.
Background technology
Pollen comes from the organ of multiplication of plant, wherein containing abundant nutritional labeling, as protein, auxin, enzyme, flavone compound, vitamin etc.In addition, pollen has multiple curative effect in health care, as improved body immunity, regulating human endocrine, skin care, prevent disease and person in middle and old age's common disease etc., can also resist various tumour simultaneously, resist radiation etc.The outer wall that pollen has one deck hard, the existence of this outer wall makes nutritional labeling in pollen substantially can not be absorbed by the body.Existing pollen wall breaking technology adopts physical technique fully can not abolish the outer wall of pollen, and chemical classes wall breaking technology then makes the nutritional labeling in pollen effectively not retained, thus greatly reduces nutrition and the use value of pollen.
Summary of the invention
The object of the present invention is to provide a kind of preparation method of active pollen nutrient solution, the nutriment in pollen can fully discharge by the method, and significantly improves the active component of nutriment in pollen, thus substantially increases pollen use value.
The present invention is achieved in that
A preparation method for active pollen nutrient solution, comprises the following steps:
A gathers edible pollen;
B makes edible culture medium according to the kind of the pollen gathering gained, and described culture medium comprises the sucrose that part by weight is 5% ~ 35%;
C utilizes described culture medium to carry out germination in vitro to pollen, to obtain germination fluid;
D carries out ultrasonic process to germination fluid, to obtain active pollen nutrient solution.
Pollen has higher nutritive value, effect, the nutritional labeling in pollen can be discharged, so that the absorption of human body and utilization by the mode of cultured in vitro.
Sucrose is that the sprouting of pollen provides abundant material base, can be pollen supply nutriment and carbon source, also play the effect of the osmotic pressure inside and outside the cell membrane regulating pollen simultaneously.If sucrose concentration is too low, then nutrients quality is inadequate, cannot provide sufficient energy, and then cause the germination rate of pollen to reduce to pollen.If the excessive concentration of sucrose, then may be that the pressure reduction change inside and outside pollen cell film is excessive, cell too much causes damage even dead due to dehydration.In addition, the osmotic potential of sucrose also adjustable pollen, makes it too absorb water and bursts, also can be used as cellular respiration substrate and and the raw material of synthetic starch, to promote the growth of pollen tube.
Boric acid then provides trace element for pollen, and can also regulate the acid-base value of pollen germination environment, for the sprouting of pollen provides micronutrient boron, the sprouting for pollen provides an adapt circumstance simultaneously.In addition, boric acid can promote that pollen is to the absorption of sugar and metabolism, improves the absorption to oxygen, participates in the synthesis of pectin substance.
As preferred scheme, the comprising of germination in vitro condition in step C: temperature 25 ~ 28 DEG C, time are 1 ~ 3 hour.
As preferred scheme, the germination in vitro condition in step C comprises: the intensity of illumination of 2000 ~ 3000Lux.
As preferred scheme, the pollen gathered in steps A is lilium pollen.
As preferred scheme, the culture medium in step B is composed as follows, wherein in part by weight:
As preferred scheme, the pollen gathered in steps A is Hawthorn Pollen.
As preferred scheme, the culture medium in step B is composed as follows, wherein in part by weight:
Sucrose 15%
Boric acid 0.1%
Surplus is water.
As preferred scheme, the pollen gathered in steps A is one or more in rape pollen, greater celandine pollen, Pollen In Pinus Thunbergii Parl., zasiokaurin.
As preferred scheme, the culture medium in step B is composed as follows, wherein in part by weight:
As preferred scheme, further comprising the steps of after step D:
Obtained active pollen nutrient solution carries out concentrated to remove moisture by E, and concentrated volume ratio is 40 ~ 60%, with obtained concentrate;
F concentrate carries out deepfreeze, and temperature is 0 ~ 4 DEG C.
Beneficial effect of the present invention:
Pollens nutrition liquid and preparation method thereof provided by the invention can significantly improve abolishes effect to pollen wall, and germination in vitro by pollen Middle nutrition substance release, can make the active material in pollen effectively be activated, can avoid the destruction to various beneficiating ingredient.In addition, the pollens nutrition liquid and preparation method thereof technique in the present invention is simple, it is low, consuming time short to consume energy, and reduces the preparation cost of painting style nutrient solution.
Accompanying drawing explanation
In order to be illustrated more clearly in the technical scheme of the embodiment of the present invention, be briefly described to the accompanying drawing used required in embodiment below, be to be understood that, the following drawings illustrate only some embodiment of the present invention, therefore the restriction to scope should be counted as, for those of ordinary skill in the art, under the prerequisite not paying creative work, other relevant accompanying drawings can also be obtained according to these accompanying drawings.
The flow chart of the preparation method of the active anthophorids honey that Fig. 1 provides for inventive embodiments 1;
The flow chart of the preparation method of the active anthophorids honey of another kind that Fig. 2 provides for inventive embodiments 1.
Detailed description of the invention
For making the object of the embodiment of the present invention, technical scheme and advantage clearly, below in conjunction with the accompanying drawing in the embodiment of the present invention, technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is the present invention's part embodiment, instead of whole embodiments.Below to the detailed description of the embodiments of the invention provided in the accompanying drawings and the claimed scope of the present invention of not intended to be limiting, but only represent selected embodiment of the present invention.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
Consult Fig. 1, Fig. 2, present embodiments provide a kind of preparation method of active pollen nutrient solution, comprise the following steps:
First, edible lilium pollen is gathered.
Pollen can adopt artificial collection, and the injury of artificial collection pollen to pollen is little, can show the situation causing pollen to sprout due to the mechanical damage of pollen.Optionally, gather pollen by pollen trap, pollen trap can improve the collecting efficiency of pollen, avoids pollen to be polluted.Pollen trap adopts commercial equipment, and the present embodiment does not do detailed description to its concrete structure.
Secondly, configuration pin is to the edible culture medium of lilium pollen, and culture medium at least comprises the sucrose that part by weight is 5% ~ 35%, in order to improve the boric acid that germination rate can add 0.1% ~ 0.2% in the medium.In the present embodiment, the base of cultivation is composed as follows, wherein in part by weight:
Again, by utilizing culture medium to carry out germination in vitro to pollen, to obtain germination fluid.
Germination in vitro preferably adopts constant-temperature shaking culture method.Conical flask is carried out clean, sterilization prevents the harmful substance in conical flask from affecting the smooth sprouting of lilium pollen.Pollen and culture medium are put into conical flask, and conical flask is positioned in constant temperature vibration incubator carries out cultured in vitro.Temperature in constant temperature vibration incubator is 26 DEG C, and incubation time is 1 hour, and the vibration frequency of conical flask is 100r/min.For the ease of observing the sprouting situation of lilium pollen, can the sprouting situation every half an hour to pollen observe and record.Such as, by microscopy, choose 5 ~ 6 visuals field, comprise in each visual field and be no less than 30 pollen, add up the sprouting situation of pollen by the way.When the germination rate of pollen is too low, can time of proper extension cultured in vitro.The germination rate of the present embodiment lilium pollen can reach 80%.
Finally, ultrasonic process is carried out to germination fluid, obtain active pollen nutrient solution.
After in-vitro pollen germination, the various active component wherein contained and beneficiating ingredient are activated, and then through sonic oscillation process 5 minutes, ultrasonic frequency is 50KHz, makes the precipitations such as the nutriment in pollen tube, thus obtain active pollen nutrient solution.According to the difference of the kind of pollen, the time of hyperacoustic selected frequency and process can adjust, and when the increase of the cell radius size of pollen, can improve hyperacoustic frequency and processing time, more thoroughly abolished with the pollen tube enable, thus release active ingredient wherein.
Preferably, in cultured in vitro process, in constant temperature vibration incubator, light irradiation apparatus is set, adopts 2000Lux intensity of illumination to irradiate conical flask.
Because the germination process of pollen needs various different material, in order to obtain the actual concentrations of each material, in culture medium, the concentration of each component can be determined by unitary variant.Such as, lilium pollen is carried out cultured in vitro by the culture medium be made up of sucrose and distilled water, passes through, change the concentration of sucrose, detect the germination rate of lilium pollen, thus obtain the best sucrose concentration of lilium pollen.Afterwards, configure the culture medium be made up of sucrose, water and boric acid, and in this culture medium, the concentration of sucrose being above-mentioned best sucrose concentration, by changing the concentration of boric acid, obtaining best boric acid concentration.
Active pollen nutrient solution can be obtained by said process, preferably, can also carry out obtained active pollen nutrient solution concentrated to remove moisture, with obtained concentrate.In the present embodiment, the concentrated rear volume of active pollen concentrate is 50% of the volume before concentrating.Further, obtained concentrate carries out deepfreeze, and refrigerated storage temperature is preferably 0 DEG C, is more preferably 1.5 DEG C, prevents the loss of nutritional labeling or is subject to extraneous pollution.
Embodiment two
Present embodiments provide a kind of preparation method of active pollen nutrient solution, comprise the following steps:
First, edible Hawthorn Pollen is gathered.
Secondly, configuration pin is to the edible culture medium of Hawthorn Pollen, and in the present embodiment, culture medium is composed as follows, counts by weight proportion:
Sucrose 15%
Boric acid 0.1%
Surplus is water.
Again, by utilizing culture medium to carry out germination in vitro to pollen, to prepare germination fluid.
Germination in vitro adopts constant-temperature shaking culture method particularly, the Hawthorn Pollen gathered is transferred in the conical flask after cleaning, sterilization, again culture medium is put into conical flask, vibration conical flask makes both fully mixing, is positioned over by conical flask in constant temperature vibration incubator and carries out cultured in vitro.Temperature in constant temperature vibration incubator is 25 DEG C, and incubation time is 1.5 hours, intensity of illumination is, the vibration frequency of conical flask is 200r/min.The germination rate of Hawthorn Pollen can be added up by decoration method, particularly, after pollen germination maturation, more starch can be produced, thus can dye by using I-KI (IKI) reagent, utilize dropper to get the Hawthorn Pollen solution some after germination in vitro in slide, then examine under a microscope, the germination rate of statistics Hawthorn Pollen.Preferably, in cultured in vitro process, in constant temperature vibration incubator, light irradiation apparatus is set, adopts 2600Lux intensity of illumination to irradiate conical flask.
Finally, ultrasonic process is carried out to germination fluid, obtain active pollen nutrient solution.By sonic oscillation process 9 minutes, producing ripple frequency was 15KHz, makes the precipitations such as the nutriment in pollen tube, thus obtained active pollen nutrient solution.
Obtain active pollen nutrient solution by said process, then the active pollen nutrient solution of high-quality can be obtained through concentrated and deepfreeze.Preferably, the volume after concentrated is 40% of original volume, and refrigerated storage temperature is preferably 2 DEG C.
Embodiment three
Present embodiments provide a kind of preparation method of active pollen nutrient solution, comprise the following steps:
First, gather edible rape pollen, the active pollen nutrient solution preparation method that the present embodiment provides also is applicable to make the active pollen nutrient solution based on greater celandine pollen, Pollen In Pinus Thunbergii Parl., zasiokaurin.
Secondly, configuration pin to the edible culture medium of Hawthorn Pollen, in the present embodiment, culture medium composed as follows, wherein by weight:
The physiologically active class material of trace can be added in culture medium, as VB1 and VB6 etc. with imitate the sprouting that promotes pollen or various mineral matter of can also readjusting prices in the medium first, as calcium nitrate.
Again, by utilizing culture medium to carry out germination in vitro to pollen, to obtain germination fluid.
Germination in vitro adopts constant-temperature shaking culture method, the Hawthorn Pollen gathered is transferred in the conical flask after cleaning, sterilization, again culture medium is put into conical flask, vibration conical flask makes both fully mixing, is positioned over by conical flask in constant temperature vibration incubator and carries out cultured in vitro.Temperature in constant temperature vibration incubator is 27 DEG C, and incubation time is 3 hours, intensity of illumination is, the vibration frequency of conical flask is 130r/min.The germination rate of Hawthorn Pollen can be added up by decoration method, particularly, after pollen germination maturation, more starch can be produced, thus can dye by using I-KI (IKI) reagent, utilize dropper to get the Hawthorn Pollen solution some after germination in vitro in slide, then examine under a microscope, the germination rate of statistics Hawthorn Pollen.Preferably, in cultured in vitro process, in constant temperature vibration incubator, light irradiation apparatus is set, adopts 3000Lux intensity of illumination to irradiate conical flask.
Finally, ultrasonic process is carried out to germination fluid, obtain active pollen nutrient solution.The time of ultrasonic process is 8 minutes, and ultrasonic frequency is 5KHz.Particularly, germination fluid is stored in container, such as beaker, conical flask etc., then puts it in ultrasonic device, by ultrasonic wave, germination fluid is carried out to the vibration of high frequency, separate out to make the active material in its pollen tube.Obtain active pollen nutrient solution by said process, then the active pollen nutrient solution of high-quality can be obtained through concentrated and deepfreeze.Preferably, the volume after concentrated is 60% of original volume, and refrigerated storage temperature is preferably 4 DEG C.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. a preparation method for active pollen nutrient solution, is characterized in that, comprises the following steps:
A gathers edible pollen;
B makes edible culture medium according to the kind of the pollen gathering gained, and described culture medium comprises the sucrose that part by weight is 5% ~ 35%;
C utilizes described culture medium to carry out germination in vitro to pollen, to obtain germination fluid;
D carries out ultrasonic process to germination fluid, to obtain active pollen nutrient solution.
2. the preparation method of active pollen nutrient solution according to claim 1, is characterized in that, the comprising of germination in vitro condition in step C: temperature 25 ~ 28 DEG C, time are 1 ~ 3 hour.
3. the preparation method of active pollen nutrient solution according to claim 1 and 2, is characterized in that, the germination in vitro condition in step C comprises: the intensity of illumination of 2000 ~ 3000Lux.
4. the preparation method of active pollen nutrient solution according to claim 1, is characterized in that, the pollen gathered in steps A is lilium pollen.
5. the preparation method of active pollen nutrient solution according to claim 4, is characterized in that, the culture medium in step B is composed as follows, wherein in part by weight:
Sucrose 12%
Boric acid 0.1%
Tryptophan 0.2%
Surplus is water.
6. the preparation method of active pollen nutrient solution according to claim 1, is characterized in that, the pollen gathered in steps A is Hawthorn Pollen.
7. the preparation method of active pollen nutrient solution according to claim 6, is characterized in that, the culture medium in step B is composed as follows, wherein in part by weight:
Sucrose 15%
Boric acid 0.1%
Surplus is water.
8. the preparation method of active pollen nutrient solution according to claim 1, is characterized in that, the pollen gathered in steps A is one or more in rape pollen, greater celandine pollen, Pollen In Pinus Thunbergii Parl., zasiokaurin.
9. the preparation method of active pollen nutrient solution according to claim 8, is characterized in that, the culture medium in step B is composed as follows, wherein in part by weight:
Sucrose 12% ~ 20%
Boric acid 0.1%
Compound amino acid 1%
Surplus is water.
10. the preparation method of active pollen nutrient solution according to claim 1, is characterized in that, further comprising the steps of after step D:
Obtained active pollen nutrient solution carries out concentrated to remove moisture by E, and concentrated volume ratio is 40% ~ 60%, with obtained concentrate;
Concentrate is carried out deepfreeze by F, and temperature is 0 ~ 4 DEG C.
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