CN102319267A - Method for efficiently preparing bee pollen aqueous extract - Google Patents
Method for efficiently preparing bee pollen aqueous extract Download PDFInfo
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- CN102319267A CN102319267A CN201110216647A CN201110216647A CN102319267A CN 102319267 A CN102319267 A CN 102319267A CN 201110216647 A CN201110216647 A CN 201110216647A CN 201110216647 A CN201110216647 A CN 201110216647A CN 102319267 A CN102319267 A CN 102319267A
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- aqueous extract
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Abstract
The invention discloses a method for efficiently preparing a bee pollen aqueous extract and relates to the technical field of processes for extracting effective materials from bee pollens. In the method, a coarse bee pollen aqueous extract is dissolved after being subjected to low-temperature freezing, because the specific gravity of the bee pollen aqueous extract is greater than that of a water molecule, after a process of standing and layering is carried out, the obtained lower-layer substance is a pure bee pollen aqueous extract in which large numbers of water molecules are removed, and the obtained upper-layer substance is water (containing less bee-pollen aqueous-extracts) which can be used for bee pollen extraction next time. By using the method disclosed by the invention, the concentration of effective active substances in the bee-pollen aqueous-extract is increased by 1-2 times, the extraction cost is reduced by more than 50%, and the biological activities are not affected. The pure bee pollen aqueous extract prepared by using the method disclosed by the invention can be directly prepared into various products with other excipients without being subjected to spray drying.
Description
Technical field
The present invention relates to from bee pollen, extract the Technology field of active substance.
Background technology
Prostate is an attached body of gland maximum in the male genetic urinary system, is positioned at the bladder below, and just as Semen Castaneae, its excretory prostatic fluid is the important component of seminal fluid.Prostatosis is called " cancer that is not cancer " by medical circle.
Fatty liver generally is because the decomposition and the anabolism disequilibrium of fat in the liver or transport the generation obstacle, fat will be in hepatocyte excess accumulation, when fat content increases to more than 30%, then be called fatty liver.The developed area sickness rate is higher, and morbidity has the trend of younger ones in an age groupization, has become the common hidden danger of a serious harm human health.Therefore, the control fatty liver has caused the great attention of medical circle.
Sexual precosity is meant that the child obviously shifts to an earlier date at the sexually matured age, and sexual precosity often follows body development to quicken, as health increase, muscle development is accelerated, epiphysis is closed in advance, and growth stops earlier.
The women that premature ovarian failure (POF) is meant normal development caused ovarian function failure with preceding because of certain reason at 40 years old.Except that receiving congenital heredity factor regulation and control, keeping of mature women's ovarian function also receives posteriori influence of various factors, and the true cause of its morbidity fully aware of not still up to now is so become the focus and the difficult point of domestic and international reproductive development circle to the research of this disease.
The inventor is contained the multiple water-soluble substances that can treat diseases such as prostate hyperplasia, fatty liver, sexual precosity and premature ovarian failure through systematic study discovery for many years in bee pollen, and realizes that the high efficiency extraction difficulty is very big.The elder generation that in the past proposed with bee pollen after the jet mill broken wall treatment; In normal-temperature water soak, ultrasonic Treatment, centrifuging and taking gets the bee pollen aqueous solution in High speed refrigerated centrifuge then, again with the bee pollen aqueous solution under 1～5 ℃ ambient temperature; Cross the 5000D molecular sieve; Can only obtain molecular weight is the thick bee pollen water extract below 5000, wherein contains large quantity of moisture, also needs spray-dried could the acquisition can be added on the material in the goods.
Summary of the invention
The invention reside in and invent the method for preparing that a kind of efficient production is used to treat disease bee pollen water extracts such as prostate hyperplasia, fatty liver, sexual precosity and premature ovarian failure.
The present invention includes following steps:
1) with bee pollen through the jet mill broken wall treatment;
2) under the condition of normal pressure, after the normal-temperature water immersion, ultrasonic Treatment;
3) be in 8000～16000 High speed refrigerated centrifuges that change centrifugal 10～15 minutes at rotating speed, obtain a bee pollen aqueous solution and a solid matter respectively;
4) with the bee pollen aqueous solution, under 1～5 ℃ ambient temperature, cross the 5000D molecular sieve, obtaining molecular weight is the thick bee pollen water extract below 5000;
5) said thick bee pollen water extract after freezing 24～48 hours, is dissolved standing demix at-20～-30 degree under 1～5 ℃ ambient temperature, take off a layer thing, promptly pure bee pollen water extract.
The present invention with thick bee pollen water extract freezing after again the dissolving; Because the proportion of bee pollen water extract is greater than the proportion of hydrone; Lower floor's thing of standing demix is the pure bee pollen water extract of removing large quantity of moisture; And upper layer substance is and contains the lower water of bee pollen water extract, also can be used as the usefulness of extracting bee pollen next time.Adopt the inventive method, can make that effective active matter concentration improves 1-2 doubly in the bee pollen water extract, reduce extraction cost more than 50%, and can not influence its BA.The pure bee pollen water extract that obtains through the present invention can be spray-dried again and be directly processed various products with other excipient.
The present invention also can make full use of the solid matter of first separation; That is: under the normal pressure, after a said solid matter and the volume ratio mixed soaking of normal-temperature water, through ultrasonic Treatment 10～20 minutes with 1 ︰ 1～4; Obtain secondary bee pollen aqueous solution with centrifugal separation again; Secondary bee pollen aqueous solution under 1～5 ℃ ambient temperature, is crossed the 5000D molecular sieve, and obtaining molecular weight is the thick bee pollen water extract below 5000.Solid matter is utilized again; Effective water soluble ingredient in the solid impurity that remains under for the first time centrifugal is further kept; When extracting bee pollen, use next time; Can effectively improve concentration of active substance in the bee pollen water extract, can improve and make in the bee pollen water extract effective active matter concentration improve 2～4 times.
In the step 1) according to the invention, the classification electric machine frequency of stream pulverizer is 55～60Hz, bleed pressure 0.7～1.0Mpa, and the processing time is 0.5～2 hour.Purpose is that the bee pollen conidial cell wall is destroyed, and is convenient to the extraction of active ingredient in the bee pollen and keeps active.
The specific embodiment
One, preparation bee pollen water extract:
1, go-on-go bee pollen (can be tea bee pollen or Brassica campestris L pollen or loose bee pollen or lotus bee pollen or pollen of Semen Fagopyri Esculenti or seed watermelon bee pollen): remove bee pollen impurity at normal temperatures.
2, place stream to carry out low-temperature airflow head-on collision broken wall treatment in the pulverizer 0.5～2 hour bee pollen, the classification electric machine frequency of control stream pulverizer is 55～60Hz, bleed pressure 0.7～1.0Mpa.
3, under the condition of normal pressure, soak 20～30 minutes with normal-temperature water after, ultrasonic Treatment 20～40 minutes.
4, be in 8000～16000 High speed refrigerated centrifuges that change centrifugal 10～15 minutes with the mixture after the ultrasonic Treatment at rotating speed, obtain bee pollen water solution A and solid matter B respectively.
5, under the normal pressure, after solid matter B and the volume ratio mixed soaking of normal-temperature water,, be in 8000～16000 High speed refrigerated centrifuges that change centrifugal 10～15 minutes at rotating speed again, obtain bee pollen aqueous solution B respectively through ultrasonic Treatment 10～20 minutes with 1 ︰ 1～4.
6, merge bee pollen water solution A and bee pollen aqueous solution B; After under-20～-30 ℃ of conditions freezing 24～48 hours, under 1～5 ℃ ambient temperature, dissolve standing demix, take off a layer thing; Be pure bee pollen water extract, supernatant is as the usefulness of extracting bee pollen next time.
Two, preparation oral formulations and ejection preparation:
1, preparation oral formulations A:
Pure bee pollen water extract and Mel or other food additive are mixed well, and after ultrafiltration and pasteurization sterilization, fill is subsequent use.
2, preparation ejection preparation B:
Pure bee pollen water extract pasteurization sterilization back is mixed with the volume ratio of 1 ︰ 1～2 with injecting normal saline, and the evacuation sealing is for use after the uniform dissolution.
Claims (3)
1. the high efficiency preparation method of bee pollen water extract may further comprise the steps:
1) with bee pollen through the jet mill broken wall treatment;
2) under the condition of normal pressure, after the normal-temperature water immersion, ultrasonic Treatment;
3) be in 8000～16000 High speed refrigerated centrifuges that change centrifugal 10～15 minutes at rotating speed, obtain a bee pollen aqueous solution and a solid matter respectively;
4) with the bee pollen aqueous solution, under 1～5 ℃ ambient temperature, cross the 5000D molecular sieve, obtaining molecular weight is the thick bee pollen water extract below 5000;
It is characterized in that: said thick bee pollen water extract after freezing 24～48 hours, is dissolved standing demix at-20～-30 degree under 1～5 ℃ ambient temperature, take off a layer thing, promptly pure bee pollen water extract.
2. according to the high efficiency preparation method of the said bee pollen water extract of claim 1; It is characterized in that: under the normal pressure, after a said solid matter and the volume ratio mixed soaking of normal-temperature water, through ultrasonic Treatment 10～20 minutes with 1 ︰ 1～4; Obtain secondary bee pollen aqueous solution with centrifugal separation again; Secondary bee pollen aqueous solution under 1～5 ℃ ambient temperature, is crossed the 5000D molecular sieve, and obtaining molecular weight is the thick bee pollen water extract below 5000.
3. according to the high efficiency preparation method of the said bee pollen water extract of claim 1, it is characterized in that: the classification electric machine frequency of stream pulverizer is 55～60Hz in the said step 1), bleed pressure 0.7～1.0Mpa, and the processing time is 0.5～2 hour.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011102166474A CN102319267B (en) | 2011-08-01 | 2011-08-01 | Method for efficiently preparing bee pollen aqueous extract |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2011102166474A CN102319267B (en) | 2011-08-01 | 2011-08-01 | Method for efficiently preparing bee pollen aqueous extract |
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| Publication Number | Publication Date |
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| CN102319267A true CN102319267A (en) | 2012-01-18 |
| CN102319267B CN102319267B (en) | 2013-04-24 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940653A (en) * | 2012-11-30 | 2013-02-27 | 扬州大学 | Bee pollen water extract buccal tablet for preventing and treating alcoholic fatty liver |
| CN103039752A (en) * | 2013-01-11 | 2013-04-17 | 江西旺大动物科技有限公司 | Immunization enhanced type piglet early-stage fodder |
| CN105053769A (en) * | 2015-07-10 | 2015-11-18 | 张彦文 | Preparation method of active pollen honey |
| CN105053768A (en) * | 2015-07-10 | 2015-11-18 | 张彦文 | Preparation method of active pollen nutrient solution |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119939A (en) * | 2010-12-28 | 2011-07-13 | 周斌 | Angiosperm bee pollen water extract for treating fatty liver and preparation method and application thereof |
-
2011
- 2011-08-01 CN CN2011102166474A patent/CN102319267B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119939A (en) * | 2010-12-28 | 2011-07-13 | 周斌 | Angiosperm bee pollen water extract for treating fatty liver and preparation method and application thereof |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940653A (en) * | 2012-11-30 | 2013-02-27 | 扬州大学 | Bee pollen water extract buccal tablet for preventing and treating alcoholic fatty liver |
| CN103039752A (en) * | 2013-01-11 | 2013-04-17 | 江西旺大动物科技有限公司 | Immunization enhanced type piglet early-stage fodder |
| CN105053769A (en) * | 2015-07-10 | 2015-11-18 | 张彦文 | Preparation method of active pollen honey |
| CN105053768A (en) * | 2015-07-10 | 2015-11-18 | 张彦文 | Preparation method of active pollen nutrient solution |
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| Publication number | Publication date |
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| CN102319267B (en) | 2013-04-24 |
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Address after: 225802 Jiangsu city of Yangzhou province Baoying County Jing Town Industrial Zone No. 14 crossing Patentee after: Zhou Bin Patentee after: Ye Manhong Address before: 225001 Jiangsu City, Yangzhou Old City Lane seven 18-3 Patentee before: Zhou Bin Patentee before: Ye Manhong |
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Address after: No. 8, Dashan Industrial Park, Gao Hong Kong, Taizhou, Jiangsu Co-patentee after: Ye Manhong Patentee after: Zhou Bin Address before: 225802 Jiangsu city of Yangzhou province Baoying County Jing Town Industrial Zone No. 14 crossing Co-patentee before: Ye Manhong Patentee before: Zhou Bin |
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