CN107647414A - A kind of instant bird's nest collagen Gly-His-Lys - Google Patents
A kind of instant bird's nest collagen Gly-His-Lys Download PDFInfo
- Publication number
- CN107647414A CN107647414A CN201710980792.7A CN201710980792A CN107647414A CN 107647414 A CN107647414 A CN 107647414A CN 201710980792 A CN201710980792 A CN 201710980792A CN 107647414 A CN107647414 A CN 107647414A
- Authority
- CN
- China
- Prior art keywords
- bird
- nest
- fine
- powder
- lys
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 244000000626 Daucus carota Species 0.000 title claims abstract description 165
- 235000005770 birds nest Nutrition 0.000 title claims abstract description 165
- 235000005765 wild carrot Nutrition 0.000 title claims abstract description 165
- 102000008186 Collagen Human genes 0.000 title claims abstract description 35
- 108010035532 Collagen Proteins 0.000 title claims abstract description 35
- 229920001436 collagen Polymers 0.000 title claims abstract description 35
- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 title claims abstract description 31
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 title claims abstract description 31
- 239000000843 powder Substances 0.000 claims abstract description 49
- 238000002156 mixing Methods 0.000 claims abstract description 39
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 239000002002 slurry Substances 0.000 claims abstract description 26
- 229920001184 polypeptide Polymers 0.000 claims abstract description 22
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 22
- 238000001035 drying Methods 0.000 claims abstract description 11
- 238000002360 preparation method Methods 0.000 claims abstract description 7
- 229920001353 Dextrin Polymers 0.000 claims abstract description 6
- 239000004375 Dextrin Substances 0.000 claims abstract description 6
- 238000004440 column chromatography Methods 0.000 claims abstract description 6
- 230000009514 concussion Effects 0.000 claims abstract description 6
- 235000019425 dextrin Nutrition 0.000 claims abstract description 6
- 239000007788 liquid Substances 0.000 claims description 25
- 239000008367 deionised water Substances 0.000 claims description 15
- 229910021641 deionized water Inorganic materials 0.000 claims description 15
- 238000001914 filtration Methods 0.000 claims description 15
- 239000002245 particle Substances 0.000 claims description 15
- 238000000034 method Methods 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 6
- 229920005654 Sephadex Polymers 0.000 claims description 5
- 239000012507 Sephadex™ Substances 0.000 claims description 5
- 230000002155 anti-virotic effect Effects 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 5
- 238000000227 grinding Methods 0.000 claims description 5
- 230000035939 shock Effects 0.000 claims description 5
- 238000000605 extraction Methods 0.000 abstract description 6
- 238000009835 boiling Methods 0.000 abstract description 3
- 238000000053 physical method Methods 0.000 abstract description 2
- 238000005538 encapsulation Methods 0.000 abstract 1
- 238000010298 pulverizing process Methods 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 238000000108 ultra-filtration Methods 0.000 abstract 1
- 238000001514 detection method Methods 0.000 description 4
- 241000209094 Oryza Species 0.000 description 3
- 235000007164 Oryza sativa Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 235000009566 rice Nutrition 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 230000035611 feeding Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- DWNBOPVKNPVNQG-LURJTMIESA-N (2s)-4-hydroxy-2-(propylamino)butanoic acid Chemical compound CCCN[C@H](C(O)=O)CCO DWNBOPVKNPVNQG-LURJTMIESA-N 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- PMMYEEVYMWASQN-DMTCNVIQSA-N Hydroxyproline Chemical compound O[C@H]1CN[C@H](C(O)=O)C1 PMMYEEVYMWASQN-DMTCNVIQSA-N 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000003796 beauty Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- PMMYEEVYMWASQN-UHFFFAOYSA-N dl-hydroxyproline Natural products OC1C[NH2+]C(C([O-])=O)C1 PMMYEEVYMWASQN-UHFFFAOYSA-N 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 229960002591 hydroxyproline Drugs 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000000813 small intestine Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- FGMPLJWBKKVCDB-UHFFFAOYSA-N trans-L-hydroxy-proline Natural products ON1CCCC1C(O)=O FGMPLJWBKKVCDB-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/14—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Nutrition Science (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Biochemistry (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of instant bird's nest collagen Gly-His-Lys, including following preparation process：Bird's nest is cleaned, air-dried, is ground, depth crushing, mixing slurry processed, wet pulverizing emulsify, dry, grind, slurrying, ultrasonic water bath concussion, centrifugal ultrafiltration, column chromatography collection, freeze-stable, drying, the mixing of addition dextrin, sterilization, encapsulation, present invention employs physical method to extract bird's nest collagen polypeptide, it ensure that the natural sex of extraction finished product, bird's nest collagen polypeptide powder is added to the water rapid dissolving, it is rapidly absorbed, the complicated stage of boiling is needed when solving edible bird's nest simultaneously, can preferably control edible component.
Description
Technical field
The present invention relates to the invention belongs to polypeptide field, more particularly to a kind of instant bird's nest collagen Gly-His-Lys.
Background technology
Collagen peptide is made up of two or two with upper amino acid, and up to 100%, collagen is rich in absorption rate
Glycine that human body needs, proline, propylhomoserin, the collagen such as hydroxyproline are most important composition portions in extracellular matrix
Point, collagen peptide is beauty and skin care field first choice articles for use, is to maintain skin and histoorgan form, the main component of structure,
And repair the important source material material of each injury tissue.
Bird's nest contains the polypeptide moiety and EGF beneficial to human body, can promote the metabolism of fat, directly stimulates
Cell division, regeneration, tissue reconstruction, nourishing, recovery to human body play very big effect.
Most people also rests on the method entirely decocted for bird's nest is edible at present, is passing through common boiling side in bird's nest
It is not high to the polypeptide absorption efficiency of inside not by broken wall treatment when method is eaten, eating effect is influenceed, causes to waste.
Bird's nest collagen peptide is the collagen peptide being extract from pure natural bird's nest, this collagen peptide
Bird's nest powder can be called.Pure natural bird's nest collagen peptide product is very few in the market, and part producing technology is coarse,
Composition Processing for removing is not done after with the addition of other chemical compositions during bird's nest extraction collagen peptide, destroys bird's nest collagen egg
The pure natural property of white Gly-His-Lys, and the bird's nest collagen Gly-His-Lys solubility extracted is slow, solubility is not high.
Bird's nest is deeply processed, using the method for physics, any other auxiliary substance is not added and carries out extraction, allows swallow
Out a kind of solubility of rear separation and Extraction is fast for protein release in nest, and the technology of the high collagen Gly-His-Lys of solubility there are no
Relevant report.
The content of the invention
In order to solve the above problems, the present invention, which adopts, provides a kind of instant bird's nest collagen Gly-His-Lys, and provides its preparation
Step.
A kind of instant bird's nest collagen Gly-His-Lys, including following preparation process：
（1）After bird's nest is cleaned up with deionized water, it is placed in dustless space and air-dries, air-dried temperature is maintained at 25~32 DEG C, obtains
Dry bird's nest；
（2）Above-mentioned dry bird's nest is placed in grinder, ground 10~20 minutes, is processed into the thick bird's nest powder of 30~200 mesh, by described in
Thick bird's nest powder puts ultrafine crusher into, is crushed to particle diameter at 3~15 μm using comminution by gas stream, obtains thin bird's nest
Powder；
（3）By the thin bird's nest powder:Deionized water in mass ratio 1:3~1:5 are positioned over 20~30 points of stirring in de-airing mixer
Clock, mixing speed are 90~100r/min, mixed pulp, obtain bird's nest mixing slurry；
（4）The bird's nest mixing slurry is placed in nanometer wet process disintegrator and crushes, emulsify 30~60 minutes, the bird's nest mixing slurry
By comprehensive functions such as multiple shear surface intensive shear, shock, grinding and holes in nanometer wet process disintegrator, particle is crushed to
Diameter obtains fine bird's nest mixing slurry between 20~150nm；
（5）The fine bird's nest mixing slurry is placed in drying machine and is dried, maintains the temperature between 30~40 DEG C, obtains pie
Bird's nest aggregate；
（5）The bird's nest aggregate is positioned over after being ground 1~2 hour in grinder and moves to ultrafine crusher depth crushing 1.5
Ultra-fine bird's nest fine powder of the particle diameter range in 20~200nm is obtained after hour, diameter range is 40 in the ultra-fine bird's nest fine powder
~100nm composition accounts for 70%~80%；
（6）By the ultra-fine bird's nest powder:Deionized water in mass ratio 1:5~1:10 carry out mixing to obtain ultra-fine bird's nest powder mixed liquor,
The ultra-fine bird's nest powder mixed liquor is positioned over progress ultrasonic water bath concussion, water bath time 2~4 hours, temperature model in water-bath tank
Enclose and be maintained at 60~80 DEG C；
（7）With band the upper ultra-fine bird's nest powder mixed liquor can be intercepted into the milipore filter of the dalton of molecular weight 8000, and to carry out centrifugation super
Filter, bird's nest refined filtration liquid is obtained after centrifugal filtration；
（8）The bird's nest refined filtration liquid is subjected to sephadex column chromatography, absworption peak component at 300nm is collected, obtains bird's nest polypeptide
Liquid；
（9）The bird's nest polypeptide liquid is placed in icebox and is refrigerated to -20 DEG C~-22 DEG C, and is kept for 2~4 hours；
（10）The bird's nest polypeptide liquid is placed in vacuum drier, control temperature 60 C carries out freeze-day with constant temperature, is obtained after drying
Bird's nest Gly-His-Lys；
（11）The bird's nest Gly-His-Lys and dextrin are pressed 1:4 mixing, ultraviolet antivirus post package obtain bird's nest collagen egg of the present invention
White Gly-His-Lys.
Instant bird's nest collagen Gly-His-Lys of the present invention, state and moieties content detection result are as follows：
Present invention employs physical method to extract bird's nest collagen polypeptide, it is not necessary to other chemical composition assisted extractions are added,
The natural sex of extraction finished product is ensure that, finished product molecular weight is small, is added to the water rapid dissolving, is transparent shape after dissolving, and bird's nest is thin
Born of the same parents' protein have passed through broken wall, be not required to digest, and directly absorb, and peptide is directly entered small intestine by oral cavity, stomach, and is rapidly absorbed,
Needed when being absorbed by the body rate up to 100% into blood circulation of human body system, organ and cell tissue, while solving edible bird's nest
The complicated stage of boiling is wanted, can preferably control edible component.
Bird's nest collagen polypeptide of the present invention, by Kunming kind sodoku drug test, mouse weighs 20~25g, female
Each 5, by mouse appetite at utmost feeding, 2 times a day, after two weeks continuous feedings, Continuous Observation 1 month is without exception.
The embodiment of the present invention is described further below, herein it should be noted that for these implementations
The explanation of mode is used to help understand the present invention, but does not form limitation of the invention, in addition, invention described below
As long as involved technical characteristic does not form conflict can and is combined with each other each other in each Example embodiments.
Embodiment 1：
A kind of instant bird's nest collagen Gly-His-Lys, including following preparation process：
（1）After bird's nest is cleaned up with deionized water, it is placed in dustless space and air-dries, air-dried temperature is maintained at 25 DEG C, obtains dry swallow
Nest；
（2）Above-mentioned dry bird's nest is placed in grinder, grinds 20 minutes, the thick bird's nest powder of 30~200 mesh is processed into, by the thick swallow
Nest powder puts ultrafine crusher into, is crushed to particle diameter at 3~15 μm using comminution by gas stream, obtains thin bird's nest powder；
（3）By the thin bird's nest powder:Deionized water in mass ratio 1:3 are positioned in de-airing mixer and stir 30 minutes, stirring speed
Spend for 100r/min, mixed pulp, obtain bird's nest mixing slurry；
（4）The bird's nest mixing slurry is placed in nanometer wet process disintegrator and crushes, emulsify 30 minutes, the bird's nest mixing slurry is being received
By comprehensive functions such as multiple shear surface intensive shear, shock, grinding and holes in rice wet process disintegrator, particle diameter is crushed to
Between 20~150nm, fine bird's nest mixing slurry is obtained；
（5）The fine bird's nest mixing slurry is placed in drying machine and is dried, maintains the temperature between 30~40 DEG C, obtains pie
Bird's nest aggregate；
（5）The bird's nest aggregate is positioned over after being ground 2 hours in grinder and moves to ultrafine crusher depth crushing 1.5 hours
Ultra-fine bird's nest fine powder of the particle diameter range in 20~200nm is obtained afterwards；
（6）By the ultra-fine bird's nest powder:Deionized water in mass ratio 1:10 carry out mixing to obtain ultra-fine bird's nest powder mixed liquor, by described in
Ultra-fine bird's nest powder mixed liquor is positioned over progress ultrasonic water bath concussion in water-bath tank, and water bath time 4 hours, temperature range is maintained at 60
℃；
（7）With band the upper ultra-fine bird's nest powder mixed liquor can be intercepted into the milipore filter of the dalton of molecular weight 8000, and to carry out centrifugation super
Filter, bird's nest refined filtration liquid is obtained after centrifugal filtration；
（8）The bird's nest refined filtration liquid is subjected to sephadex column chromatography, absworption peak component at 300nm is collected, obtains bird's nest polypeptide
Liquid；
（9）The bird's nest polypeptide liquid is placed in icebox and is refrigerated to -22 DEG C, and is kept for 2 hours；
（10）The bird's nest polypeptide liquid is placed in vacuum drier, control temperature 60 C carries out freeze-day with constant temperature, is obtained after drying
Bird's nest Gly-His-Lys；
（11）The bird's nest Gly-His-Lys and dextrin are pressed 1:4 mixing, ultraviolet antivirus post package obtain bird's nest collagen egg of the present invention
White Gly-His-Lys.
By bird's nest collagen peptide manufactured in the present embodiment, state and moieties content detection result are as follows：
Embodiment 2：
A kind of instant bird's nest collagen Gly-His-Lys, including following preparation process：
（1）After bird's nest is cleaned up with deionized water, it is placed in dustless space and air-dries, air-dried temperature is maintained at 25~32 DEG C, obtains
Dry bird's nest；
（2）Above-mentioned dry bird's nest is placed in grinder, grinds 10 minutes, the thick bird's nest powder of 30~200 mesh is processed into, by the thick swallow
Nest powder puts ultrafine crusher into, is crushed to particle diameter at 3~15 μm using comminution by gas stream, obtains thin bird's nest powder；
（3）By the thin bird's nest powder:Deionized water in mass ratio 1:5 are positioned in de-airing mixer and stir 25 minutes, stirring speed
Spend for 90r/min, mixed pulp, obtain bird's nest mixing slurry；
（4）The bird's nest mixing slurry is placed in nanometer wet process disintegrator and crushes, emulsify 50 minutes, the bird's nest mixing slurry is being received
By comprehensive functions such as multiple shear surface intensive shear, shock, grinding and holes in rice wet process disintegrator, particle diameter is crushed to
Between 20~150nm, fine bird's nest mixing slurry is obtained；
（5）The fine bird's nest mixing slurry is placed in drying machine and is dried, maintains the temperature between 30~40 DEG C, obtains pie
Bird's nest aggregate；
（5）The bird's nest aggregate is positioned over after being ground 1.2 hours in grinder move to ultrafine crusher depth crush it is 1.5 small
When after particle diameter range 20~200nm ultra-fine bird's nest fine powder；
（6）By the ultra-fine bird's nest powder:Deionized water in mass ratio 1:7 carry out mixing to obtain ultra-fine bird's nest powder mixed liquor, by described in
Ultra-fine bird's nest powder mixed liquor is positioned over progress ultrasonic water bath concussion in water-bath tank, and water bath time 3 hours, temperature range is maintained at 65
℃；
（7）With band the upper ultra-fine bird's nest powder mixed liquor can be intercepted into the milipore filter of the dalton of molecular weight 8000, and to carry out centrifugation super
Filter, bird's nest refined filtration liquid is obtained after centrifugal filtration；
（8）The bird's nest refined filtration liquid is subjected to sephadex column chromatography, absworption peak component at 300nm is collected, obtains bird's nest polypeptide
Liquid；
（9）The bird's nest polypeptide liquid is placed in icebox and is refrigerated to -20.5 DEG C, and is kept for 3 hours；
（10）The bird's nest polypeptide liquid is placed in vacuum drier, control temperature 60 C carries out freeze-day with constant temperature, is obtained after drying
Bird's nest Gly-His-Lys；
（11）The bird's nest Gly-His-Lys and dextrin are pressed 1:4 mixing, ultraviolet antivirus post package obtain bird's nest collagen egg of the present invention
White Gly-His-Lys.
Instant bird's nest collagen Gly-His-Lys of the present invention, state and moieties content detection result are as follows：
Embodiment 3：
A kind of instant bird's nest collagen Gly-His-Lys, including following preparation process：
（1）After bird's nest is cleaned up with deionized water, it is placed in dustless space and air-dries, air-dried temperature is maintained at 30 DEG C, obtains dry swallow
Nest；
（2）Above-mentioned dry bird's nest is placed in grinder, grinds 20 minutes, the thick bird's nest powder of 30~200 mesh is processed into, by the thick swallow
Nest powder puts ultrafine crusher into, is crushed to particle diameter at 3~15 μm using comminution by gas stream, obtains thin bird's nest powder；
（3）By the thin bird's nest powder:Deionized water in mass ratio 1:5 are positioned in de-airing mixer and stir 20 minutes, stirring speed
Spend for 100r/min, mixed pulp, obtain bird's nest mixing slurry；
（4）The bird's nest mixing slurry is placed in nanometer wet process disintegrator and crushes, emulsify 45 minutes, the bird's nest mixing slurry is being received
By comprehensive functions such as multiple shear surface intensive shear, shock, grinding and holes in rice wet process disintegrator, particle diameter is crushed to
Between 20~150nm, fine bird's nest mixing slurry is obtained；
（5）The fine bird's nest mixing slurry is placed in drying machine and is dried, maintains the temperature between 30~40 DEG C, obtains pie
Bird's nest aggregate；
（5）The bird's nest aggregate is positioned over after being ground 1.5 hours in grinder move to ultrafine crusher depth crush it is 1.5 small
When after particle diameter range 20~200nm ultra-fine bird's nest fine powder；
（6）By the ultra-fine bird's nest powder:Deionized water in mass ratio 1:5 carry out mixing to obtain ultra-fine bird's nest powder mixed liquor, by described in
Ultra-fine bird's nest powder mixed liquor is positioned over progress ultrasonic water bath concussion in water-bath tank, and water bath time 2 hours, temperature range is maintained at 75
℃；
（7）With band the upper ultra-fine bird's nest powder mixed liquor can be intercepted into the milipore filter of the dalton of molecular weight 8000, and to carry out centrifugation super
Filter, bird's nest refined filtration liquid is obtained after centrifugal filtration；
（8）The bird's nest refined filtration liquid is subjected to sephadex column chromatography, absworption peak component at 300nm is collected, obtains bird's nest polypeptide
Liquid；
（9）The bird's nest polypeptide liquid is placed in icebox and is refrigerated to -20 DEG C, and is kept for 4 hours；
（10）The bird's nest polypeptide liquid is placed in vacuum drier, control temperature 60 C carries out freeze-day with constant temperature, is obtained after drying
Bird's nest Gly-His-Lys；
（11）The bird's nest Gly-His-Lys and dextrin are pressed 1:4 mixing, ultraviolet antivirus post package obtain bird's nest collagen egg of the present invention
White Gly-His-Lys.
Instant bird's nest collagen Gly-His-Lys of the present invention, state and moieties content detection result are as follows：
Claims (1)
1. a kind of instant bird's nest collagen Gly-His-Lys, including following preparation process：
（1）After bird's nest is cleaned up with deionized water, it is placed in dustless space and air-dries, air-dried temperature is maintained at 25~32 DEG C, obtains
Dry bird's nest；
（2）Above-mentioned dry bird's nest is placed in grinder, ground 10~20 minutes, is processed into the thick bird's nest powder of 30~200 mesh, by described in
Thick bird's nest powder puts ultrafine crusher into, is crushed to particle diameter at 3~15 μm using comminution by gas stream, obtains thin bird's nest
Powder；
（3）By the thin bird's nest powder:Deionized water in mass ratio 1:3~1:5 are positioned over 20~30 points of stirring in de-airing mixer
Clock, mixing speed are 90~100r/min, mixed pulp, obtain bird's nest mixing slurry；
（4）The bird's nest mixing slurry is placed in nanometer wet process disintegrator and crushes, emulsify 30~60 minutes, the bird's nest mixing slurry
By comprehensive functions such as multiple shear surface intensive shear, shock, grinding and holes in nanometer wet process disintegrator, particle is crushed to
Diameter obtains fine bird's nest mixing slurry between 20~150nm；
（5）The fine bird's nest mixing slurry is placed in drying machine and is dried, maintains the temperature between 30~40 DEG C, obtains pie
Bird's nest aggregate；
（5）The bird's nest aggregate is positioned over after being ground 1~2 hour in grinder and moves to ultrafine crusher depth crushing 1.5
Ultra-fine bird's nest fine powder of the particle diameter range in 20~200nm is obtained after hour, diameter range is 40 in the ultra-fine bird's nest fine powder
~100nm composition accounts for 70%~80%；
（6）By the ultra-fine bird's nest powder:Deionized water in mass ratio 1:5~1:10 carry out mixing to obtain ultra-fine bird's nest powder mixed liquor,
The ultra-fine bird's nest powder mixed liquor is positioned over progress ultrasonic water bath concussion, water bath time 2~4 hours, temperature model in water-bath tank
Enclose and be maintained at 60~80 DEG C；
（7）With band the upper ultra-fine bird's nest powder mixed liquor can be intercepted into the milipore filter of the dalton of molecular weight 8000, and to carry out centrifugation super
Filter, bird's nest refined filtration liquid is obtained after centrifugal filtration；
（8）The bird's nest refined filtration liquid is subjected to sephadex column chromatography, absworption peak component at 300nm is collected, obtains bird's nest polypeptide
Liquid；
（9）The bird's nest polypeptide liquid is placed in icebox and is refrigerated to -20 DEG C~-22 DEG C, and is kept for 2~4 hours；
（10）The bird's nest polypeptide liquid is placed in vacuum drier, control temperature 60 C carries out freeze-day with constant temperature, is obtained after drying
Bird's nest Gly-His-Lys；
（11）The bird's nest Gly-His-Lys and dextrin are pressed 1:4 mixing, ultraviolet antivirus post package obtain bird's nest collagen egg of the present invention
White Gly-His-Lys.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710980792.7A CN107647414A (en) | 2017-10-20 | 2017-10-20 | A kind of instant bird's nest collagen Gly-His-Lys |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710980792.7A CN107647414A (en) | 2017-10-20 | 2017-10-20 | A kind of instant bird's nest collagen Gly-His-Lys |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107647414A true CN107647414A (en) | 2018-02-02 |
Family
ID=61119171
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710980792.7A Pending CN107647414A (en) | 2017-10-20 | 2017-10-20 | A kind of instant bird's nest collagen Gly-His-Lys |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107647414A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902940A (en) * | 2018-06-28 | 2018-11-30 | 燕生（福建）生物工程有限公司 | A kind of instant bird's nest collagen Gly-His-Lys and preparation method thereof |
| CN108968077A (en) * | 2018-07-13 | 2018-12-11 | 杭州相生相成科技有限公司 | A kind of elastin laminin Gly-His-Lys and preparation method thereof |
| CN109527586A (en) * | 2019-01-03 | 2019-03-29 | 拓普河北生物科技有限公司 | A kind of preparation method of compound micromolecular collagen peptide powder |
| CN110257459A (en) * | 2019-05-16 | 2019-09-20 | 广西慧宝源医药科技有限公司 | Bird's nest small-molecular peptides and its instant Gly-His-Lys |
| CN110484583A (en) * | 2019-07-10 | 2019-11-22 | 广西慧宝源医药科技有限公司 | Bird's nest active peptide and its antitumor application thereof |
| WO2022032803A1 (en) * | 2020-08-10 | 2022-02-17 | 广西自贸区燕握生物科技有限公司 | Extraction method for edible bird's nest, and extract and product thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895273A (en) * | 2006-06-20 | 2007-01-17 | 陈包荣 | Preparation of nano-bird's nest microparticle |
| CN103431384A (en) * | 2013-08-20 | 2013-12-11 | 湖南省皇媛世家生命科技有限公司 | Preparation method of nano bird's nest particles |
| CN104172153A (en) * | 2014-07-03 | 2014-12-03 | 大连海晏堂生物有限公司 | Instant cubilose product and preparation method thereof |
-
2017
- 2017-10-20 CN CN201710980792.7A patent/CN107647414A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1895273A (en) * | 2006-06-20 | 2007-01-17 | 陈包荣 | Preparation of nano-bird's nest microparticle |
| CN103431384A (en) * | 2013-08-20 | 2013-12-11 | 湖南省皇媛世家生命科技有限公司 | Preparation method of nano bird's nest particles |
| CN104172153A (en) * | 2014-07-03 | 2014-12-03 | 大连海晏堂生物有限公司 | Instant cubilose product and preparation method thereof |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108902940A (en) * | 2018-06-28 | 2018-11-30 | 燕生（福建）生物工程有限公司 | A kind of instant bird's nest collagen Gly-His-Lys and preparation method thereof |
| CN108968077A (en) * | 2018-07-13 | 2018-12-11 | 杭州相生相成科技有限公司 | A kind of elastin laminin Gly-His-Lys and preparation method thereof |
| CN109527586A (en) * | 2019-01-03 | 2019-03-29 | 拓普河北生物科技有限公司 | A kind of preparation method of compound micromolecular collagen peptide powder |
| CN110257459A (en) * | 2019-05-16 | 2019-09-20 | 广西慧宝源医药科技有限公司 | Bird's nest small-molecular peptides and its instant Gly-His-Lys |
| CN110257459B (en) * | 2019-05-16 | 2021-04-13 | 广西慧宝源医药科技有限公司 | Bird's nest small molecular peptide and instant peptide powder thereof |
| CN110484583A (en) * | 2019-07-10 | 2019-11-22 | 广西慧宝源医药科技有限公司 | Bird's nest active peptide and its antitumor application thereof |
| WO2022032803A1 (en) * | 2020-08-10 | 2022-02-17 | 广西自贸区燕握生物科技有限公司 | Extraction method for edible bird's nest, and extract and product thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107647414A (en) | A kind of instant bird's nest collagen Gly-His-Lys | |
| CN103735782A (en) | Sea cucumber intestine extract composite soft capsules and preparation method thereof | |
| CN101429254A (en) | Bletilla striata polysaccharide, preparation method and new uses thereof | |
| CN105831757A (en) | Preparation method of deer skin gelatin powder | |
| CN104152521A (en) | Paeonia suffruticosa pollen protein polypeptide and preparation method and application thereof | |
| CN112007057B (en) | Preparation method of wall-broken ganoderma lucidum spore powder capsule and wall-broken ganoderma lucidum spore powder capsule | |
| CN109331038A (en) | A kind of production technology of deer haemin and its preparation method of health care product | |
| CN105614848A (en) | Nutritious tartary buckwheat breakfast powder suitable for females and preparation method of breakfast powder | |
| CN106070863A (en) | A kind of Pseudobulbus Bletillae (Rhizoma Bletillae) scented tea and preparation method thereof | |
| CN107752005A (en) | A kind of pilose antler double enzymolysis extracting method | |
| CN103223104B (en) | A kind of Chinese medicine composition treating diabetes and preparation method thereof | |
| CN100389679C (en) | A deer bone granule | |
| CN107495388A (en) | A kind of preparation method of bird's nest polypeptide powder | |
| CN112655834A (en) | Piglet health-care feed additive composition and application thereof | |
| CN105341839A (en) | Preparation method of snakegourd fruit kernel refined powder capable of clearing heat | |
| CN104383262B (en) | A kind of capsule for strengthening immunologic function | |
| CN101554473A (en) | Method for preparing compound with hypoglycemic function containing cornu cervi pantotrichum insulin-like active factors | |
| JP2003088323A (en) | Method for extracting active ingredient from solid material with hard surface and edible composition containing the active ingredient | |
| CN112168932A (en) | Traditional Chinese medicine composition and extract for preventing new coronary pneumonia, and preparation method and application thereof | |
| CN101416985A (en) | Extraction method of arca extract freeze-drying powder | |
| CN109294984A (en) | A kind of Lentinan and preparation method thereof of internal efficient amplification NK cell | |
| CN109043028A (en) | The instant tea and preparation method thereof of U.S. rattan fruit shell | |
| RU2568586C1 (en) | Method of producing dry extract of bupleurum aureum | |
| CN107581512A (en) | A kind of preparation method of soluble Penis et testis cervi extract | |
| KR102332638B1 (en) | Method of manufacturing liquid type product comprising powder of whole platycodon |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20180202 |
|
| WD01 | Invention patent application deemed withdrawn after publication |