CN101416985A - Extraction method of arca extract freeze-drying powder - Google Patents
Extraction method of arca extract freeze-drying powder Download PDFInfo
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- CN101416985A CN101416985A CNA2008102391377A CN200810239137A CN101416985A CN 101416985 A CN101416985 A CN 101416985A CN A2008102391377 A CNA2008102391377 A CN A2008102391377A CN 200810239137 A CN200810239137 A CN 200810239137A CN 101416985 A CN101416985 A CN 101416985A
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Abstract
The invention relates to a method for extracting a clam extract freeze-dried powder. The method is characterized by comprising the following steps: cleaning: fresh marine organism clams are cleaned to remove sludge, shells are removed, soft bodies are taken out and cleaned until no bloodiness is left and then drained, thus obtaining a clean material; mashing: particle size is between 0.2cm and 0.4cm; extracting: the clean material mashed is mixed with water by a ratio of 1:1 to 1:2 in an extraction plant for extraction for 2 hours to 6 hours at temperature between 0 DEG C and 10 DEG C, separation, centrifugation and deslagging are conducted, thus obtaining a centrifugate; and freeze-drying: the clam extract freeze-dried powder is obtained through pre-freezing and first phase and second phase of sublimation. The extraction method is simple and effective, has effective components with the high content of bioactive proteins and is used for producing clam extract enteric-coated capsules to treat early, intermediate stage and terminal cancer patients such as lung cancer, liver cancer, renal carcinoma, carcinoma ventriculi, cervical carcinoma patients and the like.
Description
Technical field
The present invention relates to marine organisms and extract the field, particularly relate to the extracting method of the arca extract freeze-drying powder of marine organisms arca extract.
Background technology
The ocean be covered with earth surface long-pending 71%, the sea water cumulative volume takes up an area of 97% of ball Total Water, still unemployed ocean is bigger 5～10 times than land with the effective area of seashore, a large amount of marine organisms are living in the ocean, resource is extremely abundant, and marine organisms are survival and developments in the high salt specific environment in ocean, than Lu Yuan biology its unique features and pharmacologically active are arranged, be present contaminated in the world few living resources, be the new resources that obtain bioactive substance, the exploitation marine natural product more and more causes the great attention of countries in the world the world of medicine, develops research one after another, and it is the future of marine drug industrial development.
Marine organisms are various in style, different uniqueness, and wherein Carnis Arca inflata class biology is one of extremely abundant marine organisms, belong to Mollusca, according to the Chinese medicine voluminous dictionary put down in writing that its nature and flavor are sweet, warm, tool is enriched blood, warming middle-JIAO, the merit that is good for the stomach, cure mainly blood deficiency flaccidity syndrome and arthralgia syndrome, stomachache, dyspepsia, diseases such as dysentery pus and blood." medical center bun will " annotates " Carnis Arca inflata meat reinforcing the heart blood, dissipating blood stasis blood, the expectorant of broken knot." its meat contains crude protein 15.79%, crude fat 0.45%, glycogen 1.067%, per 100 grams contain vitamin A 400 ius, vitamin B
1200ug, vitamin B
2200ug, and several amino acids such as nicotinic acid, taurine have very high medical value, and going deep into this uniqueness of developmental research Carnis Arca inflata class, to enrich resource medicine significant.
Up to now, extract the active drug composition, do not see report abroad as yet from ocean Carnis Arca inflata apoplexy due to endogenous wind.Domestic existing document and patent report, Chinese patent CN1600792A disclose the polypeptide protein class antitumor effective ingredient in the Scapharca subcrenata, and extract separates intercepting molecular weight 3～150KDa composition through gel filtration chromatography again after ion-exchange chromatography, desalination.Chinese patent CN1857717A and Chinese Journal of Marine Drugs V0123, No5, P35-37 discloses a kind of Carnis Arca inflata class antineoplaston extract, and the antitumaous effect of injection, extracting method is through ammonium sulfate precipitation, the Superdex75 gel permeation chromatography separates and desalination prepares peptides, molecular weight 3000Dal; Its injection is used for various treatment for cancer, and carries out the research of mouse anti tumor effect.In the clinical practice, oral administration route is more convenient easily than drug administration by injection.
Summary of the invention
The object of the invention is to overcome above-mentioned prior art and has not enough and present marine pharmaceutical resource scarcity, be engaged in developmental research and the clinical experience that marine organisms are extracted for a long time through the inventor, develop a kind of biological activity height, be convenient to oral, safe without toxic side effect, the extracting method of eutherapeutic a kind of arca extract freeze-drying powder.
The extracting method of a kind of arca extract freeze-drying powder provided by the invention comprises the following steps:
1. clean
With fresh and alive marine organisms Carnis Arca inflata class, be preferably Scapharca subcrenata, clean dirt with drinking water, the reuse purification is washed repeatedly, shells and gets software (soft tissue or meat), and the reuse purified water is washed till no blood stains drain well and gets material only.
2. smash to pieces
Step clean material is 1. smashed to pieces, and smashing granularity (particle diameter) to pieces is 0.2～0.4cm.
3. extract
The step material of smashing to pieces is 2. mixed with purified water, smashing material and purified water weight ratio to pieces is 1:1 to 1:2, preferred 1:1.5 stirs extraction 1 time or 2 times, preferred 1 time in 0-10 ℃ in extraction element, each extraction time is 2-6 hour, preferred 4 hours, 50～100 rev/mins of mixing speeds, separation and Extraction liquid, carry out again centrifugal (as adopting GQ105 high speed tube centrifuge, 20000 rev/mins) remove slag centrifugal liquid.
4. lyophilizing
Step centrifugal liquid is 3. put into freeze drying box, and the pre-freeze temperature is-20 to-40 ℃, preferred-30～-35 ℃, cooling rate is 20-40 ℃/h, be preferably 30 ℃/h, be incubated 3-5 hour, be preferably 4 hours, the first phase sublimation temperature is 5-15 ℃, be preferably 10 ℃, programming rate is 5-15 ℃/h, is preferably 10 ℃/h, temperature retention time is 2-6 hour, is preferably 4 hours; 20-40 ℃ of second phase sublimation temperature is preferably 30 ℃, and programming rate is 10-20 ℃/h, is preferably 15 ℃/h, and temperature retention time is 4-8 hour, is preferably 6 hours, gets arca extract freeze-drying powder (abbreviation lyophilized powder).Arca extract freeze-drying powder is fallow loose block, and it is little salty to distinguish the flavor of, little raw meat,
Loss on drying: 1.0-9.0 weight %.
Residue on ignition: 1.0-12 weight %.
Limit test of microbe: up to specification (" 2005 editions appendix XIII checks of Chinese pharmacopoeia).
Glucose content: 20-50mg/g.
The test of nitrogen content: 55-95mg/g (the above Chinese Pharmacopoeia 2005 of pressing) version regulation.
In the extracting method according to arca extract freeze-drying powder provided by the invention, described Carnis Arca inflata class is Scapharca subcrenata, Tegillarca granosa, Scapharca broughtonii etc., is preferably Scapharca subcrenata.Arca extract freeze-drying powder is the extract of marine organisms Carnis Arca inflata class, its active ingredient is a protein substance, the biologically active protein substance carries out extraction separation for the ease of extracting active ingredient biological activity protein class material under low temperature 0-10 ℃ condition.Marine organisms Carnis Arca inflata class is got software and will be smashed to pieces earlier through cleaning to shell, smash granularity (particle diameter) to pieces and be 0.2-0.4cm, because if the excessive relatively stripping that is unfavorable for active ingredient of granularity, if the too small relatively extracting solution that influences of granularity separates and can make follow-up centrifugal link increase load simultaneously.Extraction time is unsuitable long or too short, extract 1 time or 2 times, each extraction time 2-6 hour, be preferably 4 hours, if extraction time is too short, active ingredient is extracted not exclusively, and overlong time can make to be extracted composition and longly cause further decomposition (because this product effective site is protein-based composition) in space open-assembly time.
The extracting method characteristics of arca extract freeze-drying powder provided by the invention:
Extraction process is simply effective, extracts effective biological activity protein class material height, and yield is 6% of clean material (the Scapharca subcrenata software is cleaned and drained material) weight.
Make arca extract enteric-coated capsules to early, middle and late phase cancer patient for example pulmonary carcinoma, hepatocarcinoma, gastric cancer renal carcinoma and cervical cancer etc. can obviously improve symptom, dwindle the tumor body, improve life quality, prolong life cycle.
The specific embodiment
The present invention further specifies the present invention with the following example, but protection scope of the present invention is not limited to the following example.
Embodiment 1
Get fresh and alive Scapharca subcrenata 400Kg, washing repeatedly to shell surface with drinking water does not have dirt, the reuse purified water is cleaned three times, shell and get soft tissue and clean to there not being blood stains with purified water, drain the water and get clean material and weigh, clean material is smashed to pieces through bruisher, and granularity is 0.3cm, adds purified water by weight (w/w) 1:1.5,0-10 ℃ with blender at a slow speed (60r/min) stir to extract 4 hours, centrifugal the removing slag of extraction element GQ105 high speed tube centrifuge (20000r/min) carried out lyophilizing, gets the lyophilized powder raw material, mix homogeneously, vacuum packaging, cold closet is placed, and the result is as follows:
Table 1
Embodiment 2-3
Embodiment 2-3 manufacturing step is same as embodiment 1, and it is listed in above-mentioned table 1 not to be both extraction process.
Claims (2)
1, a kind of extracting method of arca extract freeze-drying powder comprises the following steps:
1. clean
With fresh and alive marine organisms Carnis Arca inflata, clean dirt with drinking water, with the purification washing repeatedly shelling and get software in the back, is washed till no blood stains drain well with purified water and gets material only;
2. step clean material is 1. smashed to pieces, smashing granularity to pieces is 0.2～0.4cm;
3. extract
2. smash step to pieces material and mix with purified water, smash material to pieces and the purified water weight ratio is 1:1 to 1:2, stir in 0～10 ℃ in extraction element and extract 1 time or 2 times, be 2-6 hour at every turn, separation and Extraction liquid, carry out again centrifugal remove slag centrifugal liquid;
4. lyophilizing
Step centrifugal liquid is 3. put into freeze drying box, the pre-freeze temperature is-20 to-40 ℃, cooling rate is 20-40 ℃/h, is incubated 3-5 hour, and the first phase sublimation temperature is 5-15 ℃, programming rate is 5-15 ℃/h, temperature retention time is 2-6 hour, and the second stage of sublimation temperature 20-40 ℃, programming rate is 10-20 ℃/h, temperature retention time is 4-8 hour, gets arca extract freeze-drying powder.
2, according to the extracting method of the arca extract freeze-drying powder of claim 1, it is characterized in that described extraction time is 1 time, 4 hours extraction times; Describedly smash material to pieces and the purified water weight ratio is 1:1.5; Pre-freeze temperature-30～-35 ℃, 30 ℃ of cooling rates per hour are incubated 4 hours, 10 ℃ of first phase sublimation temperatures, 10 ℃/h of programming rate, temperature retention time 4 hours, 30 ℃ of the second stage of sublimation temperatures, 15 ℃/h of programming rate, temperature retention time 6 hours.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008102391377A CN101416985A (en) | 2008-12-10 | 2008-12-10 | Extraction method of arca extract freeze-drying powder |
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| CNA2008102391377A CN101416985A (en) | 2008-12-10 | 2008-12-10 | Extraction method of arca extract freeze-drying powder |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106243236A (en) * | 2016-07-28 | 2016-12-21 | 石雷 | A kind of preparation method of blood clam extract product |
| US11604026B2 (en) | 2019-03-14 | 2023-03-14 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
| US11634257B2 (en) | 2017-10-09 | 2023-04-25 | Terumo Bct Biotechnologies, Llc | Lyophilization container and method of using same |
-
2008
- 2008-12-10 CN CNA2008102391377A patent/CN101416985A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106243236A (en) * | 2016-07-28 | 2016-12-21 | 石雷 | A kind of preparation method of blood clam extract product |
| CN106243236B (en) * | 2016-07-28 | 2018-12-07 | 石雷 | A kind of preparation method of blood clam extract product |
| US11634257B2 (en) | 2017-10-09 | 2023-04-25 | Terumo Bct Biotechnologies, Llc | Lyophilization container and method of using same |
| US11604026B2 (en) | 2019-03-14 | 2023-03-14 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
| US11609043B2 (en) | 2019-03-14 | 2023-03-21 | Terumo Bct Biotechnologies, Llc | Lyophilization container fill fixture, system and method of use |
| US11609042B2 (en) | 2019-03-14 | 2023-03-21 | Terumo Bct Biotechnologies, Llc | Multi-part lyophilization container and method of use |
| US11740019B2 (en) | 2019-03-14 | 2023-08-29 | Terumo Bct Biotechnologies, Llc | Lyophilization loading tray assembly and system |
| US11747082B2 (en) | 2019-03-14 | 2023-09-05 | Terumo Bct Biotechnologies, Llc | Multi-part lyophilization container and method of use |
| US11815311B2 (en) | 2019-03-14 | 2023-11-14 | Terumo Bct Biotechnologies, Llc | Lyophilization container fill fixture, system and method of use |
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Application publication date: 20090429 |