CN102319267B - Method for efficiently preparing bee pollen aqueous extract - Google Patents
Method for efficiently preparing bee pollen aqueous extract Download PDFInfo
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- CN102319267B CN102319267B CN2011102166474A CN201110216647A CN102319267B CN 102319267 B CN102319267 B CN 102319267B CN 2011102166474 A CN2011102166474 A CN 2011102166474A CN 201110216647 A CN201110216647 A CN 201110216647A CN 102319267 B CN102319267 B CN 102319267B
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Abstract
The invention discloses a method for efficiently preparing a bee pollen aqueous extract and relates to the technical field of processes for extracting effective materials from bee pollens. In the method, a coarse bee pollen aqueous extract is dissolved after being subjected to low-temperature freezing, because the specific gravity of the bee pollen aqueous extract is greater than that of a water molecule, after a process of standing and layering is carried out, the obtained lower-layer substance is a pure bee pollen aqueous extract in which large numbers of water molecules are removed, and the obtained upper-layer substance is water (containing less bee-pollen aqueous-extracts) which can be used for bee pollen extraction next time. By using the method disclosed by the invention, the concentration of effective active substances in the bee-pollen aqueous-extract is increased by 1-2 times, the extraction cost is reduced by more than 50%, and the biological activities are not affected. The pure bee pollen aqueous extract prepared by using the method disclosed by the invention can be directly prepared into various products with other excipients without being subjected to spray drying.
Description
Technical field
The present invention relates to from bee pollen, extract the Technology field of active substance.
Background technology
Prostate is attached body of gland maximum in the male genetic urinary system, is positioned at the bladder below, and just as Semen Castaneae, the prostatic fluid of its secretion is the important component of seminal fluid.Prostatosis is called " cancer that is not cancer " by medical circle.
Fatty liver generally is because decomposition and the anabolism disequilibrium of fat in the liver or transport the generation obstacle, fat will be in hepatocyte excess accumulation, when fat content increases to more than 30%, then be called fatty liver.The developed area sickness rate is higher, and morbidity has the trend of younger ones in an age groupization, has become the common hidden danger of a serious harm human health.Therefore, the control fatty liver has caused the great attention of medical circle.
Sexual precosity refers to that the child obviously shifts to an earlier date at the sexually matured age, and sexual precosity often follows body development to accelerate, as health increase, muscle development is accelerated, epiphysis is closed in advance, and growth stops earlier.
The women that premature ovarian failure (POF) refers to normal development caused ovarian function failure with front because of certain reason at 40 years old.Except being subjected to congenital heredity factor regulation and control, mature women's ovarian function keep the impact that also is subjected to posteriori many factors, the true cause of its morbidity fully aware of not still up to now is so become focus and the difficult point of domestic and international reproductive development circle to the research of this disease.
The inventor is contained the multiple water-soluble substances that can treat the diseases such as prostate hyperplasia, fatty liver, sexual precosity and premature ovarian failure through for many years systematic study discovery in bee pollen, and realizes that the high efficiency extraction difficulty is very large.Proposed in the past first with bee pollen after the jet mill broken wall treatment, in normal-temperature water immersion, ultrasonic Treatment, then centrifuging and taking gets the bee pollen aqueous solution in High speed refrigerated centrifuge, again with the bee pollen aqueous solution under 1～5 ℃ ambient temperature, cross the 5000D molecular sieve, can only obtain molecular weight and be the thick bee pollen water extract below 5000, wherein contain large quantity of moisture, also need the spray-dried material that could obtain to be added in the goods.
Summary of the invention
The invention reside in invention a kind of efficiently for the preparation of the preparation method for the treatment of the disease bee pollen water extracts such as prostate hyperplasia, fatty liver, sexual precosity and premature ovarian failure.
The present invention includes following steps:
1) with bee pollen through the jet mill broken wall treatment;
2) under the condition of normal pressure, after the normal-temperature water immersion, ultrasonic Treatment;
3) be in 8000～16000 High speed refrigerated centrifuges that turn centrifugal 10～15 minutes at rotating speed, obtain respectively a bee pollen aqueous solution and a solid matter;
4) with the bee pollen aqueous solution, under 1～5 ℃ ambient temperature, cross the 5000D molecular sieve, obtain molecular weight and be the thick bee pollen water extract below 5000;
5) described thick bee pollen water extract after freezing 24～48 hours, is dissolved standing demix at-20～-30 degree under 1～5 ℃ ambient temperature, take off a layer thing, i.e. pure bee pollen water extract.
The present invention with thick bee pollen water extract freezing after again the dissolving, because the proportion of bee pollen water extract is greater than the proportion of hydrone, lower floor's thing of standing demix is the pure bee pollen water extract of removing large quantity of moisture, and topper is and contains the lower water of bee pollen water extract, also can be used as the usefulness of extracting bee pollen next time.Adopt the inventive method, can make that effective active matter concentration improves 1-2 doubly in the bee pollen water extraction liquid, reduce extraction cost more than 50%, and can not affect its biologic activity.The pure bee pollen water extract that obtains through the present invention is can be more spray-dried and directly make various products with other excipient.
The present invention also can take full advantage of the solid matter of first separation, that is: under the normal pressure, with a described solid matter and normal-temperature water with the volume ratio of 1 ︰ 1～4 mix soak after, through ultrasonic Treatment 10～20 minutes, obtain secondary bee pollen aqueous solution with centrifugal separation again, secondary bee pollen aqueous solution under 1～5 ℃ ambient temperature, is crossed the 5000D molecular sieve, obtain molecular weight and be the thick bee pollen water extract below 5000.Solid matter is recycled, effective water soluble ingredient in the solid impurity that remains under for the first time centrifugal is further kept, when extracting bee pollen, use next time, concentration of active substance in can Effective Raise bee pollen water extraction liquid can improve and makes that effective active matter concentration improves 2～4 times in the bee pollen water extraction liquid.
In the step 1) of the present invention, the classification electric machine frequency of stream pulverizer is 55～60Hz, bleed pressure 0.7～1.0Mpa, and the processing time is 0.5～2 hour.Purpose is that the bee pollen conidial cell wall is destroyed, and is convenient to the extraction of active ingredient in the bee pollen and keeps active.
The specific embodiment
One, preparation bee pollen water extract:
1, go-on-go bee pollen (can be tea bee pollen or Brassica campestris L pollen or loose bee pollen or lotus bee pollen or pollen of Semen Fagopyri Esculenti or seed watermelon bee pollen): remove at normal temperatures bee pollen impurity.
2, bee pollen is placed stream carried out low-temperature airflow head-on collision broken wall treatment in the pulverizer 0.5～2 hour, the classification electric machine frequency of control stream pulverizer is 55～60Hz, bleed pressure 0.7～1.0Mpa.
3, under the condition of normal pressure, soak 20～30 minutes with normal-temperature water after, ultrasonic Treatment 20～40 minutes.
4, be in 8000～16000 High speed refrigerated centrifuges that turn centrifugal 10～15 minutes with the mixture after the ultrasonic Treatment at rotating speed, obtain respectively bee pollen water solution A and solid matter B.
5, under the normal pressure, with solid matter B and normal-temperature water with the volume ratio of 1 ︰ 1～4 mix soak after, through ultrasonic Treatment 10～20 minutes, be in 8000～16000 High speed refrigerated centrifuges that turn centrifugal 10～15 minutes at rotating speed again, obtain respectively bee pollen aqueous solution B.
6, merge bee pollen water solution A and bee pollen aqueous solution B, after under-20～-30 ℃ of conditions freezing 24～48 hours, under 1～5 ℃ ambient temperature, dissolve standing demix, take off a layer thing, be pure bee pollen water extract, supernatant is as the usefulness of extracting bee pollen next time.
Two, preparation oral formulations and ejection preparation:
1, preparation oral formulations A:
Pure bee pollen water extract and Mel or other food additive are mixed well, and after ultrafiltration and pasteurization sterilization, fill is for subsequent use.
2, preparation ejection preparation B:
With mixed with the volume ratio of 1 ︰ 1～2 with injecting normal saline after the pure bee pollen water extract pasteurization sterilization, evacuation seals after the uniform dissolution, and is stand-by.
Claims (2)
1. the high efficiency preparation method of bee pollen water extract may further comprise the steps:
1) with bee pollen through the jet mill broken wall treatment;
2) under the condition of normal pressure, after the normal-temperature water immersion, ultrasonic Treatment;
3) be in 8000～16000 High speed refrigerated centrifuges that turn centrifugal 10～15 minutes at rotating speed, obtain respectively a bee pollen aqueous solution and a solid matter;
4) with the bee pollen aqueous solution, under 1～5 ℃ ambient temperature, cross the 5000D molecular sieve, obtain molecular weight and be the thick bee pollen water extract below 5000;
It is characterized in that: described thick bee pollen water extract after freezing 24～48 hours, is dissolved standing demix at-20～-30 degree under 1～5 ℃ ambient temperature, take off a layer thing, i.e. pure bee pollen water extract.
2. the high efficiency preparation method of described bee pollen water extract according to claim 1, it is characterized in that: the classification electric machine frequency of jet mill is 55～60Hz in the described step 1), bleed pressure 0.7～1.0MPa, the processing time is 0.5～2 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102166474A CN102319267B (en) | 2011-08-01 | 2011-08-01 | Method for efficiently preparing bee pollen aqueous extract |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011102166474A CN102319267B (en) | 2011-08-01 | 2011-08-01 | Method for efficiently preparing bee pollen aqueous extract |
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| Publication Number | Publication Date |
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| CN102319267A CN102319267A (en) | 2012-01-18 |
| CN102319267B true CN102319267B (en) | 2013-04-24 |
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| CN2011102166474A Expired - Fee Related CN102319267B (en) | 2011-08-01 | 2011-08-01 | Method for efficiently preparing bee pollen aqueous extract |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102940653A (en) * | 2012-11-30 | 2013-02-27 | 扬州大学 | Bee pollen water extract buccal tablet for preventing and treating alcoholic fatty liver |
| CN103039752A (en) * | 2013-01-11 | 2013-04-17 | 江西旺大动物科技有限公司 | Immunization enhanced type piglet early-stage fodder |
| CN105053769A (en) * | 2015-07-10 | 2015-11-18 | 张彦文 | Preparation method of active pollen honey |
| CN105053768A (en) * | 2015-07-10 | 2015-11-18 | 张彦文 | Preparation method of active pollen nutrient solution |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119939A (en) * | 2010-12-28 | 2011-07-13 | 周斌 | Angiosperm bee pollen water extract for treating fatty liver and preparation method and application thereof |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119939A (en) * | 2010-12-28 | 2011-07-13 | 周斌 | Angiosperm bee pollen water extract for treating fatty liver and preparation method and application thereof |
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| CN102319267A (en) | 2012-01-18 |
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Address after: 225802 Jiangsu city of Yangzhou province Baoying County Jing Town Industrial Zone No. 14 crossing Patentee after: Zhou Bin Patentee after: Ye Manhong Address before: 225001 Jiangsu City, Yangzhou Old City Lane seven 18-3 Patentee before: Zhou Bin Patentee before: Ye Manhong |
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Address after: No. 8, Dashan Industrial Park, Gao Hong Kong, Taizhou, Jiangsu Co-patentee after: Ye Manhong Patentee after: Zhou Bin Address before: 225802 Jiangsu city of Yangzhou province Baoying County Jing Town Industrial Zone No. 14 crossing Co-patentee before: Ye Manhong Patentee before: Zhou Bin |
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Granted publication date: 20130424 Termination date: 20170801 |