CN105524136A - Method for electromagnetic wave nano supercritical extraction of biological active peptides and composite cytokines - Google Patents
Method for electromagnetic wave nano supercritical extraction of biological active peptides and composite cytokines Download PDFInfo
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- CN105524136A CN105524136A CN201510977709.1A CN201510977709A CN105524136A CN 105524136 A CN105524136 A CN 105524136A CN 201510977709 A CN201510977709 A CN 201510977709A CN 105524136 A CN105524136 A CN 105524136A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/24—Extraction; Separation; Purification by electrochemical means
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
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- Proteomics, Peptides & Aminoacids (AREA)
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- Chemical Kinetics & Catalysis (AREA)
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- General Chemical & Material Sciences (AREA)
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- Medicines Containing Plant Substances (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The method for electromagnetic wave nano supercritical extraction of plant active peptides and composite cytokines comprises the following steps: carefully selecting a to-be-extracted plant raw material without deterioration, insect pests and mildew; sending the to-be-extracted plant raw material to a lifting machine through an automatic transmission line, and carrying out rinsing and drying treatment; cutting up the plant raw material after drying treatment, to obtain a coarse-crushed plant powder; sending the plant material to a condenser having a high-frequency high-voltage electronic magnetic field, and carrying out electromagnetic wave nano supercritical extraction formed by means of polarity change, shock wave and tearing at the speed of 245 million times/second; carrying out disinfection and sterilization treatment on the obtained extract; and carrying out vacuum packaging on the extract finished product after disinfection and sterilization treatment. The current plant extraction forefront biological engineering technology is adopted, the plant extract active peptide constant is guaranteed to be about 50 mm, and the plant extract active peptides have active substances and can completely be absorbed by human bodies; the stability is good, the half-life period is prolonged, and the application effect is significant.
Description
Technical field
The present invention relates to technical field of biology, particularly relate to a kind of extracting process extracting plant active peptides and multiple cytokine.
Background technology
Within in 1902, medical college of London University physiologist finds the magical substance polypeptide of stimulating pancreas excreting insulin in animal stomach, and obtains the prize of Nobel's physiology.Polypeptide has and regulates body physiological function and provide nutrition dual-use function to affect all carriers of human body for body.Lacking bioactive peptide in human body can cause metabolism disorder that various disease occurs, and affects life-span length.Plant materials, organism are all containing peptide material.Higher plant (containing herbal medicine) biology of prokaryotes characteristic all has solid cell walls, effective constituent is all wrapped in cell walls, traditional decoction, cream ball, pellet, loose eating method only have 20-30 absorb by tissue, up to now, domestic and international existing abstraction technique proposes containing supercritical carbon dioxide extraction, ultrasonic extraction technology and lytic enzyme the participation that alcohol-extracting technology nearly all needs solvent.Solvent mostly has corrodibility.The peptide chain activity extracted is affected, and the transformation period is short, and real is amino acid whose molecular weight, and the constant of molecular weight, all at (1000-2000mm), not easily passs through epithelial cell membrane and the degraded of resisting hydrochloric acid in gastric juice, digestive ferment.Histocyte is difficult to absorb, and bioavailability is low, is difficult to the polypeptide effectively supplementing human body shortage.There are Three Difficult Issues in the technology of present all extraction bioactive peptides: 1) peptide molecule radix is excessive, poor in (1000-2000mm) microbial film perviousness.2) the polypeptide stability difference transformation period is short, can not resist the degraded of comforter batt lytic enzyme and proteolytic ferment.Scientist's years of researches are explored, and solve this two big world property difficult problem.
Summary of the invention
The object of the invention is the existence for the problems referred to above, a kind of biotechnology adopting plant extraction forefront, the world today is provided, ensure that plant extract molecular peptide constant is about 50, can be absorbed by the body completely, good stability, phase transformation period extends, the method for effect significant hertzian wave nanometer supercritical extraction plant active peptides and multiple cytokine.
The object of the invention is to be achieved through the following technical solutions:
A method for hertzian wave nanometer supercritical extraction plant active peptides and multiple cytokine, is characterized in that comprising the following steps:
(1), utilize electron screening machine to carry out magnetic separation to plant material, choose without deterioration, plant material to be extracted without insect pest, mildew;
(2), by automatic delivery line, plant material to be extracted is delivered to lift and carry out rinsing and drying and processing;
(3), by the plant material after drying and processing pulverize, obtain broken pure plant powder;
(4), by pure plant powder deliver in the condenser with high-frequency high-pressure electromagnetic field and carry out hertzian wave nanometer supercritical extraction, adopt the hertzian wave between 10000 ~ 300,000MHz, freely through extraction pure plant powder, extract at a lower temperature, by filtering separation residue, obtain extract;
(5), high-temperature disinfection treatment is carried out to the extract obtained;
(6), the extract finished product after high-temperature disinfection treatment is packed.
The present invention is that the whole world is pioneering, by electronics magnetic wave nanometer supercritical extraction biologically active peptides and multiple cytokine and all technical, all prior aries are surmounted, achieve peptide molecule constant about 50, barrier cell can be passed through, lytic enzyme and proteolytic enzyme can be resisted to the degraded of peptide activity simultaneously, achieve that effective constituent can be absorbed well by human body, absorbs soon, hypersorption.
Process characteristic of the present invention is as follows:
1. the extract that (nanotechnology) is complete is under the effect in uniform electronic magnetic field, the polarity arrangement of molecule of extract inside, peptide section produces instantaneous polarization under the effect of uniform magnetic field, and do reversal of pole campaign with the speed of 2.45 hundred million times/second, thus produce key vibration, tear phase mutual friction between particle, collision, form nano level small molecule active particle, production specification (i.e. the controllable property of production technique) can be adjusted according to the difference of the Application Areas of product.Its technology is in clear ahead status, is uniquely to have clinical treatment actual value " active polypeptide, organized enzyme and the various active factor " extractive technique at present;
2. under the effect of electronics high temperature, it is external that the crystalline water molecules of extract inside is seeped into extract, and the material such as polypeptidase of liquid form is extracted out with water molecules in the process, and polypeptidase extracting solution product taste is felt all right, water-solublely can to blend by force, substantially without color;
3. extract shape and include composition and be not changed in extraction process, as soybean still can be extracted oil, and improve oil yield 3% ~ 5% on the original basis, soya-bean oil quality index beans cypress quality index not only can not reduce, but also can be improved.
The present invention also has the following advantages:
A, quality are high, output is large, cost low (8 ~ 10 times lower than other extraction costs), product stability are good, and decay of activity is slow;
B, to extract, there is highly selective, active substance variation, can take various plants compound collecting (the method at present in the world any a company all cannot accomplish) simultaneously simultaneously;
C, to save time, the time of 50% ~ 90% can be saved;
D, solvent load few (can comparatively ordinary method few 50% ~ 90%)
E, low power consuming;
F, technology are simple, and production line Costco Wholesale is low;
G, product profit and economic benefit and social benefit huge.
Embodiment
The method of hertzian wave nanometer supercritical extraction plant active peptides of the present invention and multiple cytokine, comprises the following steps:
(1), utilize electron screening machine to carry out magnetic separation to plant material, choose without deterioration, plant material to be extracted without insect pest, mildew;
(2), by automatic delivery line, plant material to be extracted is delivered to lift and carry out rinsing and drying and processing;
(3), by the plant material after drying and processing pulverize, obtain broken pure plant powder;
(4), by pure plant powder deliver in the condenser with high-frequency high-pressure electromagnetic field and carry out hertzian wave nanometer supercritical extraction, adopt the hertzian wave between 10000 ~ 300,000MHz, freely through extraction pure plant powder, extract at a lower temperature, by filtering separation residue, obtain extract;
(5), high-temperature disinfection treatment is carried out to the extract obtained;
(6), the extract finished product after high-temperature disinfection treatment is packed.
The mechanism of technical solution problem of the present invention is as follows:
The mechanism of 1 electron extraction can from the viewpoint of two, and electromagenetic wave radiation process is that high frequency hertzian wave penetrates spe medium on the one hand, arrive inside vascular bundle and glandular cell's system of material.Owing to absorbing electromagnetic energy, cell interior temperature rises rapidly, makes its cell interior pressure exceed cell wall expansion ability to bear, cell rupture.In cell, effective constituent freely flows out, and under lower temperature conditions, spe medium is caught and dissolved.By filtering further and being separated, just obtain extraction material.
2 on the other hand, the electromagnetic field that electromagnetism produces is accelerated to be extracted fractions to extraction solvent interfacial diffusion speed, when using water as solvent, under electromagnetic field, water molecules high-speed rotation becomes excited state, this is a kind of high-energy unsteady state, or water molecules vaporization, strengthens the motivating force of extracted component; Or water molecules itself releases energy and gets back to ground state, the transmission ofenergy discharged gives other material molecules, accelerate its thermal motion, the molecule shortening extracted component is diffused into the time at extraction solvent interface by internal batch, thus make extraction rate improve several times, also reduce extraction temperature simultaneously, ensure quality and the active substance of extraction to greatest extent.
3 simultaneously because the frequency dependence of electromagnetic frequency and molecular rotation connects, so electromagnetic energy is a kind of non-ionising radiation energy being caused molecular motion by ion migration and dipole rotation, when it acts on molecule, facilitate the rotational motion of molecule, if molecule now has certain polarity, just produce under the effect of electromagnetic field and polarize instantaneously, and do reversal of pole campaign with the speed of 2.45 hundred million times/second, thus produce the regular oscillation of key, tear the phase mutual friction between ion, collision, molecular activity part (polar portion) is impelled to contact better and react, generate a large amount of heat energy rapidly simultaneously, impel cell rupture, that enchylema overflows and is diffused in solvent.Reach nano level, possessed penetrate membranes barriers fundamental prerequisite completely, and be controllable (this is the important breakthrough in human history, will bring huge Social benefit and economic benefit to human society) in process of production.
4 molecules are under the effect of high temperature, and the crystalline water molecules of extract inside is seeped into outside extract, and the super various active material such as polypeptidase of liquid form is extracted out with water molecules in the process.Found by microscopic examination simultaneously; bioactive molecule is in leaching process; the grease protective membrane of the outer self-assembling formation even compact of molecule; make it have good biological activity stability; extremely easily long-term (Shanghai, this point current world is unmanned can solve) taste of preserving is felt all right; water-solublely can to blend by force, substantially without color.Efficient active medicaments product can be gone out by extracting directly by adding the different Extraction solvent of adjustment.Extract shape and include composition and be not changed in extraction process, extract raw material is worth unaffected.
Claims (1)
1. a method for hertzian wave nanometer supercritical extraction plant active peptides and multiple cytokine, is characterized in that comprising the following steps:
(1), utilize electron screening machine to carry out magnetic separation to plant material, choose without deterioration, plant material to be extracted without insect pest, mildew;
(2), by automatic delivery line, plant material to be extracted is delivered to lift and carry out rinsing and drying and processing;
(3), by the plant material after drying and processing shred, obtain the plant material of coarse crushing;
(4), pure plant powder is delivered in the condenser with high-frequency high-pressure electromagnetic field and carry out with speed change in polarity, the seismic wave of 2.45 hundred million times/second, tear and form hertzian wave nanometer and supercritical extraction, adopt 10000 ~ 300, hertzian wave between 000MHz, freely through extraction plant material, extract at a lower temperature, by filtering separation residue, obtain extract;
(5), the extract obtained is carried out disinfection, sterilising treatment;
(6), the extract finished product after sterilization, sterilising treatment is packed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510977709.1A CN105524136A (en) | 2015-12-23 | 2015-12-23 | Method for electromagnetic wave nano supercritical extraction of biological active peptides and composite cytokines |
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| CN201510977709.1A CN105524136A (en) | 2015-12-23 | 2015-12-23 | Method for electromagnetic wave nano supercritical extraction of biological active peptides and composite cytokines |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107259627A (en) * | 2017-06-22 | 2017-10-20 | 黄斌华 | Integration of drinking and medicinal herbs nano micromolecule and its preparation technology and integration of drinking and medicinal herbs food |
| CN112704904A (en) * | 2020-12-03 | 2021-04-27 | 百事基材料（青岛）股份有限公司 | Supercritical CO2Method for extracting plant active ingredients by technology |
Citations (3)
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| CN101290277A (en) * | 2007-04-20 | 2008-10-22 | 财团法人食品工业发展研究所 | Rapid extraction method utilizing microwave radiation |
| CN101628016A (en) * | 2009-08-11 | 2010-01-20 | 南昌大学 | Method for extracting active substances from Chinese traditional compound medicine |
| CN102100875A (en) * | 2011-01-28 | 2011-06-22 | 张海 | Process method for microwave assistant supercritical extraction of active ingredients of curcuma |
-
2015
- 2015-12-23 CN CN201510977709.1A patent/CN105524136A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101290277A (en) * | 2007-04-20 | 2008-10-22 | 财团法人食品工业发展研究所 | Rapid extraction method utilizing microwave radiation |
| CN101628016A (en) * | 2009-08-11 | 2010-01-20 | 南昌大学 | Method for extracting active substances from Chinese traditional compound medicine |
| CN102100875A (en) * | 2011-01-28 | 2011-06-22 | 张海 | Process method for microwave assistant supercritical extraction of active ingredients of curcuma |
Non-Patent Citations (4)
| Title |
|---|
| 刘春秀: "植物活性成分的提取技术研究进展", 《安徽农业科学》 * |
| 曹振恒 等: "天然植物有效成分提取分离技术研究进展", 《河北化工》 * |
| 杜方岭: "植物提取物有效成分提取新技术的研究进展", 《农产品加工·学刊》 * |
| 黄忠良 等: "植物功能成分提取技术研究进展", 《河南化工》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107259627A (en) * | 2017-06-22 | 2017-10-20 | 黄斌华 | Integration of drinking and medicinal herbs nano micromolecule and its preparation technology and integration of drinking and medicinal herbs food |
| CN112704904A (en) * | 2020-12-03 | 2021-04-27 | 百事基材料（青岛）股份有限公司 | Supercritical CO2Method for extracting plant active ingredients by technology |
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Application publication date: 20160427 |