KR101158566B1 - beverage manufaturing method mixed of chlorophyll and -glucan - Google Patents

Abstract

The present application does not destroy / damage the effective nutrients of chlorophyll from chlorophyll-containing plants such as mulberry leaves, dandelion leaves, pine needles, thorns, barley, thistle, kelp, persimmon, gompi, etc. A method for producing chlorophyll with economical useful value by safely extracting and concentrating under mild conditions without the use of mild conditions.A plant containing chlorophyll is collected, dried and crushed in a well-ventilated shade to obtain a size of 200 to 300 mesh. A first step of obtaining powder particles; Passing purified water through a ceramic ball or an ocher ball filtration layer to obtain active water obtained by converting a ¹⁷ O nuclear magnetic resonance spectral half width value using an NMR Spectrometer to 70 Hz or less; Mix the active water and ethyl alcohol obtained in the second step, but make a mixed solution in the ratio of active water 3 ± 1: ethyl alcohol 7 ± 1 and inject the plant powder obtained in the first step, low-speed / intermittent stirring conditions, 10 ~ Extracting chlorophyll in a 24-hour range; A fourth step of aging the chlorophyll extract obtained in the third step in a range of 1 to 2 days in a range of 18 ± 2 ° C .; After the aging process of the fourth step is completed, gradually increase the temperature of the concentration tank under vacuum conditions, and first adjust the vacuum degree in the range of 33 ~ 35 ℃ to evaporate ethyl alcohol first, and then gradually increase the temperature of the concentration tank to 38 ~ 40 ℃ range Raising the temperature to remove residual water to obtain a chlorophyll concentrate having a concentration of 80 to 90% chlorophyll; It has the technical idea to obtain chlorophyll through.

Description

Beverage manufaturing method mixed of chlorophyll and β-glucan}

The present application is obtained by extracting chlorophyll as an active ingredient from chlorophyll-containing plants such as mulberry leaves, dandelion leaves, pine needles, thorny stems, countertops (mountain porridge), mistletoe, thistles, kelp, persimmon tea, gompi, etc., mushrooms, mushrooms, chaga It relates to a method for producing a chlorophyll and beta glucan mixed beverage provided by mixing β-glucan extract obtained from mushrooms, such as chlorophyll.

Chlorophyll, also called chlorophyll, is present along with carotene, lutein, and xanthophyll in the chloroplast substrate of the plant's green leaf cells. Chlorophyll has the effect of promoting recovery of skin diseases, burns, and preventing odors. As it turns out, many attempts have been made to process chlorophyll ingredients into beverages, powders or tablets and provide them as pharmaceuticals or health foods.

However, chlorophyll is contained in the chlorophyll grana and binds to the proteins that make up the grana, and it is insoluble in water and soluble in organic solvents such as alcohol ether, benzene and chloroform. In the method of rinsing, the leaves or stems of the plant containing chlorophyll are washed and, if necessary, finely cut and crushed using a mechanical crushing means such as a mixer or a juicer, and the leaves or stems of the crushed plants are pressed. I tried to get the extract by squeezing, but this method had a problem that can not extract a lot of chlorophyll.

In addition, chlorophyll, which is present in the cell walls and cell membranes of plants, has a property that the chemical structure of chlorophyll (chlorophyll nucleus) itself is easy to bind with various substances, so that chlorophyll becomes unstable when extracted from cell walls and cell membranes. Therefore, chlorophyll is likely to cause molecular denaturation due to, for example, oxygen, heat, solvents, etc. in the air, and as a result, derivatives similar to the molecular structure are produced. It is an extremely difficult substance.

In addition, beta glucan (β-glucan), which is mixed with chlorophyll to form a mixed drink, is a polysaccharide present in cell walls, mushrooms, and cereals of yeast and inhibits the proliferation and recurrence of cancer cells by activating the immune function of human normal cells. It is a glucose polymer that decreases blood sugar and blood cholesterol, improves lipid metabolism and suppresses body fat formation and accumulation. It has β-glycosidic linkages at the 1st and 3rd positions, and beta glucan directly attacks cancer cells. By suppressing the proliferation and recurrence of cancer cells by activating the immune function of human normal cells by nonspecific immune response and activating macrophage, they enter the body where cancer cells are and promote the release of various cytokines. It is reported to activate the immune function of immune cells, T cells and B cells. The present application is to provide a chlorophyll component by extracting the chlorophyll as an active ingredient from natural plants, and also by mixing the chlorophyll component obtained by extracting the β-glucan from mushrooms, such as mushrooms, mushrooms, chaga, etc. It is to confirm that the mixed beverage is effective for diabetes / hypertension and to provide a mixed beverage containing chlorophyll and β-glucan.

Chlorophyll is very sensitive to light and heat despite having various beneficial pharmacological effects, and it is easy to destroy nutrients of chlorophyll, and the active ingredient of chlorophyll is not easily dissolved in water. Means have been used.

Looking at the prior art proposed as a means for extracting the active ingredient of chlorophyll, Patent No. 0629927 discloses a raw material mainly composed of silkworms (silk), sonicated and dissolved in volatile ethanol to contain and maintain chlorophyll. An extraction step of extracting the chlorophyll-containing material containing the component and the protein, a separation step of separating the residue from the liquid treated by the extraction step, followed by evaporation of ethanol to separate the chlorophyll-containing material, and chlorophyll obtained in the separation step A method of obtaining a chlorophyll-containing beverage comprising a step of mixing the contents with clean water and performing an ultrasonic emulsification treatment is provided.

However, in the process, the extraction of chlorophyll active ingredient using ethanol, an organic solvent, continuously performs intense sonication and impact, thereby destroying the effective nutrients of chlorophyll, which are extremely sensitive to light or heat, to achieve economic goals. Has had the problem of not being an effective adaptation method.

In addition, in the technique of Patent No. 0811680, a freeze-drying method of mulberry leaves is proposed, by using a freeze-drying method of sublimating ice with gas in a rapidly frozen state of mulberry leaves to prevent the destruction of chlorophyll component of dried mulberry leaves of high quality. As a means for obtaining a chlorophyll raw material, taking the leaves from the mulberry (Morus) and washing with clean water; Natural drying step of removing the moisture by naturally drying the washed mulberry leaves in the shade; Placing the naturally dried mulberry leaves in a flat bottom container; Adding a container containing mulberry leaves to a freezing chamber maintained at a temperature of -35 to -45 ° C to rapidly freeze for 23 to 25 hours; Decompression step of freezing completed mulberry leaf with a container to a freeze drying chamber and put on a cold plate equipped with a multi-stage and reduced pressure for 50 to 70 minutes to maintain 0.3 ~ 0.2 Torr vacuum of the pressure chamber; The heat medium was circulated through the heat dissipation pipe built into the cold plate of the freeze drying chamber, and the hot plate was 9 to 10 hours at 80 ° C, 9 to 10 hours at 70 ° C, 4 to 5 hours at 65 ° C, and 60 ° C. Drying at temperature for 3-4 hours; Stop supply of the heat medium circulating the cold plate of the freeze-drying chamber, and circulate the refrigerant in the cooling pipe built in the cold plate to cool the hot plate 50 ~ 70 minutes while maintaining the temperature of -30 ~ -35 ℃ ; Mulberry leaf lyophilization method comprising the step of taking out the cooled mulberry leaves from the freeze-drying chamber is disclosed, but has a factor of increasing the manufacturing cost due to the complex manufacturing facilities of lyophilization.

In addition, a method for obtaining β-glucan has also been proposed a number of techniques, the method of extracting chaga mushroom Patent No. 0574356 Patent technology discloses a method of obtaining beta glucan with the extract of chaga mushroom extracted by a multi-step extraction method by temperature Bar (a) hot water extract the chaga mushroom at 70 ~ 85 ℃ and filtered to separate the filtrate and the residue; (b) extracting the residue of step (a) from hot water at 80 to 100 ° C. and filtering to separate the filtrate and the residue; And (c) mixing the filtrate of steps (a), (b) and (c) obtained from the step of (b) extracting the hot water at 100-121 ° C. and filtering and separating the filtrate and the residue. An extract of chaga and a technique to provide a health supplement containing the extract is disclosed.

In addition, Patent No. 0734898 discloses a pharmaceutical composition comprising beta-glucan and a pharmaceutically acceptable carrier as an active ingredient for treating kidney disease selected from the group consisting of diabetic nephropathy, acute renal failure and subacute renal failure. The technical idea to apply effectively to a composition is disclosed.

However, in the prior art, the method for obtaining a chlorophyll drink or the partial separation of mushroom extracts for obtaining beta-glucan are mostly used, and when these ingredients are mixed, the pharmacological effect or the effective nutrients are not destroyed. There has not been suggested a mixed drink of chlorophyll and beta-glucan, which is provided as a water-soluble beverage for convenience.

Therefore, the present application obtains chlorophyll by safely extracting / concentrating the chlorophyll active ingredient under mild conditions where the effective nutrients of chlorophyll, which are extremely sensitive to light or heat, are not destroyed, and also mushrooms such as mushrooms, mushrooms and chaga mushrooms. We extracted β-glucan safely from β-glucan to obtain the β-glucan extract and examined the characteristics of the synergistic effect of the mixed beverage mixed with chlorophyll on diabetic disease. It became.

The present application does not destroy / damage the effective nutrients of chlorophyll from chlorophyll-containing plants such as mulberry leaves, dandelion leaves, pine needles, thorns, barley, thistle, kelp, persimmon, gompi, etc. To find a method to obtain water-soluble chlorophyll by safely extracting and concentrating under mild conditions, and to extract β-glucan from mushrooms such as mushrooms, mushrooms, and chaga, It is an invention started with the problem of finding the compounding condition which shows the maximum effect.

The present application extracts / concentrates chlorophyll active ingredients safely under mild conditions in which the active nutrients of chlorophyll, which are extremely sensitive to light or heat, are not destroyed, and also obtains chlorophyll from mushrooms such as mushrooms, mushrooms, and chaga. β-glucan can be safely extracted to obtain β-glucan extract, and chlorophyll and beta-glucan are provided as a water-soluble beverage so that the pharmacological effects and effective nutrients of these mixed ingredients are not destroyed. It has a purpose.

More specifically, the present application extracts the chlorophyll component from the chlorophyll-containing plant by using a mixed solvent of active water and ethyl alcohol, extracts beta glucan from a mushroom using a mixed solvent of active water and ethyl alcohol, and extracts the chlorophyll component and beta glucan. Providing a blended composition, including a concentrated chlorophyll and beta glucan drink provides a dietary supplement to reduce Glucose and CPK, GOT, GPT levels and increase total cholesterol, HDL levels in patients with diabetes It has a purpose.

The present application as a means for achieving the above object, a method for obtaining a water-soluble chlorophyll by extracting and concentrating the chlorophyll component from the chlorophyll-containing plant to an effective concentration, mulberry leaf, dandelion leaf, pine needles, thorny scabies, kitchen countertops containing chlorophyll A first raw material preparation step of collecting chlorophyll-containing plants such as (mountain porridge), mistletoe, thistle, kelp, persimmon, gompi, etc., drying them in a well-ventilated shade, and grinding them into powder particles having a size of 200 to 300 mesh; In addition, a second raw material preparation process is required to dry mushrooms such as mushrooms, mushrooms, and chaga mushrooms in the shade to obtain 150 to 250 mesh particles of powder.

Herein, the prepared raw material uses a mixed solvent of ethyl alcohol and active water in order to safely extract under mild conditions without destroying / damaging the active nutrient, and the active water has a ¹⁷ O nuclear magnetic resonance spectrum. In the case of using the converted water having a half-width lower than 70 Hz as an ethyl alcohol and a mixed solvent, chlorophyll and beta glucan can be obtained as water-soluble. In the second step, clean water is used as a means for obtaining the active water. ball or loess ball passed through the filtration layer value ¹⁷ O-NMR spectral half-width using a NMR Spectrometer requires a second stage process for obtaining the number of active conversion to less than 70Hz.

The ordinary water molecule H ₂ O cannot exist as a single molecule, and is bound to each other by an electric force acting between water molecules to maintain a hydrogen bonding state, and is formed in several groups, which are usually clustered of water. Or, it is called a cluster, and it is decided that water is delicious or tasteless according to the size of the cluster, and when the size of the cluster is small, the water feels better than when the size of the cluster is large. It is provided as active water with various characteristics such as water, and the cluster of water is not kept constant by hydrogen bonding, but usually 1x10 ^-12 (1 peak second) depending on the degree of energy absorption or relaxation. In other words, the reconstruction and dismantling of the water community structure is continued for a short time of 1 trillion seconds. And in the bar, the fact that the water cluster continuous, gatneunga the average large cluster structure gatneunga or small cluster structure is an important factor.

In other words, when the energy is sensitized to water, the nucleus of the water in a stable state absorbs energy and is excited at a high energy level. The phenomenon of absorbing energy is called resonance, and in the resonance phenomenon, the nucleus has a low energy level. While the energy absorbs energy from the state and rises to a high energy level, the water molecules undergo vibration, rotation, stretching or micro brown movement, and the energy from the vibration, rotation, stretching or micro brown movement of these water molecules. The energy absorbed by the nucleus is extremely weak, so the water nucleus in the resonance state is transferred to the vibration, rotation, stretching, or micro-brown movement of water molecules, which is called relaxation of energy. Mitigation is always happening.

The relaxation of energy in water can be visually confirmed by measuring changes in line width using nuclear magnetic resonance or nuclear magnetic resonance spectroscopy (NMR), which is the absorption time of nuclear magnetic resonance spectroscopy (NMR). As a signal to receive energy, a narrow half-width measurement in the NMR spectrum indicates a long relaxation time, a wide half-width measurement indicates a short relaxation time, and a long relaxation time indicates that the movement of water molecules Fast means wider half-width measurements mean slower motion of water molecules.

In general, there may be some deviations depending on the sampling position and the object of water, but for example, the half-width measurement of the ¹⁷ 0 nuclear magnetic resonance spectrum of rainwater is about 119 Hz, the tap water is about 117 Hz, and the minerals of Japan's world's longest village The hot spring water is known to be 79 Hz, and human serum has been reported to represent about 140 Hz. As the hot spring water in the world's longest village, which has a narrow half-width measurement, has a short half-width, the relaxation time is long and the movement of water molecules is actively performed. On the other hand, the hot spring water, which has a small cluster structure and contains a large amount of minerals, can be expected to contribute to long life because the micro-ions, calcium, and magnesium contained in the water molecules are metal ionized to activate the living body. The wide range of tap water, rainwater, and human serum have short half-times, so the relaxation time is short. It is predicted that the movement of water molecules is not active and thus has a large cluster structure, and the trace metals contained in water molecules are not ionized and thus do not give activity to the living body.

In the present application, as a means for dissolving chlorophyll insoluble in pure water, as described above, an attempt was made to dissolve chlorophyll by itself by making active water having a small half-width measurement of the nuclear magnetic resonance spectrum as described above, but it was not dissolved, but a mixed solvent of ethyl alcohol and active water. Was found to be dissolved even under mild conditions, and beta glucan was also well dissolved in a mixed solvent of ethyl alcohol and active water, dissolving only plants containing chlorophyll in a mixed solvent of ethyl alcohol and active water, or mushrooms. The invention was completed by confirming that it is possible to dissolve the bay or to dissolve the plants and mushrooms sequentially containing chlorophyll.

Therefore, the mixture of the active water and ethyl alcohol obtained in the second step, but mixed with active water 5 ± 2: ethyl alcohol 5 ± 2 ratio and injecting the plant powder obtained in the first step, slow / intermittent stirring conditions, 10 The third step of extracting chlorophyll in the range of ~ 24 hours is carried out, the mixed solvent composition ratio of ethyl alcohol and active water to extract the active ingredient is from 1: 1 to 1: 1 by volume ratio of ethyl alcohol: active water. Although the mixing ratio up to 9 can be used, in the separation extraction process for extracting only chlorophyll from the mixed solvent through experiments with numerous trial errors, the volume ratio of ethyl alcohol and active water was the highest at 7: 3. In the separation extraction process to obtain only beta glucan, the volume ratio of ethyl alcohol and active water was the highest at 6: 4.

However, in the case of extracting chlorophyll-containing plants and mushrooms from the mixed solvent in one reactor, the volume ratio of ethyl alcohol and active water is 5: 5 or the volume ratio of ethyl alcohol and active water is 6: 4, 7: 3, 8: 2. A close extraction effect was obtained even at the mixing ratio.

Chlorophyll and beta glucan extract obtained through the extraction process of the third step is carried out a fourth step process of stabilizing and aging the chlorophyll active ingredient under mild conditions in the range of 1 ~ 2 days in 18 ± 2 ℃ range.

After the aging process of the fourth step is completed, gradually increase the temperature of the concentration tank under vacuum conditions, and first adjust the vacuum degree in the range of 33 ~ 35 ℃ to evaporate ethyl alcohol first, and then gradually increase the temperature of the concentration tank to 38 ~ 40 ℃ range When the fifth step is performed to remove the residual water by raising the temperature to obtain a mixed concentrate of chlorophyll and beta glucan, the nutrients of chlorophyll and beta glucan are safely removed under mild conditions without destroying / damaging the nutrients. By extracting and concentrating, it is possible to obtain a mixed beverage of chlorophyll and beta glucan having a high economic value.

The vacuum concentration condition is a process for safely removing alcohol or water at a temperature of less than 40 ℃ because chlorophyll is deteriorated by heat at a temperature of 45 ℃ or more, or the bonding structure is broken, the present application is chlorophyll in the same manner as described above Extract the chlorophyll component from the containing plant using a mixed solvent of active water and ethyl alcohol, extract beta glucan using a mixed solvent of active water and ethyl alcohol from mushrooms to obtain a mixed composition containing the chlorophyll component and beta glucan, The chlorophyll and beta glucan mixed drink provided by concentrating it can provide a health supplement provided to benefit patients with diabetes.

The present invention provides effective nutrition of chlorophyll under mild conditions from chlorophyll-containing plants such as mulberry leaves, dandelion leaves, pine needles, spiny ginseng, countertops, thistles, thistle, kelp, persimmon, gompi, etc. Chlorophyll is safely extracted and concentrated in a state in which the ingredients are not destroyed / damaged to obtain stabilized chlorophyll, and beta glucan is extracted using mushrooms selected from situation mushrooms, matsutake mushrooms and chaga mushrooms, which are provided as a mixed component thereof. Has the effect of providing a drink.

Chlorophyll and beta glucan beverages provided herein have the effect of reducing blood levels of Glucose, CPK, GOT, GPT, and increasing total cholesterol and HDL levels in patients with diabetes mellitus and providing them as health supplements. .

In addition, chlorophyll has been developed as a dietary supplement or medicine for gastrointestinal diseases, diabetes, acne, blemishes, freckles, chronic anemia, high blood pressure, stroke, hepatitis, cirrhosis, etc. It has the effect of providing a method for extracting chlorophyll so that it can be used.

1 is a process for producing a chlorophyll as a target from a plant containing chlorophyll as a first extraction step, and obtaining beta glucan as a target from a mushroom at a second extraction step, and mixing and concentrating them to obtain a mixed concentrate.
Figure 2a: Unit activated water device overview for obtaining active water used to extract chlorophyll in the present application.
FIG. 2B is a schematic view of an active water group having a configuration in which a plurality of unit active water groups of FIG. 2A are connected; FIG.
Figure 3: Overview of the device is a vacuum concentration in the concentration tank of the present application.
4a to 4f: Experimental data for determining the correlation with the diabetes-causing factor using the mixed concentrate of the present application.

Prior to presenting the embodiments of the invention for realizing the technical idea of the present application in the drawings and examples, the terms or words used in the specification or claims of the present application should not be construed as being limited to ordinary or dictionary meanings. The scope of the present application should be interpreted as meanings and concepts consistent with the technical spirit of the present invention, and the embodiments shown in the specification or the drawings are merely one preferred application description and represent all of the technical ideas of the present application. It is to be understood that there may be various equivalents and variations in place of them at the time of the present application.

In addition, the embodiments of the drawings presented below in the technical idea of the present application to one application for implementing the technical idea of the present application, the facility or control system used in the existing food or beverage processing apparatus in the configuration presented herein Of course, this can be applied in addition.

Hereinafter, in order to easily understand the technical idea of the present invention, in FIG. 1, a first extraction step of obtaining chlorophyll from a chlorophyll-containing plant and a second extraction step of obtaining beta glucan from mushrooms are separately performed, and in a mixed aging tank. And beta glucan are mixed and aged, and through the concentration tank, chlorophyll and beta glucan are presenting a manufacturing process for obtaining a mixed drink, Figure 2a is a unit activated water device to obtain the active water used to extract chlorophyll in the present application 2b shows a device for obtaining active water by connecting a plurality of unit active water groups of FIG. 2a in parallel, and FIG. 3 shows a concentration tank for separating chlorophyll and betaglucan mixed solution from alcohol and water under vacuum conditions. An example is shown.

Figure 1 is a plant that contains chlorophyll-containing plant chlorophyll containing chlorophyll, dandelion leaf, pine needles, thorny stems, countertops (mountain porridge), mistletoe, thistle, kelp, persimmon, gompi, gompi, etc. Dry in this well-known shade, crushed into 200 ~ 300 mesh size powder particles and stored in the raw material storage tank (10a), ethyl alcohol in the ethanol storage tank (20a) and active water storage tank (30a ') to the extractor having a stirrer 7 ± 1: Make a mixed solution in the extraction reaction tank (40a) with the active water 3 ± 1 ratio, and slowly stir in the clockwise direction at the speed of 100 ~ 120rpm. Injected, stirred, and dissolved, the amount of solvent used in ethyl alcohol and active water may be used in an amount of 5 to 10 times the solute weight of the chlorophyll-containing plant grind.

Another raw material storage tank (10b) is pulverized mushrooms, mushrooms, mushrooms, mushrooms selected from chaga mushrooms into particles of 150 ~ 250 mesh size, into the extractor having an agitator, the alcohol alcohol storage tank (20b) and the active water storage tank (30b) ') In the ethyl alcohol 6 ± 1: active water 4 ± 1 ratio into the extraction reaction tank (40b) to make a mixed solution, while slowly stirring in a clockwise direction at 100 ~ 120 rpm speed mushroom grinding in the raw material storage tank (10b) A small amount of water can be injected and stirred to dissolve it.

The active water used in the extraction process of the present invention uses weak alkaline water provided with a half-width measurement of the nuclear magnetic resonance spectrum of less than 70 Hz and a pH of 7.5 to 8.5, and it did not dissolve chlorophyll by itself. Chlorophyll or beta glucan can be confirmed to be dissolved in a mild solution of alcohol and active water 5: 5. Preferably, the active water and ethyl alcohol are mixed, but the active water 4 ± 2: ethyl alcohol 6 It can also be applied as a method of preparing a mixed solution at a rate of ± 2 and injecting chlorophyll-containing plant powder and mushrooms in a single extraction reactor one by one in a slow / intermittent stirring condition, in the range of 10 to 24 hours.

In addition, after the extraction process is completed, gradually increase the temperature of the extraction reaction tank 40, which is also combined with a vacuum evaporator, first adjust the vacuum degree at a temperature below 40 ℃ to evaporate about 70 ~ 80% of the amount of ethyl alcohol first 1 After diminishing to about 2, the contents are sent to a centrifugal injector (50) to separate the residue, and the filtrate is sent to a maturation tank (60) to mature in a range of about 1 to 2 days in a range of about 18 ± 2 ℃ and stabilize the chlorophyll. It is preferred that the process is carried out.

At this time, the chlorophyll and betaglucan components in most chlorophyll-containing plant raw materials are determined to be dissolved and extracted in a mixed solvent of ethyl alcohol and active water, but residues other than chlorophyll remain depending on the characteristics of the plant raw materials used. It is a process for separation.

After completion of the aging process is sent to the concentration tank 70 in a vacuum condition to gradually increase the temperature first to adjust the degree of vacuum in the 33 ~ 35 ℃ range to evaporate the ethyl alcohol first, then gradually increase the temperature of the concentration tank 38 ~ 40 ℃ range By increasing the temperature to remove the remaining water to obtain a chlorophyll concentrate having a concentration of chlorophyll 80 ~ 90%.

Figure 2 shows an application of the device for obtaining the active water used to extract the chlorophyll in the present application, the active water production apparatus shown in Figure 2 is invented by Professor Baek Woo-hyun, one of the inventors of the present invention and patent application separately from the present application As an invention, the mixture of ocher powder 70 ± 15w%, oyster shell small powder 20 ± 10w%, sawdust 10 ± 5w% is added to the composition of water with a moderate viscosity while adding a suitable amount of water to a size of about 5 to 20 mm in diameter. It is naturally dried by molding into a ball shape, and the molded ball is first dried / fired at 450 ° C.-550 ° C. for 10-12 hours, and the first calcined ocher ball is secondarily baked at 1000 ° C.-1300 ° C. as a technique using loess ball obtained, far-infrared radiation characteristic in the characteristic evaluation of the loess ball was able to obtain the results shown in Table 1, Table 2 was obtained by pH measurements, ¹⁷ O property test results using NMR Spectrometer Table 3 were obtained.

Name of sample Emissivity
(5-20 μm) Radiant energy
(w / ㎡㎛) Test Methods Remarks
Ocher ball
0.918 3.70 x 10 ² KICM
-FIR-1005 Center wavelength
9.3 μm

sample pH value measuring equipment tap water 7.02 TOA Electronic
HM-149 pH meter  Ocher ball pass 8.01

time
sample 19 minutes 50 seconds 39 minutes 4 seconds measuring equipment tap water 105.4490 Hz 105.3360 Hz Varian's Unit Plus
300NMR Spectrometer  Ocher ball pass 52.1261 Hz 53.9217 Hz

The present application obtains the active water in which the half width measurement of the ¹⁷ 0 nuclear magnetic resonance spectrum is provided below 70 Hz using the ocher ball having the above physical properties, and dissolves chlorophyll and beta glucan by using a mixed solvent mixed with ethyl alcohol. As described above, one application example of an apparatus for producing active water applicable to the present invention will be described with reference to FIG. 2. FIG. 2A shows a unit active water group, and FIG. 2B shows a plurality of unit active water groups connected in parallel. It is a device for obtaining the active water of the desired concentration.

Figure 2a is intended to show an embodiment in which the active water group 30 available herein can be provided in an upflow flow structure in a simple configuration having two ocher ball media layers in the upper housing and a filter section in the lower housing. It may be provided in a simple configuration having the ocher ball filter layer 1 (31) and the ocher ball filter layer 2 (32) at the top and the filter unit 33 at the bottom, and the purified water as the active water inlet 34 (淨) Water is introduced and foreign matter or suspended matter is first filtered through the filter unit 33, and then the water passing through the ocher ball filter layer 2 (32) is guided to flow through the ocher ball filter layer 1 (31) again and the inflow water is yellow soil. Staying in the ball bed for a predetermined time or more may be provided as a system that is converted into active water and discharged through the upper outlet 35, the filter unit 33 provided at the bottom to function to block the inflow of suspended matter, the central portion Furnace blockage structure (33a), It is dispersed to the side and does not flow to the side to form a structure that is filtered through the filter medium (33b), and in order to increase the durability of the filter medium (23b) has a porous stainless steel plate wick (33c) in the center of the filter medium and the upper and lower outer surfaces of the wick portion The filter material 33b may be provided in a structure wrapped, and the inflow water passing through the filter part 33 passes through the ocher ball filter layer 2 (32), and the ocher ball filter layer 2 (32) is the filter support portion 32a. Water flows into the central part of the inlet is guided to flow through the plurality of partitions (32b) filled with ocher balls, the plurality of partitions (32b) is one of the upper passageway (32c), the other side of the lower passageway It is provided to have (32d) alternately and the ocher ball is filled between the partition walls so that the introduced water does not flow through only one ocher ball, but the ocher ball media layer 1 (31) through the ocher balls filled between the entire partition walls. Will be sent to In order to induce the flow in the zigzag form, the first layer 31 may be blocked by the center of the media support part 31a, and the inflow water may enter the outside of the outer partition 32b to pass the zigzag flow through the plurality of partition walls 31b filled with ocher balls. achieve the partition wall (31b) of this part, in part, is provided so as to have a lower cylindrical sewer (31d) of the inlet water to the upper barrel sewer (31c), the other side to one side is sufficiently in contact with the loess ball ¹⁷ 0 nuclear magnetic half-width of the resonance spectrum value Converted into active water provided in the range of less than 70 Hz can be provided as a unit active water to be discharged through the upper outlet 35 through the central discharge hole (31e), the ocher ball media layer 1 constituting the upper housing (31) ) Or the lower housing surrounding the ocher ball media layer 2 (32) and the pre-filter (33) is a watertight structure with an O-ring (36) or other sealing material to fasten the bolting (37) or a male and female coupling between the upper and lower housings. It is preferable to provide a configuration having a jaw so that the upper housing and the lower housing can be easily separated if necessary so that the media can be exchanged or cleaned. The drain for discharging tailings that do not pass through the filter layer to one side of the lower housing is provided. An application in which the unit active water group can be provided in the configuration having the portion 36 is shown.

FIG. 2B illustrates an example in which the unit active water group of FIG. 2A may be installed in a plurality of parallel structures. The half width of the spectrum provided as a standard for converting the active water into active water here is the size capacity and water supply flow rate of the manufacturing apparatus. For example, when the half width value of the ¹⁷ 0 nuclear magnetic resonance spectrum is designed to obtain 200 L of active water per day in the 70 Hz range using the Jijangsoo manufacturing apparatus of the present application, FIG. The purpose of the present application can be achieved by making a plurality of unit active water groups having the same size as the unit active water group having an inner diameter of 30 cm and the ocher ball having a filling height of 60 cm and connecting them in parallel to each other. The half-width of the spectrum of the water which passed through the 1st manufacturing apparatus 30a was 88 Hz, The half-width of the spectrum of the water which passed through the 2nd manufacturing apparatus 30b was 78 Hz, The half-width of the spectrum of water which passed the 3rd manufacturing apparatus 30c was 72 Hz, 4th An experimental example in which the half-width of the spectrum of the water passed through the roughening device 30d can be provided at about 68 Hz is shown.

Figure 3 is intended to show an embodiment of the operation of the thickening tank 70 used to obtain a high concentration of the concentrate after the extraction / aging of chlorophyll and beta glucan herein, the thickening tank 70 has an elliptical structure of the bottom bottom surface In order to slowly stir the contents of the body 71 and the concentration tank, it has a motor 73 with a reducer and a stirring blade, but the stirring blade has a lower stirring blade 72a and an intermediate stirring blade provided to have a curved structure along the bottom surface ( 72b) has a double jacket portion 74 which forms a heating part by wrapping the lower body 71 of the thickening tank, and the double jacket is provided with a hot water coil or a hot plate built therein to heat the thickening tank, and gradually increases the temperature of the thickening tank. Ethyl alcohol is first evaporated by adjusting the degree of vacuum in the range of ~ 35 ℃, then gradually increasing the temperature of the concentration tank to remove the remaining water by raising the temperature to the range of 38 ~ 40 ℃. The chlorophyll can be concentrated to a desired concentration. Referring to FIG. 4, the process of vacuum distillation is carried out. The vacuum pump 81 is used to lower the pressure inside the concentration tank 70 to a low vacuum state and the contents of the concentration tank. When the temperature is gradually increased, first, the degree of vacuum is controlled in the range of 33 to 35 ° C. to evaporate the ethyl alcohol component first, and the evaporated alcohol component is cooled and liquefied by the condenser 82, and the ethyl alcohol distillate is stored in the first tank 83. First, gradually increase the temperature of the contents of the evaporated alcohol component to increase the temperature to 38 ~ 40 ℃ range to control the vacuum degree, the remaining alcohol component and water is distilled and cooled and liquefied by the condenser 82, the second tank The first tank 83 and the second tank 84 may be first stored in the first tank 83 through the valve of the pipe connected to the condenser 82 at the top of each tank. 83) the top valve opens The upper valve of the second tank 84 is closed so that the alcohol liquid is stored only in the first tank 83. When the initial alcohol distillation is finished, the upper valve of the first tank 83 is closed and the second tank 84 is closed. The valve at the top opens to show that the remaining amount of alcohol and water can be applied to the system where it is stored in the second tank 84. The alcohol in the first tank is reused, and the alcohol and water in the second tank are It is an application example showing that it may be used or discarded for other purposes via the purification filter 85 and the second purification filter 86.

In the extraction reaction tank 40 of the present application, the vacuum distillation system of FIG. 4 is applied as it is even when the method of first raising the temperature to 40 ° C. or lower and adjusting the vacuum degree to first evaporate about 70 to 80% of the ethyl alcohol usage. Of course, it can be reduced to about 1/2.

As an example of application of the technical idea of the present invention, chlorophyll-containing plant material is mulberry leaf powder 40 ± 15 w%, pine needle powder 10 ± 5 w%, mountain bamboo powder 10 ± 5 w%, mistletoe 10 ± 5 w%, thistle powder 10 ± 5 w% jujube in 2 ± 1 w% order to dissolve the extract to obtain the extract, chlorophyll and beta glucan extract by injecting 20 ± 5 w% of the mushroom powder selected from the situation mushroom, matsutake mushroom, chaga mushroom To obtain a mixed drink as a bar, extraction / concentration at the similar composition ratio as described above to obtain a mixed drink sample, the sample drink was to measure the correlation with diabetes.

Mixed Beverage Preparation Example

In a reactor equipped with a double-jacket structure having a vacuum concentration function on a 200-l scale, first, a mixed solvent of 50 l of active water and 80 l of ethyl alcohol having a width of 68 Hz of ¹⁷ 0 nuclear magnetic resonance spectrum was prepared. Add 8 kg of mulberry leaf powder, 2 kg of pine needle powder, 2 kg of mountain bamboo powder, 2 kg of mistletoe, 2 kg of thistle powder, 400 g of jujube powder in order, and stir for about 6 hours to extract / dissolve it. After adding 4 kg and stirring the mixture for 30 minutes and stopping for 30 minutes, the extraction process was carried out. After about 10 hours, the temperature of the reaction vessel was raised to 40 ° C. using the system structure shown in FIG. Separation was carried out by distillation, and the chlorophyll and betaglucan mixed extracts were aged for 24 hours in the 18 ° C range.

After the process is completed, gradually increase the temperature of the concentration tank under vacuum conditions and first adjust the vacuum degree at 35 ° C. to evaporate the remaining amount of ethyl alcohol first, and then gradually increase the temperature of the concentration tank to increase the temperature to 40 ° C. and adjust the vacuum degree. Water was removed to obtain a mixed concentrate of chlorophyll and beta glucan.

In order to examine the correlation with the diabetes-causing factor with respect to the mixed concentrate obtained in the above example, a rat administered the solution (STZ + Chol) diluted twice the mixed concentrate of the present application to the diabetic rat (2) having diabetes. The normal rats were fed normal water (Normal water) and diabetic rats (1) by administering normal water (STZ + water) supplied to normal rats. We measured cholesterol, blood GOT, blood GPT, blood HDL, and blood CPK.

1) Blood Glucose Test

Serum glucose is the average value of 70 ~ 130mg / dl in normal rats, but when diabetes occurs, blood or urine glucose concentration rises rapidly and is excreted from the body and released from the body, blood glucose is the source of metabolic energy As it does not enter the liver, muscle or fat cells, the rise of glucose in the blood is immediately excreted in urine.

In the experiments of the present invention, the normal rats in each treatment group were diluted twice with normal water, diabetic rat (1) + normal water (STZ + water), diabetic rat (2) + mixed concentrate of the present application ( STZ + Chol) was administered in a manner of administering 15 dogs each. As a result of continuous administration of the diluted solution of the double concentration of the present mixture in rat (2) group that induced diabetes, the blood was almost close to normal rats. The glucose concentration was improved, and in the rat (1) group who induced diabetes, the weight loss phenomenon that caused life-threatening weight loss occurred at the same time as the blood glucose concentration lasted more than 400mg / dl. appear. As a result of this, the administration of the mixed concentrate of the present application to the blood-absorbed glucose in the body to the diabetes-induced rats is determined to be effective as an antidiabetic treatment bar, the experimental results are shown in Figure 4a.

2) Total cholesterol test in blood

Serum total cholesterol (TC) is the average value of 90-170 mg / dl in normal rats, but when diabetes develops, total cholesterol in blood rapidly decreases, including free fatty acid, which is a source of metabolic energy introduced from outside the body. Cholesterol is reported to drop sharply, resulting in weight loss due to a decrease in total cholesterol in the blood.

In this experiment, normal rats + normal diet, diabetic rat (1) + general diet, diabetic rat (2) + administration of 2 times diluted solution of the mixed concentrate of the present invention was administered to each of the 15 groups in each treatment group As a result of continuous administration of the mixed concentration dilution of the present in rats (2) induced by, the total cholesterol concentration in blood was improved almost close to that of normal rats, and in the diabetic rats (1), As a result of the total cholesterol concentration of 60 mg / dl or less, the administration of the mixed concentrate of the present application gives the diabetic patient the activity to accumulate and absorb the blood total cholesterol in the body in rats with diabetes. In this regard, the effect as a treatment improving agent is considered to be high, and the experimental results are shown in FIG. 4B.

3) Blood GOT Test

Glutamic oxaloacetic transminase (GOT) is 80-150U / dl in normal rats, but the level of glutamic oxaloacetic transminase increases rapidly in diabetic rats. The specific destruction of β-cells has been reported to increase GOT due to endocrine-induced immunosuppression. GOT levels have been widely used as indicators of disease infection or vitality of organs in normal animals and humans.

In this experiment, normal rats + normal diet, diabetic rat (1) + general diet, diabetic rat (2) + administration of 2 times diluted solution of the mixed concentrate of the present invention was administered to each of the 15 groups in each treatment group As a result of continuous administration of the mixed concentration dilution of the present in rats (2) induced by, the blood GOT concentration was improved almost close to that of normal rats, and in the diabetic rats (1), From the results of blood GOT concentration of 400 Um / dl or more, the administration of the mixed solution of the present invention can reduce the blood GOT level in diabetic rats. This effect is a secondary disease and disease infection that is common in diabetics. It is expected to be highly available as a functional substance that can enhance the immune-endocrine function that can prevent and the experimental results are shown in Figure 4c. .

4) Blood GPT measurement experiment

Glutamic pyruvic transminase (GPT) in normal rats has an average value of 60-100 U / dl, but it is known that the level of glutamic pyruvic transminase rises rapidly in diabetic patients. It is reported that GPT rises by increasing GOT, which is a precursor of GPT, due to an endocrine-immune hypoplasia by specifically destroying β-cells in the pancreas.

In this experiment, normal rats + normal diet, diabetic rat (1) + general diet, diabetic rat (2) + administration of 2 times diluted solution of the mixed concentrate of the present invention was administered to each of the 15 groups in each treatment group Continuous administration of the mixed diluent of the present invention in rats (2) induced by the rats resulted in an improvement in blood GPT levels close to that of normal rats, whereas life was increased in the diabetic rats (1). As a result of blood GPT concentrations of more than 300 Um / dl, which is almost difficult to maintain, the mixed concentration of the present treatment can reduce GPT concentrations and reduce GPT concentrations in rats with diabetes. It is considered to be present and the experimental results are shown in Figure 4d.

5) HDL measurement experiment

Serum high-density lipoprotein (HDL) was found to be 40-70 mg / dl in normal rats. However, in rats induced with diabetes mellitus by STZ in this experiment, blood HDL levels rapidly decreased, resulting in total metabolism. It has been reported that HDL in blood, including cholesterol, is rapidly reduced, resulting in weight loss due to a decrease in HDL concentration in blood. This is a common symptom of elevated blood glucose and total cholesterol in diabetics. It is a phenomenon.

In this experiment, 15 rats were placed in each treatment group in a manner of administering a normal rat + normal diet, diabetic rat (1) + general diet, diabetic rat (2) + a double diluted solution of the mixed concentrate of the present application, Consistent administration of the mixed concentrate dilution of the present in the diabetic rats (2) resulted in an improvement in the blood HDL concentration close to that of the normal rat, whereas in the diabetic rats, the normal HDL concentration As a result of 20 mg / dl or less, the administration of the mixed concentrate of the present invention increased the blood HDL and total cholesterol concentrations in the diabetic rats. Is judged to be high and the experimental results are shown in Figure 4e.

6) Blood CPK test

In normal rats, creatine phosphokinase (CPK) shows 100-180 U / dl. However, when diabetes develops, blood CPK levels rapidly increase. In general, diabetic patients have insufficient insulin secretion and renal function simultaneously. In the case of renal insufficiency, CPK is essentially increased in blood concentration. In this experiment, GOT and GPT abnormalities and CPK were increased due to imbalance of immune endocrine system.

In this experiment, normal rats + normal diet, diabetic rat (1) + general diet, diabetic rat (2) + administration of 2 times diluted solution of the mixed concentrate of the present invention was administered to each of the 15 groups in each treatment group As a result of continuous administration of the mixed dilution of the present in rats (2), which resulted in an increase in the level of blood, GOT and GPT concentrations were improved, and renal function was improved. In the group, the concentration of CPK of 200 Um / dl or less was almost difficult to maintain in life. Therefore, diabetic-induced immune endocrine system abnormalities occurred in rats with diabetes mellitus administration. May reduce blood CPK levels and reduce GOT and GPT levels simultaneously. These results suggest that kidney function is common in diabetics. A substance that can function to improve the symptoms in people with diabetes are considered high availability as a therapeutic agent for improving test results are shown in Fig. 4f.

From the above results, the mixed concentrate of the present application showed a significant decrease in Glucose, CPK, GOT, GPT, etc. in the animal group, and the increase in total cholesterol, HDL, etc. can be confirmed. It is expected to be the basis that can be usefully applied to beverages.

10a: plant material containing chlorophyll 10b: mushroom material
20: ethyl alcohol storage tank 30: active water group
31: ocher ball filter layer 1 32: ocher ball filter layer 2
33: filter portion 34: inlet
35 upper outlet 36 drain portion
40: extractor 50: centrifuge
60: aging tank 70: thickening tank
71: concentration tank inner tank 72: stirring blade
73: motor 74: double jacket
80: enrichment facility auxiliary equipment 81: vacuum pump
82 condenser 83 first tank
84: second tank 85: first purification filter
86: second purification filter 100: chlorophyll and beta glucan mixed concentrate

Claims (6)

In the manufacturing method of extracting the chlorophyll component from the chlorophyll-containing plant, extracting beta glucan from the mushrooms to obtain a mixed composition, and concentrating it to obtain a mixed chlorophyll and beta glucan beverage,
Selecting and harvesting chlorophyll-containing plants and mushrooms, drying and pulverizing in airy shades to obtain powder particles having a size of 150 to 300 mesh;
Passing purified water through a ceramic ball or an ocher ball filtration layer to obtain active water obtained by converting a ¹⁷ O nuclear magnetic resonance spectral half width value using an NMR Spectrometer to 70 Hz or less;
Mix the active water and ethyl alcohol obtained in the second step, but make a mixed solution in the ratio of active water 4 ± 2: ethyl alcohol 6 ± 2 ratio and injecting the plant powder and mushroom powder obtained in the first step low-speed / intermittent stirring conditions A third step of extracting chlorophyll and beta glucan in a range of 10 to 24 hours;
A fourth step of aging the chlorophyll and beta glucan extract obtained in the third step in the range of 18 ± 2 ° C. for 1 to 2 days;
After the aging process of the fourth step is completed, gradually increase the temperature of the concentration tank under vacuum conditions, and first adjust the vacuum degree in the range of 33 ~ 35 ℃ to evaporate ethyl alcohol first, and then gradually increase the temperature of the concentration tank to 38 ~ 40 ℃ range Raising the temperature to remove residual water to obtain a mixed concentrate of chlorophyll and beta glucan;
Chlorophyll (chlorophyll) and beta glucan mixed beverage production method characterized in that comprises a. The method of claim 1,
The chlorophyll-containing plant used in the first step is chlorophyll, characterized in that one or more selected from mulberry leaves, dandelion leaves, pine needles, thorny stems, countertops (mountain porridge), mistletoe, thistle, kelp, persimmon, gompi Method for preparing beta glucan mixed beverage. The method of claim 1,
The mushrooms used in the first step is a method for producing a chlorophyll and beta glucan mixed drink, characterized in that at least one selected from the situation mushrooms, matsutake mushrooms, chaga mushrooms. The method of claim 1,
Chlorophyll is extracted with the mixed solution of the active water and ethyl alcohol obtained in the second step, and then the vacuum degree is controlled in the extraction reactor 40 to evaporate the ethyl alcohol component first, followed by a fourth step process. And a method of preparing beta glucan mixed beverage. The method of claim 1,
Mulberry leaf powder 40 ± 15 w%, pine needle powder 10 ± 5 w%, mountain bamboo powder 10 ± 5 w%, mistletoe 10 ± 5 w%, thistle powder 10 ± 5 w% Jujube powder is added by dissolving in order of 2 ± 1 w% to obtain an extract, and by injecting 20 ± 5 w% of mushroom powder selected from situation mushrooms, matsutake mushrooms and chaga mushrooms, chlorophyll and beta glucan are provided. Method for producing a chlorophyll and beta glucan mixed beverage, characterized in that. delete

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