CN1733055A - Fenugreek seed extract and its preparing process and application - Google Patents
Fenugreek seed extract and its preparing process and application Download PDFInfo
- Publication number
- CN1733055A CN1733055A CNA2005100170766A CN200510017076A CN1733055A CN 1733055 A CN1733055 A CN 1733055A CN A2005100170766 A CNA2005100170766 A CN A2005100170766A CN 200510017076 A CN200510017076 A CN 200510017076A CN 1733055 A CN1733055 A CN 1733055A
- Authority
- CN
- China
- Prior art keywords
- semen trigonellae
- extract
- diabetes
- trigonellae extract
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to an extract of fenugreek seeds, its preparing process and use thereof, wherein the extract contains 60-80 wt% of polysaccharides. Its preparing process comprises, making water boiled liquid of fenugreek seeds through grilling, charging ethanol for deposition and separation, discarding supernatant fluid through centrifugation, removing ethanol from the precipitate with vacuum distillation method, finally drying to obtain the extract of fenugreek seeds, which can be used for preparing the medicament for treating diabetes and diabetic nephropathy.
Description
Technical field
The present invention relates to a kind of Semen Trigonellae extract that from Semen Trigonellae, extracts and preparation method thereof; With this extract is the thick compound of medicine of bioactive ingredients, and their application in treatment diabetes and diabetic nephropathy drugs; This extract has blood sugar regulation in preparation, regulates the application in the health food of protecting renal function and structure function.
Background technology
A kind of important commonly encountered diseases and frequently-occurring disease have been become in China's diabetes.Along with the obvious rising of onset diabetes rate, the incidence of chronic complicating diseases of diabetes is quite outstanding.Diabetic nephropathy is the common chronic complicating diseases of diabetes, is respectively 30～40% and 15～20% in 1 type and its sickness rate of type 2 diabetes mellitus, has become the main cause that threatens the diabetics life and health.The diabetes nephropathy will cause the grievous injury of renal function and structure, finally cause renal failure and threaten patient's life.Estimation diabetic nephropathy in 20 years of future will become the most important reason of end stage renal failure.Therefore, the method and the active drug of research and development control diabetic nephropathy are extremely urgent.
Semen Trigonellae is the dry mature seed (fenugreek seeds) of pulse family annual herb plant Semen Trigonellae (Trigonella foenum-graecum L.), is commonly called as Trigonella foenum-graecum, Rhizoma et radix valerianae etc.At the several province and municipalities of China plantation is arranged at present.Semen Trigonellae is one of China's Chinese medicine material, is recorded by Pharmacopoeia of People's Republic of China (version in 2000).Semen Trigonellae bitter in the mouth, warm in nature, nontoxic has the kidney warming sun, dispelling cold and dampness, analgesic effect, is mainly used in the kidney cold caused by deficiency, cold and pain in the lower abdomen, inguinal hernia, diseases such as the disturbance of lower legs due to pathogenic cold and dampness.Semen Trigonellae is the dietotherapeutic plant, and normal its fruit of using is as flavoring agent between its people such as India.Shan sweet one is with and is among the peoplely then put into wheaten food with the seed powder, controls children's and eats disease etc.Be used for the making of coffee, curry, essence etc. the food industry Semen Trigonellae.Can be made into combination of fenugreek gum with the fenugreek seed endosperm, expand after the water logging, have thickening power, as a kind of novel foodstuff thickening agent, be used for food such as cold drinks and snachs, beverage, cake.
At present the part report is arranged, as being that to disclose a kind of Semen Trigonellae extract with effective biological active ingredients in 03144071.1 the patent application be the Semen Trigonellae total saponins at application number at the research to effective pharmacologically active principles of treatment diabetes in the Semen Trigonellae.This Semen Trigonellae extract can improve insulin sensitivity, blood sugar lowering and improve blood fat disorder.
In being 200410070104.6 patent application, application number disclosed a kind of Semen Trigonellae extract, its effective biological active ingredients is a polysaccharide 15～50%, total flavones 5～15% and saponin 10～30% think that 3 kinds of mutual synergism of composition in this extract have significant blood sugar lowering and effect for reducing fat.Its method of extracting Semen Trigonellae extract is that the Semen Trigonellae coarse powder is extracted with the alcohol-water solution percolation, and the percolate concentrating under reduced pressure is made with extra care and got through enrichment again.This extracting method has determined that the polysaccharide in the Semen Trigonellae extract is the polysaccharide compound of high polarity.
Diabetic nephropathy becomes serious threat patient life, has become the diabetics main causes of death.Though having used certain methods at present treats; but a lot of patients' nephropathy still carries out sexual development; China's angiotensin converting enzyme inhibitor commonly used and angiotensin-ii receptor blocker are protected the diabetes kidney at present; but it is only effective a part of patient; and it costs an arm and a leg, and side effect appears in the patient in part.Developing more effective medicine extremely needs.
Summary of the invention
The purpose of this invention is to provide a kind of have the Semen Trigonellae extract of medical value and from the Semen Trigonellae plant, extract method;
Another object of the present invention provides a kind of health food that is used for the treatment of diabetes, diabetic nephropathy drugs compositions and is used for blood sugar regulation, adjusting protection renal function and structure
Further purpose of the present invention provides the purposes of above-mentioned Semen Trigonellae extract aspect the health food of preparation treatment diabetes, diabetic nephropathy drugs and preparation blood sugar regulation, adjusting protection renal function and structure.
The inventor is by a large amount of pharmacodynamicss and pharmacological experiment research to Semen Trigonellae effective active composition, separation and Extraction goes out a kind of Semen Trigonellae extract with medical value from Semen Trigonellae, it is characterized in that containing in this extract the polysaccharide of 60%～80% percentage by weight.And this polysaccharide is the water-soluble following polysaccharide compound of middle polarity of energy in the Semen Trigonellae total polysaccharides.
The preparation method of Semen Trigonellae extract of the present invention may further comprise the steps:
A. Semen Trigonellae is decocted with conventional decocting method and obtain decocting liquid;
B. add ethanol in the decocting liquid behind filter cleaner and carry out precipitate and separate, concentration of alcohol reaches 10%～50% to its amount of alcohol added in the decocting liquid in order to make;
C. through the centrifugal supernatant that discards, its precipitation is washed after the centrifugal supernatant that discards is removed ethanol through distillation under vacuum and obtained precipitate with ethanol water;
D. above-mentioned precipitate after drying required Semen Trigonellae extract.
Wherein the amount of alcohol added described in the step b is preferably and makes the concentration of alcohol of decocting liquid reach 15%～30%.The biologic activity of the Semen Trigonellae polysaccharide that this concentration of alcohol is separated out is stronger.
Wherein drying mode described in the steps d is any conventional drying method, preferred drying under reduced pressure or spray drying.
The inventor finds that Semen Trigonellae extract of the present invention has the diabetes of control nephropathy, the function of protection diabetes kidney and the effect of structure.It is in particular in urinary protein excretion rate in the time of reducing the diabetes nephropathy; The serum creatinine and the urea nitrogen content that raise when reducing the diabetes nephropathy; Alleviate the loose and reduction glomerular volume of diabetes rat kidney; Alleviate the tissue pathologies change of diabetes rat kidney; Alleviate diabetes rat renal tissue ultrastructure (under the ultramicroscope) pathological changes and improve diabetes rat kidney anti-oxidative defense function, can raise diabetes rat renal cortex superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) and catalase (CAT) activity reduce renal cortex malonaldehyde (MDA) content.
The inventor finds that also Semen Trigonellae extract of the present invention has obvious reduction blood glucose in diabetic rats content; Can improve the effect of the glucose tolerance of diabetes rat.After medication 3 when week blood sugar content obviously reduce; And after 2 weeks of drug withdrawal, blood glucose gos up slower, and blood glucose continues in the time of reduced levels longer.
The present invention is used for the treatment of diabetes, diabetic nephropathy drugs compositions, wherein contains the above-mentioned Semen Trigonellae extract and the pharmacy acceptable auxiliary for the treatment of effective dose.
Semen Trigonellae extract of the present invention can be used for preparation treatment diabetes and diabetic nephropathy drugs.
The health food that Semen Trigonellae extract of the present invention can be used for preparing blood sugar regulation, regulates protection renal function and structure.
With described in the background technology two apply for a patent and compare, the Semen Trigonellae extract composition that the present invention obtains is simple, biological action is clear and definite.Its main component is 60% to 80% Semen Trigonellae polysaccharide, Semen Trigonellae polysaccharide biologically active.Except that having blood sugar lowering, improving diabetes rat blood fat disorder and the glucose tolerance, also have the effect of significant control diabetic nephropathy, diabetes and diabetic nephropathy thereof are had the better prevention effect.Because separation and Extraction of the present invention and preparation method are different from above-mentioned patent fully, the different of polyoses content and component in the Semen Trigonellae extract have been determined.Polysaccharide is mainly the following polysaccharide compound of middle polarity in the Semen Trigonellae extract that the present invention obtains.
The separation-extraction technology of the Semen Trigonellae extract that the present invention obtains is stable, easy, and cost is low; Dosage is little, and is nontoxic, has no side effect, and biological action is clear and definite., should have a good application prospect to the effect that prevents and treats diabetes and diabetic nephropathy and the exploitation of medicine and health food to further research Semen Trigonellae extract.
The specific embodiment
Can further understand essence of the present invention from following illustrative embodiment, but can not be considered as uniqueness of the present invention is limited.
Embodiment 1
The preparation of Semen Trigonellae extract of the present invention
Take by weighing X gram Semen Trigonellae, add about 5X gram water, heating makes boils, and keeps little 70min that boils, and decocting liquid is told, and the Semen Trigonellae part adds about 4X gram water again, and heating makes boils, and keeps little 50min that boils, and merges decocting liquid twice; The filtered water decocting liquid to wherein adding ethanol, makes determining alcohol reach 25%, and cooling is separated out precipitation; Centrifugal, discard supernatant.Its precipitation adds 25% washing with alcohol three times, and is centrifugal, discards supernatant.Precipitate is removed ethanol and drying under reduced pressure through distillation under vacuum, promptly obtains Semen Trigonellae extract.
The characterized of gained Semen Trigonellae extract is as follows:
1. the assay of Semen Trigonellae polysaccharose substance in the Semen Trigonellae extract
Adopt the sulphuric acid phynol method, concrete operations are as follows:
The preparation of standard solution: accurately take by weighing through 80 ℃ of exsiccant glucose 0.1000 grams, place the 100ml volumetric flask, thin up shakes up to scale; Promptly get 1000 μ g/ml glucose solutions.Be made into the standard series glucose solution through diluting repeatedly: 2.5 μ g/ml, 5 μ g/ml, 10 μ g/ml, 15 μ g/ml and 20 μ g/ml.
The preparation of testing sample: accurately get a certain amount of Semen Trigonellae extract respectively through suitable dilution, standardize solution shakes up.To be measured.
Measure: accurately measure standard series glucose solution and testing sample solution 0.50ml respectively, add 6% phenol solution 0.50ml, concentrated sulphuric acid 2.5ml, boiling water water-bath 15 minutes is put to room temperature, with the absorbance of spectrophotometric determination at the 490nm place.Make standard curve with the standard series glucose solution, calculate polyoses content.
The result: polyoses content is 60%～80% in the Semen Trigonellae extract;
2. Protein content is measured in the Semen Trigonellae extract
Adopt with Coomassie brilliant blue method (test kit), concrete operations are as follows:
The preparation of standard solution: measure accurately that concentration is 56mg/ml standard protein liquid 0.200ml in the test kit, add water and be settled to 10ml, promptly concentration is 1.12mg/ml.With 5 times of Coomassie brilliant blue reagent dilutions in the test kit, be made into Coomassie brilliant blue solution.
The preparation of testing sample: accurately get a certain amount of Semen Trigonellae extract respectively through suitable dilution, standardize solution shakes up.To be measured.
Measure: accurately measuring concentration respectively is 1.12mg/ml standard protein solution and testing sample solution 0.050ml, adds Coomassie brilliant blue solution 3ml, places 10 minutes, with the absorbance of spectrophotometric determination at the 595nm place.By formula calculate polyoses content:
Testing protein concentration (mg/ml)=testing protein absorbance ÷ standard protein absorbance * 1.12 results: protein content is 10%～15% in the Semen Trigonellae extract.
3. the further separation determination of Semen Trigonellae extract
(1) Deproteinization: remove protein in the Semen Trigonellae precipitate with conventional method of removing isolating protein.Supernatant separates through the pure sedimentation method, and reduction vaporization is sloughed pure and mild most of water, obtains deproteinize Semen Trigonellae precipitate.The method that routine is removed isolating protein comprises trichloroacetic acid method, Sevag method, trifluorotrichloroethane method, phenol and tannic acid method etc., preferred trichloroacetic acid method or Sevag method.
(3) the DEAE-32 cellulose separates: remove protein Semen Trigonellae precipitate solution and cross the DEAE-32 cellulose column, the NaCl solution with distilled water and variable concentrations carries out gradient elution respectively.The sulphuric acid phynol method detects.Obtain 5～10 polysaccharide components.
(4) determining molecular weight: with glucosan Dextran T-10, T-40, T-70, the T-90 standard substance are made into 1mg/ml solution respectively, cross Sephadex G-100 post, and 0.05mol/L NaCl eluting obtains the elution volume of 4 kinds of standard substance respectively.The molecular weight logarithm of standard substance is made the standard curve of molecular weight with the elution volume of standard substance.The above-mentioned polysaccharide component of sample is gone up Sephadex G-100 post respectively, and same eluent eluting obtains the elution volume of 5～10 kinds of samples, finds corresponding molecular weight according to the molecular weight standard curve.
The result: Semen Trigonellae extract contains the polysaccharide component that a plurality of molecular weight do not wait.Illustrate that main component is the Semen Trigonellae polysaccharide mixture in the Semen Trigonellae extract.
Embodiment 2
The proportioning and the method for making that contain the electuary of Semen Trigonellae extract
Get the aqueous solution that 2000ml contains 20% Semen Trigonellae extract, add the 16g stevioside, after treating to dissolve mixing fully, spray drying is dried to the extract powder with extracting solution, adds the 84g dextrin and makes the electuary granule, and it contains 66.7% Semen Trigonellae extract.Make 200 bags, every packed 1.5g.Can be used as medicine or health food is applied to the patient.Oral, 3 times on the one, each 1 bag, each one bag, add the water stirring and dissolving when taking.
Embodiment 3
The capsular proportioning and the method for making that contain Semen Trigonellae extract
The 100g Semen Trigonellae extract through being ground into fine powder, is granulated, encapsulated; The lactose or the dextrin that also can add 30～50g are granulated, and be encapsulated.Loading amount 0.35～0.40g; Can be used as medicine or health food is applied to the patient.Oral, 3 times on the one, each 3～4.
Embodiment 4
The proportioning and the method for making that contain the Semen Trigonellae extract tablet
Per ten thousand consumptions of every content of supplementary material
Semen Trigonellae extract 0.250g 2.5kg
Starch 0.050g 0.5kg
Starch slurry (17%) 0.080g 0.8kg
Magnesium stearate 0.050g 0.5kg
Microcrystalline Cellulose 0.030g 0.3kg
The Semen Trigonellae extract crushing fine powder is crossed 20 mesh sieves, and starch is crossed 120 mesh sieves, and mix homogeneously adds starch slurry, stir, make soft material, granulate by 16 mesh sieves, in 70 ℃ of dryings, dry granular adds magnesium stearate and microcrystalline Cellulose through 14 mesh sieve granulate, and mixing is with 12mm plane punch die tabletting.Can be used as medicine or health food is applied to the patient, oral, 3 times on the one, each 4.
Embodiment 5
Semen Trigonellae extract is to the activity research experiment of control diabetic nephropathy effect.
Select the Wistar healthy rat of body weight 170～200g, male and female half and half.Experimental group rat single intraperitoneal injection streptozotocin 50mg/kg (be dissolved in the 0.1mol/L citrate buffer solution, pH 4.2) prepares diabetes model.The injection streptozotocin detects blood glucose and glucose in urine after 3 days.Glucose in urine 〉=3+, blood glucose 〉=16.7mmol/L list the diabetes study object in.Matched group is the isopyknic above-mentioned buffer of lumbar injection simultaneously.
Experimental technique one: will become the diabetes rat of mould to be divided into the large, medium and small dosage treatment group of diabetic groups, positive control drug group and Semen Trigonellae extracting solution at random, other establishes the normal control group.The large, medium and small dosage treatment group of Semen Trigonellae extracting solution is irritated stomach and is given Semen Trigonellae extracting solution 1.5ml/100g body weight (being equivalent to Semen Trigonellae extract 394,197 and 98.5mg/kg body weight respectively).The positive control drug lotensin is irritated stomach and is given 15mg/kg body weight (1.5ml/100g body weight), and matched group and diabetic groups are only irritated stomach and given 1.5ml/100g body weight drinking water.Irritate stomach every day 1 time, totally 12 weeks.
Experimental technique two: will become the diabetes rat of mould to be divided into diabetic groups and Semen Trigonellae extracting solution treatment group at random, other establishes the normal control group.Semen Trigonellae extracting solution treatment group is irritated stomach and is given Semen Trigonellae extracting solution 1.5ml/100g body weight (being equivalent to Semen Trigonellae extract 394mg/kg body weight).Matched group and diabetic groups are only irritated stomach and are given 1.5ml/100g body weight drinking water.Experiment is totally 6 weeks.
Statistical procedures: data result is represented with x ± s, relatively adopts variance analysis between group.
1. Semen Trigonellae extract is to the influence of diabetes rat urinary protein excretion rate, serum creatinine and urea nitrogen content.
Press experimental technique one, collect twenty-four-hour urine liquid with metabolic cage, measure body weight, measure urine protein content, calculate twenty-four-hour urine PE rate (UAlb) with the Coomassie brilliant blue method the 12nd weekend.Pluck eyeball blood sampling back under the etherization and put to death animal, measure serum creatinine and blood urea nitrogen with Hitachi's 7150 type automatic biochemistry analyzers.The results are shown in Table 1.
The comparison of each treated animal urinary protein excretion rate of table 1, blood Scr and BUN (x ± s)
| Group | Number of animals | UAlb (mg/24h) | Serum creatinine (μ mol/L) | Blood urea nitrogen (mmol/L) |
| Dosage group small dose group in the heavy dose of group of matched group diabetic groups positive drug group | 8 8 10 10 10 10 | 7.3±3.2 17.2±3.3 ** 10.7±2.8 △△ 10.1±3.0 △△ 11.1±2.6 △△ 13.3±2.8 △△ | 25.9±2.8 51.9±5.7 ** 24.4±2.1 △△ 25.3±2.6 △△ 26.1±2.5 △△ 25.8±3.6 △△ | 6.6±1.4 17.8±3.0 ** 9.8±1.8 △△ 9.1±1.6 △△ 9.6±1.8 △△ 10.6±2.1 △△ |
Compare with matched group,
*P＜0.01; Compare with diabetic groups,
△P＜0.05,
△ △P＜0.01
Conclusion: the large, medium and small dosage treatment group of Semen Trigonellae extracting solution urinary protein excretion rate, serum creatinine and urea nitrogen content all obviously reduce, and the effect of its effect and positive control drug lotensin is close.
2. Semen Trigonellae extract is to the influence of the kidney and the glomerule hypertrophy of diabetes rat.
Press experimental technique one, get kidney and claim kidney heavy, calculate kidney weight/body weight ratio.Conventional preparation paraffin section, HE, PAS dyeing, om observation.Measure glomerule sectional area [A] with a counting method, each kidney is measured 30 glomerule at least, presses V=β/k * [A]
3/2Calculate glomerule average external volume V.The results are shown in Table 2.
The comparison of each treated animal kidney weight/body weight of table 2 and glomerular volume (x ± s)
| Group | Number of animals | Kidney weight/body weight (mg/g) | Glomerular volume (μ m 3×10 3) |
| Dosage group small dose group in the heavy dose of group of matched group diabetic groups positive drug group | 8 8 10 10 10 10 | 2.72±0.61 5.94±0.51 ** 4.78±0.44 △△ 4.69±0.41 △△ 5.24±0.62 △△ 5.63±0.84 | 480.6±99.6 716.2±112.5 ** 522.4±106.1 △△ 516.3±106.2 △△ 574.6±100.8 △△ 696.8±115.6 |
Compare with matched group,
*P＜0.01; Compare with diabetic groups,
△P＜0.05,
△ △P＜0.01
Conclusion: the big or middle dosage treatment group of Semen Trigonellae extracting solution kidney weight/body weight ratio and glomerular volume all obviously reduce, its effect near or be better than the effect of positive control drug lotensin, show that the Semen Trigonellae extracting solution can alleviate the kidney of diabetes rat and the hypertrophy of glomerule.
3. Semen Trigonellae extract is to the influence of diabetes rat kidney anti-oxidation function.
Press experimental technique one, get kidney and separate renal cortex, preparation renal cortex homogenate.Carrying out following index detects: measure renal cortex superoxide dismutase (SOD) activity with pyrogallol from the oxygen quenching method 1..2. measure glutathion peroxidase (GSH-Px) activity with the DTNB direct method.3. detect catalase (CAT) activity with tetraborate substrate method.4. measure malonaldehyde (MDA) content with the TBA method.The results are shown in Table 3.
Each treated animal renal cortex MDA content of table 3, CAT, GSH-Px and the active comparison of SOD (x ± s)
| Group | Number of animals | MDA (nmol·g -1tis) | CAT (U·mg -1pro) | GSH-Px (U·mg -1pro) | SOD (U·mg -1pro) |
| Dosage group low dose group in the matched group diabetic groups positive control high dose group | 8 8 10 10 10 10 | 0.63±0.01 0.89±0.18 ** 0.65±0.10 △△ 0.63±0.09 △ 0.68±0.06 △ 0.65±0.15 △ | 5.83±0.70 2.52±0.86 ** 3.88±0.07 △△ 4.34±0.69 △ 3.19±0.27 3.46±0.91 | 0.087±0.024 0.054±0.010 ** 0.073±0.009 △△ 0.071±0.006 △ 0.053±0.010 0.053±0.013 | 25.40±2.13 18.29±3.63 ** 23.04±6.23 24.99±2.34 △ 20.21±2.35 21.43±5.37 |
Matched group compares, with
*P＜0.01; Compare with diabetic groups,
△P＜0.05,
△ △P＜0.01
Conclusion: Semen Trigonellae extracting solution can raise diabetes rat renal cortex superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) and catalase (CAT) activity, reduce renal cortex malonaldehyde (MDA) content.Show that the Semen Trigonellae extracting solution can improve diabetes rat kidney activities of antioxidant enzymes, suppress lipid peroxidation, the diabetes rat nephropathy is had the improvement effect.
4. Semen Trigonellae extract is to the influence of renal tissue pathological change.
Press experimental technique one, diabetes rats glomerular mesangium district PAS stained positive material showed increased under the optical microscope, mesangial region is obviously widened, and the part blood capillary subsides, the swelling of part renal cells, the visible albumen sample of minority renal tubules intracavity cast.The rat of positive control drug lotensin group, the large, medium and small dosage treatment group of Semen Trigonellae extracting solution, nephropathy all has clear improvement, and glomerular mesangium district PAS stained positive material obviously reduces, and mesangial region does not have obvious broadening, blood capillary is open, and the renal cells pathological changes obviously alleviates.
Conclusion: Semen Trigonellae extract can alleviate the loose and reduction glomerular volume of diabetes rat kidney; Alleviate the tissue pathologies change of diabetes rat kidney.
5. Semen Trigonellae extract is to the influence of glomerule ultrastructure pathological changes.
Press experimental technique one, conventional preparation kidney transmission electron microscope specimen is with Japanese JEM-1200EX type transmission electron microscope observation glomerule pathological changes.Under the transmission electron microscope, the diabetes rats glomerular basement membrane is segmental and thickens, mesentery substrate showed increased, and the epithelium podocytic process is fusion in various degree.The effect that all has clear improvement of the glomerule ultrastructure of positive control drug group, the large, medium and small dosage treatment group of Semen Trigonellae extracting solution, the effect of wherein big or middle dosage group is the most obvious.
Conclusion: Semen Trigonellae extract can alleviate diabetes rat renal tissue ultrastructure pathological changes.
Embodiment 6
The hypoglycemic activity research experiment of Semen Trigonellae extract.
1. Semen Trigonellae extract is to the time-effect relationship of blood glucose in diabetic rats influence.
Press embodiment 5 experimental techniques one, before administration and the 3rd week, 4 weeks, 6 weeks after the administration, 8 weeks and 12 weeks respectively detected blood sugar content, the results are shown in Table 4.
The time-effect relationship 1 that table 4 Semen Trigonellae extract influences blood glucose in diabetic rats (x ± s)
| Group | Number of animals | Before the administration | Blood sugar concentration (mmol/L) | ||||
| 3 weeks | 4 weeks | 6 weeks | 8 weeks | 12 weeks | |||
| Matched group diabetic groups extract group | 8 8 10 | 6.90±0.61 20.75±2.79 △△20.66±3.02 △△ | 5.73±0.40 18.58±3.44 △△ 12.14±3.33 **△△ | 7.23±1.10 19.53±3.81 △△7.68±2.02 ** | 6.18±0.57 17.40±2.12 △△ 8.58±2.37 ** | 6.42±1.28 18.04±2.63 △△ 7.39±3.27 ** | 6.12±0.15 18.09±4.10 △△ 7.42±2.68 ** |
Compare with diabetic groups,
*P＜0.05,
*P＜0.01; Compare with matched group,
△P＜0.05,
△ △P＜0.01
Conclusion: Semen Trigonellae extract can significantly reduce blood glucose in diabetic rats content.After medication 3 when week blood sugar content significantly reduce, reducing to matched group during 4 weeks does not have marked difference, and lasts till for 12 weeks.
Press embodiment 5 experimental techniques two, preceding 4 all administrations are irritated stomach every day 1 time, back 2 all drug withdrawals, totally 6 weeks.In the 1st week, 3 weeks, 4 weeks, 5 weeks with respectively detect blood glucose and glucose in urine content 6 weeks, the results are shown in Table 5.
The time-effect relationship 2 that table 5 Semen Trigonellae extract influences blood glucose in diabetic rats (x ± s)
| Group | Number of animals | Before the administration | Blood sugar concentration (mmol/L) | ||||
| 1 week | 3 weeks | 4 weeks | 5 weeks | 6 weeks | |||
| The heavy dose of group of matched group diabetic groups | 8 8 10 | 5.76±1.63 19.73±2.90 △△19.88±1.49 △△ | 5.76±1.63 16.81±3.21 △△17.33±1.02 △△ | 4.6±0.99 18.45±3.11 △△ 11.68±3.50 **△△ | 4.45±1.77 15.8±2.29 △△ 7.67±1.93 ** | 6.9±0.14 18.3±2.14 △△ 9.82±0.72 ** | 6.45±1.34 18.39±2.73 △△ 10.97±1.68 ** |
Compare with diabetic groups,
*P＜0.05,
*P＜0.01; Compare with matched group,
△P＜0.05,
△ △P＜0.01
Conclusion: Semen Trigonellae extract can significantly reduce blood glucose in diabetic rats content.After medication 3 when week blood sugar content significantly reduce; And 2 weeks of drug withdrawal, it is slower that blood glucose gos up, and it is longer that blood glucose continues significantly to be lower than time of diabetes rat.
2. Semen Trigonellae extract is to the diabetes rat glucose tolerance test
Press embodiment 5 experimental techniques two, do glucose tolerance test 4 weekends, rat gives behind the glucose to measure blood sugar content in 30 minutes, 60 minutes, 90 minutes and 120 minutes, the results are shown in Table 6.
The administration of table 6 Semen Trigonellae extract is to the result of diabetes rat glucose tolerance test (x ± s)
| Group | Number of animals | Before giving sugar | Blood sugar concentration (mmol/L) | |||
| 30min | 60min | 90min | 120min | |||
| The heavy dose of group of matched group diabetic groups | 8 8 10 | 6.15±0.79 17.66±2.77 △△7.66±1.48 ** | 11.58±1.89 24.23±2.71 △△ 19.63±1.06 ** △△ | 10.62±1.51 23.17±2.34 △△ 18.62±1.31 ** △△ | 7.85±0.77 20.11±3.61 △△ 13.16±1.39 ** | 7.65±0.77 22.20±1.75 △△ 9.70±1.33 ** |
Compare with diabetic groups,
*P＜0.05,
*P＜0.01; Compare with matched group,
△P＜0.05,
△ △P＜0.01
Conclusion: Semen Trigonellae extract can improve the glucose tolerance of diabetes rat.
Prove that through above-mentioned a large amount of pharmacodynamics and pharmacological experiment the biologic activity of Semen Trigonellae extract is as follows:
1. can reduce diabetes rat urinary protein excretion rate.
2. serum creatinine that raises in the time of reducing the diabetes nephropathy and urea nitrogen content.
3. can alleviate the loose and reduction glomerular volume of diabetes rat kidney.
4. can alleviate the tissue pathologies change of diabetes rat kidney.
5. can alleviate diabetes rat renal tissue ultrastructure (under the ultramicroscope) pathological changes.
6. can treat the diabetes nephropathy, the function and the structure of protection diabetes kidney.
7. extract A can improve diabetes rat kidney anti-oxidative defense function, can raise diabetes rat renal cortex superoxide dismutase (SOD), glutathion peroxidase (GSH-Px) and catalase (CAT) activity reduce renal cortex malonaldehyde (MDA) content.
8. can obviously reduce blood glucose in diabetic rats content; Can improve the glucose tolerance of diabetes rat.After medication 3 when week blood sugar content obviously reduce; And after 2 weeks of drug withdrawal, blood glucose gos up slower, and blood glucose continues in the time of reduced levels longer.
In sum, Semen Trigonellae extract can obviously reduce diabetes rat blood sugar level, improve diabetes rat blood fat disorder and protection renal function and structure, show combination therapy effect to main biochemical, the pathological change of diabetes.Above-mentioned result of study shows that Semen Trigonellae is to diabetes and chronic complicating diseases thereof---diabetic nephropathy has the better prevention effect.
Claims (8)
1. Semen Trigonellae extract that extracts from Semen Trigonellae is characterized in that containing in this extract the polysaccharide of 60%～80% percentage by weight.
2. Semen Trigonellae extract according to claim 1 is characterized in that said polysaccharide is the water-soluble following polysaccharide compound of middle polarity of energy in the Semen Trigonellae total polysaccharides.
3. the preparation method of the described Semen Trigonellae extract of claim 1 is characterized in that may further comprise the steps:
A. Semen Trigonellae is decocted with conventional decocting method and obtain decocting liquid;
B. add ethanol in the decocting liquid behind filter cleaner and carry out precipitate and separate, concentration of alcohol reaches 10%～50% to its amount of alcohol added in the decocting liquid in order to make;
C. through the centrifugal supernatant that discards, its precipitation is washed after the centrifugal supernatant that discards is removed ethanol through distillation under vacuum and obtained precipitate with ethanol water;
D. above-mentioned precipitate after drying required Semen Trigonellae extract.
4. preparation method according to claim 3 is characterized in that wherein the amount of alcohol added described in the step b is preferably to make the concentration of alcohol of decocting liquid reach 15%～30%.
5. be used for the treatment of diabetes, diabetic nephropathy drugs compositions, wherein contain described Semen Trigonellae extract of the claim 1 for the treatment of effective dose and pharmacy acceptable auxiliary.
6. the application of the described Semen Trigonellae extract of claim 1 in preparation treatment diabetes and medicine for treating diabetic nephropathy.
7. be used for blood sugar regulation, regulate the health food of protection renal function and structure, wherein contain the described Semen Trigonellae extract of claim 1 and the health food acceptable additive of effective dose.
8. the described Semen Trigonellae extract of claim 1 has blood sugar regulation in preparation, regulates the application in the health food of protecting renal function and structure function.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100170766A CN100360145C (en) | 2005-08-25 | 2005-08-25 | Fenugreek seed extract and its preparing process and application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2005100170766A CN100360145C (en) | 2005-08-25 | 2005-08-25 | Fenugreek seed extract and its preparing process and application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1733055A true CN1733055A (en) | 2006-02-15 |
| CN100360145C CN100360145C (en) | 2008-01-09 |
Family
ID=36075810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2005100170766A Expired - Fee Related CN100360145C (en) | 2005-08-25 | 2005-08-25 | Fenugreek seed extract and its preparing process and application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100360145C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008015699A3 (en) * | 2006-06-13 | 2008-09-12 | Babulal Bhawarlal Jain | A process for manufacturing an herbal composition for curing diabetes and herbal composition made thereof |
| CN101703208B (en) * | 2009-11-20 | 2012-02-08 | 天津农学院 | Method for preparing edible tonka bean powder |
| CN102816253A (en) * | 2012-09-11 | 2012-12-12 | 西北师范大学 | Method for extracting bottle gourd Pakistan rubber by dual-enzyme coupling |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1456570A (en) * | 2003-02-18 | 2003-11-19 | 何昌义 | Extraction of saponin from seeds of trigonella foenum-graecum |
| CN1227260C (en) * | 2003-08-01 | 2005-11-16 | 新疆特丰药业股份有限公司 | Etractive of fenugreek and its producing method |
| CN1290525C (en) * | 2004-07-13 | 2006-12-20 | 赵余庆 | A blood lowering botanical extract and its preparation and preparing process |
| CN100434091C (en) * | 2004-07-30 | 2008-11-19 | 北京星昊医药股份有限公司 | Fenugreek seed extract and its preparing process and application |
| CN100467046C (en) * | 2005-06-09 | 2009-03-11 | 陕西爱波卓科技有限责任公司 | Sugar decreasing composition of fenugreek seed |
-
2005
- 2005-08-25 CN CNB2005100170766A patent/CN100360145C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008015699A3 (en) * | 2006-06-13 | 2008-09-12 | Babulal Bhawarlal Jain | A process for manufacturing an herbal composition for curing diabetes and herbal composition made thereof |
| CN101703208B (en) * | 2009-11-20 | 2012-02-08 | 天津农学院 | Method for preparing edible tonka bean powder |
| CN102816253A (en) * | 2012-09-11 | 2012-12-12 | 西北师范大学 | Method for extracting bottle gourd Pakistan rubber by dual-enzyme coupling |
| CN102816253B (en) * | 2012-09-11 | 2014-04-16 | 西北师范大学 | Method for extracting fenugreek gum by dual-enzyme coupling |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100360145C (en) | 2008-01-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104664512B (en) | A kind of Radix Glycyrrhizae plant beverage improving immunity and preparation method thereof | |
| CN1310668C (en) | Medicine for treating diabetes, and its prepn. method | |
| CN1559539A (en) | Medicinal mixture possessing alpha glycocidase inhibiting activity and its use | |
| CN1394623A (en) | Health-care food capable of regulating blood sugar and preparing method thereof | |
| CN113616688B (en) | Anserine composition for repairing kidney injury and application thereof | |
| CN110214845A (en) | A method of having effects that the health preserving tea preparation for treating gout | |
| CN104082820A (en) | Hypoglycemic soluble granules containing alfalfa | |
| CN1733055A (en) | Fenugreek seed extract and its preparing process and application | |
| CN102813112B (en) | Foodstuff for diabetic nephropathy | |
| CN111450136A (en) | Oyster and kudzu root composite particles and preparation method thereof | |
| CN1186091C (en) | Chinese medicine injection and its production process | |
| CN113559152B (en) | Liver-protecting traditional Chinese medicine composition and beverage as well as preparation method and application thereof | |
| CN110251530A (en) | A kind of preparation method and its clinical application of larch arabinogalactan composition | |
| CN101559166B (en) | Ophiopogon japonicus extract, preparation method and hypoglycemic application thereof | |
| CN107875144A (en) | A kind of combination of oral medication for treating depression | |
| CN1087627C (en) | Medicament for treating diabetes and its preparation method | |
| CN106309546A (en) | Extract for treating diabetic nephropathy | |
| CN1298351C (en) | Chinese medicine oral preparaton for treating urinary system infestation and its preparation method | |
| CN1899385A (en) | Process for preparing Chinese medicine compound injection for treating chronic renal failure and use | |
| CN1557378A (en) | Medicine for treating diabetes | |
| CN1840008A (en) | Chinese medicinal granule for clearing pharynx and moistening throat and preparation method thereof | |
| CN1557824A (en) | Silkworm excrement total alkaloid and its preparation method | |
| CN1265805C (en) | Chinese medicine oral preparation for lonering blood sugar and its preparation method | |
| CN101219157B (en) | method for preparing product for reducing blood sugar | |
| Ahmad et al. | Effect of Artemisia herb on induced hyperglycemia in wistar rats |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080109 Termination date: 20130825 |