CN104664512B - A kind of Radix Glycyrrhizae plant beverage improving immunity and preparation method thereof - Google Patents
A kind of Radix Glycyrrhizae plant beverage improving immunity and preparation method thereof Download PDFInfo
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- CN104664512B CN104664512B CN201510105185.7A CN201510105185A CN104664512B CN 104664512 B CN104664512 B CN 104664512B CN 201510105185 A CN201510105185 A CN 201510105185A CN 104664512 B CN104664512 B CN 104664512B
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Abstract
The invention discloses a kind of Radix Glycyrrhizae plant beverage improving immunity and preparation method thereof.With Radix Glycyrrhizae for primary raw material, the composite Radix Glycyrrhizae concentrated juice of science, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer etc., adopt modern low temperature and biological extraction technology to Feedstock treating, drastically increase the content of material effective component, organic mixing of various functional components, play the synergism of the best, have employed the heating power mixing concentration of unstable concentrated solution simultaneously, cold, the combined removal that heat is muddy, the methods such as aseptic filtration, significantly enhance the local flavor of Radix Glycyrrhizae plant beverage, extend the shelf-life of product, it is prepared for a kind of Radix Glycyrrhizae plant beverage improving the effects such as human body overall immunity.
Description
Technical field
The present invention relates to Radix Glycyrrhizae plant beverage, be specifically related to a kind of Radix Glycyrrhizae plant beverage improving immunity and preparation method thereof.
Background technology
The famous medical science ancient books and records " Huangdi Neijing Taisu, Grand Simplicity of Inner Canon of Huangdi " of Ancient Times in China were once mentioned, " food is food on an empty stomach, and patient's food is medicine ".Ministry of Public Health once to integration of edible and medicinal herbs article, the article that can be used for health food and health food disable article made concrete regulation, be wherein food but medicine just up to nearly hundred kinds.Since integration of edible and medicinal herbs, then how to utilize integration of edible and medicinal herbs article and can be used for the article of health food in order to prevent and cure diseases, building body, just become a big problem of people's health preserving.
The shown Compendium of Material Medica of Ming Dynasty's Li Shizhen (1518-1593 A.D.) is so introduced Radix Glycyrrhizae " abnormal smells from the patient is sweet, flat, nontoxic ".Radix Glycyrrhizae has the good reputation of the king of Chinese herbal medicine, tcm prescription has " ten sides nine grass ", " without grass not Cheng Fang " say that 110 prescriptions in treatise on Febrile Diseases just have 74 prescriptions to use Radix Glycyrrhizae.Tang of Sui well-known doctor Zhen Quan says: " in all medicines, Radix Glycyrrhizae is monarch, controls 72 kinds of mammary calculus poison, solves 1,002 vegetation poison by every means, and many medicines that is in harmonious proportion are gained merit, thus have state old number.”
Jin Dynasty physician Li Gao says: Radix Glycyrrhizae life pathogenic fire purging heat；Ripe use dissipates exterior cold, goes pharyngalgia, evil removing heat, slow healthy energy, yin nourishing blood, tonifying the spleen and stomach, lung moistening.Shennong's Herbal claims Radix Glycyrrhizae " clothes are made light of one's life by commiting suicide and prolonged life for a long time ".One Chinese medicine study person represents, Radix Glycyrrhizae ranks the top grade of Chinese herbal medicine, and cheap, and Radix Glycyrrhizae is successfully introduced into beverage, is to Chinese medicine health preserving one contribute greatly.Radix Glycyrrhizae plant beverage can throat-clearing lung-moistening, QI invigorating is gone dry.Radix Glycyrrhizae can rise and can drop, and does not only have the effect that antiinflammatory is reduced internal heat, also has greatly effect of invigorating the spleen and replenishing QI.Nowadays the air-polluting such as vehicle exhaust is continuously increased, and the increase of stress, causes that the people suffering from pharyngitis for a long time is many especially, only alleviates pharyngitis misery exactly, and Radix Glycyrrhizae plant beverage just has enough market spaces.
At present, more with the plant beverage that Radix Glycyrrhizae is prepared for primary raw material, application number be 200710199401.4 Chinese invention patent disclose a kind of natural plant beverage, the configuration proportion of its compositions and unit of weight thereof is: Semen Raphani 3 parts, Fructus Crataegi 10 parts, 5 parts of Fructus Hordei Germinatus, Fructus Setariae Germinatus 5 parts, 10 parts of Radix Glycyrrhizae, Rhizoma Dioscoreae 10 parts.Manufacture method: said components is cleaned mixing, insert in rustless steel container, the drinking water adding its weight ratio 35 times soaks 30 minutes, then again with slow fire heating to 100 degree of high temperature, after decocting 30 minutes, re-use filter to be leached by aqueous solution, use vacuum to seal filling apparatus by solution fill to bottle.Application number be 201310245557.7 Chinese invention patent disclose a kind of Radix Glycyrrhizae composite plant beverage formula and preparation technology, described Radix Glycyrrhizae composite plant beverage is by raw material Radix Glycyrrhizae, Radix Et Rhizoma Fagopyri Tatarici, Fructus Lycii and Flos Lonicerae, after lixiviate, add proper honey, white sugar adjusts mouthfeel, makes Radix Glycyrrhizae composite plant beverage through operations such as sterilization, canned, sterilizings.Plant beverage prepared by the present invention is without synthetic preservative and antibacterial, and safe for drinking is useful, has blood sugar lowering, blood fat reducing, heat clearing away, the health-care effect relieving summer heat, detoxify.Application number be 201310588217.4 Chinese invention patent disclose a kind of Balloonflower root-licorice root plant beverage, the raw material of preparing of this Balloonflower root-licorice root plant beverage is made up of Radix Platycodonis, Radix Glycyrrhizae, and it is prepared by method and obtains: 1. clean；2. clean；3. infiltration: being respectively placed in demulcen groove by Radix Platycodonis, Radix Glycyrrhizae according to predetermined weight number, infiltrate 1h respectively, wherein predetermined weight number is Radix Platycodonis is 4～15 parts, 4～15 parts of Radix Glycyrrhizae；4. cutting: the Radix Platycodonis after infiltration is made sheet, and Radix Glycyrrhizae is cut into segment, holds respectively；5. extracting: be positioned in extraction pot by the Radix Platycodonis after cutting, Radix Glycyrrhizae, add the soak by water 2 times of Radix Platycodonis, Radix Glycyrrhizae summation weight 10 times, 2 hours first times, second time 1 hour, after twice filtrate merges, cold preservation stands 12h, Aspirate supernatant, is easily separated, obtains extracting solution；6. dispensing: added by extracting solution in material-compound tank, after adding 3%～10% white sugar, 0.4 ‰～2.5 ‰ edible essences, purified water mixing, stirs to obtain Balloonflower root-licorice root plant beverage.
Above-mentioned disclosed Radix Glycyrrhizae plant beverage is functional single, adopts traditional water to carry, leach extraction method processes raw material, and material effective component utilization rate is low, and the wasting of resources is big.
Summary of the invention
Solved by the invention technical problem is that overcomes functional single, the defect that raw material availability is low of existing Radix Glycyrrhizae plant beverage, with Radix Glycyrrhizae for primary raw material, the composite Radix Glycyrrhizae concentrated juice of science, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer etc., adopt modern low temperature and biological extraction technology to Feedstock treating, drastically increase the content of material effective component, prepare a kind of Radix Glycyrrhizae plant beverage improving immunity.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of Radix Glycyrrhizae plant beverage improving immunity, is mainly prepared by the raw material of following parts by weight:
Radix Glycyrrhizae concentrated juice 3-20 part, fungi extracts 10-30 part, concentrated medlar juice 5-15 part, modified dietary fiber 5-15 part, bee pollen of maize extract 10-30 part, pectin decomposer 2-8 part；
Preferably, the described Radix Glycyrrhizae plant beverage improving immunity, mainly prepared by the raw material of following parts by weight:
Radix Glycyrrhizae concentrated juice 8-16 part, fungi extracts 15-25 part, concentrated medlar juice 8-12 part, modified dietary fiber 8-12 part, bee pollen of maize extract 15-25 part, pectin decomposer 4-6 part；
It is highly preferred that the described Radix Glycyrrhizae plant beverage improving immunity, mainly prepared by the raw material of following parts by weight:
Radix Glycyrrhizae concentrated juice 12 parts, fungi extracts 20 parts, concentrated medlar juice 10 parts, modified dietary fiber 10 parts, bee pollen of maize extract 20 parts, pectin decomposer 5 parts；
Further, the described Radix Glycyrrhizae plant beverage improving immunity also includes the adjuvant of following parts by weight: white sugar 50-250 part, Fructus Momordicae 8-18 part, Mel 3-15 part, citric acid 1-3 part；
Preferably, the described Radix Glycyrrhizae plant beverage improving immunity also includes the adjuvant of following parts by weight: white sugar 100-200 part, Fructus Momordicae 12-15 part, Mel 8-10 part, citric acid 1.5-2.5 part；
It is highly preferred that the described Radix Glycyrrhizae plant beverage improving immunity also includes the adjuvant of following parts by weight: white sugar 150 parts, Fructus Momordicae 13 parts, Mel 9 parts, citric acid 2 parts；
Further, described Radix Glycyrrhizae concentrated juice is to prepare through the extract at low temperature technology such as ultrasonic cleaning, freezing, pulverizing, high-pressure pulse electric extraction, ultrasonic wave added microwave extraction, low temperature enzymolysis with Radix Glycyrrhizae for primary raw material, can retain glycyrrhizic acid, enoxolone, liquirtin, isoliquiritin, Angelica Polysaccharide and other antioxidation, volatile, thermal sensitivity effective ingredient to greatest extent；
Preferably, the preparation method of described Radix Glycyrrhizae concentrated juice, comprise the steps: Radix Glycyrrhizae tabellae, Flos Lonicerae and Flos Caryophylli to be respectively put in the ultrasonic washing unit equipped with 0.3-0.5% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz, drain, 6-10:1-3:1-2 Homogeneous phase mixing in mass ratio, then in-18-23 DEG C of freezing 5-10min；It is crushed to particle diameter 0.3-1mm immediately；Put in container and add the water of 3-5 times of weight, obtaining mixed material, being 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, carry out high voltage pulse electric field processing when pulse frequency 200-300Hz；Then heat to 30-40 DEG C, insulation, microwave extraction is carried out when power 150-300W, wherein, each microwave exposure total time 60-80s, compartment irradiation is carried out: irradiation 10s, interval 10s, such irradiation 10 times, simultaneously at power 200-300W, carries out ultrasonic assistant extraction when frequency 30-40KHz；It is continuously heating to 40-50 DEG C, insulation, it is subsequently adding the compound enzyme enzymolysis 30-50min of mixed material gross weight 2-5%, filters, obtain filtrate；Filtering residue rinses 3 times with 1-3 times of weight 76-80 DEG C of water, and rinsing liquid merges with filtrate, Homogeneous phase mixing, and being evaporated to solid content is more than 20%, obtains Radix Glycyrrhizae concentrated juice；
Described compound enzyme is by pectase, cellulase, tannase, amylase and protease 3-7:3-5:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio.
Further, described fungi extracts is with repair cell tissue, has antiulcer action, antioxidation, effect of scavenging radical and anti-aging effects, can be obviously improved that human body entirety epidemic disease exempts from power and physical strength reinforcing, the edible fungi that improves gastrointestinal function is prepared through ultrasonic cleaning, high voltage pulse electric field processing and biological enzymolysis；
Preferably, the preparation method of described fungi extracts comprises the steps: Hericium erinaceus (Bull. Ex Fr.) Pers., Semen Ginkgo and Auricularia to be respectively put in the ultrasonic washing unit equipped with 0.3-0.5% sodium bicarbonate solution and cleans 5-10min in 200W, 40KHz, drain, being cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 11-13:4-6:2-3 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min, pulverizing immediately, ground product particle diameter is 0.2-1mm；The water adding ground product quality 3-5 times obtains mixture, adjusting mixture pH is 4.5-6, at electric field intensity 35-45kV/cm under room temperature, burst length 500-700 μ s, carry out high voltage pulse electric field processing when pulse frequency 200-400Hz, it is warming up to 45～55 DEG C, the mixed enzyme enzymolysis of addition mixture weight 0.5-1.2% 4～6 hours, obtains edible fungi enzymolysis solution, filters, filtrate is more than 30% through being evaporated to solid content, obtains fungi extracts；
In described edible fungi enzymolysis solution, edible fungi polysaccharide content is up to 7.13%；
Described mixed enzyme parts by weight consist of: papain 20-30 part, bromelain 20-30 part, glucanase 10-15 part, xylanase 10-15 part, pentosanase 10-15 part, ficin 5-10 part, midrange thermal stable amylase 5-10 part, pectase 5-10 part, tannase 3-5 part.
Further, the preparation method of described concentrated medlar juice, comprise the steps: Fructus Lycii, the Radix Astragali, Ganoderma sporophore 10-12:1-3:1-2 Homogeneous phase mixing in mass ratio, after flushing, quick freeze is to-40--30 DEG C；Sublimation drying, vacuum 400-500Pa, it is dried to moisture < 12%；It is crushed to particle diameter in-10--2 DEG C and obtains ground product at below 1.5mm；Add the aqueous citric acid solution of ground product weight 3-6 times, the pH value controlling solution is 4-5, add the compound enzyme of mixture weight 0.5-1%, described compound enzyme is made up of acid pectase, neutral protease and cellulase, the weight ratio of three kinds of enzymes is 2.5: 1: 1.5, hydrolysis temperature is 40-48 DEG C, and enzymolysis time is 1.5-2hr；Afterwards pH value being adjusted to 5.1-5.6, temperature is raised to 50-60 DEG C, adds the glucanase of the pectase of mixture weight 0.2-0.5%, 0.2-1% simultaneously, and action time is 2.5-3hr；Enzymolysis solution is 5-10 DEG C of filtration；Filtrate freeze concentration obtains compound concentrated medlar juice.
Further, described modified dietary fiber is that dietary fiber is processed through physics, chemical or biological method and obtained, soluble fiber cellulose content is high, biological activity is strong, beneficial bacteria of intestinal tract group has cellulose important, positive role, compared with full diet fiber, its biological action is more powerful；
Preferably, described modified fibre is to be obtained through enzyme enzymolysis by one or more in inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber；
More preferably, the preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber 8-10:4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, add its water of quality 3-7 times, room temperature 200-300W, 35-40KHz condition supersound extraction 10-15min, then at electric field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction when pulse frequency 200-300Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution concentrating under reduced pressure, lyophilization, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber；
Described enzyme is xylanase, cellulase, laccase, pectase, tannase 6-10:3-5:2-4:2-4:1-3 Homogeneous phase mixing in mass ratio.
Further, described bee pollen of maize extract be with bee pollen of maize, Glutinous Semen Maydis must, defatted corn powder mixture for raw material, successively through ultrasonic cleaning, high-pressure pulse electric and supersound process, biological enzymolysis and prepared；
nullPreferably，The preparation method of described bee pollen of maize extract comprises the steps: that 40-60:3-5:2-4 weighs bee pollen of maize respectively in mass ratio、Glutinous Semen Maydis must with defatted corn powder，First by bee pollen of maize、Glutinous Semen Maydis must be put in the ultrasonic washing unit equipped with 0.3-0.5% sodium bicarbonate solution respectively，In 200W under room temperature、40KHz cleans 3-5min，Drain，Then mix with defatted corn powder，Add the water of former bee pollen of maize quality 3-5 times，It is 4-6 with breast acid for adjusting pH value，In electric field intensity 20-40kV/cm，Burst length 400-500 μ s,High voltage pulse electric field processing is carried out when pulse frequency 200-300Hz，Then room temperature 400W、40KHz condition supersound process 10-15min；Being warming up to 40-50 DEG C, add the enzyme preparation of former bee pollen of maize quality 2-8%, stir, enzymolysis 15-20min, enzymolysis solution liquid filters through 100-300 eye mesh screen, and namely filtrate obtain bee pollen of maize extract through ultrafiltration, concentrating under reduced pressure, lyophilization；
The quality group of described enzyme preparation becomes: protease: saccharifying enzyme: pectase: cellulase: lipase=11-13:5-10:4-6:3-5:1-3.
Further, described pectin decomposer is to obtain pectin with waste residues such as the peels such as Fructus Citri Limoniae, orange, Fructus Citri grandis, mandarin orange, Fructus Vitis viniferae or Fructus Crataegi, Radix Betae, Fructus Mali pumilaes through ultrasonic cleaning, high-voltage pulse extraction, hot water extraction, precipitate with ethanol, then prepared through pectinase enzymatic hydrolysis；
Preferably, the preparation method of described pectin decomposer, comprise the steps: fresh Fructus Crataegi marc, Pericarpium Vitis viniferae, pomace, megasse to be respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz, drain, being cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 6-9:3-5:2-4:2-4 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, particle diameter is 0.1-1mm；nullAdd the deionized water of ground product quality 3-5 times，Mix homogeneously，It is 2-3 with Fructus Citri Limoniae acid for adjusting pH value，At electric field intensity 35-45kV/cm under room temperature，Burst length 400-600 μ s,High voltage pulse electric field processing is carried out when pulse frequency 200-300Hz，Then normal pressure boils 30-50min，Bag filter，Filtering residue boils 40-60min with its filtrate normal pressure of quality 1-3 times,Bag filter，Merging filtrate，Kieselguhr filters，Filtrate is made to clarify，Being evaporated to solid content at 75 DEG C is 6-10%，It is rapidly cooled to room temperature，Add 95% ethanol that pH value is 2-3 of 1.5 times of volumes of concentrated solution，Stand 20-30min,Pectin is made to be precipitated out，4000r/min is centrifuged 5-10min，Reclaim precipitation，With the dehydrated alcohol eluting of 2 times of volumes，Centrifugal，Reclaim precipitation，So operation 2 times，What obtain is deposited in 40-60 DEG C of vacuum drying 2-3h,Obtain pectin，Add 40-50 DEG C of water of pectin quality 4-5 times，Stirring，Homogeneous phase mixing，Insulation，It is 4.5-5.5 with breast acid for adjusting pH value，Add the pectinase enzymatic hydrolysis 2-4h of 0.3-0.8U/mL,Enzymolysis solution filters，Filtrate is enzyme denaturing 10-15min in boiling water，It is cooled to room temperature，Being evaporated to solid content is more than 20%，Obtain pectin decomposer.
The preparation method that another object of the present invention is to provide the above-mentioned Radix Glycyrrhizae plant beverage improving immunity, comprises the steps:
1) Radix Glycyrrhizae concentrated juice, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer, white sugar, Fructus Momordicae, Mel and citric acid are accurately weighed by formula；
2) first by Radix Glycyrrhizae concentrated juice and concentrated medlar juice Homogeneous phase mixing in uncovered jacketed pan, simultaneously, Fructus Momordicae is crushed to particle diameter 1-3mm and joins jacketed pan, jacketed pan steam pressure is 0.1-0.3MPa, mixes 10-30min, speed of agitator 100-300r/min while stirring, stand 5-10min, filter, obtain mixed concentrated liquid, standby；
3) in blend tank, add the pure water of White Sugar Quality 16-19 times, control temperature 80-90 DEG C, it is sequentially added into white sugar while stirring, Mel and citric acid, speed of agitator 80-120r/min, fully dissolve 20-30min, it is cooled to 75-85 DEG C, insulation, it is sequentially added into mixed concentrated liquid while stirring, fungi extracts, pectin decomposer, modified dietary fiber and bee pollen of maize extract, fully dissolve 20-30min, then through 200-400 order pipeline screen filtration, two sections of plate type heat exchangers are cooled to 1-4 DEG C, pump into sterilizing tapered shaped can, insulation, stand 20-40min, through 0.1-0.3 μm of aseptic filtration, obtain Radix Glycyrrhizae plant beverage semi-finished product；
4) step 3) semi-finished product that obtain are sterilized, namely cooling, sterile filling obtain the Radix Glycyrrhizae plant beverage that can improve immunity.
Beneficial effect:
The present invention is with Radix Glycyrrhizae for primary raw material, the composite Radix Glycyrrhizae concentrated juice of science, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer etc., adopt modern low temperature and biological extraction technology to Feedstock treating, drastically increase the content of material effective component, organic mixing of various functional components, play the synergism of the best, have employed the heating power mixing concentration of unstable concentrated solution simultaneously, cold, the combined removal that heat is muddy, the methods such as aseptic filtration, significantly enhance the local flavor of Radix Glycyrrhizae plant beverage, extend the shelf-life of product, it is prepared for a kind of Radix Glycyrrhizae plant beverage having and can improving human body overall immunity.Concrete principle is:
1. the Radix Glycyrrhizae concentrated juice of the present invention is to prepare through the extract at low temperature technology such as ultrasonic cleaning, freezing, pulverizing, high-pressure pulse electric extraction, ultrasonic wave added microwave extraction, low temperature enzymolysis with Radix Glycyrrhizae for primary raw material, can retain glycyrrhizic acid, enoxolone, liquirtin, isoliquiritin, Angelica Polysaccharide and other antioxidation, volatile, thermal sensitivity effective ingredient to greatest extent.
2. the fungi extracts of the present invention is with repair cell tissue, has antiulcer action, antioxidation, effect of scavenging radical and anti-aging effects, can be obviously improved that human body entirety epidemic disease exempts from power and physical strength reinforcing, the edible fungi that improves gastrointestinal function is prepared through ultrasonic cleaning, high voltage pulse electric field processing and biological enzymolysis, its active constituent content is high, in edible fungi enzymolysis solution, edible fungi polysaccharide content is up to 7.13%, biological activity is higher, foodsafety is high, remaining without pesticide and worm's ovum, effect is more significantly.
3. the concentrated medlar juice of the present invention is by composite to Fructus Lycii, the Radix Astragali and Ganoderma sporophore science, adopt the machining at low temperature method of lyophilization and biological enzymolysis, the active constituent contents such as the concentrated medlar juice lycium barbarum polysaccharide, astragalus polysaccharides and the ganoderan that obtain are higher, functional component is more significantly, is effectively increased the overall immunity of human body.
4. supersound extraction, high-pressure pulse electric are extracted and biological enzymolysis combination of sciences by the modified dietary fiber of the present invention, gained modified dietary fiber soluble fiber cellulose content is high, it is easier to be utilized by probiotic bacteria, improve probiotic bacteria in the growth of intestinal and fertility, increase kind and the quantity of probiotic bacteria flora, reduce large intestine pH value, improve intestine microenvironment；High adsorption capacity, after modified, cellulosic specific surface area increases, and network is enriched, and absorption affinity strengthens, and the organic molecule ability of chelating, absorption cholesterol and bile acids is higher, suppress the human body absorption to them；Ion-exchange capacity strengthens, and to metallic element, particularly heavy metal element adsorption effect is higher, effectively prevent body weight for humans metal poisoning；Retentiveness, dilatancy, thickening property be higher and acid and alkali, alkali, salt impact.Regulate and maintain the resident time of intestinal microbial population, strengthen digestion and the absorbability of intestinal, improve body immunity；Effectively facilitate gastrointestinal peristalsis, slow down and eliminate the untoward reaction such as flatulence, abdominal distention；Powerful embedding effect can prevent environment in beverage (dissolved oxygen, temperature, illumination, the water activity etc.) factor impact on product quality, stabilizes the biological activity of product, extends the shelf-life of product.
5. the bee pollen of maize extract of the present invention is with bee pollen of maize, Glutinous Semen Maydis palpus, defatted corn powder mixture for raw material, successively through ultrasonic cleaning, high-pressure pulse electric and supersound process, biological enzymolysis and prepared, achieve: 1) ultrasonic cleaning can effectively remove bee pollen of maize, Glutinous Semen Maydis must pesticide residues, improve the foodsafety of product；2) high voltage pulse electric field processing, has effectively killed the worm's ovum in bee pollen of maize and Glutinous Semen Maydis palpus and miscellaneous bacteria, for postorder enzymolysis process be normally carried out provide good gnotobasis；3) omnidistance breaking cellular wall, extract (high-pressure pulse electric, ultrasonic, enzymolysis), improve material effective component extraction ratio and nutrient composition content；4) macro-nutrients (protein, starch, fat etc.) both can be converted into little molecule and easily absorb nutrient substance by biological enzymolysis, can realize again the breaking cellular wall to bee pollen of maize further；5) omnidistance employing extract at low temperature technology, effectively prevent reproducibility effective ingredient and the loss of volatile fragrant components (in Glutinous Semen Maydis palpus), carry and alcohol extraction through water, extract contains the bee pollen of maize of high level and the effective ingredient of Glutinous Semen Maydis palpus, the human body absorption to nutrient substance can be effectively facilitated, improve human body overall immunity, also has strong natural corn fragrance simultaneously, significantly improve the corn fragrance of material effective component content and finished product, enhance appetite, improve the uptake ratio of nutritional labeling, digestibility and absorbance.
6. the pectin decomposer of the present invention has natural, soft agreeable to the taste tart flavour and significant antagonistic property, especially escherichia coli inhibitory action is the strongest, also there is stronger non-oxidizability simultaneously, can act on when concentration reaches 0.01%, to superoxide radical, hydroxy radical and DPPH free radical all show very strong elimination effect, when concentration reaches 1.0%, eradicating efficacy reaches 100%, the science of described pectin decomposer is composite, the palatability of Radix Glycyrrhizae plant beverage can be effectively improved, most importantly its significant antagonistic property and non-oxidizability avoid oxidation and the microorganism encroach of effective ingredient in raw material, stabilize and increase the biological activity of Radix Glycyrrhizae plant beverage, extend the shelf-life of product.
7. can to improve the preparation method technique of immunity Radix Glycyrrhizae plant beverage simple for the present invention, easy to operate, by unstable Radix Glycyrrhizae concentrated juice and the mixing concentration of concentrated medlar juice heat-flash power, not only increase the mixing local flavor of Radix Glycyrrhizae, Fructus Lycii, create strong fragrance, and eliminate coarse sludge therein, adopt the cold sludge that low temperature (1-4 DEG C) stands, has been filtered to remove in beverage simultaneously, cold and heat succeed each other process, beverage liquid phase is more stable, significantly enhances the local flavor of Radix Glycyrrhizae plant beverage, extends the shelf-life of product.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, under the premise without departing substantially from spirit and scope of the present invention, the various changes or the changes that carry out the material component in these embodiments and consumption fall within protection scope of the present invention.
Prepared by embodiment 1 raw material
1. the preparation of Radix Glycyrrhizae concentrated juice: the preparation method of described Radix Glycyrrhizae concentrated juice, comprise the steps: Radix Glycyrrhizae tabellae, Flos Lonicerae and Flos Caryophylli to be respectively put in the ultrasonic washing unit equipped with 0.4% sodium bicarbonate solution and clean 7min in 200W, 40KHz, drain, 8:2:1.5 Homogeneous phase mixing in mass ratio, then in-22 DEG C of freezing 8min；It is crushed to particle diameter 0.5mm immediately；Put in container and add the water of 4 times of weight, obtaining mixed material, being 4.5 with breast acid for adjusting pH value, at electric field intensity 30kV/cm under room temperature, burst length 400 μ s, carry out high voltage pulse electric field processing when pulse frequency 300Hz；Then heat to 35 DEG C, insulation, carry out microwave extraction when power 250W, wherein, each microwave exposure total time 70s, carry out compartment irradiation: irradiation 10s, interval 10s, such irradiation 10 times, simultaneously at power 250W, carries out ultrasonic assistant extraction when frequency 35KHz；It is continuously heating to 40-50 DEG C, insulation, it is subsequently adding the compound enzyme enzymolysis 40min of mixed material gross weight 3%, filters, obtain filtrate；Filtering residue 78 DEG C of water of 2 times of weight rinse 3 times, and rinsing liquid merges with filtrate, Homogeneous phase mixing, and being evaporated to solid content is more than 20%, obtains Radix Glycyrrhizae concentrated juice；
Described compound enzyme is by pectase, cellulase, tannase, amylase and protease 5:4:3:2:1.5 Homogeneous phase mixing in mass ratio.
2. the preparation of fungi extracts: the preparation method of described fungi extracts comprises the steps: Hericium erinaceus (Bull. Ex Fr.) Pers., Semen Ginkgo and Auricularia to be respectively put in the ultrasonic washing unit equipped with 0.4% sodium bicarbonate solution and cleans 8min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, in mass ratio 12:5:2.5 Homogeneous phase mixing, then in-23 DEG C of freezing 20min, pulverizing immediately, ground product particle diameter is 0.5mm；The water adding ground product quality 4 times obtains mixture, adjusting mixture pH is 5.2, at electric field intensity 40kV/cm under room temperature, burst length 600 μ s, carry out high voltage pulse electric field processing when pulse frequency 300Hz, it is warming up to 50 DEG C, the mixed enzyme enzymolysis of addition mixture weight 0.8% 5 hours, obtains edible fungi enzymolysis solution, filters, filtrate is more than 30% through being evaporated to solid content, obtains fungi extracts；
Described mixed enzyme parts by weight consist of: papain 25 parts, bromelain 25 parts, glucanase 12 parts, xylanase 12 parts, pentosanase 12 parts, ficin 8 parts, midrange thermal stable amylase 8 parts, pectase 8 parts, tannase 4 parts；
In the edible fungi enzymolysis solution of above-mentioned preparation, edible fungi polysaccharide content is 7.13%.
3. the preparation of concentrated medlar juice: the preparation method of described concentrated medlar juice, comprises the steps: Fructus Lycii, the Radix Astragali, Ganoderma sporophore 11:2:1.5 Homogeneous phase mixing in mass ratio, and after flushing, quick freeze is to-35 DEG C；Sublimation drying, vacuum 450Pa, it is dried to moisture 10%；It is crushed to particle diameter in-6 DEG C and obtains ground product at below 1.5mm；Add the aqueous citric acid solution of ground product weight 5 times, the pH value controlling solution is 4.5, add the compound enzyme of mixture weight 0.8%, described compound enzyme is made up of acid pectase, neutral protease and cellulase, the weight ratio of three kinds of enzymes is 2.5: 1: 1.5, hydrolysis temperature is 45 DEG C, and enzymolysis time is 2hr；Afterwards pH value being adjusted to 5.5, temperature is raised to 55 DEG C, simultaneously add the pectase of mixture weight 0.4%, 0.6% glucanase, action time is 3hr；Enzymolysis solution is 8 DEG C of filtrations；Filtrate freeze concentration obtains compound concentrated medlar juice.
4. the preparation of modified dietary fiber: the preparation method of described modified dietary fiber, comprise the following steps: by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber 9:5:4:3 Homogeneous phase mixing in mass ratio, add the water of its quality 5 times, room temperature 250W, 40KHz condition supersound extraction 12min, then at electric field intensity 30kV/cm, burst length 500 μ s, carries out high-pressure pulse electric extraction when pulse frequency 300Hz；It is 5.2 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.2%, in 50 DEG C of enzymolysis 35min；Enzymolysis solution concentrating under reduced pressure, lyophilization, low-temperature grinding to particle diameter are that namely 0.2mm obtains modified dietary fiber；
Described enzyme is xylanase, cellulase, laccase, pectase, tannase 8:4:3:3:2 Homogeneous phase mixing in mass ratio.
5. the preparation of bee pollen of maize extract: the preparation method of described bee pollen of maize extract, comprise the steps: that 50:4:3 weighs bee pollen of maize respectively in mass ratio, Glutinous Semen Maydis must with defatted corn powder, first by bee pollen of maize, Glutinous Semen Maydis must be put in the ultrasonic washing unit equipped with 0.4% sodium bicarbonate solution respectively, in 200W under room temperature, 40KHz cleans 4min, drain, then mix with defatted corn powder, add the water of former bee pollen of maize quality 4 times, it is 5 with breast acid for adjusting pH value, in electric field intensity 30kV/cm, burst length 400 μ s, high voltage pulse electric field processing is carried out when pulse frequency 300Hz, then room temperature 400W, 40KHz condition supersound process 12min；Being warming up to 45 DEG C, add the enzyme preparation of former bee pollen of maize quality 5%, stir, enzymolysis 18min, enzymolysis solution liquid filters through 200 eye mesh screens, and namely filtrate obtain bee pollen of maize extract through ultrafiltration, concentrating under reduced pressure, lyophilization；
The quality group of described enzyme preparation becomes: protease: saccharifying enzyme: pectase: cellulase: lipase=12:8:5:4:2.
6. the preparation of pectin decomposer: the preparation method of described pectin decomposer, comprise the steps: fresh Fructus Crataegi marc, Pericarpium Vitis viniferae, pomace, megasse to be respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 8min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, in mass ratio 8:4:3:3 Homogeneous phase mixing, then in-23 DEG C of freezing 20min；Pulverizing immediately, particle diameter is 0.5mm；nullAdd the deionized water of ground product quality 4 times，Mix homogeneously，It is 2.5 with Fructus Citri Limoniae acid for adjusting pH value，At electric field intensity 40kV/cm under room temperature，Burst length 500 μ s,High voltage pulse electric field processing is carried out when pulse frequency 300Hz，Then normal pressure boils 40min，Bag filter，The filtering residue filtrate normal pressure of its quality 2 times boils 50min,Bag filter，Merging filtrate，Kieselguhr filters，Filtrate is made to clarify，Being evaporated to solid content at 75 DEG C is 8%，It is rapidly cooled to room temperature，Add 95% ethanol that pH value is 2.5 of 1.5 times of volumes of concentrated solution，Stand 25min,Pectin is made to be precipitated out，4000r/min is centrifuged 8min，Reclaim precipitation，With the dehydrated alcohol eluting of 2 times of volumes，Centrifugal，Reclaim precipitation，So operation 2 times，What obtain is deposited in 55 DEG C of vacuum drying 2.5h,Obtain pectin，Add 45 DEG C of water of pectin quality 4.5 times，Stirring，Homogeneous phase mixing，Insulation，It is 5.0 with breast acid for adjusting pH value，Add the pectinase enzymatic hydrolysis 3h of 0.5U/mL,Enzymolysis solution filters，Filtrate is enzyme denaturing 10-15min in boiling water，It is cooled to room temperature，Being evaporated to solid content is more than 20%，Obtain pectin decomposer.
Radix Glycyrrhizae concentrated juice that following embodiment 2-6 uses, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer are embodiment 1 and prepare, and other is commercial commodity.
Embodiment 2
A kind of Radix Glycyrrhizae plant beverage improving immunity, is mainly prepared by the raw material of following parts by weight:
Radix Glycyrrhizae concentrated juice 12 parts, fungi extracts 20 parts, concentrated medlar juice 10 parts, modified dietary fiber 10 parts, bee pollen of maize extract 20 parts, pectin decomposer 5 parts；
The described Radix Glycyrrhizae plant beverage improving immunity also includes the adjuvant of following parts by weight: white sugar 150 parts, Fructus Momordicae 13 parts, Mel 9 parts, citric acid 2 parts；
The preparation method of the above-mentioned Radix Glycyrrhizae plant beverage improving immunity, comprises the steps:
1) Radix Glycyrrhizae concentrated juice, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer, white sugar, Fructus Momordicae, Mel and citric acid are accurately weighed by formula；
2) first by Radix Glycyrrhizae concentrated juice and concentrated medlar juice Homogeneous phase mixing in uncovered jacketed pan, simultaneously, Fructus Momordicae is crushed to particle diameter 2mm and joins jacketed pan, jacketed pan steam pressure is 0.2MPa, mixes 20min, speed of agitator 200r/min while stirring, stand 8min, filter, obtain mixed concentrated liquid, standby；
3) in blend tank, add the pure water of White Sugar Quality 18 times, control temperature 85 DEG C, it is sequentially added into white sugar while stirring, Mel and citric acid, speed of agitator 100r/min, fully dissolve 25min, it is cooled to 80 DEG C, insulation, it is sequentially added into mixed concentrated liquid while stirring, fungi extracts, pectin decomposer, modified dietary fiber and bee pollen of maize extract, fully dissolve 25min, then through 300 order pipeline screen filtrations, two sections of plate type heat exchangers are cooled to 2 DEG C, pump into sterilizing tapered shaped can, insulation, stand 30min, through 0.2 μm of aseptic filtration, obtain Radix Glycyrrhizae plant beverage semi-finished product；
4) step 3) semi-finished product that obtain are sterilized, namely cooling, sterile filling obtain the Radix Glycyrrhizae plant beverage that can improve immunity.
Embodiment 3
A kind of Radix Glycyrrhizae plant beverage improving immunity, is mainly prepared by the raw material of following parts by weight:
Radix Glycyrrhizae concentrated juice 3 parts, fungi extracts 10 parts, concentrated medlar juice 5 parts, modified dietary fiber 5 parts, bee pollen of maize extract 10 parts, pectin decomposer 2 parts；
The described Radix Glycyrrhizae plant beverage improving immunity also includes the adjuvant of following parts by weight: white sugar 50 parts, Fructus Momordicae 8 parts, Mel 3 parts, citric acid 1 part；
The preparation method of the above-mentioned Radix Glycyrrhizae plant beverage improving immunity, comprises the steps:
1) Radix Glycyrrhizae concentrated juice, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer, white sugar, Fructus Momordicae, Mel and citric acid are accurately weighed by formula；
2) first by Radix Glycyrrhizae concentrated juice and concentrated medlar juice Homogeneous phase mixing in uncovered jacketed pan, simultaneously, Fructus Momordicae is crushed to particle diameter 1mm and joins jacketed pan, jacketed pan steam pressure is 0.1MPa, mixes 10min, speed of agitator 100r/min while stirring, stand 5min, filter, obtain mixed concentrated liquid, standby；
3) in blend tank, add the pure water of White Sugar Quality 16 times, control temperature 80 DEG C, it is sequentially added into white sugar while stirring, Mel and citric acid, speed of agitator 80r/min, fully dissolve 20min, it is cooled to 75 DEG C, insulation, it is sequentially added into mixed concentrated liquid while stirring, fungi extracts, pectin decomposer, modified dietary fiber and bee pollen of maize extract, fully dissolve 20min, then through 200 order pipeline screen filtrations, two sections of plate type heat exchangers are cooled to 1 DEG C, pump into sterilizing tapered shaped can, insulation, stand 20min, through 0.1 μm of aseptic filtration, obtain Radix Glycyrrhizae plant beverage semi-finished product；
4) step 3) semi-finished product that obtain are sterilized, namely cooling, sterile filling obtain the Radix Glycyrrhizae plant beverage that can improve immunity.
Embodiment 4
A kind of Radix Glycyrrhizae plant beverage improving immunity, is mainly prepared by the raw material of following parts by weight:
Radix Glycyrrhizae concentrated juice 20 parts, fungi extracts 30 parts, concentrated medlar juice 15 parts, modified dietary fiber 15 parts, bee pollen of maize extract 30 parts, pectin decomposer 8 parts；
The described Radix Glycyrrhizae plant beverage improving immunity also includes the adjuvant of following parts by weight: white sugar 250 parts, Fructus Momordicae 18 parts, Mel 15 parts, citric acid 3 parts；
The preparation method of the above-mentioned Radix Glycyrrhizae plant beverage improving immunity, comprises the steps:
1) Radix Glycyrrhizae concentrated juice, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer, white sugar, Fructus Momordicae, Mel and citric acid are accurately weighed by formula；
2) first by Radix Glycyrrhizae concentrated juice and concentrated medlar juice Homogeneous phase mixing in uncovered jacketed pan, simultaneously, Fructus Momordicae is crushed to particle diameter 3mm and joins jacketed pan, jacketed pan steam pressure is 0.3MPa, mixes 30min, speed of agitator 300r/min while stirring, stand 10min, filter, obtain mixed concentrated liquid, standby；
3) in blend tank, add the pure water of White Sugar Quality 19 times, control temperature 90 DEG C, it is sequentially added into white sugar while stirring, Mel and citric acid, speed of agitator 120r/min, fully dissolve 30min, it is cooled to 85 DEG C, insulation, it is sequentially added into mixed concentrated liquid while stirring, fungi extracts, pectin decomposer, modified dietary fiber and bee pollen of maize extract, fully dissolve 30min, then through 400 order pipeline screen filtrations, two sections of plate type heat exchangers are cooled to 4 DEG C, pump into sterilizing tapered shaped can, insulation, stand 40min, through 0.3 μm of aseptic filtration, obtain Radix Glycyrrhizae plant beverage semi-finished product；
4) step 3) semi-finished product that obtain are sterilized, namely cooling, sterile filling obtain the Radix Glycyrrhizae plant beverage that can improve immunity.
Embodiment 5
A kind of Radix Glycyrrhizae plant beverage improving immunity, is mainly prepared by the raw material of following parts by weight:
Radix Glycyrrhizae concentrated juice 8 parts, fungi extracts 15 parts, concentrated medlar juice 8 parts, modified dietary fiber 8 parts, bee pollen of maize extract 15 parts, pectin decomposer 4 parts；
The described Radix Glycyrrhizae plant beverage improving immunity also includes the adjuvant of following parts by weight: white sugar 100 parts, Fructus Momordicae 12 parts, Mel 8 parts, citric acid 1.5 parts；
The preparation method of the above-mentioned Radix Glycyrrhizae plant beverage improving immunity, comprises the steps:
1) Radix Glycyrrhizae concentrated juice, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer, white sugar, Fructus Momordicae, Mel and citric acid are accurately weighed by formula；
2) first by Radix Glycyrrhizae concentrated juice and concentrated medlar juice Homogeneous phase mixing in uncovered jacketed pan, simultaneously, Fructus Momordicae is crushed to particle diameter 1mm and joins jacketed pan, jacketed pan steam pressure is 0.3MPa, mixes 10min, speed of agitator 300r/min while stirring, stand 5min, filter, obtain mixed concentrated liquid, standby；
3) in blend tank, add the pure water of White Sugar Quality 16 times, control temperature 90 DEG C, it is sequentially added into white sugar while stirring, Mel and citric acid, speed of agitator 80r/min, fully dissolve 30min, it is cooled to 75 DEG C, insulation, it is sequentially added into mixed concentrated liquid while stirring, fungi extracts, pectin decomposer, modified dietary fiber and bee pollen of maize extract, fully dissolve 30min, then through 200 order pipeline screen filtrations, two sections of plate type heat exchangers are cooled to 1 DEG C, pump into sterilizing tapered shaped can, insulation, stand 40min, through 0.1 μm of aseptic filtration, obtain Radix Glycyrrhizae plant beverage semi-finished product；
4) step 3) semi-finished product that obtain are sterilized, namely cooling, sterile filling obtain the Radix Glycyrrhizae plant beverage that can improve immunity.
Embodiment 6
A kind of Radix Glycyrrhizae plant beverage improving immunity, is mainly prepared by the raw material of following parts by weight:
Radix Glycyrrhizae concentrated juice 16 parts, fungi extracts 25 parts, concentrated medlar juice 12 parts, modified dietary fiber 12 parts, bee pollen of maize extract 25 parts, pectin decomposer 6 parts；
The described Radix Glycyrrhizae plant beverage improving immunity also includes the adjuvant of following parts by weight: white sugar 200 parts, Fructus Momordicae 15 parts, Mel 10 parts, citric acid 2.5 parts；
The preparation method of the above-mentioned Radix Glycyrrhizae plant beverage improving immunity, comprises the steps:
1) Radix Glycyrrhizae concentrated juice, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer, white sugar, Fructus Momordicae, Mel and citric acid are accurately weighed by formula；
2) first by Radix Glycyrrhizae concentrated juice and concentrated medlar juice Homogeneous phase mixing in uncovered jacketed pan, simultaneously, Fructus Momordicae is crushed to particle diameter 3mm and joins jacketed pan, jacketed pan steam pressure is 0.1MPa, mixes 30min, speed of agitator 100r/min while stirring, stand 10min, filter, obtain mixed concentrated liquid, standby；
3) in blend tank, add the pure water of White Sugar Quality 16 times, control temperature 90 DEG C, it is sequentially added into white sugar while stirring, Mel and citric acid, speed of agitator 80r/min, fully dissolve 30min, it is cooled to 75 DEG C, insulation, it is sequentially added into mixed concentrated liquid while stirring, fungi extracts, pectin decomposer, modified dietary fiber and bee pollen of maize extract, fully dissolve 30min, then through 200 order pipeline screen filtrations, two sections of plate type heat exchangers are cooled to 4 DEG C, pump into sterilizing tapered shaped can, insulation, stand 20min, through 0.3 μm of aseptic filtration, obtain Radix Glycyrrhizae plant beverage semi-finished product；
4) step 3) semi-finished product that obtain are sterilized, namely cooling, sterile filling obtain the Radix Glycyrrhizae plant beverage that can improve immunity.
Embodiment 7 present invention can improve the sensory evaluation test of the Radix Glycyrrhizae plant beverage of immunity
Invite 24 personnel that the Radix Glycyrrhizae plant beverage of improved immunity prepared by embodiment of the present invention 2-6 is judged with commercially available two kinds of Radix Glycyrrhizae plant beverages, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), and marking personnel independently carry out, and are independent of each other, and judges result with guarantee accurate.Having added up judging result, equal score value takes approximation, retains integer, is specifically shown in table 1:
Table 1 sensory evaluation statistical result
Note: show significant difference (P < 0.05) with the different lowercase alphabet of mark in a line, the different capitalization of mark represents difference extremely notable (P < 0.01), indicates same letter and represents difference not notable (P > 0.05).
Result above shows, from outward appearance, quality, local flavor and mouthfeel, any one will be substantially better than commercially available Radix Glycyrrhizae plant beverage to the Radix Glycyrrhizae plant beverage of improved immunity prepared by the present invention, particularly local flavor is fabulous, highlight the Special Mixed fragrance of Radix Glycyrrhizae, Fructus Lycii, bee pollen of maize, also be adapted for different age group, the consumer of different hierarchy of consumption eats simultaneously.
Embodiment 8 present invention can improve the storage stability of the Radix Glycyrrhizae plant beverage of immunity
With the same specification Radix Glycyrrhizae plant beverage of Radix Glycyrrhizae plant beverage and the commercially available identical date of manufacture of the improved immunity of the embodiment of the present invention 2 preparation for subjects, under identical storage condition (room temperature, ventilation, lucifuge), detect the sanitary indexs such as total plate count, escherichia coli, pathogenic bacterium, mycete, yeast, residual pesticide, heavy metal by fruit-vegetable juice beverage sanitary standard GB19297-2003, result is table 2 such as:
Table 2 stores 12 months sanitary index testing results
Result above shows: when commercially available Radix Glycyrrhizae plant beverage stored to 12 months, although its total plate count and coliform are qualified, but content of microorganisms is higher；The Radix Glycyrrhizae plant beverage pollution microbes kind that the present invention can improve immunity is few, every Testing index stores and is superior to national standard in 12 months, and the prolongation along with the time of storage, content of microorganisms propagation is slowly, show that the present invention can improve the microbial stability that the Radix Glycyrrhizae plant beverage of immunity is excellent, simultaneously the present invention can improve the Radix Glycyrrhizae plant beverage preparation process of immunity exotic plant raw material be have employed ultrasonic cleaning, effectively reduce agriculture residual, the content of toxin and heavy metal ion, although commercially available Radix Glycyrrhizae plant beverage is qualified, but there is bigger food safety hidden danger, human body be caused the serious harm of accumulation property by long-term edible meeting.
After testing, Radix Glycyrrhizae plant beverage prepared by embodiment of the present invention 3-6 stores 12 months with embodiment 2 sanitary index testing result difference not quite, has excellent foodsafety and microbial stability equally.
Embodiment 9 present invention can improve the Radix Glycyrrhizae plant beverage mouse intestinal performance test of immunity
Choosing common Kunming white mice 60, male and female half and half, 18-20g, conventional word is supported.Therefrom random choose 40,9:00 gavage lincomycin hydrochloride 0.2mL every morning (20mg)/only, other as a control group, every day same time gavage equivalent sterile saline, continuous one week, prepare the mouse model of alteration of intestinal flora.Model group mouse diet declines, and dead and phenomenon of significantly suffering from diarrhoea does not occur, arranges soft excrement, and profile normal aqueous divides more, and bedding and padding are moist.By 40 alteration of intestinal flora mices, being randomly divided into 2 groups, one group 20 is only used as treatment group, and every day gavage Radix Glycyrrhizae plant beverage 5ml/ of the present invention, another 20 are only used as natural recovering group, every day same time gavage equivalent sterile saline, continuous two weeks.21 days whole experimental periods, observing growth and the defecation situation of white mice every day, weigh in the 8th, 21 days mices to probiotic tablet treatment group and natural recovering group, calculate each group of weight average rate of increase, result is table 3 such as；Within every 5 days, surveying each group of stool in mice escherichia coli quantity, calculate average, result is table 4 such as.Take stool in mice and be about 0.1g, in aseptic operating platform, add 3 beades (adding 0.5mL diluent with 0.1g excrement sample), dilute and inoculate maconkey agar culture medium, calculate every gram of coliform count wet in just.
Table 3 mice Gain weight
| Packet | Average starting weight (g/ is only) | Average end weight (g/ is only) | Average rate of increase (%) |
| Natural recovering group | 20.69±1.33 | 27.34±1.59 | 32.14a |
| Treatment group | 20.41±1.45 | 32.58±1.62 | 59.63b |
The situation of coliform count in table 4 stool in mice
Radix Glycyrrhizae plant beverage treatment group Mouse Weight average rate of increase (59.63%) is significantly higher than natural recovering group (32.14%)；Feed beverage rear intestinal escherichia coli quantity to be remarkably decreased, reduce by 82.62%, it is substantially less than natural recovering group (24.78%), show that the present invention can improve the Radix Glycyrrhizae plant beverage of immunity and can promote proliferation of probiotics field planting rapidly in white mice intestinal, form dominant microflora, and effectively suppress the growth and breeding of the pathogen such as escherichia coli, and resident time is long, continues, effectively improves intestinal performance.
It should be understood that the Radix Glycyrrhizae plant beverage of improved immunity prepared by embodiment of the present invention 3-6 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect diversity.
Embodiment 10 present invention can improve the impact on immunity of organisms of the Radix Glycyrrhizae plant beverage of immunity
1 experiment purpose
Test (mouse forced swimming) by exercise tolerance, verify the raising immunity of Radix Glycyrrhizae plant beverage of the present invention, antifatigue effect.
2 experiment materials and reagent
2.1 for reagent thing:
Commercially available Radix Glycyrrhizae plant beverage (G1)；Commercially available Radix Glycyrrhizae plant beverage (G2)；Radix Glycyrrhizae plant beverage (G3-G7) prepared by embodiment of the present invention 2-6.
2.2 reagent:
Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), Nanjing Chemistry Reagent Co., Ltd.；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..
3. laboratory animal
ICR mice, ♂, cleaning grade, body weight 18-22g, Yangzhou University's comparative medicine center provide, the quality certification number: SCXK (Soviet Union) 2,007 0001, the free diet of mice during experiment.
4. key instrument
Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof company；Water-bath: DK-S26 type, the grand experimental facilities company limited of upper Nereid；Electronic scale: BS224S type, Sartorius company；Stopwatch, thermometer
5. experiment packet
5.1 dosage packets and given the test agent give the time and at random mice are divided into 8 groups, often group 10,1st group to the 7th group medicine giving G1～G7 respectively, 8th group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.
5.2 sample preparations the 1st group to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank group: distilled water 150ml.
6. experimental technique
After 6.1 swimming with a load attached to the body experiment last administration 30min, putting mice in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, and record mice swimming started to the dead time, as mice swimming time.
After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, eyeball blood sampling 0.5mL (being not added with anticoagulant) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, after hemopexis, the centrifugal 15min of 2000r/min, takes serum and send clinical laboratory of Lianyungang First People's Hospital to detect.
After the mensuration last administration 30min of 6.3 hepatic glycogen, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical dislocation puts to death mice, clean with normal saline, and with, after filter paper suck dry moisture, accurately weighing liver 100mg, hepatic glycogen detection kit detection Mouse Liver glycogen content.
Blood sampling after the mensuration last administration 30min of 6.4 blood lactase acid, does not then bear a heavy burden and stops after the water went swimming 10min that temperature is 30 DEG C.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid after rest 20min after swimming, after swimming, fully vibration smudge cells lactic acid instrument measures immediately.(blood lactase acid area under curve=5 × (after front blood lactase acid value+3 × swimming of swimming the blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min)
7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value
8. statistical method experimental data is usedRepresent, adopt t inspection to compare between organizing
9. experimental result
9.1 present invention can improve the impact on Mouse Weight of the Radix Glycyrrhizae plant beverage of immunity
Each group mice is after giving G1～G7 medicine, before, in, post-weight is shown in shown in following table respectively, and each original body mass organizing mice and weightening finish body weight compare equal no difference of science of statistics (P > 0.05) with matched group, it was shown that G1～G7 medicine is all without obvious toxicity.Experimental result refers to table 5.
The original body mass of table 5 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight
9.2 present invention can improve the impact on the mice burden swimming time of the Radix Glycyrrhizae plant beverage of immunity
After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, the mice burden swimming time can be obviously prolonged, there is significant difference (P < 0.05), Radix Glycyrrhizae plant beverage G3～G7 medicine of the present invention compares with blank group, can significantly extend the mice burden swimming time, there is pole significant difference (P < 0.01), and be substantially better than G1～G2 medicine.The results detailed in Table 6.
Table 6 can improve the impact on the mice burden swimming time of the Radix Glycyrrhizae plant beverage of immunity
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
9.3 present invention can improve the Radix Glycyrrhizae plant beverage of immunity to the impact of blood lactase acid before and after mouse movement
After per os gives the Radix Glycyrrhizae plant beverage that the mice present invention can improve immunity, the present invention can improve the Radix Glycyrrhizae plant beverage G3～G7 medicine of immunity and blood lactase acid area under curve after mouse movement is compared with matched group have significant difference (P < 0.05), decrease though G1～G2 medicine group Mouse Blood lactic acid area under curve compares with matched group, but and no difference of science of statistics (P > 0.05).Result is in Table 7.
Table 7 present invention can improve the Radix Glycyrrhizae plant beverage of immunity to the impact of blood lactase acid level before and after mouse movement
" * " p < 0.05vs blank；
9.4 present invention can improve the impact on Mouse Liver glycogen of the Radix Glycyrrhizae plant beverage of immunity
After per os gives mice G1～G7 medicine, G1～G2 medicine compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), the present invention can improve the Radix Glycyrrhizae plant beverage G3～G7 medicine of immunity and compare with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is substantially better than G1～G2 medicine.The results detailed in Table 8.
Table 8 present invention can improve the impact on Mouse Liver glycogen content of the Radix Glycyrrhizae plant beverage of immunity
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
9.5 present invention can improve the impact on mice serum carbamide of the Radix Glycyrrhizae plant beverage of immunity
After per os gives mice G1～G7 medicine, G1～G2 medicine group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), the present invention can improve the Radix Glycyrrhizae plant beverage G3～G7 medicine of immunity and compare with blank group, after mouse movement, serum urea content all has obvious reduction, there is pole significant difference (P < 0.01), and be substantially better than G1～G2 medicine.The results detailed in Table 9.
Table 9 present invention can improve the impact on mice serum urea content of the Radix Glycyrrhizae plant beverage of immunity
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
10. experiment conclusion
This experiment mainly through mice burden swimming test, simultaneously detect Mouse Liver glycogen deposit observe the present invention can improve immunity Radix Glycyrrhizae plant beverage improve immunity, resisting fatigue effect.Preliminary Results shows below:
1, G3～G7 of the present invention can improve the Radix Glycyrrhizae plant beverage of immunity and all can extend the mice burden swimming time (P < 0.01), and effect is substantially better than the Radix Glycyrrhizae plant beverage of other G1～G2.
2, biochemistry detection aspect shows, the each dosage group of Radix Glycyrrhizae plant beverage of immunity of can improving G3～G7 of the present invention all can reduce move after lactic acid content produced by glucose anerobic glycolysis in mice serum, compare with matched group and have significant difference (P < 0.05), and although the Radix Glycyrrhizae plant beverage of other G1～G2 also can reduce after motion lactic acid content produced by glucose anerobic glycolysis in mice serum, but compare with matched group, no difference of science of statistics (P > 0.05)；
3, G3～G7 of the present invention can improve each dosage group of Radix Glycyrrhizae plant beverage of immunity and all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and effect is substantially better than the Radix Glycyrrhizae plant beverage of other G1～G2；
4, metabolic arthritis model finds, G3～G7 of the present invention can improve the Radix Glycyrrhizae plant beverage of immunity can significantly reduce the content (P < 0.01) of urea in serum after mice is swum, and effect is substantially better than other G1～G2 Radix Glycyrrhizae plant beverage；
11. conclusion
Above-mentioned experiment proves that Radix Glycyrrhizae plant beverage of the present invention can significantly improve immunity of organisms, improve muscle power and the endurance of mice, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to alleviating the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.
Claims (10)
1. can improve a Radix Glycyrrhizae plant beverage for immunity, mainly be prepared by the raw material of following parts by weight: Radix Glycyrrhizae concentrated juice 3-20 part, fungi extracts 10-30 part, concentrated medlar juice 5-15 part, modified dietary fiber 5-15 part, bee pollen of maize extract 10-30 part, pectin decomposer 2-8 part；Also include the adjuvant of following parts by weight: white sugar 50-250 part, Fructus Momordicae 8-18 part, Mel 3-15 part, citric acid 1-3 part；
Described Radix Glycyrrhizae concentrated juice is to prepare through ultrasonic cleaning, freezing, pulverizing, high-pressure pulse electric extraction, ultrasonic wave added microwave extraction and low temperature enzymolysis with Radix Glycyrrhizae for primary raw material；
Described fungi extracts is to be prepared through ultrasonic cleaning, high voltage pulse electric field processing and biological enzymolysis by edible fungi.
2. can improve the Radix Glycyrrhizae plant beverage of immunity as claimed in claim 1, it is characterized in that, mainly prepared by the raw material of following parts by weight: Radix Glycyrrhizae concentrated juice 8-16 part, fungi extracts 15-25 part, concentrated medlar juice 8-12 part, modified dietary fiber 8-12 part, bee pollen of maize extract 15-25 part, pectin decomposer 4-6 part；Also include the adjuvant of following parts by weight: white sugar 100-200 part, Fructus Momordicae 12-15 part, Mel 8-10 part, citric acid 1.5-2.5 part.
3. can improve the Radix Glycyrrhizae plant beverage of immunity as claimed in claim 1, it is characterized in that, the preparation method of described Radix Glycyrrhizae concentrated juice, comprise the steps: Radix Glycyrrhizae tabellae, Flos Lonicerae and Flos Caryophylli to be respectively put in the ultrasonic washing unit equipped with 0.3-0.5% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz, drain, 6-10:1-3:1-2 Homogeneous phase mixing in mass ratio, then in-18-23 DEG C of freezing 5-10min；It is crushed to particle diameter 0.3-1mm immediately；Put in container and add the water of 3-5 times of weight, obtaining mixed material, being 3.5-5.5 with breast acid for adjusting pH value, at electric field intensity 25-35kV/cm under room temperature, burst length 300-500 μ s, carry out high voltage pulse electric field processing when pulse frequency 200-300Hz；Then heat to 30-40 DEG C, insulation, microwave extraction is carried out when power 150-300W, wherein, each microwave exposure total time 60-80s, compartment irradiation is carried out: irradiation 10s, interval 10s, such irradiation 10 times, simultaneously at power 200-300W, carries out ultrasonic assistant extraction when frequency 30-40KHz；It is continuously heating to 40-50 DEG C, insulation, it is subsequently adding the compound enzyme enzymolysis 30-50min of mixed material gross weight 2-5%, filters, obtain filtrate；Filtering residue rinses 3 times with 1-3 times of weight 76-80 DEG C of water, and rinsing liquid merges with filtrate, Homogeneous phase mixing, and being evaporated to solid content is more than 20%, obtains Radix Glycyrrhizae concentrated juice；
Described compound enzyme is by pectase, cellulase, tannase, amylase and protease 3-7:3-5:2-4:1-3:1-2 Homogeneous phase mixing in mass ratio.
4. can improve the Radix Glycyrrhizae plant beverage of immunity as claimed in claim 1, it is characterized in that, the preparation method of described fungi extracts, comprise the steps: Hericium erinaceus (Bull. Ex Fr.) Pers., Semen Ginkgo and Auricularia to be respectively put in the ultrasonic washing unit equipped with 0.3-0.5% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 11-13:4-6:2-3 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min, pulverizing immediately, ground product particle diameter is 0.2-1mm；The water adding ground product quality 3-5 times obtains mixture, adjusting mixture pH is 4.5-6, at electric field intensity 35-45kV/cm under room temperature, burst length 500-700 μ s, carry out high voltage pulse electric field processing when pulse frequency 200-400Hz, it is warming up to 45～55 DEG C, the mixed enzyme enzymolysis of addition mixture weight 0.5-1.2% 4～6 hours, obtains edible fungi enzymolysis solution, filters, filtrate is more than 30% through being evaporated to solid content, obtains fungi extracts；
Described mixed enzyme parts by weight consist of: papain 20-30 part, bromelain 20-30 part, glucanase 10-15 part, xylanase 10-15 part, pentosanase 10-15 part, ficin 5-10 part, midrange thermal stable amylase 5-10 part, pectase 5-10 part, tannase 3-5 part.
5. can improve the Radix Glycyrrhizae plant beverage of immunity as claimed in claim 1, it is characterized in that, the preparation method of described concentrated medlar juice, comprises the steps: Fructus Lycii, the Radix Astragali, Ganoderma sporophore 10-12:1-3:1-2 Homogeneous phase mixing in mass ratio, and after flushing, quick freeze is to-40--30 DEG C；Sublimation drying, vacuum 400-500Pa, it is dried to moisture < 12%；It is crushed to particle diameter in-10--2 DEG C and obtains ground product at below 1.5mm；Add the aqueous citric acid solution of ground product weight 3-6 times, the pH value controlling solution is 4-5, add the compound enzyme of mixture weight 0.5-1%, described compound enzyme is made up of acid pectase, neutral protease and cellulase, the weight ratio of three kinds of enzymes is 2.5: 1: 1.5, hydrolysis temperature is 40-48 DEG C, and enzymolysis time is 1.5-2hr；Afterwards pH value being adjusted to 5.1-5.6, temperature is raised to 50-60 DEG C, adds the glucanase of the pectase of mixture weight 0.2-0.5%, 0.2-1% simultaneously, and action time is 2.5-3hr；Enzymolysis solution is 5-10 DEG C of filtration；Filtrate freeze concentration obtains compound concentrated medlar juice.
6. can improve the Radix Glycyrrhizae plant beverage of immunity as claimed in claim 1, it is characterized in that, the preparation method of described modified dietary fiber comprises the following steps: by inulin, apple fiber, Herba avenae fatuae fiber, Semen Tritici aestivi fiber 8-10:4-6:3-5:2-4 Homogeneous phase mixing in mass ratio, add its water of quality 3-7 times, room temperature 200-300W, 35-40KHz condition supersound extraction 10-15min, then at electric field intensity 20-40kV/cm, burst length 400-500 μ s, carries out high-pressure pulse electric extraction when pulse frequency 200-300Hz；It is 4.5-6.5 with breast acid for adjusting pH value, adds the enzyme of mixture quality 0.1-0.3%, in 45-55 DEG C of enzymolysis 20-48min；Enzymolysis solution concentrating under reduced pressure, lyophilization, low-temperature grinding to particle diameter are that namely 0.1-0.3mm obtains modified dietary fiber；
Described enzyme is xylanase, cellulase, laccase, pectase, tannase 6-10:3-5:2-4:2-4:1-3 Homogeneous phase mixing in mass ratio.
null7. can improve the Radix Glycyrrhizae plant beverage of immunity as claimed in claim 1，It is characterized in that，The preparation method of described bee pollen of maize extract，Comprise the steps: that 40-60:3-5:2-4 weighs bee pollen of maize respectively in mass ratio、Glutinous Semen Maydis must with defatted corn powder，First by bee pollen of maize、Glutinous Semen Maydis must be put in the ultrasonic washing unit equipped with 0.3-0.5% sodium bicarbonate solution respectively，In 200W under room temperature、40KHz cleans 3-5min，Drain，Then mix with defatted corn powder，Add the water of former bee pollen of maize quality 3-5 times，It is 4-6 with breast acid for adjusting pH value，In electric field intensity 20-40kV/cm，Burst length 400-500 μ s,High voltage pulse electric field processing is carried out when pulse frequency 200-300Hz，Then room temperature 400W、40KHz condition supersound process 10-15min；Being warming up to 40-50 DEG C, add the enzyme preparation of former bee pollen of maize quality 2-8%, stir, enzymolysis 15-20min, enzymolysis solution filters through 100-300 eye mesh screen, and namely filtrate obtain bee pollen of maize extract through ultrafiltration, concentrating under reduced pressure, lyophilization；
The quality group of described enzyme preparation becomes: protease: saccharifying enzyme: pectase: cellulase: lipase=11-13:5-10:4-6:3-5:1-3.
8. can improve the Radix Glycyrrhizae plant beverage of immunity as claimed in claim 1, it is characterized in that, the preparation method of described pectin decomposer, comprise the steps: fresh Fructus Crataegi marc, Pericarpium Vitis viniferae, pomace, megasse to be respectively put in the ultrasonic washing unit equipped with 1.2% sodium bicarbonate solution and clean 5-10min in 200W, 40KHz, drain, it is cut into phase equal-specification, length and width is 5-10mm, the sheet of thickness 3-5mm or fourth, 6-9:3-5:2-4:2-4 Homogeneous phase mixing in mass ratio, then in-21-25 DEG C of freezing 10-30min；Pulverizing immediately, particle diameter is 0.1-1mm；nullAdd the deionized water of ground product quality 3-5 times，Mix homogeneously，It is 2-3 with Fructus Citri Limoniae acid for adjusting pH value，At electric field intensity 35-45kV/cm under room temperature，Burst length 400-600 μ s,High voltage pulse electric field processing is carried out when pulse frequency 200-300Hz，Then normal pressure boils 30-50min，Bag filter，Filtering residue boils 40-60min with its filtrate normal pressure of quality 1-3 times,Bag filter，Merging filtrate，Kieselguhr filters，Filtrate is made to clarify，Being evaporated to solid content at 75 DEG C is 6-10%，It is rapidly cooled to room temperature，Add 95% ethanol that pH value is 2-3 of 1.5 times of volumes of concentrated solution，Stand 20-30min,Pectin is made to be precipitated out，4000r/min is centrifuged 5-10min，Reclaim precipitation，With the dehydrated alcohol eluting of 2 times of volumes，Centrifugal，Reclaim precipitation，So operation 2 times，What obtain is deposited in 40-60 DEG C of vacuum drying 2-3h,Obtain pectin，Add 40-50 DEG C of water of pectin quality 4-5 times，Stirring，Homogeneous phase mixing，Insulation，It is 4.5-5.5 with breast acid for adjusting pH value，Add the pectinase enzymatic hydrolysis 2-4h of 0.3-0.8U/mL,Enzymolysis solution filters，Filtrate is enzyme denaturing 10-15min in boiling water，It is cooled to room temperature，Being evaporated to solid content is more than 20%，Obtain pectin decomposer.
9. the preparation method that can improve the Radix Glycyrrhizae plant beverage of immunity as claimed in claim 1, it is characterised in that comprise the steps:
1) Radix Glycyrrhizae concentrated juice, fungi extracts, concentrated medlar juice, modified dietary fiber, bee pollen of maize extract, pectin decomposer, white sugar, Fructus Momordicae, Mel and citric acid are accurately weighed by formula；
2) first by Radix Glycyrrhizae concentrated juice and concentrated medlar juice Homogeneous phase mixing in uncovered jacketed pan, simultaneously, Fructus Momordicae is crushed to particle diameter 1-3mm and joins jacketed pan, jacketed pan steam pressure is 0.1-0.3MPa, mixes 10-30min, speed of agitator 100-300r/min while stirring, stand 5-10min, filter, obtain mixed concentrated liquid, standby；
3) in blend tank, add the pure water of White Sugar Quality 16-19 times, control temperature 80-90 DEG C, it is sequentially added into white sugar while stirring, Mel and citric acid, speed of agitator 80-120r/min, fully dissolve 20-30min, it is cooled to 75-85 DEG C, insulation, it is sequentially added into mixed concentrated liquid while stirring, fungi extracts, pectin decomposer, modified dietary fiber and bee pollen of maize extract, fully dissolve 20-30min, then through 200-400 order pipeline screen filtration, two sections of plate type heat exchangers are cooled to 1-4 DEG C, pump into sterilizing tapered shaped can, insulation, stand 20-40min, through 0.1-0.3 μm of aseptic filtration, obtain Radix Glycyrrhizae plant beverage semi-finished product；
4) step 3) semi-finished product that obtain are sterilized, namely cooling, sterile filling obtain the Radix Glycyrrhizae plant beverage that can improve immunity.
10. the preparation method that can improve the Radix Glycyrrhizae plant beverage of immunity as claimed in claim 9, it is characterised in that comprise the steps:
1) in parts by weight, Radix Glycyrrhizae concentrated juice 12 parts, fungi extracts 20 parts, concentrated medlar juice 10 parts are accurately weighed, modified dietary fiber 10 parts, bee pollen of maize extract 20 parts, pectin decomposer 5 parts, white sugar 150 parts, Fructus Momordicae 13 parts, Mel 9 parts, citric acid 2 parts；
2) first by Radix Glycyrrhizae concentrated juice and concentrated medlar juice Homogeneous phase mixing in uncovered jacketed pan, simultaneously, Fructus Momordicae is crushed to particle diameter 2mm and joins jacketed pan, jacketed pan steam pressure is 0.2MPa, mixes 20min, speed of agitator 200r/min while stirring, stand 8min, filter, obtain mixed concentrated liquid, standby；
3) in blend tank, add the pure water of White Sugar Quality 18 times, control temperature 85 DEG C, it is sequentially added into white sugar while stirring, Mel and citric acid, speed of agitator 100r/min, fully dissolve 25min, it is cooled to 80 DEG C, insulation, it is sequentially added into mixed concentrated liquid while stirring, fungi extracts, pectin decomposer, modified dietary fiber and bee pollen of maize extract, fully dissolve 25min, then through 300 order pipeline screen filtrations, two sections of plate type heat exchangers are cooled to 2 DEG C, pump into sterilizing tapered shaped can, insulation, stand 30min, through 0.2 μm of aseptic filtration, obtain Radix Glycyrrhizae plant beverage semi-finished product；
4) step 3) semi-finished product that obtain are sterilized, namely cooling, sterile filling obtain the Radix Glycyrrhizae plant beverage that can improve immunity.
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| CN105309979A (en) * | 2015-11-16 | 2016-02-10 | 浙江百兴食品有限公司 | Edible fungi functional beverage and preparation method thereof |
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| CN107156578A (en) * | 2017-06-15 | 2017-09-15 | 蔡敬侠 | A kind of herbal beverage for improving body immunity and preparation method thereof |
| CN108576504A (en) * | 2018-03-29 | 2018-09-28 | 广西壮族自治区农业科学院农产品加工研究所 | A kind of wax gourd high microsteping beverage |
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