CN105028260B - A kind of selection of shellfish Meat Quality new lines - Google Patents
A kind of selection of shellfish Meat Quality new lines Download PDFInfo
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- CN105028260B CN105028260B CN201510317799.1A CN201510317799A CN105028260B CN 105028260 B CN105028260 B CN 105028260B CN 201510317799 A CN201510317799 A CN 201510317799A CN 105028260 B CN105028260 B CN 105028260B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a kind of selection of shellfish Meat Quality new lines, the full sibs and/or family half sibs of more than 20 are initially set up, and each family is formed in different waters；Secondly Meat Quality index determining and apparent index measurement are carried out to each family formed；The genetic force of Meat Quality index is filtered out again more than 0.2, and filters out index of the absolute value of genetic correlation between Meat Quality index and apparent index more than 0.5；Then stay rate to filter out candidate's parent's family according to 20% sieve, optimal individual is selected as breeding parent in candidate's parent's family according to Apparent character index；Finally according to above selection, carry out by for seed selection, ultimately form the high new lines of Meat Quality index.The present invention is not on the premise of research object is dissected, by indirect breeding, with the method for selecting to be combined in families selecting and family, from can apparent index seed selection Meat Quality index measured directly.
Description
Technical field
The present invention relates to a kind of selection of biological new lines, specifically, it is related to a kind of shellfish Meat Quality new product
The selection of system.
Background technology
Shellfish is economic species important in China's sea-farming and pillar industry, with less investment, instant effect, economy effect
It is beneficial high, the advantages of ecological effect is good.But current shellfish culture lacks breeding, relies primarily on wild seedling or wild（Introduce）Parent
Bred, offspring's Meat Quality is poor, individual difference is big, resistance is weak, and disease and problem of death are increasingly severe, cultivate efficiency
Lowly.Therefore, the degeneration-resistant shellfish culture new varieties of good quality and high output are cultivated（System）, culture benefit is improved, with extremely important meaning
Justice.
With the continuous improvement of living standard, people more focus on local flavor and the nutrition of meat for the consumption of shellfish.No
Same local flavor and nutritional quality affects the consumption propensity of consumer, and then decides its commodity value.Meat Quality is to determine
The important economical trait of shellfish mouthfeel and nutritional quality, the new varieties that seed selection mouthfeel is good, nutrition is good are that the urgent of high-end market is essential
Ask.
The Meat Quality of shellfish be one kind can not live body character measured directly, thus, it is different to the seed selection of Meat Quality
In such as growth traits etc can direct measurement character.Most of Meat Qualities belong to quantitative character（quantitative
characters）, the character is controlled by multiple genes, and is to be mutually related between character, can be shown not in Breeding Process
With the collaborative variation of degree.The basic reason of collaborative variation is probably the pleiotropism of gene linkage and gene between character, it is also possible to
It is that each character is influenceed by same environmental factor.
The content of the invention
The purpose of the present invention is exactly, by the estimation to shellfish Meat Quality genetic parameter, to formulate optimal breeding system,
Breeding value is inferred by phenotypic number, the Breeding program chosen seeds to Meat Quality genotype is realized.
A kind of selection of shellfish Meat Quality new lines, its operating procedure is：
（1）Using the high one or more colonies of Genetic variability as seed selection underlying group, by nido experimental design or
The full sibs and/or family half sibs of more than 20 are set up in factorial experiment design, and each family is formed in different waters；
（2）Carry out Meat Quality index determining to each family for forming, Meat Quality index includes total protein content, total
One in fat content, glycogen content, delicious amino acid content, unsaturated fatty acid content, Zn-ef ficiency content, selenium element content
Plant or a variety of；Apparent index measurement is carried out to each family formed simultaneously, apparent index includes can live body shell measured directly
One or more in height, shell length, wide shell, weight in wet base, closed shell flesh pulling force；
（3）Genetic parameters estimate is carried out to the Meat Quality index of each family and apparent index formed, meat is filtered out
The genetic force of matter characteristic index filters out the absolute of genetic correlation between Meat Quality index and apparent index more than 0.2
It is worth the index more than 0.5；
（4）By calculating the family breeding value of Meat Quality index, according to family breeding value ranking height, according to 20%
Sieve stays rate to filter out candidate's parent's family；Pass through the genetic correlation between the Meat Quality index and Apparent character index of acquisition, root
Select optimal individual as breeding parent in candidate's parent's family according to Apparent character index, realize in families selecting and family
The system of selection being combined of selection.
（5）According to above selection, carry out by for seed selection, ultimately form the high new lines of Meat Quality index.
Further, described genetic parameters estimate is to carry out statistical analysis with Quantitative Genetics statistical analysis software.
Further, described genetic parameter includes genetic force, genetic correlation and family breeding value.
Compared with prior art, the present invention can be on the premise of research object not be dissected, by indirect breeding, with family
Selection with the system of selection being combined that selects in family, from can apparent index seed selection Meat Quality index measured directly,
Had broad application prospects in terms of the seed selection of shellfish Meat Quality.
Embodiment
Embodiment：
A kind of selection of long oyster Meat Quality new lines, its operating procedure is：
（1）Regular female, male long each 9 of the oyster individual of hull shape is selected as the close shellfish of breeding, is set using partial factors experiment
Meter, carries out single pair of single cross with insemination, sets up 25 family full-sibses, each family is marked respectively, take strict quarantine measures to carry out
The young is cultivated.Each family is respectively placed in Yantai Kongdong Island, Weihai Rushan and Dongying Huanghe River Delta Offshore Area and carried out by forming stage
Form.
（2）Determine the true of glycogen content and total protein content in long 25 family full-sibs Meat Quality indexs of oyster
Value, while measuring shell length and the wide two apparent indexs of shell；
（3）Pass through genetic parameters estimate software ASReml（3.0, UK）The glycogen of each family of 3 aquaculture sea areas is contained
Amount and two Meat Quality indexs of total protein content carry out genetic force and genetic correlation point with shell length and the apparent index of wide two of shell
Analysis.The genetic force for obtaining Meat Quality index glycogen content is 0.30 ± 0.10, the genetic force of total protein content for 0.26 ±
0.10.It is 0.78 ± 0.14 to obtain Kongdong Island marine site Meat Quality index glycogen content and the wide genetic correlation of apparent index shell,
The genetic correlation of total protein content and shell length is -0.89 ± 0.08.
（4）With genetic parameters estimate software ASReml（3.0, UK）Calculate the long oyster meat characteristic index sugar in each marine site
The family breeding value of former content and total protein content, according to each marine site family breeding value ranking height, stays rate to sieve according to 20% sieve
Select candidate parent family 5.According to glycogen content and the wide genetic correlation of shell, carry out favorable selection wide to shell chooses glycogen
The high individual of content is used as breeding parent；According to the genetic correlation of total protein content and shell length, negative itemsets, choosing are carried out to shell length
The high individual of total protein content is taken as breeding parent.
（5）According to above selection, carry out by for seed selection, ultimately forming that glycogen content is high and the high length of total protein content
Oyster new lines.
The present invention is not limited to above-mentioned preferred forms, and anyone should learn that the knot made under the enlightenment of the present invention
Structure change, it is every with it is of the invention have it is same or similar as technical scheme, belong to protection scope of the present invention.
Claims (3)
1. a kind of selection of shellfish Meat Quality new lines, its operating procedure is：
（1）Using the high one or more colonies of Genetic variability as seed selection underlying group, 20 are set up by factorial experiment design
Full sibs and/or family half sibs above, marks each family respectively, takes strict quarantine measures to carry out young culture, forms
Stage is formed each family in different waters；
（2）Meat Quality index determining is carried out to each family formed, Meat Quality index includes total protein content, total fat
Content, glycogen content, delicious amino acid content, unsaturated fatty acid content, Zn-ef ficiency content, one kind in selenium element content or
It is a variety of；Apparent index measurement is carried out to each family formed simultaneously, apparent index includes can live body shell height measured directly, shell
One or more in length, wide shell, weight in wet base, closed shell flesh pulling force；
（3）Genetic parameters estimate is carried out to the Meat Quality index of each family and apparent index formed, succulence is filtered out
The genetic force of shape index filters out the absolute value of the genetic correlation between Meat Quality index and apparent index and existed more than 0.2
More than 0.5 index；
（4）By calculating the family breeding value of Meat Quality index, according to family breeding value ranking height, stayed according to 20% sieve
Rate filters out candidate's parent's family；By the genetic correlation between the Meat Quality index and Apparent character index of acquisition, according to table
See characteristic index and optimal individual is selected in candidate's parent's family as breeding parent, realize families selecting with being selected in family
The system of selection being combined；
（5）According to above selection, carry out by for seed selection, ultimately form the high new lines of Meat Quality index.
2. a kind of selection of shellfish Meat Quality new lines according to claim 1, it is characterised in that described something lost
It is to carry out statistical analysis with Quantitative Genetics statistical analysis software to pass Parameter analysis.
3. a kind of selection of shellfish Meat Quality new lines according to claim 2, it is characterised in that described something lost
Passing parameter includes genetic force, genetic correlation and family breeding value.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510317799.1A CN105028260B (en) | 2015-06-11 | 2015-06-11 | A kind of selection of shellfish Meat Quality new lines |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510317799.1A CN105028260B (en) | 2015-06-11 | 2015-06-11 | A kind of selection of shellfish Meat Quality new lines |
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| Publication Number | Publication Date |
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| CN105028260A CN105028260A (en) | 2015-11-11 |
| CN105028260B true CN105028260B (en) | 2017-10-31 |
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| CN201510317799.1A Expired - Fee Related CN105028260B (en) | 2015-06-11 | 2015-06-11 | A kind of selection of shellfish Meat Quality new lines |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577415B (en) * | 2016-12-26 | 2019-04-02 | 中国海洋大学 | A kind of producing method for seed of the long oyster new lines of black shell |
| CN106614164B (en) * | 2016-12-26 | 2019-04-30 | 中国海洋大学 | A kind of breeding method of the long oyster new lines of pure white shell |
| CN107593598A (en) * | 2017-10-10 | 2018-01-19 | 广西金诚双丰农牧科技有限公司 | Reduce the breeding method of bamboo rat stable breeding stress reaction |
| CN112568157B (en) * | 2020-12-04 | 2022-11-25 | 浙江省海洋水产养殖研究所 | Breeding method of tegillarca granosa strain with low cadmium enrichment |
| CN113331096B (en) * | 2021-06-07 | 2022-06-03 | 湛江华南贝类研究院 | Settleable crassostrea gigas population breeding method |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2007133075A2 (en) * | 2006-05-12 | 2007-11-22 | Institute For Pig Genetics B.V. | Igf2 marker assisted selection for porcine reproductive traits |
| WO2008024227A2 (en) * | 2006-08-22 | 2008-02-28 | Monsanto Technology Llc | Genetic markers and methods for improving swine genetics |
| CN102405883A (en) * | 2011-09-16 | 2012-04-11 | 厦门大学 | Method for building pedigree of marsupenaeus japonicus and breeding |
| CN102550485B (en) * | 2012-03-06 | 2013-09-25 | 天津奥群牧业有限公司 | Method for breeding improved mutton sheep strain |
| CN103250666B (en) * | 2013-05-14 | 2014-08-20 | 中国水产科学研究院黄海水产研究所 | Method for breeding blue crab families |
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