CN105028260A - Breeding method for shellfish meat trait new strain - Google Patents
Breeding method for shellfish meat trait new strain Download PDFInfo
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- CN105028260A CN105028260A CN201510317799.1A CN201510317799A CN105028260A CN 105028260 A CN105028260 A CN 105028260A CN 201510317799 A CN201510317799 A CN 201510317799A CN 105028260 A CN105028260 A CN 105028260A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a breeding method for a shellfish meat trait new strain. The breeding method comprises the following steps: firstly constructing more than 20 full-sib and/or half-sib families, and breeding all the families in different sea areas; performing meat trait index measurement and apparent index measurement on all the bred families; then screening out meat trait indexes with the heritability larger than 0.2, and screening out indexes with the genetic correlation absolute value larger than 0.5 between the meat trait indexes and the appearance indexes; screening out candidate parent families according to the retention rate of 20 percent, and selecting optimal individuals from the candidate parent families to be used as reproduction parents according to the appearance trait indexes; finally carrying out generational breeding according to the breeding method, and finally forming the new strain with high meat trait index. According to the breeding method, on the premise that a research object is not dissected, a method of combining family selection and within-family selection is adopted for breeding the meat trait index from the directly measurable appearance indexes by indirect breeding.
Description
Technical field
The present invention relates to a kind of selection of biological new lines, specifically, relate to a kind of selection of shellfish Meat Quality new lines.
Background technology
The advantages such as shellfish is economic species important in China's mariculture and pillar industry, has less investment, instant effect, high financial profit, and ecological effect is good.But shellfish culture lacks breeding at present, main wild seedling or wild (introduction) parent of relying on is bred, and offspring's Meat Quality is poor, individual difference is large, resistance is weak, and disease and problem of death are more and more serious, cultivation inefficiency.Therefore, cultivate the shellfish culture new varieties (being) that good quality and high output is degeneration-resistant, improve culture benefit, be extremely important.
Along with improving constantly of living standard, people, for the consumption of shellfish, focus on local flavor and the nutrition of meat more.Different local flavors and nutritional quality affect the consumption propensity of consumer, and then decide its commodity value.Meat Quality is the important economical trait determining shellfish mouthfeel and nutritional quality, and the new varieties that seed selection mouthfeel is good, nutrition is good are active demands of high-end market.
The Meat Quality of shellfish be a kind of can not the proterties directly measured of live body, thus, to the seed selection of Meat Quality be different from such as growth traits and so on can direct Metric traits.Most of Meat Quality belongs to quantitative character (quantitativecharacters), and this proterties is controlled by multiple gene, and be mutually related between proterties, can demonstrate collaborative variation in various degree in Breeding Process.Between proterties, the basic reason of collaborative variation may be the pleiotropy of gene linkage and gene, also may be the impact that each proterties is subject to same environmental factor.
Summary of the invention
Object of the present invention is exactly by the estimation to shellfish Meat Quality genetic parameter, formulates best breeding system, infers breeding value by phenotypic value, realizes the Breeding program to the seed selection of Meat Quality genotype.
A selection for shellfish Meat Quality new lines, its operating procedure is:
(1) using the high one or more colonies of Genetic variability as seed selection underlying group, set up full sibs and/or the family half sibs of more than 20 by nido experimental design or factorial experiment design, and each family is formed in different waters;
(2) Meat Quality index determining is carried out to each family formed, Meat Quality index comprise in total protein content, total lipid content, glycogen content, delicious amino acid content, unsaturated fatty acid content, Zn-ef ficiency content, selenium constituent content one or more; Apparent index measurement is carried out to each family formed simultaneously, apparent index comprise can the shell directly measured of live body high, shell is long, shell is wide, one or more in weight in wet base, closed shell flesh pulling force;
(3) genetic parameters estimate is carried out to the Meat Quality index of each family formed and apparent index, filter out the heritability of Meat Quality index more than 0.2, and filter out the index of absolute value more than 0.5 of the genetic correlation between Meat Quality index and apparent index;
(4) by calculating the family breeding value of Meat Quality index, according to family breeding value rank height, the sieve according to 20% stays rate to filter out candidate parent family; By the genetic correlation between the Meat Quality index that obtains and Apparent character index, in candidate parent family, select best individuality as breeding parent according to Apparent character index, realize the families selecting system of selection that combine selected interior with family.
(5) according to above selection, carry out, by for seed selection, finally forming the high new lines of Meat Quality index.
Further, described genetic parameters estimate uses Quantitative Genetics statistical analysis software to carry out statistical analysis.
Further, described genetic parameter comprises heritability, genetic correlation and family breeding value.
Compared with prior art, the present invention can under the prerequisite of not dissecting research object, pass through indirect breeding, use families selecting and the system of selection combined selected in family, from the apparent index seed selection Meat Quality index that can directly measure, have broad application prospects in the seed selection of shellfish Meat Quality.
Embodiment
Embodiment:
A selection for long oyster Meat Quality new lines, its operating procedure is:
(1) select individual each 9 the close shellfishes of conduct breeding of regular female, the male long oyster of hull shape, adopt partial factors experimental design, carry out list and insemination is joined to single cross, set up 25 family full-sibses, mark each family respectively, take strict quarantine measures to carry out young cultivation.Each family is placed in Kongdong Island, Yantai, Rushan, Weihai and Dongying Huanghe River Delta Offshore Area and forms by forming stage respectively.
(2) measure the actual value of glycogen content and total protein content in long oyster 25 family full-sibs Meat Quality indexs, measure shell length and wide two the apparent indexs of shell simultaneously;
(3) by genetic parameters estimate software ASReml(3.0, UK) to glycogen content and total protein content two Meat Quality indexs of each family of 3 aquaculture sea area and shell is long and wide two the apparent indexs of shell carry out heritability and Correlation Analysis.The heritability obtaining Meat Quality index glycogen content is 0.30 ± 0.10, and the heritability of total protein content is 0.26 ± 0.10.Obtain marine site, Kongdong Island Meat Quality index glycogen content and the wide genetic correlation of apparent index shell is 0.78 ± 0.14, total protein content and the long genetic correlation of shell are-0.89 ± 0.08.
(4) genetic parameters estimate software ASReml(3.0 is used, UK) the family breeding value of each marine site long oyster meat characteristic index glycogen content and total protein content is calculated, according to each marine site family breeding value rank height, the sieve according to 20% stays rate to filter out candidate parent family 5.According to glycogen content and the wide genetic correlation of shell, carry out favorable selection to shell is wide, choose the high individuality of glycogen content as breeding parent; According to the genetic correlation that total protein content and shell are grown, to shell progress row negative itemsets, choose the high individuality of total protein content as breeding parent.
(5) according to above selection, carry out, by for seed selection, finally forming the long oyster new lines that glycogen content is high and total protein content is high.
The present invention is not limited to above-mentioned preferred forms, and anyone should learn the structure change made under enlightenment of the present invention, and every have identical or akin technical scheme with the present invention, all belongs to protection scope of the present invention.
Claims (3)
1. a selection for shellfish Meat Quality new lines, its operating procedure is:
(1) using the high one or more colonies of Genetic variability as seed selection underlying group, set up full sibs and/or the family half sibs of more than 20 by nido experimental design or factorial experiment design, and each family is formed in different waters;
(2) Meat Quality index determining is carried out to each family formed, Meat Quality index comprise in total protein content, total lipid content, glycogen content, delicious amino acid content, unsaturated fatty acid content, Zn-ef ficiency content, selenium constituent content one or more; Apparent index measurement is carried out to each family formed simultaneously, apparent index comprise can the shell directly measured of live body high, shell is long, shell is wide, one or more in weight in wet base, closed shell flesh pulling force;
(3) genetic parameters estimate is carried out to the Meat Quality index of each family formed and apparent index, filter out the heritability of Meat Quality index more than 0.2, and filter out the index of absolute value more than 0.5 of the genetic correlation between Meat Quality index and apparent index;
(4) by calculating the family breeding value of Meat Quality index, according to family breeding value rank height, the sieve according to 20% stays rate to filter out candidate parent family; By the genetic correlation between the Meat Quality index that obtains and Apparent character index, in candidate parent family, select best individuality as breeding parent according to Apparent character index, realize the families selecting system of selection that combine selected interior with family;
(5) according to above selection, carry out, by for seed selection, finally forming the high new lines of Meat Quality index.
2. the selection of a kind of shellfish Meat Quality new lines according to claim 1, is characterized in that described genetic parameters estimate uses Quantitative Genetics statistical analysis software to carry out statistical analysis.
3. the selection of a kind of shellfish Meat Quality new lines according to claim 2, is characterized in that described genetic parameter comprises heritability, genetic correlation and family breeding value.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577415A (en) * | 2016-12-26 | 2017-04-26 | 中国海洋大学 | Seed production method of black-shell pacific oyster new strain |
| CN106614164A (en) * | 2016-12-26 | 2017-05-10 | 中国海洋大学 | Cultivation method for new strain of pure white shell pacific oyster |
| CN107593598A (en) * | 2017-10-10 | 2018-01-19 | 广西金诚双丰农牧科技有限公司 | Reduce the breeding method of bamboo rat stable breeding stress reaction |
| CN112568157A (en) * | 2020-12-04 | 2021-03-30 | 浙江省海洋水产养殖研究所 | Breeding method of tegillarca granosa strain with low cadmium enrichment |
| CN113331096A (en) * | 2021-06-07 | 2021-09-03 | 湛江华南贝类研究院 | Settleable crassostrea gigas population breeding method |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106577415A (en) * | 2016-12-26 | 2017-04-26 | 中国海洋大学 | Seed production method of black-shell pacific oyster new strain |
| CN106614164A (en) * | 2016-12-26 | 2017-05-10 | 中国海洋大学 | Cultivation method for new strain of pure white shell pacific oyster |
| CN106577415B (en) * | 2016-12-26 | 2019-04-02 | 中国海洋大学 | A kind of producing method for seed of the long oyster new lines of black shell |
| CN106614164B (en) * | 2016-12-26 | 2019-04-30 | 中国海洋大学 | A kind of breeding method of the long oyster new lines of pure white shell |
| CN107593598A (en) * | 2017-10-10 | 2018-01-19 | 广西金诚双丰农牧科技有限公司 | Reduce the breeding method of bamboo rat stable breeding stress reaction |
| CN112568157A (en) * | 2020-12-04 | 2021-03-30 | 浙江省海洋水产养殖研究所 | Breeding method of tegillarca granosa strain with low cadmium enrichment |
| CN113331096A (en) * | 2021-06-07 | 2021-09-03 | 湛江华南贝类研究院 | Settleable crassostrea gigas population breeding method |
| CN113331096B (en) * | 2021-06-07 | 2022-06-03 | 湛江华南贝类研究院 | Settleable crassostrea gigas population breeding method |
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