CN105018610A - 莫氏立克次体及恙虫病东方体双重实时荧光定量pcr检测体系及检测方法 - Google Patents
莫氏立克次体及恙虫病东方体双重实时荧光定量pcr检测体系及检测方法 Download PDFInfo
- Publication number
- CN105018610A CN105018610A CN201510408885.3A CN201510408885A CN105018610A CN 105018610 A CN105018610 A CN 105018610A CN 201510408885 A CN201510408885 A CN 201510408885A CN 105018610 A CN105018610 A CN 105018610A
- Authority
- CN
- China
- Prior art keywords
- probe
- pmd18
- orientia tsutsugamushi
- rickettsia
- quantitative pcr
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (7)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510408885.3A CN105018610B (zh) | 2015-07-14 | 2015-07-14 | 莫氏立克次体及恙虫病东方体双重实时荧光定量pcr检测体系及检测方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510408885.3A CN105018610B (zh) | 2015-07-14 | 2015-07-14 | 莫氏立克次体及恙虫病东方体双重实时荧光定量pcr检测体系及检测方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105018610A true CN105018610A (zh) | 2015-11-04 |
CN105018610B CN105018610B (zh) | 2019-02-15 |
Family
ID=54408908
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510408885.3A Expired - Fee Related CN105018610B (zh) | 2015-07-14 | 2015-07-14 | 莫氏立克次体及恙虫病东方体双重实时荧光定量pcr检测体系及检测方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105018610B (zh) |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104962628A (zh) * | 2015-06-25 | 2015-10-07 | 中华人民共和国北京出入境检验检疫局 | 一种用于同时检测斑点热群立克次体和莫氏立克次体的检测试剂盒及引物和探针 |
CN113265479A (zh) * | 2021-05-26 | 2021-08-17 | 中国人民解放军军事科学院军事医学研究院 | 检测莫氏立克次体的引物组合物及其应用 |
CN114836579A (zh) * | 2022-06-02 | 2022-08-02 | 昆明理工大学 | 中枢神经系统感染性病原体的多重荧光定量pcr检测引物组合 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104651498A (zh) * | 2015-01-23 | 2015-05-27 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | 立克次体甄别检测基因芯片的制备和用途 |
-
2015
- 2015-07-14 CN CN201510408885.3A patent/CN105018610B/zh not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104651498A (zh) * | 2015-01-23 | 2015-05-27 | 中国人民解放军军事医学科学院放射与辐射医学研究所 | 立克次体甄别检测基因芯片的制备和用途 |
Non-Patent Citations (2)
Title |
---|
付秀萍等: "TaqMan-MGB 探针实时荧光定量PCR检测恙虫病东方体方法的建立", 《中国媒介生物学及控制杂志》 * |
杨晓等: "实时荧光定量PCR检测莫氏立克次体", 《解放军医学杂志》 * |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104962628A (zh) * | 2015-06-25 | 2015-10-07 | 中华人民共和国北京出入境检验检疫局 | 一种用于同时检测斑点热群立克次体和莫氏立克次体的检测试剂盒及引物和探针 |
CN113265479A (zh) * | 2021-05-26 | 2021-08-17 | 中国人民解放军军事科学院军事医学研究院 | 检测莫氏立克次体的引物组合物及其应用 |
CN114836579A (zh) * | 2022-06-02 | 2022-08-02 | 昆明理工大学 | 中枢神经系统感染性病原体的多重荧光定量pcr检测引物组合 |
CN114836579B (zh) * | 2022-06-02 | 2024-05-07 | 昆明理工大学 | 中枢神经系统感染性病原体的多重荧光定量pcr检测引物组合 |
Also Published As
Publication number | Publication date |
---|---|
CN105018610B (zh) | 2019-02-15 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106947838B (zh) | 非洲猪瘟病毒非结构基因实时荧光lamp检测引物组、试剂盒及检测方法 | |
Chen et al. | Rapid Sanger sequencing of the 16S rRNA gene for identification of some common pathogens | |
WO2016188144A1 (zh) | 一种具有增强鉴别能力的str基因座荧光标记复合扩增试剂盒及其应用 | |
Hou et al. | Droplet-based digital PCR (ddPCR) and its applications | |
Mochizuki et al. | Multiplex PCR and Genescan analysis to detect immunoglobulin heavy chain gene rearrangement in feline B-cell neoplasms | |
US20150322515A1 (en) | Methods and compositions for detecting target snp | |
CN101624629B (zh) | 单管中多重靶核酸的pcr检测方法及其试剂盒 | |
CN102146466A (zh) | 检测布鲁氏菌的试剂及复合探针荧光定量pcr检测布鲁氏菌的方法 | |
CN110564895A (zh) | 猫疱疹病毒fhv-1的raa恒温荧光检测引物探针组、试剂盒及方法 | |
CN111926116A (zh) | 快速定量检测鸭腺病毒4型的引物和探针及其检测方法与应用 | |
CN105018610A (zh) | 莫氏立克次体及恙虫病东方体双重实时荧光定量pcr检测体系及检测方法 | |
US9994922B2 (en) | Methods and compositions for assessing copy number of target polynecleotides | |
CN102154487A (zh) | 检测土拉弗朗西斯菌的试剂及复合探针荧光定量pcr检测土拉弗朗西斯菌的方法 | |
US20130122484A1 (en) | Diagnostic method for determining animals persistently infected (pi) with bovine viral diarrhea virus (bvdv) | |
CN103472236A (zh) | Dna结合蛋白的检测方法 | |
CN110257544B (zh) | 麦角病菌荧光定量pcr检测试剂及检测试剂盒和应用 | |
CN103740822B (zh) | 一种spf小鼠病原菌核酸荧光定量pcr联合快速检测试剂盒及检测方法 | |
CN113215325A (zh) | 二维pcr单管闭管检测多种hpv亚型的反应体系、方法及试剂盒 | |
KR101334072B1 (ko) | 핵산 정량 방법 및 키트 | |
JP6853523B2 (ja) | ヘリカーゼを用いたpcr | |
Wu et al. | Multiplex detection of five canine viral pathogens for dogs as laboratory animals by the Luminex xTAG assay | |
CN109234463A (zh) | 用于检测猪传染性胃肠炎病毒的引物及探针、荧光定量pcr试剂盒及方法和应用 | |
CN104975095A (zh) | 恙虫病东方体TaqMan-MGB探针实时荧光定量PCR检测体系统及其检测方法 | |
US20240068049A1 (en) | Assays, kits and methods for detection of bovine respiratory disease complex-associated pathogens | |
CN111424074B (zh) | 一种定量小鼠源组织痕量残留的核酸检测试剂盒 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information |
Inventor after: Gao Yufeng Inventor after: Wang Huiyu Inventor after: Yue Qiaoyun Inventor after: Wang Jing Inventor after: Song Fenglin Inventor after: Liang Jie Inventor after: Chen Chang Inventor after: Wan Xue Inventor after: Cheng Xiaolan Inventor after: Jiang Lu Inventor after: Liu Fang Inventor after: Han Xueqing Inventor before: Gao Yufeng Inventor before: Song Fenglin Inventor before: Wan Xue Inventor before: Cheng Xiaolan Inventor before: Jiang Lu |
|
COR | Change of bibliographic data | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190215 Termination date: 20190714 |