CN105010725A - Feed production method by adding enzyme producing inducer into edible fungus brans - Google Patents
Feed production method by adding enzyme producing inducer into edible fungus brans Download PDFInfo
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- CN105010725A CN105010725A CN201510385987.8A CN201510385987A CN105010725A CN 105010725 A CN105010725 A CN 105010725A CN 201510385987 A CN201510385987 A CN 201510385987A CN 105010725 A CN105010725 A CN 105010725A
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Abstract
The present invention discloses a feed production method by adding an enzyme producing inducer into edible fungus brans, which consists of the following steps: crushing fungus sticks to obtain fungus brans, adding an enzyme producing inducer, culturing under suitable conditions, and eluting the fermentation system to obtain ligninase and feed. Compared to other feed production technology using biomass, the feed production method can either obtain ligninase, increase the contents of active nutritional substances of fungus bran saccharides, organic acids, bioactive substances, etc. by promoting the edible fungus mycelium fermentation conversion processes and enzymolysis, increase the palatability of edible fungus residues, and reduce the generated pollution in the production process of edible fungi.
Description
Technical field
Add to edible fungus bran and produce the method that enzyme inducer produces feed, be specifically related to utilize edible fungus bran to produce the method for feed.Belong to technical field of bioengineering.
Background technology
Edible fungus bran to refer in Edible Fungi process after fruiting or the synthesis comprising edible mushroom and biomass material of not yet fruiting, is rich in mycoprotein, is therefore called as bacterium chaff albumen.According to surveying and determination, in bacterium chaff, protein content reaches 12% ~ 21%.But because edible mushroom chaff is rich in cellulose and lignin such as husk, straw, wood chip etc. grow out multiple, although bacterium chaff contains a large amount of bacterium chaff albumen, but it can not directly add in animal diet as feed, because the crude fibre of original edible fungi residues is very high, exceed the standard that conventional animal is digested and assimilated, and can not full feeding condition in smell and palatability, can't preserve for a long time.Therefore at present edible fungi residues is all carry out burning or landfill after being used to dry, and may cause the breeding of pathogen in the process of drying, and burns and also can cause air pollution.Not only do not play the economic worth of bacterium chaff fully, also can bring pollution on the contrary.
Summary of the invention:
A kind of interpolation to edible fungus bran is the object of the present invention is to provide to produce the method that enzyme inducer produces feed, the bacterium rod containing hypha of edible fungus is utilized to be raw material, add wherein and produce enzyme induction liquid promotion edible mushroom making full use of biomass material, and then obtain lignoenzyme and feed.The bacterium rod in agricultural production is made to obtain high-valued recycling.
Bacterium rod refers to cotton seed hulls, wood chip, straw, corncob, bagasse and multiple kinds of crops stalk, industrial waste for primary raw material, is used for the bar-shaped solid mixt producing fruit body of edible fungi or fructification of gathering is later remaining after inoculation hypha of edible fungus cultivates a period of time.When hypha of edible fungus grows in the medium, need to produce a large amount of lignoenzyme to decompose lignocellulosic in matrix to obtain nutrition.Therefore, results or not yet gather in the crops fructification bacterium rod in containing a large amount of hypha of edible fungus.The concrete composition of bacterium rod can not as the restriction to feed producing method of the present invention.
Industrial waste is mainly selected from vinegar grain, vinasse, paper mill effluent.
Edible mushroom is the artificial mushroom cultivated or can be under wild environment biology and eat.Lignoenzyme can be secreted to decompose lignocellulosic in matrix to obtain nutrition in its growth course.Described edible mushroom is mainly selected from mushroom (Agaricus campestris), mushroom (Lentinula edodes), agrocybe (Agrocybe aegerita), Hericium erinaceus (Hericium erinaceum), phoenix-tail mushroom (Pleurotus sajor-caju), agaricus bisporus (Agaricusbisporus), auricularia auriculajudae (Auricularia auricula), Cordyceps sinensis (Cordyceps sinensis), glossy ganoderma (Ganoderma lucidum), pycnoporus samguineus (Pycnoporus sanguineus), gold ear (Tremellaaurantialba), grey mushroom (abalonelike) (Pleurotus abalonus), flat mushroom (Pleurotus ostreatus), asparagus (Flammulina velutipes), pleurotus eryngii (Pleurotus eryngii), elegant precious mushroom (Pleurotus geesteranus), coprinus comatus (Coprinus comatus), Pleurotus nebrodensis (Pleurotus nebrodensis), lung shape picks up the ears (Pleurotuspulmonarius), Agricus blazei (Agaricus blazei) etc.
Technical scheme of the present invention:
The invention provides a kind of method utilizing edible fungus stick to produce feed, specifically comprise the steps: that the break process of bacterium rod obtains bacterium chaff; Add and produce enzyme induction liquid; Cultivate under suitable condition; Fermentation system wash-out obtains lignoenzyme and feed.
1. broken bacterium rod obtains bacterium chaff
After the bacterium of fructification of or not yet gathering rod removing polybag, utilize pulverizer that the fragmentation of bacterium rod is obtained uniform bacterium chaff.
2. add induction and produce enzyme liquid
The inducer that enzyme liquid contains lignoenzyme is produced in induction used, and other substances provide nitrogen element and trace element for hypha of edible fungus, can also adjust carbon-nitrogen ratio (C/N) and the pH value of system in addition, stimulates mycelia diauxic growth.Induction is produced enzyme liquid and is obtained by the prolonged and repeated test in this laboratory.
A kind of concrete embodiment, the usual addition that enzyme liquid is produced in every 10kg bacterium chaff induction is 10-50L, 10-30L can be selected further, the diauxic growth that more effectively can stimulate hypha of edible fungus in bacterium chaff when 12,13,15,16,17,19,20,23,25 or 28L is selected, the high expression of induction lignoenzyme and secretion when concrete.
3. cultivate under suitable condition
Suitable extraneous condition of culture can change the physiological status of hypha of edible fungus, better expression and secretion lignoenzyme, and then biomass material is fully degraded.
A concrete embodiment, cultivation temperature is generally 17-36 DEG C, can be chosen as 22-30 DEG C further, or is specifically chosen as 22,24,25,27,28,30 DEG C.The time of cultivating under above temperature conditions is 24-168 hour, or selects 24,72,96,120 or 144 hours further, can obtain that lignoenzyme content is higher, lignocellulosic utilizes bacterium chaff more thoroughly.
4. the wash-out of fermentation system
What obtain to step 3 contains the distilled water immersion adding 1-20 times of volume in the fermentation maturation system of a large amount of lignoenzyme, and vibration is filtered.Can select 1-10 further, the lignoenzyme that mycelia can be made when being specifically chosen as 1,3,5,7,9 times to secrete is dissolved in solution as much as possible and does not hurt mycelia again.The lignoenzyme total amount obtained when distilled water volume is very few will be very few, when distilled water volume is excessive, the feed nutrition material obtained will be made to reduce.
Beneficial effect of the present invention:
The invention provides a kind of method utilizing edible fungus stick to produce feed.Be a kind of new way of bacterium chaff higher value application, in production process, can also lignoenzyme be obtained.Both can accomplish twice laid environmental protect, the production cost of lignoenzyme can be reduced again, there is good application prospect.It is a kind of brand-new feed production method.
Detailed description of the invention:
Below in conjunction with embodiment, the present invention is described further.Following examples are only several specific embodiment of the present invention, but design concept of the present invention is not limited thereto, and all changes utilizing this design the present invention to be carried out to unsubstantiality, all should belong to the behavior of invading scope.
Embodiment 1
Get 10kg flat mushroom bacterium rod, fragmentation adds the product enzyme induction liquid of 15L after obtaining bacterium chaff, cultivates 5 days at 28 DEG C, the system obtained adds 150L distilled water immersion, vibration, filters and obtains lignoenzyme crude enzyme liquid and bacterium chaff residue, for subsequent use after bacterium chaff residue is dried, obtain feed.
Embodiment 2
Get 10kg mushroom bacteria rod, fragmentation adds the product enzyme induction liquid of 20L after obtaining bacterium chaff, cultivates 4 days at 25 DEG C, the system obtained adds 200L distilled water immersion, vibration, filters and obtains lignoenzyme crude enzyme liquid and bacterium chaff residue, for subsequent use after bacterium chaff residue is dried, obtain feed.
Embodiment 3
Get 10kg pycnoporus samguineus bacterium rod, fragmentation adds the product enzyme induction liquid of 25L after obtaining bacterium chaff, cultivates 3 days at 22 DEG C, the system obtained adds 250L distilled water immersion, vibration, filters and obtains lignoenzyme crude enzyme liquid and bacterium chaff residue, for subsequent use after bacterium chaff residue is dried, obtain feed.
Claims (6)
1. one kind is added the method for producing enzyme inducer production feed to edible fungus bran, be characterized in that the bacterium chaff to obtain after edible fungus stick fragmentation is raw material, add product enzyme inducer, promote that hypha of edible fungus produces feed to living beings base-material trans-utilization, the method comprises the following steps:
A) by after the bacterium of fructification of or not yet gathering rod removing polybag, utilize pulverizer that the fragmentation of bacterium rod is obtained uniform bacterium chaff;
B) in obtained bacterium chaff, add product enzyme induction liquid according to the ratio of solid-liquid mass volume ratio 1: 1-4, after mixing, obtain fermentation system;
C) fermentation system is cultivated 24-168 hour under 17-36 DEG C of condition, carry out fermenting and producing lignocellulolyticenzymes;
The distilled water wash-out of the fermentation maturation system 1-10 times of volume d) obtained obtains crude enzyme liquid;
E) after wash-out, remaining solid matter is feed.
2. according to claim 1 interpolation to edible fungus bran produces the method that enzyme inducer produces feed, it is characterized in that described product enzyme induction formula of liquid is: in mass, urea 0.1 ~ 5%, ammonium nitrate 0.2 ~ 6%.
3. according to claim 1 interpolation to edible fungus bran produces the method that enzyme inducer produces feed, it is characterized in that every 10kg bacterium rod needs the product enzyme induction liquid of 10L-40L.
4. according to claim 1 interpolation to edible fungus bran produces the method that enzyme inducer produces feed, it is characterized in that cultivation temperature is 17-36 DEG C.
5. according to claim 1 interpolation to edible fungus bran produces the method that enzyme inducer produces feed, it is characterized in that incubation time is 24-168 hour.
6. according to claim 1 interpolation to edible fungus bran produces the method that enzyme inducer produces feed, it is characterized in that the mass volume ratio of fermentation system and each wash-out water is 1: 1-10.
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| CN201510385987.8A CN105010725A (en) | 2015-07-01 | 2015-07-01 | Feed production method by adding enzyme producing inducer into edible fungus brans |
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| CN201510385987.8A CN105010725A (en) | 2015-07-01 | 2015-07-01 | Feed production method by adding enzyme producing inducer into edible fungus brans |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812431A (en) * | 2009-02-23 | 2010-08-25 | 湖南省畜牧兽医研究所 | Method utilizing bacterisugar to prepare feed enzyme |
| CN102715339A (en) * | 2012-06-01 | 2012-10-10 | 陈华友 | Microorganism fodder production method based on pleurotus eryngii mushroom cultivating residues |
| CN104694513A (en) * | 2014-12-03 | 2015-06-10 | 江南大学 | Method for producing ligninase by using edible mushroom stick |
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2015
- 2015-07-01 CN CN201510385987.8A patent/CN105010725A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101812431A (en) * | 2009-02-23 | 2010-08-25 | 湖南省畜牧兽医研究所 | Method utilizing bacterisugar to prepare feed enzyme |
| CN102715339A (en) * | 2012-06-01 | 2012-10-10 | 陈华友 | Microorganism fodder production method based on pleurotus eryngii mushroom cultivating residues |
| CN104694513A (en) * | 2014-12-03 | 2015-06-10 | 江南大学 | Method for producing ligninase by using edible mushroom stick |
Non-Patent Citations (1)
| Title |
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| 黄碧芳等: "虎奶菇产纤维素酶条件优化的研究", 《中国食用菌》 * |
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Application publication date: 20151104 |
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