CN104968790A - 用于产生和选择转基因植物的方法和组合物 - Google Patents

用于产生和选择转基因植物的方法和组合物 Download PDF

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Publication number
CN104968790A
CN104968790A CN201380065324.7A CN201380065324A CN104968790A CN 104968790 A CN104968790 A CN 104968790A CN 201380065324 A CN201380065324 A CN 201380065324A CN 104968790 A CN104968790 A CN 104968790A
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China
Prior art keywords
plant
promoter
polynucleotide
polypeptide
seq
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CN201380065324.7A
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English (en)
Chinese (zh)
Inventor
M-J.乔
S.R.埃里斯
W.J.戈当-卡姆
Z-Y.赵
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C07F9/02—Phosphorus compounds
    • C07F9/28—Phosphorus compounds with one or more P—C bonds
    • C07F9/30—Phosphinic acids [R2P(=O)(OH)]; Thiophosphinic acids ; [R2P(=X1)(X2H) (X1, X2 are each independently O, S or Se)]
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    • C07F9/38—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)]
    • C07F9/3804—Phosphonic acids [RP(=O)(OH)2]; Thiophosphonic acids ; [RP(=X1)(X2H)2(X1, X2 are each independently O, S or Se)] not used, see subgroups
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CN201380065324.7A 2012-12-13 2013-12-11 用于产生和选择转基因植物的方法和组合物 Pending CN104968790A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261736947P 2012-12-13 2012-12-13
US61/736947 2012-12-13
US13/800447 2013-03-13
US13/800,447 US20140173775A1 (en) 2012-12-13 2013-03-13 Methods and compositions for producing and selecting transgenic plants
PCT/US2013/074379 WO2014093485A1 (en) 2012-12-13 2013-12-11 Methods and compositions for producing and selecting transgenic plants

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CN104968790A true CN104968790A (zh) 2015-10-07

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US (2) US20140173781A1 (https=)
EP (1) EP2931900A1 (https=)
JP (1) JP6383778B2 (https=)
CN (1) CN104968790A (https=)
AU (1) AU2013359340A1 (https=)
CA (1) CA2892533A1 (https=)
IL (2) IL238608A0 (https=)
RU (1) RU2015127989A (https=)
SA (1) SA515361041B1 (https=)
WO (3) WO2014093485A1 (https=)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110709519A (zh) * 2017-03-31 2020-01-17 先锋国际良种公司 表达调控元件及其用途
CN110950943A (zh) * 2019-12-30 2020-04-03 上海交通大学 紫花苜蓿‘WL525’胚胎发育晚期蛋白MsLEA-D34及其编码基因
CN111868247A (zh) * 2018-03-12 2020-10-30 先锋国际良种公司 用于植物转化的方法
CN114096672A (zh) * 2019-08-12 2022-02-25 先正达农作物保护股份公司 Wox基因
CN117757805A (zh) * 2023-12-25 2024-03-26 武汉艾迪晶生物科技有限公司 SbWOX基因和SbBBM基因在玉米育种的应用及玉米遗传转化方法
CN118109503A (zh) * 2024-04-16 2024-05-31 福建农林大学 大豆生长素酰胺合成酶gh3基因家族及其应用

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014004232A1 (en) * 2012-06-26 2014-01-03 Uop Llc Alkylation process using phosphonium-based ionic liquids
WO2016048891A1 (en) 2014-09-26 2016-03-31 Pioneer Hi-Bred International, Inc. Wheat ms1 polynucleotides, polypeptides, and mehtods of use
ES2881392T3 (es) 2015-10-30 2021-11-29 Pioneer Hi Bred Int Métodos y composiciones para transformación de plantas rápida
EP3550958A4 (en) * 2016-12-08 2020-10-07 Syngenta Participations AG PROCESSES FOR IMPROVING THE FREQUENCY OF TRANSFORMATION
BR112019018059A2 (pt) 2017-03-07 2020-08-04 BASF Agricultural Solutions Seed US LLC molécula de ácido nucleico recombinante, célula hospedeira, plantas, sementes transgênicas, polipeptídeo recombinante, método para produzir um polipeptídeo, método de controle de ervas daninhas, uso do ácido nucleico e produto de utilidade
CA3055389A1 (en) 2017-03-07 2018-09-13 BASF Agricultural Solutions Seed US LLC Hppd variants and methods of use
AU2018239406A1 (en) * 2017-03-23 2019-09-26 Basf Se Anther-specific promoter and uses thereof
WO2020005588A1 (en) * 2018-06-29 2020-01-02 BASF Agricultural Solutions Seed US LLC Herbicide tolerant plants expressing a cyanobacterial plastoquinone biosynthetic pathway
CN109212053B (zh) * 2018-07-07 2021-05-14 丁立平 一种测定饮用水中痕量草铵膦、草甘膦及氨甲基膦酸的气相色谱法
CN109666757B (zh) * 2019-01-31 2022-04-19 宁夏农林科学院农作物研究所（宁夏回族自治区农作物育种中心） 用于鉴定小麦春化基因vrn-d4的试剂盒及其专用成套引物对
US20220170033A1 (en) * 2019-03-27 2022-06-02 Pioneer Hi-Bred International, Inc. Plant explant transformation
CN110106200B (zh) * 2019-05-17 2021-02-02 中国农业科学院作物科学研究所 玉米bbm1基因在提高植物遗传转化效率中的应用
EP4268583A4 (en) * 2020-12-28 2025-01-08 Kaneka Corporation Method for producing transformed cells of corn or corn plant, nucleic acid construct, and method for introducing nucleic acid to cells or corn plant
US20250154522A1 (en) * 2021-11-02 2025-05-15 University Of Florida Research Foundation, Incorporated Improved genetic transformation by improving de-novo shoot regeneration in adult plants and during in-vitro tissue culture
JP2025512370A (ja) * 2022-04-12 2025-04-17 モンサント テクノロジー エルエルシー 自己切り出しのための植物調節エレメント及びその使用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110167516A1 (en) * 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
CN102159718A (zh) * 2008-09-15 2011-08-17 辛根塔有限公司 除草剂耐受性植物

Family Cites Families (130)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US762264A (en) 1903-11-06 1904-06-07 Pierre C Waring Process of making felt cloth.
US5380831A (en) 1986-04-04 1995-01-10 Mycogen Plant Science, Inc. Synthetic insecticidal crystal protein gene
US5304732A (en) 1984-03-06 1994-04-19 Mgi Pharma, Inc. Herbicide resistance in plants
US5331107A (en) 1984-03-06 1994-07-19 Mgi Pharma, Inc. Herbicide resistance in plants
US4761373A (en) 1984-03-06 1988-08-02 Molecular Genetics, Inc. Herbicide resistance in plants
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
ATE57390T1 (de) 1986-03-11 1990-10-15 Plant Genetic Systems Nv Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen.
US5273894A (en) 1986-08-23 1993-12-28 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US5605011A (en) 1986-08-26 1997-02-25 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5013659A (en) 1987-07-27 1991-05-07 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5378824A (en) 1986-08-26 1995-01-03 E. I. Du Pont De Nemours And Company Nucleic acid fragment encoding herbicide resistant plant acetolactate synthase
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US4764440A (en) 1987-05-05 1988-08-16 Eveready Battery Company Low temperature molten compositions
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
JPH01258408A (ja) * 1988-04-08 1989-10-16 Asahi Glass Co Ltd 非水電解液エネルギー貯蔵装置
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5550318A (en) 1990-04-17 1996-08-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
EP0426641B1 (en) 1989-10-31 2000-09-13 Monsanto Company Promoter for transgenic plants
FR2659871B1 (fr) 1990-03-20 1992-06-05 Inst Francais Du Petrole Composition liquide non aqueuse a caractere ionique et son utilisation comme solvant. invention de mm. yves chauvin, dominique commereuc, isabelle guibard, andre hirschauer, helene olivier, lucien saussine.
EP0452269B1 (en) 1990-04-12 2002-10-09 Syngenta Participations AG Tissue-preferential promoters
PL295547A1 (https=) 1990-04-26 1992-10-05 Plant Genetic Systems Nv
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
JP3173784B2 (ja) 1990-06-25 2001-06-04 モンサント カンパニー グリホセート耐性植物
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5277905A (en) 1991-01-16 1994-01-11 Mycogen Corporation Coleopteran-active bacillus thuringiensis isolate
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
GB9114259D0 (en) 1991-07-02 1991-08-21 Ici Plc Plant derived enzyme and dna sequences
US5731180A (en) 1991-07-31 1998-03-24 American Cyanamid Company Imidazolinone resistant AHAS mutants
DE69227911T2 (de) 1991-08-02 1999-05-12 Kubota Corp., Tokio/Tokyo Neuer mikroorganismus und insektizid
CA2116449C (en) 1991-08-27 2005-04-05 Vaughan Alan Hilder Proteins with insecticidal properties against homopteran insects and their use in plant protection
CA2112999C (en) 1991-10-04 2007-04-24 Mark A. Conkling Pathogen-resistant transgenic plants
TW261517B (https=) 1991-11-29 1995-11-01 Mitsubishi Shozi Kk
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5428148A (en) 1992-04-24 1995-06-27 Beckman Instruments, Inc. N4 - acylated cytidinyl compounds useful in oligonucleotide synthesis
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
WO1994002620A2 (en) 1992-07-27 1994-02-03 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
DE69428290T2 (de) 1993-01-13 2002-04-18 Pioneer Hi-Bred International, Inc. Derivate von alpha-hordothionin mit höherem behalt an lysin
US5583210A (en) 1993-03-18 1996-12-10 Pioneer Hi-Bred International, Inc. Methods and compositions for controlling plant development
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5593881A (en) 1994-05-06 1997-01-14 Mycogen Corporation Bacillus thuringiensis delta-endotoxin
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5767373A (en) 1994-06-16 1998-06-16 Novartis Finance Corporation Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5792931A (en) 1994-08-12 1998-08-11 Pioneer Hi-Bred International, Inc. Fumonisin detoxification compositions and methods
US5659026A (en) 1995-03-24 1997-08-19 Pioneer Hi-Bred International ALS3 promoter
CA2218526C (en) 1995-04-20 2012-06-12 American Cyanamid Company Structure-based designed herbicide resistant products
US5853973A (en) 1995-04-20 1998-12-29 American Cyanamid Company Structure based designed herbicide resistant products
WO1996038563A1 (en) 1995-06-02 1996-12-05 Pioneer Hi-Bred International, Inc. HIGH METHIONINE DERIVATIVES OF α-HORDOTHIONIN
FR2734842B1 (fr) 1995-06-02 1998-02-27 Rhone Poulenc Agrochimie Sequence adn d'un gene de l'hydroxy-phenyl pyruvate dioxygenase et obtention de plantes contenant un gene de l'hydroxy-phenyl pyruvate dioxygenase, tolerantes a certains herbicides
WO1996038562A1 (en) 1995-06-02 1996-12-05 Pioneer Hi-Bred International, Inc. HIGH THREONINE DERIVATIVES OF α-HORDOTHIONIN
DK0937098T3 (da) 1995-06-07 2002-12-02 Invitrogen Corp Rekombinatorisk kloning under anvendelse af gensplejsede rekombinationssites
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US5737514A (en) 1995-11-29 1998-04-07 Texas Micro, Inc. Remote checkpoint memory system and protocol for fault-tolerant computer system
US5703049A (en) 1996-02-29 1997-12-30 Pioneer Hi-Bred Int'l, Inc. High methionine derivatives of α-hordothionin for pathogen-control
AUPN903196A0 (en) * 1996-03-29 1996-04-26 Australian National University, The Single-step excision means
US6175056B1 (en) 1996-04-29 2001-01-16 The Procter & Gamble Company Disposable absorbent article having an expanding topsheet and being capable of self-shaping in use
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
US5824832A (en) 1996-07-22 1998-10-20 Akzo Nobel Nv Linear alxylbenzene formation using low temperature ionic liquid
JP3441899B2 (ja) 1996-11-01 2003-09-02 理化学研究所 完全長ｃＤＮＡライブラリーの作成方法
DE19652284A1 (de) 1996-12-16 1998-06-18 Hoechst Schering Agrevo Gmbh Neue Gene codierend für Aminosäure-Deacetylasen mit Spezifität für N-Acetyl-L-Phosphinothricin, ihre Isolierung und Verwendung
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
WO1998056238A1 (en) 1997-06-11 1998-12-17 The United States Of America, As Represented By The Secretary Of Agriculture Transformation of wheat with the cyanamide hydratase gene
WO1999011807A1 (en) * 1997-09-05 1999-03-11 Purdue Research Foundation Selective expression of genes in plants
US6245968B1 (en) 1997-11-07 2001-06-12 Aventis Cropscience S.A. Mutated hydroxyphenylpyruvate dioxygenase, DNA sequence and isolation of plants which contain such a gene and which are tolerant to herbicides
US6069115A (en) 1997-11-12 2000-05-30 Rhone-Poulenc Agrochimie Method of controlling weeds in transgenic crops
JP4206154B2 (ja) 1997-11-13 2009-01-07 大日本住友製薬株式会社 変異型ｌｏｘＰ配列とその応用
EP1034286B1 (en) 1997-11-18 2010-01-06 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems
AU1526199A (en) 1997-11-18 1999-06-07 Pioneer Hi-Bred International, Inc. Targeted manipulation of herbicide-resistance genes in plants
US5929301A (en) 1997-11-18 1999-07-27 Pioneer Hi-Bred International Nucleic acid sequence encoding FLP recombinase
CA2306188C (en) 1997-11-18 2008-08-05 Pioneer Hi-Bred International, Inc. A novel method for the integration of foreign dna into eukaryotic genomes
US6187994B1 (en) 1997-11-18 2001-02-13 Pioneer Hi-Bred International, Inc. Compositions and methods for genetic modification of plants
CA2315546C (en) 1998-02-26 2008-04-29 Pioneer Hi-Bred International, Inc. Constitutive maize promoters
WO1999043819A1 (en) 1998-02-26 1999-09-02 Pioneer Hi-Bred International, Inc. Family of maize pr-1 genes and promoters
US6070283A (en) 1998-07-10 2000-06-06 Rite-Hite Holding Corporation Dock leveler with integral bumpers
AU5489099A (en) 1998-08-20 2000-03-14 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
AU5788299A (en) 1998-08-28 2000-03-21 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from (end) genes
EP1131454A2 (en) 1998-11-09 2001-09-12 Pioneer Hi-Bred International, Inc. Transcriptional activator nucleic acids, polypeptides and methods of use thereof
US6890726B1 (en) 1999-04-06 2005-05-10 Oklahoma Medical Research Foundation Method for selecting recombinase variants with altered specificity
JP4808880B2 (ja) 1999-07-23 2011-11-02 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア バクテリオファージｐｈｉｃ３１組み換え系による真核細胞におけるｄｎａ組み換え
AU6529900A (en) 1999-08-09 2001-03-05 Monsanto Technology Llc Novel cloning methods and vectors
EP1200611A1 (en) 1999-08-13 2002-05-02 Syngenta Participations AG Herbicide-tolerant protoporphyrinogen oxidase
WO2001023575A2 (en) 1999-09-30 2001-04-05 E.I. Du Pont De Nemours And Company Wuschel (wus) gene homologs
JP4787440B2 (ja) 1999-09-30 2011-10-05 泉 斎藤 変異型ｆｒｔ配列を含有してなるｄｎａ
US7256322B2 (en) 1999-10-01 2007-08-14 Pioneer Hi-Bred International, Inc. Wuschel (WUS) Gene Homologs
AU2001277520B2 (en) 2000-06-28 2006-08-03 Sungene Gmbh & Co. Kgaa Binary vectors for improved transformation of plant systems
US6512165B1 (en) 2000-07-10 2003-01-28 Pioneer Hi-Bred International, Inc. Methods for enhancing plant transformation frequencies
US7462481B2 (en) 2000-10-30 2008-12-09 Verdia, Inc. Glyphosate N-acetyltransferase (GAT) genes
WO2002036782A2 (en) 2000-10-30 2002-05-10 Maxygen, Inc. Novel glyphosate n-acetyltransferase (gat) genes
US7294759B2 (en) 2001-06-29 2007-11-13 E. I. Du Pont De Nemours And Company Alteration of oil traits in plants
AT5356U1 (de) 2001-07-16 2002-06-25 Uv & P Umweltman Verfahrenstec Verfahren zur immobilisierung von verbrennungsrückständen
EP1458875A2 (de) 2001-12-20 2004-09-22 Sungene GmbH & Co. KGaA Verfahren zur transformation von pflanzlichen plastiden
CA2422480A1 (en) 2002-03-15 2003-09-15 National Research Council Of Canada Promoters for molecular farming and/or early detection of transgenic plants
US7238854B2 (en) 2002-04-11 2007-07-03 E. I. Du Pont De Nemours And Company Method of controlling site-specific recombination
WO2003092360A2 (en) 2002-04-30 2003-11-13 Verdia, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
US20040203141A1 (en) 2003-04-11 2004-10-14 Jorge Dubcovsky Use of the AP1 gene promoter to control the vernalization response and flowering time in grasses
CN1863914B (zh) 2003-04-29 2011-03-09 先锋高级育种国际公司 新的草甘膦-n-乙酰转移酶(gat)基因
US8329603B2 (en) * 2003-09-16 2012-12-11 Uop Llc Isoparaffin-olefin alkylation
CA2554644C (en) 2004-02-02 2015-06-16 Pioneer Hi-Bred International, Inc. Ap2 domain transcription factor odp2 (ovule development protein 2) and methods of use
US7253000B2 (en) 2004-04-26 2007-08-07 Pioneer Hi-Bred International, Inc. Transcriptional activators involved in abiotic stress tolerance
US7405074B2 (en) 2004-04-29 2008-07-29 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GAT) genes
US7473822B1 (en) 2005-06-27 2009-01-06 Iowa State University Research Foundation, Inc. Soybean transformation and regeneration using half-seed explant
BRPI0612862A2 (pt) 2005-07-18 2010-11-30 Pioneer Hi Bred Int polinucleotìdeo isolado, método para obtenção de uma célula transformada, método para obtenção de uma planta transformada, método de produção de recombinação de dna de sìtio especìfico, biblioteca isolada, método de geração de biblioteca de moléculas e método para selecionar sìtio de recombinação frt modificado
MEP3508A (xx) 2005-08-24 2010-02-10 Pioneer Hi Bred Int Kompozicije koje obezbjeđuju otpornost na više hibricida i njihov postupak upotrebe
US7491813B2 (en) 2005-12-07 2009-02-17 Monsanto Technology Llc Promoter polynucleotides identified from Zea mays for use in plants
US7390928B2 (en) * 2005-12-14 2008-06-24 Chemtura Corporation Alkylation of N′-phenyl-N-alkylphenylenediamines in ionic liquid, N′-phenyl-N-alkyl (alkylphenylene) diamines produced thereby, and lubricant compositions containing same
US7569740B2 (en) * 2005-12-20 2009-08-04 Chevron U.S.A. Inc. Alkylation of olefins with isoparaffins in ionic liquid to make lubricant or fuel blendstock
US7928296B2 (en) 2006-10-30 2011-04-19 Pioneer Hi-Bred International, Inc. Maize event DP-098140-6 and compositions and methods for the identification and/or detection thereof
GB2447416B (en) * 2006-12-15 2010-08-25 Uni I Stavanger Methods and vectors for transformation of plastids and marker excision using site-specific recombination
US8710206B2 (en) 2007-06-15 2014-04-29 E. I. Du Pont De Nemours And Company Soybean EF1A promoter and its use in constitutive expression of transgenic genes in plants
CN101284822A (zh) * 2008-06-06 2008-10-15 福建师范大学 一种制备环氧脂肪酸二元醇酯的方法
CN102264758B (zh) 2008-10-28 2015-04-22 先锋国际良种公司 磺酰脲-响应性的阻遏蛋白
WO2010121316A1 (en) * 2009-04-24 2010-10-28 Australian Centre For Plant Functional Genomics Pty Ltd Drought responsive expression of genes from the zea mays rab17 promoter
US8581046B2 (en) 2010-11-24 2013-11-12 Pioneer Hi-Bred International, Inc. Brassica gat event DP-073496-4 and compositions and methods for the identification and/or detection thereof
US20120136189A1 (en) * 2010-03-09 2012-05-31 Conocophillips Company - Ip Services Group Buffered ionic liquids for olefin dimerization
EP2560473A1 (en) * 2010-04-23 2013-02-27 Pioneer Hi-Bred International Inc. Gene switch compositions and methods of use
CN101979143B (zh) * 2010-09-15 2012-07-04 湖南科茂林化有限公司 一种松香或其衍生物的酯化催化剂及其应用
JP6235470B2 (ja) * 2011-06-17 2017-11-22 フルイディック, インク.Fluidic, Inc. ジアルキルカーボネートの四級化を利用するヘテロイオン化合物の合成
AU2012301912A1 (en) * 2011-08-31 2014-03-06 E. I. Dupont De Nemours & Company Methods for tissue culture and transformation of sugarcane
WO2014004232A1 (en) * 2012-06-26 2014-01-03 Uop Llc Alkylation process using phosphonium-based ionic liquids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102159718A (zh) * 2008-09-15 2011-08-17 辛根塔有限公司 除草剂耐受性植物
US20110167516A1 (en) * 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HEE-JONG WOO ET AL.: "Auto-excision of selectable marker genes from transgenic tobacco via a stress inducible FLP/FRT site-specific recombination system", 《TRANSGENIC RESEARCH》 *
杨朝国等: "抗除草剂草丁膦转基因玉米后代筛选方法的研究", 《作物杂志》 *

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110709519A (zh) * 2017-03-31 2020-01-17 先锋国际良种公司 表达调控元件及其用途
CN110709519B (zh) * 2017-03-31 2023-09-15 先锋国际良种公司 表达调控元件及其用途
US11814637B2 (en) 2017-03-31 2023-11-14 Pioneer Hi-Bred International, Inc Expression modulating elements and use thereof
CN111868247A (zh) * 2018-03-12 2020-10-30 先锋国际良种公司 用于植物转化的方法
CN114096672A (zh) * 2019-08-12 2022-02-25 先正达农作物保护股份公司 Wox基因
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CN110950943A (zh) * 2019-12-30 2020-04-03 上海交通大学 紫花苜蓿‘WL525’胚胎发育晚期蛋白MsLEA-D34及其编码基因
CN110950943B (zh) * 2019-12-30 2021-09-17 上海交通大学 紫花苜蓿‘WL525’胚胎发育晚期蛋白MsLEA-D34及其编码基因
CN117757805A (zh) * 2023-12-25 2024-03-26 武汉艾迪晶生物科技有限公司 SbWOX基因和SbBBM基因在玉米育种的应用及玉米遗传转化方法
CN118109503A (zh) * 2024-04-16 2024-05-31 福建农林大学 大豆生长素酰胺合成酶gh3基因家族及其应用

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