CN104962574A - 一种节杆菌表达质粒与应用 - Google Patents
一种节杆菌表达质粒与应用 Download PDFInfo
- Publication number
- CN104962574A CN104962574A CN201510316768.4A CN201510316768A CN104962574A CN 104962574 A CN104962574 A CN 104962574A CN 201510316768 A CN201510316768 A CN 201510316768A CN 104962574 A CN104962574 A CN 104962574A
- Authority
- CN
- China
- Prior art keywords
- plasmid
- arthrobacter
- sequence
- gene
- application
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000013613 expression plasmid Substances 0.000 title claims abstract description 17
- 241000186073 Arthrobacter sp. Species 0.000 title abstract 6
- 239000013612 plasmid Substances 0.000 claims abstract description 90
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 26
- 239000002773 nucleotide Substances 0.000 claims abstract description 11
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 11
- 241000186063 Arthrobacter Species 0.000 claims description 59
- 238000010367 cloning Methods 0.000 abstract description 8
- 241000588724 Escherichia coli Species 0.000 abstract description 4
- 230000003362 replicative effect Effects 0.000 abstract 2
- 229930027917 kanamycin Natural products 0.000 abstract 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 abstract 1
- 229960000318 kanamycin Drugs 0.000 abstract 1
- 229930182823 kanamycin A Natural products 0.000 abstract 1
- 241000894006 Bacteria Species 0.000 description 20
- 239000012634 fragment Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 9
- 238000010276 construction Methods 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 6
- 101000833492 Homo sapiens Jouberin Proteins 0.000 description 5
- 101000651236 Homo sapiens NCK-interacting protein with SH3 domain Proteins 0.000 description 5
- 102100024407 Jouberin Human genes 0.000 description 5
- 230000014509 gene expression Effects 0.000 description 5
- 239000003550 marker Substances 0.000 description 5
- 108090000790 Enzymes Proteins 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 101710084218 Master replication protein Proteins 0.000 description 4
- 101710112078 Para-Rep C2 Proteins 0.000 description 4
- 102100022881 Rab proteins geranylgeranyltransferase component A 1 Human genes 0.000 description 4
- 102100022880 Rab proteins geranylgeranyltransferase component A 2 Human genes 0.000 description 4
- 102100026503 Sperm mitochondrial-associated cysteine-rich protein Human genes 0.000 description 4
- 101710119961 Trans-acting factor C Proteins 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 101710112083 Para-Rep C1 Proteins 0.000 description 3
- 108020005091 Replication Origin Proteins 0.000 description 3
- 101710119887 Trans-acting factor B Proteins 0.000 description 3
- 230000004913 activation Effects 0.000 description 3
- 125000003275 alpha amino acid group Chemical group 0.000 description 3
- XUWPJKDMEZSVTP-LTYMHZPRSA-N kalafungina Chemical compound O=C1C2=C(O)C=CC=C2C(=O)C2=C1[C@@H](C)O[C@H]1[C@@H]2OC(=O)C1 XUWPJKDMEZSVTP-LTYMHZPRSA-N 0.000 description 3
- 230000009466 transformation Effects 0.000 description 3
- 241000186226 Corynebacterium glutamicum Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 2
- 241000192017 Micrococcaceae Species 0.000 description 2
- 102000005877 Peptide Initiation Factors Human genes 0.000 description 2
- 108010044843 Peptide Initiation Factors Proteins 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000005090 green fluorescent protein Substances 0.000 description 2
- 230000001788 irregular Effects 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 210000002706 plastid Anatomy 0.000 description 2
- 230000010076 replication Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 1
- SNICXCGAKADSCV-JTQLQIEISA-N (-)-Nicotine Chemical compound CN1CCC[C@H]1C1=CC=CN=C1 SNICXCGAKADSCV-JTQLQIEISA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 244000153158 Ammi visnaga Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 241001233910 Arthrobacter rhombi Species 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000186427 Cutibacterium acnes Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
- 102000018251 Hypoxanthine Phosphoribosyltransferase Human genes 0.000 description 1
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 101710108890 Rab proteins geranylgeranyltransferase component A 1 Proteins 0.000 description 1
- 108700005075 Regulator Genes Proteins 0.000 description 1
- 235000003976 Ruta Nutrition 0.000 description 1
- 240000005746 Ruta graveolens Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 229930003756 Vitamin B7 Natural products 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- GZCGUPFRVQAUEE-SLPGGIOYSA-N aldehydo-D-glucose Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O GZCGUPFRVQAUEE-SLPGGIOYSA-N 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003876 biosurfactant Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 239000005457 ice water Substances 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000029052 metamorphosis Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 229960002715 nicotine Drugs 0.000 description 1
- SNICXCGAKADSCV-UHFFFAOYSA-N nicotine Natural products CN1CCCC1C1=CC=CN=C1 SNICXCGAKADSCV-UHFFFAOYSA-N 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 229940055019 propionibacterium acne Drugs 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 235000005806 ruta Nutrition 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 239000011735 vitamin B7 Substances 0.000 description 1
- 235000011912 vitamin B7 Nutrition 0.000 description 1
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (9)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510316768.4A CN104962574B (zh) | 2015-06-10 | 2015-06-10 | 一种节杆菌表达质粒与应用 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510316768.4A CN104962574B (zh) | 2015-06-10 | 2015-06-10 | 一种节杆菌表达质粒与应用 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104962574A true CN104962574A (zh) | 2015-10-07 |
CN104962574B CN104962574B (zh) | 2017-11-24 |
Family
ID=54216664
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510316768.4A Active CN104962574B (zh) | 2015-06-10 | 2015-06-10 | 一种节杆菌表达质粒与应用 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104962574B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105505933A (zh) * | 2016-01-07 | 2016-04-20 | 南京工业大学 | 新型节杆菌启动子序列及其应用 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH022378A (ja) * | 1988-03-09 | 1990-01-08 | Kyowa Hakko Kogyo Co Ltd | プラスミドpBP1 |
CN102268385A (zh) * | 2010-06-04 | 2011-12-07 | 南京工业大学 | 一种用于发酵生产环磷酸腺苷的节杆菌及其应用 |
CN103320373A (zh) * | 2013-06-20 | 2013-09-25 | 南京工业大学 | 一株过表达次黄嘌呤磷酸核糖转移酶基因的节杆菌及其构建方法与应用 |
-
2015
- 2015-06-10 CN CN201510316768.4A patent/CN104962574B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH022378A (ja) * | 1988-03-09 | 1990-01-08 | Kyowa Hakko Kogyo Co Ltd | プラスミドpBP1 |
CN102268385A (zh) * | 2010-06-04 | 2011-12-07 | 南京工业大学 | 一种用于发酵生产环磷酸腺苷的节杆菌及其应用 |
CN103320373A (zh) * | 2013-06-20 | 2013-09-25 | 南京工业大学 | 一株过表达次黄嘌呤磷酸核糖转移酶基因的节杆菌及其构建方法与应用 |
Non-Patent Citations (3)
Title |
---|
EMMANUEL F. MONGODIN ET AL.: "Secrets of Soil Survival Reof Arthrobacter aurescens TC1vealed by the Genome Sequence of Arthrobacter aurescens TC1", 《PLOS GENETICS》 * |
GROSSE,C.: "Arthrobacter sp. plasmid pA3,Accession NO:AJ131246.1", 《GENBANK》 * |
HEIKO NIEWERTH ET AL.: "Complete genome sequence and metabolic potential of the quinaldine-degrading bacterium Arthrobacter sp. Rue61a", 《BMC GENOMICS》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105505933A (zh) * | 2016-01-07 | 2016-04-20 | 南京工业大学 | 新型节杆菌启动子序列及其应用 |
CN105505933B (zh) * | 2016-01-07 | 2019-03-01 | 南京工业大学 | 新型节杆菌启动子序列及其应用 |
Also Published As
Publication number | Publication date |
---|---|
CN104962574B (zh) | 2017-11-24 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110358720B (zh) | 一种生产异丁醇的运动发酵单胞菌重组菌株、构建方法及其应用 | |
CN108753636A (zh) | 一种生产酪醇及羟基酪醇的酵母及构建方法 | |
CN110055204B (zh) | 一种敲除spoⅡQ和pcf基因提高地衣芽孢杆菌发酵产酶的方法及应用 | |
CN110157654B (zh) | 一种纳豆芽孢杆菌重组菌及其构建方法与应用 | |
CN113881586B (zh) | 耐高温、耐高糖、耐高盐酿酒酵母菌株及构建方法和应用 | |
CN105420154A (zh) | 双基因敲除重组红球菌、构建方法及其应用 | |
CN105821071A (zh) | 一种基于同源重组的乳酸片球菌dq2的无标记基因敲除方法 | |
CN113604472B (zh) | 一种应用于里氏木霉的CRISPR/Cas基因编辑系统 | |
CN101748069A (zh) | 一种重组蓝藻 | |
CN116286560B (zh) | 一株拉乌尔菌hc6及其低温生产2,3-丁二醇的应用 | |
CN103131718B (zh) | 来源产甘油假丝酵母新型耐高渗功能基因CgHog1的克隆及其应用 | |
CN112280700A (zh) | 一株耐乙酸和甲酸的发酵菌株及其构建方法 | |
CN107937296A (zh) | 一种具有乙酸糠醛香草醛耐受性重组酿酒酵母及制备方法、应用 | |
CN106906238B (zh) | 一种链霉菌抗生素生物合成基因簇多拷贝扩增方法及应用 | |
WO2020134427A1 (zh) | sll0528基因在提高集胞藻PCC6803乙醇耐受性中的应用 | |
CN104962574A (zh) | 一种节杆菌表达质粒与应用 | |
CN114540395B (zh) | 希瓦氏菌中木糖利用代谢的构建方法 | |
CN103232994B (zh) | 一种筛选嗜酸性氧化硫硫杆菌无标记基因敲除菌株的方法 | |
CN104204206B (zh) | 一种用于生产丁醇的方法 | |
CN115806922A (zh) | 运动发酵单胞菌的基因工程菌株及其应用 | |
CN105602914B (zh) | 一种来源于马克斯克鲁维酵母的烷基过氧化物还原酶和硫氧还蛋白还原酶及其应用 | |
CN104403956B (zh) | 木糖醇高温高产工程菌株的构建及应用 | |
CN101525580B (zh) | 一种双倍体组氨酸营养缺陷型酿酒酵母及其构建方法 | |
CN109370972A (zh) | 一种醋酸杆菌工程菌及其应用 | |
CN104131023A (zh) | 运动发酵单胞菌red重组系统表达质粒及其构建方法与应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20180523 Address after: 211800 room 811, building B, phase 1, China Dandan Ecological Life Science Park, No. 3-1, new Kumho Road, Jiangbei new district, Nanjing, Jiangsu Patentee after: Nanjing Interesting Enzyme Biotechnology Co.,Ltd. Address before: 210009, No. 5, new exemplary Road, Nanjing, Jiangsu Patentee before: Nanjing University of Technology |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20190306 Address after: 210000 Zhongdanyuan, No. 3-1 Xinjinhu Road, Jiangbei New District, Nanjing City, Jiangsu Province Co-patentee after: Nanjing Intelligent Enzyme Biotechnology Co.,Ltd. Patentee after: Nanjing Interesting Enzyme Biotechnology Co.,Ltd. Co-patentee after: Shanghai Ren Enzyme Biotechnology Co.,Ltd. Address before: 211800 room 811, building B, phase 1, China Dandan Ecological Life Science Park, No. 3-1, new Kumho Road, Jiangbei new district, Nanjing, Jiangsu Patentee before: Nanjing Interesting Enzyme Biotechnology Co.,Ltd. |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20221028 Address after: 210000 Zhongdanyuan, No. 3-1 Xinjinhu Road, Jiangbei New District, Nanjing City, Jiangsu Province Patentee after: Nanjing Interesting Enzyme Biotechnology Co.,Ltd. Patentee after: Shanghai Ren Enzyme Biotechnology Co.,Ltd. Address before: 210000 Zhongdanyuan, No. 3-1 Xinjinhu Road, Jiangbei New District, Nanjing City, Jiangsu Province Patentee before: Nanjing Interesting Enzyme Biotechnology Co.,Ltd. Patentee before: Nanjing Intelligent Enzyme Biotechnology Co.,Ltd. Patentee before: Shanghai Ren Enzyme Biotechnology Co.,Ltd. |