CN104940994B - 一种人造鼻粘膜及其用途 - Google Patents
一种人造鼻粘膜及其用途 Download PDFInfo
- Publication number
- CN104940994B CN104940994B CN201510412461.4A CN201510412461A CN104940994B CN 104940994 B CN104940994 B CN 104940994B CN 201510412461 A CN201510412461 A CN 201510412461A CN 104940994 B CN104940994 B CN 104940994B
- Authority
- CN
- China
- Prior art keywords
- pyrrolidone
- acid
- carboxylic acid
- group
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 210000002850 nasal mucosa Anatomy 0.000 title claims abstract description 48
- 229950008882 polysorbate Drugs 0.000 claims abstract description 56
- 229920000136 polysorbate Polymers 0.000 claims abstract description 56
- ODHCTXKNWHHXJC-VKHMYHEASA-N 5-oxo-L-proline Chemical class OC(=O)[C@@H]1CCC(=O)N1 ODHCTXKNWHHXJC-VKHMYHEASA-N 0.000 claims abstract description 11
- ODHCTXKNWHHXJC-UHFFFAOYSA-N 5-oxoproline Chemical compound OC(=O)C1CCC(=O)N1 ODHCTXKNWHHXJC-UHFFFAOYSA-N 0.000 claims description 34
- 238000002360 preparation method Methods 0.000 claims description 20
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 19
- 239000011591 potassium Substances 0.000 claims description 19
- 229910052700 potassium Inorganic materials 0.000 claims description 18
- -1 poly- L- GLUs Polymers 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000011575 calcium Substances 0.000 claims description 9
- 229910052791 calcium Inorganic materials 0.000 claims description 9
- 239000002253 acid Substances 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 7
- OWVLYQRCCIEOPF-QHTZZOMLSA-L zinc;(2s)-5-oxopyrrolidine-2-carboxylate Chemical compound [Zn+2].[O-]C(=O)[C@@H]1CCC(=O)N1.[O-]C(=O)[C@@H]1CCC(=O)N1 OWVLYQRCCIEOPF-QHTZZOMLSA-L 0.000 claims description 7
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical group OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 6
- 239000002585 base Substances 0.000 claims description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 239000003607 modifier Substances 0.000 claims description 6
- 230000003204 osmotic effect Effects 0.000 claims description 6
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical group Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims description 4
- 244000246386 Mentha pulegium Species 0.000 claims description 4
- 235000016257 Mentha pulegium Nutrition 0.000 claims description 4
- 235000004357 Mentha x piperita Nutrition 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 150000002148 esters Chemical class 0.000 claims description 4
- 235000001050 hortel pimenta Nutrition 0.000 claims description 4
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 claims description 4
- 229920001661 Chitosan Polymers 0.000 claims description 3
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims description 3
- 229920002153 Hydroxypropyl cellulose Polymers 0.000 claims description 3
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 3
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 claims description 3
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 3
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 claims description 3
- 229930195725 Mannitol Natural products 0.000 claims description 3
- 229960004308 acetylcysteine Drugs 0.000 claims description 3
- 238000009472 formulation Methods 0.000 claims description 3
- 235000011187 glycerol Nutrition 0.000 claims description 3
- 239000001863 hydroxypropyl cellulose Substances 0.000 claims description 3
- 235000010977 hydroxypropyl cellulose Nutrition 0.000 claims description 3
- 229920003088 hydroxypropyl methyl cellulose Polymers 0.000 claims description 3
- 235000010979 hydroxypropyl methyl cellulose Nutrition 0.000 claims description 3
- 239000004615 ingredient Substances 0.000 claims description 3
- 229910052744 lithium Inorganic materials 0.000 claims description 3
- 229910052748 manganese Inorganic materials 0.000 claims description 3
- 239000011572 manganese Substances 0.000 claims description 3
- 239000000594 mannitol Substances 0.000 claims description 3
- 235000010355 mannitol Nutrition 0.000 claims description 3
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims description 3
- 239000011734 sodium Substances 0.000 claims description 3
- 229910052708 sodium Inorganic materials 0.000 claims description 3
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims description 2
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 claims description 2
- 102000008186 Collagen Human genes 0.000 claims description 2
- 108010035532 Collagen Proteins 0.000 claims description 2
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 2
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 2
- 239000002202 Polyethylene glycol Substances 0.000 claims description 2
- 108010039918 Polylysine Proteins 0.000 claims description 2
- 229920002125 Sokalan® Polymers 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 2
- 239000000783 alginic acid Substances 0.000 claims description 2
- 229920000615 alginic acid Polymers 0.000 claims description 2
- 235000010443 alginic acid Nutrition 0.000 claims description 2
- 229960001126 alginic acid Drugs 0.000 claims description 2
- 150000004781 alginic acids Chemical class 0.000 claims description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- 235000011114 ammonium hydroxide Nutrition 0.000 claims description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- 229920001436 collagen Polymers 0.000 claims description 2
- 239000008103 glucose Substances 0.000 claims description 2
- 229920002674 hyaluronan Polymers 0.000 claims description 2
- 229960003160 hyaluronic acid Drugs 0.000 claims description 2
- 239000011777 magnesium Substances 0.000 claims description 2
- 229910052749 magnesium Inorganic materials 0.000 claims description 2
- 229920002401 polyacrylamide Polymers 0.000 claims description 2
- 239000004584 polyacrylic acid Substances 0.000 claims description 2
- 229920001223 polyethylene glycol Polymers 0.000 claims description 2
- 229920000656 polylysine Polymers 0.000 claims description 2
- 150000003233 pyrroles Chemical class 0.000 claims description 2
- 229940054269 sodium pyruvate Drugs 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 239000011975 tartaric acid Substances 0.000 claims description 2
- 235000002906 tartaric acid Nutrition 0.000 claims description 2
- 239000003937 drug carrier Substances 0.000 claims 2
- 239000003513 alkali Substances 0.000 claims 1
- DNSISZSEWVHGLH-UHFFFAOYSA-N butanamide Chemical compound CCCC(N)=O DNSISZSEWVHGLH-UHFFFAOYSA-N 0.000 claims 1
- 125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 claims 1
- 229940045110 chitosan Drugs 0.000 claims 1
- 238000005660 chlorination reaction Methods 0.000 claims 1
- 229960005188 collagen Drugs 0.000 claims 1
- 230000003750 conditioning effect Effects 0.000 claims 1
- 125000001436 propyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])[H] 0.000 claims 1
- 239000003223 protective agent Substances 0.000 claims 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 18
- 201000010099 disease Diseases 0.000 abstract description 17
- 230000002980 postoperative effect Effects 0.000 abstract description 16
- 241000712461 unidentified influenza virus Species 0.000 abstract description 15
- 206010022000 influenza Diseases 0.000 abstract description 13
- 206010039085 Rhinitis allergic Diseases 0.000 abstract description 11
- 201000010105 allergic rhinitis Diseases 0.000 abstract description 11
- 239000000463 material Substances 0.000 abstract description 11
- 230000006378 damage Effects 0.000 abstract description 8
- 230000035876 healing Effects 0.000 abstract description 8
- 230000001681 protective effect Effects 0.000 abstract description 7
- 239000000370 acceptor Substances 0.000 abstract description 6
- 238000006243 chemical reaction Methods 0.000 abstract description 4
- 230000000474 nursing effect Effects 0.000 abstract description 2
- 238000005728 strengthening Methods 0.000 abstract description 2
- 238000012360 testing method Methods 0.000 description 56
- 210000004027 cell Anatomy 0.000 description 38
- 241000700605 Viruses Species 0.000 description 30
- 210000001331 nose Anatomy 0.000 description 26
- 238000002474 experimental method Methods 0.000 description 23
- 210000004400 mucous membrane Anatomy 0.000 description 19
- 238000011282 treatment Methods 0.000 description 19
- 230000014509 gene expression Effects 0.000 description 18
- 230000000694 effects Effects 0.000 description 15
- 239000003814 drug Substances 0.000 description 13
- 210000002966 serum Anatomy 0.000 description 13
- 235000002639 sodium chloride Nutrition 0.000 description 12
- 239000007788 liquid Substances 0.000 description 11
- 210000003928 nasal cavity Anatomy 0.000 description 11
- 230000003612 virological effect Effects 0.000 description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 9
- 230000000840 anti-viral effect Effects 0.000 description 9
- 206010039083 rhinitis Diseases 0.000 description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 8
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- 208000015181 infectious disease Diseases 0.000 description 8
- 238000000034 method Methods 0.000 description 8
- 241000709661 Enterovirus Species 0.000 description 7
- 239000012679 serum free medium Substances 0.000 description 7
- 230000001629 suppression Effects 0.000 description 7
- 230000004083 survival effect Effects 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 6
- 238000003556 assay Methods 0.000 description 6
- 108020004999 messenger RNA Proteins 0.000 description 6
- 229940079593 drug Drugs 0.000 description 5
- 239000011780 sodium chloride Substances 0.000 description 5
- 239000000243 solution Substances 0.000 description 5
- 208000024891 symptom Diseases 0.000 description 5
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- 206010061218 Inflammation Diseases 0.000 description 4
- 150000001732 carboxylic acid derivatives Chemical class 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 238000004821 distillation Methods 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 230000001965 increasing effect Effects 0.000 description 4
- 230000004054 inflammatory process Effects 0.000 description 4
- 210000004379 membrane Anatomy 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 231100001274 therapeutic index Toxicity 0.000 description 4
- 231100000816 toxic dose Toxicity 0.000 description 4
- 241000321096 Adenoides Species 0.000 description 3
- 206010002091 Anaesthesia Diseases 0.000 description 3
- 108010019160 Pancreatin Proteins 0.000 description 3
- 229930182555 Penicillin Natural products 0.000 description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 3
- 208000037062 Polyps Diseases 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 210000002534 adenoid Anatomy 0.000 description 3
- 230000037005 anaesthesia Effects 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000001684 chronic effect Effects 0.000 description 3
- 210000004081 cilia Anatomy 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 239000003995 emulsifying agent Substances 0.000 description 3
- 238000000684 flow cytometry Methods 0.000 description 3
- 229960003088 loratadine Drugs 0.000 description 3
- JCCNYMKQOSZNPW-UHFFFAOYSA-N loratadine Chemical compound C1CN(C(=O)OCC)CCC1=C1C2=NC=CC=C2CCC2=CC(Cl)=CC=C21 JCCNYMKQOSZNPW-UHFFFAOYSA-N 0.000 description 3
- 238000001543 one-way ANOVA Methods 0.000 description 3
- 229940055695 pancreatin Drugs 0.000 description 3
- 229940049954 penicillin Drugs 0.000 description 3
- 239000002504 physiological saline solution Substances 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 230000008961 swelling Effects 0.000 description 3
- 231100000331 toxic Toxicity 0.000 description 3
- 230000002588 toxic effect Effects 0.000 description 3
- 125000000070 5-oxo-L-proline group Chemical class [H]N1[C@@](C(=O)[*])([H])C([H])([H])C([H])([H])C1=O 0.000 description 2
- 238000011725 BALB/c mouse Methods 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- VZCYOOQTPOCHFL-OWOJBTEDSA-N Fumaric acid Chemical compound OC(=O)\C=C\C(O)=O VZCYOOQTPOCHFL-OWOJBTEDSA-N 0.000 description 2
- 206010020565 Hyperaemia Diseases 0.000 description 2
- 229930194542 Keto Natural products 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- KAGFURAUZIWOGM-UHFFFAOYSA-N N1(C(CCC1)=O)C(=O)O.N1C=CC=C1 Chemical compound N1(C(CCC1)=O)C(=O)O.N1C=CC=C1 KAGFURAUZIWOGM-UHFFFAOYSA-N 0.000 description 2
- KAESVJOAVNADME-UHFFFAOYSA-N Pyrrole Chemical compound C=1C=CNC=1 KAESVJOAVNADME-UHFFFAOYSA-N 0.000 description 2
- 241000700157 Rattus norvegicus Species 0.000 description 2
- 208000025865 Ulcer Diseases 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 230000002155 anti-virotic effect Effects 0.000 description 2
- 239000003443 antiviral agent Substances 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 244000309466 calf Species 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 239000002537 cosmetic Substances 0.000 description 2
- 230000003013 cytotoxicity Effects 0.000 description 2
- 231100000135 cytotoxicity Toxicity 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000013129 endoscopic sinus surgery Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 238000011049 filling Methods 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 210000002216 heart Anatomy 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 125000000468 ketone group Chemical group 0.000 description 2
- 210000003734 kidney Anatomy 0.000 description 2
- 210000004072 lung Anatomy 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 201000009240 nasopharyngitis Diseases 0.000 description 2
- 230000010412 perfusion Effects 0.000 description 2
- 210000003800 pharynx Anatomy 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 150000003235 pyrrolidines Chemical class 0.000 description 2
- 238000003753 real-time PCR Methods 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 230000000392 somatic effect Effects 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- 231100000397 ulcer Toxicity 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- QGZCUOLOTMJILH-UHFFFAOYSA-N 2h-tetrazol-2-ium;bromide Chemical compound [Br-].C1=N[NH+]=NN1 QGZCUOLOTMJILH-UHFFFAOYSA-N 0.000 description 1
- FCKUBAVAZWFDNQ-UHFFFAOYSA-N 5-oxopyrrolidine-2-carboxylic acid zinc Chemical compound [Zn].OC(=O)C1CCC(=O)N1 FCKUBAVAZWFDNQ-UHFFFAOYSA-N 0.000 description 1
- 206010002198 Anaphylactic reaction Diseases 0.000 description 1
- 201000008283 Atrophic Rhinitis Diseases 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 240000004307 Citrus medica Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000008967 Enuresis Diseases 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 229940124091 Keratolytic Drugs 0.000 description 1
- 206010027336 Menstruation delayed Diseases 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- RWAXQWRDVUOOGG-UHFFFAOYSA-N N,2,3-Trimethyl-2-(1-methylethyl)butanamide Chemical compound CNC(=O)C(C)(C(C)C)C(C)C RWAXQWRDVUOOGG-UHFFFAOYSA-N 0.000 description 1
- ZGXNJZTZWUFBES-UHFFFAOYSA-N N1C(CCC1C(=O)O)=O.[K] Chemical compound N1C(CCC1C(=O)O)=O.[K] ZGXNJZTZWUFBES-UHFFFAOYSA-N 0.000 description 1
- 208000000592 Nasal Polyps Diseases 0.000 description 1
- 241000158526 Nasalis Species 0.000 description 1
- 206010030113 Oedema Diseases 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 239000004698 Polyethylene Substances 0.000 description 1
- 238000011530 RNeasy Mini Kit Methods 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 206010039088 Rhinitis atrophic Diseases 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 208000009205 Tinnitus Diseases 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- DPXJVFZANSGRMM-UHFFFAOYSA-N acetic acid;2,3,4,5,6-pentahydroxyhexanal;sodium Chemical compound [Na].CC(O)=O.OCC(O)C(O)C(O)C(O)C=O DPXJVFZANSGRMM-UHFFFAOYSA-N 0.000 description 1
- 229910052783 alkali metal Inorganic materials 0.000 description 1
- 150000003862 amino acid derivatives Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 238000001949 anaesthesia Methods 0.000 description 1
- 230000036783 anaphylactic response Effects 0.000 description 1
- 208000003455 anaphylaxis Diseases 0.000 description 1
- 210000003484 anatomy Anatomy 0.000 description 1
- 230000003466 anti-cipated effect Effects 0.000 description 1
- 210000000702 aorta abdominal Anatomy 0.000 description 1
- 230000004596 appetite loss Effects 0.000 description 1
- 238000013096 assay test Methods 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000019636 bitter flavor Nutrition 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 238000010241 blood sampling Methods 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 235000001465 calcium Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000001768 carboxy methyl cellulose Substances 0.000 description 1
- 239000013553 cell monolayer Substances 0.000 description 1
- 230000007541 cellular toxicity Effects 0.000 description 1
- 201000009151 chronic rhinitis Diseases 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000002270 dispersing agent Substances 0.000 description 1
- 229940059082 douche Drugs 0.000 description 1
- 238000002651 drug therapy Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 230000003628 erosive effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001530 fumaric acid Substances 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000002695 general anesthesia Methods 0.000 description 1
- 235000001727 glucose Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 230000035931 haemagglutination Effects 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 230000001969 hypertrophic effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000012151 immunohistochemical method Methods 0.000 description 1
- 238000011532 immunohistochemical staining Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000001524 infective effect Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 239000007928 intraperitoneal injection Substances 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000001530 keratinolytic effect Effects 0.000 description 1
- 230000031700 light absorption Effects 0.000 description 1
- 235000021266 loss of appetite Nutrition 0.000 description 1
- 208000019017 loss of appetite Diseases 0.000 description 1
- 230000005980 lung dysfunction Effects 0.000 description 1
- 210000003563 lymphoid tissue Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 210000000214 mouth Anatomy 0.000 description 1
- 208000016366 nasal cavity polyp Diseases 0.000 description 1
- 239000007922 nasal spray Substances 0.000 description 1
- 229940097496 nasal spray Drugs 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000036407 pain Effects 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 239000003182 parenteral nutrition solution Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000010603 pastilles Nutrition 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 239000008177 pharmaceutical agent Substances 0.000 description 1
- 230000003285 pharmacodynamic effect Effects 0.000 description 1
- 230000035935 pregnancy Effects 0.000 description 1
- 239000003755 preservative agent Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000013139 quantization Methods 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 239000003507 refrigerant Substances 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 235000019615 sensations Nutrition 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 235000019812 sodium carboxymethyl cellulose Nutrition 0.000 description 1
- 229920001027 sodium carboxymethylcellulose Polymers 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000012109 statistical procedure Methods 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 231100000886 tinnitus Toxicity 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- HFFLGKNGCAIQMO-UHFFFAOYSA-N trichloroacetaldehyde Chemical compound ClC(Cl)(Cl)C=O HFFLGKNGCAIQMO-UHFFFAOYSA-N 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
Description
组名 | ICAM |
正常组 | 1132.50±112.23 |
模型组 | 2451.30±145.12 |
试验组1+病毒 | 2134.60±178.11 |
试验组2+病毒 | 2251.80±132.44 |
试验组3+病毒 | 1543.40±144.03 |
试验组4+病毒 | 1365.60±167.32 |
TNF-ɑ | 4522.30±181.91 |
试验组1+TNF-ɑ | 1212.40±145.34 |
试验组2+TNF-ɑ | 1321.40±121.61 |
试验组3+TNF-ɑ | 4340.20±198.21 |
试验组4+TNF-ɑ | 4410.30±145.41 |
0分 | 1分 | |
畏寒 | 无 | 有 |
自汗 | 无 | 有 |
乏力 | 无 | 有 |
纳呆 | 无 | 有 |
便溏 | 无 | 有 |
夜尿清长 | 无 | 有 |
耳鸣 | 无 | 有 |
组别 | n | 效果显著 | 有效 | 无效 | 总有效率 |
对照组 | 30 | 5 | 20 | 5 | 83.33% |
试验组1 | 50 | 4 | 38 | 8 | 84% |
试验组2 | 50 | 0 | 38 | 12 | 76% |
试验组3 | 50 | 7 | 36 | 7 | 86% |
试验组4 | 50 | 0 | 35 | 15 | 70% |
试验组5 | 50 | 0 | 40 | 10 | 80% |
组别 | N | 第一层次 | 第二层次 | 第三层次 | 第四层次 | 有效率 |
试验1组 | 102 | 31(30.4) | 69(67.6) | 2(2.0) | 0 | 98 |
试验2组 | 102 | 26(25.5) | 72(70.6) | 4(3.9) | 0 | 96.1 |
试验3组 | 102 | 6(5.9) | 72(70.6) | 24(23.5) | 0 | 76.5 |
试验4组 | 102 | 14(13.7) | 70(68.6) | 17(16.7) | 1(1) | 90.2 |
对照组 | 102 | 11(10.8) | 68(66.7) | 21(20.0) | 2(2.5) | 77.4 |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510412461.4A CN104940994B (zh) | 2015-07-14 | 2015-07-14 | 一种人造鼻粘膜及其用途 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510412461.4A CN104940994B (zh) | 2015-07-14 | 2015-07-14 | 一种人造鼻粘膜及其用途 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104940994A CN104940994A (zh) | 2015-09-30 |
CN104940994B true CN104940994B (zh) | 2017-09-19 |
Family
ID=54156406
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510412461.4A Active CN104940994B (zh) | 2015-07-14 | 2015-07-14 | 一种人造鼻粘膜及其用途 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104940994B (zh) |
Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107441560A (zh) * | 2016-05-30 | 2017-12-08 | 任汉学 | 一种多糖生物鼻腔护理喷剂及其制备方法 |
CN107441559A (zh) * | 2016-05-30 | 2017-12-08 | 任汉学 | 一种多糖生物高渗鼻腔护理喷剂及其制备方法 |
CN106727279A (zh) * | 2016-11-29 | 2017-05-31 | 江苏长泰药业有限公司 | 抗过敏和防空气中pm2.5的鼻用组合物及其制备方法和用途 |
CN107007823B (zh) * | 2017-03-21 | 2020-07-14 | 深圳市康思得蛋白质工程有限公司 | 一种用于治疗鼻炎的药物及其制备方法 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
PE20010859A1 (es) * | 1999-10-19 | 2001-09-02 | Procter & Gamble | Productos de papel tisu que contienen agentes antivirales que son suaves a la piel |
US7166575B2 (en) * | 2002-12-17 | 2007-01-23 | Nastech Pharmaceutical Company Inc. | Compositions and methods for enhanced mucosal delivery of peptide YY and methods for treating and preventing obesity |
US7811606B2 (en) * | 2003-04-16 | 2010-10-12 | Dey, L.P. | Nasal pharmaceutical formulations and methods of using the same |
US9808471B2 (en) * | 2003-04-16 | 2017-11-07 | Mylan Specialty Lp | Nasal pharmaceutical formulations and methods of using the same |
AU2005251786A1 (en) * | 2004-06-07 | 2005-12-22 | Nastech Pharmaceutical Company Inc. | Intranasal formulations of interferon beta free of stabilizers that are proteins or polypeptides |
CN101396431B (zh) * | 2007-09-30 | 2012-02-22 | 童玉新 | 鼻通宁凝胶及其制备方法 |
CN102048784A (zh) * | 2009-11-10 | 2011-05-11 | 董根荣 | 治疗鼻炎的喷雾剂及其制备方法 |
CN104667256B (zh) * | 2015-03-18 | 2017-05-03 | 江苏威克斯医疗科技有限公司 | 一种鼻腔粘膜纤毛护理冲洗液及其用途 |
-
2015
- 2015-07-14 CN CN201510412461.4A patent/CN104940994B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN104940994A (zh) | 2015-09-30 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102079794B (zh) | 用于医药装置和药物制剂中的含有木葡聚糖的粘膜粘附性制剂 | |
CN104940994B (zh) | 一种人造鼻粘膜及其用途 | |
CN106668832B (zh) | 一种多肽在制备治疗肠道病毒感染药物的应用 | |
JP4012665B2 (ja) | クリル酵素の新規な薬学的用法 | |
CN104127749A (zh) | 一种治疗有机磷农药中毒的中药及制备方法 | |
CN108743776A (zh) | 一种用于鼻部疾病防治的冲洗粉 | |
CN107899006A (zh) | 冲洗鼻腔的药物组合物及其制备方法 | |
CN105106318B (zh) | 一种治疗干眼症的中药组合物及其制备方法 | |
WO2004103399A1 (fr) | Utilisation d'ulinastatine pour traiter le syndrome respiratoire aigu et composition pharmaceutique contenant ulinastatine | |
US9283258B2 (en) | Herpes treatment | |
US20120021060A1 (en) | Honey Compositions and Uses Thereof | |
EP3570815B1 (en) | Composition for ophthalmic use | |
CN106075402A (zh) | 一种治疗妇科炎症的药物及其制备方法 | |
CN100423731C (zh) | 一种明目洗眼浴眼液及其制备方法 | |
CN112472669A (zh) | 一种基于红辣椒提取物和肝素预防流感的鼻腔喷雾剂及其制备方法 | |
CN108815403A (zh) | 一种外用给药的治疗皮肤组织受损的竹栀五黄液、用途 | |
RU2726001C1 (ru) | Биологически активная композиция Акваплант | |
CN107583050A (zh) | 一种用于治疗宫颈炎的药物组合物及用途 | |
CN103948709B (zh) | 一种维吾尔药热合美康宫栓剂及其制备方法 | |
CN107095977A (zh) | 一种具有祛火排毒功效的药剂及其药物配方和制备方法 | |
CN1227107A (zh) | 一种外用止血散 | |
CN108245506B (zh) | 牛蒡子苷元在制备角膜溃疡治疗药物中的用途 | |
JP6232631B2 (ja) | 生体皮膜剤 | |
Passali et al. | Phitoterapy in rhinosinusitis treatment | |
US20150190455A1 (en) | Herpes Treatment |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Han Wei Inventor after: Jiang Wenfeng Inventor after: Shi Min Inventor after: Yu Yaping Inventor before: Yu Yaping Inventor before: Shi Min |
|
TR01 | Transfer of patent right |
Effective date of registration: 20240605 Address after: 411100 West side of the first floor, Building 9, Innovation and Entrepreneurship Center, No. 31 Dongfeng Road, Economic Development Zone, Heping Street, Xiangtan City, Hunan Province Patentee after: Hunan Weike Medical Technology Co.,Ltd. Country or region after: China Address before: On the west side of the fourth floor, No. 65, Building G58, east of Lujia Road, west of Tai Road, China Pharmaceutical City, Taizhou City, Jiangsu Province, 225300 Patentee before: JIANGSU WEIKESI MEDICAL SCIENCE & TECHNOLOGY CO.,LTD. Country or region before: China |