CN104920791A - A fermented fish meal with high digestibility rate and a preparation method thereof - Google Patents
A fermented fish meal with high digestibility rate and a preparation method thereof Download PDFInfo
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Abstract
The present invention relates to the technical field of fish meal and particularly relates to a fermented fish meal with high digestibility rate and a preparation method thereof. The preparation method comprises the following steps: A, strain preparation: microbial strains are subjected to a first seed culture, a second seed culture and a liquid fermentation to produce a fermented bacteria liquid; and B, inoculated fermentation: the fish meal is inoculated to the above fermented bacteria liquid at a weight ratio of 100: 30-50, the inoculated fish meal is put into a packaging bag attached with a pressure adjustable silica gel film, the sealed packaging bag is stored at room temperature to conduct a fermentation and the fermented fish meal with high digestibility rate is obtained when the pH drops to 3.0-4.0. The fermented fish meal with high digestibility rate is prepared by conducting the microbial fermentation of the fish meal, contains a lot of beneficial microbes, can improve the digestibility rate of proteins; and due to the metabolites produced during the fermentation and the degradation function of the fermentation substrate, the fermented fish meal with high digestibility rate has very complicated ingredients, and the quality of the fish meal protein is improved.
Description
Technical field
The present invention relates to fish meal technical field, be specifically related to a kind of high digestibility fermentation fish meal and preparation method thereof.
Background technology
Fish meal is indispensable high-quality protein source in animal feed, it has the multiple advantages such as biological value is high, energy value is high, calcic phosphorus is high, micronutrient levels is high, digestibility is high, so fish meal becomes the main source of protein sources, calcium phosphorus and energy matter in aquatic feeds and fowl poultry kind feed for a long time in raising application.But the digestive utilization ratio of existing fish meal is relatively low, therefore, the task of top priority that some new and high technologies can improving vegetable protein utilization rate and conversion ratio become practitioner is sought and develops.
Summary of the invention
In order to overcome the shortcoming and defect existed in prior art, the object of the present invention is to provide a kind of preparation method of high digestibility fermentation fish meal, this preparation method is by undergoing microbial fermentation fish meal, obtained high digestibility fermentation fish meal, it contains a large amount of beneficial microbes, can improve the digestibility of protein; And the metabolite of sweat generation and the degradation to fermentation substrate, make the composition of high digestibility fermentation fish meal very complicated, improve the quality of fish meal protein.
Another object of the present invention is to provide a kind of high digestibility fermentation fish meal, this high digestibility fermentation fish meal greatly reduces the abnormal fermentation of feed in large intestine, thus reduces or eliminate trophism diarrhea; Significantly improve the absorption rate of amino acid and mineral matter element; Significantly improve insulin level in animal blood, promote lymphocytosis, improve animal immune level; The breeding of harmful bacteria in enteron aisle can be suppressed, keep the micro-ecological environment of intestinal health, improve animal small intestine function, even make high digestibility fermentation fish meal polypeptide portion substitute antibiotics become possibility; The digestibility of feed can be improved.
Object of the present invention is achieved through the following technical solutions: a kind of preparation method of high digestibility fermentation fish meal, comprises the steps:
Prepared by A, bacterial classification: cultivated through first order seed by microorganism fungus kind, secondary seed cultivates and liquid fermentation and culture obtains zymocyte liquid;
A1, first order seed are cultivated: be inoculated in primary-seed medium by microorganism fungus kind inclined-plane in the ratio of ring/80-120mL, and fermentation obtains primary seed solution;
A2, secondary seed are cultivated: be inoculated in secondary seed medium by primary seed solution by weight 5-15:100, and fermentation obtains secondary seed solution;
A3, liquid fermentation and culture: be inoculated in liquid fermentation medium by secondary seed solution by weight 5-15:100, fermentation obtains zymocyte liquid;
B, inoculation fermentation: the zymocyte liquid that fish meal is obtained by weight 100:30-50 inoculation step A, postvaccinal fish meal is loaded in packaging bag, in packaging bag additional one can the pellosil of adjustable pressure, fermentation is stored at normal temperatures after sealing, until pH is down to 3.0-4.0, obtained high digestibility fermentation fish meal.
Preparation method of the present invention is by undergoing microbial fermentation fish meal, and obtain high digestibility fermentation fish meal, it contains a large amount of beneficial microbes, can improve the digestibility of protein; And the metabolite of sweat generation and the degradation to fermentation substrate, make the composition of high digestibility fermentation fish meal very complicated, improve the quality of fish meal protein.
The high digestibility fermentation fish meal of tradition is all first make product, then packs.Pellosil packaged type anaerobic solid-state fermentation technique is that packaging bag is designed to small-sized fermentation tank, adopts and first packs after fermentation technique.Pellosil bascule creatively solves the technical barrier such as gas control and living contaminants in fermentation production process.During the fermentation, microbes produces the gases such as carbon dioxide, makes the air pressure in bag be greater than extraneous normal pressure.When bag internal gas pressure reaches a certain critical value, gas is discharged to the external world by this pellosil.But the gas in the external world has no chance to enter fermentation system all the time, thus also just eliminate extraneous miscellaneous bacteria interference.
The present invention is by adopting respiratory membrane packaged type anaerobic solid-state fermentation technique, and sweat does not need to carry out temperature adjustment, not influenced by ambient temperature yet.Time required for fermenting-ripening is with environment temperature and the change of material composition, but the shelf-life is not by time restriction, as long as packaging bag is intact, the shelf-life can reach 3 years.
Preparation method of the present invention is simple to operate, if to produce 30 tons of calculating daily, equipment investment is no more than 200,000 yuan, is particularly suitable for middle-size and small-size use.
Preferably, in described steps A 1, microorganism fungus kind comprises the raw material of following weight portion: S. cervisiae 20-30 part, Lactobacillus casei 15-25 part, bacillus subtilis 10-20 part, actinomyces 5-15 part and photosynthetic bacteria 8-12 part.
Microorganism fungus kind of the present invention is by adopting S. cervisiae, it can utilize the carbon source in culture medium, photosynthetic bacteria is synthesized amino acid, carbohydrate and other organic substance produce fermenting power, provide important subsistence support for the matrix (food) promoted required for other effective microbe (as Lactobacillus casei, actinomyces) propagation; Saccharomycete creates anaerobic environment as the growth and breeding existing for Lactobacillus casei of aerobic bacteria, and in addition, the single cell protein that saccharomycete produces is the indispensable nutrient of animal.
Microorganism fungus kind of the present invention is by adopting Lactobacillus casei, and it forms lactic acid by the carbohydrate absorbing photosynthetic bacteria, S. cervisiae produces, and lactic acid has very strong sterilizing ability, effectively can suppress the movable and organic sharply corrupt decomposition of harmful microbe.Lactobacillus casei amount reproduction produces lactic acid, and reduce pH value, this just makes high digestibility fermentation fish meal finished product have strong sour fragrance, thus improves palatability, provides the feed intake of animal.
Microorganism fungus kind of the present invention is by adopting bacillus subtilis, its available protein, multiple sugar and starch, the subtilin produced in its growth course, polymyxins, nystatin, gramicidins isoreactivity material, these active materials have obvious inhibitory action to pathogenic bacteria; Bacillus can kill the harmful microorganisms such as Escherichia coli, salmonella and staphylococcus aureus efficiently, but does not affect the growth metabolism of Lactobacillus casei and S. cervisiae; Bacillus subtilis creates anaerobic environment as the growth and breeding existing for lactic acid bacteria of aerobic bacteria.
Microorganism fungus kind of the present invention is by adopting actinomyces, and it obtains amino acid, nitrogen etc. as matrix from photosynthetic bacteria, produces various antibiotics, vitamin and enzyme, directly can suppress pathogen.Actinomyces obtain the matrix required for harmful mould and bacterial multiplication in advance, thus suppress their propagation, and create the living environment of other beneficial microbe propagation.The material decomposed difficulty after actinomyces and photosynthetic bacteria mix, as lignin, cellulose, chitin etc. have degradation, and is easily absorbed by animal, strengthens animal to the resistance of various disease and immunity.
Microorganism fungus kind of the present invention is by adopting photosynthetic bacteria, and it belongs to autotrophy microorganism, and thalline itself contains the protein of more than 60%, and is rich in multivitamin, also containing Co-Q10, antiviral substance and somatomedin; It take light and heat as the energy, is matrix by organic matter, carbon dioxide, nitrogen etc., and synthesis carbohydrate, amino acids, vitamins, nitrogen compound, antiviral substance and physiological activator etc. are the main powers promoting growth of animal.
Microorganism fungus kind of the present invention is by adopting S. cervisiae, Lactobacillus casei, bacillus subtilis, actinomyces and photosynthetic bacteria composite, and strict its weight proportion of control, combine the advantage of anaerobic bacteria and aerobic bacteria fermentation, raw material does not need sterilization just can be directly used in inoculated and cultured; And each quasi-microorganism plays an important role all separately, central role is that photosynthetic bacteria and Lactobacillus casei are taken as the leading factor, its synthesis capability supports the activity of other microorganisms, the material simultaneously also utilizing other microorganisms to produce, form the relation of coexistence and co-prosperity, ensure that microorganism fungus kind of the present invention is in stable condition, multiple functional, give play to composite optimum efficiency.
Microorganism fungus kind of the present invention can produce polyphenoils, remove oxidation material, eliminate corrupt, suppress pathogen, form the good environment being suitable for growth of animal, simultaneously, it also produces a large amount of is easily the benefit materials of animal absorption, as amino acid, organic acid, Polysaccharides, various vitamin, various biochemical enzymes, somatomedin, antibiotic and antiviral substance etc., improve the immunologic function of animal, promotion health grows.
Microorganism fungus kind of the present invention itself does not produce poisonous and harmful substance, and can not endanger the intrinsic ecological balance of environment.Microorganism fungus kind of the present invention itself has fine growth metabolism vigor, and can effectively degrade large molecule and ANFs, synthesize the small-molecule substances such as little peptide and organic acid; And can protect and strengthen animal body microflora balance, promote animal health.
Preferably, in described steps A 1, the preparation method of primary-seed medium comprises the steps: that getting peptone 12-16g 500mL hot water dissolves, add beef extract 11-15g respectively, yeast extract 11-15g, glucose 6-8g, malt extract medium 2-4g, sodium acetate 2-4g, ammonium citrate 0.5-1.5g, potassium dihydrogen phosphate 0.5-1.5g, magnesium sulfate 0.3-0.5g and tween 0.4-0.8g, supplementing water is to 1L, adjust pH is to 6.0-7.0, packing is carried out after dissolving, the bottled liquid 500-700mL of every 1000mL triangle, at 110-130 DEG C of temperature, autoclaving 20-40min is carried out under 0.1-0.2MPa pressure, be cooled to 20-30 DEG C, obtain primary-seed medium.
Preferably, in described steps A 1, the condition that first order seed is cultivated: fermentation temperature is 28-32 DEG C, and shaking speed is 150-250r/min, sealing and fermenting 3-5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0-4.0.
Preferably, in described steps A 2, the preparation method of secondary seed medium comprises the steps: to get whole milk powder 12-16g 500mL hot water dissolving, add beef extract 11-15g respectively, yeast extract 11-15g, malt extract 4-8g, glucose 6-8g, cane molasses 8-12g, sodium acetate 2-4g, ammonium citrate 0.5-1.5g, potassium dihydrogen phosphate 0.5-1.5g, magnesium sulfate 0.3-0.5g and tween 0.4-0.8g, supplementing water is to 1L, adjust pH is to 6.0-7.0, packing is carried out after dissolving, the bottled liquid 500-700mL of every 1000mL triangle, at 110-130 DEG C of temperature, autoclaving 20-40min is carried out under 0.1-0.2MPa pressure, be cooled to 20-30 DEG C, obtain secondary seed medium.
Preferably, in described steps A 2, the condition that secondary seed is cultivated: fermentation temperature is 28-32 DEG C, and shaking speed is 150-250r/min, sealing and fermenting 5-7d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0-4.0.
Preferably, in described steps A 3, the preparation method of liquid fermentation medium comprises the steps: to get peptone 3-5g 500mL hot water dissolving, add glucose 10-20g respectively, beef extract 3-5g, bean cake powder 3-5g, cornstarch 2-4g, phytase 1-3g, potassium dihydrogen phosphate 0.5-1.5g, magnesium phosphate 0.3-0.5g, sodium chloride 0.5-1.5g and tween 0.4-0.8g, supplementing water is to 1L, adjust pH is to 6.0-7.0, packing is carried out after dissolving, the bottled liquid 500-700mL of every 1000mL triangle, at 110-130 DEG C of temperature, autoclaving 20-40min is carried out under 0.1-0.2MPa pressure, be cooled to 20-30 DEG C, obtain secondary seed medium.
Preferably, in described steps A 3, the condition of liquid fermentation and culture: fermentation temperature is 28-32 DEG C, shaking speed is 150-250r/min, sealing and fermenting 3-5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0-4.0.
Preferably, in described step B, in fish meal, be added with the fulvic acid accounting for fish meal weight 1%-2%.
The present invention, by adopting fulvic acid, and controls the 1%-2% that its consumption is fish meal weight, has hemostasis, anti-inflammatory, convergence, absorption, antiallergy, shortly to secrete, remove necrosis and promote granulation, adjusts gastrointestinal function, the effects such as raising immunity of organisms.Fulvic acid is nutriment, is again class growth hormone, to raising feed nutrition, promotes growth of animal, increases the resistance against diseases of body and resistance to oxidation, and treatment viral infectious aspect has and acts on very significantly.
Another object of the present invention is achieved through the following technical solutions: a kind of high digestibility fermentation fish meal, and described high digestibility fermentation fish meal obtains according to preparation method described above.
High digestibility fermentation fish meal of the present invention greatly reduces the abnormal fermentation of feed in large intestine, thus reduces or eliminate trophism diarrhea; Significantly improve the absorption rate of amino acid and mineral matter element; Significantly improve insulin level in animal blood, promote lymphocytosis, improve animal immune level; The breeding of harmful bacteria in enteron aisle can be suppressed, keep the micro-ecological environment of intestinal health, improve animal small intestine function, even make high digestibility fermentation fish meal polypeptide portion substitute antibiotics become possibility; The digestibility of feed can be improved.
Beneficial effect of the present invention is: preparation method of the present invention is by undergoing microbial fermentation fish meal, and obtain high digestibility fermentation fish meal, it contains a large amount of beneficial microbes, can improve the digestibility of protein; And the metabolite of sweat generation and the degradation to fermentation substrate, make the composition of high digestibility fermentation fish meal very complicated, improve the quality of fish meal protein.
High digestibility fermentation fish meal protein digestibility of the present invention, up to more than 98%, greatly reduces the abnormal fermentation of feed in large intestine, thus reduces or eliminate trophism diarrhea; Polypeptide content, more than 70%, significantly improves the absorption rate of amino acid and mineral matter element; There is bioactive little Toplink insulin level in animal blood is provided significantly, promote lymphocytosis, improve animal immune level; Probio and lactic acid, can suppress the breeding of harmful bacteria in enteron aisle, keeps the micro-ecological environment of intestinal health, improve animal small intestine function, even make high digestibility fermentation fish meal polypeptide portion substitute antibiotics become possibility; Microbial metabolic products, as digestive ferment, can improve the digestibility of feed.The UGF, vitamin etc. to the direct trophism of animal is also had in microbe metabolite.On the other hand, the excellent substance characteristics such as the solubility of fish meal polypeptide products is high, viscosity is low, anti-gel formative is good, the anti-oxidant action that caused by stickiness component (as follows the wheat such as powder, wheat bran class) in daily ration is disappeared, such that high digestibility fermentation fish meal polypeptide portion substitutes complex enzyme for feed, Tiny ecosystem becomes possibility.
High digestibility fermentation fish meal of the present invention, by adopting zymotechnique, the harmful microorganism such as kill bacteria, fungi, eliminates the characteristic odor of fish meal product, and degraded fish meal protein is the product such as small molecular protein, little peptide.High digestibility fermentation fish meal of the present invention is as a kind of animal protein raw material of high-quality, and show good value, the addition in animal and fowl fodder and aquatic feeds can arrive 3%-10%, shows better growth-promoting effect.
Detailed description of the invention
For the ease of the understanding of those skilled in the art, below in conjunction with embodiment, the present invention is further illustrated, and the content that embodiment is mentioned not is limitation of the invention.
Embodiment 1
A preparation method for high digestibility fermentation fish meal, comprises the steps:
Prepared by A, bacterial classification: cultivated through first order seed by microorganism fungus kind, secondary seed cultivates and liquid fermentation and culture obtains zymocyte liquid;
A1, first order seed are cultivated: be inoculated in primary-seed medium by microorganism fungus kind inclined-plane in the ratio of ring/80mL, and fermentation obtains primary seed solution;
A2, secondary seed are cultivated: be inoculated in secondary seed medium by primary seed solution by weight 5:100, and fermentation obtains secondary seed solution;
A3, liquid fermentation and culture: be inoculated in liquid fermentation medium by secondary seed solution by weight 5:100, fermentation obtains zymocyte liquid;
B, inoculation fermentation: the zymocyte liquid that fish meal is obtained by weight 100:30 inoculation step A, postvaccinal fish meal is loaded in packaging bag, in packaging bag additional one can the pellosil of adjustable pressure, fermentation is stored at normal temperatures after sealing, until pH is down to 3.0, obtained high digestibility fermentation fish meal.
In described steps A 1, microorganism fungus kind comprises the raw material of following weight portion: S. cervisiae 20 parts, Lactobacillus casei 15 parts, bacillus subtilis 10 parts, 5 parts, actinomyces and photosynthetic bacteria 8 parts.
In described steps A 1, the preparation method of primary-seed medium comprises the steps: that getting peptone 12g 500mL hot water dissolves, add beef extract 11g, yeast extract 11g, glucose 6g, malt extract medium 2g, sodium acetate 2g, ammonium citrate 0.5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.3g and tween 0.4g respectively, supplementing water is to 1L, adjust pH to 6.0, packing is carried out after dissolving, the bottled liquid 500mL of every 1000mL triangle, autoclaving 20min is carried out at 110 DEG C of temperature, under 0.1MPa pressure, be cooled to 20 DEG C, obtain primary-seed medium.
In described steps A 1, the condition that first order seed is cultivated: fermentation temperature is 28 DEG C, and shaking speed is 150r/min, sealing and fermenting 3d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0.
In described steps A 2, the preparation method of secondary seed medium comprises the steps: to get whole milk powder 12g 500mL hot water dissolving, add beef extract 11g respectively, yeast extract 11g, malt extract 4g, glucose 6g, cane molasses 8g, sodium acetate 2g, ammonium citrate 0.5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.3g and tween 0.4g, supplementing water is to 1L, adjust pH to 6.0, packing is carried out after dissolving, the bottled liquid 500mL of every 1000mL triangle, at 110 DEG C of temperature, autoclaving 20min is carried out under 0.1MPa pressure, be cooled to 20 DEG C, obtain secondary seed medium.
In described steps A 2, the condition that secondary seed is cultivated: fermentation temperature is 28 DEG C, and shaking speed is 150r/min, sealing and fermenting 5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0.
In described steps A 3, the preparation method of liquid fermentation medium comprises the steps: to get peptone 3g 500mL hot water dissolving, add glucose 10g, beef extract 3g, bean cake powder 3g, cornstarch 2g, phytase 1g, potassium dihydrogen phosphate 0.5g, magnesium phosphate 0.3g, sodium chloride 0.5g and tween 0.4g respectively, supplementing water is to 1L, adjust pH to 6.0, packing is carried out after dissolving, the bottled liquid 500mL of every 1000mL triangle, autoclaving 20min is carried out at 110 DEG C of temperature, under 0.1MPa pressure, be cooled to 20 DEG C, obtain secondary seed medium.
In described steps A 3, the condition of liquid fermentation and culture: fermentation temperature is 28 DEG C, shaking speed is 150r/min, sealing and fermenting 3d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0.
In described step B, in fish meal, be added with the fulvic acid accounting for fish meal weight 1%.
A kind of high digestibility fermentation fish meal, described high digestibility fermentation fish meal obtains according to preparation method described above.
Embodiment 2
A preparation method for high digestibility fermentation fish meal, comprises the steps:
Prepared by A, bacterial classification: cultivated through first order seed by microorganism fungus kind, secondary seed cultivates and liquid fermentation and culture obtains zymocyte liquid;
A1, first order seed are cultivated: be inoculated in primary-seed medium by microorganism fungus kind inclined-plane in the ratio of ring/90mL, and fermentation obtains primary seed solution;
A2, secondary seed are cultivated: be inoculated in secondary seed medium by primary seed solution by weight 7:100, and fermentation obtains secondary seed solution;
A3, liquid fermentation and culture: be inoculated in liquid fermentation medium by secondary seed solution by weight 7:100, fermentation obtains zymocyte liquid;
B, inoculation fermentation: the zymocyte liquid that fish meal is obtained by weight 100:35 inoculation step A, postvaccinal fish meal is loaded in packaging bag, in packaging bag additional one can the pellosil of adjustable pressure, store fermentation at normal temperatures after sealing, until pH is down to 3, obtained high digestibility fermentation fish meal.
In described steps A 1, microorganism fungus kind comprises the raw material of following weight portion: S. cervisiae 22 parts, Lactobacillus casei 17 parts, bacillus subtilis 12 parts, 7 parts, actinomyces and photosynthetic bacteria 9 parts.
In described steps A 1, the preparation method of primary-seed medium comprises the steps: that getting peptone 13g 500mL hot water dissolves, add beef extract 12g, yeast extract 12g, glucose 6g, malt extract medium 2g, sodium acetate 2g, ammonium citrate 0.7g, potassium dihydrogen phosphate 0.7g, magnesium sulfate 0.35g and tween 0.5g respectively, supplementing water is to 1L, adjust pH to 6.0, packing is carried out after dissolving, the bottled liquid 550mL of every 1000mL triangle, autoclaving 25min is carried out at 115 DEG C of temperature, under 0.12MPa pressure, be cooled to 22 DEG C, obtain primary-seed medium.
In described steps A 1, the condition that first order seed is cultivated: fermentation temperature is 29 DEG C, and shaking speed is 175r/min, sealing and fermenting 3.5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0.
In described steps A 2, the preparation method of secondary seed medium comprises the steps: to get whole milk powder 13g 500mL hot water dissolving, add beef extract 12g respectively, yeast extract 12g, malt extract 5g, glucose 6.5g, cane molasses 9g, sodium acetate 2.5g, ammonium citrate 0.7g, potassium dihydrogen phosphate 0.7g, magnesium sulfate 0.35g and tween 0.5g, supplementing water is to 1L, adjust pH to 6.0, packing is carried out after dissolving, the bottled liquid 550mL of every 1000mL triangle, at 115 DEG C of temperature, autoclaving 25min is carried out under 0.15MPa pressure, be cooled to 22 DEG C, obtain secondary seed medium.
In described steps A 2, the condition that secondary seed is cultivated: fermentation temperature is 29 DEG C, and shaking speed is 175r/min, sealing and fermenting 5.5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0.
In described steps A 3, the preparation method of liquid fermentation medium comprises the steps: to get peptone 3.5g 500mL hot water dissolving, add glucose 12g, beef extract 3.5g, bean cake powder 3.5g, cornstarch 2.5g, phytase 1.5g, potassium dihydrogen phosphate 0.7g, magnesium phosphate 0.35g, sodium chloride 0.7g and tween 0.5g respectively, supplementing water is to 1L, adjust pH to 6.0, packing is carried out after dissolving, the bottled liquid 550mL of every 1000mL triangle, autoclaving 25min is carried out at 115 DEG C of temperature, under 0.12MPa pressure, be cooled to 22 DEG C, obtain secondary seed medium.
In described steps A 3, the condition of liquid fermentation and culture: fermentation temperature is 29 DEG C, shaking speed is 175r/min, sealing and fermenting 3.5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0.
In described step B, in fish meal, be added with the fulvic acid accounting for fish meal weight 1.2%.
A kind of high digestibility fermentation fish meal, described high digestibility fermentation fish meal obtains according to preparation method described above.
Embodiment 3
A preparation method for high digestibility fermentation fish meal, comprises the steps:
Prepared by A, bacterial classification: cultivated through first order seed by microorganism fungus kind, secondary seed cultivates and liquid fermentation and culture obtains zymocyte liquid;
A1, first order seed are cultivated: be inoculated in primary-seed medium by microorganism fungus kind inclined-plane in the ratio of ring/100mL, and fermentation obtains primary seed solution;
A2, secondary seed are cultivated: be inoculated in secondary seed medium by primary seed solution by weight 10:100, and fermentation obtains secondary seed solution;
A3, liquid fermentation and culture: be inoculated in liquid fermentation medium by secondary seed solution by weight 10:100, fermentation obtains zymocyte liquid;
B, inoculation fermentation: the zymocyte liquid that fish meal is obtained by weight 100:40 inoculation step A, postvaccinal fish meal is loaded in packaging bag, in packaging bag additional one can the pellosil of adjustable pressure, fermentation is stored at normal temperatures after sealing, until pH is down to 3.0, obtained high digestibility fermentation fish meal.
In described steps A 1, microorganism fungus kind comprises the raw material of following weight portion: S. cervisiae 25 parts, Lactobacillus casei 20 parts, bacillus subtilis 15 parts, 10 parts, actinomyces and photosynthetic bacteria 10 parts.
In described steps A 1, the preparation method of primary-seed medium comprises the steps: that getting peptone 14g 500mL hot water dissolves, add beef extract 13g, yeast extract 13g, glucose 7g, malt extract medium 3g, sodium acetate 3g, ammonium citrate 1g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.4g and tween 0.6g respectively, supplementing water is to 1L, adjust pH to 6.0, packing is carried out after dissolving, the bottled liquid 600mL of every 1000mL triangle, autoclaving 30min is carried out at 120 DEG C of temperature, under 0.15MPa pressure, be cooled to 25 DEG C, obtain primary-seed medium.
In described steps A 1, the condition that first order seed is cultivated: fermentation temperature is 30 DEG C, and shaking speed is 200r/min, sealing and fermenting 4d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0.
In described steps A 2, the preparation method of secondary seed medium comprises the steps: to get whole milk powder 14g 500mL hot water dissolving, add beef extract 13g respectively, yeast extract 13g, malt extract 6g, glucose 7g, cane molasses 10g, sodium acetate 3g, ammonium citrate 1g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.4g and tween 0.6g, supplementing water is to 1L, adjust pH to 6.0, packing is carried out after dissolving, the bottled liquid 600mL of every 1000mL triangle, at 120 DEG C of temperature, autoclaving 30min is carried out under 0.15MPa pressure, be cooled to 25 DEG C, obtain secondary seed medium.
In described steps A 2, the condition that secondary seed is cultivated: fermentation temperature is 30 DEG C, and shaking speed is 200r/min, sealing and fermenting 6d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0.
In described steps A 3, the preparation method of liquid fermentation medium comprises the steps: to get peptone 4g 500mL hot water dissolving, add glucose 15g, beef extract 4g, bean cake powder 4g, cornstarch 3g, phytase 2g, potassium dihydrogen phosphate 1g, magnesium phosphate 0.4g, sodium chloride 1g and tween 0.6g respectively, supplementing water is to 1L, adjust pH to 6.0, packing is carried out after dissolving, the bottled liquid 600mL of every 1000mL triangle, autoclaving 30min is carried out at 120 DEG C of temperature, under 0.15MPa pressure, be cooled to 25 DEG C, obtain secondary seed medium.
In described steps A 3, the condition of liquid fermentation and culture: fermentation temperature is 30 DEG C, shaking speed is 200r/min, sealing and fermenting 4d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0.
In described step B, in fish meal, be added with the fulvic acid accounting for fish meal weight 1.5%.
A kind of high digestibility fermentation fish meal, described high digestibility fermentation fish meal obtains according to preparation method described above.
Embodiment 4
A preparation method for high digestibility fermentation fish meal, comprises the steps:
Prepared by A, bacterial classification: cultivated through first order seed by microorganism fungus kind, secondary seed cultivates and liquid fermentation and culture obtains zymocyte liquid;
A1, first order seed are cultivated: be inoculated in primary-seed medium by microorganism fungus kind inclined-plane in the ratio of ring/110mL, and fermentation obtains primary seed solution;
A2, secondary seed are cultivated: be inoculated in secondary seed medium by primary seed solution by weight 12:100, and fermentation obtains secondary seed solution;
A3, liquid fermentation and culture: be inoculated in liquid fermentation medium by secondary seed solution by weight 12:100, fermentation obtains zymocyte liquid;
B, inoculation fermentation: the zymocyte liquid that fish meal is obtained by weight 100:45 inoculation step A, postvaccinal fish meal is loaded in packaging bag, in packaging bag additional one can the pellosil of adjustable pressure, fermentation is stored at normal temperatures after sealing, until pH is down to 4.0, obtained high digestibility fermentation fish meal.
In described steps A 1, microorganism fungus kind comprises the raw material of following weight portion: S. cervisiae 28 parts, Lactobacillus casei 22 parts, bacillus subtilis 18 parts, 12 parts, actinomyces and photosynthetic bacteria 11 parts.
In described steps A 1, the preparation method of primary-seed medium comprises the steps: that getting peptone 15g 500mL hot water dissolves, add beef extract 14g respectively, yeast extract 14g, glucose 7g, malt extract medium 3.5g, sodium acetate 3.5g, ammonium citrate 1.2g, potassium dihydrogen phosphate 1.2g, magnesium sulfate 0.45g and tween 0.7g, supplementing water is to 1L, adjust pH to 7.0, packing is carried out after dissolving, the bottled liquid 650mL of every 1000mL triangle, at 125 DEG C of temperature, autoclaving 35min is carried out under 0.18MPa pressure, be cooled to 28 DEG C, obtain primary-seed medium.
In described steps A 1, the condition that first order seed is cultivated: fermentation temperature is 31 DEG C, and shaking speed is 225r/min, sealing and fermenting 4.5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 4.0.
In described steps A 2, the preparation method of secondary seed medium comprises the steps: to get whole milk powder 15g 500mL hot water dissolving, add beef extract 14g respectively, yeast extract 14g, malt extract 7g, glucose 7g, cane molasses 11g, sodium acetate 3.5g, ammonium citrate 1.2g, potassium dihydrogen phosphate 1.2g, magnesium sulfate 0.45g and tween 0.7g, supplementing water is to 1L, adjust pH to 7.0, packing is carried out after dissolving, the bottled liquid 650mL of every 1000mL triangle, at 125 DEG C of temperature, autoclaving 35min is carried out under 0.18MPa pressure, be cooled to 28 DEG C, obtain secondary seed medium.
In described steps A 2, the condition that secondary seed is cultivated: fermentation temperature is 31 DEG C, and shaking speed is 225r/min, sealing and fermenting 6.5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 4.0.
In described steps A 3, the preparation method of liquid fermentation medium comprises the steps: to get peptone 4.5g 500mL hot water dissolving, add glucose 18g, beef extract 4.5g, bean cake powder 4.5g, cornstarch 3.5g, phytase 2.5g, potassium dihydrogen phosphate 1.2g, magnesium phosphate 0.45g, sodium chloride 1.2g and tween 0.7g respectively, supplementing water is to 1L, adjust pH to 7.0, packing is carried out after dissolving, the bottled liquid 650mL of every 1000mL triangle, autoclaving 35min is carried out at 125 DEG C of temperature, under 0.18MPa pressure, be cooled to 28 DEG C, obtain secondary seed medium.
In described steps A 3, the condition of liquid fermentation and culture: fermentation temperature is 31 DEG C, shaking speed is 225r/min, sealing and fermenting 4.5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 4.0.
In described step B, in fish meal, be added with the fulvic acid accounting for fish meal weight 1.8%.
A kind of high digestibility fermentation fish meal, described high digestibility fermentation fish meal obtains according to preparation method described above.
Embodiment 5
A preparation method for high digestibility fermentation fish meal, comprises the steps:
Prepared by A, bacterial classification: cultivated through first order seed by microorganism fungus kind, secondary seed cultivates and liquid fermentation and culture obtains zymocyte liquid;
A1, first order seed are cultivated: be inoculated in primary-seed medium by microorganism fungus kind inclined-plane in the ratio of ring/120mL, and fermentation obtains primary seed solution;
A2, secondary seed are cultivated: be inoculated in secondary seed medium by primary seed solution by weight 15:100, and fermentation obtains secondary seed solution;
A3, liquid fermentation and culture: be inoculated in liquid fermentation medium by secondary seed solution by weight 15:100, fermentation obtains zymocyte liquid;
B, inoculation fermentation: the zymocyte liquid that fish meal is obtained by weight 100:50 inoculation step A, postvaccinal fish meal is loaded in packaging bag, in packaging bag additional one can the pellosil of adjustable pressure, fermentation is stored at normal temperatures after sealing, until pH is down to 4.0, obtained high digestibility fermentation fish meal.
In described steps A 1, microorganism fungus kind comprises the raw material of following weight portion: S. cervisiae 30 parts, Lactobacillus casei 25 parts, bacillus subtilis 20 parts, 15 parts, actinomyces and photosynthetic bacteria 12 parts.
In described steps A 1, the preparation method of primary-seed medium comprises the steps: that getting peptone 16g 500mL hot water dissolves, add beef extract 15g, yeast extract 15g, glucose 8g, malt extract medium 4g, sodium acetate 4g, ammonium citrate 1.5g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g and tween 0.8g respectively, supplementing water is to 1L, adjust pH to 7.0, packing is carried out after dissolving, the bottled liquid 700mL of every 1000mL triangle, autoclaving 40min is carried out at 130 DEG C of temperature, under 0.2MPa pressure, be cooled to 30 DEG C, obtain primary-seed medium.
In described steps A 1, the condition that first order seed is cultivated: fermentation temperature is 32 DEG C, and shaking speed is 250r/min, sealing and fermenting 5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 4.0.
In described steps A 2, the preparation method of secondary seed medium comprises the steps: to get whole milk powder 16g 500mL hot water dissolving, add beef extract 15g respectively, yeast extract 15g, malt extract 8g, glucose 8g, cane molasses 12g, sodium acetate 4g, ammonium citrate 1.5g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g and tween 0.8g, supplementing water is to 1L, adjust pH to 7.0, packing is carried out after dissolving, the bottled liquid 700mL of every 1000mL triangle, at 130 DEG C of temperature, autoclaving 40min is carried out under 0.2MPa pressure, be cooled to 30 DEG C, obtain secondary seed medium.
In described steps A 2, the condition that secondary seed is cultivated: fermentation temperature is 32 DEG C, and shaking speed is 250r/min, sealing and fermenting 7d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 4.0.
In described steps A 3, the preparation method of liquid fermentation medium comprises the steps: to get peptone 5g 500mL hot water dissolving, add glucose 20g, beef extract 5g, bean cake powder 5g, cornstarch 4g, phytase 3g, potassium dihydrogen phosphate 1.5g, magnesium phosphate 0.5g, sodium chloride 1.5g and tween 0.8g respectively, supplementing water is to 1L, adjust pH to 7.0, packing is carried out after dissolving, the bottled liquid 700mL of every 1000mL triangle, autoclaving 40min is carried out at 130 DEG C of temperature, under 0.2MPa pressure, be cooled to 30 DEG C, obtain secondary seed medium.
In described steps A 3, the condition of liquid fermentation and culture: fermentation temperature is 32 DEG C, shaking speed is 250r/min, sealing and fermenting 5d, measures pH value every day and the gas of release generation, until stop fermentation when pH value is down to 4.0.
In described step B, in fish meal, be added with the fulvic acid accounting for fish meal weight 2%.
A kind of high digestibility fermentation fish meal, described high digestibility fermentation fish meal obtains according to preparation method described above.
Preparation method of the present invention is by undergoing microbial fermentation fish meal, and obtain high digestibility fermentation fish meal, it contains a large amount of beneficial microbes, can improve the digestibility of protein; And the metabolite of sweat generation and the degradation to fermentation substrate, make the composition of high digestibility fermentation fish meal very complicated, improve the quality of fish meal protein.
High digestibility fermentation fish meal protein digestibility of the present invention, up to more than 98%, greatly reduces the abnormal fermentation of feed in large intestine, thus reduces or eliminate trophism diarrhea; Polypeptide content, more than 70%, significantly improves the absorption rate of amino acid and mineral matter element; There is bioactive little Toplink insulin level in animal blood is provided significantly, promote lymphocytosis, improve animal immune level; Probio and lactic acid, can suppress the breeding of harmful bacteria in enteron aisle, keeps the micro-ecological environment of intestinal health, improve animal small intestine function, even make high digestibility fermentation fish meal polypeptide portion substitute antibiotics become possibility; Microbial metabolic products, as digestive ferment, can improve the digestibility of feed.The UGF, vitamin etc. to the direct trophism of animal is also had in microbe metabolite.On the other hand, the excellent substance characteristics such as the solubility of fish meal polypeptide products is high, viscosity is low, anti-gel formative is good, the anti-oxidant action that caused by stickiness component (as follows the wheat such as powder, wheat bran class) in daily ration is disappeared, such that high digestibility fermentation fish meal polypeptide portion substitutes complex enzyme for feed, Tiny ecosystem becomes possibility.
High digestibility fermentation fish meal of the present invention, by adopting zymotechnique, the harmful microorganism such as kill bacteria, fungi, eliminates the characteristic odor of fish meal product, and degraded fish meal protein is the product such as small molecular protein, little peptide.High digestibility fermentation fish meal of the present invention is as a kind of animal protein raw material of high-quality, and show good value, the addition in animal and fowl fodder and aquatic feeds can arrive 3-10%, shows better growth-promoting effect.
Above-described embodiment is the present invention's preferably implementation, and in addition, the present invention can also realize by alternate manner, and any apparent replacement is all within protection scope of the present invention without departing from the inventive concept of the premise.
Claims (10)
1. a preparation method for high digestibility fermentation fish meal, is characterized in that: comprise the steps:
Prepared by A, bacterial classification: cultivated through first order seed by microorganism fungus kind, secondary seed cultivates and liquid fermentation and culture obtains zymocyte liquid;
A1, first order seed are cultivated: be inoculated in primary-seed medium by microorganism fungus kind inclined-plane in the ratio of ring/80-120mL, and fermentation obtains primary seed solution;
A2, secondary seed are cultivated: be inoculated in secondary seed medium by primary seed solution by weight 5-15:100, and fermentation obtains secondary seed solution;
A3, liquid fermentation and culture: be inoculated in liquid fermentation medium by secondary seed solution by weight 5-15:100, fermentation obtains zymocyte liquid;
B, inoculation fermentation: the zymocyte liquid that fish meal is obtained by weight 100:30-50 inoculation step A, postvaccinal fish meal is loaded in packaging bag, in packaging bag additional one can the pellosil of adjustable pressure, fermentation is stored at normal temperatures after sealing, until pH is down to 3.0-4.0, obtained high digestibility fermentation fish meal.
2. the preparation method of a kind of high digestibility fermentation fish meal according to claim 1, it is characterized in that: in described steps A 1, microorganism fungus kind comprises the raw material of following weight portion: S. cervisiae 20-30 part, Lactobacillus casei 15-25 part, bacillus subtilis 10-20 part, actinomyces 5-15 part and photosynthetic bacteria 8-12 part.
3. the preparation method of a kind of high digestibility fermentation fish meal according to claim 1, it is characterized in that: in described steps A 1, the preparation method of primary-seed medium comprises the steps: that getting peptone 12-16g 500mL hot water dissolves, add beef extract 11-15g respectively, yeast extract 11-15g, glucose 6-8g, malt extract medium 2-4g, sodium acetate 2-4g, ammonium citrate 0.5-1.5g, potassium dihydrogen phosphate 0.5-1.5g, magnesium sulfate 0.3-0.5g and tween 0.4-0.8g, supplementing water is to 1L, adjust pH is to 6.0-7.0, packing is carried out after dissolving, the bottled liquid 500-700mL of every 1000mL triangle, at 110-130 DEG C of temperature, autoclaving 20-40min is carried out under 0.1-0.2MPa pressure, be cooled to 20-30 DEG C, obtain primary-seed medium.
4. the preparation method of a kind of high digestibility fermentation fish meal according to claim 1, it is characterized in that: in described steps A 1, the condition that first order seed is cultivated: fermentation temperature is 28-32 DEG C, shaking speed is 150-250r/min, sealing and fermenting 3-5d, measure pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0-4.0.
5. the preparation method of a kind of high digestibility fermentation fish meal according to claim 1, it is characterized in that: in described steps A 2, the preparation method of secondary seed medium comprises the steps: to get whole milk powder 12-16g 500mL hot water dissolving, add beef extract 11-15g respectively, yeast extract 11-15g, malt extract 4-8g, glucose 6-8g, cane molasses 8-12g, sodium acetate 2-4g, ammonium citrate 0.5-1.5g, potassium dihydrogen phosphate 0.5-1.5g, magnesium sulfate 0.3-0.5g and tween 0.4-0.8g, supplementing water is to 1L, adjust pH is to 6.0-7.0, packing is carried out after dissolving, the bottled liquid 500-700mL of every 1000mL triangle, at 110-130 DEG C of temperature, autoclaving 20-40min is carried out under 0.1-0.2MPa pressure, be cooled to 20-30 DEG C, obtain secondary seed medium.
6. the preparation method of a kind of high digestibility fermentation fish meal according to claim 1, it is characterized in that: in described steps A 2, the condition that secondary seed is cultivated: fermentation temperature is 28-32 DEG C, shaking speed is 150-250r/min, sealing and fermenting 5-7d, measure pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0-4.0.
7. the preparation method of a kind of high digestibility fermentation fish meal according to claim 1, it is characterized in that: in described steps A 3, the preparation method of liquid fermentation medium comprises the steps: to get peptone 3-5g 500mL hot water dissolving, add glucose 10-20g respectively, beef extract 3-5g, bean cake powder 3-5g, cornstarch 2-4g, phytase 1-3g, potassium dihydrogen phosphate 0.5-1.5g, magnesium phosphate 0.3-0.5g, sodium chloride 0.5-1.5g and tween 0.4-0.8g, supplementing water is to 1L, adjust pH is to 6.0-7.0, packing is carried out after dissolving, the bottled liquid 500-700mL of every 1000mL triangle, at 110-130 DEG C of temperature, autoclaving 20-40min is carried out under 0.1-0.2MPa pressure, be cooled to 20-30 DEG C, obtain secondary seed medium.
8. the preparation method of a kind of high digestibility fermentation fish meal according to claim 1, it is characterized in that: in described steps A 3, the condition of liquid fermentation and culture: fermentation temperature is 28-32 DEG C, shaking speed is 150-250r/min, sealing and fermenting 3-5d, measure pH value every day and the gas of release generation, until stop fermentation when pH value is down to 3.0-4.0.
9. the preparation method of a kind of high digestibility fermentation fish meal according to claim 1, is characterized in that: in described step B, is added with the fulvic acid accounting for fish meal weight 1%-2% in fish meal.
10. a high digestibility fermentation fish meal, is characterized in that: the preparation method of described high digestibility fermentation fish meal according to any one of claim 1-9 obtains.
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| CN106173213A (en) * | 2016-07-06 | 2016-12-07 | 中国科学院合肥物质科学研究院 | A kind of manufacture method of fish flour |
| CN109123726A (en) * | 2018-10-08 | 2019-01-04 | 何秋宏 | A kind of preparation method of feed fermentation fish meal |
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