CN104920791B - A kind of high digestibility fermentation fish meal and preparation method thereof - Google Patents
A kind of high digestibility fermentation fish meal and preparation method thereof Download PDFInfo
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Abstract
The present invention relates to fish meal technical fields, and in particular to a kind of high digestibility fermentation fish meal and preparation method thereof, which includes the following steps：A, prepared by strain：Zymocyte liquid is made through first order seed culture, secondary seed culture and liquid fermentation and culture in microorganism fungus kind；B, inoculation fermentation：By fish meal by weight 100:The 30 50 above-mentioned zymocyte liquids of inoculation, the fish meal after inoculation is fitted into packaging bag, a pellosil that can adjust air pressure is added in packaging bag, storage fermentation at normal temperatures after sealing, until pH is down to 3.0 4.0, obtained high digestibility fermentation fish meal.The preparation method of the present invention is made high digestibility fermentation fish meal, containing a large amount of beneficial microbe, can improve the digestibility of protein by the way that fish meal is undergone microbial fermentation；And fermentation process generate metabolite and the degradation to fermentation substrate so that the ingredient of high digestibility fermentation fish meal is extremely complex, improves the quality of fish meal protein.
Description
Technical field
The present invention relates to fish meal technical fields, and in particular to a kind of high digestibility fermentation fish meal and preparation method thereof.
Background technology
Fish meal is indispensable high-quality protein source in animal feed, it have in raising is applied biological value it is high,
A variety of advantages such as energy value height, calcic phosphorus height, micronutrient levels height, digestibility height, so fish meal becomes water for a long time
Produce the main source of protein sources, calcium phosphorus and energy matter in feed and fowl poultry kind feed.But the digestive utilization ratio phase of existing fish meal
To relatively low, therefore, seek and develop some and can improve vegetable protein utilization rate and the new and high technology of conversion ratio to become
The task of top priority of practitioner.
Invention content
In order to overcome shortcoming and defect in the prior art, the purpose of the present invention is to provide a kind of high digestibilities to send out
The preparation method of ferment fish meal, the preparation method are made high digestibility fermentation fish meal, contain by the way that fish meal is undergone microbial fermentation
There is a large amount of beneficial microbe, the digestibility of protein can be improved；And fermentation process generate metabolite and to fermentation
The degradation of substrate so that the ingredient of high digestibility fermentation fish meal is extremely complex, improves the quality of fish meal protein.
Another object of the present invention is to provide a kind of high digestibility fermentation fish meal, the high digestibility fermentation fish meal is great
Reduce abnormal fermentation of the feed in large intestine, so as to reduce or eliminate trophism diarrhea；Significantly improve amino acid and mineral
The absorption rate of prime element；Insulin level in animal blood is significantly improved, promotes lymphocytosis, improves animal immune
It is horizontal；It can inhibit the breeding of harmful bacteria in enteron aisle, keep the micro-ecological environment of intestinal health, improve animal small intestine function, even
Make it possible high digestibility fermentation fish meal polypeptide portion substitute antibiotics；The digestibility of feed can be improved.
The purpose of the present invention is achieved through the following technical solutions：A kind of preparation method of high digestibility fermentation fish meal, including
Following steps：
A, prepared by strain：Microorganism fungus kind is made through first order seed culture, secondary seed culture and liquid fermentation and culture
Zymocyte liquid；
A1, first order seed culture：Microorganism fungus kind inclined-plane is inoculated into first order seed culture in the ratio of ring/80-120mL
In base, fermentation obtains primary seed solution；
A2, secondary seed culture：By primary seed solution by weight 5-15:100 are inoculated into secondary seed medium, hair
Ferment obtains secondary seed solution；
A3, liquid fermentation and culture：By secondary seed solution by weight 5-15:100 are inoculated into liquid fermentation medium, hair
Zymocyte liquid is made in ferment；
B, inoculation fermentation：By fish meal by weight 100:Zymocyte liquid made from 30-50 inoculation steps A, after inoculation
Fish meal is fitted into packaging bag, and a pellosil that can adjust air pressure is added in packaging bag, storage fermentation at normal temperatures after sealing,
Until pH is down to 3.0-4.0, high digestibility fermentation fish meal is made.
The preparation method of the present invention is made high digestibility fermentation fish meal, contains by the way that fish meal is undergone microbial fermentation
A large amount of beneficial microbe can improve the digestibility of protein；And fermentation process generate metabolite and to ferment bottom
The degradation of object so that the ingredient of high digestibility fermentation fish meal is extremely complex, improves the quality of fish meal protein.
The high digestibility fermentation fish meal of tradition is all first to make product, then pack.Pellosil packaged type anaerobic solid-state fermentation
Technique is that packaging bag is designed to small-sized fermentation tank, using zymotechnique after first packing.Silica gel adapted film balances creatively solve
It has determined the technical barriers such as gas control and living contaminants in fermentation production process.During the fermentation, microbes generate dioxy
Change the gases such as carbon so that the air pressure in bag is more than extraneous normal pressure.When air pressure reaches a certain critical value in bag, gas passes through this
Pellosil is discharged to the external world.But extraneous gas has no chance to enter fermentation system always, so as to also just eliminate the miscellaneous of the external world
Bacterium interferes.
The present invention is not needed to by using respiratory membrane packaged type anaerobic solid-state fermentation technique, fermentation process into trip temperature tune
Section, it is also not influenced by ambient temperature.The fermenting-ripening required time changes with environment temperature and material composition, but the shelf-life
Not by time restriction, as long as packaging bag is intact, the shelf-life can reach 3 years.
The preparation method of the present invention is easy to operate, if with 30 tons of calculating of daily output, equipment investment is no more than 200,000 yuan, especially
It is suitble to middle-size and small-size use.
Preferably, in the step A1, microorganism fungus kind includes the raw material of following parts by weight：20-30 parts of S. cervisiae,
8-12 parts of 15-25 parts of Lactobacillus casei, 10-20 parts of bacillus subtilis, 5-15 parts of actinomyces and photosynthetic bacteria.
For the microorganism fungus kind of the present invention by using S. cervisiae, it can utilize carbon source in culture medium, photosynthetic bacteria
Amino acid, carbohydrate and the other organic substances of synthesis generate fermenting power, for promoting other effective microbe (such as cheese breast bars
Bacterium, actinomyces) the important subsistence support of required matrix (food) offer of proliferation；Saccharomycete exists for doing as aerobic bacteria
The growth and breeding of Lactobacillus paracasei creates anaerobic environment, in addition, the single cell protein that saccharomycete generates is that animal is indispensable
Nutrient.
By using Lactobacillus casei, it is generated the microorganism fungus kind of the present invention by intake photosynthetic bacteria, S. cervisiae
Carbohydrate form lactic acid, lactic acid has very strong sterilizing ability, can effectively inhibit the urgency of harmful microbe activity and organic matter
Acute corruption is decomposed.Lactobacillus casei amount reproduction generates lactic acid, reduces pH value, this allows for high digestibility fermentation fish meal finished product
With strong sour fragrance, so as to improve palatability, the feed intake of animal is provided.
The microorganism fungus kind of the present invention is by using bacillus subtilis, its available protein, a variety of sugar and starch,
Subtilin, polymyxins, nystatin, the gramicidins isoreactivity substance generated in growth course, these active materials pair
Pathogenic bacteria have apparent inhibiting effect；Bacillus can efficiently kill Escherichia coli, salmonella and staphylococcus aureus
Harmful microorganisms are waited, but the growth metabolism of Lactobacillus casei and S. cervisiae is not influenced；Bacillus subtilis conduct
The growth and breeding for existing for lactic acid bacteria of aerobic bacteria creates anaerobic environment.
For the microorganism fungus kind of the present invention by using actinomyces, it obtains the conducts such as amino acid, nitrogen from photosynthetic bacteria
Matrix produces various antibiotics, vitamin and enzyme, can directly inhibit pathogen.Actinomyces obtain harmful mould in advance
With the required matrix of bacterial multiplication, so as to inhibit their proliferation, and the existence ring of other beneficial microbe proliferation is createed
Border.The substance decomposed after actinomyces and photosynthetic bacteria mixing to hardly possible, such as lignin, cellulose, chitin have degradation,
And easily absorbed by animal, enhancing animal is to the resistance and immunity of various diseases.
The microorganism fungus kind of the present invention belongs to autotrophy microorganism, thalline contains in itself by using photosynthetic bacteria
More than 60% protein, and rich in multivitamin, also containing Co-Q10, antiviral substance and somatomedin；It is with light
It is the energy with heat, is matrix by organic matter, carbon dioxide, nitrogen etc., synthesizes carbohydrate, amino acids, vitamins, nitrogen chemical combination
Object, antiviral substance and physiological activator etc. are the main powers for promoting growth of animal.
The present invention microorganism fungus kind by using S. cervisiae, Lactobacillus casei, bacillus subtilis, actinomyces and
Photosynthetic bacteria compounds, and strictly controls its weight proportion, combines anaerobic bacteria and the advantages of aerobic bacteria ferments, raw material does not need to disappear
Poison can be directly used in inoculated and cultured；And each quasi-microorganism all respectively plays an important role, central role is photosynthetic bacteria
It is leading with Lactobacillus casei, synthesis capability supports the activity of other microorganisms, while is also generated using other microorganisms
Substance, form the relationship of coexistence and co-prosperity, ensure that the microorganism fungus kind of the present invention is in stable condition, it is multiple functional, given play to compounding
Optimum efficiency.
The microorganism fungus kind of the present invention can generate antioxidant, remove oxidation material, eliminate corruption, inhibit pathogen,
Form the good environment suitable for growth of animal, meanwhile, it also generates a large amount of benefit materials easily absorbed for animal, as amino acid,
Organic acid, Polysaccharides, various vitamins, various biochemical enzymes, somatomedin, antibiotic and antiviral substance etc. improve animal
Immune function, promote healthy growth.
The microorganism fungus kind of the present invention does not generate poisonous and harmful substance, and will not endanger the intrinsic ecology of environment and put down in itself
Weighing apparatus.The microorganism fungus kind of the present invention has fine growth metabolism vigor in itself, and can effectively degrade macromolecular and anti-nutritional factors,
Synthesize the small-molecule substances such as small peptide and organic acid；And can protect and strengthen animal body microflora balance, promote animal health.
Preferably, in the step A1, the preparation method of primary-seed medium includes the following steps：Take peptone 12-
16g is dissolved with 500mL hot water, is separately added into beef extract 11-15g, yeast extract 11-15g, glucose 6-8g, brewer's wort culture
Base 2-4g, sodium acetate 2-4g, ammonium citrate 0.5-1.5g, potassium dihydrogen phosphate 0.5-1.5g, magnesium sulfate 0.3-0.5g and tween
0.4-0.8g, benefit are filled with water to 1L, and pH value is adjusted to be dispensed after dissolving, per the bottled liquid 500- of 1000mL triangles to 6.0-7.0
700mL at a temperature of 110-130 DEG C, carries out high pressure sterilization 20-40min, is cooled to 20-30 DEG C, obtains under 0.1-0.2MPa pressure
To primary-seed medium.
Preferably, in the step A1, the condition of first order seed culture：Fermentation temperature is 28-32 DEG C, and shaking speed is
150-250r/min is sealed by fermentation 3-5d, measures pH value daily and discharges the gas of generation, when pH value is down to 3.0-4.0
Stop fermentation.
Preferably, in the step A2, the preparation method of secondary seed medium includes the following steps：Take whole milk powder
12-16g is separately added into beef extract 11-15g, yeast extract 11-15g, malt extract 4-8g, glucose with 500mL hot water dissolvings
6-8g, cane molasses 8-12g, sodium acetate 2-4g, ammonium citrate 0.5-1.5g, potassium dihydrogen phosphate 0.5-1.5g, magnesium sulfate 0.3-
0.5g and tween 0.4-0.8g, benefit are filled with water to 1L, and pH value is adjusted to be dispensed after dissolving, every 1000mL triangles are bottled to 6.0-7.0
Liquid 500-700mL at a temperature of 110-130 DEG C, carries out high pressure sterilization 20-40min, is cooled to 20- under 0.1-0.2MPa pressure
30 DEG C, obtain secondary seed medium.
Preferably, in the step A2, the condition of secondary seed culture：Fermentation temperature is 28-32 DEG C, and shaking speed is
150-250r/min is sealed by fermentation 5-7d, measures pH value daily and discharges the gas of generation, when pH value is down to 3.0-4.0
Stop fermentation.
Preferably, in the step A3, the preparation method of liquid fermentation medium includes the following steps：Take peptone 3-5g
With 500mL hot water dissolvings, it is separately added into glucose 10-20g, beef extract 3-5g, bean cake powder 3-5g, cornstarch 2-4g, phytic acid
Enzyme 1-3g, potassium dihydrogen phosphate 0.5-1.5g, magnesium phosphate 0.3-0.5g, sodium chloride 0.5-1.5g and tween 0.4-0.8g supplement water
To 1L, pH value is adjusted to be dispensed after dissolving, per the 1000mL bottled liquid 500-700mL of triangle, in 110-130 DEG C of temperature to 6.0-7.0
Under degree, high pressure sterilization 20-40min is carried out under 0.1-0.2MPa pressure, 20-30 DEG C is cooled to, obtains liquid fermentation medium.
Preferably, in the step A3, the condition of liquid fermentation and culture：Fermentation temperature is 28-32 DEG C, and shaking speed is
150-250r/min is sealed by fermentation 3-5d, measures pH value daily and discharges the gas of generation, when pH value is down to 3.0-4.0
Stop fermentation.
Preferably, in the step B, added with the fulvic acid for accounting for fish meal weight 1%-2% in fish meal.
The present invention controls the 1%-2% that its dosage is fish meal weight by using fulvic acid, has hemostasis, anti-inflammatory, receipts
It holds back, adsorb, antiallergy, promote secrete, remove necrosis and promote granulation, adjust gastrointestinal function, improving immunity of organisms and other effects.Fulvic acid is both battalion
Substance is supported, and is class growth hormone, to improving feed nutrition, promote growth of animal, increases the disease resistance of body and anti-oxidant
Power, treatment viral infectious aspect have very obvious action.
Another object of the present invention is achieved through the following technical solutions：A kind of high digestibility fermentation fish meal, the high digestion
Rate fermentation fish meal is made according to preparation method described above.
The high digestibility fermentation fish meal of the present invention greatly reduces abnormal fermentation of the feed in large intestine, so as to reducing or
Eliminate trophism diarrhea；Significantly improve the absorption rate of amino acid and mineral matter element；Significantly improve pancreas in animal blood
Island element is horizontal, promotes lymphocytosis, and it is horizontal to improve animal immune；It can inhibit the breeding of harmful bacteria in enteron aisle, keep enteron aisle
The micro-ecological environment of health improves animal small intestine function, even more so that high digestibility fermentation fish meal polypeptide portion substitute antibiotics
It is possibly realized；The digestibility of feed can be improved.
The beneficial effects of the present invention are：Height is made by the way that fish meal is undergone microbial fermentation in the preparation method of the present invention
Digestibility fermentation fish meal containing a large amount of beneficial microbe, can improve the digestibility of protein；And fermentation process generation
Metabolite and the degradation to fermentation substrate so that the ingredient of high digestibility fermentation fish meal is extremely complex, improves fish
The quality of amyloid proteins.
The high digestibility fermentation fish meal protein digestibility of the present invention is up to more than 98%, greatly reduces feed big
Abnormal fermentation in intestines, so as to reduce or eliminate trophism diarrhea；Polypeptide content is more than 70%, significantly improves amino acid
With the absorption rate of mineral matter element；Biologically active small peptide can significantly provide insulin level in animal blood,
Promote lymphocytosis, it is horizontal to improve animal immune；Probiotics and lactic acid can inhibit the breeding of harmful bacteria in enteron aisle, keep
The micro-ecological environment of intestinal health improves animal small intestine function, resists even more so that high digestibility fermentation fish meal polypeptide portion substitutes
Raw element is possibly realized；Microbial metabolic products such as digestive ferment can improve the digestibility of feed.Also have in microbe metabolite to dynamic
Unknown growth factor, vitamin of the direct trophism of object etc..On the other hand, the solubility of fish meal polypeptide products is high, viscosity is low,
The excellent substance characteristics such as anti-gel formative is good make as caused by stickiness component (such as the wheats such as wheat-middlings, wheat bran class) to resist in daily ration
Trophism disappears so that high digestibility fermentation fish meal polypeptide portion substitutes complex enzyme for feed, Tiny ecosystem is possibly realized.
The high digestibility fermentation fish meal of the present invention kills the harmful microorganisms such as bacterium, fungi, disappears by using zymotechnique
Except the characteristic odor of fish meal product, degradation fish meal protein is the products such as small molecular protein, small peptide.The high digestibility fermentation of the present invention
Fish meal shows preferable utility value, in animal and fowl fodder and aquatic feeds as a kind of good animal protein raw material
Additive amount can arrive 3%-10%, show better growth-promoting effect.
Specific embodiment
For the ease of the understanding of those skilled in the art, with reference to embodiment, the present invention is further illustrated, real
The content that the mode of applying refers to not is limitation of the invention.
Embodiment 1
A kind of preparation method of high digestibility fermentation fish meal, includes the following steps：
A, prepared by strain：Microorganism fungus kind is made through first order seed culture, secondary seed culture and liquid fermentation and culture
Zymocyte liquid；
A1, first order seed culture：Microorganism fungus kind inclined-plane is inoculated into primary-seed medium in the ratio of ring/80mL
In, fermentation obtains primary seed solution；
A2, secondary seed culture：By primary seed solution by weight 5:100 are inoculated into secondary seed medium, fermentation
Obtain secondary seed solution；
A3, liquid fermentation and culture：By secondary seed solution by weight 5:100 are inoculated into liquid fermentation medium, fermentation
Zymocyte liquid is made；
B, inoculation fermentation：By fish meal by weight 100:Zymocyte liquid made from 30 inoculation step A, by the fish meal after inoculation
It is fitted into packaging bag, a pellosil that can adjust air pressure is added in packaging bag, storage fermentation at normal temperatures after sealing, until
PH is down to 3.0, and high digestibility fermentation fish meal is made.
In the step A1, microorganism fungus kind includes the raw material of following parts by weight：20 parts of S. cervisiae, Lactobacillus casei
8 parts of 15 parts, 10 parts of bacillus subtilis, 5 parts of actinomyces and photosynthetic bacteria.
In the step A1, the preparation method of primary-seed medium includes the following steps：Take peptone 12g 500mL
Hot water dissolves, and is separately added into beef extract 11g, yeast extract 11g, glucose 6g, malt extract medium 2g, sodium acetate 2g, lemon
Sour ammonium 0.5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.3g and tween 0.4g, benefit are filled with water to 1L, and adjusting pH value, dissolving is laggard to 6.0
Row packing per 1000mL bottled liquid 500mL of triangle, carries out high pressure sterilization 20min at a temperature of 110 DEG C, under 0.1MPa pressure, cold
But to 20 DEG C, primary-seed medium is obtained.
In the step A1, the condition of first order seed culture：Fermentation temperature is 28 DEG C, shaking speed 150r/min, close
Seal ferment 3d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0.
In the step A2, the preparation method of secondary seed medium includes the following steps：Whole milk powder 12g is taken to use
500mL hot water dissolvings are separately added into beef extract 11g, yeast extract 11g, malt extract 4g, glucose 6g, cane molasses 8g, acetic acid
Sodium 2g, ammonium citrate 0.5g, potassium dihydrogen phosphate 0.5g, magnesium sulfate 0.3g and tween 0.4g are mended and are filled with water to 1L, tune pH value to 6.0,
It is dispensed after dissolving, per the 1000mL bottled liquid 500mL of triangle, high pressure sterilization is carried out at a temperature of 110 DEG C, under 0.1MPa pressure
20min is cooled to 20 DEG C, obtains secondary seed medium.
In the step A2, the condition of secondary seed culture：Fermentation temperature is 28 DEG C, shaking speed 150r/min, close
Seal ferment 5d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0.
In the step A3, the preparation method of liquid fermentation medium includes the following steps：Take peptone 3g 500mL heat
Water dissolution is separately added into glucose 10g, beef extract 3g, bean cake powder 3g, cornstarch 2g, phytase 1g, potassium dihydrogen phosphate
0.5g, magnesium phosphate 0.3g, sodium chloride 0.5g and tween 0.4g, benefit are filled with water to 1L, and pH value is adjusted to be dispensed after dissolving, often to 6.0
The bottled liquid 500mL of 1000mL triangles at a temperature of 110 DEG C, carries out high pressure sterilization 20min, is cooled to 20 DEG C under 0.1MPa pressure,
Obtain liquid fermentation medium.
In the step A3, the condition of liquid fermentation and culture：Fermentation temperature is 28 DEG C, shaking speed 150r/min, close
Seal ferment 3d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0.
In the step B, added with the fulvic acid for accounting for fish meal weight 1% in fish meal.
A kind of high digestibility fermentation fish meal, the high digestibility fermentation fish meal are made according to preparation method described above.
Embodiment 2
A kind of preparation method of high digestibility fermentation fish meal, includes the following steps：
A, prepared by strain：Microorganism fungus kind is made through first order seed culture, secondary seed culture and liquid fermentation and culture
Zymocyte liquid；
A1, first order seed culture：Microorganism fungus kind inclined-plane is inoculated into primary-seed medium in the ratio of ring/90mL
In, fermentation obtains primary seed solution；
A2, secondary seed culture：By primary seed solution by weight 7:100 are inoculated into secondary seed medium, fermentation
Obtain secondary seed solution；
A3, liquid fermentation and culture：By secondary seed solution by weight 7:100 are inoculated into liquid fermentation medium, fermentation
Zymocyte liquid is made；
B, inoculation fermentation：By fish meal by weight 100:Zymocyte liquid made from 35 inoculation step A, by the fish meal after inoculation
It is fitted into packaging bag, a pellosil that can adjust air pressure is added in packaging bag, storage fermentation at normal temperatures after sealing, until
PH is down to 3, and high digestibility fermentation fish meal is made.
In the step A1, microorganism fungus kind includes the raw material of following parts by weight：22 parts of S. cervisiae, Lactobacillus casei
9 parts of 17 parts, 12 parts of bacillus subtilis, 7 parts of actinomyces and photosynthetic bacteria.
In the step A1, the preparation method of primary-seed medium includes the following steps：Take peptone 13g 500mL
Hot water dissolves, and is separately added into beef extract 12g, yeast extract 12g, glucose 6g, malt extract medium 2g, sodium acetate 2g, lemon
Sour ammonium 0.7g, potassium dihydrogen phosphate 0.7g, magnesium sulfate 0.35g and tween 0.5g, benefit are filled with water to 1L, and adjusting pH value, dissolving is laggard to 6.0
Row packing per the 1000mL bottled liquid 550mL of triangle, at a temperature of 115 DEG C, carries out high pressure sterilization 25min under 0.12MPa pressure,
22 DEG C are cooled to, obtains primary-seed medium.
In the step A1, the condition of first order seed culture：Fermentation temperature is 29 DEG C, shaking speed 175r/min, close
Seal ferment 3.5d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0.
In the step A2, the preparation method of secondary seed medium includes the following steps：Whole milk powder 13g is taken to use
500mL hot water dissolvings are separately added into beef extract 12g, yeast extract 12g, malt extract 5g, glucose 6.5g, cane molasses 9g, second
Sour sodium 2.5g, ammonium citrate 0.7g, potassium dihydrogen phosphate 0.7g, magnesium sulfate 0.35g and tween 0.5g, benefit are filled with water to 1L, adjust pH value
To 6.0, dispensed after dissolving, per the 1000mL bottled liquid 550mL of triangle, carried out at a temperature of 115 DEG C, under 0.15MPa pressure
High pressure sterilization 25min is cooled to 22 DEG C, obtains secondary seed medium.
In the step A2, the condition of secondary seed culture：Fermentation temperature is 29 DEG C, shaking speed 175r/min, close
Seal ferment 5.5d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0.
In the step A3, the preparation method of liquid fermentation medium includes the following steps：Take peptone 3.5g 500mL
Hot water dissolving is separately added into glucose 12g, beef extract 3.5g, bean cake powder 3.5g, cornstarch 2.5g, phytase 1.5g, phosphoric acid
Potassium dihydrogen 0.7g, magnesium phosphate 0.35g, sodium chloride 0.7g and tween 0.5g, benefit are filled with water to 1L, and pH value is adjusted to be carried out after dissolving to 6.0
Packing per 1000mL bottled liquid 550mL of triangle, carries out high pressure sterilization 25min at a temperature of 115 DEG C, under 0.12MPa pressure, cold
But to 22 DEG C, liquid fermentation medium is obtained.
In the step A3, the condition of liquid fermentation and culture：Fermentation temperature is 29 DEG C, shaking speed 175r/min, close
Seal ferment 3.5d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0.
In the step B, added with the fulvic acid for accounting for fish meal weight 1.2% in fish meal.
A kind of high digestibility fermentation fish meal, the high digestibility fermentation fish meal are made according to preparation method described above.
Embodiment 3
A kind of preparation method of high digestibility fermentation fish meal, includes the following steps：
A, prepared by strain：Microorganism fungus kind is made through first order seed culture, secondary seed culture and liquid fermentation and culture
Zymocyte liquid；
A1, first order seed culture：Microorganism fungus kind inclined-plane is inoculated into primary-seed medium in the ratio of ring/100mL
In, fermentation obtains primary seed solution；
A2, secondary seed culture：By primary seed solution by weight 10:100 are inoculated into secondary seed medium, fermentation
Obtain secondary seed solution；
A3, liquid fermentation and culture：By secondary seed solution by weight 10:100 are inoculated into liquid fermentation medium, fermentation
Zymocyte liquid is made；
B, inoculation fermentation：By fish meal by weight 100:Zymocyte liquid made from 40 inoculation step A, by the fish meal after inoculation
It is fitted into packaging bag, a pellosil that can adjust air pressure is added in packaging bag, storage fermentation at normal temperatures after sealing, until
PH is down to 3.0, and high digestibility fermentation fish meal is made.
In the step A1, microorganism fungus kind includes the raw material of following parts by weight：25 parts of S. cervisiae, Lactobacillus casei
10 parts of 20 parts, 15 parts of bacillus subtilis, 10 parts of actinomyces and photosynthetic bacteria.
In the step A1, the preparation method of primary-seed medium includes the following steps：Take peptone 14g 500mL
Hot water dissolves, and is separately added into beef extract 13g, yeast extract 13g, glucose 7g, malt extract medium 3g, sodium acetate 3g, lemon
Sour ammonium 1g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.4g and tween 0.6g, benefit are filled with water to 1L, and pH value is adjusted to be divided after dissolving to 6.0
Dress per the 1000mL bottled liquid 600mL of triangle, at a temperature of 120 DEG C, carries out high pressure sterilization 30min, cooling under 0.15MPa pressure
To 25 DEG C, primary-seed medium is obtained.
In the step A1, the condition of first order seed culture：Fermentation temperature is 30 DEG C, shaking speed 200r/min, close
Seal ferment 4d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0.
In the step A2, the preparation method of secondary seed medium includes the following steps：Whole milk powder 14g is taken to use
500mL hot water dissolvings are separately added into beef extract 13g, yeast extract 13g, malt extract 6g, glucose 7g, cane molasses 10g, second
Sour sodium 3g, ammonium citrate 1g, potassium dihydrogen phosphate 1g, magnesium sulfate 0.4g and tween 0.6g, benefit are filled with water to 1L, adjust pH value to 6.0, molten
It is dispensed after solution, per the 1000mL bottled liquid 600mL of triangle, high pressure sterilization is carried out at a temperature of 120 DEG C, under 0.15MPa pressure
30min is cooled to 25 DEG C, obtains secondary seed medium.
In the step A2, the condition of secondary seed culture：Fermentation temperature is 30 DEG C, shaking speed 200r/min, close
Seal ferment 6d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0.
In the step A3, the preparation method of liquid fermentation medium includes the following steps：Take peptone 4g 500mL heat
Water dissolution, be separately added into glucose 15g, beef extract 4g, bean cake powder 4g, cornstarch 3g, phytase 2g, potassium dihydrogen phosphate 1g,
Magnesium phosphate 0.4g, sodium chloride 1g and tween 0.6g, benefit are filled with water to 1L, and pH value is adjusted to be dispensed after dissolving, per 1000mL to 6.0
The bottled liquid 600mL of triangle at a temperature of 120 DEG C, carries out high pressure sterilization 30min, is cooled to 25 DEG C, obtains under 0.15MPa pressure
Liquid fermentation medium.
In the step A3, the condition of liquid fermentation and culture：Fermentation temperature is 30 DEG C, shaking speed 200r/min, close
Seal ferment 4d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0.
In the step B, added with the fulvic acid for accounting for fish meal weight 1.5% in fish meal.
A kind of high digestibility fermentation fish meal, the high digestibility fermentation fish meal are made according to preparation method described above.
Embodiment 4
A kind of preparation method of high digestibility fermentation fish meal, includes the following steps：
A, prepared by strain：Microorganism fungus kind is made through first order seed culture, secondary seed culture and liquid fermentation and culture
Zymocyte liquid；
A1, first order seed culture：Microorganism fungus kind inclined-plane is inoculated into primary-seed medium in the ratio of ring/110mL
In, fermentation obtains primary seed solution；
A2, secondary seed culture：By primary seed solution by weight 12:100 are inoculated into secondary seed medium, fermentation
Obtain secondary seed solution；
A3, liquid fermentation and culture：By secondary seed solution by weight 12:100 are inoculated into liquid fermentation medium, fermentation
Zymocyte liquid is made；
B, inoculation fermentation：By fish meal by weight 100:Zymocyte liquid made from 45 inoculation step A, by the fish meal after inoculation
It is fitted into packaging bag, a pellosil that can adjust air pressure is added in packaging bag, storage fermentation at normal temperatures after sealing, until
PH is down to 4.0, and high digestibility fermentation fish meal is made.
In the step A1, microorganism fungus kind includes the raw material of following parts by weight：28 parts of S. cervisiae, Lactobacillus casei
11 parts of 22 parts, 18 parts of bacillus subtilis, 12 parts of actinomyces and photosynthetic bacteria.
In the step A1, the preparation method of primary-seed medium includes the following steps：Take peptone 15g 500mL
Hot water dissolves, be separately added into beef extract 14g, yeast extract 14g, glucose 7g, malt extract medium 3.5g, sodium acetate 3.5g,
Ammonium citrate 1.2g, potassium dihydrogen phosphate 1.2g, magnesium sulfate 0.45g and tween 0.7g, benefit are filled with water to 1L, and pH value is adjusted to be dissolved to 7.0
After dispensed, per 1000mL bottled liquid 650mL of triangle, high pressure sterilization is carried out at a temperature of 125 DEG C, under 0.18MPa pressure
35min is cooled to 28 DEG C, obtains primary-seed medium.
In the step A1, the condition of first order seed culture：Fermentation temperature is 31 DEG C, shaking speed 225r/min, close
Seal ferment 4.5d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 4.0.
In the step A2, the preparation method of secondary seed medium includes the following steps：Whole milk powder 15g is taken to use
500mL hot water dissolvings are separately added into beef extract 14g, yeast extract 14g, malt extract 7g, glucose 7g, cane molasses 11g, second
Sour sodium 3.5g, ammonium citrate 1.2g, potassium dihydrogen phosphate 1.2g, magnesium sulfate 0.45g and tween 0.7g, benefit are filled with water to 1L, adjust pH value
To 7.0, dispensed after dissolving, per the 1000mL bottled liquid 650mL of triangle, carried out at a temperature of 125 DEG C, under 0.18MPa pressure
High pressure sterilization 35min is cooled to 28 DEG C, obtains secondary seed medium.
In the step A2, the condition of secondary seed culture：Fermentation temperature is 31 DEG C, shaking speed 225r/min, close
Seal ferment 6.5d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 4.0.
In the step A3, the preparation method of liquid fermentation medium includes the following steps：Take peptone 4.5g 500mL
Hot water dissolving is separately added into glucose 18g, beef extract 4.5g, bean cake powder 4.5g, cornstarch 3.5g, phytase 2.5g, phosphoric acid
Potassium dihydrogen 1.2g, magnesium phosphate 0.45g, sodium chloride 1.2g and tween 0.7g, benefit are filled with water to 1L, and pH value is adjusted to be carried out after dissolving to 7.0
Packing per 1000mL bottled liquid 650mL of triangle, carries out high pressure sterilization 35min at a temperature of 125 DEG C, under 0.18MPa pressure, cold
But to 28 DEG C, liquid fermentation medium is obtained.
In the step A3, the condition of liquid fermentation and culture：Fermentation temperature is 31 DEG C, shaking speed 225r/min, close
Seal ferment 4.5d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 4.0.
In the step B, added with the fulvic acid for accounting for fish meal weight 1.8% in fish meal.
A kind of high digestibility fermentation fish meal, the high digestibility fermentation fish meal are made according to preparation method described above.
Embodiment 5
A kind of preparation method of high digestibility fermentation fish meal, includes the following steps：
A, prepared by strain：Microorganism fungus kind is made through first order seed culture, secondary seed culture and liquid fermentation and culture
Zymocyte liquid；
A1, first order seed culture：Microorganism fungus kind inclined-plane is inoculated into primary-seed medium in the ratio of ring/120mL
In, fermentation obtains primary seed solution；
A2, secondary seed culture：By primary seed solution by weight 15:100 are inoculated into secondary seed medium, fermentation
Obtain secondary seed solution；
A3, liquid fermentation and culture：By secondary seed solution by weight 15:100 are inoculated into liquid fermentation medium, fermentation
Zymocyte liquid is made；
B, inoculation fermentation：By fish meal by weight 100:Zymocyte liquid made from 50 inoculation step A, by the fish meal after inoculation
It is fitted into packaging bag, a pellosil that can adjust air pressure is added in packaging bag, storage fermentation at normal temperatures after sealing, until
PH is down to 4.0, and high digestibility fermentation fish meal is made.
In the step A1, microorganism fungus kind includes the raw material of following parts by weight：30 parts of S. cervisiae, Lactobacillus casei
12 parts of 25 parts, 20 parts of bacillus subtilis, 15 parts of actinomyces and photosynthetic bacteria.
In the step A1, the preparation method of primary-seed medium includes the following steps：Take peptone 16g 500mL
Hot water dissolves, and is separately added into beef extract 15g, yeast extract 15g, glucose 8g, malt extract medium 4g, sodium acetate 4g, lemon
Sour ammonium 1.5g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g and tween 0.8g, benefit are filled with water to 1L, and adjusting pH value, dissolving is laggard to 7.0
Row packing per 1000mL bottled liquid 700mL of triangle, carries out high pressure sterilization 40min at a temperature of 130 DEG C, under 0.2MPa pressure, cold
But to 30 DEG C, primary-seed medium is obtained.
In the step A1, the condition of first order seed culture：Fermentation temperature is 32 DEG C, shaking speed 250r/min, close
Seal ferment 5d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 4.0.
In the step A2, the preparation method of secondary seed medium includes the following steps：Whole milk powder 16g is taken to use
500mL hot water dissolvings are separately added into beef extract 15g, yeast extract 15g, malt extract 8g, glucose 8g, cane molasses 12g, second
Sour sodium 4g, ammonium citrate 1.5g, potassium dihydrogen phosphate 1.5g, magnesium sulfate 0.5g and tween 0.8g, benefit are filled with water to 1L, adjust pH value extremely
7.0, dispensed after dissolving, per 1000mL bottled liquid 700mL of triangle, at a temperature of 130 DEG C, under 0.2MPa pressure into horizontal high voltage
Sterilize 40min, is cooled to 30 DEG C, obtains secondary seed medium.
In the step A2, the condition of secondary seed culture：Fermentation temperature is 32 DEG C, shaking speed 250r/min, close
Seal ferment 7d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 4.0.
In the step A3, the preparation method of liquid fermentation medium includes the following steps：Take peptone 5g 500mL heat
Water dissolution is separately added into glucose 20g, beef extract 5g, bean cake powder 5g, cornstarch 4g, phytase 3g, potassium dihydrogen phosphate
1.5g, magnesium phosphate 0.5g, sodium chloride 1.5g and tween 0.8g, benefit are filled with water to 1L, and pH value is adjusted to be dispensed after dissolving, often to 7.0
The bottled liquid 700mL of 1000mL triangles at a temperature of 130 DEG C, carries out high pressure sterilization 40min, is cooled to 30 DEG C under 0.2MPa pressure,
Obtain liquid fermentation medium.
In the step A3, the condition of liquid fermentation and culture：Fermentation temperature is 32 DEG C, shaking speed 250r/min, close
Seal ferment 5d measures pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 4.0.
In the step B, added with the fulvic acid for accounting for fish meal weight 2% in fish meal.
A kind of high digestibility fermentation fish meal, the high digestibility fermentation fish meal are made according to preparation method described above.
The preparation method of the present invention is made high digestibility fermentation fish meal, contains by the way that fish meal is undergone microbial fermentation
A large amount of beneficial microbe can improve the digestibility of protein；And fermentation process generate metabolite and to ferment bottom
The degradation of object so that the ingredient of high digestibility fermentation fish meal is extremely complex, improves the quality of fish meal protein.
The high digestibility fermentation fish meal protein digestibility of the present invention is up to more than 98%, greatly reduces feed big
Abnormal fermentation in intestines, so as to reduce or eliminate trophism diarrhea；Polypeptide content is more than 70%, significantly improves amino acid
With the absorption rate of mineral matter element；Biologically active small peptide can significantly provide insulin level in animal blood,
Promote lymphocytosis, it is horizontal to improve animal immune；Probiotics and lactic acid can inhibit the breeding of harmful bacteria in enteron aisle, keep
The micro-ecological environment of intestinal health improves animal small intestine function, resists even more so that high digestibility fermentation fish meal polypeptide portion substitutes
Raw element is possibly realized；Microbial metabolic products such as digestive ferment can improve the digestibility of feed.Also have in microbe metabolite to dynamic
Unknown growth factor, vitamin of the direct trophism of object etc..On the other hand, the solubility of fish meal polypeptide products is high, viscosity is low,
The excellent substance characteristics such as anti-gel formative is good make as caused by stickiness component (such as the wheats such as wheat-middlings, wheat bran class) to resist in daily ration
Trophism disappears so that high digestibility fermentation fish meal polypeptide portion substitutes complex enzyme for feed, Tiny ecosystem is possibly realized.
The high digestibility fermentation fish meal of the present invention kills the harmful microorganisms such as bacterium, fungi, disappears by using zymotechnique
Except the characteristic odor of fish meal product, degradation fish meal protein is the products such as small molecular protein, small peptide.The high digestibility fermentation of the present invention
Fish meal shows preferable utility value, in animal and fowl fodder and aquatic feeds as a kind of good animal protein raw material
Additive amount can arrive 3-10%, show better growth-promoting effect.
Above-described embodiment is the preferable implementation of the present invention, and in addition to this, the present invention can be realized with other manner,
Any obvious replacement is not departed under the premise of present inventive concept within protection scope of the present invention.
Claims (7)
1. a kind of preparation method of high digestibility fermentation fish meal, it is characterised in that：Include the following steps：
A, prepared by strain：Fermentation is made through first order seed culture, secondary seed culture and liquid fermentation and culture in microorganism fungus kind
Bacterium solution；
A1, first order seed culture：Microorganism fungus kind inclined-plane is inoculated into primary-seed medium in the ratio of ring/80-120mL
In, fermentation obtains primary seed solution；
A2, secondary seed culture：By primary seed solution by weight 5-15:100 are inoculated into secondary seed medium, ferment
To secondary seed solution；
A3, liquid fermentation and culture：By secondary seed solution by weight 5-15:100 are inoculated into liquid fermentation medium, fermentation system
Obtain zymocyte liquid；
B, inoculation fermentation：By fish meal by weight 100:Zymocyte liquid made from 30-50 inoculation steps A, by the fish meal after inoculation
It is fitted into packaging bag, a pellosil that can adjust air pressure is added in packaging bag, storage fermentation at normal temperatures after sealing, until
PH is down to 3.0-4.0, and high digestibility fermentation fish meal is made；
In the step A1, microorganism fungus kind includes the raw material of following parts by weight：20-30 parts of S. cervisiae, Lactobacillus casei
8-12 parts of 15-25 parts, 10-20 parts of bacillus subtilis, 5-15 parts of actinomyces and photosynthetic bacteria；
In the step A3, the preparation method of liquid fermentation medium includes the following steps：Take peptone 3-5g 500mL hot water
Dissolving, is separately added into glucose 10-20g, beef extract 3-5g, bean cake powder 3-5g, cornstarch 2-4g, phytase 1-3g, phosphoric acid
Potassium dihydrogen 0.5-1.5g, magnesium phosphate 0.3-0.5g, sodium chloride 0.5-1.5g and tween 0.4-0.8g, benefit are filled with water to 1L, adjust pH value
It to 6.0-7.0, is dispensed after dissolving, per the 1000mL bottled liquid 500-700mL of triangle, at a temperature of 110-130 DEG C, 0.1-
High pressure sterilization 20-40min is carried out under 0.2MPa pressure, 20-30 DEG C is cooled to, obtains liquid fermentation medium；
In the step A3, the condition of liquid fermentation and culture：Fermentation temperature is 28-32 DEG C, shaking speed 150-250r/min,
3-5d is sealed by fermentation, measure pH value daily and discharges the gas of generation, stops fermentation when pH value is down to 3.0-4.0.
2. a kind of preparation method of high digestibility fermentation fish meal according to claim 1, it is characterised in that：The step A1
In, the preparation method of primary-seed medium includes the following steps：Peptone 12-16g is taken to be dissolved with 500mL hot water, is added respectively
Enter beef extract 11-15g, yeast extract 11-15g, glucose 6-8g, malt extract medium 2-4g, sodium acetate 2-4g, ammonium citrate
0.5-1.5g, potassium dihydrogen phosphate 0.5-1.5g, magnesium sulfate 0.3-0.5g and tween 0.4-0.8g, benefit are filled with water to 1L, adjust pH value extremely
6.0-7.0 is dispensed after dissolving, per the 1000mL bottled liquid 500-700mL of triangle, at a temperature of 110-130 DEG C, 0.1-
High pressure sterilization 20-40min is carried out under 0.2MPa pressure, 20-30 DEG C is cooled to, obtains primary-seed medium.
3. a kind of preparation method of high digestibility fermentation fish meal according to claim 1, it is characterised in that：The step A1
In, the condition of first order seed culture：Fermentation temperature is 28-32 DEG C, shaking speed 150-250r/min, is sealed by fermentation 3-5d,
It measures pH value daily and discharges the gas of generation, stop fermentation when pH value is down to 3.0-4.0.
4. a kind of preparation method of high digestibility fermentation fish meal according to claim 1, it is characterised in that：The step A2
In, the preparation method of secondary seed medium includes the following steps：Take whole milk powder 12-16g 500mL hot water dissolvings, difference
Add in beef extract 11-15g, yeast extract 11-15g, malt extract 4-8g, glucose 6-8g, cane molasses 8-12g, sodium acetate 2-
4g, ammonium citrate 0.5-1.5g, potassium dihydrogen phosphate 0.5-1.5g, magnesium sulfate 0.3-0.5g and tween 0.4-0.8g, benefit are filled with water to
1L adjusts pH value to be dispensed after dissolving, per the 1000mL bottled liquid 500-700mL of triangle, in 110-130 DEG C of temperature to 6.0-7.0
Under, under 0.1-0.2MPa pressure carry out high pressure sterilization 20-40min, be cooled to 20-30 DEG C, obtain secondary seed medium.
5. a kind of preparation method of high digestibility fermentation fish meal according to claim 1, it is characterised in that：The step A2
In, the condition of secondary seed culture：Fermentation temperature is 28-32 DEG C, shaking speed 150-250r/min, is sealed by fermentation 5-7d,
It measures pH value daily and discharges the gas of generation, stop fermentation when pH value is down to 3.0-4.0.
6. a kind of preparation method of high digestibility fermentation fish meal according to claim 1, it is characterised in that：The step B
In, added with the fulvic acid for accounting for fish meal weight 1%-2% in fish meal.
7. a kind of high digestibility fermentation fish meal, it is characterised in that：The high digestibility fermentation fish meal is any according to claim 1-6
Preparation method described in is made.
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| CN106173213A (en) * | 2016-07-06 | 2016-12-07 | 中国科学院合肥物质科学研究院 | A kind of manufacture method of fish flour |
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| CN102960541A (en) * | 2012-11-30 | 2013-03-13 | 张有聪 | Method for producing environment-friendly biological feed employing distiller grains and glycoprotein |
| CN102972635A (en) * | 2012-11-28 | 2013-03-20 | 张有聪 | Method for producing microorganism feed additive by utilizing probiotics and mixed meal |
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| CN102987057A (en) * | 2012-11-28 | 2013-03-27 | 张有聪 | Production method of biological fish meal through microbial fermentation |
| CN103598404A (en) * | 2013-11-01 | 2014-02-26 | 林豪杰 | Fish meal preparation method using EM bacteria, acid protease fermentation and enzymolysis |
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| CN102972635A (en) * | 2012-11-28 | 2013-03-20 | 张有聪 | Method for producing microorganism feed additive by utilizing probiotics and mixed meal |
| CN102987058A (en) * | 2012-11-28 | 2013-03-27 | 张有聪 | Method for producing efficient biological feed by using movable type fermentation and high-energy raw materials |
| CN102987057A (en) * | 2012-11-28 | 2013-03-27 | 张有聪 | Production method of biological fish meal through microbial fermentation |
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Address after: Room 1103, No. 4, Tutang Park Road, Changping Town, Dongguan City, Guangdong Province, 523000 Patentee after: DONGGUAN LINSHI BIOTECHNOLOGY CO.,LTD. Address before: Lin's Building, No. 2, Dajingjiu Agricultural and Sideline Products Wholesale Market, Xiakeng Village, Changping Town, Dongguan City, Guangdong Province, 523570 Patentee before: DONGGUAN LINSHI BIOTECHNOLOGY CO.,LTD. |