A kind of camellia radicle cottage method
[technical field]
The present invention relates to a kind of camellia radicle cottage method, belong to plant cultivation method technical field.
[background technology]
China is the distribution center of camellia, has abundant camellia germ plasm resource.Make a general survey of the development and utilization situation of camellia, only there is minority ornamental value high and adaptable camellia kind at present, as bright red in hexagonal, strong perfume (or spice) etc. is applied in family potted plant, garden and afforestation, the stage that most camellia kind is still in " support boudoir nobody knows ".Trace it to its cause, mainly the root system of most of camellia kind is undeveloped, high to growing environment requirement, and maintenance management level error is as easy as rolling off a log causes plant degeneration even dead.Utilize propagation method, improve its root system and improve its adaptive capacity to growing environment, greatly can promote the exploitation of camellia germ plasm resource.
[summary of the invention]
The present invention seeks to, in order to overcome the defect that existing camellia Technique on Cuttage Propagation survival rate is low, root of hair is slow, to provide a kind of survival rate high, the camellia radicle cottage method that root of hair is fast.
The present invention for achieving the above object, by the following technical solutions:
A kind of camellia radicle cottage method, is characterized in that comprising the following steps:
A, choose ripe Property of Tea oil Tree Fruit, artificial decortication, seed soaking, that vernalization to radicle stretches out at least 5cm is long, and epicotyl and plumule stretch out at least 3cm length altogether;
B, loose ventilative and the culture medium that water binding capacity is good evenly sprays the carbendazim of dilution 800 times, then use plastic film covering one week for subsequent use afterwards;
C, the Seed of Camellia oleifera of germination is cleaned up after, cut off epicotyl at nearly radicle place, cotyledon retains complete, and radicle retains 3 ~ 4cm, rives along radicle vertical line direction from epicotyl middle, and continues down to rive radicle;
D, scion one end is whittled into the wedge shape scion of two-sided smooth symmetry, each scion retains top 2 leaf, then scion is inserted radicle along plumule otch;
E, culture medium is watered permeable after, inserted in culture medium by the radicle of grafting, and airtight cultivation 25 ~ 30 days, to the Callus formation between scion and otch, the side root on radicle is formed, and can go up bag and cultivate.
The cuttage seeding root system adopting the radicle cuttage of Seed of Camellia oleifera to be formed in the present invention is powerful, preferably adopts Gaozhou county Seed of Camellia oleifera.
Culture medium in the present invention is by import peat: perlite by volume 3 ~ 4:1 forms, and this culture medium loosens ventilative and water binding capacity is good.
When processing chitting piece in the present invention, preferably cutting off epicotyl apart from radicle 0.4 ~ 0.6cm place, rive along radicle vertical line direction from epicotyl middle, and continue down to rive radicle, radicle incision length is 0.5 ~ 1cm, and when cutting off epicotyl, plumule excises.
The scion that the present invention chooses be camellia healthy and strong, without damage by disease and insect and the full branch of bud.
In the present invention, when scion is inserted along epicotyl otch, deeply to reach radicle and utilize radicle notch clip immediately fringe, fixing scion without the need to other materials.
In steps d of the present invention, 1/2 ~ 1/3 of its original area is trimmed to further to 2 pages, the top that scion retains, thus reduces scattering and disappearing of scion moisture; During grafting, scion is at least alignd with radicle, and the formation layer composition surface of the two is larger, is more beneficial to healing and survives.
The degree of depth that radicle after grafting inserts in culture medium is by the present invention: only stay the blade of scion and bud to be exposed on culture medium surface, other are all imbedded in culture medium.
The present invention compared with the prior art, has following advantage:
The cuttage seeding survival rate that the present invention is formed is high, root of hair is fast, utilizes the radicle of oil tea to carry out cuttage after grafting, can overcome the deficiency of traditional cottage method, make cuttage seeding well developed root system, the adaptive capacity enhancing etc. of growth rapidly, to growing environment.
The present invention is simple to operate, subtracts training cost, can continuous productive process.
[accompanying drawing explanation]
The schematic diagram that Fig. 1 is epicotyl of the present invention, radicle offers otch;
Fig. 2 is the schematic diagram that camellia scion access epicotyl and radicle form graft;
Fig. 3 is the schematic diagram that on radicle, new root is formed;
Position in diagram representated by label is as follows:
1-epicotyl, 2-radicle, 3-scion, 4-otch, 5-medium.
[embodiment]
Below in conjunction with specific embodiment, the invention will be further described:
Embodiment 1:
A, choose ripe Gaozhou county Property of Tea oil Tree Fruit, after artificial decortication, seed being placed in clean water soaks after 3 days, be embedded in river sand, sowing depth is 3 ~ 4cm, water permeable after, water weekly 1 ~ 2 time, to ensure that river sand has sufficient moisture to supply needed for seed sprouting, within about 30 ~ 40 days, germinate to Gaozhou county Seed of Camellia oleifera, it is long that radicle 2 stretches out 5cm, and it is long that epicotyl 1 and plumule stretch out 3cm;
B, by import peat and perlite by volume 4:1 be mixed into culture medium, culture medium evenly sprays the carbendazim of dilution 800 times, then uses plastic film covering for subsequent use after one week;
C, Gaozhou county Seed of Camellia oleifera of germination is cleaned up after, epicotyl 1 is cut off at nearly radicle 2 about 0.4 ~ 0.6cm place, cotyledon need retain complete, radicle 2 retains 3 ~ 4cm, rive along radicle 2 vertical line direction from epicotyl 1 middle, and continuing down to rive radicle 2, otch 4 length is about 0.5 ~ 1cm;
D, choose camellia kind " summer dream can be beautiful " healthy and strong, without the full branch of damage by disease and insect, bud as scion 3, scion 3 one end is whittled into the wedge shape scion of two-sided smooth symmetry, each scion 3 retains top 2 leaf, every leaf is all trimmed to 1/2 ~ 1/3 of its original area, then scion 3 is inserted radicle 2 along epicotyl 1 otch 4, and deeply reaching the notch clip immediately fringe 3 that radicle 2 utilizes radicle 2, scion 3 is at least alignd with radicle 2;
E, culture medium 5 is watered permeable after, to be inserted in culture medium 5 by the radicle 2 after grafting, then after watering a normal root water, airtight cultivation 25-30 days, the Callus formation between scion 3 and otch 4, the side root on radicle 2 is formed, and can go up bag and cultivate.
Graft survival rate and upper Pengcheng's motility rate of the camellia kind " summer dream can be beautiful " of embodiment 1 are as shown in table 1:
Table 1:
Embodiment 2:
A, choose ripe Property of Tea oil Tree Fruit, after artificial decortication, seed being placed in clean water soaks after 3 days, be embedded in river sand, sowing depth is 3 ~ 4cm, water permeable after, water weekly 1 ~ 2 time, to ensure that river sand has sufficient moisture to supply needed for seed sprouting, within about 30 ~ 40 days, germinate to Gaozhou county Seed of Camellia oleifera, it is long that radicle 2 stretches out 7cm, and it is long that epicotyl 1 and plumule stretch out 5cm;
B, by import peat and perlite by volume 3:1 be mixed into culture medium, culture medium evenly sprays the carbendazim of dilution 800 times, then uses plastic film covering for subsequent use after one week;
C, the Seed of Camellia oleifera of germination is cleaned up after, 0.4 ~ 0.6cm place of nearly radicle 2 cut off epicotyl 1, cotyledon need retain complete, radicle 2 retains 3 ~ 4cm, rive from epicotyl 1 middle along radicle 2 vertical line direction, and continue down to rive radicle 2, be about 0.5 ~ 1cm to otch 4 length;
D, choose camellia kind " summer Menghua woods " stalwartness, without the full branch of damage by disease and insect, bud as scion 3, scion 3 one end is whittled into the wedge shape scion of two-sided smooth symmetry, each scion 3 retains top 2 leaf, every leaf is all trimmed to 1/2 ~ 1/3 of its original area, then scion 3 is inserted radicle 2 along epicotyl 1 otch 4, and utilizing the notch clip immediately fringe 3 of radicle 2, scion 3 is at least alignd with radicle 2;
E, culture medium 5 is watered permeable after, to be inserted in culture medium 5 by the radicle 2 after grafting, then after watering a normal root water, airtight cultivation 25-30 days, the Callus formation between scion 3 and otch 4, the side root on radicle 2 is formed, and can go up bag and cultivate.
Graft survival rate and upper Pengcheng's motility rate of the camellia kind " summer Menghua woods " of embodiment 2 are as follows:
From embodiment 1 or 2, the cuttage seeding survival rate that the present invention is formed is high, is worthy to be popularized.