CN104904448A - Rapid breeding method for huperziaserrata seedlings - Google Patents
Rapid breeding method for huperziaserrata seedlings Download PDFInfo
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- CN104904448A CN104904448A CN201410452545.6A CN201410452545A CN104904448A CN 104904448 A CN104904448 A CN 104904448A CN 201410452545 A CN201410452545 A CN 201410452545A CN 104904448 A CN104904448 A CN 104904448A
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Abstract
The invention discloses a rapid breeding method for huperziaserrata seedlings. The method adopts spores as breeding materials for seedling culture. According to the method, huperziaserrata spore generation and spore seedling culture technology are mainly included. The method mainly comprises the steps of selecting and transplanting huperziaserrata adult plants, building a planting garden and a nursery garden, judging the spore maturity, cultivating and transplanting spore seedlings and the like. The artificial propagation method is utilized for enabling huperziaserrata adult plants to generate a large number of spores, the number of spores is much larger than the number of spores generated by plants in the wild environment naturally, spores are utilized for breeding, and a large number of huperziaserrata seedlings can be cultivated within the short time (two months). The spore breeding method is the unique and feasible method for cultivating huperziaserrata seedlings on a large scale at present.
Description
Technical field
The present invention relates to seedling breeding technical field, be specially Huperzia serrata brood cell technology and brood cell's seedling culture technology.
Background technology
Huperzia serrata
huperzia serrata(Thunb.ex Murray) Trevs, having another name called serrate clubmoss herb, be subordinate to Huperziaceae (Huperiaceae) stone araucaria, is a kind of short and small perennial plan pteridophyte, many growths covering, in moist, organic abundant environment, for China commonly uses Chinese traditional herbs.Huperzine contained by this plant has good result for the treatment of to alzheimer's disease and toxic and side effect is little, but is only 0.05% due to huperzine content in plant, and current raw material can not satisfy the demands far away.
Huperzia serrata growth is comparatively slow, average year growing height 2-3cm.Shortage of raw materials becomes the bottleneck that limit drug is produced.And the main cause of shortage of raw materials is that plant strain growth is slow, seedling breeding is difficult.Research finds that the main modes of reproduction of Huperzia serrata is sporogenesis and brood cell's breeding in wild environment, and these two kinds of modes all have the potentiality of seedling large-scale production, and people not yet grasp so far with spore is up to now the seedling breeding technology of material.And brood cell is as nourishing and generating material, its seedling has that sprout time is short, survival rate is high, and the fast feature of growth of seedling speed, can become the preferred manner of current scale seedling breeding.
Summary of the invention
The present invention adopts artificial cultivation strain, produces a large amount of brood cell, and carries out seedling fostering to brood cell.The invention solves the technical barrier of Huperzia serrata seedling breeding.
Huperzia serrata plant produces brood cell's technology and brood cell seedling culture technology, realizes by following steps.
Plant gathers: annual 4-5 month gathers in the wild and produces spore, well-grown plant, apart from plant 10cm place, deep-cuts 15cm, is with soil to dig out plant, do not destroy root system as far as possible, be loaded in plastic crate and be transported to plantation.
Plantation is built: with top layer, Huperzia serrata primary border 15cm soil for matrix, or with organic matter, gravel mixture (pH value 6.0) for matrix, after carrying out sterilization processing with carbendazim, matrix is laid on excessively drained field or on seedbed, stromal thickness 15cm.Above seedbed and surrounding set up the cool canopy of obscurity 75%, in canopy, ventilation equipment and spraying facility are installed.
Plantation: be planted in plantation by the plant of collection, dig the cave that 15cm is dark during plantation, plant and the subsidiary soil of root system are together put in cave, compacting gently, waters permeable after plantation, plantation specification 5cm × 5cm.
Plantation manages: after plantation, and keep plantation obscurity 75%, humidity more than 70%, and is ventilated 2 times every day, each 2 h.The plant planting after 1 year more than 90% produces brood cell, annual generation later 1 time.
Brood cell gathers: in annual 9-10 month, touch brood cell gently with round end tweezers, if brood cell easily comes off from plant, then show that brood cell is ripe, can gather.Do not allow caducous brood cell not yet ripe, be not suitable for gathering.The brood cell of collection is positioned in wide-mouth reagent bottle, transports nursery to.
Seedling bed is built: seedling bed matrix and environmental condition identical with plantation, seedbed nursery or seedling culture hole plate nursery, conveniently transplant and transport, generally employing seedling culture hole plate nursery in productions.Cave dish more than degree of depth 5cm, if lay seedbed, stromal thickness should at more than 5cm.
Sowing: the brood cell of collection answers sowing in time, the brood cell of collection is clamped brood cell gently with tweezers and is inserted in matrix gently downwards by brood cell's base portion, the degree of depth is 2/3 of brood cell's length, every cave kind 1 brood cell.If seedbed nursery, then planting specification is seeding row spacing 5cm × 5cm.
Seedling management: brood cell starts to take root after sowing 7d, sprouts.This stage all keeps seedbed humidity more than 70%, obscurity 75% before transplanting, and ventilates every day 2 times, each 2 h.
Transplant: select Proterozoic plantation brood cell seedling to be advisable, Proterozoic environmental condition is good, and growth of seedling is fast.After growth of seedling 60 d, can transplant during seedling height 3 about cm.If seedbed nursery, apart from seedling 2 cm during transplanting, deep-cut 3 cm, seedling is taken out, is put in the vinyl disc of the degree of depth 10 cm, be transported to and transplant ground, seedling leaves in transportation, should be kept moistening.If seedling culture hole plate nursery, then direct being transported to by seedling-cultivating tray is transplanted by matrix in cave and the complete taking-up of seedling behind ground, and in Proterozoic plantation, plantation specification 5cm × 5cm, waters permeable after plantation.
Further, described in step 6, gather the plant of the spore produced, only have strain to produce brood cell.
Described in step 10, touch brood cell gently with round end tweezers, brood cell easily comes off, then show ripe, otherwise immature.
Clamp brood cell's base portion with tweezers described in step 12, base portion inserts downwards in matrix, and brood cell's base portion is the position that brood cell is connected with plant.
Embodiment
Below in conjunction with example, the invention will be further described, but limited the present invention never in any form, and any conversion done based on the present invention or replacement, all belong to protection scope of the present invention.
The present invention specifically implements place: Xiushan Red Star traditional Chinese medicine Co., Ltd.
Realize especially by following technical scheme:
A. in May, 2010, each cities and counties and surrounding area gathered the Huperzia serrata strain having produced brood cell from Chongqing, deep-cut 15cm, taken out by plant together with soil during collection around plant;
B. seedbed is set up: the seedling growth greenhouse gross area 5000 ㎡, and obscurity 70%, humidity maintains 75-100%, has ventilation equipment in canopy, is provided with movable seedbed in canopy, and seedling culture hole plate is placed in seedbed.The organic matter of laying depth 20cm and gravel mixture on movable seedbed, and by carbendazim process matrix;
C. plant and plant: the strain of collection is directly planted on seedbed together with subsidiary soil, plantation specification seeding row spacing 5cm × 5cm, and water permeable;
D. seedbed management: keep obscurity 70%, humidity more than 75%, ventilate every day 4h, plants sum more than 90% plant after 1 year and all produce brood cell, annual generation later 1 time, and the plant quantity producing brood cell constantly increases;
E. brood cell gathers: the last ten-days period in September, selects the brood cell that born of the same parents' sheet is full, stirs brood cell gently with tweezers, collect the brood cell easily dropped, be loaded in wide-mouth bottle;
F. brood cell cultivates: be contained in degree of depth 5cm seedling culture hole plate by matrix that is organic and gravel mixing, seedling-cultivating tray is positioned on moveable seedbed, clamps the large born of the same parents' sheet of brood cell gently with tweezers, brood cell is inserted in matrix, the degree of depth is brood cell's 2/3, waters permeable with sprayer unit;
G. seedling management: keep obscurity 70%, humidity 75-100% in canopy, and 4 h that ventilate every day, the morning, afternoon each 2 h, after 7 d, brood cell starts to sprout young shoot, seedling height 3.5 cm after 60 d;
H seedling replanting: the cryptomeria underwood planting having wild Huperzia serrata to distribute in yellow water town, strata in Shizhu, Chongqing county the brood cell seedling of cultivation, plantation specification 5cm × 5cm.
In this example, seedling bed is in seedling raising greenhouse, and shade rate is fixed, and humidity is adjustable, the strain more than 200 of plantation Huperzia serrata plant, and strain all produces brood cell, and average every strain produces brood cell about 15, cultivates the strain more than 3000 of brood cell seedling altogether, seedling percent more than 95%.Brood cell's reproduction coefficient, far above cottage propagation, is the feasible method that uniquely can be used for the large-scale production of Huperzia serrata seedling at present.
Claims (13)
1. select spore-bearing Huperzia serrata plant in wild environment kind, with scuppit, plant root band soil is dug out together, excavate about degree of depth 15cm, keep root system complete as far as possible.
2. by the plantlet of transplant of collection in the plantation laid, plantation matrix is Huperzia serrata primary border 10 cm topsoil or organic matter, gravel mixture, pH value about 6.0, and uses carbendazim sterilizing; Above seedbed and surrounding set up cool canopy, shade rate 75%, have spraying facility in canopy to keep humidity more than 70%, have ventilation equipment.
3. become clump to plant every for the plant of collection 4 strain one clump of spacing in the rows 2cm, every clump of distance 15cm; Also can spacing in the rows, line-spacing 5cm evenly plant on seedbed; Keep environment light transmittance constant, humidity, more than 70%, is ventilated 2 times every day, each 2h, and plant strain growth started to generate brood cell after 1 year, often can produce brood cell every year later.
4. brood cell is general ripe in the fall, and maturation time is because of variant from different places; Touch the brood cell that plant produces gently with round end tweezers, if brood cell easily comes off from plant, brood cell is ripe, otherwise not yet ripe, and after immature brood cell's nursery, germination rate is low, and seedling growth is poor, easily dead; The brood cell of collection is put in wide-mouth bottle.
5. by gather brood cell's sowing in time on seedling-cultivating tray or seedbed, medium of seedling bed, environment are identical with plantation, but prepare the sterilizing of matrix application carbendazim; Can matrix be laid on seedbed, more than thickness 5cm, also can load seedling culture hole plate, dark about the 5cm in cave; Brood cell inserted gently in matrix, the degree of depth is 2/3 of brood cell's length, waters permeable with sprayer unit; If seedbed, plantation specification is 5cm × 5cm, if Pan Zemei cave, cave kind 1 brood cell.
6. in seedling raising process, seedbed shade rate is constant, and humid control, at 75-100%, ventilates 2 times every day, each 2 h.
7. after kind planting 7d, brood cell starts to take root, and grows blade, seedling height about 3cm after about 60 d, now can by seedling replanting to primary border, to promote growth of seedling; By long 2cm, wide 2cm, dark 3cm specification, seedling is dug out around seedling when getting seedling, keep root system complete, plantation specification 5cm × 5cm as far as possible; Selection has Huperzia serrata to distribute, and the primary condition block plantation of growing fine, water permeable after plantation.
8. according to claims 1, it is characterized in that: be captured in field and select spore-bearing plant, do not have spore-bearing plant to be shoot, after plantation for many years, just produce brood cell.
9. according to claims 2, it is characterized in that: matrix is primary border soil or organic, gravel mixture, and pH value is 6.0.
10. according to claims 3, it is characterized in that: keep environment light transmittance 75%, humidity, more than 70%, is ventilated 2 times every day, each 2h, and plant strain growth started to generate brood cell after 1 year, often can produce brood cell every year later; Environmental condition produces brood cell to plant and plays a decisive role.
11., according to claims 4, is characterized in that: touch brood cell gently with round end tweezers, if easily come off, brood cell is ripe, otherwise brood cell is immature, should not gather.
12., according to claims 5, is characterized in that: culture matrix is identical with described in claims 2, and brood cell's planting depth is 2/3 of the length of brood cell, is excessively deeply unfavorable for germination.
13., according to claims 7, is characterized in that: after seedling culture 60 d, under manual control condition, seedling can grow, but growth is comparatively slow, transplant and promote growth of seedling, primary border growth of seedling preferably to primary border.
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| CN201410452545.6A CN104904448A (en) | 2014-09-09 | 2014-09-09 | Rapid breeding method for huperziaserrata seedlings |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107548755A (en) * | 2017-10-09 | 2018-01-09 | 吉首大学 | A kind of primary border cuttage breeding method of Huperzia serrata |
| CN107616009A (en) * | 2017-10-20 | 2018-01-23 | 吉首大学 | A kind of primary border brood cell artificial fecundation method of Huperzia serrata |
| CN109526710A (en) * | 2018-07-26 | 2019-03-29 | 长沙慧日生物技术有限公司 | Serrate clubmoss herb and its method of soilless cultivation |
| CN110506517A (en) * | 2019-07-10 | 2019-11-29 | 广西壮族自治区林业科学研究院 | The method of Southern Pine cuttage seeding hayashishita scale breeding |
| CN114027041A (en) * | 2021-11-26 | 2022-02-11 | 浙江理工大学 | Efficient cuttage breeding method for huperzia serrata under natural condition |
-
2014
- 2014-09-09 CN CN201410452545.6A patent/CN104904448A/en active Pending
Non-Patent Citations (1)
| Title |
|---|
| 王德立等: "蛇足石杉的芽孢特性和芽孢繁育技术", 《安徽农业科学》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107548755A (en) * | 2017-10-09 | 2018-01-09 | 吉首大学 | A kind of primary border cuttage breeding method of Huperzia serrata |
| CN107616009A (en) * | 2017-10-20 | 2018-01-23 | 吉首大学 | A kind of primary border brood cell artificial fecundation method of Huperzia serrata |
| CN109526710A (en) * | 2018-07-26 | 2019-03-29 | 长沙慧日生物技术有限公司 | Serrate clubmoss herb and its method of soilless cultivation |
| CN109526710B (en) * | 2018-07-26 | 2021-04-30 | 皮剑波 | Huperzia serrata and soilless culture method thereof |
| CN110506517A (en) * | 2019-07-10 | 2019-11-29 | 广西壮族自治区林业科学研究院 | The method of Southern Pine cuttage seeding hayashishita scale breeding |
| CN110506517B (en) * | 2019-07-10 | 2021-08-13 | 广西壮族自治区林业科学研究院 | Method for understory scale breeding of southern pine cutting seedlings |
| CN114027041A (en) * | 2021-11-26 | 2022-02-11 | 浙江理工大学 | Efficient cuttage breeding method for huperzia serrata under natural condition |
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Application publication date: 20150916 |