CN104903464B - 用于从混合物富集突变核酸的化合物和方法 - Google Patents

用于从混合物富集突变核酸的化合物和方法 Download PDF

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CN104903464B
CN104903464B CN201380066924.5A CN201380066924A CN104903464B CN 104903464 B CN104903464 B CN 104903464B CN 201380066924 A CN201380066924 A CN 201380066924A CN 104903464 B CN104903464 B CN 104903464B
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allele
nucleic acid
mispairing
mutant
wild
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CN104903464A (zh
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A.古普塔
N.舍恩布伦纳
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F Hoffmann La Roche AG
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
    • C07F15/0006—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
    • C07F15/0073—Rhodium compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pyridine Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
CN201380066924.5A 2012-12-21 2013-12-19 用于从混合物富集突变核酸的化合物和方法 Active CN104903464B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261740895P 2012-12-21 2012-12-21
US61/740895 2012-12-21
PCT/EP2013/077282 WO2014096126A1 (en) 2012-12-21 2013-12-19 Compounds and methods for the enrichment of mutated nucleic acid from a mixture

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CN104903464A CN104903464A (zh) 2015-09-09
CN104903464B true CN104903464B (zh) 2018-04-24

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US (1) US9279146B2 (enExample)
EP (1) EP2935619B1 (enExample)
JP (1) JP2016508713A (enExample)
CN (1) CN104903464B (enExample)
CA (1) CA2894212C (enExample)
WO (1) WO2014096126A1 (enExample)

Families Citing this family (8)

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Publication number Priority date Publication date Assignee Title
EP3907297A1 (en) 2011-04-15 2021-11-10 The Johns Hopkins University Safe sequencing system
US11525163B2 (en) 2012-10-29 2022-12-13 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
SG11202001010UA (en) 2017-08-07 2020-03-30 Univ Johns Hopkins Methods and materials for assessing and treating cancer
EP3505643A1 (en) 2017-12-28 2019-07-03 Vito NV Methods for detecting nucleotide variants
CN108998504A (zh) * 2018-08-08 2018-12-14 鲍伟胜 一种提高基因突变检测灵敏度的方法
CA3170345A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids
GB2603203A (en) * 2021-02-02 2022-08-03 Univ Heriot Watt Method for enriching nucelic acids

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
US6120992A (en) * 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
WO2001005800A2 (en) * 1999-07-18 2001-01-25 Borries Kemper A method for the enrichment of heteroduplexes and its use in mutation detection
WO2007084380A2 (en) * 2006-01-13 2007-07-26 California Institute Of Technology Compounds and methods for nucleic acid mismatch detection

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
US6630301B1 (en) 1997-03-14 2003-10-07 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
WO1997034015A1 (en) 1996-03-15 1997-09-18 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
US20020132244A1 (en) 2000-09-27 2002-09-19 Xiao-Cheng Li-Sucholeiki Methods for detecting rare polymorphic variants in genomic DNA sequences
US6808884B2 (en) 2000-12-08 2004-10-26 California Institute Of Technology Methods and compositions for detecting polynucleotide duplex damage and errors
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
ATE548469T1 (de) 2005-10-14 2012-03-15 Univ State Cleveland Verfahren zur identifizierung mehrerer mutationen oder methylierungsmarkern in einem dominierenden hintergrund von wildtyp-dna
US20070154892A1 (en) 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids
ES2665500T3 (es) 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia
US20120164641A1 (en) 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
WO2013077282A1 (ja) 2011-11-25 2013-05-30 シャープ株式会社 液晶表示パネルの検査方法

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6120992A (en) * 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
US6031098A (en) * 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
WO2001005800A2 (en) * 1999-07-18 2001-01-25 Borries Kemper A method for the enrichment of heteroduplexes and its use in mutation detection
WO2007084380A2 (en) * 2006-01-13 2007-07-26 California Institute Of Technology Compounds and methods for nucleic acid mismatch detection

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US20140308664A1 (en) 2014-10-16
EP2935619A1 (en) 2015-10-28
WO2014096126A1 (en) 2014-06-26
CA2894212A1 (en) 2014-06-26
US9279146B2 (en) 2016-03-08
JP2016508713A (ja) 2016-03-24
CA2894212C (en) 2018-09-04
CN104903464A (zh) 2015-09-09
EP2935619B1 (en) 2017-09-06

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