CA2894212C - Compounds and methods comprising a rhodium-based intercalator for the enrichment of mutated nucleic acid from a mixture - Google Patents

Compounds and methods comprising a rhodium-based intercalator for the enrichment of mutated nucleic acid from a mixture Download PDF

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Publication number
CA2894212C
CA2894212C CA2894212A CA2894212A CA2894212C CA 2894212 C CA2894212 C CA 2894212C CA 2894212 A CA2894212 A CA 2894212A CA 2894212 A CA2894212 A CA 2894212A CA 2894212 C CA2894212 C CA 2894212C
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Canada
Prior art keywords
nucleic acid
allele
mismatch
variant
wild
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CA2894212A
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English (en)
French (fr)
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CA2894212A1 (en
Inventor
Amar Gupta
Nancy Schoenbrunner
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table
    • C07F15/0006—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic Table compounds of the platinum group
    • C07F15/0073—Rhodium compounds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pyridine Compounds (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Oxygen Or Sulfur (AREA)
CA2894212A 2012-12-21 2013-12-19 Compounds and methods comprising a rhodium-based intercalator for the enrichment of mutated nucleic acid from a mixture Active CA2894212C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261740895P 2012-12-21 2012-12-21
US61/740,895 2012-12-21
PCT/EP2013/077282 WO2014096126A1 (en) 2012-12-21 2013-12-19 Compounds and methods for the enrichment of mutated nucleic acid from a mixture

Publications (2)

Publication Number Publication Date
CA2894212A1 CA2894212A1 (en) 2014-06-26
CA2894212C true CA2894212C (en) 2018-09-04

Family

ID=49886901

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2894212A Active CA2894212C (en) 2012-12-21 2013-12-19 Compounds and methods comprising a rhodium-based intercalator for the enrichment of mutated nucleic acid from a mixture

Country Status (6)

Country Link
US (1) US9279146B2 (enExample)
EP (1) EP2935619B1 (enExample)
JP (1) JP2016508713A (enExample)
CN (1) CN104903464B (enExample)
CA (1) CA2894212C (enExample)
WO (1) WO2014096126A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3907297A1 (en) 2011-04-15 2021-11-10 The Johns Hopkins University Safe sequencing system
US11525163B2 (en) 2012-10-29 2022-12-13 The Johns Hopkins University Papanicolaou test for ovarian and endometrial cancers
US11286531B2 (en) 2015-08-11 2022-03-29 The Johns Hopkins University Assaying ovarian cyst fluid
SG11202001010UA (en) 2017-08-07 2020-03-30 Univ Johns Hopkins Methods and materials for assessing and treating cancer
EP3505643A1 (en) 2017-12-28 2019-07-03 Vito NV Methods for detecting nucleotide variants
CN108998504A (zh) * 2018-08-08 2018-12-14 鲍伟胜 一种提高基因突变检测灵敏度的方法
CA3170345A1 (en) 2020-02-14 2021-08-19 The Johns Hopkins University Methods and materials for assessing nucleic acids
GB2603203A (en) * 2021-02-02 2022-08-03 Univ Heriot Watt Method for enriching nucelic acids

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6027877A (en) * 1993-11-04 2000-02-22 Gene Check, Inc. Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, purification of amplified DNA samples and allele identification
US6120992A (en) 1993-11-04 2000-09-19 Valigene Corporation Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human
US6630301B1 (en) 1997-03-14 2003-10-07 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
WO1997034015A1 (en) 1996-03-15 1997-09-18 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum using nucleic acid amplification assays
US6031098A (en) 1997-08-11 2000-02-29 California Institute Of Technology Detection and treatment of duplex polynucleotide damage
SE9902741D0 (sv) 1999-07-19 1999-07-19 Boerries Kemper Method for the enrichment of heteroduplexes and its use in mutation detection
US20020132244A1 (en) 2000-09-27 2002-09-19 Xiao-Cheng Li-Sucholeiki Methods for detecting rare polymorphic variants in genomic DNA sequences
US6808884B2 (en) 2000-12-08 2004-10-26 California Institute Of Technology Methods and compositions for detecting polynucleotide duplex damage and errors
GB2382137A (en) 2001-11-20 2003-05-21 Mats Gullberg Nucleic acid enrichment
ATE548469T1 (de) 2005-10-14 2012-03-15 Univ State Cleveland Verfahren zur identifizierung mehrerer mutationen oder methylierungsmarkern in einem dominierenden hintergrund von wildtyp-dna
US20070154892A1 (en) 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids
WO2007084380A2 (en) 2006-01-13 2007-07-26 California Institute Of Technology Compounds and methods for nucleic acid mismatch detection
ES2665500T3 (es) 2010-03-08 2018-04-26 Dana-Farber Cancer Institute, Inc. Enriquecimiento de una PCR por COLD completa con secuencia de bloqueo de referencia
US20120164641A1 (en) 2010-12-22 2012-06-28 Roche Molecular Systems, Inc. Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene
WO2013077282A1 (ja) 2011-11-25 2013-05-30 シャープ株式会社 液晶表示パネルの検査方法

Also Published As

Publication number Publication date
US20140308664A1 (en) 2014-10-16
EP2935619A1 (en) 2015-10-28
WO2014096126A1 (en) 2014-06-26
CA2894212A1 (en) 2014-06-26
US9279146B2 (en) 2016-03-08
JP2016508713A (ja) 2016-03-24
CN104903464B (zh) 2018-04-24
CN104903464A (zh) 2015-09-09
EP2935619B1 (en) 2017-09-06

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Effective date: 20150608