CN104841266A - Microorganism-containing deodorant preparation method - Google Patents

Microorganism-containing deodorant preparation method Download PDF

Info

Publication number
CN104841266A
CN104841266A CN201410445960.9A CN201410445960A CN104841266A CN 104841266 A CN104841266 A CN 104841266A CN 201410445960 A CN201410445960 A CN 201410445960A CN 104841266 A CN104841266 A CN 104841266A
Authority
CN
China
Prior art keywords
eliminating smell
smell agent
preparation
cfu
weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201410445960.9A
Other languages
Chinese (zh)
Other versions
CN104841266B (en
Inventor
徐纯范
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Zhejiang Sansan Environmental Protection Technology Holding Group Co.,Ltd.
Original Assignee
GOD LIGHT DEVELOPMENT Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GOD LIGHT DEVELOPMENT Co Ltd filed Critical GOD LIGHT DEVELOPMENT Co Ltd
Priority to CN201410445960.9A priority Critical patent/CN104841266B/en
Publication of CN104841266A publication Critical patent/CN104841266A/en
Application granted granted Critical
Publication of CN104841266B publication Critical patent/CN104841266B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Landscapes

  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Disinfection, Sterilisation Or Deodorisation Of Air (AREA)

Abstract

The present invention relates to a microorganism-containing deodorant preparation method. The technical problem solved by the present invention is to provide a production method of a deodorant containing a variety of microorganisms and suitable for complex environments. The preparation method of the present invention is characterized by comprising: 1) taking fresh big-leaf mulberry leaf, washing, carrying out steam enzyme deactivating, and mincing to obtain a suspension; 2) taking sterile purified water, adding 18-20% by weight of the suspension, 7-9% by weight of corn dry powder, and 10-12% by weight of brown sugar, respectively adding 80000-100000 cfu/ml of Bacillus subtilis, 45000-50000 cfu/ml of Streptomyces microflavus, 80000-100000 cfu/ml of yeast, 60000-80000 cfu/ml of Aspergillus oryzae, and 80000-100000 cfu/ml of lactic acid bacteria, and uniformly stirring to obtain a base liquid; 3) fermenting; and 4) carrying out centrifugal separation on the fermented base liquid, and taking the supernatant to obtain the deodorant.

Description

A kind of preparation method of the eliminating smell agent containing microorganism
Technical field
The present invention relates to a kind of microorganism formulation, specifically a kind of preparation method of the eliminating smell agent containing microorganism, this eliminating smell agent is mainly used to remove paint-like flavour, and family's sewer peculiar smell.
Background technology
Along with socioeconomic development, the problem of environmental pollution of China is more and more serious, and particularly foul smell or toxic and harmful pollution problem become increasingly conspicuous.Foul gas is mainly derived from the places such as municipal sewage plant, refuse landfill, livestock and poultry farm.As one of the public hazards of environment, stench, except having an impact to the sense of smell of people, causes outside the offending sensations such as psychology detest.Also can cause the discomfort on health, as felt sick, having a headache, lose the appetite, sense of smell is lacked of proper care, insomnia, even emotional instability etc.Therefore, the improvement of odor pollution is become to the research focus of numerous scientific workers.
At present, the method of process foul gas has three major types, i.e. Physical (sheltering neutralization, dilution diffusion, absorption), chemical method (chemical washing, oxidation, burning, absorption), bioanalysis (filtration, absorption, compost etc.), most pronounced effects, the most friendly to environment, be microorganism treatment.The research work starting of China in microbial deodorant is more late, after the nineties in 20th century, just carry out relevant laboratory research work.Be applied in the aspects such as sewage disposal, garbage disposal, plant's harmless treatment at present, domestic rarely have the research biological taste removal technology being applied to paint more.Chinese patent literature CN101690735A discloses a kind of complex microorganism health-protection agent and preparation method thereof, is representative wherein.Its preparation method is, by weight percentage active Lactobacillus acidophilus kind 2.5% ~ 3.5%, maltose or brown sugar 3% ~ 4%, mineral water 92.5% ~ 94.5% are put into Plastic Drum, the fermented liquid obtained filters and namely can be used for peculiar smell in decontamination chamber (as paint smell) etc.But the bacterial classification of this health-care agent is too single, its product can not adapt to more complex environment, such as sewer peculiar smell.Inventor wish to develop a kind of have containing great Ye Chinese holly juice, the eliminating smell agent of multi-cultur es.
The great Ye Chinese holly system applicant Moraceae hybridization new plant that before five, six years, independent research is cultivated, it is strong positive plant, and rapidly, tolerance is strong in growth, and management easily, has the important function of improving the ecological environment, conserving water and soil concurrently, is applicable to artificial establishing in large scale.Great Ye Chinese holly plantation project is environmental protection project, does not relate to poisonous and harmful substance, do not need a large amount of water resources in planting process, and can dissolve excessive natural pond liquid and cultivation excreta.Once plant, gather in the crops 20 years, can gather in great Ye Chinese holly 5-6 time every year, the great Ye Chinese holly base of 100 mu produces out fresh stem and 1000 tons, blade per year.In view of its crude protein content is up to 20%-30%, be rich in amino acid, vitamin, carbohydrate, trace element, these fresh stems and blade can be processed into plant protein fodder raw material 200 tons, can fit in complete feed 667 tons, cultivating live pig 2000, and save food 100 tons.New plant great Ye Chinese holly emerges soon, awaits more extensively, more deep exploitation.At present, applicant builds great Ye Chinese holly Demonstration Base 3000 mu, fresh stem and blade public offering, and publication great Ye Chinese holly plant advertisement welcomes to introduce a fine variety.Great Ye Chinese holly water fermentation together with flora is standby knows liquid, is not reported so far.
Summary of the invention
The technical issues that need to address of the present invention are, provide a kind of production method that contain multiple-microorganism, that conform more complicated eliminating smell agent.
The preparation method of the eliminating smell agent containing microorganism of the present invention, according to following steps:
1) get the fresh blade of great Ye Chinese holly, cleaning, steam beating, rub, obtain suspension;
2) sterile purified water is got, add above-mentioned suspension 18-20%, corn dry powder 7-9%, brown sugar 10-12% by weight, add bacillus subtilis more respectively to 80000-100000cfu/ml, streptomyces microflavus to 45000-50000cfu/ml, yeast to 80000-100000cfu/ml, aspergillus oryzae to 60000-80000cfu/ml, lactic acid bacteria to 80000-100000cfu/ml, mix thoroughly, obtain base fluid;
3) be placed on shaking table by above-mentioned base fluid, at 35-40 DEG C, Anaerobic culturel is measured after 15 days day by day, stops fermentation when lactic acid bacteria clump count is greater than 1000000cfu/ml;
4) by fermented-base fluid centrifugation (general centrifuge speed is 180-220r/min, and centrifugation time is 2.5-3.5min), get supernatant, be eliminating smell agent (also claiming stoste).
Eliminating smell agent stoste can directly use, and as preferably, eliminating smell agent and dilution dilute according to the weight ratio of 1: 10 ~ 30, obtain use liquid, under the prerequisite ensureing effect, can reduce costs, facilitate filling use.Described dilution is pure water or the ethanol not higher than 20% mass concentration water-soluble (for keeping bacterial activity, avoiding using straight alcohol liquid or the high concentration ethanol aqueous solution).Stoste is diluted to variable concentrations, can to adapt to different peculiar smell, different environmental requirements.
According to service condition, in described eliminating smell agent, be added with the natural perfume material of appropriate amount, better to realize commercialization.
The biological raw material that the present invention relates to is novel and unique, and microorganism fungus kind is all common, conventional, high security and stability kind, also can not produce poisonous and harmful substance after cultivation.
The eliminating smell agent that the present invention obtains uses liquid, and for paint taste removal, recommend spray uses.Spray first time has been fitted up at paint, Direct spraying, in painted surface, afterwards every spray in 2 ~ 3 days once, continues to be no less than summer 3 times, spring and autumn 4 times, winter 5 times, not only permanently effectively can dispel sheet material and paint the peculiar smell distributed, paint finishes degree can also be increased.On probation through 30 example finishing paint, peculiar smell elimination effect is remarkable.Be used for deodorant for refregerator through 20 examples, spray with this product of converting stoste content 5 grams/cubic metre, reach expection deodorizing effect, food is not produced and pollute.
The eliminating smell agent that the present invention obtains uses liquid, for removing sewer peculiar smell, recommends thread to pour into.Pour stoste 200 milliliters through 15 examples into try out at tank, sewer pipe, tested respectively after 24 hours, at the lower mouth of a river with 1L/min traffic sampling 5min, H 2s concentration is from 1.5mg/m 3drop to 0.01mg/m 3, NH 3concentration is from 1mg/m 3drop to 0.15mg/m 3(being sample mean above), and have and necessarily prevent water-pipe blockage effect, has the original impeded drainages of 5 example logical, the unimpeded raising had in various degree of draining in 2-3 days after a procedure.
Compare with background technology, biological raw material of the present invention is novel and unique, and microorganism species compatibility is scientific and reasonable, and in microbial deodorant field accommodation more extensively, preparation method has more science, preciseness to product, is more suitable for large-scale production.
Detailed description of the invention
Below by embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1
1) get the fresh blade of great Ye Chinese holly, cleaning, steam beating, rub, obtain suspension;
2) sterile purified water 5KG is got, add above-mentioned suspension 18%, corn dry powder 9%, brown sugar 10% by weight percentage, add bacillus subtilis more respectively to 100000cfu/ml, streptomyces microflavus to 50000cfu/ml, yeast to 100000cfu/ml, aspergillus oryzae to 80000cfu/ml, lactic acid bacteria to 100000cfu/ml, mix thoroughly, obtain base fluid;
3) be placed on shaking table by above-mentioned base fluid, at 37 DEG C of temperature, Anaerobic culturel is measured after 15 days day by day, stops fermentation when lactic acid bacteria clump count is greater than 1000000cfu/ml;
4) by fermented-base fluid centrifuge with the centrifugation 3min of 200r/min, get supernatant, be eliminating smell agent (stoste).
5) by above-mentioned stoste according to 1: 10 weight portion dilute with water, obtain eliminating smell agent use liquid.
Embodiment 2
1) with embodiment 1;
2) sterile purified water 5KG is got, add above-mentioned suspension 19%, corn dry powder 8%, brown sugar 11% by weight, add bacillus subtilis more respectively to 90000cfu/ml, streptomyces microflavus to 50000cfu/ml, yeast to 80000cfu/ml, aspergillus oryzae to 80000cfu/ml, lactic acid bacteria to 90000cfu/ml, mix thoroughly, obtain base fluid;
3) with embodiment 1;
4) with embodiment 1;
5) by above-mentioned stoste according to 1: 30 weight portion with the ethanol water of 10% concentration dilution, obtain eliminating smell agent use liquid.
Embodiment 3
1) with embodiment 1;
2) sterile purified water 10KG is got, add above-mentioned suspension 20%, corn dry powder 7%, brown sugar 12% by weight, add bacillus subtilis more respectively to 90000cfu/ml, streptomyces microflavus to 50000cfu/ml, yeast to 90000cfu/ml, aspergillus oryzae to 780000cfu/ml, lactic acid bacteria to 100000cfu/ml, mix thoroughly, obtain base fluid;
3) with embodiment 1;
4) with embodiment 1;
5) by above-mentioned stoste according to 1: 20 weight portion with the ethanol water of 15% concentration dilution, obtain eliminating smell agent use liquid
Paint-removing taste statistical effect table is as follows:
Except sewer peculiar smell statistical effect table omits.

Claims (4)

1., containing a preparation method for the eliminating smell agent of microorganism, it is characterized in that according to following steps:
1) get the fresh blade of great Ye Chinese holly, cleaning, steam beating, rub, obtain suspension;
2) sterile purified water is got, add above-mentioned suspension 18-20%, corn dry powder 7-9%, brown sugar 10-12% by weight, add bacillus subtilis more respectively to 80000-100000cfu/ml, streptomyces microflavus to 45000-50000cfu/ml, yeast to 80000-100000cfu/ml, aspergillus oryzae to 60000-80000cfu/ml, lactic acid bacteria to 80000-100000cfu/ml, mix thoroughly, obtain base fluid;
3) be placed on shaking table by above-mentioned base fluid, at 35-40 DEG C, Anaerobic culturel is measured after 15 days day by day, stops fermentation when lactic acid bacteria clump count is greater than 1000000cfu/ml;
4) by fermented-base fluid centrifugation, get supernatant, be eliminating smell agent.
2. the preparation method of a kind of eliminating smell agent containing microorganism according to claim 1, is characterized in that eliminating smell agent agent and dilution dilute according to the weight ratio of 1:10 ~ 30.
3. the preparation method of a kind of eliminating smell agent containing microorganism according to claim 2, is characterized in that described dilution is pure water or not higher than the ethanol water of 20% mass concentration.
4. the preparation method of a kind of eliminating smell agent containing microorganism according to claim 1 or 2 or 3, is characterized in that the natural perfume material being added with appropriate amount in described eliminating smell agent.
CN201410445960.9A 2014-09-03 2014-09-03 A kind of preparation method of the eliminating smell agent containing microorganism Active CN104841266B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201410445960.9A CN104841266B (en) 2014-09-03 2014-09-03 A kind of preparation method of the eliminating smell agent containing microorganism

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201410445960.9A CN104841266B (en) 2014-09-03 2014-09-03 A kind of preparation method of the eliminating smell agent containing microorganism

Publications (2)

Publication Number Publication Date
CN104841266A true CN104841266A (en) 2015-08-19
CN104841266B CN104841266B (en) 2016-08-24

Family

ID=53841482

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201410445960.9A Active CN104841266B (en) 2014-09-03 2014-09-03 A kind of preparation method of the eliminating smell agent containing microorganism

Country Status (1)

Country Link
CN (1) CN104841266B (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106140800A (en) * 2015-04-02 2016-11-23 中环循(北京)环境技术中心 A kind of preparation method of environment-friendly type contaminated soil eliminating smell agent
CN106731775A (en) * 2017-01-22 2017-05-31 广州市佰好惠医疗科技有限公司 A kind of expansion type compound biological enzyme deodorant and preparation method and application
CN108853549A (en) * 2018-06-15 2018-11-23 黄少飞 A kind of ring draining and preparation method thereof for soot gas treatment
CN110090626A (en) * 2019-04-02 2019-08-06 杨为明 A kind of protein nano eliminating smell agent and preparation method thereof

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2810308B2 (en) * 1993-12-13 1998-10-15 旭化成工業株式会社 Deodorant containing microorganisms and their cells
CN1194798C (en) * 2002-03-19 2005-03-30 广东省微生物研究所 Biologic deodorization method and its device
CN101182479A (en) * 2007-11-05 2008-05-21 上海创博生态工程有限公司 Microorganism cultured birds padding additive and method for making same
CN101503664A (en) * 2009-03-12 2009-08-12 沈阳惠泽生物制品有限公司 Method for producing efficient composite microbial preparation by livestock and poultry liquid dung
CN101638626B (en) * 2009-09-10 2011-04-20 惠飞 Bacillus subtilis and application thereof in biological deodorant
CN101690735A (en) * 2009-09-16 2010-04-07 周汉华 Complex microorganism health-protection agent and preparation method thereof
KR101296613B1 (en) * 2012-10-10 2013-08-14 썬앤문바이오 주식회사 A microbe deodorizer
CN103923849A (en) * 2013-01-10 2014-07-16 都江堰惠农生物技术有限责任公司 Production method of compound bacteria for removing stink from livestock and poultry culturing farm
CN103642719B (en) * 2013-11-26 2016-01-20 北京市京圃园生物工程有限公司 A kind of complex microbial inoculum and the application in adjustment soil acidity or alkalinity thereof
CN103865847B (en) * 2014-03-06 2016-06-08 深圳市三林生物科技工程有限公司 For complex microorganism preparations and its preparation method of rubbish deodorizing

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106140800A (en) * 2015-04-02 2016-11-23 中环循(北京)环境技术中心 A kind of preparation method of environment-friendly type contaminated soil eliminating smell agent
CN106731775A (en) * 2017-01-22 2017-05-31 广州市佰好惠医疗科技有限公司 A kind of expansion type compound biological enzyme deodorant and preparation method and application
CN108853549A (en) * 2018-06-15 2018-11-23 黄少飞 A kind of ring draining and preparation method thereof for soot gas treatment
CN110090626A (en) * 2019-04-02 2019-08-06 杨为明 A kind of protein nano eliminating smell agent and preparation method thereof

Also Published As

Publication number Publication date
CN104841266B (en) 2016-08-24

Similar Documents

Publication Publication Date Title
CN101130101B (en) Compound deodorizer of microorganism and polyporous material and method of producing the same
CN104894007B (en) Complex microorganism preparations and preparation method and the application in processing animal carcass
CN108048344B (en) Two plants of deodorization bacterial strains and its application in preparation composite biological deodorant
CN107189972A (en) Microbial deodorant, preparation method and the usage
CN105536509A (en) Plant extract and microbe composite deodorant
CN101791421A (en) Microorganism cleaning bactericide capable of removing multiple poisonous and harmful substances
CN103977704B (en) A method of deodorization being carried out to rubbish using complex micro organism fungicide
CN108949739A (en) A kind of complex micro organism fungicide and preparation method thereof for advanced treating high concentration livestock breeding wastewater
KR101989280B1 (en) Deodorant and manufacturing method thereof
CN105435279B (en) A kind of compound deodorizer coordinates the deodorizing method of washing deodorizing tower
CN102813948A (en) Preparation method of composite biological deodorant
CN104841266A (en) Microorganism-containing deodorant preparation method
CN109092049A (en) A kind of biologic ferment deodorant
CN105642105A (en) Intelligent biological deodorizer
CN106282063A (en) One bacillus amyloliquefaciens bacterial strain and the application in rubbish deodorization thereof
CN105670964A (en) Bacillus atrophaeus strain BsR05 and application thereof
CN106982861A (en) A kind of biologic garbage bacteria remover and preparation method thereof
CN101603012A (en) A kind of Biological fitler method de-odorised microbial inoculum and preparation thereof, biofilm inoculation method of being used for
JP6111386B2 (en) Deodorant manufacturing method
KR101450019B1 (en) Cattle's wastes-manure
CN108619906A (en) Environmentally friendly finishing eliminating smell agent and preparation method thereof
CN108531412A (en) A kind of preparation method of ECMD Efficient Rings control microbial deodorant
CN107446866A (en) A kind of dirty complex micro organism fungicide of dystopy fermentation process excrement
CN104120100A (en) Endogenous bacillus megatherium and applications thereof in restoration of quinclorac phytotoxicity
CN103740613A (en) Preparation method for biological active bacterium agent

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
EXSB Decision made by sipo to initiate substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
CB03 Change of inventor or designer information

Inventor after: Xu Biao

Inventor after: Xu Chunfan

Inventor before: Xu Chunfan

COR Change of bibliographic data
TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20180305

Address after: Wan Xing Lu Wan Tian Xiang Kecheng District 324000 Zhejiang city of Quzhou province No. 1 Room 302

Patentee after: Zhejiang yuan Lai Biology Technology Co., Ltd.

Address before: 3 floor, block B, zeshhui City, Xu Jiawu, xujiawu, Quzhou City, Zhejiang

Patentee before: God Light Development Co., Ltd.

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20181127

Address after: 324000 Haili Avenue, Qujiang District, Quzhou City, Zhejiang Province

Patentee after: Yan Ling

Address before: Room 302, No. 1 Wanxing Road, Wantian Township, Kecheng District, Quzhou City, Zhejiang Province

Patentee before: Zhejiang yuan Lai Biology Technology Co., Ltd.

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20191210

Address after: No. 94, shunduibian village, Wantian Township, Kecheng District, Quzhou City, Zhejiang Province

Patentee after: Chen Caixian

Address before: 324000 Haili Avenue, Qujiang District, Quzhou City, Zhejiang Province

Patentee before: Yan Ling

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20200120

Address after: 324000 Haili Avenue, Qujiang District, Quzhou City, Zhejiang Province

Patentee after: Yan Ling

Address before: No. 94, shunduibian village, Wantian Township, Kecheng District, Quzhou City, Zhejiang Province

Patentee before: Chen Caixian

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20210422

Address after: Room 3001, 3rd floor, No.18 Shilongshan industrial block, Zhuantang street, Xihu District, Hangzhou City, Zhejiang Province, 310013

Patentee after: Zhejiang Lingyu Wukang Technology Group Co.,Ltd.

Address before: 324000 Haili Avenue, Qujiang District, Quzhou City, Zhejiang Province

Patentee before: Yan Ling

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20211222

Address after: 310013 room 902-1, building 1, binlan Mingzuo, Shuangpu Town, Xihu District, Hangzhou City, Zhejiang Province

Patentee after: Zhejiang Sansan Environmental Protection Technology Holding Group Co.,Ltd.

Address before: Room 3001, 3rd floor, No.18 Shilongshan industrial block, Zhuantang street, Xihu District, Hangzhou City, Zhejiang Province, 310013

Patentee before: Zhejiang Lingyu Wukang Technology Group Co.,Ltd.