CN104841266B - A kind of preparation method of the eliminating smell agent containing microorganism - Google Patents

A kind of preparation method of the eliminating smell agent containing microorganism Download PDF

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CN104841266B
CN104841266B CN201410445960.9A CN201410445960A CN104841266B CN 104841266 B CN104841266 B CN 104841266B CN 201410445960 A CN201410445960 A CN 201410445960A CN 104841266 B CN104841266 B CN 104841266B
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eliminating smell
smell agent
microorganism
100000cfu
base fluid
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CN104841266A (en
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徐纯范
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Zhejiang Lingyu Wukang Technology Group Co.,Ltd.
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God Light Development Co ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/20Air quality improvement or preservation, e.g. vehicle emission control or emission reduction by using catalytic converters

Abstract

The present invention relates to the preparation method of a kind of eliminating smell agent containing microorganism, the technical issues that need to address are to provide a kind of containing multiple-microorganism, the production method that adapts to the more complicated eliminating smell agent of environment.The preparation method of the present invention, it is characterised in that according to following steps: 1) take the fresh blade of large-leaf fructus lycii healthcare, clean, steam beating, rub, obtain suspension;2) sterile purified water is taken, add above-mentioned suspension 18 20%, Semen Maydis dry powder 7 9%, brown sugar 10 12% by weight, it is separately added into bacillus subtilis again to 80000 100000cfu/ml, streptomyces microflavus to 45000 50000cfu/ml, yeast to 80000 100000cfu/ml, aspergillus oryzae to 60000 80000cfu/ml, lactic acid bacteria to 80000 100000cfu/ml, mix thoroughly, obtain base fluid;3) fermentation;4) the base fluid centrifugation fermented, takes supernatant and i.e. obtains eliminating smell agent.

Description

A kind of preparation method of the eliminating smell agent containing microorganism
Technical field
The present invention relates to a kind of microorganism formulation, the preparation method of a kind of eliminating smell agent containing microorganism, This eliminating smell agent is mainly used to remove paint-like flavour, and family's sewer abnormal flavour.
Background technology
Along with socioeconomic development, the problem of environmental pollution of China is increasingly severe, the most smelly Gas or toxic and harmful pollution problem become increasingly conspicuous.Foul gas is mainly derived from municipal sewage treatment The places such as factory, refuse landfill, livestock and poultry farm.One of public hazards as environment, stench is except to people Olfactory sensation produce impact, cause outside the psychology offending sensation such as detest.Also can cause the discomfort on health, Such as nauseating, headache, inappetence, olfactory sensation imbalance, insomnia, even emotional instability etc..Therefore, right The improvement of odor pollution becomes the research focus of numerous scientific workers.
At present, process foul gas method have three major types, i.e. Physical (shelter neutralizations, dilution spread, Absorption), chemical method (chemical washing, aoxidize, burn, absorb), bioanalysis (filter, absorb, compost etc.), Most pronounced effects, the most friendly to environment, it is microorganism treatment.China is in terms of microbial deodorant Research work starting ratio is later, just carries out relevant laboratory research work after the nineties in 20th century. Apply at aspects such as sewage disposal, garbage disposal, plant's harmless treatments at present more, domestic rarely have by Biological taste removal technology is applied to the research of paint.Chinese patent literature CN101690735A discloses one Complex microorganism health-protection agent and preparation method thereof, is representative therein.Its preparation method is, by weight Percentage ratio is by activity Lactobacillus Acidophilus kind 2.5%~3.5%, maltose or brown sugar 3%~4%, mineral water 92.5%~94.5% puts into Plastic Drum, and the fermented liquid obtained filters and i.e. can be used for abnormal flavour in decontamination chamber (such as paint abnormal smells from the patient) etc..But the strain of this health-care agent is the most single, its product does not adapt to more complicated Environment, such as sewer abnormal flavour.Inventor wishes to develop a kind of to be had containing large-leaf fructus lycii healthcare juice, multi-cultur es Eliminating smell agent.
The large-leaf fructus lycii healthcare system applicant Moraceae that before five, six years, independent research is cultivated hybridization new plant, it is Strong positive plant, rapidly, toleration is strong in growth, and management easily, has concurrently and improves the ecological environment, keeps water The important function of soil, is suitable for artificial establishing in large scale.Large-leaf fructus lycii healthcare plantation project is environmental protection project, plants Poisonous and harmful substance it is not related to, it is not necessary to substantial amounts of water resource during planting, and excessive natural pond of can dissolving Liquid and cultivation Excreta.Once plant, gather in the crops 20 years, large-leaf fructus lycii healthcare can be gathered in 5-6 time every year, one 100 The large-leaf fructus lycii healthcare base of mu produces out fresh stem and 1000 tons of blade per year.In view of its crude protein content up to 20%-30%, rich in aminoacid, vitamin, carbohydrate, trace element, these fresh stems and Blade can be processed into plant protein fodder raw material 200 tons, can fit in complete feed 667 tons, and cultivation is raw Pig 2000, and save food 100 tons.New plant large-leaf fructus lycii healthcare emerges soon, awaits more extensively, more Deep exploitation.At present, applicant builds large-leaf fructus lycii healthcare Demonstration Base 3000 mu, fresh stem and blade Disclosed sale, publication large-leaf fructus lycii healthcare plant advertisement welcomes to introduce a fine variety.Large-leaf fructus lycii healthcare water rises with flora one Ferment preparation understands liquid, is not reported so far.
Summary of the invention
The technical issues that need to address of the present invention be to provide a kind of containing multiple-microorganism, adapt to environment more The production method of complicated eliminating smell agent.
The preparation method of the eliminating smell agent containing microorganism of the present invention, according to following steps:
1) take the fresh blade of large-leaf fructus lycii healthcare, clean, steam beating, rub, obtain suspension;
2) take sterile purified water, add by weight above-mentioned suspension 18-20%, Semen Maydis dry powder 7-9%, Brown sugar 10-12%, then it is separately added into bacillus subtilis to 80000-100000cfu/ml, streptomyces microflavus Arrive to 45000-50000cfu/ml, yeast to 80000-100000cfu/ml, aspergillus oryzae 60000-80000cfu/ml, lactic acid bacteria, to 80000-100000cfu/ml, are mixed thoroughly, obtain base fluid;
3) being placed on shaking table by above-mentioned base fluid, at 35-40 DEG C, Anaerobic culturel is measured after 15 days day by day, when Lactic acid bacteria clump count is more than stopping fermentation during 1000000cfu/ml;
4) by fermented-base fluid centrifugation, (general centrifuge speed is 180-220r/min, time centrifugal Between be 2.5-3.5min), take supernatant, be eliminating smell agent (also referred to as stock solution).
Eliminating smell agent stock solution can directly use, as preferably, eliminating smell agent and diluent according to 1: 10~30 weight Amount ratio is diluted, and obtains use liquid, can reduce cost, facilitate fill to make on the premise of ensureing effect With.Described diluent is that the ethanol of pure water or not higher than 20% mass concentration is water-soluble (for keeping flora to live Property, it is to avoid use straight alcohol liquid or high concentration ethanol aqueous solution).Stock solution is diluted to variable concentrations, can be with Adapt to different abnormal flavour, different environmental requirements.
According to service condition, described eliminating smell agent is added with proper amount of natural perfume material, with the most real Existing commercialization.
The biological raw material that the present invention relates to is novel and unique, and microorganism fungus kind is all common, conventional, Gao An Full property and stability kind, also will not produce poisonous and harmful substance after cultivation.
The eliminating smell agent that the present invention obtains uses liquid, for paint taste removal, it is recommended that spraying uses.At paint dress Having repaiied spray for the first time, Direct spraying, in painted surface, afterwards every spray in 2~3 days once, continues not Less than summer 3 times, spring and autumn 4 times, winter 5 times, sheet material and paint not only permanently effective can be dispelled The abnormal flavour distributed, it is also possible to increase paint finishes degree.On probation through 30 example finishing paint, abnormal flavour elimination effect Significantly.Through 20 examples for deodorant for refregerator, spray converting this product of stock solution content 5 gram/cubic metre, Reach expection deodorizing effect, food is produced and pollute.
The eliminating smell agent that the present invention obtains uses liquid, for removing sewer abnormal flavour, it is recommended that thread is poured into.Warp 15 examples are poured stock solution 200 milliliters into try out at tank, sewer pipes, test respectively after 24 hours, The lower mouth of a river is with 1L/min traffic sampling 5min, H2S concentration is from 1.5mg/m3Drop to 0.01mg/m3, NH3 Concentration is from 1mg/m3Drop to 0.15mg/m3(being sample mean above), and have certain anti-antipriming pipe to block up Plug effect, has the 5 original impeded drainages of example to lead to, and draining in 2-3 days is unimpeded after a procedure has in various degree Improve.
Compared with background technology, biological raw material of the present invention is novel and unique, the conjunction of microorganism species compatibility science Reason, product is wider at microbial deodorant field subject range, and preparation method is more scientific, preciseness, It is more suitable for large-scale production.
Detailed description of the invention
Below by embodiment, technical scheme is described in further detail.
Embodiment 1
1) take the fresh blade of large-leaf fructus lycii healthcare, clean, steam beating, rub, obtain suspension;
2) take sterile purified water 5KG, add above-mentioned suspension 18%, Semen Maydis dry powder by weight percentage 9%, brown sugar 10%, then be separately added into bacillus subtilis and arrive to 100000cfu/ml, streptomyces microflavus 50000cfu/ml, yeast arrive to 100000cfu/ml, aspergillus oryzae to 80000cfu/ml, lactic acid bacteria 100000cfu/ml, mixes thoroughly, obtains base fluid;
3) being placed on shaking table by above-mentioned base fluid, at a temperature of 37 DEG C, Anaerobic culturel is measured after 15 days day by day, Fermentation is stopped when lactic acid bacteria clump count is more than 1000000cfu/ml;
4) by fermented-base fluid centrifuge with the centrifugation 3min of 200r/min, supernatant is taken, For eliminating smell agent (stock solution).
5) above-mentioned stock solution is diluted with water according to the weight portion of 1: 10, obtain eliminating smell agent and use liquid.
Embodiment 2
1) with embodiment 1;
2) take sterile purified water 5KG, add by weight above-mentioned suspension 19%, Semen Maydis dry powder 8%, Brown sugar 11%, then be separately added into bacillus subtilis and arrive to 90000cfu/ml, streptomyces microflavus 50000cfu/ml, yeast arrive to 80000cfu/ml, aspergillus oryzae to 80000cfu/ml, lactic acid bacteria 90000cfu/ml, mixes thoroughly, obtains base fluid;
3) with embodiment 1;
4) with embodiment 1;
5) above-mentioned stock solution is diluted with the ethanol water of 10% concentration according to the weight portion of 1: 30, to obtain final product Eliminating smell agent uses liquid.
Embodiment 3
1) with embodiment 1;
2) take sterile purified water 10KG, add by weight above-mentioned suspension 20%, Semen Maydis dry powder 7%, Brown sugar 12%, then be separately added into bacillus subtilis and arrive to 90000cfu/ml, streptomyces microflavus 50000cfu/ml, yeast arrive to 90000cfu/ml, aspergillus oryzae to 780000cfu/ml, lactic acid bacteria 100000cfu/ml, mixes thoroughly, obtains base fluid;
3) with embodiment 1;
4) with embodiment 1;
5) above-mentioned stock solution is diluted with the ethanol water of 15% concentration according to the weight portion of 1: 20, to obtain final product Eliminating smell agent uses liquid
Paint-removing taste statistical effect table is as follows:
Except sewer abnormal flavour statistical effect table slightly.

Claims (4)

1. the preparation method of the eliminating smell agent containing microorganism, it is characterised in that according to following steps:
1) take the fresh blade of large-leaf fructus lycii healthcare, clean, steam beating, rub, obtain suspension;
2) sterile purified water is taken, add above-mentioned suspension 18-20%, Semen Maydis dry powder 7-9%, brown sugar 10-12% by weight, it is separately added into bacillus subtilis again to 80000-100000cfu/ml, streptomyces microflavus to 45000-50000cfu/ml, yeast to 80000-100000cfu/ml, aspergillus oryzae to 60000-80000cfu/ml, lactic acid bacteria to 80000-100000cfu/ml, mix thoroughly, obtain base fluid;
3) being placed on shaking table by above-mentioned base fluid, at 35-40 DEG C, Anaerobic culturel is measured after 15 days day by day, stops fermentation when lactic acid bacteria clump count is more than 1000000cfu/ml;
4) by fermented-base fluid centrifugation, take supernatant, be eliminating smell agent.
The preparation method of a kind of eliminating smell agent containing microorganism the most according to claim 1, it is characterised in that eliminating smell agent and diluent are diluted according to the weight ratio of 1:10 ~ 30.
The preparation method of a kind of eliminating smell agent containing microorganism the most according to claim 2, it is characterised in that described diluent is pure water or the ethanol water of not higher than 20% mass concentration.
4. according to the preparation method of a kind of eliminating smell agent containing microorganism described in claim 1 or 2 or 3, it is characterised in that described eliminating smell agent is added with proper amount of natural perfume material.
CN201410445960.9A 2014-09-03 2014-09-03 A kind of preparation method of the eliminating smell agent containing microorganism Active CN104841266B (en)

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CN106140800A (en) * 2015-04-02 2016-11-23 中环循(北京)环境技术中心 A kind of preparation method of environment-friendly type contaminated soil eliminating smell agent
CN106731775A (en) * 2017-01-22 2017-05-31 广州市佰好惠医疗科技有限公司 A kind of expansion type compound biological enzyme deodorant and preparation method and application
CN108853549A (en) * 2018-06-15 2018-11-23 黄少飞 A kind of ring draining and preparation method thereof for soot gas treatment
CN110090626A (en) * 2019-04-02 2019-08-06 杨为明 A kind of protein nano eliminating smell agent and preparation method thereof

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Inventor after: Xu Biao

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