CN104830688B - 一种微生物芽孢体干悬浮剂的制备方法 - Google Patents
一种微生物芽孢体干悬浮剂的制备方法 Download PDFInfo
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- CN104830688B CN104830688B CN201510270651.7A CN201510270651A CN104830688B CN 104830688 B CN104830688 B CN 104830688B CN 201510270651 A CN201510270651 A CN 201510270651A CN 104830688 B CN104830688 B CN 104830688B
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- Prior art keywords
- microbial spores
- body fluid
- carrier
- preparation
- suspending agent
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 68
- 238000002360 preparation method Methods 0.000 title claims abstract description 39
- 239000000375 suspending agent Substances 0.000 title claims abstract description 32
- 210000005056 cell body Anatomy 0.000 title claims abstract description 13
- 210000001124 body fluid Anatomy 0.000 claims abstract description 42
- 239000010839 body fluid Substances 0.000 claims abstract description 42
- 238000000855 fermentation Methods 0.000 claims abstract description 29
- 230000004151 fermentation Effects 0.000 claims abstract description 29
- 238000000034 method Methods 0.000 claims abstract description 25
- 238000005469 granulation Methods 0.000 claims abstract description 16
- 230000003179 granulation Effects 0.000 claims abstract description 16
- 238000002156 mixing Methods 0.000 claims abstract description 16
- 239000000203 mixture Substances 0.000 claims abstract description 13
- 238000005507 spraying Methods 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 25
- 239000002270 dispersing agent Substances 0.000 claims description 9
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 7
- 238000007664 blowing Methods 0.000 claims description 6
- 239000013049 sediment Substances 0.000 claims description 6
- 239000000080 wetting agent Substances 0.000 claims description 6
- 239000000843 powder Substances 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- 239000003223 protective agent Substances 0.000 claims description 4
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims description 3
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 3
- RCEAADKTGXTDOA-UHFFFAOYSA-N OS(O)(=O)=O.CCCCCCCCCCCC[Na] Chemical group OS(O)(=O)=O.CCCCCCCCCCCC[Na] RCEAADKTGXTDOA-UHFFFAOYSA-N 0.000 claims description 3
- 239000004372 Polyvinyl alcohol Substances 0.000 claims description 3
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims description 3
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 3
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims description 3
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 229920002451 polyvinyl alcohol Polymers 0.000 claims description 3
- 239000001632 sodium acetate Substances 0.000 claims description 3
- 235000017281 sodium acetate Nutrition 0.000 claims description 3
- LGQKYAXCIDXXEG-UHFFFAOYSA-L barium(2+);dodecyl sulfate Chemical compound [Ba+2].CCCCCCCCCCCCOS([O-])(=O)=O.CCCCCCCCCCCCOS([O-])(=O)=O LGQKYAXCIDXXEG-UHFFFAOYSA-L 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical group [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 2
- 238000001035 drying Methods 0.000 abstract description 9
- 241000894006 Bacteria Species 0.000 abstract description 8
- 238000012545 processing Methods 0.000 abstract description 7
- 230000000694 effects Effects 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 239000002131 composite material Substances 0.000 abstract description 2
- 241000194108 Bacillus licheniformis Species 0.000 description 14
- 239000000047 product Substances 0.000 description 12
- 244000005700 microbiome Species 0.000 description 5
- 241000726221 Gemma Species 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 2
- 235000014469 Bacillus subtilis Nutrition 0.000 description 2
- RZYKUPXRYIOEME-UHFFFAOYSA-N CCCCCCCCCCCC[S] Chemical compound CCCCCCCCCCCC[S] RZYKUPXRYIOEME-UHFFFAOYSA-N 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 2
- -1 alkyl sodium salt Chemical class 0.000 description 2
- 239000007859 condensation product Substances 0.000 description 2
- 229940051841 polyoxyethylene ether Drugs 0.000 description 2
- 229920000056 polyoxyethylene ether Polymers 0.000 description 2
- 238000012805 post-processing Methods 0.000 description 2
- 238000003672 processing method Methods 0.000 description 2
- GEHJYWRUCIMESM-UHFFFAOYSA-L sodium sulfite Chemical compound [Na+].[Na+].[O-]S([O-])=O GEHJYWRUCIMESM-UHFFFAOYSA-L 0.000 description 2
- DMAXMXPDVWTIRV-UHFFFAOYSA-N 2-(2-phenylethyl)phenol Chemical compound OC1=CC=CC=C1CCC1=CC=CC=C1 DMAXMXPDVWTIRV-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 229920001732 Lignosulfonate Polymers 0.000 description 1
- SUZRRICLUFMAQD-UHFFFAOYSA-N N-Methyltaurine Chemical class CNCCS(O)(=O)=O SUZRRICLUFMAQD-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001335 aliphatic alkanes Chemical class 0.000 description 1
- 125000002877 alkyl aryl group Chemical group 0.000 description 1
- 150000008052 alkyl sulfonates Chemical class 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 229910052788 barium Inorganic materials 0.000 description 1
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 229940118056 cresol / formaldehyde Drugs 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 150000002191 fatty alcohols Chemical class 0.000 description 1
- 238000007667 floating Methods 0.000 description 1
- NVVZQXQBYZPMLJ-UHFFFAOYSA-N formaldehyde;naphthalene-1-sulfonic acid Chemical compound O=C.C1=CC=C2C(S(=O)(=O)O)=CC=CC2=C1 NVVZQXQBYZPMLJ-UHFFFAOYSA-N 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 239000000825 pharmaceutical preparation Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 229920005646 polycarboxylate Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 235000010265 sodium sulphite Nutrition 0.000 description 1
- 150000005846 sugar alcohols Polymers 0.000 description 1
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 239000004563 wettable powder Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/04—Preserving or maintaining viable microorganisms
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
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CN201510270651.7A CN104830688B (zh) | 2015-05-18 | 2015-05-18 | 一种微生物芽孢体干悬浮剂的制备方法 |
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CN201510270651.7A CN104830688B (zh) | 2015-05-18 | 2015-05-18 | 一种微生物芽孢体干悬浮剂的制备方法 |
Publications (2)
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CN104830688A CN104830688A (zh) | 2015-08-12 |
CN104830688B true CN104830688B (zh) | 2018-11-27 |
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Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104000025A (zh) * | 2014-06-11 | 2014-08-27 | 北京昕地美饲料科技有限公司 | 一种稳定化乳酸菌产品的制备方法 |
CN104232531A (zh) * | 2014-08-29 | 2014-12-24 | 湖北省生物农药工程研究中心 | 一种芽孢杆菌低温干燥制粒工艺 |
CN104522054A (zh) * | 2015-01-09 | 2015-04-22 | 馥稷生物科技发展(上海)有限公司 | 一种生物源增效复配杀虫剂、制备方法及其应用 |
-
2015
- 2015-05-18 CN CN201510270651.7A patent/CN104830688B/zh active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104000025A (zh) * | 2014-06-11 | 2014-08-27 | 北京昕地美饲料科技有限公司 | 一种稳定化乳酸菌产品的制备方法 |
CN104232531A (zh) * | 2014-08-29 | 2014-12-24 | 湖北省生物农药工程研究中心 | 一种芽孢杆菌低温干燥制粒工艺 |
CN104522054A (zh) * | 2015-01-09 | 2015-04-22 | 馥稷生物科技发展(上海)有限公司 | 一种生物源增效复配杀虫剂、制备方法及其应用 |
Non-Patent Citations (1)
Title |
---|
真空后喷涂技术在颗粒饲料中添加微生态制剂的应用;董颖超 等;《中国饲料》;20080220(第4期);第37-38,41页 * |
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CN104830688A (zh) | 2015-08-12 |
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Addressee: NINGBO JIANGDONG HUIZE BIOTECHNOLOGY CO.,LTD. Document name: Notification of an Office Action |
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Address after: 315000 365 Zhen Zhen Road, Yinzhou District, Ningbo, Zhejiang Applicant after: NINGBO HUIZE BIOTECHNOLOGY CO.,LTD. Address before: 315000 No. 10 (33) (18-1) - (18-6) 18100 room, new world East District, Jiangdong District, Ningbo, Zhejiang. Applicant before: NINGBO JIANGDONG HUIZE BIOTECHNOLOGY CO.,LTD. |
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PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Preparation method of microbial brood-gemma dry suspending agent Effective date of registration: 20190329 Granted publication date: 20181127 Pledgee: Ningbo Tianjin Enterprise Service Co.,Ltd. Pledgor: NINGBO HUIZE BIOTECHNOLOGY CO.,LTD. Registration number: 2019330000086 |
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Effective date of registration: 20210910 Address after: 314500 room 2206-22, floor 22, building 3, No. 1156, Gaoqiao Street (Development Zone), Tongxiang City, Jiaxing City, Zhejiang Province Patentee after: Zhongke Huihe Biotechnology (Jiaxing) Co.,Ltd. Address before: 315000 365 Zhen Zhen Road, Yinzhou District, Ningbo, Zhejiang Patentee before: NINGBO HUIZE BIOTECHNOLOGY Co.,Ltd. |
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Effective date of registration: 20221228 Address after: 315000 365 Zhen Zhen Road, Yinzhou District, Ningbo, Zhejiang Patentee after: NINGBO HUIZE BIOTECHNOLOGY CO.,LTD. Address before: 314500 room 2206-22, floor 22, building 3, No. 1156, Gaoqiao Street (Development Zone), Tongxiang City, Jiaxing City, Zhejiang Province Patentee before: Zhongke Huihe Biotechnology (Jiaxing) Co.,Ltd. |
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