CN104807692B - 肽的回收方法、肽的检测方法及试剂 - Google Patents

肽的回收方法、肽的检测方法及试剂 Download PDF

Info

Publication number
CN104807692B
CN104807692B CN201510033697.7A CN201510033697A CN104807692B CN 104807692 B CN104807692 B CN 104807692B CN 201510033697 A CN201510033697 A CN 201510033697A CN 104807692 B CN104807692 B CN 104807692B
Authority
CN
China
Prior art keywords
peptide
albumin
liquid sample
blood
recovery rate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201510033697.7A
Other languages
English (en)
Chinese (zh)
Other versions
CN104807692A (zh
Inventor
岩井敦史
津国早苗
川崎佳奈
加畑博幸
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sysmex Corp
Original Assignee
Sysmex Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sysmex Corp filed Critical Sysmex Corp
Publication of CN104807692A publication Critical patent/CN104807692A/zh
Application granted granted Critical
Publication of CN104807692B publication Critical patent/CN104807692B/zh
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/34Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/145Extraction; Separation; Purification by extraction or solubilisation
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416Systems
    • G01N27/447Systems using electrophoresis
    • G01N27/44704Details; Accessories
    • G01N27/44747Composition of gel or of carrier mixture

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Dispersion Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Electrochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Sampling And Sample Adjustment (AREA)
CN201510033697.7A 2014-01-29 2015-01-23 肽的回收方法、肽的检测方法及试剂 Expired - Fee Related CN104807692B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2014014517A JP6182465B2 (ja) 2014-01-29 2014-01-29 ペプチドの回収方法
JP2014-014517 2014-01-29

Publications (2)

Publication Number Publication Date
CN104807692A CN104807692A (zh) 2015-07-29
CN104807692B true CN104807692B (zh) 2020-11-03

Family

ID=52434602

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201510033697.7A Expired - Fee Related CN104807692B (zh) 2014-01-29 2015-01-23 肽的回收方法、肽的检测方法及试剂

Country Status (4)

Country Link
US (1) US9624263B2 (enExample)
EP (1) EP2902408B1 (enExample)
JP (1) JP6182465B2 (enExample)
CN (1) CN104807692B (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6675167B2 (ja) 2015-08-28 2020-04-01 シスメックス株式会社 ペプチドの遊離方法及び回収方法、並びにペプチド遊離剤及び試薬キット
JP6979797B2 (ja) 2017-05-31 2021-12-15 シスメックス株式会社 固液分離方法、固液分離装置およびそれに用いるピペットチップ、粒子およびキット

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5632421A (en) * 1979-08-27 1981-04-01 Green Cross Corp:The Fractionation of plasma protein
JP4644604B2 (ja) * 2004-01-20 2011-03-02 一般財団法人化学及血清療法研究所 2価陽イオン存在下加熱処理によるヒト血清アルブミンの製造方法
KR20100053529A (ko) * 2007-08-08 2010-05-20 스쿨 주리디칼 퍼슨 키타사토 인스티튜트 체액 시료에서의 저분자량 단백질 및 펩티드의 농축 방법
JP5636331B2 (ja) * 2011-04-28 2014-12-03 シスメックス株式会社 ペプチドの遊離方法および回収方法

Also Published As

Publication number Publication date
EP2902408B1 (en) 2016-04-06
US20150210736A1 (en) 2015-07-30
JP2015140320A (ja) 2015-08-03
JP6182465B2 (ja) 2017-08-16
US9624263B2 (en) 2017-04-18
CN104807692A (zh) 2015-07-29
EP2902408A1 (en) 2015-08-05

Similar Documents

Publication Publication Date Title
JPWO2016194114A1 (ja) モノクローナル抗体の定量方法
JPS644621B2 (enExample)
CN104807692B (zh) 肽的回收方法、肽的检测方法及试剂
JPWO2011092796A1 (ja) アミロイドβ測定方法
EP2902407B1 (en) Method of recovering peptide
JPWO2011071155A1 (ja) 塩基性ペプチドの検出方法および塩基性ペプチド検出用試薬
US8716440B2 (en) Method of liberating and recovering peptide
JP5857312B2 (ja) 心疾患診断マーカー
JPWO2016143226A1 (ja) 反応場を制限したプロテアーゼ分解反応による抗体からペプチド断片を得る方法
JP6473366B2 (ja) 温度判定方法、標的ペプチドの検出方法および温度判定試薬
JP6675167B2 (ja) ペプチドの遊離方法及び回収方法、並びにペプチド遊離剤及び試薬キット
JP7203407B2 (ja) 質量分析用のペプチド試料の調製方法
JP5717658B2 (ja) 副腎皮質刺激ホルモンの検出方法および吸着剤
CN103884569B (zh) 水牛卵泡液多肽组的制备和质谱分析方法
JP4284135B2 (ja) タンパク質分析方法
JP2007139759A (ja) タンパク質分画デバイスおよびタンパク質の分画・濃縮方法
JPH03169897A (ja) 化学修飾エクオリンとその調製法
Shallan et al. On-chip nanofilters for biological sample pre-treatment for electrophoretic analysis of small molecules in whole blood

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20201103

CF01 Termination of patent right due to non-payment of annual fee