CN104783298A - Preparation and application of biological sterilizing composition and nanoemulsion thereof - Google Patents
Preparation and application of biological sterilizing composition and nanoemulsion thereof Download PDFInfo
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- 239000000203 mixture Substances 0.000 title claims abstract description 59
- 239000007908 nanoemulsion Substances 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 66
- 238000000034 method Methods 0.000 claims abstract description 30
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- 239000002253 acid Substances 0.000 claims abstract description 18
- 230000007062 hydrolysis Effects 0.000 claims abstract description 13
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 13
- 239000003910 polypeptide antibiotic agent Substances 0.000 claims abstract description 13
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 240000005528 Arctium lappa Species 0.000 claims abstract description 7
- 235000003130 Arctium lappa Nutrition 0.000 claims abstract description 7
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- 244000223014 Syzygium aromaticum Species 0.000 claims abstract description 7
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- 244000234281 Tamarix gallica Species 0.000 claims abstract description 7
- 235000014265 Tamarix gallica Nutrition 0.000 claims abstract description 7
- 244000061520 Angelica archangelica Species 0.000 claims abstract description 5
- 235000002767 Daucus carota Nutrition 0.000 claims abstract description 5
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- 229920001308 poly(aminoacid) Polymers 0.000 claims description 21
- 230000000844 anti-bacterial effect Effects 0.000 claims description 14
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 13
- GHVNFZFCNZKVNT-UHFFFAOYSA-N decanoic acid Chemical compound CCCCCCCCCC(O)=O GHVNFZFCNZKVNT-UHFFFAOYSA-N 0.000 claims description 12
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 10
- 239000005632 Capric acid (CAS 334-48-5) Substances 0.000 claims description 6
- 244000037364 Cinnamomum aromaticum Species 0.000 claims description 6
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 claims description 6
- 240000007232 Illicium verum Species 0.000 claims description 6
- 235000008227 Illicium verum Nutrition 0.000 claims description 6
- 241000612118 Samolus valerandi Species 0.000 claims description 6
- 239000003899 bactericide agent Substances 0.000 claims description 6
- POULHZVOKOAJMA-UHFFFAOYSA-M dodecanoate Chemical compound CCCCCCCCCCCC([O-])=O POULHZVOKOAJMA-UHFFFAOYSA-M 0.000 claims description 6
- 229940070765 laurate Drugs 0.000 claims description 6
- KWIUHFFTVRNATP-UHFFFAOYSA-N Betaine Natural products C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 5
- PIICEJLVQHRZGT-UHFFFAOYSA-N Ethylenediamine Chemical compound NCCN PIICEJLVQHRZGT-UHFFFAOYSA-N 0.000 claims description 5
- KWIUHFFTVRNATP-UHFFFAOYSA-O N,N,N-trimethylglycinium Chemical compound C[N+](C)(C)CC(O)=O KWIUHFFTVRNATP-UHFFFAOYSA-O 0.000 claims description 5
- -1 N-hydroxysuccinimide imines ester Chemical class 0.000 claims description 5
- 229910019142 PO4 Inorganic materials 0.000 claims description 5
- 229920001213 Polysorbate 20 Polymers 0.000 claims description 5
- 125000001931 aliphatic group Chemical group 0.000 claims description 5
- 229960003237 betaine Drugs 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 235000013336 milk Nutrition 0.000 claims description 5
- 239000008267 milk Substances 0.000 claims description 5
- 210000004080 milk Anatomy 0.000 claims description 5
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 5
- 239000010452 phosphate Substances 0.000 claims description 5
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims description 5
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 241001355283 Cayratia Species 0.000 claims description 4
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 claims description 4
- 239000009636 Huang Qi Substances 0.000 claims description 4
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- 238000005886 esterification reaction Methods 0.000 claims description 4
- 235000015112 vegetable and seed oil Nutrition 0.000 claims description 4
- 239000008158 vegetable oil Substances 0.000 claims description 4
- NQTADLQHYWFPDB-UHFFFAOYSA-N N-Hydroxysuccinimide Chemical compound ON1C(=O)CCC1=O NQTADLQHYWFPDB-UHFFFAOYSA-N 0.000 claims description 3
- 235000019484 Rapeseed oil Nutrition 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000004006 olive oil Substances 0.000 claims description 3
- 235000008390 olive oil Nutrition 0.000 claims description 3
- 239000003549 soybean oil Substances 0.000 claims description 3
- 235000012424 soybean oil Nutrition 0.000 claims description 3
- 235000019483 Peanut oil Nutrition 0.000 claims description 2
- 235000005687 corn oil Nutrition 0.000 claims description 2
- 239000002285 corn oil Substances 0.000 claims description 2
- 239000000312 peanut oil Substances 0.000 claims description 2
- 239000000419 plant extract Substances 0.000 claims description 2
- 239000008159 sesame oil Substances 0.000 claims description 2
- 235000011803 sesame oil Nutrition 0.000 claims description 2
- 102000011782 Keratins Human genes 0.000 abstract description 11
- 108010076876 Keratins Proteins 0.000 abstract description 11
- 239000000047 product Substances 0.000 abstract description 10
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 235000008078 Arctium minus Nutrition 0.000 abstract description 3
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract description 2
- 239000003531 protein hydrolysate Substances 0.000 abstract description 2
- 241000045403 Astragalus propinquus Species 0.000 abstract 1
- 240000002779 Cayratia japonica Species 0.000 abstract 1
- 241000723347 Cinnamomum Species 0.000 abstract 1
- 240000004760 Pimpinella anisum Species 0.000 abstract 1
- 235000012550 Pimpinella anisum Nutrition 0.000 abstract 1
- 235000006533 astragalus Nutrition 0.000 abstract 1
- 230000002195 synergetic effect Effects 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 8
- 239000002994 raw material Substances 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 6
- 238000001291 vacuum drying Methods 0.000 description 5
- 241000231286 Neottia Species 0.000 description 4
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- 239000003795 chemical substances by application Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 235000021511 Cinnamomum cassia Nutrition 0.000 description 2
- 235000002782 Foeniculum vulgare var dulce Nutrition 0.000 description 2
- 240000002045 Guettarda speciosa Species 0.000 description 2
- 229940107666 astragalus root Drugs 0.000 description 2
- 229940008396 carrot extract Drugs 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 229940072117 fennel extract Drugs 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 206010020718 hyperplasia Diseases 0.000 description 2
- 210000001616 monocyte Anatomy 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 229920001184 polypeptide Polymers 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241001225321 Aspergillus fumigatus Species 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000222122 Candida albicans Species 0.000 description 1
- 201000007336 Cryptococcosis Diseases 0.000 description 1
- 241000221204 Cryptococcus neoformans Species 0.000 description 1
- 241001416177 Vicugna pacos Species 0.000 description 1
- 229940091771 aspergillus fumigatus Drugs 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 239000003519 biomedical and dental material Substances 0.000 description 1
- 229940095731 candida albicans Drugs 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 210000000085 cashmere Anatomy 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 210000003746 feather Anatomy 0.000 description 1
- 125000002485 formyl group Chemical class [H]C(*)=O 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
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- 238000002156 mixing Methods 0.000 description 1
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- HOGDNTQCSIKEEV-UHFFFAOYSA-N n'-hydroxybutanediamide Chemical compound NC(=O)CCC(=O)NO HOGDNTQCSIKEEV-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
- A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
- A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
- A23L3/3463—Organic compounds; Microorganisms; Enzymes
- A23L3/3526—Organic compounds containing nitrogen
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/04—Animal proteins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
- A23J3/00—Working-up of proteins for foodstuffs
- A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses preparation and application of a biological sterilizing composition and a nanoemulsion thereof. The biological sterilizing composition comprises tetrapolyamino acid antibacterial peptide mixed ester, and further comprises one or more of extracts of cayratia japonica, tamarix juniperina, star aniseed, fennel, cinnamomum bark, clove, carrot, angelica root, tea tree, burdock and astragalus membranaceus. The biological sterilizing composition has the advantages of high sterilizing bioactivity and good protein hydrolysate compatibility; the biological sterilizing composition is applied to a keratin hydrolysis production process, and through a synergistic effect with a cold atmospheric pressure plasma physical sterilization method, a perfect sterilizing effect on protein liquid is achieved, and the fact that the sanitation quality of a protein product reliably reaches the standard of ISO11290 is ensured.
Description
Technical field
The invention belongs to sterilization technology field, especially relate to a kind of be particularly suitable for keratoprotein preparation in do the biological sterilization composition of bactericidal agent and the preparation and application of nano-emulsion thereof.
Background technology
The mankind have had very long history to the processing and utilization of natural keratin, and wherein plastic products are the earliest that raw material is made with keratin.Biodegradable plastics and biomedical material more and more come into one's own, one of focus wherein studied is exactly from hair, fowl feather etc., extract keratin be used as feed or health nutrient, therefore, for ensureing prepared keratinous-protein product safety and sanitation, in its preparation process, sterilizing is absolutely necessary a processing step.Because keratin belongs to determination system of thermal unstable material, thus conventional Pasteur's high-temp sterilizing method should not be adopted, chemical disinfection sterilization can not be adopted, sterilizing methods in current keratin production is mainly microwave drying sterilization or cold atmospheric pressure plasma sterilizing, but alone above-mentioned physical disinfection sterilization, products obtained therefrom quality does not reach the health matter standard of ISO11290, particularly do not kill the Listera spp of the serious monocyte hyperplasia of harmfulness, and this bacterium all takes zero-tolerance policy in the food inspection management of the developed countries such as the U.S..
Summary of the invention
An object of the present invention is to provide a kind of especially high to Listera spp body killing rate biological sterilization composition; Two of object of the present invention is to provide a kind of method this biological sterilization composition being prepared into nano-emulsion; Three of object of the present invention is applied to by above-mentioned biological sterilization composition in keratoprotein hydrolysis production technology, and with the synergy of cold atmospheric pressure plasma physical sterilization method synergism, make protein liquid reach perfect sterilization effect, ensure that protein product hygienic quality reliably reaches the standard of ISO11290.
The technical solution realizing one of above-mentioned purpose is:
A kind of biological sterilization composition, comprises four polyaminoacid antibacterial peptide mixed esters.
As preferably, also comprise one or more of extract of Japanese Cayratia Herb or tamarix juniperina Bunge, illicium verum, fennel seeds, cassia, clove tree, carrot, angelica root, tea tree, great burdock achene, the Radix Astragali.
As preferably, described sterilization composition comprises four polyaminoacid antibacterial peptide mixed ester 6-9 wt% and following plant extracts: Japanese Cayratia Herb, 33-36wt%, tamarix juniperina Bunge, 8-10wt%, illicium verum, 3-4 wt%, fennel seeds, 0.6-1 wt%, cassia, 7-9 wt%, clove tree, 0.6-1 wt%, carrot, 3-4 wt%, angelica root, 5-8 wt%, tea tree, 1-2 wt%, great burdock achene, 15-20 wt%, the Radix Astragali, 4-6 wt%.
As preferably, described four polyaminoacid antibacterial peptide mixed esters are the mixed ester of four polyaminoacid antibacterial peptides and mixed acid esterification.
Further preferably, described mixed acid is capric acid 60-90wt% and laurate 10-40wt%.
As preferably, the preparation method of the mixed ester of described four polyaminoacid antibacterial peptides and mixed acid esterification is:
Four polyaminoacid antibacterial peptides and concentration are the capric acid 60-90wt% of 0.5-1.5mol/l, laurate 10-40wt% mixed acid mixes mutually, add the N-hydroxysuccinimide with mixed acid same concentrations again, under dicyclohexylcarbodiimide existence condition, room temperature condensation, obtains the N-hydroxysuccinimide imines ester of aliphatic acid.
The technical solution realizing above-mentioned purpose two is:
The preparation method of the nano-emulsion of described biological sterilization composition, comprises the following steps: material rate is:
1) in 50ml deionized water, add phosphate and allot the cushioning liquid that pH value is 7-7.5, add coamidopropyl betaine 200g, add in homogenizer and process 2-8min at 40-60 DEG C;
2) complete for sterilization milk 400-600ml, ethanol 400-600ml, Tween-20 20-30g are added in homogenizer, at 40-60 DEG C, process 2-8min;
3) the biological sterilization composition 19-22g described in any one of claim 1-6 is added in 1200-1300g vegetable oil, at being placed in homogenizer 40-55 DEG C, stir 10-20min, then by step 2) gained mixture is added in step 1) gained mixture, add in homogenizer, at 40-60 DEG C, process 5-15min;
4) in step 3) gained mixed liquor, add ethylenediamine two amber ester, in nanometer emulsified device, process 15-30min at 30-50 DEG C, break into nano-emulsion.
As preferably, described vegetable oil is soybean oil, rapeseed oil, peanut oil, sesame oil, corn oil or olive oil.
Described biological sterilization composition in keratoprotein hydrolysis as the application of bactericidal agent;
As preferably, the addition of described biological sterilization composition is 1.2-1.4kg/100kg keratoprotein raw material.
The technical solution realizing above-mentioned purpose three is:
In 100Kg hydrolysis of keratin liquid, add 1.2-1.4kg biological sterilization agent composition nano-emulsion, after adopting cold atmospheric pressure plasma physical sterilization method to work in coordination with sterilizing, carry out vacuum drying simultaneously, obtained albumen powder product.
Beneficial effect of the present invention is:
1, four polyaminoacid antibacterial peptides, the research on maximum utilized quantity of its antibacterial Cmin is 12.5mM, electrostatic charge+3, molecular weight 572, and its active function site sequence is: rely amino-leucine-Trp-Ly-NH
2when poly-peptide is larger than four for the degree of polymerization, toxicity increases, sterilization effect is poor on the contrary, in order to improve sterile biological activity and the compatibility with protein hydrolysate thereof, the present invention selects the mixed ester of four polyaminoacid antibacterial peptides, namely four polyaminoacid antibacterial peptides and capric acid 60-90wt%, the mixing of laurate 10-40 wt% is adopted, add N-hydroxy-succinamide again, and under dicyclohexylcarbodiimide exists, after room temperature condensation, obtain the N-hydroxy-succinamide ester of aliphatic acid, the good stability of the N-hydroxy-succinamide ester of aliphatic acid, can not react with fat carboxyl and generate aldehyde; The fusing point of four polyaminoacid antibacterial peptide antibacterial peptide mixed esters, at about 70 DEG C, has higher biologically active and the compatibility of protein.
2, biological sterilization active compound is water-soluble very poor, and in order to increase antibacterial activity, its bioactive composition is made the nanoemulsions that particle diameter is 800-950nm by the present invention, can improve the solubility of composite antibacterial activity composition, thus increase its antibacterial activity.
3, composite sterilization composition provided by the invention in keratoprotein hydrolysis as the application of bactericidal agent, be highly resistant to Listera spp and Candida albicans (ATCC10231), Cryptococcus neoformans (ATCC MYA-422), aspergillus fumigatus (ATCC26430), meet the Listera spp of developed country to monocyte hyperplasia and take zero-tolerance policy on food.By experimental results demonstrate that being hydrolyzed (containing 600l treatment fluid) in keratin raw material at 100Kg adds 1.2-1.4 grams per liter complex biological bactericidal agents, nutrition (polypeptide) content is high, color and luster good, compound ISO11290 standard, i.e. unharmful substance.
Detailed description of the invention
With specific embodiment, the present invention will be further described below, therefore do not limit the present invention among described scope of embodiments.
Embodiment 1, prepares biological sterilization composition
First prepare four polyaminoacid antibacterial peptide mixed esters: be that capric acid 60-90wt%, the laurate 10-40wt% mixed acid of 0.5-1.5mol/l mixes mutually by four polyaminoacid antibacterial peptides and concentration, add the N-hydroxysuccinimide with mixed acid same concentrations again, under dicyclohexylcarbodiimide existence condition, room temperature condensation, obtains the N-hydroxysuccinimide imines ester of aliphatic acid.
Biological sterilization composition A: Herba Cayatiae Japonicae extract 33wt%, tamarix juniperina Bunge extract 10wt%, illicium verum extract 3 wt%, sweet fennel extract 1 wt%, extract of cinnamomum cassia bark 7wt%, clove tree extract 1 wt%, carrot extract 3wt%, Angelica roots 8 wt%, tea tree extract 1wt%, burdock extract 20wt%, Astragalus Root P.E 4wt% and four polyaminoacid antibacterial peptide mixed ester 9 wt%.
Biological sterilization composition B:
Herba Cayatiae Japonicae extract 36wt%, tamarix juniperina Bunge extract 8wt%, illicium verum extract 4 wt%, sweet fennel extract 0.6 wt%, extract of cinnamomum cassia bark 9 wt%, clove tree extract 0.6wt%, carrot extract 4 wt%, Angelica roots 5wt%, tea tree extract 2 wt%, burdock extract 15wt%, Astragalus Root P.E 6wt% and four polyaminoacid antibacterial peptide mixed ester 6wt%.
Biological sterilization composition C:
Herba Cayatiae Japonicae extract 91wt%, four polyaminoacid antibacterial peptide mixed esters, 9 wt%.
Embodiment 2, prepares biological sterilization composition nano-emulsion
1) in 50ml deionized water, add phosphate and allot the cushioning liquid that pH value is 7, add coamidopropyl betaine 200g, add in homogenizer and process 2min at 60 DEG C;
2) complete for sterilization milk 400ml, ethanol 400ml, Tween-20 20g are added in homogenizer, at 60 DEG C, process 2min;
3) biological sterilization composition A being added in soybean oil, is placed in homogenizer 55 DEG C, stir 10min, then by step 2) gained mixture is added in step 1) gained mixture, adds in homogenizer, process 5min at 60 DEG C.
4) in step 3) gained mixed liquor, add 150mg ethylenediamine two amber ester, in nanometer emulsified device, at 50 DEG C, process 15min, break into the nano-emulsion of 800nm.
embodiment 3,prepare biological sterilization composition nano-emulsion
1) in 50ml deionized water, add phosphate and allot the cushioning liquid that pH value is 7.5, add coamidopropyl betaine 200g, add in homogenizer and process 8min at 40 DEG C;
2) complete for sterilization milk 400ml, ethanol 600ml, Tween-20 30g are added in homogenizer, at 40 DEG C, process 8min;
3) biological sterilization composition B being added in olive oil, at being placed in homogenizer 40 DEG C, stirs 20min, then by step 2) gained mixture is added in step 1) gained mixture, adds in homogenizer, process 15min at 40 DEG C.
4) in step 3) gained mixed liquor, add 100g ethylenediamine two amber ester, in nanometer emulsified device, at 30 DEG C, process 30min, break into the nano-emulsion of 900nm.
embodiment 4,prepare biological sterilization composition nano-emulsion
1) in 50ml deionized water, add phosphate and allot the cushioning liquid that pH value is 7, add coamidopropyl betaine 200g, add in homogenizer and process 5min at 50 DEG C;
2) complete for sterilization milk 600ml, ethanol 400ml, Tween-20 25g are added in homogenizer, at 50 DEG C, process 5min;
3) biological sterilization composition C being added in rapeseed oil, at being placed in homogenizer 45 DEG C, stirs 15min, then by step 2) gained mixture is added in step 1) gained mixture, adds in homogenizer, process 10min at 50 DEG C.
4) in step 3) gained mixed liquor, add 200mg ethylenediamine two amber ester, in nanometer emulsified device, at 40 DEG C, process 25min, break into the nano-emulsion of 950nm.
embodiment 5,application to biological sterilization composition in keratoprotein hydrolysis production technology
In 100Kg cashmere fiber hydrolysis of keratin raw material, (containing 600l treatment fluid) adds the biological sterilization agent composition nano-emulsion of 1.2kg embodiment 2 gained, at 40 DEG C, after adopting cold atmospheric pressure plasma physical sterilization method to work in coordination with sterilizing, carry out vacuum drying, obtained albumen powder product simultaneously.
Embodiment 6, the application to biological sterilization composition in keratoprotein hydrolysis production technology
In 100Kg mohair yarn fiber hydrolysis of keratin raw material, (containing 600l treatment fluid) adds the biological sterilization agent composition nano-emulsion of 1.3kg embodiment 3 gained, at 40 DEG C, after adopting cold atmospheric pressure plasma physical sterilization method to work in coordination with sterilizing, carry out vacuum drying, obtained albumen powder product simultaneously.
Embodiment 7, the application to biological sterilization composition in keratoprotein hydrolysis production technology
In 100Kg light color alpaca hydrolysis of keratin raw material, (containing 600l treatment fluid) adds the biological sterilization complex composition nano-emulsion of 1.4kg embodiment 4 gained, at 40 DEG C, after adopting cold atmospheric pressure plasma physical sterilization method to work in coordination with sterilizing simultaneously, carry out vacuum drying, obtained albumen powder product.
Comparative examples
Enzymolysis raw material is identical with embodiment 7, but in enzymolysis liquid, do not add sterilizing activity thing, after only adopting the sterilizing of cold atmospheric pressure plasma physical sterilization method, carries out vacuum drying, obtained albumen powder product.
Produced by albumen powder obtained by embodiment 5,6,7 and comparative examples and carry out hygienic quality detection, result is as following table:
As can be seen from the table, do not add biological sterilization active matter in comparative example, sterilization effect does not reach the standard of ISO11290.
Embodiment 5-7 biological sterilization active matter carries out sterilizing, and the testing result of the albumen powder after sterilizing all can reach ISO11290 standard-required, and the accounting of polypeptide in albumen powder is higher, and nutrition is abundant.
Claims (10)
1. a biological sterilization composition, is characterized in that comprising four polyaminoacid antibacterial peptide mixed esters.
2. biological sterilization composition according to claim 1, characterized by further comprising: one or more of the extract of Japanese Cayratia Herb or tamarix juniperina Bunge, illicium verum, fennel seeds, cassia, clove tree, carrot, angelica root, tea tree, great burdock achene, the Radix Astragali.
3. biological sterilization composition according to claim 2, is characterized in that, described sterilization composition comprises four polyaminoacid antibacterial peptide mixed ester 6-9 wt% and following plant extracts: Japanese Cayratia Herb, 33-36wt%, tamarix juniperina Bunge, 8-10wt%, illicium verum, 3-4 wt%, fennel seeds, 0.6-1 wt%, cassia, 7-9 wt%, clove tree, 0.6-1 wt%, carrot, 3-4 wt%, angelica root, 5-8 wt%, tea tree, 1-2 wt%, great burdock achene, 15-20wt%, the Radix Astragali, 4-6 wt%.
4. biological sterilization composition according to claim 1, is characterized in that, described four polyaminoacid antibacterial peptide mixed esters are the mixed ester of four polyaminoacid antibacterial peptides and mixed acid esterification.
5. biological sterilization composition according to claim 4, is characterized in that, described mixed acid is capric acid 60-90wt% and laurate 10-40wt%.
6. the biological sterilization composition according to claim 4 or 5, is characterized in that, the preparation method of the mixed ester of described four polyaminoacid antibacterial peptides and mixed acid esterification is:
Four polyaminoacid antibacterial peptides and concentration are the capric acid 60-90wt% of 0.5-1.5mol/l, laurate 10-40wt% mixed acid mixes mutually, add the N-hydroxysuccinimide with mixed acid same concentrations again, under dicyclohexylcarbodiimide existence condition, room temperature condensation, obtains the N-hydroxysuccinimide imines ester of aliphatic acid.
7. a preparation method for the nano-emulsion of biological sterilization composition as claimed in claim 1, is characterized in that, comprise the following steps: material rate is:
In 50ml deionized water, add phosphate allot the cushioning liquid that pH value is 7-7.5, add coamidopropyl betaine 200g, add in homogenizer and process 2-8min at 40-60 DEG C;
Complete for sterilization milk 400-600ml, ethanol 400-600ml, Tween-20 20-30g are added in homogenizer, at 40-60 DEG C, processes 2-8min;
Biological sterilization composition described in any one of claim 1-6 is added in vegetable oil, at being placed in homogenizer 40-55 DEG C, stirring 10-20min, then by step 2) gained mixture is added in step 1) gained mixture, add in homogenizer, at 40-60 DEG C, process 5-15min;
In step 3) gained mixed liquor, add ethylenediamine two amber ester, in nanometer emulsified device, process 15-30min at 30-50 DEG C, break into nano-emulsion.
8. the preparation method of the nano-emulsion of biological sterilization composition according to claim 7, it is characterized in that, described vegetable oil is soybean oil, rapeseed oil, peanut oil, sesame oil, corn oil or olive oil.
9. a biological sterilization composition according to claim 1 in keratoprotein hydrolysis as the application of bactericidal agent.
10. biological sterilization composition according to claim 9 is as the application of bactericidal agent in hydrolysis keratoprotein liquid, it is characterized in that, the addition of described biological sterilization composition is that 1.2-1.4kg/100kg is hydrolyzed keratoprotein liquid.
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