CN105145657A - Environment-friendly mosquito repellent preparing method - Google Patents

Abstract

The invention relates to an environment-friendly mosquito repellent preparing method. Mosquitoes can bring inconvenience to people in life. According to the method, traditional Chinese medicine is adopted, and toxic components are prepared in a biological way and combined to form a traditional Chinese medicine mosquito repellent. The effect is good, environment friendliness is realized, low price and high quality are realized, no pollution is caused in production, large-scale production and industrialized production can be achieved, raw materials are obtained from the nature, and resources are abundant.

Description

A kind of environmental protection culicifuge preparation method

Technical field

The art relates to a kind of environmental protection culicifuge preparation method.Due to a large amount of uses of chemicals, severe contamination is caused to ecotope, cause that animal is poisoning, genetic mutation, cause people's cancerous swelling incidence of disease sharply to raise.Remaining of grain agricultural chemicals, remaining of pesticide in vegetable, remaining of feed agricultural chemicals, directly threatens human body health.And current mosquito causes the impact that people live, the consumption of chemicals is increasing, pollutes more and more serious, and be therefore badly in need of developing insecticide without environmental pollution, green Chinese insecticide is more and more subject to the close amidine of people.

Background technology

The life of mosquito to people causes very big inconvenience, and fast spreading disease.The main harm of mosquito spreads disease.According to the study, the disease of mosquitoes spread reaches more than kind more than 80.On earth, then do not have which kind of animal to have larger harm than mosquito to the mankind.This disease of malaria is by malaria mosquito-borne.Malaria is also known as miasma.According to the investigation of nineteen thirty-five Department of Health, in the region that miasma is prevailing, have and plasmodially account for 50% in resident's blood, that suffers from pernicious malaria accounts for 72%.Within 1936, Jiang Sugao county is because of about dead 20,000 people of trouble malaria, and once because of yellow fever and the former workman countless in the dust of malaria during the cutting Panama Canal in 1876, so that 1889 must not stop work then.The problem solving mosquito by entomologist has just been continued canal engineering.The nineteen thirty report of Far East tropical disease medical association is pointed out: Thailand dies from tiger's jaw about 50 people every year, and dies from malaria person and reach 50,000 people.Mosquito be how cause of disease is imported into human body suck the blood suffering from malaria patient, also in plasmodium (pathogeny of malaria) inspiration body wherein when malarial mosquito.When they sting people again, plasmodium has been injected in the body of the person of being snapped again from the mouth of mosquito.After ten days, plasmodium starts to occur at the Ink vessel transfusing close to skin.They breed in the erythrocyte of patient, split into a large amount of little protozoons, and these little protozoons are destroyed erythrocyte and discharge a kind of toxin.Each little plasmodium invades again other erythrocyte and continues breeding, plasmodium and toxin in patient body is got more and more, causes patient to feel cold and have a fever.First the patient obtaining malaria feels cold, and whole body is trembled and not stopped, but thermometer test body temperature is high.Approximately pass through one hour, patient just feels to have a fever, and at this moment body temperature continues to rise, and start perspiration, temperature decline after three, four hours, feel relaxed after several hours patients, disease has seemed to pass by, and at this moment little protozoon has invaded new erythrocyte in fact, starts again to breed.When plasmodium is again destroyed erythrocyte and goes out, patient falls ill and forms second leg.Unless obtain suitable treatment this outbreak will continue regularly and painful can't bear.Malaria is sizable to the loss that the mankind cause, and patient body is weak, inefficiency, also can loss of life time serious.At present can treat and prevent this sick with medicine, but best bet be eliminate infect this disease mosquito--malaria loses.Japanese Type-B encephalitis (this is a kind of acute infectious disease caused by filterable virus), also pass band by mosquito, this disease is Japanese B encephalitis again, commonly all it is called brain inflammation.Patient has fever, headache, vomiting, the phenomenons such as exhausting, lethargic sleep, stupor.Treatment do not have special effect medicine, so case fatality rate is quite high.Mosquito is just provided with infectivity through a suitable period, then bites when not having immune people the person of being snapped can be made to fall ill after stinging and having inhaled and close and have the blood of the human or animal of this virus.The mosquito infecting this disease cries culex and yellow-fever mosquito.The Occurrence & epidemic of anti-disease of effecting a permanent cure, except vaccination, control infect, must carry out kill mosquito energetically, eliminate disseminator.In addition, culex and anopheles also propagate filariosis (elephant hide swells).Traditional Chinese medicine extraction insecticide is utilized to replace chemical pesticide progressively in formation.

Summary of the invention

The present invention relates to a kind of environmental protection culicifuge preparation method: application Pesticide with Chinese medicinal material is nuisanceless, and environmental protection, respond well, insect pest, without variation, has no drug resistance, and medicine source derives from the Nature and goes back to nature.This preparation method utilizes biotechnology fermentation technique, extract following Chinese medicine toxic component and be combined into Pesticide with Chinese medicinal material: thunder godvine (CommonThreewingnutRoot), root is containing wilfordine (wilfordine), wilforine (wilforine), Wilforgine (wilforgine), Wilfortrine and wilfortrine (wil-skin acid amides (celacinnine), celastrus orbiculatus furancarboxylic acid amine (celafurine), celastrus orbiculatus benzyl acid amides (celagbenzine), Triptolide (wilforlide) A, B, triptonoterpenol (triptonoterpenol), 16-hydroxytriptolide (16-hydroxytripto-lide), triptolide and triptolide (triptolide), table Triptolide triol (epitriptriolide), thunder kaurene lactone (tripterifordine), mapping-Lei kaurene lactone (antriptolactone), wilfordic acid (tripterygicacid), straight wedge oxalic acid (orthosphenicacid), cupreol (β-sitosterol) and Daucosterol (daucosterol).The property of medicine: toil, very toxic.Effect: dispel the wind, removing toxic substances, desinsection.Fructus gleditsiae (FructusGleditsiaeAbnormalis), fructus gleditsiae fruit is containing saponin.Hydrolysis generates sapogenin, and its glucoside unit is echinocystic acid.The property of medicine: warm in nature, taste is pungent, slightly poisonous.Effect: dispel the wind and have one's ideas straightened out, searches wind desinsection.Vomiting nut (NuxVomica, DongButton), containing indoles alkaloid, total alkali 3% ~ 5%, wherein brucine (strychnine, strychnine) be main active, secondly be strychnia (brucine), still containing lot of trace alkaloid, as α-and β-colubrine (α-, β-colubrine), neobrucine (isostrychnine), pseudostrychnine (pseudostrychnine), pseudobrucine (pseudobrucine), vomicine (vomicine), novacine (novacine), icajine (icajine) etc.The property of medicine: cold in nature, bitter, very toxic.Effect: loose blood-head, swelling and pain relieving, desinsection.Crotons (crotonfruit), plant benevolence fatty oily Crothonoil and are about 40-60%, containing croton resin in oil, be crotonyl alcohol, ester that formic acid, butyric acid and crotonic acid are combined into, have strong catharsis effect.In addition, containing protein about 18%, comprising a kind of toxicity globulin, crotin is claimed.The another alkaloid containing crotonoside 1-3.8%, arginine, lysine, lipolytic enzyme and a kind of similar recinine.Containing epicarcinogen in croton oil.For colourless resin thing, after hydrolysis, produce epicarcinogen A3 and carcinogenic substance B2.The property of medicine: taste is pungent, very toxic.Effect: drastic purgation stagnates, and detumescence of relieving oedema or abdominal distension through diuresis or purgation, eliminates the phlegm, desinsection.Large maple (SemenHydnocarpi), originates as the mature seed of large maple scarabaeidae plant chaulmoogra.Seed, containing the fat oil of volume, claims chaulmoogra oil, accounts for 50%, and its main component is the sour and secondary large maple acid of large maple and secondary hyrganol, dispel hydrogen hyrganol and glyceride thereof.The property of medicine: pungent, heat, poisonous.Cure mainly (effect): dispel pathogenic wind and remove dampness, detoxicating and destroying parasites.Control leprosy, mange, syphilis.The dried venom of toads (VENENUMBUFONIS), cardiac stimulant steroidal compounds is similar cardiac glycoside and virose bufotalin compounds, there will be a known more than 10 and plants, the catabolite in dry process mostly, as cinobufagin about 5%, resibufogenin about 3.4%, Bufalin about 1.8%, hydroxyl China toadpoison base about 1.6%, bufalin (bufotalin) about 1.5%, magnificent toadpoison base (telocinobufagin) about 1.4% far away and marinobufagin (marinobufagin) etc.; Another containing digitoxigenin, hispidagenin etc. be everlasting C3 and suberylarginine, heptanedioyl arginine, succinyl arginine, suberic acid, sulfuric acid etc. of above-mentioned bufotalin is combined into ester class, be referred to as toadpoison class, be present in the toad secretion before processing more. the contamination of bufotalin and toadpoison in the dried venom of toads, can be different and variant with method because of former animal, the place of production, time of gathering and processing.The property of medicine: sweet, acrid, warm, poisonous.Effect: removing toxicity for detumescence, understand things pain-stopping, desinsection.In the Nature, insect has hundreds of kind, some insect has function of detoxification, independent 1 ~ 2 kind of Chinese medicine insecticidal effect is bad, must arrange two or more toxic herbs and carry out desinsection and just have comprehensive killing effect, thus above six kinds of toxic herb extracts are merged into prescription insecticide and carry out desinsection.This Pesticide with Chinese medicinal material innovative point, insecticidal effect is good, and nuisanceless, insect does not make a variation, and without pesticide resistance, produces environment friendly and pollution-free, and raw material sources are in the Nature, and supplied materials is abundant, low price, can scale, factorial praluction.

Embodiment

Below in conjunction with embodiment, the present invention is further elaborated

Embodiment one

Get containing the abundant crotons of grease, each 400 grams of large maple, powder process 40 order, plus wheat bran 100 grams again, add water 75%, mix thoroughly, 1 kg/cm sterilizing 20 minutes, dry in the air to 25 DEG C, inoculate 161 Trichoderma virides and 3.342 aspergillus nigers each 1%, be incubated 25 DEG C, carry out solid koji, cultivate 72 hours, add 3000 ml tap waters, be incubated 45 DEG C, through 72 hours enzymolysis, carry out 45 DEG C of hot reflux lixiviates 2 hours again, again with 2000 milliliters of 45 DEG C of hot water thermal backflow lixiviates 2 hours, twice leaching liquor is merged, deliver to vacuum-concentrcted, evaporating temperature 60 DEG C, concentrated 1/3rd, receive concentrate 2000 milliliters.(mould, the sweet potato aspergillus niger of 161 wood is containing holoenzyme system: the multiple enzymes such as amylase, lipase, protease, pectase.）

Embodiment two

Get fructus gleditsiae, vomiting nut, each 400 grams of thunder godvine, powder process 40 order, the running water of 8 times is added in material, add the 161 Trichoderma viride cellulases of 1%, 1% 3.942, protease, the Rhiz.amylase of 1%, the mould pectase of sweet potato etc. of 1% inserts the abundant stirring and evenly mixing of pot for solvent extraction, steam heats 45 DEG C, enzymolysis is after 72 hours, the agitator started on pot for solvent extraction stirs, to promote the leaching velocity of active ingredient, extraction time 2 hours, with 100 order filter-cloth filterings, filtrate is through vacuum-concentrcted 1/3rd, thickening temperature 60 DEG C, obtain solution 3000 milliliters, for subsequent use.

Embodiment three

Remove the toad after internal organ 400 grams, insert meat grinder strand to muddy flesh, the water then adding 3 times enters colloid mill grinding, fineness is below 0.001 millimeter, insert biochemical reaction tank, through 80 DEG C of sterilizings 20 minutes, dry in the air to 40 DEG C, add 3.924 and 1.398 compound proteases 1%, 3.324 lipase from Aspergillus Nigers 1%, are incubated 45 DEG C, enzymolysis 72 hours, then carry out centrifugation, obtain solution 1800 milliliters.

Embodiment four

Collect embodiment one, two, three solution, amount to 6800 milliliters, mixing is finished product.Blend with the water of 1:50, sprinkling is carried out to insect and kills.

Claims (4)

1. the present invention relates to a kind of environmental protection culicifuge preparation method, crotons are extracted by biotechnology, large maple, vomiting nut, fructus gleditsiae, toad, the toxic component of thunder godvine is combined into insecticide, it is characterized in that, get containing the abundant crotons of grease, large maple, powder process 40 order, plus wheat bran again, add water 75%, mix thoroughly, 1 kg/cm sterilizing 20 minutes, dry in the air to 25 DEG C, inoculate 161 Trichoderma virides and 3.342 aspergillus nigers each 0.3% ~ 1%, be incubated 20 DEG C ~ 25 DEG C, carry out solid koji, cultivate 48-72 hours, add 3000 ml tap waters, be incubated 40 DEG C ~ 45 DEG C, through 48-72 hours enzymolysis, carry out 40 DEG C ~ 45 DEG C hot reflux lixiviates 1-2 hours again, use 2000 milliliters of 40 DEG C ~ 45 DEG C hot water thermal backflow lixiviates 1-2 hours again, twice leaching liquor is merged, deliver to vacuum-concentrcted, concentrated 1/3rd, steam Ba temperature 60 C for subsequent use.

2. the present invention relates to a kind of environmental protection culicifuge preparation method, according to claims 1, to fructus gleditsiae, vomiting nut, producing of thunder godvine, it is characterized in that, get fructus gleditsiae, vomiting nut, thunder godvine, powder process 40 order, the running water of 6-8 times is added in material, add the 161 Trichoderma viride cellulases of 0.3% ~ 1%, 0.3% ~ 1% 3.942, protease, the Rhiz.amylase of 0.3% ~ 1%, the mould pectase of sweet potato etc. of 0.3% ~ 1% inserts the abundant stirring and evenly mixing of pot for solvent extraction, steam heats 40 DEG C ~ 45 DEG C, enzymolysis is after 48-72 hours, the agitator started on pot for solvent extraction stirs, to promote the leaching velocity of active ingredient, extraction time 1-2 hours, with 100 order filter-cloth filterings, filtrate is through vacuum-concentrcted 1/3rd, thickening temperature 60 DEG C, for subsequent use.

3. the present invention relates to a kind of environmental protection culicifuge preparation method, according to claims 1, producing of toad body, it is characterized in that: remove the toad after internal organ, insert meat grinder strand to muddy flesh, the water then adding 2-3 times enters colloid mill grinding, and fineness is below 0.001 millimeter, insert biochemical reaction tank, through 75 DEG C of-80 DEG C of sterilizings 20 minutes, dry in the air to 40 DEG C, add 3.924 and 1.398 compound proteases 03% ~ 1%, 3.324 lipase from Aspergillus Nigers 0.3% ~ 1%, be incubated 45 DEG C, enzymolysis 48-72 hours, then carries out centrifugation, collect solution, for subsequent use.

4. the present invention relates to a kind of environmental protection culicifuge preparation method, according to claims 1,2,3, it is characterized in that: collect embodiment one, two, three solution, mixing, is finished product, blends with the water of 1:50, carry out sprinkling kill insect.