CN103933156A - Antibacterial plant active composition and extraction and preparation method thereof - Google Patents
Antibacterial plant active composition and extraction and preparation method thereof Download PDFInfo
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Abstract
The invention provides an antibacterial plant active composition and an extraction and preparation method thereof. The method comprises the following steps: respectively extracting honeysuckle, isatis root, astragalus, hawthorn, liquorice and the like serving as main medicinal materials once by using water, ethanol and an ethyl acetate solvent; obtaining the extract of each medicinal material after concentrating and mixing, wherein these medicinal materials can be extracted after being mixed, or mixed after being extracted, so as to obtain the plant active composition. In addition, the antibacterial plant active composition can also be assorted with the root of Chinese pulsatilla and sculellaria barbata. The immunity of an animal is improved by using the antibacterial plant active components through the synergistic interaction effect of the composition ratio of the extracted antibacterial plant active composition, and the antibacterial and bacteriostatic effects are achieved while use of antibiotics or drugs is avoided, so as to provide a safe breeding condition which is natural, free of a drug and healthy.
Description
Technical field
The present invention relates to a kind of vegetable active extract, particularly relate to a kind ofly in breeding process, can increase the biocidal property reactive composition of plants of the antibacterial or antibacterial effect of cultivated animals and extract preparation method.
Background technology
Aquaculture covering scope is very wide, and it is not only supplied raw materials for industries such as food, pharmacy, oils and fatss, also for people's life provides the abundant foods such as meat, breast, egg, fowl.But in the process of animal cultivation, for avoiding its unlikely infection source of disease bacterium, cultivation dealer generally gets used to using in feeding process antibiotic, or even some chemicals, by being added into feeding cultivated animals in feedstuff, improves animal resistance with this; Or after sick, directly take Drug therapy zoogenetic infection source of disease bacterium.
In cultivated animals, common source of disease bacterium has the source of disease bacterium such as haemophilus parasuis, Salmonella, streptococcus, baby swine paratyphoid bacterium, clostridieum welchii, escherichia coli, staphylococcus aureus, pleuropneumonia radiation bacillus, infect after these source of disease bacterium when animal is unfortunate, cultivation dealer can directly take administering mode, and for example chloromycetin is treated these animals.But these cultivated animals, no matter be to have eaten antibiotic or medicine, are just easy to develop immunity to drugs, through food chain, we mankind eat into these cultivated animals or its product, can develop immunity to drugs at human body equally, and then cause having the generation of superbacteria; Food chain relation in the middle of this, produces like this.And long-term edible medicine residual quantity exceedes the animal products of legal permissible criterion, very large to human injury, the superbacteria that produced when the time comes by without any antibiotic or medicine can resist, this will be the large great calamity of one in mankind's public health.
For good cultivating condition, and meet environmental protection, preferably do not use any antibiotic or medicine.But reverting to aquaculture but may affect because not using antibacterial or medicine cultivation or its product of poultry, causes its price constantly to raise up.In view of this, the present invention then proposes a kind of biocidal property reactive composition of plants and extracts preparation method, the medicine being used while cultivation to replace, and then solve the awkward situation that current aquaculture faces.
Summary of the invention
Main purpose of the present invention is to provide a kind of biocidal property reactive composition of plants, antibiotic and the medicine of required use when it extracts composition replacement cultivation with vegetable active that can be antibacterial, and the effect of the powerful Synergistic having by the portfolio ratio of reactive composition of plants, improve the immunity of animal, effectively reach effect of antibacterial bacteriostatic, and then the natural livestock product without medicine healthy aquaculture is provided.
Extraction is extracted as and achieves the above object, and the present invention proposes a kind of biocidal property reactive composition of plants, and this reactive composition of plants comprises the extract of following medical material: Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae;
By following two kinds of methods, any one prepares described reactive composition of plants:
First method: by Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae respectively first taking water, ethanol and ethyl acetate after solvent extracts, concentrate drying, obtain respectively Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract, the part by weight that is 1～1.5:1～1.5:1～1.5:1～1.5:1～1.5 by Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract according to weight ratio again mixes, and obtains described reactive composition of plants; Or
Second method: the part by weight that is 1～1.5:1～1.5:1～1.5:1～1.5:1～1.5 according to weight ratio by Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae mix again by mixed medical material respectively taking water, ethanol and ethyl acetate after solvent extracts, concentrate drying, obtains the reactive composition of plants that contains Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract.
In addition, the present invention also proposes a kind of extraction preparation method of biocidal property reactive composition of plants, and by following two kinds of methods, any one prepares:
First method:
Provide respectively following medical material: comprise Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae;
By each medical material respectively after the water soaking of 3～10 times with its weight, first, after reflux for the first time, sequentially, after supercooling, filtering, to obtain respectively the first filtrate and the first residue, this first filtrate forms the first concentrated solution after concentrating under reduced pressure;
By this first residue taking 3～10 times of its weight and concentration after 50～95% soak with ethanol, first after reflux for the second time, sequentially, after supercooling, filtering, to obtain respectively the second filtrate and the second residue, this second filtrate forms the second concentrated solution after concentrating under reduced pressure;
By this second residue taking 3～10 times of its weight and concentration after 50～95% ethyl acetate is soaked, first, after reflux for the third time, sequentially, after supercooling, filtering, to obtain the 3rd filtrate, the 3rd filtrate forms the 3rd concentrated solution after concentrating under reduced pressure; And
After being mixed, this first concentrated solution, this second concentrated solution and the 3rd concentrated solution be dried, to obtain the extract of each medical material, make respectively Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract according to above method;
The Flos Lonicerae extract preparing, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract are mixed in proportion and obtain reactive composition of plants;
Second method:
Provide respectively following medical material: comprise Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae, each medical material is mixed in proportion;
After the water soaking of 3～10 times by mixed medical material with its weight, first, after reflux for the first time, sequentially, after supercooling, filtering, to obtain respectively the first filtrate and the first residue, this first filtrate forms the first concentrated solution after concentrating under reduced pressure;
By this first residue taking 3～10 times of its weight and concentration after 50～95% soak with ethanol, first after reflux for the second time, sequentially, after supercooling, filtering, to obtain respectively the second filtrate and the second residue, this second filtrate forms the second concentrated solution after concentrating under reduced pressure;
By this second residue taking 3～10 times of its weight and concentration after 50～95% ethyl acetate is soaked, first, after reflux for the third time, sequentially, after supercooling, filtering, to obtain the 3rd filtrate, the 3rd filtrate forms the 3rd concentrated solution after concentrating under reduced pressure;
After this first concentrated solution, this second concentrated solution and the 3rd concentrated solution are mixed, be dried, obtain reactive composition of plants.Extract
Coordinate appended graphic explanation in detail by specific embodiment below, when the effect that is easier to understand object of the present invention, technology contents, feature and reaches.
Brief description of the drawings
The 1st figure is the medicinal material extract schematic flow sheet that reactive composition of plants of the present invention uses.
Description of reference numerals: 10-the first filtrate; 12-the first residue; 14-the first concentrated solution; 16-the second filtrate; 18-the second residue; 20-the second concentrated solution; 22-the 3rd filtrate; 24-the 3rd concentrated solution; 26-extract.
Detailed description of the invention
Be below embodiment and test data etc. thereof, but content of the present invention is not limited to the scope of these embodiment.
In order to reduce the problem of current aquaculture medication, the present invention replaces when cultivation antibiotic and medicine used with the compositions of active components of plants that can be antibacterial, and the effect of the powerful Synergistic having by the portfolio ratio of reactive composition of plants, effectively improve the immunity of animal, the source of disease of avoiding infection bacterium, the source of disease bacterium such as such as haemophilus parasuis, Salmonella, streptococcus, baby swine paratyphoid bacterium, clostridieum welchii, escherichia coli, staphylococcus aureus, pleuropneumonia radiation bacillus, to provide the natural livestock product without medicine healthy aquaculture.
In order to reach antibacterial and antibacterial effect, reactive composition of plants proposed by the invention, mainly include Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and five kinds of medical materials of Radix Glycyrrhizae, its composition part by weight is Flos Lonicerae: Radix Isatidis: Radix Astragali: Fructus Crataegi: Radix Glycyrrhizae ﹦ 1～1.5:1～1.5:1～1.5:1～1.5:1～1.5, and be mixed into reactive composition of plants with this proportion of composing, wherein each medical material sequentially extracts rear concentrate drying generation person with water, ethanol and three kinds of solvents of ethyl acetate respectively.Be that Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae are respectively after the ethyl acetate of the water of too high polarity, semipolar ethanol and low polarity is extracted, reconcentration is dried and generates respectively Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract, then the extract of these medical materials is mixed into reactive composition of plants of the present invention with aforementioned proportion; Or be that after first Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae being mixed, then concentrate drying directly generates reactive composition of plants of the present invention after water, ethanol and three kinds of solvents of ethyl acetate extract.Moreover, no matter be first to extract rear mixing, still first mix afterwards and extract, all can pass through the particular combinations ratio of these medical materials, wherein, at above-mentioned reactive composition of plants, also can be added with the Radix Pulsatillae of 1～1.5 part by weight or Herba Scutellariae Barbatae or its extract of its extract and/or 1～1.5 part by weight, make the effect of this reactive composition of plants performance Synergistic, reach effect of bacteriostasis antibiosis.Make, in whole actual breeding process, all need not add any antibiotic, chemical bactericide or medicine, can reach the healthy meat without any drug residue.
Extraction is extracted in after the composition medical material that reactive composition of plants of the present invention has been described, connecting needle describes the needed extracting method of medical material, and coordinates graphic being described in detail as follows.
Shown in Fig. 1, first, as shown in step S10, provide respectively medical material Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae, to prepare to carry out the extraction procedure of medical material; Then start to carry out the first stage extraction of step S12～step S16, as shown in step S12, by medical material, taking Flos Lonicerae as example, use the water soaking of 3～10 times of its weight after 1～5 hour, first as shown in step S14, carry out for the first time reflux 1～5 hour, this for the first time reflux temperature can be 100 DEG C, again through as after cooling, the filtration step of step S16, to obtain respectively the first filtrate 10 and the first residue 12; Then as shown in step S18, this first filtrate 10 through be evaporated to volume originally 1/3rd after form the first concentrated solution 14.Then carrying out the second stage of step S20～S24 extracts, as shown in step S20, soak with ethanol by the first residue 12 taking 3～10 times of its weight and concentration as 50～95% is after 1～5 hour, the first reflux for the second time through step S22 is after 1～5 hour, this for the second time reflux temperature can be 78 DEG C, after cooling, the filtration step of step S24, to obtain respectively the second filtrate 16 and the second residue 18, this second filtrate 16 is reclaimed ethanol through the concentrating under reduced pressure of step S26 can form the second concentrated solution 20 after existing without ethanol.Then, the second residue 18 is proceeded the phase III of step S28～S32 again and is extracted, as shown in step S28, the second residue 18 is soaked 1～5 hour taking 3～10 times of its weight and concentration as 50～95% ethyl acetate, first pass through the reflux for the third time 1～5 hour of step S30, this for the third time reflux temperature can be 77 DEG C, again after cooling, the filtration step of step S32, to obtain the 3rd filtrate 22, this 3rd filtrate 22 is reclaimed ethyl acetate through step S34 concentrating under reduced pressure and form the 3rd concentrated solution 24 after being existed without ethyl acetate.Finally as shown in step S36 and step S38, aforementioned the first concentrated solution 14, the second concentrated solution 20 and the 3rd concentrated solution 24 sequentially obtaining first mixed and proceed to be afterwards dried, this drying steps can use Direct spraying to be dried or lyophilization, or be first to add to carry out again the dry or lyophilization of Direct spraying after 5～80% excipient of reactive composition of plants weight, to obtain the extract 26 of medical material, namely Flos Lonicerae extract.Proving by the same methods, the present invention can extract respectively Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract by the flow process of above-mentioned steps S10～step S38; After obtaining the extract of all medical materials, can, according to aforesaid part by weight, all medicinal substances extracts such as Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract be mixed into reactive composition of plants.
Moreover, if wish is added the Radix Pulsatillae or Herba Scutellariae Barbatae, or the two adds together, also extract respectively Radix Pulsatillae extract, Herba Scutellariae Barbatae extract by the flow process of above-mentioned steps S10～step S38, mix to scale with aforementioned various extracts more afterwards.
Certainly, the explanation of previous embodiment be first by each medical material respectively first with after water, ethanol and ethyl acetate solvent extraction after concentrate drying is generated, then be mixed into reactive composition of plants with above-mentioned part by weight.In addition, the present invention also can first mix Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae in advance with the part by weight of above-mentioned 1～1.5:1～1.5:1～1.5:1～1.5:1～1.5 after, again this mixing medical material is sequentially extracted to rear concentrate drying with water, ethanol and ethyl acetate solvent and directly extract reactive composition of plants, detailed extraction flow process is also identical with Fig. 1 and corresponding explanation thereof, therefore repeat no more in this.
Continue and test with actual experiment, to prove that the reactive composition of plants that the present invention uses has significant antibacterial or fungistatic effect really.
Test with paper ingots diffusion method (inhibition zone), first, be prepared with each group of test specimen, comprising:
(1) A group: first extract decomposite reactive composition of plants, the reactive composition of plants being mixed into the part by weight of 1.2:1.2:1.3:1.1:1.1 by Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract.
(2) B group: first mix the reactive composition of plants extracting again, after first Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae being mixed with the medical material of the part by weight of 1.2:1.2:1.3:1.1:1.1, again after aforementioned leaching process, the reactive composition of plants of acquisition.
(3) C group: A group+Radix Pulsatillae extract and Herba Scutellariae Barbatae extract, it is blended together and form with the part by weight of 1.2:1.2:1.3:1.1:1.1:1.2:1.2 by Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract, Radix Glycyrrhizae extract, Radix Pulsatillae extract and Herba Scutellariae Barbatae extract.
(4) D group: first by Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi, Radix Glycyrrhizae, the Radix Pulsatillae, Herba Scutellariae Barbatae, after mixing with the medical material of the part by weight of 1.2:1.2:1.3:1.1:1.1:1.2:1.2, again after aforementioned leaching process, the reactive composition of plants of acquisition.
(5) matched group: chloromycetin (chloramphenicol).
These three groups of test specimens are mixed with to the concentration of 100mg/ml.Prepare different agar-agar culture medium according to various strains such as haemophilus parasuis, Salmonella, streptococcus, baby swine paratyphoid bacterium, clostridieum welchii, escherichia coli, staphylococcus aureus, pleuropneumonia radiation bacillus; Source of disease bacteria microorganism inoculum concentration approximately 5 × 10
6clump count/milliliter (cfu/ml), 0.1ml/plate.Agar-agar paper ingots diffusion test paper ingots add the test specimen containing 20ul.Continue, in aseptic operating platform, put into containing the agar-agar that detects microorganism 0.1ml and cultivate based on culture plate, coating is even, then the paper ingots that add test specimen is put into the agar-agar dish of planting bacterium.According to temperature required source of disease bacteria growing 18～24 hours that allow, when the pastille composition in paper ingots is slowly diffused in agar-agar, can suppress microbial growth.Wherein, by the size of inhibition zone (Zone of inhibition), can know the antimicrobial intensity of test specimen in paper ingots, the inhibition zone size of observing each test specimen, inhibition zone is larger, and antimicrbial power is better.Result of the test is as shown in table 1 below.
Table 1 suppresses circle size unit: mm
By the result of the test of table 1, can find, in each group of strain, chloromycetin as a control group behaves oneself best, and has maximum inhibition zone, this be because of chloromycetin be a kind of common antibacterial class antibiotic; The reactive composition of plants of reviewing A group and B group, the inhibition zone of the two is identical or quite approaching, represents that the antibacterial effect of the two is suitable, although performance does not have matched group good, really still exists effectively antibacterial or fungistatic effect; In addition, add C group and the D group of the Radix Pulsatillae and Herba Scutellariae Barbatae in A group and B group, its antibacterial effect also approaches with A group and B group or is identical.Hence one can see that, concerning cultivation dealer, can use safer without anxiety, the cultivating condition of antibiotic-free has more meaning, therefore in breeding process, can directly replace antibiotic with reactive composition of plants, to improve the antibacterial or antibacterial immunity of animal in breeding process, and then reach the object person of the green cultivation.
In sum, the reactive composition of plants that the present invention proposes, mix to scale again after first extracting respectively no matter be, or row extraction again after first mixing to scale, the two all can effectively improve the immunity of animal, there is good antibacterial, fungistatic effect, and then the natural livestock product without medicine healthy aquaculture is provided.
Above-described embodiment is described the preferred embodiment of the present invention; not scope of the present invention is limited; design under the prerequisite of spirit not departing from the present invention; various distortion and improvement that those of ordinary skill in the art make technical scheme of the present invention, all should fall in the definite protection domain of the claims in the present invention book.
Claims (11)
1. a biocidal property reactive composition of plants, is characterized in that comprising the extract of following medical material: Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae;
By following two kinds of methods, any one prepares described reactive composition of plants:
First method: by Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae respectively first taking water, ethanol and ethyl acetate after solvent extracts, concentrate drying, obtain respectively Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract, the part by weight that is 1～1.5:1～1.5:1～1.5:1～1.5:1～1.5 by Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract according to weight ratio again mixes, and extracts and obtains described reactive composition of plants; Or
Second method: the part by weight that is 1～1.5:1～1.5:1～1.5:1～1.5:1～1.5 according to weight ratio by Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae mix again by mixed medical material respectively taking water, ethanol and ethyl acetate after solvent extracts, concentrate drying, obtains the reactive composition of plants that contains Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract.
2. biocidal property reactive composition of plants as claimed in claim 1, is characterized in that more including the Radix Pulsatillae or its extract of 1～1.5 part by weight.
3. biocidal property reactive composition of plants as claimed in claim 2, is characterized in that more including Herba Scutellariae Barbatae or its extract of 1～1.5 part by weight.
4. an extraction preparation method for biocidal property reactive composition of plants, is characterized in that any one prepares by following two kinds of methods:
First method:
Provide respectively following medical material: comprise Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae;
By each medical material respectively after the water soaking of 3～10 times with its weight, first, after reflux for the first time, sequentially, after supercooling, filtering, to obtain respectively the first filtrate and the first residue, this first filtrate forms the first concentrated solution after concentrating under reduced pressure;
By this first residue taking 3～10 times of its weight and concentration after 50～95% soak with ethanol, first after reflux for the second time, sequentially, after supercooling, filtering, to obtain respectively the second filtrate and the second residue, this second filtrate forms the second concentrated solution after concentrating under reduced pressure;
By this second residue taking 3～10 times of its weight and concentration after 50～95% ethyl acetate is soaked, first, after reflux for the third time, sequentially, after supercooling, filtering, to obtain the 3rd filtrate, the 3rd filtrate forms the 3rd concentrated solution after concentrating under reduced pressure; And
After being mixed, this first concentrated solution, this second concentrated solution and the 3rd concentrated solution be dried, to obtain the extract of each medical material, make respectively Flos Lonicerae extract, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract according to above method;
The Flos Lonicerae extract preparing, Radix Isatidis extract, Radix Astragali extract, Fructus Crataegi extract and Radix Glycyrrhizae extract are mixed in proportion and obtain reactive composition of plants;
Second method:
Provide respectively following medical material: comprise Flos Lonicerae, Radix Isatidis, Radix Astragali, Fructus Crataegi and Radix Glycyrrhizae, each medical material is mixed in proportion;
After the water soaking of 3～10 times by mixed medical material with its weight, first, after reflux for the first time, sequentially, after supercooling, filtering, to obtain respectively the first filtrate and the first residue, this first filtrate forms the first concentrated solution after concentrating under reduced pressure;
By this first residue taking 3～10 times of its weight and concentration after 50～95% soak with ethanol, first after reflux for the second time, sequentially, after supercooling, filtering, to obtain respectively the second filtrate and the second residue, this second filtrate forms the second concentrated solution after concentrating under reduced pressure;
By this second residue taking 3～10 times of its weight and concentration after 50～95% ethyl acetate is soaked, first, after reflux for the third time, sequentially, after supercooling, filtering, to obtain the 3rd filtrate, the 3rd filtrate forms the 3rd concentrated solution after concentrating under reduced pressure;
After this first concentrated solution, this second concentrated solution and the 3rd concentrated solution are mixed, be dried, obtain reactive composition of plants.
5. the extraction preparation method of biocidal property reactive composition of plants as claimed in claim 4, the ratio that the extract that it is characterized in that extracting this Flos Lonicerae, this Radix Isatidis, this Radix Astragali, this Fructus Crataegi and this Radix Glycyrrhizae in first method is is 1～1.5:1～1.5:1～1.5:1～1.5:1～1.5 according to weight ratio is mixed into this reactive composition of plants.
6. the extraction preparation method of biocidal property reactive composition of plants as claimed in claim 4, it is characterized in that in second method before carrying out water soaking, the ratio that is 1～1.5:1～1.5:1～1.5:1～1.5:1～1.5 according to weight ratio by this Flos Lonicerae, this Radix Isatidis, this Radix Astragali, this Fructus Crataegi and this Radix Glycyrrhizae is mixed.
7. the extraction preparation method of the biocidal property reactive composition of plants as described in claim 5 or 6, is characterized in that wherein this medical material more comprises that weight ratio is 1～1.5 the Radix Pulsatillae and/or Herba Scutellariae Barbatae.
8. the extraction preparation method of biocidal property reactive composition of plants as claimed in claim 4, is characterized in that the time that this water soaking, this soak with ethanol and this ethyl acetate are soaked is respectively 1～5 hour.
9. the extraction preparation method of biocidal property reactive composition of plants as claimed in claim 4, is characterized in that this reflux, reflux and the time of reflux is respectively 1～5 hour for the third time for the second time for the first time.
10. the extraction preparation method of biocidal property reactive composition of plants as claimed in claim 4, is characterized in that this is dried as Direct spraying is dry or lyophilization.
The extraction preparation method of 11. biocidal property reactive composition of plants as claimed in claim 10, is characterized in that: first add before this drying steps and carry out dry or this lyophilization of this Direct spraying after 5～80% excipient of reactive composition of plants weight again.
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| CN109128214A (en) * | 2018-09-03 | 2019-01-04 | 黄冈师范学院 | A kind of method that Radix Isatidis residue prepares copper nanoparticle |
| CN109200233A (en) * | 2018-10-26 | 2019-01-15 | 四川新绿色药业科技发展有限公司 | A kind of drug and preparation method thereof for treating pheretima bacteriosis |
| CN109498718A (en) * | 2018-12-07 | 2019-03-22 | 沈阳农业大学 | A kind of dedicated herbal mixture of broiler chicken and its manufacture craft |
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