CN104970093A - Composite fermented Chinese herbal medicine food preservative - Google Patents
Composite fermented Chinese herbal medicine food preservative Download PDFInfo
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- CN104970093A CN104970093A CN201510396078.4A CN201510396078A CN104970093A CN 104970093 A CN104970093 A CN 104970093A CN 201510396078 A CN201510396078 A CN 201510396078A CN 104970093 A CN104970093 A CN 104970093A
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Abstract
The present invention discloses a composite fermented Chinese herbal medicine food preservative which is natural, high efficient, safe, non-toxic, etc. The food preservative selects safe and side effect free Chinese herbal medicines of coptis chinensis, houttuynia cordata, cloves, scutellaria baicalensis, rheum palmatum, phellodendron chinense barks, herba taching, pulsatilla chinensis, forsythia suspensa, dandelions, herba artemisiae scopariae, licorice, etc. Ultrasonic assisted extraction and microbial fermentation combined fermentation technology combines the antimicrobial active ingredients in the composite Chinese herbal medicines, Bacillus subtilis, and various antibacterial substances in the mannan, seaweed polysaccharides, etc. together. The food preservative product has a high leaching rate of active ingredients of the Chinese herbal medicines, has a broad antimicrobial range and a strong antibacterial ability, can effectively extend food preservation duration, and meet people's demand for healthy, safe, side effect free food preservatives.
Description
Technical field
The present invention relates to a kind of Composite fermentation type Chinese herbal medicine food antistaling agent, belong to food additives field.
Background technology
Food processing industry has become one of large pillar industry of national economy three.Encroach on and spoilage because food is easy to be subject to various harmful microbe in the process of processing, storage, consumption, the fresh-keeping major issue just become in food processing industry of food antiseptic.Along with the progressively reinforcement of people's living standard and environmental consciousness, the requirement of people to antisepsis antistaling agent is also more and more higher.Food preservative technology has physics, chemistry and biological method.Physical method has microwave disinfection, high voltage electric field sterilization, high pressure low temperature preservation, electrostatic sterilization, magnetic force sterilization, radiation sterilization, infrared sterilization etc., and the physical method such as irradiation storage, cryopreservation is because of the restriction such as the scope of application, operating technology, be difficult to extensively promote.Chemical preservative such as nitrite, Sodium Benzoate etc. all have certain toxicity; And biological preservation has the features such as nontoxic, tasteless, safe.Therefore find the biological way of keeping fresh of safety non-toxic, become the focus of people's concern for substituted chemistry preservation method, researched and developed natural, efficient, nontoxic, safe food preservative imperative.
Due to Chinese herbal medicine, to have side effect little, and source is wide, cheap, lessly occurs the advantages such as drug resistance, makes from Chinese herbal medicine, screen the extensive attention that antibacterials are more and more subject to Chinese scholars.As far back as the 1950's, namely China medical personal starts the research of Chinese herbal medicine bacteriostasis and antipathogenic composition, and finds the Chinese herbal medicine of many tool antibacterial actions.The people such as Ru Wangyue, Wu Jun successively determine the antibacterial action of multiple Foochow herbal medicine.Current result of study display, much has heat-clearing, the Chinese herbal medicine of anti-inflammatory and removing toxic substances all has antibacterial action, for the exploitation of natural fruit vegetables antistaling agent provides possibility.
Summary of the invention
The object of the present invention is to provide a kind of Composite fermentation type Chinese herbal medicine food antistaling agent, this antistaling agent has the advantages such as natural, efficient, safe, nontoxic.
For achieving the above object, the technical solution used in the present invention is as follows:
A kind of Composite fermentation type Chinese herbal medicine food of the present invention antistaling agent, is characterized in that preparation technology comprises the following steps:
(1) Chinese herbal medicine pretreatment: sorting Chinese herbal medicine, removes moth and rotten person, cleaning, shearing-crushing;
(2) weigh: in the coptis 20 ~ 30 parts, cordate houttuynia 20 ~ 30 parts, cloves 18 ~ 22 parts, the root of large-flowered skullcap 15 ~ 20 parts, rheum officinale 10 ~ 15 parts, golden cypress 10 ~ 15 parts, folium isatidis 10 ~ 15 parts, the Chinese bulbul 10 ~ 12 parts, the capsule of weeping forsythia 8 ~ 12 parts, dandelion 6 ~ 10 parts, old 4 ~ 6 parts of bacterium, Radix Glycyrrhizae 3 ~ 5 parts of ratio precise herbal raw materials;
(3) be pulverized and mixed: mixing after herbal raw material is pulverized, cross 100 mesh sieves;
(4) Ultrasonic Wave-Assisted Extraction: add 5 ~ 8 times of pure water, and add 2 ~ 3% non-starch polysaccharide enzymes, under ultrasonic power 300W condition, 60 ~ 80 DEG C of water bath with thermostatic control refluxing extraction 40 ~ 60min;
(5) zymotic fluid preparation: add 2 ~ 3% glucose, 0.2 ~ 0.3% potassium dihydrogen phosphate, 0.1 ~ 0.2% magnesium sulfate in Chinese herbal medicine leaching liquor, regulates zymotic fluid pH6.5 ~ 7.0;
(6) fermentable: inoculate 2 ~ 3% bacillus subtilises in zymotic fluid, ventilating fermentation 6 ~ 12h under 28 ~ 32 DEG C of conditions;
(7) filter: filter under agitation after fermentation ends;
(8) composite: in the zymotic fluid after filtration, to add 1 ~ 2% mannosan, 3 ~ 5% algal polysaccharides;
(9) homogeneous: on homogenizer, namely Composite fermentation type Chinese herbal medicine food antistaling agent is obtained to zymotic fluid homogeneous.
Above-mentioned algal polysaccharides refers to one or more in polysaccharide from Dunaliella salina, laminarin, sea grass polysaccharide, sea lettuce polysaccharide.
The invention provides a kind of Composite fermentation type Chinese herbal medicine food antistaling agent, can effectively extend food fresh keeping duration, modes such as rinsing can being adopted after antistaling agent being diluted 10 ~ 100 times during use, spray, smear, convenient, fast, efficient.
The invention provides a kind of Composite fermentation type Chinese herbal medicine food antistaling agent, it is advantageous that: one be raw material choose safely, the Chinese herbal medicine such as old, the Radix Glycyrrhizae of the coptis that has no side effect, cordate houttuynia, cloves, the root of large-flowered skullcap, rheum officinale, golden cypress, folium isatidis, the Chinese bulbul, the capsule of weeping forsythia, dandelion, bacterium, meet people to healthy, safely, the demand of the food preservative that has no side effect; Two is zymotechniques that the Chinese herbal medicine with bacteriostasis has been carried out Ultrasonic Wave-Assisted Extraction and fermentable and combines by the present invention, improves the leaching of the fresh-keeping active component of Chinese herbal medicine, further increases the fungistatic effect of compound food fresh-keeping agent; Three is that the multiple Substances such as the bacteriostatic active ingredients of composite Chinese herbal, bacillus subtilis, mannosan, algal polysaccharides combine by the present invention, food preservative product scope of restraining fungi is wide, bacteriostasis strong, can effectively extend food fresh keeping duration.
The invention provides a kind of recycling scallop shell and prepare high-efficiency compound food antistaling agent, compatibility applies the coptis with bacteriostasis efficacy, cordate houttuynia, cloves, the root of large-flowered skullcap, rheum officinale, golden cypress, folium isatidis, the Chinese bulbul, the capsule of weeping forsythia, dandelion, bacterium is old, Radix Glycyrrhizae and bacillus subtilis, mannosan etc., both restraining and sterilizing bacteria effect of Chinese herbal medicine had fully been mixed, pass through bacillus subtilis again, the coupling of polysaccharide and Chinese herbal medicine Substance, extend the fresh keeping time of antistaling agent, particularly pass through fermentation of bacillus subtilis, improve the leaching of active component in Chinese herbal medicine, thus provide a kind of fresh keeping time long, protect effective fruit antistaling agent.Demonstrate this application by fruit freshness preserving test and achieve unforeseeable technique effect, to hold perishable honey peach for subjects, result of the test shows to use the effective freshness date of product of the present invention can reach more than 10 days, bacteriostasis rate reaches more than 99.3%, and comparatively chemical preservative control group fresh keeping time extends more than 8.5 days; Result of the test shows that being used alone Chinese herbal medicine leaching liquor or bacillus subtilis or algal polysaccharides Be very effective reduces, difference extremely significantly (P<0.01), change Chinese herbal medicine composition or ratio also significantly can reduce the safety effect of antistaling agent, and statistical discrepancy significantly (P<0.05).
Detailed description of the invention
embodiment 1:
A kind of Composite fermentation type Chinese herbal medicine food antistaling agent, is characterized in that comprising the following steps:
(1) Chinese herbal medicine pretreatment: sorting Chinese herbal medicine, removes moth and rotten person, cleaning, shearing-crushing;
(2) weigh: in the coptis 30 parts, cordate houttuynia 20 parts, cloves 22 parts, the root of large-flowered skullcap 15 parts, rheum officinale 15 parts, golden cypress 10 parts, folium isatidis 15 parts, the Chinese bulbul 10 parts, the capsule of weeping forsythia 12 parts, dandelion 6 parts, old 6 parts of bacterium, Radix Glycyrrhizae 3 parts of ratio precise herbal raw materials;
(3) be pulverized and mixed: mixing after herbal raw material is pulverized, cross 100 mesh sieves;
(4) Ultrasonic Wave-Assisted Extraction: add 8 times of pure water, and add 3% non-starch polysaccharide enzyme, under ultrasonic power 300W condition, 60 ~ 80 DEG C of water bath with thermostatic control refluxing extraction 40min;
(5) zymotic fluid preparation: add 3% glucose, 0.3% potassium dihydrogen phosphate, 0.2% magnesium sulfate in Chinese herbal medicine leaching liquor, regulates zymotic fluid pH6.5 ~ 7.0;
(6) fermentable: inoculate 3% bacillus subtilis in zymotic fluid, ventilating fermentation 6h under 28 ~ 32 DEG C of conditions;
(7) filter: filter under agitation after fermentation ends;
(8) composite: in the zymotic fluid after filtration, to add 2% mannosan, 5% algal polysaccharides;
(9) homogeneous: on homogenizer, namely Composite fermentation type Chinese herbal medicine food antistaling agent is obtained to zymotic fluid homogeneous.
embodiment 2:
A kind of Composite fermentation type Chinese herbal medicine food antistaling agent, is characterized in that comprising the following steps:
(1) Chinese herbal medicine pretreatment: sorting Chinese herbal medicine, removes moth and rotten person, cleaning, shearing-crushing;
(2) weigh: in the coptis 20 parts, cordate houttuynia 30 parts, cloves 18 parts, the root of large-flowered skullcap 20 parts, rheum officinale 10 parts, golden cypress 15 parts, folium isatidis 10 parts, the Chinese bulbul 12 parts, the capsule of weeping forsythia 8 parts, dandelion 10 parts, old 4 parts of bacterium, Radix Glycyrrhizae 5 parts of ratio precise herbal raw materials;
(3) be pulverized and mixed: mixing after herbal raw material is pulverized, cross 100 mesh sieves;
(4) Ultrasonic Wave-Assisted Extraction: add 5 ~ 8 times of pure water, and add 2 ~ 3% non-starch polysaccharide enzymes, under ultrasonic power 300W condition, 60 ~ 80 DEG C of water bath with thermostatic control refluxing extraction 40 ~ 60min;
(5) zymotic fluid preparation: add 2% glucose, 0.2% potassium dihydrogen phosphate, 0.1% magnesium sulfate in Chinese herbal medicine leaching liquor, regulates zymotic fluid pH6.5 ~ 7.0;
(6) fermentable: inoculate 2% bacillus subtilis in zymotic fluid, ventilating fermentation 12h under 28 ~ 32 DEG C of conditions;
(7) filter: filter under agitation after fermentation ends;
(8) composite: in the zymotic fluid after filtration, to add 1% mannosan, 3% algal polysaccharides;
(9) homogeneous: on homogenizer, namely Composite fermentation type Chinese herbal medicine food antistaling agent is obtained to zymotic fluid homogeneous.
embodiment 3:
A kind of Composite fermentation type Chinese herbal medicine food antistaling agent, is characterized in that comprising the following steps:
(1) Chinese herbal medicine pretreatment: sorting Chinese herbal medicine, removes moth and rotten person, cleaning, shearing-crushing;
(2) weigh: in the coptis 25 parts, cordate houttuynia 25 parts, cloves 20 parts, the root of large-flowered skullcap 18 parts, rheum officinale 13 parts, golden cypress 12 parts, folium isatidis 12 parts, the Chinese bulbul 11 parts, the capsule of weeping forsythia 10 parts, dandelion 8 parts, old 5 parts of bacterium, Radix Glycyrrhizae 4 parts of ratio precise herbal raw materials;
(3) be pulverized and mixed: mixing after herbal raw material is pulverized, cross 100 mesh sieves;
(4) Ultrasonic Wave-Assisted Extraction: add 6.5 times of pure water, and add 2.5% non-starch polysaccharide enzyme, under ultrasonic power 300W condition, 60 ~ 80 DEG C of water bath with thermostatic control refluxing extraction 50min;
(5) zymotic fluid preparation: add 2.5% glucose, 0.25% potassium dihydrogen phosphate, 0.15% magnesium sulfate in Chinese herbal medicine leaching liquor, regulates zymotic fluid pH6.5 ~ 7.0;
(6) fermentable: inoculate 2.5% bacillus subtilis in zymotic fluid, ventilating fermentation 9h under 28 ~ 32 DEG C of conditions;
(7) filter: filter under agitation after fermentation ends;
(8) composite: in the zymotic fluid after filtration, to add 1.5% mannosan, 4% algal polysaccharides;
(9) homogeneous: on homogenizer, namely Composite fermentation type Chinese herbal medicine food antistaling agent is obtained to zymotic fluid homogeneous.
Above embodiment only for illustration of technical scheme of the present invention, but not is limited; Although be described in detail by invention with reference to previous embodiment, for the person of ordinary skill of the art, still the technical scheme described in previous embodiment can be modified, or equivalent replacement has been carried out to wherein portion of techniques feature; And to these amendments or replacement, do not make the essence of appropriate technical solution depart from the spirit and scope of the present invention's technical scheme required for protection.
Claims (2)
1. a Composite fermentation type Chinese herbal medicine food antistaling agent, is characterized in that preparation technology comprises the following steps:
(1) Chinese herbal medicine pretreatment: sorting Chinese herbal medicine, removes moth and rotten person, cleaning, shearing-crushing;
(2) weigh: in the coptis 20 ~ 30 parts, cordate houttuynia 20 ~ 30 parts, cloves 18 ~ 22 parts, the root of large-flowered skullcap 15 ~ 20 parts, rheum officinale 10 ~ 15 parts, golden cypress 10 ~ 15 parts, folium isatidis 10 ~ 15 parts, the Chinese bulbul 10 ~ 12 parts, the capsule of weeping forsythia 8 ~ 12 parts, dandelion 6 ~ 10 parts, old 4 ~ 6 parts of bacterium, Radix Glycyrrhizae 3 ~ 5 parts of ratio precise herbal raw materials;
(3) be pulverized and mixed: mixing after herbal raw material is pulverized, cross 100 mesh sieves;
(4) Ultrasonic Wave-Assisted Extraction: add 5 ~ 8 times of pure water, and add 2 ~ 3% non-starch polysaccharide enzymes, under ultrasonic power 300W condition, 60 ~ 80 DEG C of water bath with thermostatic control refluxing extraction 40 ~ 60min;
(5) zymotic fluid preparation: add 2 ~ 3% glucose, 0.2 ~ 0.3% potassium dihydrogen phosphate, 0.1 ~ 0.2% magnesium sulfate in Chinese herbal medicine leaching liquor, regulates zymotic fluid pH6.5 ~ 7.0;
(6) fermentable: inoculate 2 ~ 3% bacillus subtilises in zymotic fluid, ventilating fermentation 6 ~ 12h under 28 ~ 32 DEG C of conditions;
(7) filter: filter under agitation after fermentation ends;
(8) composite: in the zymotic fluid after filtration, to add 1 ~ 2% mannosan, 3 ~ 5% algal polysaccharides;
(9) homogeneous: on homogenizer, namely Composite fermentation type Chinese herbal medicine food antistaling agent is obtained to zymotic fluid homogeneous.
2. a kind of Composite fermentation type Chinese herbal medicine food antistaling agent according to claim 1, is characterized in that one or more that described algal polysaccharides refers in polysaccharide from Dunaliella salina, laminarin, sea grass polysaccharide, sea lettuce polysaccharide.
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Cited By (14)
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| CN105248625A (en) * | 2015-10-23 | 2016-01-20 | 广西农垦国有立新农场 | Fresh keeping method for citruses |
| CN105660840A (en) * | 2016-01-20 | 2016-06-15 | 黄庆辉 | Chinese herbal medicine vegetable-fruit preservative and preservation method |
| CN105918424A (en) * | 2016-04-22 | 2016-09-07 | 柳州蓓蒂芬科技有限公司 | Fresh-keeping agent for aquatic products |
| CN106490139A (en) * | 2016-10-14 | 2017-03-15 | 吴迪 | A kind of preparation method of natural fresh-keeping agent of fruits |
| CN107251941A (en) * | 2017-07-10 | 2017-10-17 | 桂林融通科技有限公司 | A kind of preparation method of antistaling agent for litchi |
| CN107549289A (en) * | 2017-07-20 | 2018-01-09 | 通化师范学院 | A kind of Chinese herbal medicine preservative of the Salvelinus malma flesh of fish and application thereof |
| CN107771926A (en) * | 2017-12-13 | 2018-03-09 | 柳州市柳科科技有限公司 | A kind of preparation method of antistaling agent for litchi |
| CN107811032A (en) * | 2017-09-27 | 2018-03-20 | 铜仁市万山区青蕴农业生态园有限公司 | A kind of method stored for a long time suitable for cordate houttuynia |
| CN107836514A (en) * | 2017-11-29 | 2018-03-27 | 青岛嘉瑞生物技术有限公司 | A kind of winter jujube tailored version efficient fresh-keeping agent and preparation method thereof |
| CN107873823A (en) * | 2017-12-13 | 2018-04-06 | 柳州市柳科科技有限公司 | A kind of preservation method of lichee |
| CN107969484A (en) * | 2017-12-13 | 2018-05-01 | 柳州市柳科科技有限公司 | A kind of preparation method of antistaling agent for longan |
| CN108056159A (en) * | 2017-12-13 | 2018-05-22 | 柳州市柳科科技有限公司 | A kind of preservation method of longan |
| WO2019194758A1 (en) * | 2018-04-07 | 2019-10-10 | Ucar Dilek | Food protecting composition for meat, meat products and dairy products |
| CN110468159A (en) * | 2019-07-05 | 2019-11-19 | 闵缘生物科技(浙江)有限公司 | A kind of method and the antibiotic extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic |
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| CN105918424A (en) * | 2016-04-22 | 2016-09-07 | 柳州蓓蒂芬科技有限公司 | Fresh-keeping agent for aquatic products |
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| CN107836514A (en) * | 2017-11-29 | 2018-03-27 | 青岛嘉瑞生物技术有限公司 | A kind of winter jujube tailored version efficient fresh-keeping agent and preparation method thereof |
| CN107771926A (en) * | 2017-12-13 | 2018-03-09 | 柳州市柳科科技有限公司 | A kind of preparation method of antistaling agent for litchi |
| CN107873823A (en) * | 2017-12-13 | 2018-04-06 | 柳州市柳科科技有限公司 | A kind of preservation method of lichee |
| CN107969484A (en) * | 2017-12-13 | 2018-05-01 | 柳州市柳科科技有限公司 | A kind of preparation method of antistaling agent for longan |
| CN108056159A (en) * | 2017-12-13 | 2018-05-22 | 柳州市柳科科技有限公司 | A kind of preservation method of longan |
| WO2019194758A1 (en) * | 2018-04-07 | 2019-10-10 | Ucar Dilek | Food protecting composition for meat, meat products and dairy products |
| CN112218536A (en) * | 2018-04-07 | 2021-01-12 | 优卡健康有限公司 | Food protective composition for meat, meat products and dairy products |
| CN110468159A (en) * | 2019-07-05 | 2019-11-19 | 闵缘生物科技(浙江)有限公司 | A kind of method and the antibiotic extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic |
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