CN102047951A - Biological antistaling agent for peaches as well as preparation method and application thereof - Google Patents
Biological antistaling agent for peaches as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN102047951A CN102047951A CN2009102097871A CN200910209787A CN102047951A CN 102047951 A CN102047951 A CN 102047951A CN 2009102097871 A CN2009102097871 A CN 2009102097871A CN 200910209787 A CN200910209787 A CN 200910209787A CN 102047951 A CN102047951 A CN 102047951A
- Authority
- CN
- China
- Prior art keywords
- peach
- preservative
- antistaling agent
- bio
- bacillus subtilis
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a biological antistaling agent for peaches, comprising 995 to 999 parts of metabolic product of bacillus subtilis and 1 to 5 parts of Tween 20; and the thallus concentration is 1*10<8> to 1*10<9> pieces per mL. The method for preparing the antistaling agent comprises the following steps: activating antagonism strains, fermenting antagonism strains in a shaking table, removing thallus centrifugally, detecting samples and packaging; and the application of the antistaling agent comprises the processes of peach sorting, atomizing, air-drying and boxing. The antistaling agent provided by the invention is suitable for retaining freshness of peaches and other stone fruits in storing and transporting processes. Furthermore, the antistaling agent inhibits bacteria, prevents rot and brown stain, prolongs the aging process of peaches, and has better effect on keeping the freshness of honey peaches. The components of the antistaling agent are secretions of microorganisms rather than microorganism cells, thus the antistaling agent has the advantages of high safety, simple preparation process and low cost and is easy in industrial production, and the application of the antistaling agent is simple and convenient and is beneficial to popularization.
Description
Technical field
The present invention relates to a kind of fruit antistaling agent, be specifically related to a kind of bio-preservative that is used for peach and its production and application, belong to the technical field of fruit antistaling agent.
Background technology:
How sweet peach is, nutritious and liked by consumers in general with its juice.But the pulp matter of peach is soft, water content and sugar content height, and is very easily rotten; Picking time, the ripe senescence process of fruit harvesting was very fast, not anti-storage of the utmost point and transportation on the occasion of high temperature season.
The anti-corrosive fresh-keeping measure of peach employing at present mainly contains: ice pit storage, heat shock processing, electronic technology fresh-keeping method, microwave fresh-keeping method, spontaneous controlled-atmosphere method, subatmospheric pressure storage, use chemical bactericide or the like.But there is such or such shortcoming in present prior art:
The freezer storage is very easily damaged to plants caused by sudden drop in temperature, and condition is wayward;
Heat shock processing, electronic technology fresh-keeping method and microwave fresh-keeping method only are in conceptual phase at present, have a lot of key problem in technology not solve, and fail to enter production application;
Air conditioned storage is that effect is relative better with subatmospheric pressure storage, but cost is higher;
Use chemical bactericide can produce medicament residue, have a strong impact on consumer health and products export.
Therefore, the peach post-harvest fresh-keeping is badly in need of a kind of preservation technique with simple to operate, high effect nontoxic, natural safety and lower-price characteristic.
Summary of the invention
The present invention is exactly in order to address the above problem, and overcomes fruit growth of pathogenic bacteria, the breeding of peach, prevents rotten, brown stain in the peach storage process, delays the problem of peach aging course, and a kind of bio-preservative that is used for peach and its production and application is provided.This invention is applicable to peach, is particularly useful for honey peach, also can be used for other fruits and vegetables.
The present invention is in order to solve above-mentioned the deficiencies in the prior art part, a kind of efficient, safe, cheap bio-preservative to be provided.The present invention is that the metabolite of the food microorganisms [bacillus subtilis (Bacillus subtilis), by Canadian Agriculture And Agri-Food Canada, the food research center provides] that separate from bean product is made, and is safe.In addition, it is secretion rather than the microbial cell itself of microorganism, thereby has high security.This microbial life power is strong, and fast growth is less demanding to growth conditions, can grow in simple culture medium, and cost is low.
The technical problem that will solve required for the present invention can be achieved through the following technical solutions:
As first fermentation of the present invention, a kind of bio-preservative that is used for peach is characterized in that, comprises according to the parts by weight meter metabolite 995-999 part of microorganisms, polysorbas20 1-5 part; Described microorganism is bacillus subtilis (Bacillus subtilis), the cell concentration 1 * 10 of described microorganism
8～1 * 10
9Individual/ml.
The described bio-preservative that is used for peach comprises: metabolite 999 weight portions of microorganisms, polysorbas20 is 1 weight portion.
Described microorganism is bacillus subtilis (Bacillus subtilis), and this bio-preservative source is new, and the present invention is the metabolite that utilizes bacillus subtilis (Bacillus subtilis).
The used culture medium of the present invention is the LB culture medium of improvement, and the isolated effective food microorganisms from the soybean product of fermentation by dilution method of falling the ware and plate streak (both are in conjunction with using) utilize dull and stereotyped face-off method screening antagonistic strain behind the purifying.
Above-mentioned improvement LB culture medium prescription: glucose 5g/L, peptone 10g/L, yeast extract 5g/L, sodium chloride 5g/L, potassium dihydrogen phosphate 0.25g/L, calcium carbonate 0.25g/L, agar 18g/L, water 1L, pH7.0.
Flat band method is fallen in above-mentioned dilution: earlier material to be separated is made a series of 10 times of dilutions (as 1: 10,1: 100,1: 1000,1: 10000...) successively with sterilized water, drawing the 1mL dilution from each concentration gradient respectively then adds the sterilized culture dish, pour into again and dissolved and be cooled to improvement LB culture medium about 55 ℃, mix, after treating that agar solidifies, cultivate 24h for 30 ℃.The single bacterium colony that picking disperses in planar surface or agar medium, or repeat above operation for several times, just can obtain pure culture.
Above-mentioned plate streaking partition method: pick a little material to be separated with the bacterium operation by oese, carry out parallel scribing or fan-shaped line at aseptic planar surface, microbial cell quantity will reduce along with the increase of line number of times, and progressively spread out, if line is suitable, microorganism can disperse one by one, after cultivating, can obtain single bacterium colony at planar surface.
Above-mentioned dull and stereotyped face-off method: on solid PDA culture medium, middle inoculation plate streak purifying obtains and the bacterium piece of cultured diameter 0.5cm, both sides inoculation diameter is the pathogen of 0.5cm, cultivated 3-6 days for 28 ℃, measure every day 3 times pathogen diameter and antagonism bacterium diameter, inoculation aseptic culture medium piece in the middle of the contrast is by measuring the good bacterial strain of its inhibition ability screening antagonism performance.
As a second aspect of the present invention, a kind of preparation method who is used for the bio-preservative of peach is characterized in that, may further comprise the steps:
(1) activation of antagonism bacterium: bacterium solid culture medium is done the test tube slant, antagonistic strain bacillus subtilis (Bacillus subtilis) switching is enlarged on the inclined-plane cultivate;
(2) bacillus subtilis (Bacillus subtilis) antagonism bacterium shaker fermentation: thalline is forwarded to shaker fermentation in the fluid nutrient medium;
(3) bactofugation body: the bacterium liquid low-speed centrifugal that shaker fermentation is good is removed thalline, preserves supernatant with standby;
(4) sample detection: measure the spore germination inhibiting rate of fermented supernatant fluid to pathogen;
(5) packing: carry out plastic bottle packing by every bottle of 1000ml specification requirement.
Wherein, bacterium solid culture medium described in the step (1) is the test tube slant made of LB culture medium of improvement, with antagonistic strain bacillus subtilis (Bacillus subtilis) switching on the inclined-plane 28～30 ℃ cultivated 24～48 hours.
Wherein, improvement LB culture medium prescription: glucose 5g/L, peptone 10g/L, yeast extract 5g/L, sodium chloride 5g/L, potassium dihydrogen phosphate 0.25g/L, calcium carbonate 0.25g/L, agar 18g/L, water 1L, pH7.0.
Wherein, fermentation medium is the potato fluid nutrient medium in the step (2), and fermentation parameter is 115 rev/mins of shaking tables, 22～25 ℃ of temperature, fermentation time 7-10 days, cell concentration 1 * 10
8～1 * 10
9Individual/ml.
Wherein, centrifugal described in the step (3) is under 4500 rev/mins of conditions centrifugal 30 minutes, and the temperature of preserving supernatant is 4 ℃.
As a third aspect of the present invention, a kind of application that is used for the bio-preservative of peach is characterized in that, may further comprise the steps:
(1) peach letter sorting: select no disease and pest, have no mechanical damage, eight fens ripe peaches of 150～250g;
(2) spraying is handled: prepared preservative is sprayed on the peach surface uniformly;
(3) air-dry: the peach that spraying was handled dries up or natural air drying with fan;
(4) vanning, precooling treatment, preservation: should be equipped with in the antistaling box of individual layer crisper through air-dry peach, and avoid extruding; Be placed on precooling in the freezer earlier, again temperature of ice house be transferred to the peach reserve temperature.
Wherein, the described spraying of step (2) is handled, and before using antistaling agent and polysorbas20 is mixed with 1000: 1 solution, pore atomizer spray processing peach, each peach 5ml antistaling agent.
Wherein, 6～10 ℃ of the described precooling temperatures of step (4), humidity 85%～90%, pre-cool time 12-14 hour, 0～1 ℃ of described peach reserve temperature.
Beneficial effect of the present invention:
This bio-preservative source is new, the present invention be from the fermentation soybean product isolated food microorganisms as antagonistic strain, described microorganism is bacillus subtilis (Bacillus subtilis), the present invention is the metabolite that utilizes bacillus subtilis (Bacillus subtilis), does not add other chemical preservation compositions.Because this antistaling agent is not microorganism itself, but the metabolite of microorganism can decompose behind the certain hour automatically, more makes this antistaling agent environmental protection, pollution-free, noresidue to have guaranteed the security of its fresh-keeping foodstuff.
The present invention has and prevents that peach from rotting, keeping moisture, delaying the effect of its aging, and its fresh-keeping effect is apparently higher than other domestic bio-preservatives.
Antistaling agent preparation technology advanced person of the present invention, workable.
In addition, application process of the present invention is easy, good refreshing effect, and product cost is low, is easy to industrialization production and popularization.
Description of drawings
Further specify the present invention below in conjunction with the drawings and specific embodiments.
10 ℃ of following antistaling agents of Fig. 1 are to the peach influence of fruit rate well
25 ℃ of following antistaling agents of Fig. 2 are to the peach influence of fruit rate well
The specific embodiment
In order to make technological means of the present invention, creation characteristic, to reach purpose and effect is easy to understand,, further set forth the present invention below in conjunction with concrete diagram.
The source of bacterial classification and preparation.
The present invention is made by the metabolite of the food microorganisms that separate in the bean product [bacillus subtilis (Bacillussubtilis), by Canadian Agriculture And Agri-Food Canada, the food research center provides].
Microorganism is bacillus subtilis (Bacillus subtilis), the used culture medium of the present invention is the LB culture medium of improvement, the isolated effective food microorganisms from the soybean product of fermentation by dilution method of falling the ware and plate streak (both are in conjunction with using) utilize dull and stereotyped face-off method screening antagonistic strain behind the purifying.
Wherein, improvement LB culture medium prescription: glucose 5g/L, peptone 10g/L, yeast extract 5g/L, sodium chloride 5g/L, potassium dihydrogen phosphate 0.25g/L, calcium carbonate 0.25g/L, agar 18g/L, water 1L, pH7.0.
Wherein, dilution is flat band method: earlier material to be separated is made a series of 10 times of dilutions (as 1: 10,1: 100,1: 1000,1: 10000...) successively with sterilized water, drawing the 1mL dilution from each concentration gradient respectively then adds the sterilized culture dish, pour into again and dissolved and be cooled to improvement LB culture medium about 55 ℃, mix, after treating that agar solidifies, cultivate 24h for 28 ℃.The single bacterium colony that picking disperses in planar surface or agar medium, or repeat above operation for several times, just can obtain pure culture.
Wherein, plate streaking partition method: pick a little material to be separated with the bacterium operation by oese, carry out parallel scribing or fan-shaped line at aseptic planar surface, microbial cell quantity will reduce along with the increase of line number of times, and progressively spread out, if line is suitable, microorganism can disperse one by one, after cultivating, can obtain single bacterium colony at planar surface.
Wherein, dull and stereotyped face-off method: on solid PDA culture medium, middle inoculation plate streak purifying obtains and the bacterium piece of cultured diameter 0.5cm, both sides inoculation diameter is the pathogen of 0.5cm, cultivated 3-6 days for 28 ℃, every day, 3 mensuration pathogen diameter and antagonism bacterium diameter were inoculated the aseptic culture medium piece in the middle of the contrast, by measuring the good bacterial strain of its inhibition ability screening antagonism performance.
A kind of preparation method who is used for the bio-preservative of peach comprises following operating procedure:
(1) activation of antagonism bacterium: the inoculation that picking screens is at LB culture medium (the glucose 5g/L of improvement, peptone 10g/L, yeast extract 5g/L, sodium chloride 5g/L, potassium dihydrogen phosphate 0.25g/L, calcium carbonate 0.25g/L, agar 18g/L, water 1L is on test tube slant pH7.0), cultivated 24～48 hours for 28～30 ℃, stand-by;
(2) antagonism bacterium shaker fermentation: the antagonism bacterium that will activate is seeded in the potato fluid nutrient medium, 115～130 rev/mins of shaking tables, 22～25 ℃ of temperature, fermentation time 7-10 days, cell concentration 1 * 10
8～1 * 10
9Individual/mL;
(3) bactofugation body: zymotic fluid was removed thalline in centrifugal 30 minutes with 4500 rev/mins rotating speed, preserved supernatant with standby for 4 ℃;
(4) sample detection: measure the inhibiting rate that supernatant is sprouted spore germination or the silk nuclear of pathogen, to determine stability of sample;
(5) packing: carry out plastic bottle packing by every bottle of 1000ml specification requirement.
A kind of application that is used for the bio-preservative of peach comprises following operating procedure:
(1) peach letter sorting: select no disease and pest, have no mechanical damage, eight fens ripe peaches of 150～250g;
(2) spraying is handled: before using antistaling agent and polysorbas20 be mixed with 1000: 1 solution, the solution for preparing be sprayed on the peach surface uniformly with the pore sprayer, each peach 5mL;
(3) air-dry: the peach that spraying was handled dries up or natural air drying with fan;
(4) vanning, precooling treatment, preservation: should be equipped with through air-dry peach in the antistaling box of individual layer crisper, avoid extruding, be placed in the freezer of 6～10 ℃ of temperature, humidity 85%～90% precooling 12～14 hours, and again temperature of ice house be transferred to 0～1 ℃ of peach reserve temperature.
Above-mentioned preparation and application process should be noted: the supernatant of centrifugal mistake is wanted 4 ℃ of preservations; During application, it is even, complete that spraying is wanted, and each position of peach all will be sprayed onto.
Below be the comparative result of the present invention and other antistaling agents:
As shown in Figure 1,10 ℃ of following antistaling agents are to the peach influence of fruit rate well
As shown in Figure 2,25 ℃ of following antistaling agents are to the peach influence of fruit rate well
Annotate: C06 is a peach bio-preservative of the present invention, and B01 is the bigger biological antistaling agent for garden stuff of domestic another family's use amount.
Result of the test shows that referring to Fig. 1 and Fig. 2, the fresh-keeping effect of the present invention under normal temperature and cryogenic conditions is apparently higher than bio-preservative B01, and every indexs such as taste such as its good fruit rate, sense organ all are better than bio-preservative B01.
The invention belongs to bio-preservative, this bio-preservative is to utilize the LB culture medium of improvement by dilution method of falling the ware and plate streak, the metabolite that isolated food microorganisms produce from bean product, food microorganisms with anti-corrosion function do not add other chemical preservation compositions.Be particularly suitable for to stone fruits such as peach the storage and transportation in fresh-keeping.This bio-preservative be a kind of it can suppress growth, the breeding of the The main pathogenic fungi of peach, prevent rotting in the peach storage process, brown stain, delay the peach aging course, it is fresh-keeping to be used for honey peach, effect is better.Because this antistaling agent composition is secretion rather than the microbial cell itself of microorganism, thereby has high security.Its antistaling agent source is new, safety non-toxic, harmless, pollution-free, and simple, with low cost, the easy industrialization production of preparation technology, its application process is also comparatively easy, is beneficial to popularization.
More than show and described basic principle of the present invention, principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that describes in the foregoing description and the specification just illustrates principle of the present invention; the present invention also has various changes and modifications without departing from the spirit and scope of the present invention, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (8)
1. a bio-preservative that is used for peach is characterized in that, comprises according to the parts by weight meter metabolite 995-999 part of microorganisms, polysorbas20 1-5 part; Described microorganism is bacillus subtilis (Bacillus subtilis), the cell concentration 1 * 10 of described microorganism
8～1 * 10
9Individual/ml.
2. a kind of bio-preservative that is used for peach according to claim 1 is characterized in that, the described bio-preservative that is used for peach comprises: metabolite 999 weight portions of microorganisms, polysorbas20 is 1 weight portion.
3. a preparation method who is used for the bio-preservative of peach as claimed in claim 1 is characterized in that, may further comprise the steps:
(1) activation of antagonism bacterium: bacterium solid culture medium is done the test tube slant, antagonistic strain bacillus subtilis (Bacillus subtilis) switching is enlarged on the inclined-plane cultivate;
(2) bacillus subtilis (Bacillus subtilis) antagonism bacterium shaker fermentation: thalline is forwarded to shaker fermentation in the fluid nutrient medium;
(3) bactofugation body: the bacterium liquid low-speed centrifugal that shaker fermentation is good is removed thalline, preserves supernatant with standby;
(4) sample detection: measure the spore germination inhibiting rate of fermented supernatant fluid to pathogen;
(5) packing: carry out plastic bottle packing by every bottle of 1000ml specification requirement.
4. a kind of preparation method who is used for the bio-preservative of peach according to claim 3 is characterized in that, fermentation medium is the potato fluid nutrient medium in the step (2), fermentation parameter is 115 rev/mins of shaking tables, 22～25 ℃ of temperature, fermentation time 7-10 days, cell concentration 1 * 10
8～1 * 10
9Individual/ml.
5. a kind of preparation method who is used for the bio-preservative of peach according to claim 3 is characterized in that, centrifugal described in the step (3) is under 4500 rev/mins of conditions centrifugal 30 minutes, and the temperature of preserving supernatant is 4 ℃.
6. an application that is used for the bio-preservative of peach as claimed in claim 1 is characterized in that, may further comprise the steps:
(1) peach letter sorting: select no disease and pest, have no mechanical damage, eight fens ripe peaches of 150～250g;
(2) spraying is handled: prepared preservative is sprayed on the peach surface uniformly;
(3) air-dry: the peach that spraying was handled dries up or natural air drying with fan;
(4) vanning, precooling treatment, preservation: should be equipped with in the antistaling box of individual layer crisper through air-dry peach, and avoid extruding; Be placed on precooling in the freezer earlier, again temperature of ice house be transferred to the peach reserve temperature.
7. a kind of application that is used for the bio-preservative of peach according to claim 6, it is characterized in that the described spraying of step (2) is handled, before using antistaling agent and polysorbas20 be mixed with 1000: 1 solution, the pore atomizer spray is handled peach, each peach 5ml antistaling agent.
8. a kind of application that is used for the bio-preservative of peach according to claim 6 is characterized in that, 6～10 ℃ of the described precooling temperatures of step (4), humidity 85%～90%, pre-cool time 12-14 hour, 0～1 ℃ of described peach reserve temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102097871A CN102047951A (en) | 2009-10-29 | 2009-10-29 | Biological antistaling agent for peaches as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102097871A CN102047951A (en) | 2009-10-29 | 2009-10-29 | Biological antistaling agent for peaches as well as preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN102047951A true CN102047951A (en) | 2011-05-11 |
Family
ID=43953021
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009102097871A Pending CN102047951A (en) | 2009-10-29 | 2009-10-29 | Biological antistaling agent for peaches as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102047951A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103431027A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Fresh-keeping method for prolonging preservation duration of strawberries |
| CN104970093A (en) * | 2015-07-08 | 2015-10-14 | 青岛嘉瑞生物技术有限公司 | Composite fermented Chinese herbal medicine food preservative |
| CN106720273A (en) * | 2016-12-23 | 2017-05-31 | 湖南省土壤肥料研究所 | A kind of biological preservation conditioner |
-
2009
- 2009-10-29 CN CN2009102097871A patent/CN102047951A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103431027A (en) * | 2013-08-08 | 2013-12-11 | 河南科技大学 | Fresh-keeping method for prolonging preservation duration of strawberries |
| CN104970093A (en) * | 2015-07-08 | 2015-10-14 | 青岛嘉瑞生物技术有限公司 | Composite fermented Chinese herbal medicine food preservative |
| CN106720273A (en) * | 2016-12-23 | 2017-05-31 | 湖南省土壤肥料研究所 | A kind of biological preservation conditioner |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1372384B1 (en) | A novel antagonistic yeast useful in controlling spoilage of agricultural produce, methods of use thereof and compositions containing same | |
| RU2598270C2 (en) | New lactic acid bacteria and method for production of silage or fermented fodder with its application | |
| CN107988108B (en) | Mold-resistant bacillus and antibacterial application thereof | |
| CN104694430A (en) | Bacillus amyloliquefaciens subsp. plantarum ZFH-3 and application thereof | |
| Mercier et al. | Effect of wound moisture on the biocontrol by Candida oleophila of gray mold rot (Botrytis cinerea) of apple | |
| CN101914459B (en) | Peach fruit disease biological antiseptic preservative and application and used cryptococcus laurentii | |
| CN109479953A (en) | A method of the antimicrobial emulsion fresh-keeping for romaine lettuce and prepare romaine lettuce preservative film | |
| CN103315058B (en) | Application of leuconostoc mesenteroides strain | |
| CN104686196A (en) | Method for preserving and separating toadstool strain through sporocarp dried in shade | |
| CN104146058B (en) | A kind of control red bayberry adopt after the compelx coating fresh keeping method of corrupt and anthocyanogen degraded | |
| CN102246850B (en) | Biological preservative for oranges | |
| CN104017748B (en) | Endogeny biocontrol strain as well as preparation method and application of biocontrol bacterial agent prepared from endogeny biocontrol strain | |
| CN102047951A (en) | Biological antistaling agent for peaches as well as preparation method and application thereof | |
| CN112481141B (en) | Debaryomyces hansenii strain and application thereof in preparation of fruit preservative | |
| CN113729075A (en) | Method for controlling postharvest diseases and storing and refreshing cherry tomatoes by pichia caribbica | |
| CN102860356A (en) | Leechee biological fresh-keeping agent and preparation method thereof | |
| CN110628655B (en) | Yeast Lg-3 for controlling postharvest diseases of fruits and vegetables and using method thereof | |
| CN103125590A (en) | Grape biological fresh-keeping agent and preparation method thereof | |
| CN106912587A (en) | A kind of food refrigerating and fresh-keeping method | |
| CN103843880B (en) | Based on the biological preservation liquid and uses thereof of GABA in conjunction with bio-control yeast | |
| CN102197842B (en) | Method for controlling apple patulin | |
| CN110343644B (en) | Microbial inoculum and culture method and application thereof | |
| CN109380502B (en) | Blackberry fruit bacteriostatic preservative and blackberry fruit preservation method | |
| CN103320339B (en) | Leuconostoc mesenteroides strain | |
| Rahman et al. | Nutritional profile study of oyster mushroom (Pleurotus ostreatus) at Different Storage Conditions |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20110511 |