CN110468159A - A kind of method and the antibiotic extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic - Google Patents
A kind of method and the antibiotic extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic Download PDFInfo
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Abstract
The present invention relates to antibacterial anti-inflammatory technical fields, disclose method and the antibiotic that a kind of ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, include the following steps, natural strain obtains fermenting microbe after system ripe mashed potatoes mixed fermentation 48 hours, again plus clear water dilutes 20 times and is transplanted to tealeaves substrate, it stirs evenly and is put into togerther in fermentor with tealeaves substrate, it is sealed, it is kept for ferment at constant temperature 3 hours, then ultrasonic extraction and separation of solid and liquid are carried out, settling tank is placed into precipitate 24 hours, take supernatant liquor, filtering supernatant liquor obtains clear liquid, finally carry out the processing of guaranteeing the quality that sterilizes, after adding the freeze-drying of national standard preservative, obtain broad-spectrum antibiotic；The mass ratio of tealeaves substrate is tealeaves: radix scutellariae: Fructus Forsythiae: water: international preservative=4:2:2:92:0.3.The method and the antibiotic solve the problems such as bacterial drug resistance and achieve the effect that level of the new antibiotic as hormone without antibiotic and hormone side effect in multiple antibacterial fields.
Description
Technical field
The present invention relates to antibacterial anti-inflammatory technical fields more particularly to a kind of extraction enzymatic hydrolysis natural plants to prepare broad-spectrum antibiotic
Method and the antibiotic.
Background technique
Very severe, bacterium have widely used chemobiotic to the drug resistance situation of chemobiotic mostly at present
Higher resistant rate, it is resistance in the bacterium detected in 100 Yu Jia hospitals at home according to the data of China's bacterial resistance detection net
Medicine rate is all 50% or more, and more than so the superbacteria that most antibacterials cannot all kill also occurs successively, research and development
The speed of new antibiotic has much been unable to catch up with the step of bacterial resistance, how to research and develop safe and efficient not bacterial drug resistance
Antibiotic of new generation is imperative, is development trend in recent years by the high-efficiency antimicrobial element research and development of extracted form natural plant.
The utility value and economic benefit of tealeaves are only that the tender shoots of tea tree, and are accounted for by old leaf that tea grower discards
90% or more, since the purposes of tealeaves is single along with yield has closely been saturated, the competition of tea product necessarily causes the economy of tea grower
Benefit Drops, and tealeaves is almost a blank sheet of paper in the application of high-tech area, many important components in tealeaves mainly collect
In in aged tea leaf, discarded aged tea leaf is carried out deep processing by Applied Biotechnology can not only turn waste into wealth, and economic value
Much surmount ten times hundred times of tea tree tender shoots.
The method of existing extracted form natural plant antibiotic is mostly ethanol refluxing process, decocting method and ion-exchange-resin process
Deng, but efficiency low yield is few；Chemobiotic killing pathogenic bacteria targeting is single, and pathogenic bacteria have drug resistance to chemobiotic
And chemobiotic can generate toxic side effect.
Summary of the invention
It is an object of the invention to solve above-mentioned technical problem, the technical solution adopted is that:
A kind of method that ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, includes the following steps,
A, natural strain obtains fermenting microbe after system ripe mashed potatoes mixed fermentation 48-72 hours；
B, adding clear water to dilute 15-30 times and be transplanted to the fermenting microbe in a includes in the tealeaves substrate of tealeaves, and stirring is equal
It is even to be put into togerther in fermentor, it is sealed, is kept for 28 degree to 32 degree of constant temperature, ferment 2.5-4 hours；The tealeaves is tea tree
Leaf.
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 15-30KHZ, ultrasonic wave
Extraction time is 20-60min, and extraction temperature is kept for 25-45 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24-36 hours, takes supernatant liquor, mistake
Filter supernatant liquor obtains clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds national standard preservative, obtains tealeaves antiseptic solution；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.Tealeaves of the present invention is using white
Tea effect is best, especially Anji County, Zhejiang Province white tea.If the tealeaves antiseptic solution is made using white tea, it is anti-that white tea can be referred to as
Rhzomorph solution.
Action principle: ultrasonic extraction, acting on medium using the cavitation effect of ultrasound ruptures plant cell wall in wink
Between complete, promote substance in plant cell release spread and dissolution, to significantly improve extraction efficiency.Tealeaves after enzymatic hydrolysis is aobvious
Work improves bioactivity, more conducively ultrasonic cavitation effect to the rapidly extracting of effective content of tea, due to fermentation enzymatic hydrolysis and
The cavitation effect of ultrasonic wave, which all acts in room temperature, to carry out so the activity of vegetable protein greatly improved, highly active vegetable albumen
Easy harmful mycoderm can be digested and be perforated, make the excessive and irreversible killing bacterium of sclerotium content, and albumen is sterilized
It is that high activity targets more, makes germ that can not generate drug resistance and lasting medicine.
Further, in step a, natural strain obtains fermenting microbe after system ripe mashed potatoes mixed fermentation 48 hours；Step b
In, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes to stir evenly and be put into togerther in the tealeaves substrate of tealeaves
It in fermentor, is sealed, is kept for 28 degree to 32 degree of constant temperature, ferment 3 hours；In step c, mixture obtained in b is carried out
Ultrasonic extraction, room temperature carry out, supersonic frequency 20-25KHZ, and the ultrasonic extraction time is 30-40min, and extraction temperature is kept
30-40℃；In step d, the substance extracted in c is separated by solid-liquid separation, settling tank is placed into and precipitates 24 hours, take upper layer clear
Liquid, filtering supernatant liquor obtain clear liquid.
Further, carbohydrase is added in step a.Promote catalysis, increases the controllability and stability of fermentation process.
Further, further include radix scutellariae and Fructus Forsythiae, the mass ratio in the tealeaves substrate is tealeaves in the tealeaves substrate:
Radix scutellariae: Fructus Forsythiae=2:1:1.
Further, the method for filtering described in step d is to be filtered using liquid chromatograph to the supernatant liquor in d,
The main peak value of liquid chromatograph is set as 23.868, obtains clear liquid.
Further, the method for filtering described in step d is to take the macromolecule that the supernatant liquor via hole diameter in d is 0.01 micron
Transparent clear liquid is obtained by filtration in film.
It realizes the purification to stoste, passes through beneficial mineral matter and microelement so that it is guaranteed that the various of stoste have
The reservation of beneficial ingredient, filtered off 99% or more all kinds of bacteriums and the colloid more much bigger than bacterium volume, iron rust, suspended matter,
Silt, larger molecular organics etc..
Further, the national standard preservative is any one in dehydroactic acid sodium, butyl p-hydroxybenzoate and calcium propionate
Kind.
Further, the natural strain is bacillus subtilis.
The present invention also proposes extracted broad-spectrum antibiotic in aforementioned manners, includes white tea, radix scutellariae, company in tealeaves substrate
It sticks up, water.The white tea that the white tea perhaps can newly adopt for old white tea generally can with the white tea tealeaves made or it is fresh from
The fresh white tealeaves without production under being picked on tea tree.
It further, further include international preservative in the tealeaves substrate, the mass ratio of tealeaves substrate is tealeaves: radix scutellariae: even
It sticks up: water: international preservative=4:2:2:92:0.3.International preservative can select dehydroactic acid sodium, butyl p-hydroxybenzoate
With any one in calcium propionate.
Compared with prior art, the invention has the following advantages: the present invention uses based on gradually Jiang'an Ji production white tea leaf
Raw material is wanted, broad-spectrum antibiotic is extracted through enzymatic hydrolysis ultrasonic wave mixing, has high activity broad-spectrum antibiotic to a variety of germs, fungi, disease
The microorganisms such as poison have stronger inhibition and killing effect, and the restraining and sterilizing bacteria effect of vegetable protein is that high activity targets more, makes
Drug resistance can not be generated to germ, ethanol refluxing process, decocting method and ion-exchange-resin process etc. in the prior art is solved and mentions
The problem of taking time long low efficiency, solves the problems such as bacterial drug resistance and reaches new antibiotic in multiple antibacterial fields
Level as hormone is without the effect of antibiotic and hormone side effect.
Specific embodiment
Below in conjunction with embodiment, the present invention is further illustrated.
Embodiment 1
A kind of method that ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, includes the following steps,
A, bacillus subtilis is made ripe mashed potatoes and mixes and obtain fermenting microbe after adding saccharification enzyme fermentation 48 hours；
B, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes to stir evenly in the tealeaves substrate of tealeaves
It is put into togerther in fermentor, is sealed, kept for 28 degree of constant temperature, ferment 3 hours；
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 20KHZ, ultrasonic extraction
Time is 30min, and extraction temperature is kept for 30 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24 hours, take supernatant liquor, utilizes
Liquid chromatograph is filtered supernatant liquor, and the main peak value of liquid chromatograph is set as 23.868, finally obtains clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds dehydroactic acid sodium preservative, obtains tealeaves antibiotic
Solution；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.
The broad-spectrum antibiotic extracted through the above method includes tealeaves, radix scutellariae, Fructus Forsythiae, water and dehydroactic acid in tealeaves substrate
Sodium preservative, mass ratio are 4:2:2:92:0.3.
Embodiment 2
A kind of method that ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, includes the following steps,
A, bacillus subtilis is made ripe mashed potatoes and mixes and obtain fermenting microbe after adding saccharification enzyme fermentation 48 hours；
B, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes to stir evenly in the tealeaves substrate of tealeaves
It is put into togerther in fermentor, is sealed, kept for 28 degree of constant temperature, ferment 3 hours；
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 20KHZ, ultrasonic extraction
Time is 30min, and extraction temperature is kept for 30 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24 hours, take supernatant liquor, utilizes
Aperture is that 0.01 micron of polymeric membrane (ultrafiltration membrane) is filtered supernatant liquor to obtain transparent clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds dehydroactic acid sodium preservative, obtains tealeaves antibiotic
Solution；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.
The broad-spectrum antibiotic extracted through the above method includes tealeaves, radix scutellariae, Fructus Forsythiae, water and dehydroactic acid in tealeaves substrate
Sodium preservative, mass ratio are 4:2:2:92:0.3.
Embodiment 3
A kind of method that ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, includes the following steps,
A, bacillus subtilis is made ripe mashed potatoes and mixes and obtain fermenting microbe after adding saccharification enzyme fermentation 48 hours；
B, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes to stir evenly in the tealeaves substrate of tealeaves
It is put into togerther in fermentor, is sealed, kept for 32 degree of constant temperature, ferment 3 hours；
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 25KHZ, ultrasonic extraction
Time is 40min, and extraction temperature is kept for 40 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24 hours, take supernatant liquor, utilizes
Aperture is that 0.01 micron of polymeric membrane is filtered supernatant liquor to obtain transparent clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds dehydroactic acid sodium preservative, obtains tealeaves antibiotic
Solution；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.
The broad-spectrum antibiotic extracted through the above method includes tealeaves, radix scutellariae, Fructus Forsythiae, water and dehydroactic acid in tealeaves substrate
Sodium preservative, mass ratio are 4:2:2:92:0.3.
Embodiment 4
A kind of method that ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, includes the following steps,
A, bacillus subtilis is made ripe mashed potatoes and mixes and obtain fermenting microbe after adding saccharification enzyme fermentation 48 hours；
B, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes to stir evenly in the tealeaves substrate of tealeaves
It is put into togerther in fermentor, is sealed, kept for 30 degree of constant temperature, ferment 3 hours；
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 22.5KHZ, ultrasonic wave extraction
Taking the time is 35min, and extraction temperature is kept for 35 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24 hours, take supernatant liquor, utilizes
Aperture is that 0.01 micron of polymeric membrane is filtered supernatant liquor to obtain transparent clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds dehydroactic acid sodium preservative, obtains tealeaves antibiotic
Solution；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.
The broad-spectrum antibiotic extracted through the above method includes tealeaves, radix scutellariae, Fructus Forsythiae, water and dehydroactic acid in tealeaves substrate
Sodium preservative, mass ratio are 4:2:2:92:0.3.
Embodiment 5
A kind of method that ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, includes the following steps,
A, bacillus subtilis is made ripe mashed potatoes and mixes and obtain fermenting microbe after adding saccharification enzyme fermentation 48 hours；
B, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes to stir evenly in the tealeaves substrate of tealeaves
It is put into togerther in fermentor, is sealed, kept for 31 degree of constant temperature, ferment 3 hours；
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 24KHZ, ultrasonic extraction
Time is 36min, and extraction temperature is kept for 36 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24 hours, take supernatant liquor, utilizes
Aperture is that 0.01 micron of polymeric membrane is filtered supernatant liquor to obtain transparent clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds dehydroactic acid sodium preservative, obtains tealeaves antibiotic
Solution；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.
The broad-spectrum antibiotic extracted through the above method includes tealeaves, radix scutellariae, Fructus Forsythiae, water and dehydroactic acid in tealeaves substrate
Sodium preservative, mass ratio are 4:2:2:92:0.3.
Embodiment 6
A kind of method that ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, includes the following steps,
A, bacillus subtilis is made ripe mashed potatoes and mixes and obtain fermenting microbe after adding saccharification enzyme fermentation 48 hours；
B, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes to stir evenly in the tealeaves substrate of tealeaves
It is put into togerther in fermentor, is sealed, kept for 29 degree of constant temperature, ferment 3 hours；
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 22KHZ, ultrasonic extraction
Time is 32min, and extraction temperature is kept for 32 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24 hours, take supernatant liquor, utilizes
Aperture is that 0.01 micron of polymeric membrane is filtered supernatant liquor to obtain transparent clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds dehydroactic acid sodium preservative, obtains tealeaves antibiotic
Solution；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.
The broad-spectrum antibiotic extracted through the above method includes tealeaves, radix scutellariae, Fructus Forsythiae, water and dehydroactic acid in tealeaves substrate
Sodium preservative, mass ratio are 4:2:2:92:0.3.
Embodiment 7
A kind of method that ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, includes the following steps,
A, bacillus subtilis is made ripe mashed potatoes and mixes and obtain fermenting microbe after adding saccharification enzyme fermentation 48 hours；
B, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes to stir evenly in the tealeaves substrate of tealeaves
It is put into togerther in fermentor, is sealed, kept for 28 degree of constant temperature, ferment 3 hours；
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 22KHZ, ultrasonic extraction
Time is 35min, and extraction temperature is kept for 35 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24 hours, take supernatant liquor, utilizes
Liquid chromatograph is filtered supernatant liquor, and the main peak value of liquid chromatograph is set as 23.868, finally obtains clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds butyl p-hydroxybenzoate preservative, obtains tealeaves
Antiseptic solution；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.
The broad-spectrum antibiotic extracted through the above method includes white tea, radix scutellariae, Fructus Forsythiae, water and para hydroxybenzene in tealeaves substrate
Butyl formate preservative, mass ratio are 4:2:2:92:0.3.
Embodiment 8
A kind of method that ultrasonic wave extraction enzymatic hydrolysis natural plants prepare broad-spectrum antibiotic, includes the following steps,
A, bacillus subtilis is made ripe mashed potatoes and mixes and obtain fermenting microbe after adding saccharification enzyme fermentation 48 hours；
B, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes to stir evenly in the tealeaves substrate of tealeaves
It is put into togerther in fermentor, is sealed, kept for 28 degree of constant temperature, ferment 3 hours；
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 22KHZ, ultrasonic extraction
Time is 35min, and extraction temperature is kept for 35 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24 hours, take supernatant liquor, utilizes
Liquid chromatograph is filtered supernatant liquor, and the main peak value of liquid chromatograph is set as 23.868, finally obtains clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds calcium propionate preservative, it is molten obtains tealeaves antibiotic
Liquid；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.
The broad-spectrum antibiotic extracted through the above method includes that white tea, radix scutellariae, Fructus Forsythiae, water and calcium propionate are anti-in tealeaves substrate
Rotten agent, mass ratio are 4:2:2:92:0.3.
Tealeaves involved in above-described embodiment refers to green tea, and the antibiotic effect made using white tea is best, especially
Anji County, Zhejiang Province white tea.
Antibacterium experiment, antimycotic experiment and anti-drug resistance experiment are made for the present invention, to prove wide spectrum of the invention
Antibiotic can efficiently kill bacterium, inhibit fungi growth and make germ that can not generate drug resistance.It takes in above-described embodiment 1 to 8
Tealeaves antiseptic solution carries out following test respectively.
The tealeaves antiseptic solution of Example 1 first is tested, and specific test data is as follows:
1, antibacterium is tested
Experimental strain sample is the bacillus by specific culture, and 3 hours are burnt in pressure cooker not extremely.
Sterilize sample: high concentration of chlorine dioxide solution, the ethacridine lactate solution of content 1%.Tealeaves antiseptic solution.
Experimental procedure
1) 10 milliliters of bacillus solution after cultivation is respectively added in A, B, C3 glasswares.
2) ClO 2 solution of 1 milliliter of content 01% is added in A bottles.
3) 1 milliliter of ethacridine lactate solution is added in B bottles.
4) 1 milliliter of white tea antibiotic liquid is added in C bottles.
5) A, B, C3 bottles are observed after half an hour under high magnification microscope in 28 DEG C of insulating boxs.
Testing result
Bacillus survival rate 100% in A bottles, ineffective sterilization.
Bacillus survival rate 100% in B bottles, ineffective sterilization.
Bacillus survival rate 20% in C bottles.
Conclusion, white tea antibiotic can efficiently kill the bacillus by culture, sterilizing efficiency much higher than chlorine dioxide and
Ethacridine lactate.
2, antimycotic experiment
The culture medium of inoculated fungi is added in two glasswares, one is put into 0.1 gram of compound ketoconazole ointment, separately
One is put into 0.1 gram of white tea antibiotic, is put into insulating box, and temperature is 28 DEG C, time 36h, checks that bacterial plaque inhibits area.
Experimental result: it is 1.5 centimetres of diameter that Ketoconazol/Clobetasol Propionate bacterial plaque, which inhibits area, without fungal infection in this area.It is white
It is 3 centimetres of diameter that tea antibiotic bacterial plaque, which inhibits area, without fungal infection in this area.
The inhibition fungi area of conclusion, white tea antibiotic is 4 times of compound ketoconazole ointment or so.
3, anti-drug resistance is tested
1) 5 milliliters of fluid nutrient medium of inoculated fungi are respectively added in bis- glasswares of A, B.
2) 1 milliliter of ethacridine lactate solution is added in A bottles, 1 milliliter of white tea antibiotic is added in B bottles, every mistake 24
Hour respectively adds 1 milliliter, until adding up to and respectively adding 5 milliliters for the 5th day.
3) 20% or so is respectively gone out after 24 hours, it is 40% or so, 5 that A bottles of sterilization rates, which are 25%, B bottles of sterilization rates, after 48 hours
It is observed under the microscope after it, A bottles of sterilization rates are that 30%, B bottles of sterilization rates are 80%.
Conclusion, when being added to 5 milliliters of dosage after ethacridine lactate solution 5 days, fungi obviously generates drug resistance, and white
The synergistic effect of tea antibiotic is obvious, does not generate drug resistance.
Specific embodiments of the present invention 2 to 8 also carry out above-mentioned test, and obtained test data is as shown in table 1 below:
Table 1
The present invention is not limited to above-mentioned specific embodiment, and persons skilled in the art are disclosed interior according to the present invention
Hold, can be implemented using other a variety of specific embodiments of the invention or all using design structure and think of of the invention
Road does simple change or change, both falls within protection scope of the present invention.
Claims (10)
1. a kind of method extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic, it is characterised in that: include the following steps,
A, natural strain obtains fermenting microbe after system ripe mashed potatoes mixed fermentation 48-72 hours；
B, adding clear water to dilute 15-30 times and be transplanted to the fermenting microbe in a includes to stir evenly one in the tealeaves substrate of tealeaves
It rises and is put into fermentor, be sealed, kept for 28 degree to 32 degree of constant temperature, ferment 2.5-4 hours；
C, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 15-30KHZ, ultrasonic extraction
Time is 20-60min, and extraction temperature is kept for 25-45 DEG C；
D, the substance extracted in c is separated by solid-liquid separation, places into settling tank and precipitate 24-36 hours, take supernatant liquor, in filtering
Layer clear liquid obtains clear liquid；
E, the processing of guaranteeing the quality that sterilizes is carried out to the clear liquid in d, adds national standard preservative, obtains tealeaves antiseptic solution；
F, in e tealeaves antiseptic solution freeze-drying after to get arrive broad-spectrum antibiotic.
2. a kind of method extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic according to claim 1, it is characterised in that:
In step a, natural strain obtains fermenting microbe after system ripe mashed potatoes mixed fermentation 48 hours；
In step b, adding clear water to dilute 20 times and be transplanted to the fermenting microbe in a includes in the tealeaves substrate of tealeaves, and stirring is equal
It is even to be put into togerther in fermentor, it is sealed, is kept for 28 degree to 32 degree of constant temperature, ferment 3 hours；
In step c, ultrasonic extraction is carried out to mixture obtained in b, room temperature carries out, supersonic frequency 20-25KHZ, ultrasound
Wave extraction time is 30-40min, and extraction temperature is kept for 30-40 DEG C；
In step d, the substance extracted in c is separated by solid-liquid separation, settling tank is placed into and precipitates 24 hours, take supernatant liquor, mistake
Filter supernatant liquor obtains clear liquid.
3. a kind of method extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic according to claim 1, it is characterised in that:
Carbohydrase is added in step a.
4. a kind of method extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic according to claim 1, it is characterised in that:
Further include radix scutellariae and Fructus Forsythiae, the mass ratio in the tealeaves substrate is tealeaves in the tealeaves substrate: radix scutellariae: Fructus Forsythiae=2:1:1,
The tealeaves is Anji County, Zhejiang Province white tea tealeaves.
5. a kind of method extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic according to claim 1, it is characterised in that:
The method of filtering described in step d is to be filtered using liquid chromatograph to the supernatant liquor in d, the main peak of liquid chromatograph
Value is set as 23.868, obtains clear liquid.
6. a kind of method extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic according to claim 1, it is characterised in that:
The method of filtering described in step d is that take the polymeric membrane that the supernatant liquor via hole diameter in d is 0.01 micron to be obtained by filtration transparent
Clear liquid.
7. a kind of method extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic according to claim 1, it is characterised in that:
The national standard preservative is any one in dehydroactic acid sodium, butyl p-hydroxybenzoate and calcium propionate.
8. a kind of method extracted enzymatic hydrolysis natural plants and prepare broad-spectrum antibiotic according to claim 1, it is characterised in that:
The nature strain is bacillus subtilis.
9. -8 any extracted broad-spectrum antibiotic of method according to claim 1, it is characterised in that: wrapped in tealeaves substrate
Include tealeaves, radix scutellariae, Fructus Forsythiae, water.
10. broad-spectrum antibiotic according to claim 9, it is characterised in that: the tealeaves is Anji County, Zhejiang Province white tea, the bottom
Further include international preservative in object, the mass ratio of tealeaves substrate is tealeaves: radix scutellariae: Fructus Forsythiae: water: international preservative=4:2:2:92:
0.3。
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