KR20130037956A

KR20130037956A - Method for preparing composition comprising fermented by using of dendropanax morbifera

Info

Publication number: KR20130037956A
Application number: KR1020110102543A
Authority: KR
Inventors: 최재홍
Original assignee: 최재홍
Priority date: 2011-10-07
Filing date: 2011-10-07
Publication date: 2013-04-17

Abstract

The present invention comprises a first step of washing and drying branches, leaves, and fruits as by-products of the yellow and yellow lacquer trees as main ingredients; A second step of cutting and crushing the dry matter that passed through the first step, and sterilizing various germs using an outoclave; A third step of mixing the crushed material having undergone the third step with fermentation microorganisms and distilled water in a culture tank to generate a mixture; A fourth step of producing a liquefied alkaline fermented product through bioreaction of the mixture passed through the third step on a bioreactor; A fifth step of sterilizing the fermentation microorganism by using the rapid sterilization apparatus (Outoclave) of the fermentation product passed through the fourth step; A sixth step of removing impurities by using a diatomaceous earth filter and then heating the fermented product after the fifth step and concentrating to have 25 to 60 brix; And a seventh step of drying the fermentation product that has passed through the sixth step to have a moisture content of 5 to 7% using a spray dryer.
Accordingly, according to the present invention, the acidity is obtained by obtaining an alkaline liquid fermentation product obtained by fermenting the branches, leaves, and fruits, which are by-products of yellow and yellow trees, through a bioreaction through fermentation microorganisms without adding a carbon source and a nitrogen source. PH of 8.96 (± 0.5) can be used to change alkaline materials suitable for natural dyeing, as well as various raw materials such as food, drinking, pharmaceutical, functional food, and fragrance raw materials. It is possible to mass-produce high value-added materials at low cost so that they can be easily absorbed.

Description

Method for preparing alkaline liquid fermented products using Hwangchil wood {Method for preparing composition comprising fermented by using of Dendropanax morbifera}

The present invention relates to a method for producing an alkaline liquid fermentation product using yellow lacquer, and more specifically, does not add a carbon source and a nitrogen source to branches, leaves, and fruits, which are by-products of the yellow lacquer tree and the yellow lacquer tree, unlike conventional methods. Fermented through biological reactions through fermentation microorganisms, and obtained fermented liquid fermentation products with alkaline pH. It relates to a method for producing an alkaline liquid fermentation product used.

Generally, the Hwangchil-tree is an evergreen tree of the dicotyledon-plant-mounted arboraceae, and the international scientific name is "Dendropanax morbifera", which means panacea, and is also called the golden tree. It is native to Korea and grows only 15m in height and blooms in the shape of inflorescence at the end of the branch in June, and the fruit ripens to black in October with elliptical nucleus of 7 ~ 19㎜ in length. And the effect of the yellow lacquer is Sesquiterpene, which contains water, gum, alcohol, esters, etc. The sap extracted from the bark of the yellow lacquer is a valuable substance due to its amazing pharmacological action and gentle odor. It is said to have been used only in the royal family.

Hwangchil-tree is recognized for its nutritional value, pharmaceutical value, and therapeutic value by modern scientific research results (Kyung Hee University, Pharmacology-Jeong, Se-Young, Kangwon University, Kwon, Myung-sang, etc.). In other words, according to papers and reports on the pharmacological effects of Hwangchil-tree, the efficacy includes a number of diseases such as blood circulation improvement (cholesterol, blood pressure), antioxidant (anti-aging), liver function improvement (hangover, fatigue recovery, detoxification), immunity enhancement and regeneration. It is reported to be effective.

Among the effects of Hwangchil-tree, studies on blood circulation improvement include `` Antiatherogenic activity of Dendropanax morbifera essential oil in rats ''. 2009 Aug.), the study on antioxidant activity, `` Study on the Antioxidant Functionality of Hwangchil Tree Extract '' (Chang, Gon, p13, p17), and the study on improving liver function and regeneration of hard tissues (bone and teeth), `` Korea Studies on the Biological Activity of Specialty Yellow Chilchi ”(Baik Woon Bong, 2003, p31, p37, p42 ~ 43),` `Isolation and anticomplement activity of compounds from Dendropanax morbifera '' In addition to BY, there is an overseas journal (J Ethnopharmacol. 2004 Feb) and the development of Hwangchil's stable production technology and the search for bioactive substances of Hwangchil tree origin (Jeonnam University Research Report (Ministry of Agriculture) p63, p82).

In addition, the documents on the neurostable include `` Development of Hwangchil Tree Resource Utilization Technology and Diversification of Utilization Method '' (Chonnam National University Research Report (published by the Ministry of Science and Technology): 1st year p110.). `` In vitro evaluation of the antiplasmodial activity of Dendropanax morbifera against chloroquine-sensitive strains of Plasmodium falciparum '' (Jil, Il-Min et al., 2009: Overseas Medical Journal Phytother Res. 2009 Apr 15) In the second year of the improvement and utilization of Hwangchil-tree resource utilization technology (Chonnam National University Research Report (Ministry of Science and Technology) p156), the article on the suppression of cancer cell proliferation, "Stable production technology development and A number of papers and research reports have been published, including the search for biologically active substances of origin "(Chonnam National University Research Report (published by the Ministry of Agriculture and Forestry) p63 ~ 65, p81). There is also a lot of modern medical pharmacological research.

As such, there is no place to throw away the entire tree, so it is a good plant of one stone two trillion, which has high economic value, greening of the natural environment, and raising economic income. The commercial value of Hwangchil tree is fresh dried, vinegar, cosmetics, soap, shampoo, seed oil, flavone oil and alcohol. It can be applied to the manufacture of tea leaves, the manufacture of tea products, high-grade food additives, mushroom culture trees, etc., and is still being studied in various directions and forms.

On the other hand, as a related art, Korean Patent Application Publication No. 2004-0107852, "Hwangchil extract manufacturing method having a skin whitening effect and according to the Hwangchil extract", Korean Patent Publication No. 10-0318019 "Hwangchil wood having anticancer activity Extract "," Oil Seed Extract of Hwangchil Tree with High Physiological Activity "of Korean Patent Publication No. 10-0663284," Hwangchil Tree Fermentation and Pharmaceutical Composition Comprising It "of Korean Patent Publication No. 10-0988072, Korean Unexamined Patent Publication The "preparation method of liquor using yellow chile tree" of No. 2003-0005098, the "ultraviolet-protective cosmetic composition using sap chil tree sap as an active ingredient" of Korean Laid-Open Patent Publication No. 2005-0036093 and the like are known.

However, the above-mentioned prior art document does not exist in the patent registration and publication of the alkaline liquid fermentation product and the microbial secondary metabolite composition through fermentation process using microorganisms on the leaves, branches, and berries of Hwangchil tree, In particular, the fruit of Hwangchil-tree has a lot of oil, so it is slow to be absorbed by the human body, it is difficult to use in the human body, and it is difficult to change the formulation in various forms such as powder and granule extract of Hwangchil-tree and by-products. It is difficult to mass-produce and process in large quantities, which shows its limitation in utilizing it.

Korean Laid-Open Patent Publication No. 2004-0107852 "Method of manufacturing yellowing extract having skin whitening effect and the resulting yellowing extract" Korean Patent Publication No. 10-0318019 "Hwangchil Tree Extract with Anticancer Activity" Korean Patent Publication No. 10-0663284 "Seed Extract of Hwangchil Tree with High Physiological Activity" Korean Patent Publication No. 10-0988072 "Hwangchil wood fermented product and pharmaceutical composition comprising the same" Korean Laid-Open Patent Publication No. 2003-0005098 "Method of manufacturing liquor using Hwangchil-tree" Korean Laid-Open Patent Publication No. 2005-0036093 "Sunscreen Cosmetic Composition with Sap of Sacred Tree Sap as Active Ingredient"

An object of the present invention for fundamentally improving the conventional problems as described above, to obtain an alkaline liquid fermented product fermented through the bioreaction through the fermentation microorganisms such as branches, leaves, fruits, etc., by-products of Hwangchil trees and Hwangchil trees Therefore, in the conventional fermentation process, fermentation proceeds smoothly when carbohydrate and nitrogen source are added. However, in the present invention, since the fermentation process is performed after excluding carbon source nitrogen source, the acidity is pH 8.96 (± 0.5). Alkaline substances can be obtained, so it can be changed into various raw materials such as dyeing raw materials suitable for natural dyeing, food raw materials, drinking raw materials, medical raw materials, functional food raw materials, and fragrance raw materials. Alkali liquid fermentation products using Hwangchil wood that can mass-produce large amounts of substances There haryeoneunde provided by law.

The present invention for achieving the above object, the first step of washing with the main raw materials, branches, leaves, berries of by-products of the Hwangchil and Hwangchil wood; A second step of cutting and crushing the dry matter that passed through the first step, and sterilizing various germs using an outoclave; A third step of mixing the crushed material having undergone the third step with fermentation microorganisms and distilled water in a culture tank to generate a mixture; A fourth step of producing a liquefied alkaline fermented product through bioreaction of the mixture passed through the third step on a bioreactor; A fifth step of sterilizing the fermentation microorganism by using the rapid sterilization apparatus (Outoclave) of the fermentation product passed through the fourth step; A sixth step of removing impurities by using a diatomaceous earth filter and then heating the fermented product after the fifth step and concentrating to have 25 to 60 brix; And a seventh step of drying the fermentation product that has passed through the sixth step to have a moisture content of 5 to 7% using a spray dryer.

In addition, the second step according to the invention is produced by a cutter or shredder 2 ~ 3㎜, using a sterilization device (Outoclave) characterized in that sterilizing various bacteria at a temperature of 80 ℃ ~ 132 ℃ for 10-30 minutes It is done.

In addition, the third step according to the present invention is a fermented microorganism cloud (Coriolus versicolor), mushroom cloud (Stereum hirustum), situation mushroom (Phellinus linteus), yeast fungus (Aspergillus oryzae), Bacillus (Bacillus) genus, horseshoe Mushrooms (Fomes fomentarius), Cordyceps sinensis (Coriolus sinensis), Lactobacillus (Lactobacillus) genus

~

After culturing to a number of individuals and mixing with distilled water, the mixture is characterized in that the mixture is mixed with distilled water in a 1: 1 ratio.

In addition, the fourth step according to the present invention is characterized in that the mixture is fermented by bioreaction for 2 to 60 days under a pressureless condition of the bioreactor with a temperature of 10 ~ 60 ℃, to produce a liquefied fermentation product exhibiting alkalinity .

In addition, the fifth step according to the present invention is characterized in that the fermentation products sterilize the fermentation microorganisms added during the bioreaction 20 to 30 minutes at a temperature of 80 ℃ ~ 132 ℃ using a rapid sterilization (Outoclave).

It should be understood, however, that the terminology or words of the present specification and claims should not be construed in an ordinary sense or in a dictionary, and that the inventors shall not be limited to the concept of a term It should be construed in accordance with the meaning and concept consistent with the technical idea of the present invention based on the principle that it can be properly defined. Therefore, the embodiments described in the present specification and the configurations shown in the drawings are merely the most preferred embodiments of the present invention, and not all of the technical ideas of the present invention are described. Therefore, It is to be understood that equivalents and modifications are possible.

As described in the above configuration and operation, according to the present invention, the branches, leaves, fruits and the like which are by-products of Hwangchil-tree and Hwangchil-tree are fermented through the bioreaction through the fermentation microorganism in the state in which the carbon source and the nitrogen source are not added to the bioprocess. By obtaining an alkaline liquid fermentation product, an alkaline substance with an acidity pH of 8.96 (± 0.5) can be obtained, which is suitable for natural dyeing, as well as food, drinking, pharmaceutical, functional food and cosmetic raw materials. It can be changed into various raw materials, and it is possible to mass-produce high value-added materials at low cost so that it can be easily absorbed into the body.

1 is an overall flow chart for producing a fermentation product using the hwangchil wood according to the present invention.

Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings.

The present invention relates to a method for producing a fermentation product using branches, leaves, and fruits which are by-products of Hwangchil trees and Hwangchil trees, and briefly described, rinsing, cutting / crushing, sterilization, microbial fermentation, bioreactors, Fermentation products are prepared through liquid fermentation, sterilization, filtration, concentration, and drying.

In the first step of the present invention, the main raw materials are washed with branches, leaves, and fruits which are by-products of Hwangchil and Hwangchil. The first step is to wash the main raw materials, and to wash the main raw materials of the branches, leaves, and fruits, which are by-products of Hwangchil trees and Hwangchil trees, and introduce an automatic device for quality stability and productivity improvement. Here, in the first step, the branches, leaves, and fruits, which are yellow chile and by-products, are washed and then provided without drying.

The second step of the present invention is to cut and crush the dried material that passed through the first step, and sterilize various germs using an autoclave (Outoclave). The second step is a step of cutting or shredding the main raw material that has undergone the washing step and then sterilizing. In particular, it is preferable to introduce a dedicated automated facility for improving productivity in cutting and shredding the main raw material to produce the shredded product.

At this time, the second step of the present invention is produced by a cutter or shredder 2 ~ 3㎜, using sterilization (Outoclave) sterilizes various bacteria at a temperature of 80 ℃ ~ 132 ℃ for 10-30 minutes. It produces washed main raw materials, namely, yellow chile and by-products (branches, leaves, fruits) to a size of 2-3 mm using a cutter or a crusher. Subsequently, in order to perform a smooth bioprocess by sterilizing the microorganisms contained in the lysate, sterilization is removed by sterilizing at a temperature of 80 ° C. to 132 ° C. for 10 to 30 minutes using an outoclave.

In the third step of the present invention, the lysate passed through the third step is mixed with fermentation microorganisms and distilled water in a culture tank to generate a mixture. The third step is to mix the fermentation microorganisms with the crushed main raw materials to produce a mixture, and to automate the process for quality stability and productivity improvement.

At this time, the third step of the present invention is a fermented microorganism cloud (Coriolus versicolor), mushroom cloud (Stereum hirustum), situation mushroom (Phellinus linteus), yeast fungus (Aspergillus oryzae), Bacillus (Bacillus) genus, horseshoe mushroom (Fomes fomentarius), Cordyceps sinensis (Coriolus sinensis), Lactobacillus (Lactobacillus) genus

~

After culturing to a number of individuals and mixing with distilled water, the mixture is mixed with distilled water in a 1: 1 ratio to produce a mixture. It is mixed with distilled water by using mushroom strains and lactic acid bacteria, which have proven their usefulness in fermentation microorganisms, to liquefy rapidly sterilized Hwangchil wood and its by-products such as leaves, branches, and fruits.

This results in the fermentation microorganisms of the above on a culture tank (a rapid incubator shaking incubator) in order to produce liquefied fermented powder.

~

After culturing in large quantities so that the number of individuals, and then mixed with distilled water, and then mix the crushed and distilled water in a 1: 1 ratio evenly to produce a mixture.

On the other hand, the secondary metabolite produced by basidiomycetes used as fermentation microorganisms has characteristics that are not found in other fungi. In other words, fermented microorganisms such as cloud mushroom (Coriolus versicolor), cloud mushroom (Stereum hirustum), situation mushroom (Phellinus linteus), yeast fungus (Aspergillus oryzae), horseshoe mushroom (Fomes fomentarius), and Cordyceps sinensis (Coriolus sinensis) Have The primary metabolites are essential for the survival of living organisms, but their physiological significance has been revealed, but the significance of secondary metabolites is still unclear. Antimicrobials, antimicrobials, enzymatic inhibitors, nucleic acid-related substances, amino acid-related substances, organic acids, phytohormones, and the like. Other physiologically active substances are peptides and protein-based physiologically active substances, which are still unknown, such as substances acting on insects, nematodes, germs, plant growth inhibitors, vitamin B1 breakdown substances and pharmacologically active substances .

Lactobacillus is a generic term for bacteria that break down sugars (lactic acid) to make lactic acid (lactic acid fermentation), and important bacteria that cause lactic acid fermentation are Streptococcus sp. And rod-shaped Lactobacillus genus. All are Gram-positive and painless anaerobic. In lactic acid fermentation, two molecules of lactic acid are produced from the hexose molecule, which may be accompanied by byproducts such as alcohol, acetic acid, succinic acid and carbon dioxide in addition to lactic acid. The former is called normal lactic acid fermentation or homo lactic acid fermentation, and the bacteria involved in this are called normal lactic acid bacteria. The latter is called heterolactic acid fermentation or heterolactic acid fermentation, and the bacteria involved in this are called heterolactic acid bacteria.

In the fourth step of the present invention, the mixture passed through the third step is subjected to bioreaction on a bioreactor to produce an alkaline liquefied fermentation product. The fourth step is the step of fermenting the mixture through the bioreaction with the fermentation microorganisms on a bioreactor to produce a liquefied fermentation product, which automates the process for quality stability.

Here, a suitable temperature range of the microbial bioreaction is about 10 ° C to 60 ° C degree, the bioreactor for liquefaction fermentation refers to a sterile continuous stirring bioreactor. In terms of the volume of bioreactor, 76% are designed to be non-agitated, non-sterilized, or aerobic. Bioreactors capable of maintaining sterility are not available at 10%. Aseptic bioreactors are used only for high value crops such as antibiotics. The bioreactor used in the present invention is to provide a convenient and inexpensive bioreaction operation using those that are designed to be agitated, sterilized, or aerobic because a rapid sterilization step follows the reaction.

At this time, the fourth step of the present invention fermented the mixture by bioreaction for 2 to 60 days under a pressureless condition of the bioreactor temperature of 10 ~ 60 ℃, to produce an alkaline liquefied fermentation product. The mixture is put into a bioreactor with a temperature of 10 ~ 60 ℃ automatically managed for 2 ~ 60 days, which are fermented microorganisms such as cloud mushroom (Coriolus versicolor), mushroom cloud (Stereum hirustum), situation mushroom (Phellinus linteus), and yeast fungus ( Aspergillus oryzae), Bacillus genus, Horseshoe mushroom (Fomes fomentarius), Cordyceps sinensis (Coriolus sinensis), and Lactobacillus genus Lactobacillus (Lactobacillus) genus to produce a liquefied fermentation product. The liquid fermented product has an acidity of alkaline substance of pH 8.96 (ㅁ 0.5). Here, the liquefaction (liquid) fermentation step is a process similar to the biodegradable food additives, mineral extracts in the field of material materials, which is a commonly used operation in bioprocess applications.

On the other hand, basidiomycetes, lactic acid bacteria to be used in the present invention is recognized as the most important microorganisms in the industry due to the diversity of chemical structure and species, especially in secondary metabolites. Metabolites are classified into primary metabolites and secondary metabolites. Primary metabolites are substances that play a fundamental role in living organisms such as processed amino acids, saccharides, lipids, and nucleic acids that are commonly found in plants, plants, and microorganisms , Secondary metabolites are various compounds such as alkaloids, terpenes, flavonoids and antibiotics, which are mainly found in plants and microorganisms, and they are becoming useful as medicines, fragrances, raw materials for flavor and functional foods and the like. Basidiomycetes and lactic acid bacteria continue to occupy an important position in the biomaterials industry through isolation of new strains and gene function.

The fifth step of the present invention sterilizes the fermentation microorganisms using the fast sterilization (Outoclave) of the fermentation product passed through the fourth step. Step 5 is sterilization of the liquefied fermentation product. It is recommended to introduce a dedicated automated facility for quality stability and productivity improvement.

At this time, the fifth step of the present invention sterilizes the fermentation microorganisms added during the bioreaction 20 to 30 minutes at a temperature of 80 ℃ ~ 132 ℃ using a fast sterilization (Outoclave) fermentation products. This is a fermentation microorganism that is a fermented microorganism that has been put into the bioreactor for 20-30 minutes at 80 ℃ ~ 132 ℃ temperature by using sterilization device (Outoclave), sterilized mushroom (Stereum hirustum). ), Phellinus linteus, yeast fungus (Aspergillus oryzae), Bacillus (Bacillus), horseshoe (Fomes fomentarius), Cordyceps (Coriolus sinensis), Lactobacillus (Lactobacillus) genus.

On the other hand, in the case of rapid sterilization, 10 minutes to 30 minutes at a temperature of 121 ° C., 10 minutes to 30 minutes are used at 132 ° C., but in the present invention, a bioreactor designed to be sterilized or aerobic is used, so the treatment temperature is higher. It is effective to treat for a long time 10-30 minutes at a temperature of 80 ℃ ~ 132 ℃.

In the sixth step of the present invention, the fermentation product passed through the fifth step is removed by using a diatomaceous earth filter, and then heated and concentrated to have 25 to 60 brix. The sixth step is a step of filtering and concentrating the liquefied fermentation product, and in particular, it is preferable to introduce a dedicated automated facility for improving productivity in filtering and concentrating the fermentation product. The fermentation product is first filtered through an diatomaceous earth filter, and then secondly concentrated to have a solid content of 25 to 60 Brix by evaporation.

That is, since the liquid fermentation product of which fermentation is completed is composed of many suspended substances and opaque liquid substance, impurities are almost filtered through a diatomaceous earth filter to maintain a state of transparent solid water-soluble substance. Subsequently, the liquid fermentation product is concentrated. Concentration is an operation of increasing the concentration of the solution by removing the solvent from the solution, and heating the solution to the boiling point (boiling point) by evaporation. The content of solids is concentrated to 25-60 Brix by removing water from the solution. This is because the liquid-reacted fermentation products are composed of many suspended substances and opaque liquid substances, so that the solids are substantially filtered to maintain the state of the transparent water-soluble substance.

In the seventh step of the present invention, the fermentation product passed through the sixth step is dried to have a moisture content of 5 to 7% using a spray dryer. The seventh step is a step of drying the concentrated fermentation product, it is preferable to introduce a dedicated automated equipment for improving productivity in drying the fermentation product. In order to diversify the application and to improve the storage capacity, it is necessary to increase the concentration of the soluble component by increasing the concentration of the soluble component by decreasing the cost of storing, packing and transporting the concentrated fermentation product, By weight.

That is, as the concentrated fermented product is dried with a spray dryer powder, it reduces the volume of storage, packaging, transportation, etc. while reducing the liquid volume to increase the concentration of soluble components, and increases the application range of cosmetic raw materials, food raw materials and pharmaceutical raw materials. It is possible to diversify the use and improve the shelf life to provide the optimum utility.

As described above, the liquid fermentation bioreaction step includes fermentation, in the use of fermentation products prepared by using the yellow lacquer tree and by-products, the fermentation process including the liquid reaction fermentation step is repeated on the yellow chile tree. It is easy to absorb into skin pores when used as a cosmetic raw material by breaking the chain of molecules of the active substance contained in small units, and it is suitable for natural dyeing with alkaline substance with pH 8.96 (± 0.5). When used, it is easy to absorb in the intestine, so that the active ingredient can provide more effective absorption to the human body, and in addition, it can be used in various ways for food ingredients, medical ingredients and functional food raw materials.

As described above, the method for preparing the fermentation product of the present invention sterilizes the bacteria of Hwangchil-tree for liquid fermentation of Hwangchil-tree branches, leaves, and berries, and thus, by adding fermentation microorganisms in a bioreactor, The cell membranes of branches, leaves, and fruits are destroyed, and the active ingredients containing microorganisms are broken down into water solubility. The dissolved fermented products are suitable as natural dyeing ingredients with alkaline pH 8.96 (± 0.5), cosmetic ingredients, It provides a composition that can be easily absorbed in the body when used as a food raw material, a quasi-drug raw material. In addition, it can provide a functional material of the secondary metabolite between the hwangchil and the microorganism according to the fermentation to the microorganism.

That is, in the present invention, the acidity of the hot water extract by distilled water as a method of producing the liquid fermentation product through the bioprocess through the fermentation microorganisms in the bioreactor using the fermented microorganisms such as the Hwangchil-tree branches, leaves, and fruits. 0.5), which is close to acidic, solid fermentation was added to nitrogen and carbon source, and after bioprocessing, the acidity of the extract was neutral at pH 6.47 (± 0.5). The fermentation products through bioprocessing by adding only specific fermentation microorganisms without adding nitrogen source and carbon source were studied as a dyeing material suitable for natural dyeing because acidity can obtain alkaline substance at pH 8.96 (± 0.5). In addition, the liquid fermentation product, which is alkaline due to liquid fermentation, can be used for fragrance raw materials, food raw materials and pharmaceutical raw materials.

It will be apparent to those skilled in the art that various modifications and variations can be made in the present invention without departing from the spirit and scope of the invention as defined by the appended claims. It is therefore intended that such variations and modifications fall within the scope of the appended claims.

Claims

A first step of washing the main material of branches, leaves, and fruits which are by-products of Hwangchil trees and Hwangchil trees;
A second step of cutting and crushing the raw material that has passed through the first step, and sterilizing various germs using an outoclave;
A third step of mixing the crushed material having undergone the third step with fermentation microorganisms and distilled water in a culture tank to generate a mixture;
A fourth step of producing a liquefied alkaline fermented product through bioreaction of the mixture passed through the third step on a bioreactor;
A fifth step of sterilizing the fermentation microorganism by using the rapid sterilization apparatus (Outoclave) of the fermentation product passed through the fourth step;
A sixth step of removing impurities by using a diatomaceous earth filter and then heating the fermented product after the fifth step and concentrating to have 25 to 60 brix; And
Preparing an alkaline liquid fermentation product using the yellow lacquer tree, characterized in that it comprises a; seventh step of drying the fermentation product passed through the sixth step to have a moisture content of 5 ~ 7% by using a spray dryer (Spray dryer) How to.

The method of claim 1,
The second step is to produce a cutting machine or shredder 2 ~ 3㎜, using a sterilization device (Outoclave) using sterilized bacteria to sterilize various bacteria at a temperature of 80 ℃ ~ 132 ℃ for 10-30 minutes Method for producing an alkaline liquid fermentation product.

The method of claim 1,
The third step is fermented microorganisms (Coriolus versicolor), mushrooms (Stereum hirustum), mushrooms (Phellinus linteus), yeast fungus (Aspergillus oryzae), Bacillus (Bacillus) genus, horseshoe (Fomes fomentarius), Cordyceps sinensis (Coriolus sinensis), Lactobacillus (Lactobacillus) genus

~

The method for producing an alkaline liquid fermentation product using sulfur lacquer, characterized by culturing to a number of individuals and mixing with distilled water, and then mixing the crushed water in a 1: 1 ratio.

The method of claim 1,
In the fourth step, the mixture is fermented by a bioreactor for 2 to 60 days under a pressureless condition of the bioreactor at a temperature of 10 to 60 ° C., and an alkaline liquid phase using sulfuric acid wood is used to produce a liquefied fermentation product having alkalinity. Process for preparing fermentation product.

The method of claim 1,
The fifth step is an alkaline liquid fermented product using a yellow lacquer tree, characterized in that the fermentation products sterilize the microorganisms added during the bioreaction for 20 to 30 minutes at a temperature of 80 ℃ ~ 132 ℃ using a rapid sterilization device (Outoclave) How to manufacture.