CN104755493B - 用于在转基因动物中生产人类抗体的复杂的染色体工程 - Google Patents

用于在转基因动物中生产人类抗体的复杂的染色体工程 Download PDF

Info

Publication number
CN104755493B
CN104755493B CN201380041315.4A CN201380041315A CN104755493B CN 104755493 B CN104755493 B CN 104755493B CN 201380041315 A CN201380041315 A CN 201380041315A CN 104755493 B CN104755493 B CN 104755493B
Authority
CN
China
Prior art keywords
human
gene
bovine
vector
heavy chain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201380041315.4A
Other languages
English (en)
Chinese (zh)
Other versions
CN104755493A (zh
Inventor
Y·黑岩
H·松下
A·佐野
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SAB LLC
Original Assignee
SAB LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SAB LLC filed Critical SAB LLC
Publication of CN104755493A publication Critical patent/CN104755493A/zh
Application granted granted Critical
Publication of CN104755493B publication Critical patent/CN104755493B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Knock-in vertebrates, e.g. humanised vertebrates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
    • C07K16/462—Igs containing a variable region (Fv) from one specie and a constant region (Fc) from another
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/101—Bovine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/20—Pseudochromosomes, minichrosomosomes
    • C12N2800/208—Pseudochromosomes, minichrosomosomes of mammalian origin, e.g. minichromosome

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Animal Husbandry (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201380041315.4A 2012-08-03 2013-08-05 用于在转基因动物中生产人类抗体的复杂的染色体工程 Active CN104755493B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261679288P 2012-08-03 2012-08-03
US61/679,288 2012-08-03
PCT/US2013/053618 WO2014022853A1 (en) 2012-08-03 2013-08-05 Complex chromosome engineering for production of human antibodies in transgenic animals

Publications (2)

Publication Number Publication Date
CN104755493A CN104755493A (zh) 2015-07-01
CN104755493B true CN104755493B (zh) 2020-12-25

Family

ID=48949296

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380041315.4A Active CN104755493B (zh) 2012-08-03 2013-08-05 用于在转基因动物中生产人类抗体的复杂的染色体工程

Country Status (9)

Country Link
US (1) US9902970B2 (enExample)
EP (1) EP2880055B1 (enExample)
JP (1) JP6426606B2 (enExample)
KR (1) KR102159163B1 (enExample)
CN (1) CN104755493B (enExample)
AU (1) AU2013296182B2 (enExample)
IN (1) IN2015DN01700A (enExample)
NZ (1) NZ703973A (enExample)
WO (1) WO2014022853A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104904661B (zh) * 2015-06-05 2018-07-24 杭州正因生物技术有限公司 一种人源化小鼠
US11072649B2 (en) 2015-11-25 2021-07-27 Sab, Llc Systems and methods for the production of human polyclonal antibodies
EP3533867A4 (en) * 2016-10-31 2020-07-22 National University Corporation Tottori University NON-HUMAN ANIMAL PRODUCING HUMAN ANTIBODIES AND PROCESS FOR THE PREPARATION OF HUMAN ANTIBODIES USING IT
WO2019015677A1 (en) * 2017-07-21 2019-01-24 Beijing Biocytogen Co., Ltd GENETICALLY MODIFIED IMMUNOGLOBULIN HEAVY CHAIN ANIMALS
CN108486125B (zh) * 2018-03-27 2024-01-05 重庆金迈博生物科技有限公司 一种核酸分子及其在制备人源单域抗体中的应用
CN108486126A (zh) * 2018-03-27 2018-09-04 重庆金迈博生物科技有限公司 一种核酸分子及其在人源化抗体中的应用
US12331320B2 (en) 2018-10-10 2025-06-17 The Research Foundation For The State University Of New York Genome edited cancer cell vaccines
CN110128524B (zh) * 2019-06-04 2021-05-07 中国农业科学院兰州兽医研究所 猪IgA+ B细胞类别转换标志物序列及检测方法
KR20220140800A (ko) * 2020-02-12 2022-10-18 에스에이비, 엘엘씨 항-흉선세포 글로불린
EP4204005A1 (en) 2020-08-31 2023-07-05 Sab, Llc Ungulate-derived polyclonal immunoglobulin specific for coronavirus protein and uses thereof
US20240010734A1 (en) * 2020-11-13 2024-01-11 Sab, Llc Ungulate-derived polyclonal immunoglobulin specific for egfr and uses thereof
EP4244247A4 (en) * 2020-11-13 2024-12-18 Sab, Llc HUPTA-DERIVED POLYCLONAL IMMUNOGLOBULIN SPECIFIC FOR PD-L1 AND USES THEREOF
US20250320279A1 (en) * 2021-11-30 2025-10-16 Sab, Llc Ungulate-derived polyclonal immunoglobulin specific for influenza virus and uses thereof
WO2024173854A1 (en) 2023-02-17 2024-08-22 Sab, Llc Ungulate-derived polyclonal immunoglobulin specific for influenza virus and uses thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5625126A (en) * 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
CN1668187A (zh) * 2002-05-17 2005-09-14 赫马技术有限公司 能够产生人抗体的转基因有蹄动物
CA2780945A1 (en) * 2009-11-17 2011-05-26 Kyowa Hakko Kirin Co., Ltd. Human artificial chromosome vector

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6833268B1 (en) 1999-06-10 2004-12-21 Abgenix, Inc. Transgenic animals for producing specific isotypes of human antibodies via non-cognate switch regions
US7414170B2 (en) 1999-11-19 2008-08-19 Kirin Beer Kabushiki Kaisha Transgenic bovines capable of human antibody production
AU2001297523B2 (en) 2000-11-17 2007-01-18 Sab, Llc Expression of xenogenous (human) immunoglobulins in cloned, transgenic ungulates
WO2002092812A1 (fr) * 2001-05-11 2002-11-21 Kirin Beer Kabushiki Kaisha CHROMOSOME HUMAIN ARTIFICIEL CONTENANT LE GENE A CHAINE LEGERE DE L'ANTICORPS HUMAIN $g(l)
CN1852925A (zh) * 2003-07-15 2006-10-25 人类多克隆治疗公司 人源化免疫球蛋白基因座
CN102164676B (zh) * 2008-07-10 2014-10-29 菲活机器制造公司 带有分离器的珠磨机
LT2346994T (lt) * 2008-09-30 2022-03-10 Ablexis, Llc Knock-in pelė, skirta chimerinių antikūnų gamybai
EP2564695B1 (en) 2009-07-08 2015-04-15 Kymab Limited Animal models and therapeutic molecules
WO2012157589A1 (ja) * 2011-05-13 2012-11-22 国立大学法人宮崎大学 細胞接着阻害剤、細胞増殖阻害剤、並びに癌の検査方法および検査用キット

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5625126A (en) * 1990-08-29 1997-04-29 Genpharm International, Inc. Transgenic non-human animals for producing heterologous antibodies
CN1668187A (zh) * 2002-05-17 2005-09-14 赫马技术有限公司 能够产生人抗体的转基因有蹄动物
CA2780945A1 (en) * 2009-11-17 2011-05-26 Kyowa Hakko Kirin Co., Ltd. Human artificial chromosome vector

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Characterization of the human Ig guest locus in HAC transgenic cattle;Anae Duteau;《Doctoral Dissertations of University of Massachusette Amherst》;20050101 *
Fully human antigen-specific polyclonal antibody responses induced in cloned human artificial chromosome transchromosomic cattle;Yoon Jong Choi;《Doctoral Dissertations of University of Massachusette Amherst》;20050101 *

Also Published As

Publication number Publication date
AU2013296182B2 (en) 2018-03-08
JP6426606B2 (ja) 2018-11-21
HK1210621A1 (en) 2016-04-29
IN2015DN01700A (enExample) 2015-05-22
NZ703973A (en) 2018-04-27
EP2880055A1 (en) 2015-06-10
JP2016504012A (ja) 2016-02-12
AU2013296182A1 (en) 2015-02-19
CN104755493A (zh) 2015-07-01
WO2014022853A1 (en) 2014-02-06
KR102159163B1 (ko) 2020-09-23
EP2880055B1 (en) 2017-06-28
US20150211020A1 (en) 2015-07-30
US9902970B2 (en) 2018-02-27
KR20150039820A (ko) 2015-04-13

Similar Documents

Publication Publication Date Title
CN104755493B (zh) 用于在转基因动物中生产人类抗体的复杂的染色体工程
CN102741404B (zh) 人类人工染色体载体
RU2559524C2 (ru) Продуцирующие антитела млекопитающие, не являющиеся человеком
EP3251503B1 (en) Genetic engineering of mice for the production of chimeric antibodies
JP2003501103A (ja) 非同種スイッチ領域を介して、ヒト抗体の特定のアイソタイプを産生するためのトランスジェニック動物
HK1210621B (en) Complex chromosome engineering for production of human antibodies in transgenic animals
HK1176638A (en) Human artificial chromosome vector
HK1176637A (en) Human artificial chromosome vector
HK1176637B (en) Human artificial chromosome vector

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
EXSB Decision made by sipo to initiate substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant