CN104738102A - Application of actinidia chinensis root-bark extract for preparing virucide for plants - Google Patents
Application of actinidia chinensis root-bark extract for preparing virucide for plants Download PDFInfo
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Abstract
The invention discloses application of an actinidia chinensis root-bark extract for preparing a virucide for plants. Actinidia chinensis root-bark is aired, crushed and then subjected to a certain processing process to prepare actinidia chinensis root-bark antiviral soluble liquor, water aqua and micro-emulsions which can be used for crops such as tobaccos, peppers, tomatoes, summer squashes, potatoes and the like for preventing and treating various viroses of plants such as tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), potato virus X (PVX), potato virus Y (PVY) and the like. The virucide for plants disclosed by the invention is not only good in antiviral effect, but also is safe to the environment, human, livestock, natural enemies of pests and other beneficial organisms. The virucide for plants is simple in preparation process, low in cost and suitable for being popularized and applied.
Description
Technical field
The invention belongs to plant and application thereof, be specifically related to the application of Chinese gooseberry root bark extract for Antiphytoviral.
Background technology
Plant virus a kind ofly infects active cytozoicus pathogen by having of forming of nucleic acid and protein, and according to the 8th report of ICTV (ICTV), the plant virus kind that the current whole world finds reaches 1122.Plant disease caused by plant virus have the title of " plant cancer ", and it is only second to the mycosis original to the extent of injury of crops, and almost often kind of crops are all subject to 2 ~ 3 kinds of viral harm.The loss that the whole world is caused by plant virus every year about 400 hundred million dollars, only the harm that causes of tobacco mosaic virus (Tobacco mosaic virus, TMV) is just more than 100,000,000 dollars.
Because plant virus has strict parasitism, it colonizes in plant cell or even cell nucleus the synthesis with protein coat that copies carrying out nucleic acid (RNA or DNA), forms new virion.This difficulty of prevention and cure just determining the viroses of plant is large, and general medicament does not have effect.20 beginning of the century Allard report the inhibiting substances that there is TMV in Phytolacca acinosa juice, and the emphasis preventing and treating plant virus afterwards turns to natural plant extract direction gradually.Particularly from since the nineties in last century, people have found the inhibiting substances of many Antiphytovirals successively from natural products, provide the foundation for developing efficient, single-minded, safe plant virus natural inhibitor.
Chinese gooseberry Actinidia chinensis Planch. is Actinidiaceae actinidia, and its root, also known as Chinese actinidia root, is Chinese unique wheat, is mainly distributed in the province such as Shaanxi, Jiangxi, Hunan, Hubei, Fujian, Zhejiang, Henan, Anhui of China.Chinese gooseberry complete stool is all pharmaceutically acceptable, the traditional Chinese medical science thinks its root and root skin is cold in nature, bitter and puckery flavor, there is effect of activating blood circulation and reducing swelling, expelling wind and removing dampness, reducing fever and causing diuresis, heat radiation hemostasis, can be used for the illnesss such as treatment hepatitis, rheumatic arthritis, dysentery, filariosis, cancer of the stomach, breast cancer.Modern age pharmaceutical research find Chinese gooseberry root have scavenging capacity oxygen radical, antiviral, protect the liver, inhibition tumor cell growth, improve the functions such as immunity.
The Chinese gooseberry root chemical composition now reported is flavones, triterpene, steroidal, anthraquinone, phenolic acid, carbohydrate and other polytype compounds mainly, wherein based on triterpenes components.The pharmacologically active of the reactive compound found is mainly manifested in medicinal activity aspect, as effect of antitumor, enhanced machine body immunity function, protection liver, anti-oxidant, scavenging free radicals, anti-rotavirus.Up to the present, about Chinese gooseberry root bark extract and metabolite thereof, report is had no to the effect of disease, worm, crop smothering in agricultural production.
Summary of the invention
To the object of the invention is to the root bark extract of Chinese gooseberry (Actinidia chinensis Planch.), for raw material, work out the plant resource anti-plant virus agent that Antiphytoviral effect is high, nuisanceless, cheap.
For achieving the above object, the technical scheme that the present invention takes is:
Chinese gooseberry root bark extract is for the preparation of the application of anti-plant virus agent.
Concrete, described Chinese gooseberry root bark extract is the material obtained after Chinese gooseberry root skin crushed material is carried out alcohol extracting.
More specifically, the particle diameter of described Chinese gooseberry root skin crushed material is 1 ~ 5mm, and the solvent that alcohol extracting uses is ethanol, and alcohol extracting temperature is 50 ~ 70 DEG C.
Further, the solvent that alcohol extracting uses is mass fraction 95% ethanol, and the alcohol extracting time is 18 ~ 20 hours.
Meanwhile, described plant virus comprises tobacco mosaic virus, cucumber mosaic virus and Potyvirus.
A kind of anti-plant virus agent, the formulation of this anti-plant virus agent is microemulsion, and described microemulsion is prepared by following raw materials by weight:
Chinese gooseberry root bark extract: 15% ~ 30%, organic solvent: 0 ~ 55%, surfactant: 5% ~ 20%, antifreezing agent: 5% ~ 10%, surplus is water, and the percentage by weight sum of raw material is 100%.
Or the formulation of this anti-plant virus agent is soluble liquid, and described soluble liquid is prepared by following raw materials by weight:
Chinese gooseberry root bark extract 15% ~ 30%, organic solvent: 0% ~ 55%, surfactant: 5% ~ 30%, surplus is ethanol, and the percentage by weight sum of raw material is 100%.
Or the formulation of this anti-plant virus agent is aqua, and described aqua is prepared by following raw materials by weight:
Chinese gooseberry root bark extract: 10% ~ 20%, organic solvent: 0% ~ 40%, surfactant: 5% ~ 30%, antifreezing agent: 5% ~ 10%, surplus is water, and the percentage by weight sum of raw material is 100%.
Chinese actinidia root skin anti-plant virus agent made by the present invention has the following advantages:
(1) by carrying out the Kiwi extract of different cultivars studying, the present invention finds that the root bark extract of Chinese gooseberry is remarkable to the preventive effect of plant virus, and is obviously better than other kind Kiwi extracts to the preventive effect of plant virus;
(2) in addition, inventor has also carried out the research of anti-phytoviral activity to the extract of the different parts of Chinese kiwi fruit plant, find to only have the extract of the root skin zone position of Chinese gooseberry remarkable to the preventive effect of plant virus, other positions even do not have anti-phytoviral activity;
(3) the anti-phytoviral activity wide spectrum of Chinese gooseberry extract of the present invention, can be used for tobacco, capsicum, tomato, custard squash, potatoes and other crops being prevented and treated tobacco mosaic virus disease (Tobaccomosaic virus, TMV), Cucumber Mosaic Virus (Cucumber mosaic virus, and potato virus disease (Potato Virus X, PVX CMV); Potato Virus Y, PVY) etc. various plants virus disease.
Embodiment
Plant resource anti-plant virus agent of the present invention, with perennial Chinese gooseberry root bark extract for active substance, add certain proportion auxiliary agent and carry out formulation, environment-friendly type preparation microemulsion, aqua or soluble liquid can be obtained, Chinese gooseberry root bark extract of the present invention is the material after alcohol extracting, optional alcohol extraction solvent is ethanol, has no adverse effects to the antiviral effect of this plant extracts.
Plant virus of the present invention comprises: tobacco mosaic virus disease (Tobacco mosaicvirus, TMV), Cucumber Mosaic Virus (Cucumber mosaic virus, and potato virus disease (Potato Virus X, PVX CMV); Potato Virus Y, PVY).
The extracting method of Chinese gooseberry root bark extract of the present invention comprises:
A. meal Chinese gooseberry root skin plant tissue cracker being crushed to 1 ~ 5mm minces;
B. minced by the meal of steps A gained and use 95% alcohol steep, Extracting temperature is 50 ~ 70 DEG C, and extraction time is 18 ~ 20 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate.
Organic solvent of the present invention is one or more the mixture in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone and dimethyl formamide;
Described emulsifier is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, this Pan's series, tween and agricultural anion-phosphate;
Described surfactant is one or more the mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, the addition product of phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane and aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Described antifreezing agent is ethylene glycol or glycerine.
In order to particularity and the further active component exploring its activity of clear and definite Chinese gooseberry root skin antiviral activity, inventor has done the work of several lower three aspects in advance.
One, Chinese gooseberry root skin belongs to the difference of other plant antiviral activity together:
Inventor compares the antiviral activity of the root bark extract of Chinese gooseberry and other several actinidias nearer with its affiliation, and result shows, and the antiviral activity of Chinese gooseberry root bark extract is significantly better than other kinds.The results are shown in Table 1.
Several Chinese actinidia root bark extract of table 1 is to TMV inactivation in vitro
Note: 1, reagent agent is 20% root bark extract microemulsion;
2, in table, data are the mean value of 3 repetitions; In " preventive effect " hurdle, refer in the level of α=0.05 in significant difference with letter different after ordered series of numbers mark.
Two, the determination at Chinese gooseberry antiviral activity position:
Inventor determines the external inactivate activity of Chinese gooseberry different parts extract to TMV, result shows, the antiviral activity of different parts extract is widely different, under same concentrations, root bark extract has significant activity, root, stem, leaf all do not show activity, and complete stool shows certain activity, may also be the effects (table 2) due to antiviral activity in root skin.Accordingly, inventor infers, in Chinese gooseberry, antiviral activity concentrates on root skin zone position, and therefore, determination Chinese gooseberry root bark extract of the present invention is the primary raw material of antiviral activity.
Table 2 Chinese gooseberry different parts extract is to TMV inactivation in vitro
Note: 1, "-" expression does not show inactivate activity.
2, in table, data are the mean value of 3 repetitions; In " preventive effect " hurdle, refer in the level of α=0.05 in significant difference with letter different after ordered series of numbers mark.
Three, the broad spectrum activity of Chinese gooseberry root bark extract antiviral activity:
Inventor determines the external inactivate activity of Chinese gooseberry root bark extract to several frequently seen plants virus disease, result shows, it all shows certain activity to TMV, CMV, PVX and PVY, and this illustrates, the anti-phytoviral activity composition containing broad spectrum activity in Chinese gooseberry root skin.
Table 3 Chinese gooseberry root bark extract is to the inactivation in vitro of several plant virus disease
Note: in table, data are the mean value of 3 repetitions; In " preventive effect " hurdle, refer in significant difference in the level of α=0.05 with letter different after ordered series of numbers mark, as follows.
On the basis of above test, in order to develop the antiviral activity preparation of Chinese gooseberry root bark extract further, it is made microemulsion, soluble liquid and aqua respectively.Test through inventor proves, 20% Chinese actinidia root skin microemulsion, 20% Chinese actinidia root skin soluble liquid and 10% Chinese actinidia root severe edema due to hypofunction of the spleen agent have good control efficiency to tobacco mosaic virus (TMV), wherein optimum with the control efficiency of 20% Chinese actinidia root skin microemulsion.In order to better understand essence of an invention, describe the technology contents of invention below in detail by embodiment, but invention is not limited to these embodiments.
Four, example prepared by concrete preparation:
The preparation of embodiment 1:20% Chinese actinidia root skin microemulsion
Take Chinese actinidia root skin concentrate 20kg, be dissolved in 30kg ethyl acetate, then add 15kg calcium dodecyl benzene sulfonate, 5kg styrene acid APEO, 5kg ethylene glycol, mixes under high velocity agitation, is heated to about 40 DEG C; Drip deionized water 25kg under high-speed stirred, control the rate of addition of water, make temperature remain on about 40 DEG C; Water dropwises, and is warming up to 50 DEG C, stirs 1 hour, obtained 20% Chinese actinidia root skin microemulsion 100kg.The stability, outward appearance etc. of preparation meet the requirement of commercial preparation.
The preparation of embodiment 2:20% Chinese actinidia root skin soluble liquid
Take Chinese actinidia root skin concentrate 20kg, be dissolved in 30kg ethyl acetate, add 5kg alkylphenol polyoxyethylene again, 1kg APG A, ethanol polishing is to 100kg, after mixing, stir 10 ~ 30 minutes with mixing speed 800 ~ 1000 revs/min, Chinese actinidia root skin soluble liquid 100kg can be obtained.The stability, outward appearance etc. of preparation meet the requirement of commercial preparation.
The preparation of embodiment 3:10% Chinese actinidia root severe edema due to hypofunction of the spleen agent
Take Chinese actinidia root skin concentrate 10kg, be dissolved in 30kg ethyl acetate, add 5kg alkylphenol polyoxyethylene again, 1kg APG A, 5kg ethylene glycol, water polishing to 100kg, after mixing, stir 10 ~ 30 minutes with mixing speed 800 ~ 1000 revs/min, Chinese actinidia root skin soluble liquid 100kg can be obtained.The stability, outward appearance etc. of preparation meet the requirement of commercial preparation.
Five, 3 kinds of Chinese actinidia root skin antivirotics are to the indoor bioassay of TMV:
(1) to the external passivation effect of TMV
Adopt the withered spot method of half leaf to measure, choosing vigorous, that growing way is consistent 5 ~ 6 leaf phase Nicotiana glutinosas (Nicotiana glutinosa) of growth is withered spot host.Take vein as boundary, Zuo Banye inoculates liquid and viral equal-volume mixed liquor, and right half leaf inoculation distilled water and viral equal-volume mixed liquor compare, and virus inoculation concentration is 10 μ g/mL, and passivation time is 5min, rinses inoculation blade surface immediately after inoculation with clear water.Often process inoculation 4 leaves, test repetition 3 times, adds up withered spot number after 3d, calculates inhibiting rate.
Inhibiting rate (%)=(contrast withered spot number-process withered spot number)/contrast withered spot number × 100
Measurement result is in table 4.
Table 43 kinds of Chinese actinidia root skin antivirotics are to TMV inactivation in vitro
(2) to the inhibition of TMV First aggression
Adopt the withered spot method of half leaf, 5 ~ 6 leaf phase Nicotiana glutinosas choosing growing way consistent, healthy are withered spot host, virus inoculation after smearing liquid 6h, 12h, 24h, blank is clear water process, and 4 leaves are inoculated in each process, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 5.
Table 53 kinds of Chinese actinidia root skin antivirotics are to the inhibition of TMV First aggression
(3) to the inhibition of TMV propagation
Adopt the withered spot method of half leaf, 5 ~ 6 leaf phase Nicotiana glutinosas choosing growing way consistent, healthy are withered spot host, after virus inoculation 6h, 12h, 24h, smear liquid, blank is clear water process, and 4 leaves are inoculated in each process, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 6.
The inhibition that table 63 kinds of Chinese actinidia root skin antivirotics are bred TMV
From table 4,5,6,3 kinds of Chinese actinidia root skin antivirotics are to the passivation of TMV, and First aggression, proliferation inhibiting effect are good, and wherein, the effect of 20% Chinese actinidia root skin microemulsion is better than other two kinds of formulations, apparently higher than contrast medicament moroxydine. second copper.
(4) to the pot experiment of TMV
1) to the protective effect of common cigarette
Select the 5-6 leaf phase, common cigarette (Nicotiana tabacum) the confession examination that growing way is consistent, healthy and strong.Prevention group (dispenser 3 times then inoculate TMV) and control group (only inoculating not dispenser) 2 groups are established in test.With the 200 times of liquid sprayings of 3 kinds of Chinese actinidia root skin antivirotics, contrast medicament moroxydine. second copper 500 times of common cigarettes of liquid spraying process, every 5d dispenser once, is total to dispenser three times.Inoculate TMV after last dispenser 24h, inoculum density is 1:20 (W/V).After inoculation, 14d, 21d check incidence, statistics disease index and control efficiency.Often process 60 strain cigarette seedlings, test repetition 3 times.Calculate preventive effect.
The incidence of disease=(diseased plant number of getting a haircut everywhere/process total strain number) × 100%
Disease index=∑ (sick progression × diseased plant number)/(the highest sick level × total strain number of each process) × 100
Disease index growth rate=(spray medicine after disease refer to-spray medicine before disease refer to)/spray medicine before disease refer to × 100%
Control efficiency=(contrast disease refers to-process that disease refers to)/contrast disease to refer to × 100%
Protective effect measurement result is as shown in table 7.
Table 73 kinds of Chinese actinidia root skin antivirotics are to the protective effect measurement result of common cigarette
2) to the therapeutic action of common cigarette
Select the 5-6 leaf phase, common cigarette (Nicotiana tabacum) the confession examination that growing way is consistent, healthy and strong.Treatment group (after inoculation TMV dispenser 3 times) and control group (only inoculating not dispenser) 2 groups are established in test.Inoculate malicious source TMV, inoculum density is 1:20 (W/V), and after inoculation, every 5d dispenser once, dispenser three times altogether.Medicament is the 200 times of liquid sprayings of 3 kinds of Chinese actinidia root skin antivirotics, contrast medicament moroxydine. the 500 times of liquid sprayings of second copper.Incidence is checked, statistics disease index and control efficiency during 14d, 21d respectively at last dispenser.Often process 60 strain cigarette seedlings, test repetition 3 times.Calculate preventive effect.Therapeutic action measurement result is as shown in table 8.
Table 83 kinds of Chinese actinidia root skin antivirotics are to the therapeutic action measurement result of common cigarette
Results from pot experiment test shows (table 4, table 5); 3 kinds of Chinese actinidia root skin antivirotics show good prevention and therapy effect to tobacco mosaic virus disease; its protection and result for the treatment of are all better than contrasting medicament; wherein, 20% Chinese actinidia root skin microemulsion protective effect relative control effect is higher than contrast medicament about 25%.
Six, 3 kinds of Chinese actinidia root skin antivirotics are to the drug effect test of field zone of TMV:
Small plot experiment is randomized arrangement, and repeat 3 times, plot area is depending on actual conditions, but area can not be less than 30 ㎡, experimental field select to require fertility evenly, crop-planting is consistent with managerial skills, state of an illness generation and the extent of injury more even; Protection row will be established between each process and around test region.Blade face conventional spray is carried out, with the moroxydine of 20% with 3 kinds of Chinese actinidia root skin antivirotics, 100,200,400 times of liquid. second copper wetting powder 500 times of liquid carry out blade face conventional spray for contrasting medicament, and establish clear water to contrast; All reagent agents must carry out secondary dilution.The idiopathy initial stage starts to spray medicine, after spray 1 time every 7d, totally 3 times.10d Investigate incidence before first time spray medicine and after last spray medicine and statistics disease index, calculate preventive effect, the results are shown in Table 9.
Table 93 kinds of Chinese actinidia root skin antivirotic control tobacco virus drug effect test of field zone
As can be known from the above table, 3 kinds of Chinese actinidia root skin antivirotics have good control efficiency to tobacco mosaic virus, obviously be better than medicament when dilution 100,200,400 times and contrast viral A, preventive effect significant difference, and the average preventive effect of 20% Chinese actinidia root skin microemulsion is better than the average preventive effect of 20% Chinese actinidia root skin soluble liquid and 10% Chinese actinidia root severe edema due to hypofunction of the spleen agent, as seen Chinese actinidia root bark extract is prepared into microemulsion advantageously in the control to plant virus.
Claims (8)
1. Chinese gooseberry root bark extract is for the preparation of the application of anti-plant virus agent.
2. apply as claimed in claim 1, it is characterized in that, described Chinese gooseberry root bark extract is the material obtained after Chinese gooseberry root skin crushed material is carried out alcohol extracting.
3. apply as claimed in claim 2, it is characterized in that, the particle diameter of described Chinese gooseberry root skin crushed material is 1 ~ 5mm, and the solvent that alcohol extracting uses is ethanol, and alcohol extracting temperature is 50 ~ 70 DEG C.
4. apply as claimed in claim 3, it is characterized in that, the solvent that alcohol extracting uses is mass fraction 95% ethanol, and the alcohol extracting time is 18 ~ 20 hours.
5. the application as described in claim 1,2,3 or 4, is characterized in that, described plant virus comprises tobacco mosaic virus, cucumber mosaic virus and Potyvirus.
6. an anti-plant virus agent, is characterized in that, the formulation of this anti-plant virus agent is microemulsion, and described microemulsion is prepared by following raw materials by weight:
Chinese gooseberry root bark extract: 15% ~ 30%, organic solvent: 0 ~ 55%, surfactant: 5% ~ 20%, antifreezing agent: 5% ~ 10%, surplus is water, and the percentage by weight sum of raw material is 100%.
7. anti-plant virus agent as claimed in claim 6, it is characterized in that, the formulation of this anti-plant virus agent is soluble liquid, and described soluble liquid is prepared by following raw materials by weight:
Chinese gooseberry root bark extract 15% ~ 30%, organic solvent: 0% ~ 55%, surfactant: 5% ~ 30%, surplus is ethanol, and the percentage by weight sum of raw material is 100%.
8. anti-plant virus agent as claimed in claim 6, it is characterized in that, the formulation of this anti-plant virus agent is aqua, and described aqua is prepared by following raw materials by weight:
Chinese gooseberry root bark extract: 10% ~ 20%, organic solvent: 0% ~ 40%, surfactant: 5% ~ 30%, antifreezing agent: 5% ~ 10%, surplus is water, and the percentage by weight sum of raw material is 100%.
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| CN105145675A (en) * | 2015-09-02 | 2015-12-16 | 孙凯 | Poultry raising house air disinfectant and preparation method thereof |
| CN105660734A (en) * | 2016-04-25 | 2016-06-15 | 青岛奕奕和农牧科技有限公司 | Chinese herbal medicine disinfectant for breeding industry |
| CN108892668A (en) * | 2018-07-19 | 2018-11-27 | 贵州大学 | Benzsulfamide chalcones derivative, the preparation method and the usage of purine-containing ring |
| CN109169716A (en) * | 2018-07-27 | 2019-01-11 | 普定绿盛园生态农业有限公司 | A kind of composition and preparation method thereof for preventing and treating grey mould fruit rot of strawberry |
| CN111657305A (en) * | 2020-05-06 | 2020-09-15 | 西北农林科技大学 | Application of fennel extract in preparation of anti-plant virus agent and anti-plant virus agent |
| CN112369437A (en) * | 2020-10-23 | 2021-02-19 | 江西省林业科学院 | Method and medicament for treating citrus huanglongbing |
| CN115251053A (en) * | 2022-07-19 | 2022-11-01 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Sclareol plant antiviral preparation and application thereof |
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| CN105145675A (en) * | 2015-09-02 | 2015-12-16 | 孙凯 | Poultry raising house air disinfectant and preparation method thereof |
| CN105660734A (en) * | 2016-04-25 | 2016-06-15 | 青岛奕奕和农牧科技有限公司 | Chinese herbal medicine disinfectant for breeding industry |
| CN108892668A (en) * | 2018-07-19 | 2018-11-27 | 贵州大学 | Benzsulfamide chalcones derivative, the preparation method and the usage of purine-containing ring |
| CN109169716A (en) * | 2018-07-27 | 2019-01-11 | 普定绿盛园生态农业有限公司 | A kind of composition and preparation method thereof for preventing and treating grey mould fruit rot of strawberry |
| CN111657305A (en) * | 2020-05-06 | 2020-09-15 | 西北农林科技大学 | Application of fennel extract in preparation of anti-plant virus agent and anti-plant virus agent |
| CN111657305B (en) * | 2020-05-06 | 2021-09-07 | 西北农林科技大学 | Application of fennel extract in preparation of anti-plant virus agent and anti-plant virus agent |
| CN112369437A (en) * | 2020-10-23 | 2021-02-19 | 江西省林业科学院 | Method and medicament for treating citrus huanglongbing |
| CN112369437B (en) * | 2020-10-23 | 2021-11-23 | 江西省林业科学院 | Method and medicament for treating citrus huanglongbing |
| CN115251053A (en) * | 2022-07-19 | 2022-11-01 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Sclareol plant antiviral preparation and application thereof |
| CN115251053B (en) * | 2022-07-19 | 2023-09-26 | 中国农业科学院烟草研究所(中国烟草总公司青州烟草研究所) | Sclareol plant antiviral preparation and application thereof |
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