CN103141525A - Rhodiola eurycarpa anti-plant virus agent and preparation method thereof - Google Patents
Rhodiola eurycarpa anti-plant virus agent and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a botanical antiviral agent with a Rhodiola eurycarpa extract as an effective component and its preparation method. The preparation can be in the form of a soluble liquid agent, a water agent and a microemulsion. The preparation contains 10%-40% of the Rhodiola eurycarpa extract and the balance assistants. The anti-plant virus agent can be used for preventing and treating a cucumber mosaic virus (CMV) disease on tobaccos, chillies, tomatoes, cucumbers and other crops. In addition to good antiviral effects, the anti-plant virus agent is also safe to the environment, human, livestock, natural enemies of pests and other beneficial organisms. The anti-plant virus agent has a simple preparation process and a low cost, thus being suitable for popularization and application.
Description
Technical field
The invention belongs to botanical pesticide and application thereof, be specifically related to a kind of wide arnotto red-spotted stonecrop anti-plant viral disease preparation and preparation method thereof.
Background technology
Plant virus is a kind ofly to infect active cytozoicus pathogen by having of forming of nucleic acid and protein, and according to the 8th report of ICTV (ICTV), the plant virus kind found of the whole world reaches 1122 at present.The caused plant disease of plant virus have the title of " plant cancer ", and its extent of injury to crops is only second to the mycosis original, and almost every kind of crops all are subject to the harm of 2～3 kinds of viruses.Approximately 400 hundred million dollars of the losses that is caused by plant virus in whole world every year, cucumber mosaic virus (Cucumber mosaic virus, CMV) be host range at most, one of plant virus of the widest, tool Economic Importance distributes.
Because plant virus has obligate parasitism to plant cell, the needed matter and energy of virus replication place is provided by host plant fully, causes virus and host's metabolism to combine together; Plant lacks complete immune system in addition, in a single day virus infects plant, just can survive indefinite duration in plant cell, until the host is dead.Therefore, the medicine of control of plant virosis only is difficult to optionally challenge virus and does not injure host cell, causes efficient, single-minded, the safe anti-virus formulation research of screening at present to face great difficulty.
Be cross protection except taking traditional Anti-virus Disease Breeding, attenuated strain at present, improve the methods such as culture technique, insecticide control biography virus mediator and transgenosis, the scientific research personnel has also worked out the chemical prevention and control method of some viral diseases of plants.But up to the present, only have the sub-fraction anti-plant virus agent to obtain practical application, these preparations are greatly mainly with putting prevention first, and how undesirable drug effect is.Because people study early antivirotic in medicine or veterinary drug, some anti-plant virus agents (as agricultural virazole) are exactly to develop from anti-human or zoosis toxic agent.Therefore, further investigated Antiphytoviral natural materials is conducive to research, exploitation and the application of anti-plant virus agent.
Wide arnotto red-spotted stonecrop (Rhodiola eurycarpa) belongs to the Crassulaceae Rhodida plant.Perennial herb.Main root is long, diameter 3 ?4 millimeters.Collar elongation, diameter 5 ?6 millimeters, old stem is not remaining or minority is remaining, the sterile plate minority.Scape is most, high 20 ?25 centimetres, top is by the micro emulsion caput.The leaf wire is fallen lanceolar, long 1.3 ?2.5 centimetres, wide 3 ?4 millimeters, the anxious point of tip, base portion is gradually narrow, the edge have thin sawtooth 1 ?6.Give birth on inflorescence top, intensive, have 5 ?20 flowers, diameter 2.5 ?3 centimetres; Dioecism; Sepal 5, narrow linear, long 2 ?3.5 millimeters, wide 0.4 ?0.6 millimeter; Petal 5, aubergine or faint yellow, wire fall lanceolar, long 4 ?5 millimeters, wide 1 ?1.5 millimeters, blunt; In male flower, stamen 10, reach 6 millimeters; Scale 5, square, long 1 millimeter, wide 0.8 millimeter, tip has little lacking; Female flower carpel 5, tiltedly avette, long 6 millimeters, upright, base portion is gradually narrow, outside of belly protuberance.The Follicle radish somewhat from, handle is closely arranged, short beak is arranged, upright; Seed is avette, and there is wing at two ends, 2 millimeters of total lengths.6 ?July of florescence, 8 ?September of fruit phase.
At present, there is not yet the report of wide arnotto red-spotted stonecrop Antiphytoviral aspect.
Summary of the invention
The object of the invention is to, take wide arnotto red-spotted stonecrop (Rhodiola eurycarpa) as raw material, provide high, nuisanceless, cheap plant resource anti-plant virus agent of a kind of Antiphytoviral effect and preparation method thereof.
In order to realize above-mentioned task, the present invention takes following technical solution:
A kind of wide arnotto red-spotted stonecrop anti-plant virus agent is characterized in that, this anti-plant virus agent that makes is take wide arnotto red-spotted stonecrop extract as principal component and add auxiliary agent to process, and the preparation form is soluble liquid, aqua and microemulsion; Wherein:
Described microemulsion is prepared by following raw materials by weight:
Wide arnotto red-spotted stonecrop extract: 20%～40%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
Described soluble liquid is prepared by following raw materials by weight:
Wide arnotto red-spotted stonecrop extract: 20%～40%, organic solvent: 30%～60%, surfactant: 5%～30%, the percentage by weight sum of raw material is 100%;
Described aqua is prepared by following raw materials by weight:
Wide arnotto red-spotted stonecrop extract 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
2, wide arnotto red-spotted stonecrop anti-plant virus agent as claimed in claim 1 is characterized in that:
Described organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, the mixture of one or more in dimethyl formamide;
The mixture of one or more that described emulsifier is alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural the moon in the sub-?phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
Above-mentioned organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, the mixture of one or more in dimethyl formamide;
The mixture of one or more that described emulsifier is alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, this Pan's series, tween, agricultural the moon in the sub-?phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
The preparation method of above-mentioned wide arnotto red-spotted stonecrop anti-plant virus agent is characterized in that:
The preparation method of described microemulsion is:
A, wide arnotto red-spotted stonecrop complete stool is minced with the meal that the plant tissue cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt the solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate.
C, with the concentrate of step B gained, be placed according to formula ratio and organic solvent, surfactant, antifreezing agent and water the still that is mixed, the still temperature that is mixed be 35 ?60 ℃, mixing time is 30 minutes, namely gets wide arnotto red-spotted stonecrop Antiphytoviral microemulsion after stirring.
The preparation method of described soluble liquid is:
A, wide arnotto red-spotted stonecrop complete stool is minced with the meal that cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt the solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate.
C, with the concentrate of step B gained, be placed according to formula ratio and organic solvent and surfactant the still that is mixed, the still temperature that is mixed be 35 ?60 ℃, mixing time is 30 minutes) namely get wide arnotto red-spotted stonecrop Antiphytoviral soluble liquid after stirring.
The preparation method of described aqua is:
A, wide arnotto red-spotted stonecrop complete stool is minced with the meal that cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt the solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate.
C, with the concentrate of step B gained, be placed according to formula ratio and organic solvent, surfactant, antifreezing agent and water the still that is mixed, the still temperature that is mixed be 35 ?60 ℃, mixing time is 30 minutes, namely gets wide arnotto red-spotted stonecrop Antiphytoviral aqua after stirring.
The made wide arnotto red-spotted stonecrop anti-plant virus agent of the present invention has the following advantages:
1. anti-phytoviral activity is remarkable, can be used for preventing and treating Cucumber Mosaic Virus (Cucumber mosaic virus, CMV) on the crops such as tobacco, capsicum, tomato, cucumber;
2. to people, animal, pest natural enemy and other beneficial organism safety, environment compatibility is good;
3. biogenic pesticide, use rear noresidue to poison;
4. simple, with low cost, suitable the promoting the use of of aboundresources, preparation method.
Embodiment
In order better to understand essence of an invention, the below describes the technology contents of invention in detail with embodiment, but invention is not limited to these embodiment.
Wide arnotto red-spotted stonecrop anti-plant virus agent of the present invention take wide arnotto red-spotted stonecrop extract as active substance, adds the certain proportion auxiliary agent to carry out formulation, can make environment-friendly type preparation microemulsion, aqua or soluble liquid.
Microemulsion is prepared by following raw materials by weight:
Wide arnotto red-spotted stonecrop extract: 20%～40%, solvent 30%～60%, surfactant 5%～30%, antifreezing agent 5%～10%, surplus is water, the summation of raw material is 100%.
Soluble liquid is prepared by following raw materials by weight:
Wide arnotto red-spotted stonecrop extract: 20%～40%, organic solvent: 30%～60%, surfactant: 5%～30%, the percentage by weight sum of raw material is 100%;
Aqua is prepared by following raw materials by weight:
Wide arnotto red-spotted stonecrop extract: 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%.
The preparation method of above-mentioned wide arnotto red-spotted stonecrop anti-plant virus agent, adopt following steps:
1, the acquisition of wide arnotto red-spotted stonecrop extract:
A, wide arnotto red-spotted stonecrop complete stool is minced with the meal that the plant tissue cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt the solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract to being equivalent to 1kg dry powder/kg concentrate, is wide arnotto red-spotted stonecrop extract through Vacuum Concentration;
2, wide arnotto red-spotted stonecrop anti-plant virus agent preparation
1) with the concentrate of step B gained, according to formula ratio and organic solvent, surfactant, antifreezing agent and water by being placed in the still that is mixed, the still temperature that is mixed is 35 ℃～60 ℃, and mixing time is 30 minutes, namely gets wide arnotto red-spotted stonecrop Antiphytoviral microemulsion after stirring;
2) with the concentrate of step B gained, be placed according to formula ratio and organic solvent and surfactant the still that is mixed, the still temperature that is mixed be 35 ?60 ℃, mixing time is 30 minutes, namely gets wide arnotto red-spotted stonecrop Antiphytoviral soluble liquid after stirring.
3) with the concentrate of step B gained, be placed according to formula ratio and organic solvent, surfactant, antifreezing agent and water the still that is mixed, the still temperature that is mixed is 35 ℃～60 ℃, and mixing time is 30 minutes, namely gets wide arnotto red-spotted stonecrop Antiphytoviral aqua after stirring.
Through inventor's evidence, 30% wide arnotto red-spotted stonecrop microemulsion, 30% wide arnotto red-spotted stonecrop soluble liquid and 20% wide arnotto red-spotted stonecrop aqua have good control efficiency to cucumber mosaic virus (CMV).
Below the embodiment that the inventor provides.
The preparation of the wide arnotto red-spotted stonecrop of embodiment 1:30% microemulsion
Take wide arnotto red-spotted stonecrop concentrate 30kg, be dissolved in 45kg ethyl acetate, then add the 15kg calcium dodecyl benzene sulfonate, the 5kg fatty alcohol-polyoxyethylene ether, ethylene glycol 5kg, mixed under high-speed stirred, be heated to 40 ℃ of left and right; Drip deionized water 30kg under high-speed stirred, control the rate of addition of water, make temperature remain on 40 ℃ of left and right; Water dropwises, and is warming up to 50 ℃, stirs 1 hour, makes 20% wide arnotto red-spotted stonecrop microemulsion 100kg.The stability of preparation, outward appearance etc. meet the requirement of commodity preparation.
The preparation of the wide arnotto red-spotted stonecrop of embodiment 2:30% soluble liquid
Take wide arnotto red-spotted stonecrop concentrate 30kg, be dissolved in 45kg ethyl acetate, add again the 5kg polyoxyethylene nonylphenol ether, 1kg APG A, the ethanol polishing is to 100Kg, after mixing, stirred 10～30 minutes with 80～100 rev/mins of mixing speeds, can make wide arnotto red-spotted stonecrop soluble liquid 100kg.The stability of preparation, outward appearance etc. meet the requirement of commodity preparation.
The preparation of the wide arnotto red-spotted stonecrop of embodiment 3:20% aqua
Take wide arnotto red-spotted stonecrop concentrate 20kg, be dissolved in 30kg ethyl acetate, add again the 5kg castor oil polyoxyethylene ether, 1kg APG A, 5kg ethylene glycol, the water polishing is to 100Kg, after mixing, stirred 10～30 minutes with 80～100 rev/mins of mixing speeds, can make wide arnotto red-spotted stonecrop aqua 100kg.The stability of preparation, outward appearance etc. meet the requirement of commodity preparation.
The indoor bioassay of the wide arnotto red-spotted stonecrop of embodiment 4:3 kind antivirotic to CMV
(1) to the external passivation effect of CMV
Adopt the half withered spot method of leaf to measure, choose that growth is vigorous, growing way is consistent 5～6 the leaf phase Chenopodium amaranticolor be withered spot host.Take vein as the boundary, Zuo Banye inoculation liquid and viral equal-volume mixed liquor, right half leaf inoculation distilled water and viral equal-volume mixed liquor compare, and virus inoculation concentration is 10 μ g/mL, and passivation time is 5min, rinses the inoculation blade surface with clear water immediately after inoculation.4 leaves of every processing inoculation, test repeats 3 times, adds up withered spot number after 3d, calculates inhibiting rate.
Inhibiting rate (%)=(contrast Ku Ban Shuo ?process withered spot number)/contrast withered spot number * 100%
Measurement result sees Table 1.
The wide arnotto red-spotted stonecrop of table 1:3 kind antivirotic is to the CMV inactivation in vitro
| Reagent agent | Passivation time/(min) | Extension rate/doubly | Inhibiting rate/% |
| 30% wide arnotto red-spotted stonecrop microemulsion | 5 | 200 | 82.07a |
| 30% wide arnotto red-spotted stonecrop soluble liquid | 5 | 200 | 79.24b |
| 20% wide arnotto red-spotted stonecrop aqua | 5 | 200 | 72.53c |
| 20% moroxydine. second copper wetting powder | 5 | 200 | 37.67d |
Annotate: in table, data are the mean value of 3 repetitions; In " preventive effect " hurdle, refer to be remarkable difference on α=0.05 level with different letters after the ordered series of numbers mark.Following table is same.
(2) inhibition that CMV is just infected
Adopt the half withered spot method of leaf, choose growing way consistent, healthy 5～6 the leaf phase Chenopodium amaranticolor be withered spot host, virus inoculation after smearing liquid 6h, 12h, 24h, blank is that clear water is processed, and each processes 4 leaves of inoculation, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 2.
The inhibition that the wide arnotto red-spotted stonecrop of table 2:3 kind antivirotic just infects CMV
The inhibition of (3) CMV being bred
Adopt the half withered spot method of leaf, choose growing way consistent, healthy 5～6 the leaf phase Chenopodium amaranticolor be withered spot host, smear liquid after virus inoculation 6h, 12h, 24h, blank is that clear water is processed, and each processes 4 leaves of inoculation, repeats 3 times, add up withered spot number after 3d, calculate inhibiting rate.The results are shown in Table 3.
The inhibition of the wide arnotto red-spotted stonecrop of table 3:3 kind antivirotic to CMV propagation
By table 1,2,3 as can be known, the passivation to CMV of 3 kinds wide arnotto red-spotted stonecrop antivirotics is just infected, inhibited proliferation is good, apparently higher than contrast medicament moroxydine. second copper.
The pot experiment of the wide arnotto red-spotted stonecrop of embodiment 4:3 kind antivirotic to CMV
(1) to the protective effect of cucumber virus disease
Select 5 ?6 leaf phases, consistent, the healthy and strong cucumber (Cucumis sativus Linn.) of growing way is for examination.2 groups of prevention group (dispenser 3 times then inoculate CMV) and control groups (only inoculating not dispenser) are established in test.With 200 times of liquid sprayings of 3 kinds wide arnotto red-spotted stonecrop antivirotics, contrast medicament moroxydine. cucumber seedling is processed in 500 times of liquid sprayings of second copper, and every 5d dispenser once is total to dispenser three times.Inoculate CMV after last dispenser 24h, inoculum density is 1:20(W/V).After inoculation, 14d, 21d check incidence, statistics disease index and control efficiency.Every processing 60 strain cucumber seedlings, test repeats 3 times.Calculate preventive effect.
The incidence of disease=(the total strain number of diseased plant number/processing of getting a haircut everywhere) * 100%
Disease index=∑ (sick progression * diseased plant number)/(the highest sick level * respectively process total strain number) * 100
Before disease index growth rate=(after the spray medicine, disease refers to-spray that disease refers to before medicine)/spray medicine disease refer to * 100%
Control efficiency=(the contrast disease refers to-process that disease refers to)/contrast disease to refer to * 100%
The protective effect measurement result is as shown in table 4.
The protective effect measurement result of the wide arnotto red-spotted stonecrop of table 4:3 kind antivirotic to the cucumber virus disease
(2) to the therapeutic action of cucumber virus disease
Select 5 ?6 leaf phases, consistent, the healthy and strong cucumber (Cucumis sativus Linn.) of growing way is for examination.2 groups for the treatment of group (inoculation CMV after dispenser 3 times) and control groups (only inoculating not dispenser) are established in test.Inoculate malicious source CMV, inoculum density is 1:20(W/V), after inoculation, every 5d dispenser once, is total to dispenser three times.Medicament is 200 times of liquid sprayings of 3 kinds wide arnotto red-spotted stonecrop antivirotics, contrast medicament moroxydine. 500 times of liquid sprayings of second copper.Check incidence during respectively at 14d, the 21d of last dispenser, statistics disease index and control efficiency.Every processing 60 strain cucumber seedlings, test repeats 3 times.Calculate preventive effect.The therapeutic action measurement result is as shown in table 5.
The therapeutic action measurement result of the wide arnotto red-spotted stonecrop of table 5:3 kind antivirotic to the cucumber virus disease
Results from pot experiment test shows (table 4, table 5), and 3 kinds wide arnotto red-spotted stonecrop antivirotics show good prevention and result for the treatment of to Cucumber Mosaic Virus, and its prevention and result for the treatment of all are better than the contrast medicament, and relative control effect is higher than contrast medicament 10% left and right.
The drug effect test of field zone of the wide arnotto red-spotted stonecrop of embodiment 5:3 kind antivirotic to CMV
The residential quarter crop is cucumber, and small plot experiment is randomized arrangement, repeat 3 times, residential quarter area depending on actual conditions, but area is not less than 30 ㎡, experimental field select to require fertility evenly, crop-planting is consistent with managerial skills, the state of an illness occur and the extent of injury more even; To establish guard row between each is processed and around the test region.Carrying out the blade face conventional spray, the moroxydine take 20% with 100,200,400 times of liquid of 3 kinds wide arnotto red-spotted stonecrop antivirotics. 500 times of liquid of second copper wetting powder carry out the blade face conventional spray as contrasting medicament, and establish the clear water contrast; All reagent agents must be carried out secondary dilution.The idiopathy initial stage begins to spray medicine, afterwards every 7d spray 1 time, totally 3 times.Spray for the first time before medicine and the 10d Investigate incidence after last spray medicine and statistics disease index, calculate preventive effect, the results are shown in Table 6.
The wide arnotto red-spotted stonecrop of table 6:3 kind antivirotic control cucumber virus disease drug effect test of field zone
As can be known from the above table, 3 kinds wide arnotto red-spotted stonecrop antivirotics have good control efficiency to cucumber mosaic virus, obviously are better than medicament contrast 20% moroxydine in the situation that dilute 100,200 times. second copper wetting powder, preventive effect significant difference.
Claims (4)
1. one kind wide arnotto red-spotted stonecrop anti-plant virus agent, is characterized in that, this anti-plant virus agent that makes is take wide arnotto red-spotted stonecrop extract as principal component and add auxiliary agent to process, and the preparation form is soluble liquid, aqua and microemulsion; Wherein:
Described microemulsion is prepared by following raw materials by weight:
Wide arnotto red-spotted stonecrop extract: 20%～40%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%;
Described soluble liquid is prepared by following raw materials by weight:
Wide arnotto red-spotted stonecrop extract: 20%～40%, organic solvent: 30%～60%, surfactant: 5%～30%, the percentage by weight sum of raw material is 100%;
Described aqua is prepared by following raw materials by weight:
Wide arnotto red-spotted stonecrop extract 10%～30%, organic solvent: 30%～60%, surfactant: 5%～30%, antifreezing agent: 5%～10%, surplus is water, the percentage by weight sum of raw material is 100%.
2. wide arnotto red-spotted stonecrop anti-plant virus agent as claimed in claim 1 is characterized in that:
Described organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, the mixture of one or more in dimethyl formamide;
The mixture of one or more that described emulsifier is alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural the moon in the sub-?phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
3. the preparation method of wide arnotto red-spotted stonecrop anti-plant virus agent claimed in claim 1, is characterized in that, adopts following steps:
A, wide arnotto red-spotted stonecrop complete stool is minced with the meal that the plant tissue cracker is crushed to 1～5mm.
B, the meal of steps A gained is minced adopt the solvent method lixiviate, extraction solvent is any one or a few in ethanol, ethyl acetate, chloroform, benzinum, water, extracting temperature is 50～80 ℃, extraction time is 6～12 hours, extract through Vacuum Concentration to being equivalent to 1kg dry powder/kg concentrate;
C, with the concentrate of step B gained, according to formula ratio and organic solvent, surfactant, antifreezing agent and water in proportion in the still that is mixed, the still temperature that is mixed be 35 ?60 ℃, mixing time is 30 minutes, namely gets wide arnotto red-spotted stonecrop Antiphytoviral microemulsion after stirring;
D, with the concentrate of step B gained, in the still that is mixed, the still temperature that is mixed is 35 ℃～60 ℃ according to formula ratio and organic solvent and surfactant, mixing time is 30 minutes, namely gets wide arnotto red-spotted stonecrop Antiphytoviral soluble liquid after stirring;
E, with the concentrate of step B gained, according to formula ratio and organic solvent, surfactant, antifreezing agent and water in proportion in the still that is mixed, the still temperature that is mixed is 35 ℃～60 ℃, and mixing time is 30 minutes, namely gets wide arnotto red-spotted stonecrop Antiphytoviral aqua after stirring.
4. method as claimed in claim 3 is characterized in that:
Described organic solvent is ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, the mixture of one or more in dimethyl formamide;
The mixture of one or more that described emulsifier is alkyl benzene sulfonate, alkylphenol polyoxyethylene class, multi-styrene phenol APEO, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, this Pan's series, tween, agricultural the moon in the sub-?phosphate;
Described surfactant is one or more mixture in the addition product, aralkyl phenol polyethenoxy ether formaldehyde condensation products of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane;
Described antifreezing agent is ethylene glycol or glycerine.
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| CN104872205A (en) * | 2015-05-13 | 2015-09-02 | 湖北省烟草科学研究院 | Mixture for controlling soil-borne diseases of tobaccos, and using method of mixture |
| CN104872205B (en) * | 2015-05-13 | 2017-08-25 | 湖北省烟草科学研究院 | The mixture and its application method prevented and treated for soil-borne disease of tobacco |
| CN112514896A (en) * | 2020-12-07 | 2021-03-19 | 吉林农业大学 | Rhodiola sachalinensis wettable powder and application |
| CN112514896B (en) * | 2020-12-07 | 2022-02-01 | 吉林农业大学 | Rhodiola sachalinensis wettable powder and application |
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Application publication date: 20130612 |