CN101284037A - Gadol extract and preparation method thereof - Google Patents
Gadol extract and preparation method thereof Download PDFInfo
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- CN101284037A CN101284037A CN200810112869.XA CN200810112869A CN101284037A CN 101284037 A CN101284037 A CN 101284037A CN 200810112869 A CN200810112869 A CN 200810112869A CN 101284037 A CN101284037 A CN 101284037A
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- radix rhodiolae
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- 239000000284 extract Substances 0.000 title claims abstract description 32
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 238000000605 extraction Methods 0.000 title claims description 18
- 239000012528 membrane Substances 0.000 claims abstract description 22
- 238000000926 separation method Methods 0.000 claims abstract description 21
- 238000000746 purification Methods 0.000 claims abstract description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 18
- 239000003960 organic solvent Substances 0.000 claims abstract description 16
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 15
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 11
- 238000000638 solvent extraction Methods 0.000 claims description 11
- 238000005352 clarification Methods 0.000 claims description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 9
- 229920001661 Chitosan Polymers 0.000 claims description 8
- 239000000706 filtrate Substances 0.000 claims description 8
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- -1 polypropylene Polymers 0.000 claims description 5
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 claims description 4
- 239000004743 Polypropylene Substances 0.000 claims description 3
- 239000000919 ceramic Substances 0.000 claims description 3
- 229910052751 metal Inorganic materials 0.000 claims description 3
- 239000002184 metal Substances 0.000 claims description 3
- 229920002492 poly(sulfone) Polymers 0.000 claims description 3
- 229920001155 polypropylene Polymers 0.000 claims description 3
- 239000002033 PVDF binder Substances 0.000 claims description 2
- 239000002131 composite material Substances 0.000 claims description 2
- 239000000463 material Substances 0.000 claims description 2
- 239000012046 mixed solvent Substances 0.000 claims description 2
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims description 2
- 239000012141 concentrate Substances 0.000 claims 2
- 238000003809 water extraction Methods 0.000 claims 2
- 150000004676 glycans Chemical class 0.000 abstract description 9
- 229920001282 polysaccharide Polymers 0.000 abstract description 9
- 239000005017 polysaccharide Substances 0.000 abstract description 9
- 102000004169 proteins and genes Human genes 0.000 abstract description 9
- 108090000623 proteins and genes Proteins 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 5
- 229930003944 flavone Natural products 0.000 abstract description 4
- 235000011949 flavones Nutrition 0.000 abstract description 4
- 238000000034 method Methods 0.000 abstract description 4
- 239000012535 impurity Substances 0.000 abstract description 3
- 229930182558 Sterol Natural products 0.000 abstract description 2
- 238000000354 decomposition reaction Methods 0.000 abstract description 2
- 238000003912 environmental pollution Methods 0.000 abstract description 2
- 150000008442 polyphenolic compounds Chemical class 0.000 abstract description 2
- 235000013824 polyphenols Nutrition 0.000 abstract description 2
- 150000003432 sterols Chemical class 0.000 abstract description 2
- 235000003702 sterols Nutrition 0.000 abstract description 2
- 150000003648 triterpenes Chemical class 0.000 abstract description 2
- 241001170121 Rhodiola sacra Species 0.000 abstract 3
- YCCILVSKPBXVIP-UHFFFAOYSA-N 2-(4-hydroxyphenyl)ethanol Chemical compound OCCC1=CC=C(O)C=C1 YCCILVSKPBXVIP-UHFFFAOYSA-N 0.000 abstract 2
- DBLDQZASZZMNSL-QMMMGPOBSA-N L-tyrosinol Natural products OC[C@@H](N)CC1=CC=C(O)C=C1 DBLDQZASZZMNSL-QMMMGPOBSA-N 0.000 abstract 1
- ILRCGYURZSFMEG-UHFFFAOYSA-N Salidroside Natural products OC1C(O)C(O)C(CO)OC1OCCC1=CC=C(O)C=C1 ILRCGYURZSFMEG-UHFFFAOYSA-N 0.000 abstract 1
- 229940126678 chinese medicines Drugs 0.000 abstract 1
- 150000002213 flavones Chemical class 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- ILRCGYURZSFMEG-RQICVUQASA-N salidroside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)OC1OCCC1=CC=C(O)C=C1 ILRCGYURZSFMEG-RQICVUQASA-N 0.000 abstract 1
- 235000004330 tyrosol Nutrition 0.000 abstract 1
- 239000007788 liquid Substances 0.000 description 14
- ILRCGYURZSFMEG-RKQHYHRCSA-N Salidroside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OCCC1=CC=C(O)C=C1 ILRCGYURZSFMEG-RKQHYHRCSA-N 0.000 description 4
- 206010016256 fatigue Diseases 0.000 description 4
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 3
- 208000026106 cerebrovascular disease Diseases 0.000 description 3
- 235000015961 tonic Nutrition 0.000 description 3
- 230000001256 tonic effect Effects 0.000 description 3
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 3
- 206010019233 Headaches Diseases 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 230000017531 blood circulation Effects 0.000 description 2
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- 210000004351 coronary vessel Anatomy 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
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- 229960000716 tonics Drugs 0.000 description 2
- 239000011573 trace mineral Substances 0.000 description 2
- 235000013619 trace mineral Nutrition 0.000 description 2
- 206010002383 Angina Pectoris Diseases 0.000 description 1
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- 241000976983 Anoxia Species 0.000 description 1
- 206010008190 Cerebrovascular accident Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000007443 Neurasthenia Diseases 0.000 description 1
- 235000004789 Rosa xanthina Nutrition 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- 208000013738 Sleep Initiation and Maintenance disease Diseases 0.000 description 1
- 208000006011 Stroke Diseases 0.000 description 1
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 238000003811 acetone extraction Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 208000008445 altitude sickness Diseases 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 230000004872 arterial blood pressure Effects 0.000 description 1
- 206010003549 asthenia Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000003130 cardiopathic effect Effects 0.000 description 1
- 229910017052 cobalt Inorganic materials 0.000 description 1
- 239000010941 cobalt Substances 0.000 description 1
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000002481 ethanol extraction Methods 0.000 description 1
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- 208000019622 heart disease Diseases 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 206010022437 insomnia Diseases 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 238000003808 methanol extraction Methods 0.000 description 1
- 230000004089 microcirculation Effects 0.000 description 1
- 229910052750 molybdenum Inorganic materials 0.000 description 1
- 239000011733 molybdenum Substances 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 230000036581 peripheral resistance Effects 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 235000021395 porridge Nutrition 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
- 230000003867 tiredness Effects 0.000 description 1
- 208000016255 tiredness Diseases 0.000 description 1
- 229910052719 titanium Inorganic materials 0.000 description 1
- 239000010936 titanium Substances 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a Rhodiola sacra extract and a preparation method thereof, belonging to the technology field of Chinese medicines. The method comprises the steps of: extracting Rhodiola sacra with organic solvent, and concentrating to obtain concentrated solution; ultrasonically extracting residues with water, clarifying, and concentrating to obtain concentrated solution; and mixing the concentrated solutions, and membrane separating and purifying. The obtained Rhodiola sacra extract contains further purified active components such as tyrosol, salidroside, flavones, polyphenols, triterpenes and sterols. The method effectively removes impurities such as proteins and polysaccharides by the membrane separation technique; has high separation and purification efficiency; and takes certain concentration effect. The method has the advantages of continuous room temperature operation, high efficiency, low cost, reduced environmental pollution, and no decomposition of effective components due to high temperature and long operation time.
Description
Technical field
The present invention relates to a kind of Radix Rhodiolae extract and preparation method thereof, belong to medical technical field.
Background technology
Radix Rhodiolae is the Rosaceae Rhodida plant, is a kind of medicinal herbs most in use.Have strengthening by means of tonics, holding up multiple functions such as this is solid just, resisting fatigue, anoxia enduring, cold resistance, resisting microwave radiation, and increase work efficiency, delay effect such as body aging.Radix Rhodiolae mainly contains effective constituent rhodioloside, butyl alcohol, contain in addition, tannin, flavone compound, phenolic compound and micro-volatile oil, also contain trace elements iron, aluminum, zinc, silver, cobalt, cadmium, titanium, molybdenum, manganese of biologically active etc., contain a small amount of biological alkali in leaf and the stem.Be used for resisting fatigue, defying age and aspects such as raising mentality and physical function.China is for the existing history for a long time of the use of Radix Rhodiolae, far away from the Qing Dynasty, just have in the Radix Rhodiolae producing region people with it as the strengthening by means of tonics medicine, allaying tiredness not, the cold resisting.The northeast region-by-region, among the people commonly used its as tonic and treatment disease, fry in shallow oil water or steep in wine and eliminate cold in winter of the tired and opposing high and cold mountain area that heavy physical labour brings with it.Radix Rhodiolae contains abundant rhodioside, flavone, and multivitamin and trace element, according to the pharmacology, pathology and clinical observation on the therapeutic effect, Radix Rhodiolae is to be proved to be a kind of to the heart, and cerebrovascular disease has the natural herbal of significant curative effect.It can remove lipid too much in the blood, prevents the formation of tremulous pulse medicated porridge shape speckle, reduces blood viscosity, quickens red cell velocity, microcirculation improvement, and Peripheral resistance is reduced, thus reduce arterial pressure.Radix Rhodiolae can be expanded coronary artery effectively, resists myocardial ischemia, and improves cardiac function, also can improve the blood circulation of cerebral tissue, accelerates the recovery of cerebral infarction focus, and to alleviating headache, relieving fatigue, memory reinforcing etc. also have remarkable efficacy.Take Radix Rhodiolae for a long time and can prevent or slow down apoplexy and cardiopathic generation.Radix Rhodiolae treatment and prevention coronary heart disease, hypertension, cerebrovascular, climacteric syndrome, diseases such as neurasthenia and the angina pectoris that is caused thereof, uncomfortable in chest, cardiopalmus is breathed hard, insomnia, spiritlessness and weakness, the good medicine of altitude sickness etc. also has tangible auxiliary curative effect to multiple cancer.Expand coronary artery effectively, resist myocardial ischemia, improve cardiac function, also can improve the blood circulation of cerebral tissue, accelerate the recovery of cerebral infarction focus, to alleviating headache, relieving fatigue, memory reinforcing etc. also have remarkable efficacy.
The present invention overcomes the prior art deficiency, and a kind of Radix Rhodiolae extract and preparation method thereof is provided.
Summary of the invention
The object of the invention provides a kind of Radix Rhodiolae extract and preparation method thereof.
Herb Gynostemmae Pentaphylli extract provided by the invention makes by the following technical programs:
Herb Gynostemmae Pentaphylli is pulverized, with 1～10 times organic solvent extraction 1～3 time, each 1～3h, organic solvent concentration 50%～80%, the medicinal residues reservation is standby, merge extractive liquid,, concentrating under reduced pressure is concentrated into relative density and is 1.05～1.40 concentrated solution; Medicinal residues are added 10～20 times of water supersound extraction 1～3 time, each 1～3h, supersonic frequency 20～80kHz extracts 70～90 ℃ of temperature, filters, and filtrate adds 0.02%～0.2% chitosan clarification, 2～4h, filters, and is concentrated into relative density and is 1.05～1.40 concentrated solution; Organic solvent extraction concentrated solution and water extracting liquid are merged, and membrane separation purification is removed impurity such as protein, polysaccharide, promptly gets Radix Rhodiolae extract of the present invention;
Described organic solvent is one or more the mixed solvent in methanol, ethanol, propanol, isopropyl alcohol, n-butyl alcohol, the acetone;
Described film is one or more composite film materials in polysulfone membrane, polypropylene screen, polysulfonamides film, metal film, ceramic membrane, the polyvinylidene fluoride film;
Pressure is 0.1～1.5MPa during described membrane separation purification.
The advantage of the technology of the present invention is, adopt membrane separation and purification technology to remove impurity such as protein, polysaccharide effectively, make Radix Rhodiolae main active butyl alcohol, rhodioloside, flavone, polyphenol, triterpene, sterol etc. obtain further purification, significantly improved separation and purification efficient; And play certain concentrated effect; More existing extractive technique has improved efficient, has saved cost, has reduced environmental pollution; The technology of the present invention is continued operation at normal temperatures, has safety, characteristics efficiently and cheaply; The decomposition of the effective ingredient that avoided conventional isolation technics Yin Gaowen, causes for a long time; Therefore the more conventional isolation technics of membrane separation technique of the present invention has significant advantage.
The specific embodiment
The following examples help those skilled in the art more fully to understand the present invention, but do not limit the present invention in any way.
Embodiment 1
Herb Gynostemmae Pentaphylli 10kg is pulverized, with 8 times ethanol extractions 2 times, each 2h, concentration of alcohol 60%, the medicinal residues reservation is standby, merge extractive liquid,, concentrating under reduced pressure is concentrated into relative density and is 1.05～1.40 concentrated solution; Medicinal residues are added 15 times of water supersound extraction 2 times, each 1h, supersonic frequency 60kHz extracts 80 ℃ of temperature, filters, and filtrate adds 0.1% chitosan clarification 3h, filters, and is concentrated into relative density and is 1.05～1.40 concentrated solution; Organic solvent extraction concentrated solution and water extracting liquid are merged, the polysulfone membrane separation and purification, pressure is 1.5MPa during membrane separation purification, promptly gets Radix Rhodiolae extract of the present invention.The protein removal rate is 93.8%, and the polysaccharide clearance is 95.4%.
Embodiment 2
Herb Gynostemmae Pentaphylli 10kg is pulverized, with 6 times acetone extractions 3 times, each 1h, acetone concentration 80%, the medicinal residues reservation is standby, merge extractive liquid,, concentrating under reduced pressure is concentrated into relative density and is 1.05～1.40 concentrated solution; Medicinal residues are added 20 times of water supersound extraction 1 time, each 1h, supersonic frequency 40kHz extracts 70 ℃ of temperature, filters, and filtrate adds 0.15% chitosan clarification 3h, filters, and is concentrated into relative density and is 1.05～1.40 concentrated solution; Organic solvent extraction concentrated solution and water extracting liquid are merged, the Kynoar membrane separation purification, pressure is 0.8MPa during membrane separation purification, promptly gets Radix Rhodiolae extract of the present invention.The protein removal rate is 95.1%, and the polysaccharide clearance is 96.7%.
Embodiment 3
Herb Gynostemmae Pentaphylli 30kg is pulverized, with 4 times propanol extraction 3 times, each 2h, propanol concentration 50%, the medicinal residues reservation is standby, merge extractive liquid,, concentrating under reduced pressure is concentrated into relative density and is 1.05～1.40 concentrated solution; Medicinal residues are added 20 times of water supersound extraction 1 time, each 1h, supersonic frequency 20kHz extracts 70 ℃ of temperature, filters, and filtrate adds 0.15% chitosan clarification 3h, filters, and is concentrated into relative density and is 1.05～1.40 concentrated solution; Organic solvent extraction concentrated solution and water extracting liquid are merged, the polypropylene screen separation and purification, pressure is 1.2MPa during membrane separation purification, promptly gets Radix Rhodiolae extract of the present invention.The protein removal rate is 92.1%, and the polysaccharide clearance is 93.5%.
Embodiment 4
Herb Gynostemmae Pentaphylli 50kg is pulverized, with 10 times methanol extraction 2 times, each 1h, methanol concentration 60%, the medicinal residues reservation is standby, merge extractive liquid,, concentrating under reduced pressure is concentrated into relative density and is 1.05～1.40 concentrated solution; Medicinal residues are added 10 times of water supersound extraction 3 times, each 2h, supersonic frequency 50kHz extracts 80 ℃ of temperature, filters, and filtrate adds 0.12% chitosan clarification 2h, filters, and is concentrated into relative density and is 1.05～1.40 concentrated solution; Organic solvent extraction concentrated solution and water extracting liquid are merged, the metal film separation and purification, pressure is 1.0MPa during membrane separation purification, promptly gets Radix Rhodiolae extract of the present invention.The protein removal rate is 92.8%, and the polysaccharide clearance is 92.3%.
Embodiment 5
Herb Gynostemmae Pentaphylli 50kg is pulverized, with 10 times n-butanol extractions 2 times, each 1h, n-butyl alcohol concentration 60%, the medicinal residues reservation is standby, merge extractive liquid,, concentrating under reduced pressure is concentrated into relative density and is 1.05～1.40 concentrated solution; Medicinal residues are added 10 times of water supersound extraction 3 times, each 2h, supersonic frequency 50 kHz extract 80 ℃ of temperature, filter, and filtrate adds 0.12% chitosan clarification 2h, filters, and is concentrated into relative density and is 1.05～1.40 concentrated solution; Organic solvent extraction concentrated solution and water extracting liquid are merged, the ceramic membrane separation and purification, pressure is 1.0MPa during membrane separation purification, promptly gets Radix Rhodiolae extract of the present invention.The protein removal rate is 92.8%, and the polysaccharide clearance is 92.3%.
Embodiment 6
Herb Gynostemmae Pentaphylli 50kg is pulverized, with 10 times isopropanol extractions 2 times, each 1h, isopropyl alcohol concentration 60%, the medicinal residues reservation is standby, merge extractive liquid,, concentrating under reduced pressure is concentrated into relative density and is 1.05～1.40 concentrated solution; Medicinal residues are added 10 times of water supersound extraction 3 times, each 2h, supersonic frequency 50kHz extracts 80 ℃ of temperature, filters, and filtrate adds 0.12% chitosan clarification 2h, filters, and is concentrated into relative density and is 1.05～1.40 concentrated solution; Organic solvent extraction concentrated solution and water extracting liquid are merged, the polysulfonamides membrane separation purification, pressure is 1.0MPa during membrane separation purification, promptly gets Radix Rhodiolae extract of the present invention.The protein removal rate is 90.5%, and the polysaccharide clearance is 95.2%.
Claims (8)
1. Radix Rhodiolae extract and preparation method thereof is characterized in that, Herb Gynostemmae Pentaphylli is used organic solvent extraction, concentrate concentrated solution; Medicinal residues add the water supersound extraction, clarification, concentrate concentrated solution; Two kinds of concentrated solutions merge, and membrane separation purification promptly gets Radix Rhodiolae extract of the present invention.
2. a kind of Radix Rhodiolae extract according to claim 1 and preparation method thereof is characterized in that, described organic solvent is one or more the mixed solvent in methanol, ethanol, propanol, isopropyl alcohol, n-butyl alcohol, the acetone.
3. a kind of Radix Rhodiolae extract according to claim 1 and preparation method thereof is characterized in that, described film is one or more composite film materials in polysulfone membrane, polypropylene screen, polysulfonamides film, metal film, ceramic membrane, the polyvinylidene fluoride film.
4. a kind of Radix Rhodiolae extract according to claim 1 and preparation method thereof is characterized in that, pressure is 0.1～1.5MPa during described membrane separation purification.
5. a kind of Radix Rhodiolae extract according to claim 1 and preparation method thereof is characterized in that, described organic solvent extraction is with 1～10 times organic solvent extraction 1～3 time, each 1～3h, organic solvent concentration 50%～80% with Radix Rhodiolae.
6. a kind of Radix Rhodiolae extract according to claim 1 and preparation method thereof is characterized in that, described water extraction is for to add 10～20 times of water supersound extraction 1～3 time with medicinal residues, each 1～3h, and supersonic frequency 20～80kHz extracts 70～90 ℃ of temperature.
7. a kind of Radix Rhodiolae extract according to claim 1 and preparation method thereof is characterized in that, described clarification adds 0.02%～0.2% chitosan clarification, 2～4h for water extraction filtrate.
8. a kind of Radix Rhodiolae extract according to claim 1 and preparation method thereof is characterized in that, it is 1.05～1.40 that described concentrated solution is concentrated into relative density.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810112869.XA CN101284037A (en) | 2008-05-26 | 2008-05-26 | Gadol extract and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810112869.XA CN101284037A (en) | 2008-05-26 | 2008-05-26 | Gadol extract and preparation method thereof |
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| CN101284037A true CN101284037A (en) | 2008-10-15 |
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| CN200810112869.XA Pending CN101284037A (en) | 2008-05-26 | 2008-05-26 | Gadol extract and preparation method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103141525A (en) * | 2013-02-06 | 2013-06-12 | 杨凌农科大无公害农药研究服务中心 | Rhodiola eurycarpa anti-plant virus agent and preparation method thereof |
| CN103923132A (en) * | 2014-04-16 | 2014-07-16 | 安徽博格生物科技有限公司 | Ultrasonic extraction technology for salidroside in natural Rhodiola rosea |
| CN104628528A (en) * | 2015-01-05 | 2015-05-20 | 康美（北京）药物研究院有限公司 | Preparation method of tyrosol |
-
2008
- 2008-05-26 CN CN200810112869.XA patent/CN101284037A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103141525A (en) * | 2013-02-06 | 2013-06-12 | 杨凌农科大无公害农药研究服务中心 | Rhodiola eurycarpa anti-plant virus agent and preparation method thereof |
| CN103923132A (en) * | 2014-04-16 | 2014-07-16 | 安徽博格生物科技有限公司 | Ultrasonic extraction technology for salidroside in natural Rhodiola rosea |
| CN104628528A (en) * | 2015-01-05 | 2015-05-20 | 康美（北京）药物研究院有限公司 | Preparation method of tyrosol |
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